FvH4_5g25490

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000356
Physical Location & Seq
Forward (+)
16964592 .. 16965833
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g25490.t1

Sequence Viewer

Length: 1242 bp
ATGGCGCCGGAGTGTCACCGGCACCACCCTTATAGCCGTCCTCAACCGTCGTTCAAGGCATCTGGTCCGATTCGGCACCAAACCCAAGTCGCCTCCGTCGTCCGATACGCCCAGAACCGGATGTCGTCGGCGGTTGTTTATTCCGGCATCCCATCAAGGTCGGCGCCGGCTTCAAGGCTTGTACGTTGTTGTAGAAATTACAGGACCCGCGCTTCTGCGATCAATCGGGTTCTGTGGACACCTTCCGGCAGGCTGATCACCGGCTCCCGAAACGGCGAGTTCACTGTTTGGGATGGCCTCTCGTTTCGCCATGAATTGACCGTTGAGGAGGCTCATGATCATTCCATCAGGTCCATGGTGTGGAGTTTTGAACATGAAGACTGGATCTCTGGTGATGATGGGGGATGCATCAAGTACTGGAAGACCAACATGACCAATGTGCTTGTCAAACAATCTGCTCACCAGGAAGCCGTTCGCGACTTGAGCTTCTGCAGGACCAATTTGAAGTTCTGCTCCTGTTCGGACGATACACTTGTTAAGGTCTGGGATTATGAGACCTGCCAAGAGGAGCGCAGATTGACTGGCCATAGTTGGAATGTGACCAGTGTCGACTGGCACCCTACAAAGTCTCTGGTAGCCTCTGGTGGGAAGGACGGTGTTGTCAAGCTGTGGGATGCAAGGACAGCAAGAGAGCTTTGCTCTTTTTATGATCACAACAGTTGGGTCAACTCTGTCAAGTGGAACCAAAACGGTAACTGGCTCCTCACTGCTTCCAAGGATCAGGTTATCAAGCTTTATGATATAAGGTCTATGAAGGAGCTCCTTTCGTTCCGCGGCCATTGCGGCCCAGTGACTGCTCTAGCTTGGCATCCTTTACAGGAAGACTATCTTGTCAGTGGGACTGGTGATGGATCCATTCTTCATTGGCTAGTTGGGCATCTATCTCCCCAGGTTCAACTTCCTAATGCACACAATAGCAATAACTATAATAACAGTGTGTGGGATTTGAAGTGGCATCCTATGGGTCATATGCTTTGCAGTGGCAGCAATGATCGCACTACTAAGTTTTGGTGTAACAATGATGAATCTGATATTAATGAGAATCAAAGTTTTGGTGTTCTAAATTCTGCTTTTGCTGCTTACATGTCTTGTAGTTTTCCTTTACCATTTCATGAAGAACAACCTTCACTCGTTGCTAAGAGAGAAGGAACCGTTATTCCAGGTGTTGGGATTGCAATTTAA

Protein Analysis

414

Amino Acids

46.78

Weight (kDa)

7.95

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 47 - 277 1.7e-20 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 62 - 192 7.2e-14 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 62 - 186 1.8e-10 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 66 - 242 8.8e-25 THOC3 beta-propeller domain
Beta-prop_IFT140_1st PF23383 69 - 158 4.7e-06 IFT140 first beta-propeller
WD40_Prp19 PF24814 83 - 274 5.3e-27 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 85 - 183 7.6e-07 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 93 - 230 5.2e-22 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 102 - 225 1.7e-09 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 109 - 189 6.8e-07 EIF3I
Beta-prop_TEP1_2nd PF25047 117 - 287 1.8e-14 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 136 - 240 8.2e-21 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 137 - 226 8.6e-10 WDR3 second beta-propeller domain
WDR55 PF24796 145 - 310 3.5e-19 WDR55
Beta-prop_WDR36-Utp21_1st PF25171 147 - 358 5.4e-14 WDR36/Utp21 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 149 - 309 2.8e-17 WDR36/Utp21 second beta-propeller domain
Beta-prop_SCAP PF24017 151 - 247 3e-06 SCAP Beta-propeller
WD40_WDHD1_1st PF24817 155 - 232 5.5e-11 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 166 - 314 9.6e-23 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 170 - 257 2.9e-08 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_2nd PF25172 171 - 267 2.4e-13 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 176 - 309 1.8e-24 CDC20/Fizzy WD40 domain
Beta-prop_IFT140_1st PF23383 179 - 263 2.4e-07 IFT140 first beta-propeller
WD40 PF00400 190 - 225 2.9e-10 WD domain, G-beta repeat
Beta-prop_EML PF23409 191 - 309 8.7e-10 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 192 - 272 3.5e-09 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5 PF25175 193 - 357 1.2e-25 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 193 - 357 4.4e-27 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 193 - 309 1.2e-11 WDR3 second beta-propeller domain
EIF3I PF24805 197 - 277 6.6e-10 EIF3I
WD40_MABP1-WDR62_2nd PF24782 210 - 357 4.3e-09 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 231 - 365 2.6e-18 CDC20/Fizzy WD40 domain
WD40 PF00400 232 - 267 1.1e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 238 - 366 5e-21 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 239 - 362 1.7e-06 Aladin seven-bladed propeller
WD40_WDHD1_1st PF24817 241 - 346 3.5e-13 WDHD1 first WD40 domain
WD40_Prp19 PF24814 277 - 357 9.3e-07 Prp19 WD40 domain
WD40 PF00400 330 - 357 9.6e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica Prupe.2G034300_v2.0.a1
pyrus_communis pycom02g24420 pycom07g02650
rosa_chinensis RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 659, 890
Acc36I ACCTGC 1 cut(s) 566
AccB1I GGYRCC 5 cut(s) 4, 21, 75, 163, 615
AccB7I CCANNNNNTGG 2 cut(s) 360, 1226
AccI GTMKAC 1 cut(s) 609
AccII CGCG 3 cut(s) 210, 477, 834
AciI CCGC 5 cut(s) 131, 208, 832, 834, 843
AclWI GGATC 4 cut(s) 392, 786, 906, 919
AcoI YGGCCR 2 cut(s) 583, 835
AcsI RAATTY 1 cut(s) 1123
AcyI GRCGYC 2 cut(s) 5, 164
AfaI GTAC 2 cut(s) 183, 416
AfiI CCNNNNNNNGG 4 cut(s) 117, 360, 645, 1226
AflIII ACRYGT 1 cut(s) 1143
AgsI TTSAA 6 cut(s) 55, 174, 371, 505, 956, 1009
AjnI CCWGG 3 cut(s) 462, 948, 1219
AjuI GAANNNNNNNTTGG 2 cut(s) 491, 523
AloI GAACNNNNNNTCC 2 cut(s) 1201, 1233
AluBI AGCT 6 cut(s) 486, 667, 694, 793, 820, 863
AluI AGCT 6 cut(s) 486, 667, 694, 793, 820, 863
Alw21I GWGCWC 1 cut(s) 822
Alw26I GTCTC 2 cut(s) 548, 633
AlwI GGATC 4 cut(s) 392, 786, 906, 919
AlwNI CAGNNNCTG 1 cut(s) 854
AoxI GGCC 4 cut(s) 295, 583, 835, 844
ApeKI GCWGC 2 cut(s) 1044, 1136
ApoI RAATTY 1 cut(s) 1123
AseI ATTAAT 1 cut(s) 1095
Asp700I GAANNNNTTC 1 cut(s) 471
AspLEI GCGC 4 cut(s) 7, 166, 212, 573
AspS9I GGNCC 5 cut(s) 65, 204, 351, 495, 845
AsuHPI GGTGA 5 cut(s) 8, 250, 404, 452, 917
AvaII GGWCC 4 cut(s) 65, 204, 351, 495
BaeI ACNNNNGTAYC 2 cut(s) 519, 552
BalI TGGCCA 1 cut(s) 585
BamHI GGATCC 1 cut(s) 911
BanI GGYRCC 5 cut(s) 4, 21, 75, 163, 615
BanII GRGCYC 1 cut(s) 822
BbsI GAAGAC 3 cut(s) 384, 428, 888
Bbv12I GWGCWC 1 cut(s) 822
BbvI GCAGC 2 cut(s) 1056, 1123
BccI CCATC 5 cut(s) 160, 287, 353, 392, 902
BceAI ACGGC 3 cut(s) 21, 289, 455
BciT130I CCWGG 3 cut(s) 464, 950, 1221
BclI TGATCA 3 cut(s) 255, 337, 709
BcoDI GTCTC 2 cut(s) 548, 633
BfaI CTAG 2 cut(s) 860, 929
BfmI CTRYAG 1 cut(s) 490
BfoI RGCGCY 2 cut(s) 8, 167
BfuAI ACCTGC 1 cut(s) 566
BglI GCCNNNNNGGC 1 cut(s) 843
BisI GCNGC 4 cut(s) 835, 844, 1045, 1137
BlsI GCNGC 4 cut(s) 836, 845, 1046, 1138
BmcAI AGTACT 1 cut(s) 416
Bme1390I CCNGG 3 cut(s) 464, 950, 1221
Bme18I GGWCC 4 cut(s) 65, 204, 351, 495
BmgT120I GGNCC 5 cut(s) 65, 204, 351, 495, 845
BmrFI CCNGG 3 cut(s) 464, 950, 1221
BmrI ACTGGG 1 cut(s) 842
BmsI GCATC 8 cut(s) 68, 156, 395, 417, 664, 877, 946, 1024
BmuI ACTGGG 1 cut(s) 842
BoxI GACNNNNGTC 1 cut(s) 605
BpiI GAAGAC 3 cut(s) 384, 428, 888
BplI GAGNNNNNCTC 2 cut(s) 683, 715
BpuEI CTTGAG 1 cut(s) 502
BsaHI GRCGYC 2 cut(s) 5, 164
BsaI GGTCTC 1 cut(s) 548
BsaJI CCNNGG 4 cut(s) 354, 774, 832, 948
BsaWI WCCGGW 1 cut(s) 117
Bsc4I CCNNNNNNNGG 4 cut(s) 117, 360, 645, 1226
Bse118I RCCGGY 3 cut(s) 18, 166, 260
Bse1I ACTGG 8 cut(s) 386, 422, 586, 603, 617, 761, 848, 907
Bse3DI GCAATG 2 cut(s) 838, 1054
BseBI CCWGG 3 cut(s) 464, 950, 1221
BseDI CCNNGG 4 cut(s) 354, 774, 832, 948
BseGI GGATG 7 cut(s) 126, 147, 298, 410, 679, 868, 1015
BseLI CCNNNNNNNGG 4 cut(s) 117, 360, 645, 1226
BseMI GCAATG 2 cut(s) 838, 1054
BseNI ACTGG 8 cut(s) 386, 422, 586, 603, 617, 761, 848, 907
BseRI GAGGAG 3 cut(s) 341, 581, 752
BseXI GCAGC 2 cut(s) 1056, 1123
Bsh1236I CGCG 3 cut(s) 210, 477, 834
BshFI GGCC 4 cut(s) 297, 585, 837, 846
BshNI GGYRCC 5 cut(s) 4, 21, 75, 163, 615
BsiHKAI GWGCWC 1 cut(s) 822
BsiSI CCGG 7 cut(s) 8, 19, 118, 144, 167, 246, 261
BslFI GGGAC 1 cut(s) 913
BslI CCNNNNNNNGG 4 cut(s) 117, 360, 645, 1226
BsmAI GTCTC 2 cut(s) 548, 633
BsmFI GGGAC 1 cut(s) 913
BsnI GGCC 4 cut(s) 297, 585, 837, 846
Bso31I GGTCTC 1 cut(s) 548
Bsp1286I GDGCHC 1 cut(s) 822
Bsp143I GATC 8 cut(s) 219, 255, 337, 384, 709, 778, 911, 1051
Bsp19I CCATGG 1 cut(s) 354
Bsp68I TCGCGA 1 cut(s) 477
BspACI CCGC 5 cut(s) 131, 208, 832, 834, 843
BspANI GGCC 4 cut(s) 297, 585, 837, 846
BspFNI CGCG 3 cut(s) 210, 477, 834
BspHI TCATGA 2 cut(s) 334, 1171
BspMAI CTGCAG 1 cut(s) 494
BspMI ACCTGC 1 cut(s) 566
BspPI GGATC 4 cut(s) 392, 786, 906, 919
BspT107I GGYRCC 5 cut(s) 4, 21, 75, 163, 615
BspTNI GGTCTC 1 cut(s) 548
BsrDI GCAATG 2 cut(s) 838, 1054
BsrFI RCCGGY 3 cut(s) 18, 166, 260
BsrI ACTGG 8 cut(s) 386, 422, 586, 603, 617, 761, 848, 907
BssAI RCCGGY 3 cut(s) 18, 166, 260
BssECI CCNNGG 4 cut(s) 354, 774, 832, 948
BssMI GATC 8 cut(s) 219, 255, 337, 384, 709, 778, 911, 1051
BssNI GRCGYC 2 cut(s) 5, 164
BssT1I CCWWGG 2 cut(s) 354, 774
Bst2UI CCWGG 3 cut(s) 464, 950, 1221
Bst4CI ACNGT 8 cut(s) 48, 286, 322, 656, 719, 752, 995, 1213
BstACI GRCGYC 2 cut(s) 5, 164
BstC8I GCNNGC 2 cut(s) 168, 251
BstDEI CTNAG 2 cut(s) 1062, 1197
BstDSI CCRYGG 2 cut(s) 354, 832
BstF5I GGATG 7 cut(s) 126, 147, 298, 410, 679, 868, 1015
BstFNI CGCG 3 cut(s) 210, 477, 834
BstH2I RGCGCY 2 cut(s) 8, 167
BstHHI GCGC 4 cut(s) 7, 166, 212, 573
BstKTI GATC 8 cut(s) 222, 258, 340, 387, 712, 781, 914, 1054
BstMAI GTCTC 2 cut(s) 548, 633
BstMBI GATC 8 cut(s) 219, 255, 337, 384, 709, 778, 911, 1051
BstMWI GCNNNNNNNGC 8 cut(s) 483, 683, 840, 843, 934, 1044, 1053, 1136
BstNI CCWGG 3 cut(s) 464, 950, 1221
BstNSI RCATGY 1 cut(s) 1147
BstPAI GACNNNNGTC 1 cut(s) 605
BstSCI CCNGG 3 cut(s) 462, 948, 1219
BstSFI CTRYAG 1 cut(s) 490
BstUI CGCG 3 cut(s) 210, 477, 834
BstV1I GCAGC 2 cut(s) 1056, 1123
BstV2I GAAGAC 3 cut(s) 384, 428, 888
BstX2I RGATCY 2 cut(s) 384, 911
BstYI RGATCY 2 cut(s) 384, 911
BsuRI GGCC 4 cut(s) 297, 585, 837, 846
BtgI CCRYGG 2 cut(s) 354, 832
BtsCI GGATG 7 cut(s) 126, 147, 298, 410, 679, 868, 1015
BtsI GCAGTG 2 cut(s) 765, 1045
BtsIMutI CAGTG 7 cut(s) 282, 610, 765, 855, 901, 1000, 1045
BtuMI TCGCGA 1 cut(s) 477
BveI ACCTGC 1 cut(s) 566
Cac8I GCNNGC 2 cut(s) 168, 251
CaiI CAGNNNCTG 1 cut(s) 854
CciI TCATGA 2 cut(s) 334, 1171
CfoI GCGC 4 cut(s) 7, 166, 212, 573
Cfr10I RCCGGY 3 cut(s) 18, 166, 260
Cfr13I GGNCC 5 cut(s) 65, 204, 351, 495, 845
Cfr42I CCGCGG 1 cut(s) 835
Csp6I GTAC 2 cut(s) 182, 415
CviAII CATG 7 cut(s) 311, 335, 355, 374, 430, 1144, 1172
CviQI GTAC 2 cut(s) 182, 415
DdeI CTNAG 2 cut(s) 1062, 1197
DinI GGCGCC 2 cut(s) 6, 165
DpnI GATC 8 cut(s) 221, 257, 339, 386, 711, 780, 913, 1053
DpnII GATC 8 cut(s) 219, 255, 337, 384, 709, 778, 911, 1051
DrdI GACNNNNNNGTC 2 cut(s) 659, 890
DseDI GACNNNNNNGTC 2 cut(s) 659, 890
EaeI YGGCCR 2 cut(s) 583, 835
Ecl136II GAGCTC 1 cut(s) 820
Eco130I CCWWGG 2 cut(s) 354, 774
Eco24I GRGCYC 1 cut(s) 822
Eco31I GGTCTC 1 cut(s) 548
Eco47I GGWCC 4 cut(s) 65, 204, 351, 495
Eco53kI GAGCTC 1 cut(s) 820
EcoICRI GAGCTC 1 cut(s) 820
EcoO109I RGGNCCY 1 cut(s) 204
EcoRII CCWGG 3 cut(s) 462, 948, 1219
EcoT14I CCWWGG 2 cut(s) 354, 774
EcoT22I ATGCAT 1 cut(s) 410
EcoT38I GRGCYC 1 cut(s) 822
EgeI GGCGCC 2 cut(s) 6, 165
EheI GGCGCC 2 cut(s) 6, 165
ErhI CCWWGG 2 cut(s) 354, 774
FaeI CATG 7 cut(s) 314, 338, 358, 377, 433, 1147, 1175
FalI AAGNNNNNCTT 2 cut(s) 873, 905
FaqI GGGAC 1 cut(s) 913
FatI CATG 7 cut(s) 310, 334, 354, 373, 429, 1143, 1171
FauI CCCGC 1 cut(s) 215
FauNDI CATATG 1 cut(s) 1029
FbaI TGATCA 3 cut(s) 255, 337, 709
FblI GTMKAC 1 cut(s) 609
Fnu4HI GCNGC 4 cut(s) 835, 844, 1045, 1137
FokI GGATG 7 cut(s) 133, 134, 305, 417, 686, 855, 1002
FriOI GRGCYC 1 cut(s) 822
Fsp4HI GCNGC 4 cut(s) 835, 844, 1045, 1137
FspBI CTAG 2 cut(s) 860, 929
GlaI GCGC 4 cut(s) 6, 165, 211, 572
GluI GCNGC 4 cut(s) 835, 844, 1045, 1137
HaeII RGCGCY 2 cut(s) 8, 167
HaeIII GGCC 4 cut(s) 297, 585, 837, 846
HapII CCGG 7 cut(s) 8, 19, 118, 144, 167, 246, 261
HhaI GCGC 4 cut(s) 7, 166, 212, 573
Hin1I GRCGYC 2 cut(s) 5, 164
Hin1II CATG 7 cut(s) 314, 338, 358, 377, 433, 1147, 1175
Hin6I GCGC 4 cut(s) 5, 164, 210, 571
HinP1I GCGC 4 cut(s) 5, 164, 210, 571
HincII GTYRAC 2 cut(s) 610, 727
HindII GTYRAC 2 cut(s) 610, 727
HindIII AAGCTT 1 cut(s) 791
HinfI GANTC 3 cut(s) 70, 1085, 1102
HpaII CCGG 7 cut(s) 8, 19, 118, 144, 167, 246, 261
HphI GGTGA 5 cut(s) 8, 250, 404, 452, 917
Hpy166II GTNNAC 4 cut(s) 237, 282, 610, 727
Hpy188I TCNGA 4 cut(s) 69, 104, 523, 1090
Hpy188III TCNNGA 4 cut(s) 267, 335, 476, 1172
Hpy8I GTNNAC 4 cut(s) 237, 282, 610, 727
Hpy99I CGWCG 3 cut(s) 52, 101, 130
HpyAV CCTTC 5 cut(s) 252, 643, 808, 1194, 1199
HpyCH4III ACNGT 8 cut(s) 48, 286, 322, 656, 719, 752, 995, 1213
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 6 cut(s) 408, 492, 677, 968, 1038, 1235
HpyF10VI GCNNNNNNNGC 8 cut(s) 483, 683, 840, 843, 934, 1044, 1053, 1136
HpyF3I CTNAG 2 cut(s) 1062, 1197
HpySE526I ACGT 1 cut(s) 184
Hsp92I GRCGYC 2 cut(s) 5, 164
Hsp92II CATG 7 cut(s) 314, 338, 358, 377, 433, 1147, 1175
HspAI GCGC 4 cut(s) 5, 164, 210, 571
KasI GGCGCC 2 cut(s) 4, 163
KroI GCCGGC 1 cut(s) 166
KroNI GCCGGC 1 cut(s) 168
Ksp22I TGATCA 3 cut(s) 255, 337, 709
KspI CCGCGG 1 cut(s) 835
Kzo9I GATC 8 cut(s) 219, 255, 337, 384, 709, 778, 911, 1051
LmnI GCTCC 6 cut(s) 269, 518, 568, 765, 817, 825
Lsp1109I GCAGC 2 cut(s) 1056, 1123
LweI GCATC 8 cut(s) 68, 156, 395, 417, 664, 877, 946, 1024
MaeI CTAG 2 cut(s) 860, 929
MaeII ACGT 1 cut(s) 184
MaeIII GTNAC 5 cut(s) 14, 598, 752, 850, 1073
MalI GATC 8 cut(s) 221, 257, 339, 386, 711, 780, 913, 1053
MboI GATC 8 cut(s) 219, 255, 337, 384, 709, 778, 911, 1051
MboII GAAGA 5 cut(s) 389, 433, 893, 911, 1187
MflI RGATCY 2 cut(s) 384, 911
MhlI GDGCHC 1 cut(s) 822
MlsI TGGCCA 1 cut(s) 585
MluCI AATT 5 cut(s) 196, 314, 499, 1123, 1236
MluNI TGGCCA 1 cut(s) 585
Mly113I GGCGCC 2 cut(s) 5, 164
MmeI TCCRAC 1 cut(s) 572
MnlI CCTC 8 cut(s) 51, 103, 308, 319, 322, 559, 649, 773
Mox20I TGGCCA 1 cut(s) 585
Mph1103I ATGCAT 1 cut(s) 410
MroNI GCCGGC 1 cut(s) 166
MroXI GAANNNNTTC 1 cut(s) 471
MscI TGGCCA 1 cut(s) 585
MseI TTAA 3 cut(s) 537, 1095, 1240
Msp20I TGGCCA 1 cut(s) 585
MspA1I CMGCKG 1 cut(s) 834
MspI CCGG 7 cut(s) 8, 19, 118, 144, 167, 246, 261
MspR9I CCNGG 3 cut(s) 464, 950, 1221
MvaI CCWGG 3 cut(s) 464, 950, 1221
MvnI CGCG 3 cut(s) 210, 477, 834
MwoI GCNNNNNNNGC 8 cut(s) 483, 683, 840, 843, 934, 1044, 1053, 1136
NaeI GCCGGC 1 cut(s) 168
NarI GGCGCC 2 cut(s) 5, 164
NcoI CCATGG 1 cut(s) 354
NdeI CATATG 1 cut(s) 1029
NdeII GATC 8 cut(s) 219, 255, 337, 384, 709, 778, 911, 1051
NgoMIV GCCGGC 1 cut(s) 166
NlaIII CATG 7 cut(s) 314, 338, 358, 377, 433, 1147, 1175
NmuCI GTSAC 3 cut(s) 14, 598, 850
NruI TCGCGA 1 cut(s) 477
NsiI ATGCAT 1 cut(s) 410
NspI RCATGY 1 cut(s) 1147
PagI TCATGA 2 cut(s) 334, 1171
PciI ACATGT 1 cut(s) 1143
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PdiI GCCGGC 1 cut(s) 168
PdmI GAANNNNTTC 1 cut(s) 471
PfeI GAWTC 3 cut(s) 70, 1085, 1102
PflMI CCANNNNNTGG 2 cut(s) 360, 1226
PkrI GCNGC 4 cut(s) 836, 845, 1046, 1138
PluTI GGCGCC 2 cut(s) 8, 167
PpuMI RGGWCCY 1 cut(s) 204
PscI ACATGT 1 cut(s) 1143
PshAI GACNNNNGTC 1 cut(s) 605
PshBI ATTAAT 1 cut(s) 1095
Psp124BI GAGCTC 1 cut(s) 822
Psp5II RGGWCCY 1 cut(s) 204
Psp6I CCWGG 3 cut(s) 462, 948, 1219
PspGI CCWGG 3 cut(s) 462, 948, 1219
PspPI GGNCC 5 cut(s) 65, 204, 351, 495, 845
PspPPI RGGWCCY 1 cut(s) 204
PstI CTGCAG 1 cut(s) 494
PstNI CAGNNNCTG 1 cut(s) 854
PsuI RGATCY 2 cut(s) 384, 911
RruI TCGCGA 1 cut(s) 477
RsaI GTAC 2 cut(s) 183, 416
RsaNI GTAC 2 cut(s) 182, 415
SacI GAGCTC 1 cut(s) 822
SacII CCGCGG 1 cut(s) 835
SalI GTCGAC 1 cut(s) 608
SaqAI TTAA 3 cut(s) 537, 1095, 1240
SatI GCNGC 4 cut(s) 835, 844, 1045, 1137
Sau3AI GATC 8 cut(s) 219, 255, 337, 384, 709, 778, 911, 1051
Sau96I GGNCC 5 cut(s) 65, 204, 351, 495, 845
ScaI AGTACT 1 cut(s) 416
ScrFI CCNGG 3 cut(s) 464, 950, 1221
SduI GDGCHC 1 cut(s) 822
SfaNI GCATC 8 cut(s) 68, 156, 395, 417, 664, 877, 946, 1024
SfcI CTRYAG 1 cut(s) 490
SfiI GGCCNNNNNGGCC 1 cut(s) 843
SfoI GGCGCC 2 cut(s) 6, 165
Sfr303I CCGCGG 1 cut(s) 835
SgrBI CCGCGG 1 cut(s) 835
SinI GGWCC 4 cut(s) 65, 204, 351, 495
SmlI CTYRAG 1 cut(s) 481
SmoI CTYRAG 1 cut(s) 481
Sse9I AATT 5 cut(s) 196, 314, 499, 1123, 1236
SsiI CCGC 5 cut(s) 131, 208, 832, 834, 843
SspDI GGCGCC 2 cut(s) 4, 163
SspMI CTAG 2 cut(s) 860, 929
SstI GAGCTC 1 cut(s) 822
StyD4I CCNGG 3 cut(s) 462, 948, 1219
StyI CCWWGG 2 cut(s) 354, 774
TaaI ACNGT 8 cut(s) 48, 286, 322, 656, 719, 752, 995, 1213
TaiI ACGT 1 cut(s) 187
TaqI TCGA 1 cut(s) 609
TasI AATT 5 cut(s) 196, 314, 499, 1123, 1236
TatI WGTACW 1 cut(s) 414
TauI GCSGC 2 cut(s) 837, 846
TfiI GAWTC 3 cut(s) 70, 1085, 1102
Tru1I TTAA 3 cut(s) 537, 1095, 1240
Tru9I TTAA 3 cut(s) 537, 1095, 1240
TscAI CASTG 7 cut(s) 289, 610, 772, 855, 901, 1000, 1045
TseFI GTSAC 3 cut(s) 14, 598, 850
TseI GCWGC 2 cut(s) 1044, 1136
Tsp45I GTSAC 3 cut(s) 14, 598, 850
TspDTI ATGAA 7 cut(s) 327, 390, 827, 911, 1098, 1160, 1188
TspGWI ACGGA 1 cut(s) 85
TspRI CASTG 7 cut(s) 289, 610, 772, 855, 901, 1000, 1045
Van91I CCANNNNNTGG 2 cut(s) 360, 1226
VpaK11BI GGWCC 4 cut(s) 65, 204, 351, 495
VspI ATTAAT 1 cut(s) 1095
XapI RAATTY 1 cut(s) 1123
XceI RCATGY 1 cut(s) 1147
XcmI CCANNNNNNNNNTGG 1 cut(s) 352
XmiI GTMKAC 1 cut(s) 609
XmnI GAANNNNTTC 1 cut(s) 471
XspI CTAG 2 cut(s) 860, 929
ZrmI AGTACT 1 cut(s) 416
Zsp2I ATGCAT 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.