Rh1AG057200

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000356
Physical Location & Seq
Forward (+)
9333303 .. 9344412
11110 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG057200.1

Sequence Viewer

Length: 2211 bp
ATGATGTACCCTGATCCTCAACAGCAGCAGCAACACCAACAACAGCAGCAGCAGCAGCAACAACAACACTATCACCAGCAGCAGCAGCAACACCACCAGCACCAGCAGCAACACCACCAGCACCAGCAGCAGCAGCAGCAAGGTGGAGACTTTCACAGGGGCCCACCGCCGATGATGCGTCAGCCGTCTGCTTCTTCTACTAACATGGCTCCGGATTATCACCACCCCTCGGCCCCTGGTCCTCCTGTTCCTCCTTATGATGTTCATGGTGATGGTTTTGGTGCAAAGAGAAACAGAAAGCAGACTCAGAGAAGGGCGGTTGATTACACTAGCACTGTCGTGCGGTATACACAGACTAGAATGTGGCAGCGGGATGCGAGGGATAGGACAGTATTGCAACCTACACCAGCAGCAGCAATTGACATGTTGCCGACAGTCGCCTATTCAGATAACCCATCTACGGGCTTTGCTGCAAAGTTTGTGCATACTTCCACAAACAAAGTCCGTAATTCAATTAATCGGGTTTTGTGGACTCCTCCTGGGAGGCGTCTTATTACAGGCTCTCAAAATGGGGAATTCACTCTTTGGAATGGCCAATCATTTAATTTTGAAATGATTCTTCAGGCTCATGATCAAGCAATCAGATCTATGGTCTGGAGTCATAATGAAAATTGGATGGTCTCTGGTGATGATGGAGGATCAATAAAGTATTGGCAGACCAACATGAATAATGTGAAGGCCAATAAATCTGCTCACAAAGAATCTGTTCAGGACTTAAGCTTCTGTAAGACTGACTTGAAGTTCTGTTCCTGTTCGAATGATACTTCTGTTAAAATATGGGATTTTGCACGGTGCCAAGAAGAGCGCACATTGACTGGCCATGGTTGGGATGTGAAGAGTGTTGACTGGCACCCTACAAAGTCTCTACTAGCTTCAGGTGGGAAAGACCAGCTTGTCAAACTTTGGGACGCTAAGTCAGGGCGAGAACTATGTTCATTTCATGGTCACAAAAATTTGGTGCAATCTGTTAAATGGAACCAAAATGGCAACTGGCTGCTAACTGCTTCTAAGGATCAAGTCATAAAGCTTTACGACATAAGGGCTATGAAGGAGCTTGAATCTTTCCGTGGGCATCGGAAGGAAGTGACTGCTCTGGCTTGGCATCCTTTTCATGAAGAATATTTTGTCAGTGGGAGTTCTGATGGATCCATTTTCCATTGGCTTGTTGGGCATGAGACTCCCCAGGTTGAAATTGTTAATGCACACGATAACAGTGTGTGGGATCTCCAATGGCACCCTATTGGCTATATTCTTTGCAGTGGTAGCAATGATCACACAACAAAGTTTTGGTGCAGAAACAGGCCAGGAGACACTGGTCGCGATAAATTTAACATCAATCAGAGTCAAGGTTATGGTGATCAAAACTCTGCTTTTGCTGGTCGCATGACGGGTAATTTTCCATTACACGATGGACCACCAACTCCAGGACCATTTACTCCCGGGGGAACTCGAAATGAAGGAAACATTCCAGGGGTTGGAGTAGCTATGCCGTTAGCCATACCATCCCTTGACGCATCTATTCAGGGAGAACATCAGCAACCTCATGCACATTCAATGCCTTTAGGAGCTCCTCCTCTCCCACCTGGTCCACATCCTTCTCTTCTTGCTGCAAATCAGCAGCAACCATATCATCAAAATGCGCAACAGCAACATCAGCAACACCAAGGTCACCCACAACAAATGCCCCCTTTGCCTATGCCACCTCCAAATATGCCACAGCTACAGCCTCCATCCCATTTACCCTTGCTGCCGCATCCTCATTTACGTCCACCACCCCAAATGCCCCCACTCGGTATGCCATCTTCAGTGTCCGGATCTTTGCCTATGCCTTCATCAGTTCCAACATCACTTTCAATGCCAATGGGCATGCAAGGTACAATGAATCAGATGGTTCATCAATTGCCACAAGGTCATTATATGGGCATGAACCCACCTCCACCTGCTGGAGGTTTCCCAAATGGGATGCCGAACATGCAGGGCCCATCTAATGCAAGTGGAGGGCAAATGTATTCTCATGCTGGCACCTTCAATCGTGCACAATCTGGACAAATGCCAATGCTACCGGGGTATAATCCCTACCAGCCCAGGGGTCAATCCGGTATGCCTCAGCCTCCACCAGGGCCACCACCACATGGGCAAACACCTCAATAG

Protein Analysis

736

Amino Acids

82.14

Weight (kDa)

8.79

Isoelectric Point (pI)

55.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_MABP1-WDR62_2nd PF24782 120 - 407 2e-14 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 154 - 332 2.4e-25 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 162 - 240 2.7e-06 WDR3 second beta-propeller domain
WDR55 PF24796 164 - 241 8.3e-06 WDR55
Beta-prop_WDR3_1st PF25173 165 - 317 6.5e-23 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 167 - 327 8.6e-26 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 167 - 340 9.2e-27 THOC3 beta-propeller domain
Beta-prop_IFT140_1st PF23383 171 - 257 2.6e-09 IFT140 first beta-propeller
WD40_Gbeta PF25391 172 - 375 4.5e-20 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 176 - 333 3.9e-15 WDHD1 first WD40 domain
Beta-prop_EML PF23409 181 - 247 1.6e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 181 - 374 2.3e-31 Prp19 WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 203 - 318 2.6e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 205 - 238 1e-05 WD domain, G-beta repeat
EIF3I PF24805 206 - 288 1.5e-08 EIF3I
Beta-prop_TEP1_2nd PF25047 215 - 385 1.3e-14 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 234 - 365 1.3e-28 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 234 - 324 6.7e-10 WDR3 second beta-propeller domain
WDR55 PF24796 243 - 406 5.5e-16 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 247 - 407 1.9e-18 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 263 - 407 4.8e-19 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 267 - 355 5.6e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_1st PF25173 279 - 407 7.1e-29 WDR3 first beta-propeller domain
Beta-prop_IFT140_1st PF23383 286 - 361 4.8e-06 IFT140 first beta-propeller
WD40 PF00400 287 - 323 4.3e-09 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 289 - 371 1.2e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 290 - 410 6.3e-14 WDR3 second beta-propeller domain
EIF3I PF24805 290 - 449 6.2e-14 EIF3I
Beta-prop_EML PF23409 291 - 397 5.3e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin PF25460 297 - 406 1.6e-06 Aladin seven-bladed propeller
Beta-prop_WDR19_1st PF23389 301 - 448 3.6e-08 WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 318 - 451 2.3e-10 WDR36/Utp21 first beta-propeller
WD40 PF00400 328 - 365 5.8e-08 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 328 - 452 2.3e-22 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 334 - 451 6.4e-21 WDR5 beta-propeller domain
ANAPC4_WD40 PF12894 335 - 388 8.7e-06 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_THOC3 PF25174 336 - 452 1.5e-22 THOC3 beta-propeller domain
Beta-prop_EIPR1 PF23609 336 - 404 2.1e-06 EIPR1 beta-propeller
WD40_WDHD1_1st PF24817 340 - 420 2.1e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 372 - 450 3.1e-10 Prp19 WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 373 - 449 9.7e-06 CAF1B/HIR1 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica Prupe.2G034300_v2.0.a1
pyrus_communis pycom02g24420 pycom07g02650
rosa_chinensis RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2008
AasI GACNNNNNNGTC 1 cut(s) 953
Acc16I TGCGCA 1 cut(s) 1701
Acc36I ACCTGC 1 cut(s) 2008
AccB1I GGYRCC 4 cut(s) 852, 909, 1293, 2081
AccB7I CCANNNNNTGG 3 cut(s) 1535, 2003, 2192
AccI GTMKAC 1 cut(s) 347
AccII CGCG 1 cut(s) 1380
AccIII TCCGGA 2 cut(s) 211, 1871
AciI CCGC 5 cut(s) 167, 317, 343, 370, 1811
AclWI GGATC 7 cut(s) 8, 706, 1080, 1200, 1213, 1290, 1882
AcoI YGGCCR 2 cut(s) 592, 877
AcsI RAATTY 3 cut(s) 575, 1012, 1385
AcuI CTGAAG 3 cut(s) 605, 918, 1848
AcyI GRCGYC 1 cut(s) 547
AfaI GTAC 2 cut(s) 8, 1936
AflII CTTAAG 1 cut(s) 775
AflIII ACRYGT 1 cut(s) 423
AgsI TTSAA 8 cut(s) 513, 611, 799, 1118, 1250, 1614, 1914, 2089
AhdI GACNNNNNGTC 1 cut(s) 973
AjnI CCWGG 9 cut(s) 235, 538, 1242, 1363, 1483, 1528, 1642, 2144, 2176
AjuI GAANNNNNNNTTGG 2 cut(s) 1894, 1926
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 8 cut(s) 780, 932, 952, 1087, 1114, 1544, 1628, 1780
AluI AGCT 8 cut(s) 780, 932, 952, 1087, 1114, 1544, 1628, 1780
Alw21I GWGCWC 2 cut(s) 1630, 2098
Alw26I GTCTC 5 cut(s) 141, 685, 927, 1229, 1362
Alw44I GTGCAC 1 cut(s) 2094
AlwI GGATC 7 cut(s) 8, 706, 1080, 1200, 1213, 1290, 1882
Ama87I CYCGRG 1 cut(s) 1499
Aor13HI TCCGGA 2 cut(s) 211, 1871
AoxI GGCC 8 cut(s) 160, 231, 592, 738, 877, 1361, 2038, 2180
ApaI GGGCCC 2 cut(s) 164, 2042
ApaLI GTGCAC 1 cut(s) 2094
ApoI RAATTY 3 cut(s) 575, 1012, 1385
AseI ATTAAT 1 cut(s) 516
Asp700I GAANNNNTTC 2 cut(s) 615, 765
AspLEI GCGC 2 cut(s) 867, 1702
AsuC2I CCSGG 3 cut(s) 1500, 1501, 2124
AsuHPI GGTGA 6 cut(s) 65, 212, 281, 698, 1427, 1721
AsuII TTCGAA 1 cut(s) 815
AvaI CYCGRG 1 cut(s) 1499
AvaII GGWCC 4 cut(s) 239, 1472, 1487, 1646
BaeGI GKGCMC 3 cut(s) 164, 2042, 2098
BaeI ACNNNNGTAYC 2 cut(s) 813, 846
BalI TGGCCA 2 cut(s) 594, 879
BamHI GGATCC 1 cut(s) 1205
BanI GGYRCC 4 cut(s) 852, 909, 1293, 2081
BanII GRGCYC 3 cut(s) 164, 1630, 2042
BarI GAAGNNNNNNTAC 2 cut(s) 1846, 1878
Bbv12I GWGCWC 2 cut(s) 1630, 2098
BbvCI CCTCAGC 1 cut(s) 2166
BceAI ACGGC 2 cut(s) 169, 1534
BciT130I CCWGG 9 cut(s) 237, 540, 1244, 1365, 1485, 1530, 1644, 2146, 2178
BclI TGATCA 3 cut(s) 631, 1330, 1417
BcnI CCSGG 3 cut(s) 1500, 1501, 2124
BcoDI GTCTC 5 cut(s) 141, 685, 927, 1229, 1362
BfaI CTAG 3 cut(s) 330, 357, 929
BfmI CTRYAG 1 cut(s) 1781
BfrI CTTAAG 1 cut(s) 775
BfuAI ACCTGC 1 cut(s) 2008
BglII AGATCT 1 cut(s) 644
Bme18I GGWCC 4 cut(s) 239, 1472, 1487, 1646
BmeRI GACNNNNNGTC 1 cut(s) 973
BmeT110I CYCGRG 1 cut(s) 1499
BmsI GCATC 7 cut(s) 165, 364, 1141, 1171, 1583, 1822, 2013
BoxI GACNNNNGTC 1 cut(s) 1374
BpmI CTGGAG 3 cut(s) 676, 1467, 2025
Bpu10I CCTNAGC 1 cut(s) 2166
Bpu14I TTCGAA 1 cut(s) 815
BpuMI CCSGG 3 cut(s) 1500, 1501, 2124
BsaHI GRCGYC 1 cut(s) 547
BsaI GGTCTC 1 cut(s) 685
BsaWI WCCGGW 3 cut(s) 211, 1871, 2156
Bse1I ACTGG 4 cut(s) 880, 911, 1055, 1378
Bse3DI GCAATG 1 cut(s) 1333
BseAI TCCGGA 2 cut(s) 211, 1871
BseBI CCWGG 9 cut(s) 237, 540, 1244, 1365, 1485, 1530, 1644, 2146, 2178
BseGI GGATG 9 cut(s) 379, 681, 895, 1162, 1562, 1651, 1790, 1813, 2028
BseMI GCAATG 1 cut(s) 1333
BseMII CTCAG 2 cut(s) 320, 2180
BseNI ACTGG 4 cut(s) 880, 911, 1055, 1378
BseRI GAGGAG 3 cut(s) 525, 1620, 1623
BseSI GKGCMC 3 cut(s) 164, 2042, 2098
BsgI GTGCAG 1 cut(s) 1372
Bsh1236I CGCG 1 cut(s) 1380
BshFI GGCC 8 cut(s) 162, 233, 594, 740, 879, 1363, 2040, 2182
BshNI GGYRCC 4 cut(s) 852, 909, 1293, 2081
BsiHKAI GWGCWC 2 cut(s) 1630, 2098
BsiHKCI CYCGRG 1 cut(s) 1499
BsiSI CCGG 5 cut(s) 212, 1500, 1872, 2123, 2157
BslFI GGGAC 1 cut(s) 980
BsmAI GTCTC 5 cut(s) 141, 685, 927, 1229, 1362
BsmFI GGGAC 1 cut(s) 980
BsnI GGCC 8 cut(s) 162, 233, 594, 740, 879, 1363, 2040, 2182
Bso31I GGTCTC 1 cut(s) 685
BsoBI CYCGRG 1 cut(s) 1499
Bsp119I TTCGAA 1 cut(s) 815
Bsp120I GGGCCC 2 cut(s) 160, 2038
Bsp1286I GDGCHC 4 cut(s) 164, 1630, 2042, 2098
Bsp13I TCCGGA 2 cut(s) 211, 1871
Bsp19I CCATGG 1 cut(s) 880
Bsp68I TCGCGA 1 cut(s) 1380
BspACI CCGC 5 cut(s) 167, 317, 343, 370, 1811
BspANI GGCC 8 cut(s) 162, 233, 594, 740, 879, 1363, 2040, 2182
BspCNI CTCAG 2 cut(s) 319, 2179
BspEI TCCGGA 2 cut(s) 211, 1871
BspFNI CGCG 1 cut(s) 1380
BspHI TCATGA 2 cut(s) 628, 1171
BspMI ACCTGC 1 cut(s) 2008
BspPI GGATC 7 cut(s) 8, 706, 1080, 1200, 1213, 1290, 1882
BspQI GCTCTTC 1 cut(s) 855
BspT104I TTCGAA 1 cut(s) 815
BspT107I GGYRCC 4 cut(s) 852, 909, 1293, 2081
BspTI CTTAAG 1 cut(s) 775
BspTNI GGTCTC 1 cut(s) 685
BsrDI GCAATG 1 cut(s) 1333
BsrI ACTGG 4 cut(s) 880, 911, 1055, 1378
BssNAI GTATAC 1 cut(s) 348
BssNI GRCGYC 1 cut(s) 547
BssT1I CCWWGG 2 cut(s) 880, 1723
Bst1107I GTATAC 1 cut(s) 348
Bst2UI CCWGG 9 cut(s) 237, 540, 1244, 1365, 1485, 1530, 1644, 2146, 2178
Bst4CI ACNGT 5 cut(s) 337, 391, 436, 852, 1274
Bst6I CTCTTC 3 cut(s) 855, 890, 1665
BstACI GRCGYC 1 cut(s) 547
BstAFI CTTAAG 1 cut(s) 775
BstBI TTCGAA 1 cut(s) 815
BstC8I GCNNGC 2 cut(s) 1928, 2080
BstDEI CTNAG 4 cut(s) 306, 972, 1068, 2166
BstDSI CCRYGG 2 cut(s) 880, 1126
BstEII GGTNACC 1 cut(s) 1727
BstENI CCTNNNNNAGG 2 cut(s) 2004, 2175
BstF5I GGATG 9 cut(s) 379, 681, 895, 1162, 1562, 1651, 1790, 1813, 2028
BstFNI CGCG 1 cut(s) 1380
BstHHI GCGC 2 cut(s) 867, 1702
BstMAI GTCTC 5 cut(s) 141, 685, 927, 1229, 1362
BstNI CCWGG 9 cut(s) 237, 540, 1244, 1365, 1485, 1530, 1644, 2146, 2178
BstNSI RCATGY 3 cut(s) 427, 1930, 2035
BstPAI GACNNNNGTC 1 cut(s) 1374
BstPI GGTNACC 1 cut(s) 1727
BstSFI CTRYAG 1 cut(s) 1781
BstSLI GKGCMC 3 cut(s) 164, 2042, 2098
BstUI CGCG 1 cut(s) 1380
BstX2I RGATCY 4 cut(s) 644, 1205, 1282, 1874
BstYI RGATCY 4 cut(s) 644, 1205, 1282, 1874
BstZ17I GTATAC 1 cut(s) 348
BsuRI GGCC 8 cut(s) 162, 233, 594, 740, 879, 1363, 2040, 2182
BtgI CCRYGG 2 cut(s) 880, 1126
BtsCI GGATG 9 cut(s) 379, 681, 895, 1162, 1562, 1651, 1790, 1813, 2028
BtsI GCAGTG 1 cut(s) 1324
BtsIMutI CAGTG 6 cut(s) 333, 1195, 1279, 1324, 1371, 1872
BtuMI TCGCGA 1 cut(s) 1380
BveI ACCTGC 1 cut(s) 2008
Cac8I GCNNGC 2 cut(s) 1928, 2080
CciI TCATGA 2 cut(s) 628, 1171
CfoI GCGC 2 cut(s) 867, 1702
Cfr9I CCCGGG 1 cut(s) 1499
CseI GACGC 4 cut(s) 167, 536, 977, 1580
CsiI ACCWGGT 1 cut(s) 1642
Csp6I GTAC 2 cut(s) 7, 1935
CviQI GTAC 2 cut(s) 7, 1935
DdeI CTNAG 4 cut(s) 306, 972, 1068, 2166
DrdI GACNNNNNNGTC 1 cut(s) 953
DriI GACNNNNNGTC 1 cut(s) 973
DseDI GACNNNNNNGTC 1 cut(s) 953
EaeI YGGCCR 2 cut(s) 592, 877
Eam1104I CTCTTC 3 cut(s) 855, 890, 1665
Eam1105I GACNNNNNGTC 1 cut(s) 973
EarI CTCTTC 3 cut(s) 855, 890, 1665
Ecl136II GAGCTC 1 cut(s) 1628
Eco130I CCWWGG 2 cut(s) 880, 1723
Eco24I GRGCYC 3 cut(s) 164, 1630, 2042
Eco31I GGTCTC 1 cut(s) 685
Eco47I GGWCC 4 cut(s) 239, 1472, 1487, 1646
Eco53kI GAGCTC 1 cut(s) 1628
Eco57I CTGAAG 3 cut(s) 605, 918, 1848
Eco88I CYCGRG 1 cut(s) 1499
Eco91I GGTNACC 1 cut(s) 1727
EcoICRI GAGCTC 1 cut(s) 1628
EcoNI CCTNNNNNAGG 2 cut(s) 2004, 2175
EcoO109I RGGNCCY 2 cut(s) 160, 2038
EcoO65I GGTNACC 1 cut(s) 1727
EcoRI GAATTC 1 cut(s) 575
EcoRII CCWGG 9 cut(s) 235, 538, 1242, 1363, 1483, 1528, 1642, 2144, 2176
EcoT14I CCWWGG 2 cut(s) 880, 1723
EcoT38I GRGCYC 3 cut(s) 164, 1630, 2042
ErhI CCWWGG 2 cut(s) 880, 1723
FalI AAGNNNNNCTT 4 cut(s) 779, 811, 936, 968
FaqI GGGAC 1 cut(s) 980
FauI CCCGC 1 cut(s) 363
FbaI TGATCA 3 cut(s) 631, 1330, 1417
FblI GTMKAC 1 cut(s) 347
FokI GGATG 9 cut(s) 386, 688, 902, 1149, 1549, 1638, 1777, 1800, 2035
FriOI GRGCYC 3 cut(s) 164, 1630, 2042
FspBI CTAG 3 cut(s) 330, 357, 929
FspI TGCGCA 1 cut(s) 1701
GlaI GCGC 2 cut(s) 866, 1701
GsuI CTGGAG 3 cut(s) 676, 1467, 2025
HaeIII GGCC 8 cut(s) 162, 233, 594, 740, 879, 1363, 2040, 2182
HapII CCGG 5 cut(s) 212, 1500, 1872, 2123, 2157
HgaI GACGC 4 cut(s) 167, 536, 977, 1580
HhaI GCGC 2 cut(s) 867, 1702
Hin1I GRCGYC 1 cut(s) 547
Hin6I GCGC 2 cut(s) 865, 1700
HinP1I GCGC 2 cut(s) 865, 1700
HincII GTYRAC 1 cut(s) 904
HindII GTYRAC 1 cut(s) 904
HindIII AAGCTT 2 cut(s) 778, 1085
HinfI GANTC 9 cut(s) 304, 532, 616, 658, 761, 1118, 1237, 1402, 1942
HpaII CCGG 5 cut(s) 212, 1500, 1872, 2123, 2157
HphI GGTGA 6 cut(s) 65, 212, 281, 698, 1427, 1721
Hpy166II GTNNAC 6 cut(s) 348, 531, 904, 1649, 1829, 2096
Hpy188I TCNGA 7 cut(s) 309, 448, 644, 1137, 1201, 1401, 1947
Hpy188III TCNNGA 8 cut(s) 212, 629, 655, 770, 1172, 1379, 1872, 2103
Hpy8I GTNNAC 6 cut(s) 348, 531, 904, 1649, 1829, 2096
HpyAV CCTTC 8 cut(s) 306, 730, 1102, 1132, 1511, 1665, 1899, 2095
HpyCH4III ACNGT 5 cut(s) 337, 391, 436, 852, 1274
HpyCH4IV ACGT 1 cut(s) 1825
HpyF3I CTNAG 4 cut(s) 306, 972, 1068, 2166
HpySE526I ACGT 1 cut(s) 1825
Hsp92I GRCGYC 1 cut(s) 547
HspAI GCGC 2 cut(s) 865, 1700
Kpn2I TCCGGA 2 cut(s) 211, 1871
Ksp22I TGATCA 3 cut(s) 631, 1330, 1417
LguI GCTCTTC 1 cut(s) 855
LmnI GCTCC 4 cut(s) 214, 1111, 1625, 1633
LweI GCATC 7 cut(s) 165, 364, 1141, 1171, 1583, 1822, 2013
MabI ACCWGGT 1 cut(s) 1642
MaeI CTAG 3 cut(s) 330, 357, 929
MaeII ACGT 1 cut(s) 1825
MaeIII GTNAC 3 cut(s) 1004, 1144, 1727
MboII GAAGA 7 cut(s) 186, 611, 872, 907, 1187, 1652, 1854
MfeI CAATTG 2 cut(s) 417, 1958
MflI RGATCY 4 cut(s) 644, 1205, 1282, 1874
MhlI GDGCHC 4 cut(s) 164, 1630, 2042, 2098
MlsI TGGCCA 2 cut(s) 594, 879
MluNI TGGCCA 2 cut(s) 594, 879
MlyI GAGTC 5 cut(s) 298, 526, 667, 1231, 1411
MmeI TCCRAC 2 cut(s) 1516, 1925
Mox20I TGGCCA 2 cut(s) 594, 879
MroI TCCGGA 2 cut(s) 211, 1871
MroXI GAANNNNTTC 2 cut(s) 615, 765
MscI TGGCCA 2 cut(s) 594, 879
MseI TTAA 7 cut(s) 516, 603, 776, 831, 1029, 1257, 1389
MslI CAYNNNNRTG 3 cut(s) 270, 338, 1695
Msp20I TGGCCA 2 cut(s) 594, 879
MspA1I CMGCKG 1 cut(s) 370
MspCI CTTAAG 1 cut(s) 775
MspI CCGG 5 cut(s) 212, 1500, 1872, 2123, 2157
MunI CAATTG 2 cut(s) 417, 1958
MvaI CCWGG 9 cut(s) 237, 540, 1244, 1365, 1485, 1530, 1644, 2146, 2178
MvnI CGCG 1 cut(s) 1380
NciI CCSGG 3 cut(s) 1500, 1501, 2124
NcoI CCATGG 1 cut(s) 880
NmeAIII GCCGAG 1 cut(s) 209
NmuCI GTSAC 3 cut(s) 1004, 1144, 1727
NruI TCGCGA 1 cut(s) 1380
NsbI TGCGCA 1 cut(s) 1701
NspI RCATGY 3 cut(s) 427, 1930, 2035
NspV TTCGAA 1 cut(s) 815
OliI CACNNNNGTG 1 cut(s) 338
PaeI GCATGC 1 cut(s) 1930
PagI TCATGA 2 cut(s) 628, 1171
PaqCI CACCTGC 1 cut(s) 2008
PasI CCCWGGG 1 cut(s) 2145
PciI ACATGT 1 cut(s) 423
PciSI GCTCTTC 1 cut(s) 855
PdmI GAANNNNTTC 2 cut(s) 615, 765
PfeI GAWTC 4 cut(s) 616, 761, 1118, 1942
PflMI CCANNNNNTGG 3 cut(s) 1535, 2003, 2192
PfoI TCCNGGA 1 cut(s) 1483
PleI GAGTC 5 cut(s) 298, 526, 666, 1231, 1410
PpsI GAGTC 5 cut(s) 298, 526, 666, 1231, 1410
PscI ACATGT 1 cut(s) 423
PshAI GACNNNNGTC 1 cut(s) 1374
PshBI ATTAAT 1 cut(s) 516
Psp124BI GAGCTC 1 cut(s) 1630
Psp6I CCWGG 9 cut(s) 235, 538, 1242, 1363, 1483, 1528, 1642, 2144, 2176
PspEI GGTNACC 1 cut(s) 1727
PspGI CCWGG 9 cut(s) 235, 538, 1242, 1363, 1483, 1528, 1642, 2144, 2176
PspOMI GGGCCC 2 cut(s) 160, 2038
PsuI RGATCY 4 cut(s) 644, 1205, 1282, 1874
RruI TCGCGA 1 cut(s) 1380
RsaI GTAC 2 cut(s) 8, 1936
RsaNI GTAC 2 cut(s) 7, 1935
RseI CAYNNNNRTG 3 cut(s) 270, 338, 1695
SacI GAGCTC 1 cut(s) 1630
SapI GCTCTTC 1 cut(s) 855
SaqAI TTAA 7 cut(s) 516, 603, 776, 831, 1029, 1257, 1389
SchI GAGTC 5 cut(s) 298, 526, 667, 1231, 1411
SduI GDGCHC 4 cut(s) 164, 1630, 2042, 2098
SexAI ACCWGGT 1 cut(s) 1642
SfaNI GCATC 7 cut(s) 165, 364, 1141, 1171, 1583, 1822, 2013
SfcI CTRYAG 1 cut(s) 1781
SfuI TTCGAA 1 cut(s) 815
SinI GGWCC 4 cut(s) 239, 1472, 1487, 1646
SmaI CCCGGG 1 cut(s) 1501
SmiMI CAYNNNNRTG 3 cut(s) 270, 338, 1695
SmlI CTYRAG 1 cut(s) 775
SmoI CTYRAG 1 cut(s) 775
SphI GCATGC 1 cut(s) 1930
SsiI CCGC 5 cut(s) 167, 317, 343, 370, 1811
SspI AATATT 1 cut(s) 1181
SspMI CTAG 3 cut(s) 330, 357, 929
SstI GAGCTC 1 cut(s) 1630
StyI CCWWGG 2 cut(s) 880, 1723
TaaI ACNGT 5 cut(s) 337, 391, 436, 852, 1274
TaiI ACGT 1 cut(s) 1828
TaqI TCGA 2 cut(s) 815, 1510
TauI GCSGC 1 cut(s) 1813
TfiI GAWTC 4 cut(s) 616, 761, 1118, 1942
Tru1I TTAA 7 cut(s) 516, 603, 776, 831, 1029, 1257, 1389
Tru9I TTAA 7 cut(s) 516, 603, 776, 831, 1029, 1257, 1389
TscAI CASTG 6 cut(s) 340, 1195, 1279, 1324, 1378, 1872
TseFI GTSAC 3 cut(s) 1004, 1144, 1727
Tsp45I GTSAC 3 cut(s) 1004, 1144, 1727
TspGWI ACGGA 2 cut(s) 494, 1115
TspMI CCCGGG 1 cut(s) 1499
TspRI CASTG 6 cut(s) 340, 1195, 1279, 1324, 1378, 1872
Van91I CCANNNNNTGG 3 cut(s) 1535, 2003, 2192
Vha464I CTTAAG 1 cut(s) 775
VneI GTGCAC 1 cut(s) 2094
VpaK11BI GGWCC 4 cut(s) 239, 1472, 1487, 1646
VspI ATTAAT 1 cut(s) 516
XagI CCTNNNNNAGG 2 cut(s) 2004, 2175
XapI RAATTY 3 cut(s) 575, 1012, 1385
XceI RCATGY 3 cut(s) 427, 1930, 2035
XcmI CCANNNNNNNNNTGG 1 cut(s) 1223
XmaI CCCGGG 1 cut(s) 1499
XmiI GTMKAC 1 cut(s) 347
XmnI GAANNNNTTC 2 cut(s) 615, 765
XspI CTAG 3 cut(s) 330, 357, 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.