Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0000356

Physical Location & Seq

Genomic Coordinates Forward (+)

3134922 .. 3145176

Gene Structure 10255 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1038200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2235 bp

ATGATGTACCCTGATCCACAACAGCAACACCACCAACAGCAGCTTCAGCAGCAGCACCCGCAACATCAGCAGCAGCAGCATCCCCAACAACACCAGCAATATCAGCAGCAACATCCGCAGCAACATCAGCAGCAAGGTGGAGAATTTCATAGGGGGCCGCCGCCACCGGCCCCAATGATGCGTCAGCCGTCAGCCTCTTCAACTAATATGCCTCCAGAGTATCACAACCAACCTGGGCTCGCTCCTCCTCTTCCTCCTTATGATGCTCATGGTGACAGTTTTCCTGGAAAAAGGATGAGAAAGCTTACACACAGGAGGGCAGTTGATTATACTAGCACCGTTGTGCGATATATGCAGATTCGAATGTGGCAGCGCGATGCAAGAGATAGAACGGTATTGCAACCCACACCTGCAGCAGCAATTGATATGTTGCCTCCAGTTGCCTACTCAGATAATCCATCCACTAGCTTTGCTGCAAAGTTTGTACACACTTCTTTAAACAAGAATCGTTGTTCGATTAATCGGGTTTTGTGGACTCCTACAGGAAGGCGTCTTATTACTGGCTCTCAAAGTGGGGAATTCACTCTTTGGAATGGGCAATCATTTAACTTTGAAATGATTCTTCAGGCTCATGATCAAGCAATCAGGTCTATGGTATGGAGTCATAATGACAATTGGATGGTATCTGGTGATGATGGCGGTGCAATAAAGTACTGGCAGAACAATATGAATAATGTAAAGGCCAATAAATCTGCTCATAAAGAATCTGTTCGTGACTTAAGCTTCTGCAAGACTGACTTGAAGTTCTGTTCCTGCTCTGATGATAATACTGTTAAAGTTTGGGACTTTGCACGGTGCCAAGAAGAGCGCACATTAACTGGCCATGGTTGGGATGTGAAGAGTGTTGACTGGCACCCTACTAAATCCCTACTAGTTTCAGGTGGGAAAGATAATCTTGTTAAACTTTGGGATGCTAAGTCAGGGCGAGAACTCTGTTCATTTCATGGCCATAAAAATATGGTGCTCTCAGTTAAATGGAACCAAAATGGTAACTGGGTGCTAACTGCTTCGAAGGATCAAATCATCAAGGTTTATGACATAAGGGCCATGAAGGAACTTGCATCCTTCCGTGGGCATCGAAAGGATGTGACCGCTTTGGCTTGGCATCCGTTTCATGAAGAATATTTTGTCAGTGGGAGTTCTGATGGATCCATTTACCATTGGCTTGTTGGGCATGAAACTCCCCAGGTTGAAATTCCTAATGCACATGATCACAGTGTGTGGGATCTTGCGTGGCATCCTATTGGTTACCTTCTCTGCAGTGGTAGCAATGATCACACTACGAGGTTTTGGTGCAGAAATAGGCCAGGAGACACTGGTCGGGATAAATTTAACCCCGGCCAAAATCAAGGTTATGGTGAGCAAAACCCTGCTTTTGGTGGTCGCATGACTGGTAATTTTCCAGTACCTGAAGGACCACCAACTCCTGGACCACTTGTTCCTGGGTTGACTCGAAATGAAGGAACTATTCCAGGTGTTGGAGTTGCTATGCCATTCTCTCTTGATGCATCAAATCAGGGGGAGCAACAGCAACCTCATGCACTTTCAATGCCTTTAGGTGCTCCTCCTCTCCCGCCTGGTCCCCACCCCTCTCTTCTTGCTGCGAATCAGCAGCAACAATATCAGCAAAATCCTCAACAGATGCCACAACAGCAACACCAACACCAAGGTCACCCGCAACAAATGCCCCCTATGCCTATGGCACCTCCAAATATGCCACAGCTGCAGCCTCCATCCCATCTACCCTTGCTGCCGCATCCTCATTTACATCGACCGCCACCCCAAATGCCCCCACTTGGTATGCCATCTTCAGGGCCAGGATCTTTGTCTATGCCTTCATCGGTTCCTACCTCACATTCAATGCCAATGGGAATTCAAGGTACAATGAATCAGATGGGTCACCCATTGCCACAAGGTCATTTTATGGGCATGAACCCAATGCACTCGGGATCTCTACCTACAAGCGGTGGAGGTTTTCCAAATGGTATGCCAAACATGCAGGGCCCAACAAATGCAAGTGGACCTCAAATGTATCCTCAGGGTGGCACCTTCAACCGGGGTCAACCTGGGCAAATTCCCATGCATCCGGGTTATAATCCTTACCAGTCTAGGGGTCAATCTGGCATGCCCCAACCTCCACCAGGGCCACCACCGCACGGGCAAACACCTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

WD40

Protein Analysis

745

Amino Acids

82.89

Weight (kDa)

8.86

Isoelectric Point (pI)

54.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_MABP1-WDR62_2nd	PF24782	120 - 408	1.4e-14	MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd	PF25172	163 - 242	3.5e-06	WDR3 second beta-propeller domain
WD40_CDC20-Fz	PF24807	164 - 333	7.3e-24	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	164 - 341	1.1e-25	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	165 - 318	4.9e-22	WDR3 first beta-propeller domain
Beta-prop_EIPR1	PF23609	165 - 323	6.6e-06	EIPR1 beta-propeller
Beta-prop_IFT140_1st	PF23383	166 - 254	3e-10	IFT140 first beta-propeller
Beta-prop_WDR5	PF25175	167 - 243	2.4e-07	WDR5 beta-propeller domain
WD40_Gbeta	PF25391	171 - 376	1.3e-18	G protein beta WD-40 repeat protein
Beta-prop_EML	PF23409	178 - 248	2.9e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	206 - 307	1.5e-07	CAF1B/HIR1 beta-propeller domain
EIF3I	PF24805	207 - 287	4.4e-08	EIF3I
WD40_Prp19	PF24814	223 - 372	4.8e-23	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	235 - 347	5.3e-23	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	236 - 325	1.2e-09	WDR3 second beta-propeller domain
WDR55	PF24796	244 - 427	2.9e-17	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	248 - 409	4.3e-17	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	251 - 282	4.8e-06	WD domain, G-beta repeat
Beta-prop_EML_2	PF23414	264 - 410	1.4e-18	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	268 - 356	1.9e-06	WDR90/POC16, second beta-propeller
Beta-prop_WDR3_1st	PF25173	287 - 408	5e-26	WDR3 first beta-propeller domain
WD40	PF00400	288 - 324	2.3e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	290 - 372	4.6e-12	CAF1B/HIR1 beta-propeller domain
EIF3I	PF24805	291 - 450	1.9e-11	EIF3I
Beta-prop_WDR3_2nd	PF25172	292 - 411	6.5e-14	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	292 - 398	1.2e-08	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin	PF25460	298 - 409	5.6e-07	Aladin seven-bladed propeller
Beta-prop_TEP1_2nd	PF25047	299 - 385	2.4e-09	TEP-1 second beta-propeller
Beta-prop_WDR19_1st	PF23389	302 - 448	8.5e-06	WDR19 first beta-propeller
Beta-prop_WDR3_1st	PF25173	314 - 451	8.8e-26	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	315 - 452	6.7e-10	WDR36/Utp21 first beta-propeller
Beta-prop_EML	PF23409	322 - 452	1.2e-09	Echinoderm microtubule-associated protein first beta-propeller
WD40_CDC20-Fz	PF24807	329 - 453	2e-21	CDC20/Fizzy WD40 domain
WD40	PF00400	329 - 366	2.9e-08	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	332 - 453	1.1e-10	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	335 - 452	1.7e-20	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	337 - 452	1.5e-21	THOC3 beta-propeller domain
Beta-prop_EIPR1	PF23609	337 - 406	1e-05	EIPR1 beta-propeller
WD40_Prp19	PF24814	341 - 451	2.1e-17	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	341 - 435	9.2e-11	WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd	PF23393	354 - 444	9.9e-06	WDR90/POC16, second beta-propeller
WD40	PF00400	371 - 408	1.8e-06	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	375 - 451	2.4e-06	CAF1B/HIR1 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000356)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca	FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica	MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica	Prupe.2G034300_v2.0.a1
pyrus_communis	pycom02g24420 pycom07g02650
rosa_chinensis	RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata	RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora	Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii	Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa	Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha	Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana	Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2154
AarI	CACCTGC	1 cut(s)	418
Acc36I	ACCTGC	1 cut(s)	418
AccB1I	GGYRCC	4 cut(s)	855, 912, 1762, 2105
AccB7I	CCANNNNNTGG	2 cut(s)	1487, 1538
AccII	CGCG	1 cut(s)	375
AclWI	GGATC	7 cut(s)	8, 1083, 1203, 1216, 1293, 1888, 2017
AcoI	YGGCCR	3 cut(s)	880, 1006, 1399
AcsI	RAATTY	6 cut(s)	143, 578, 1254, 1388, 1932, 2133
AcuI	CTGAAG	4 cut(s)	29, 608, 1491, 1854
AcyI	GRCGYC	1 cut(s)	550
AdeI	CACNNNGTG	1 cut(s)	1279
AfaI	GTAC	5 cut(s)	8, 486, 713, 1467, 1942
AflII	CTTAAG	1 cut(s)	778
AgsI	TTSAA	8 cut(s)	201, 614, 802, 1253, 1608, 1920, 1937, 2113
AhlI	ACTAGT	1 cut(s)	931
AjuI	GAANNNNNNNTTGG	2 cut(s)	27, 59
AleI	CACNNNNGTG	1 cut(s)	341
AloI	GAACNNNNNNTCC	2 cut(s)	1482, 1514
AluBI	AGCT	5 cut(s)	43, 304, 468, 783, 1783
AluI	AGCT	5 cut(s)	43, 304, 468, 783, 1783
Alw21I	GWGCWC	2 cut(s)	1026, 1624
Alw26I	GTCTC	1 cut(s)	1365
AlwI	GGATC	7 cut(s)	8, 1083, 1203, 1216, 1293, 1888, 2017
AlwNI	CAGNNNCTG	1 cut(s)	1469
Ama87I	CYCGRG	1 cut(s)	2005
ApaI	GGGCCC	1 cut(s)	2066
ApoI	RAATTY	6 cut(s)	143, 578, 1254, 1388, 1932, 2133
AseI	ATTAAT	1 cut(s)	519
Asp700I	GAANNNNTTC	2 cut(s)	618, 768
AspLEI	GCGC	2 cut(s)	375, 870
AsuC2I	CCSGG	3 cut(s)	1398, 2117, 2148
AsuHPI	GGTGA	5 cut(s)	284, 701, 1430, 1724, 1952
AsuII	TTCGAA	2 cut(s)	361, 1070
AvaI	CYCGRG	1 cut(s)	2005
AvaII	GGWCC	4 cut(s)	1475, 1490, 1640, 2081
AxyI	CCTNAGG	1 cut(s)	2097
BaeGI	GKGCMC	1 cut(s)	2066
BalI	TGGCCA	2 cut(s)	882, 1008
BamHI	GGATCC	1 cut(s)	1208
BanI	GGYRCC	4 cut(s)	855, 912, 1762, 2105
BanII	GRGCYC	2 cut(s)	240, 2066
BarI	GAAGNNNNNNTAC	2 cut(s)	1852, 1884
Bbv12I	GWGCWC	2 cut(s)	1026, 1624
BccI	CCATC	8 cut(s)	466, 673, 689, 1199, 1801, 1806, 1873, 1948
BceAI	ACGGC	1 cut(s)	172
BciVI	GTATCC	1 cut(s)	2103
BclI	TGATCA	3 cut(s)	634, 1270, 1333
BcnI	CCSGG	3 cut(s)	1398, 2117, 2148
BcoDI	GTCTC	1 cut(s)	1365
BcuI	ACTAGT	1 cut(s)	931
BfaI	CTAG	4 cut(s)	333, 465, 932, 2169
BfmI	CTRYAG	4 cut(s)	411, 540, 1318, 1784
BfrI	CTTAAG	1 cut(s)	778
BfuAI	ACCTGC	1 cut(s)	418
BfuI	GTATCC	1 cut(s)	2103
BmcAI	AGTACT	1 cut(s)	713
Bme18I	GGWCC	4 cut(s)	1475, 1490, 1640, 2081
BmeT110I	CYCGRG	1 cut(s)	2005
BmrI	ACTGGG	1 cut(s)	1063
BmuI	ACTGGG	1 cut(s)	1063
BoxI	GACNNNNGTC	1 cut(s)	1377
BpmI	CTGGAG	2 cut(s)	198, 420
Bpu14I	TTCGAA	2 cut(s)	361, 1070
BpuMI	CCSGG	3 cut(s)	1398, 2117, 2148
BsaHI	GRCGYC	1 cut(s)	550
Bse118I	RCCGGY	1 cut(s)	166
Bse21I	CCTNAGG	1 cut(s)	2097
Bse3DI	GCAATG	2 cut(s)	1336, 1964
BseMI	GCAATG	2 cut(s)	1336, 1964
BseMII	CTCAG	3 cut(s)	462, 1041, 2111
BseRI	GAGGAG	4 cut(s)	234, 237, 1614, 1617
BseSI	GKGCMC	1 cut(s)	2066
BsgI	GTGCAG	1 cut(s)	1375
Bsh1236I	CGCG	1 cut(s)	375
Bsh1285I	CGRYCG	1 cut(s)	1835
BshNI	GGYRCC	4 cut(s)	855, 912, 1762, 2105
BsiEI	CGRYCG	1 cut(s)	1835
BsiHKAI	GWGCWC	2 cut(s)	1026, 1624
BsiHKCI	CYCGRG	1 cut(s)	2005
BsiSI	CCGG	4 cut(s)	167, 1398, 2116, 2147
BslFI	GGGAC	2 cut(s)	857, 1626
BsmAI	GTCTC	1 cut(s)	1365
BsmFI	GGGAC	2 cut(s)	857, 1626
BsoBI	CYCGRG	1 cut(s)	2005
Bsp119I	TTCGAA	2 cut(s)	361, 1070
Bsp120I	GGGCCC	1 cut(s)	2062
Bsp1286I	GDGCHC	4 cut(s)	240, 1026, 1624, 2066
Bsp1407I	TGTACA	1 cut(s)	484
Bsp143I	GATC	9 cut(s)	13, 634, 1075, 1208, 1270, 1285, 1333, 1880, 2009
Bsp19I	CCATGG	1 cut(s)	883
BspCNI	CTCAG	3 cut(s)	461, 1040, 2110
BspFNI	CGCG	1 cut(s)	375
BspHI	TCATGA	2 cut(s)	631, 1174
BspMAI	CTGCAG	3 cut(s)	415, 1322, 1788
BspMI	ACCTGC	1 cut(s)	418
BspPI	GGATC	7 cut(s)	8, 1083, 1203, 1216, 1293, 1888, 2017
BspQI	GCTCTTC	1 cut(s)	858
BspT104I	TTCGAA	2 cut(s)	361, 1070
BspT107I	GGYRCC	4 cut(s)	855, 912, 1762, 2105
BspTI	CTTAAG	1 cut(s)	778
BsrDI	GCAATG	2 cut(s)	1336, 1964
BsrFI	RCCGGY	1 cut(s)	166
BsrGI	TGTACA	1 cut(s)	484
BssAI	RCCGGY	1 cut(s)	166
BssMI	GATC	9 cut(s)	13, 634, 1075, 1208, 1270, 1285, 1333, 1880, 2009
BssNI	GRCGYC	1 cut(s)	550
BssT1I	CCWWGG	2 cut(s)	883, 1726
Bst4CI	ACNGT	6 cut(s)	278, 340, 394, 832, 855, 1277
Bst6I	CTCTTC	5 cut(s)	202, 255, 858, 893, 1659
BstACI	GRCGYC	1 cut(s)	550
BstAFI	CTTAAG	1 cut(s)	778
BstAPI	GCANNNNNTGC	1 cut(s)	1744
BstAUI	TGTACA	1 cut(s)	484
BstBI	TTCGAA	2 cut(s)	361, 1070
BstC8I	GCNNGC	2 cut(s)	240, 2186
BstDEI	CTNAG	5 cut(s)	448, 975, 1027, 2097, 2229
BstDSI	CCRYGG	2 cut(s)	883, 1129
BstEII	GGTNACC	3 cut(s)	1307, 1730, 1958
BstENI	CCTNNNNNAGG	1 cut(s)	2199
BstFNI	CGCG	1 cut(s)	375
BstHHI	GCGC	2 cut(s)	375, 870
BstKTI	GATC	9 cut(s)	16, 637, 1078, 1211, 1273, 1288, 1336, 1883, 2012
BstMAI	GTCTC	1 cut(s)	1365
BstMBI	GATC	9 cut(s)	13, 634, 1075, 1208, 1270, 1285, 1333, 1880, 2009
BstMCI	CGRYCG	1 cut(s)	1835
BstNSI	RCATGY	2 cut(s)	2059, 2188
BstPAI	GACNNNNGTC	1 cut(s)	1377
BstPI	GGTNACC	3 cut(s)	1307, 1730, 1958
BstSFI	CTRYAG	4 cut(s)	411, 540, 1318, 1784
BstSLI	GKGCMC	1 cut(s)	2066
BstUI	CGCG	1 cut(s)	375
BstX2I	RGATCY	4 cut(s)	1208, 1285, 1880, 2009
BstYI	RGATCY	4 cut(s)	1208, 1285, 1880, 2009
Bsu36I	CCTNAGG	1 cut(s)	2097
BsuI	GTATCC	1 cut(s)	2103
BtgI	CCRYGG	2 cut(s)	883, 1129
BtgZI	GCGATG	1 cut(s)	390
BtsI	GCAGTG	1 cut(s)	1327
BtsIMutI	CAGTG	4 cut(s)	1198, 1282, 1327, 1374
BveI	ACCTGC	1 cut(s)	418
Cac8I	GCNNGC	2 cut(s)	240, 2186
CaiI	CAGNNNCTG	1 cut(s)	1469
CciI	TCATGA	2 cut(s)	631, 1174
CfoI	GCGC	2 cut(s)	375, 870
Cfr10I	RCCGGY	1 cut(s)	166
CseI	GACGC	2 cut(s)	170, 539
Csp6I	GTAC	5 cut(s)	7, 485, 712, 1466, 1941
CspCI	CAANNNNNGTGG	2 cut(s)	451, 486
CviQI	GTAC	5 cut(s)	7, 485, 712, 1466, 1941
DdeI	CTNAG	5 cut(s)	448, 975, 1027, 2097, 2229
DpnI	GATC	9 cut(s)	15, 636, 1077, 1210, 1272, 1287, 1335, 1882, 2011
DpnII	GATC	9 cut(s)	13, 634, 1075, 1208, 1270, 1285, 1333, 1880, 2009
DraI	TTTAAA	1 cut(s)	498
DraIII	CACNNNGTG	1 cut(s)	1279
EaeI	YGGCCR	3 cut(s)	880, 1006, 1399
Eam1104I	CTCTTC	5 cut(s)	202, 255, 858, 893, 1659
EarI	CTCTTC	5 cut(s)	202, 255, 858, 893, 1659
Eco130I	CCWWGG	2 cut(s)	883, 1726
Eco24I	GRGCYC	2 cut(s)	240, 2066
Eco47I	GGWCC	4 cut(s)	1475, 1490, 1640, 2081
Eco57I	CTGAAG	4 cut(s)	29, 608, 1491, 1854
Eco81I	CCTNAGG	1 cut(s)	2097
Eco88I	CYCGRG	1 cut(s)	2005
Eco91I	GGTNACC	3 cut(s)	1307, 1730, 1958
EcoNI	CCTNNNNNAGG	1 cut(s)	2199
EcoO109I	RGGNCCY	1 cut(s)	2062
EcoO65I	GGTNACC	3 cut(s)	1307, 1730, 1958
EcoRI	GAATTC	2 cut(s)	578, 1932
EcoT14I	CCWWGG	2 cut(s)	883, 1726
EcoT22I	ATGCAT	2 cut(s)	1570, 2145
EcoT38I	GRGCYC	2 cut(s)	240, 2066
ErhI	CCWWGG	2 cut(s)	883, 1726
FalI	AAGNNNNNCTT	4 cut(s)	782, 814, 939, 971
FaqI	GGGAC	2 cut(s)	857, 1626
FauI	CCCGC	3 cut(s)	66, 1641, 1743
FbaI	TGATCA	3 cut(s)	634, 1270, 1333
FriOI	GRGCYC	2 cut(s)	240, 2066
FspBI	CTAG	4 cut(s)	333, 465, 932, 2169
GlaI	GCGC	2 cut(s)	374, 869
GsuI	CTGGAG	2 cut(s)	198, 420
HapII	CCGG	4 cut(s)	167, 1398, 2116, 2147
HgaI	GACGC	2 cut(s)	170, 539
HhaI	GCGC	2 cut(s)	375, 870
Hin1I	GRCGYC	1 cut(s)	550
Hin6I	GCGC	2 cut(s)	373, 868
HinP1I	GCGC	2 cut(s)	373, 868
HincII	GTYRAC	3 cut(s)	907, 1509, 2123
HindII	GTYRAC	3 cut(s)	907, 1509, 2123
HindIII	AAGCTT	2 cut(s)	302, 781
HinfI	GANTC	9 cut(s)	358, 505, 535, 619, 661, 764, 1510, 1666, 1948
HpaII	CCGG	4 cut(s)	167, 1398, 2116, 2147
HphI	GGTGA	5 cut(s)	284, 701, 1430, 1724, 1952
Hpy166II	GTNNAC	6 cut(s)	487, 534, 907, 1509, 2081, 2123
Hpy188I	TCNGA	4 cut(s)	451, 820, 1204, 1953
Hpy188III	TCNNGA	7 cut(s)	215, 632, 773, 1175, 1382, 1562, 2007
Hpy8I	GTNNAC	6 cut(s)	487, 534, 907, 1509, 2081, 2123
HpyAV	CCTTC	9 cut(s)	540, 1066, 1105, 1135, 1322, 1466, 1514, 1905, 2119
HpyCH4III	ACNGT	6 cut(s)	278, 340, 394, 832, 855, 1277
HpyF3I	CTNAG	5 cut(s)	448, 975, 1027, 2097, 2229
Hsp92I	GRCGYC	1 cut(s)	550
HspAI	GCGC	2 cut(s)	373, 868
Ksp22I	TGATCA	3 cut(s)	634, 1270, 1333
Kzo9I	GATC	9 cut(s)	13, 634, 1075, 1208, 1270, 1285, 1333, 1880, 2009
LguI	GCTCTTC	1 cut(s)	858
LmnI	GCTCC	3 cut(s)	247, 1582, 1627
MaeI	CTAG	4 cut(s)	333, 465, 932, 2169
MaeIII	GTNAC	7 cut(s)	272, 773, 1049, 1147, 1307, 1730, 1958
MalI	GATC	9 cut(s)	15, 636, 1077, 1210, 1272, 1287, 1335, 1882, 2011
MboI	GATC	9 cut(s)	13, 634, 1075, 1208, 1270, 1285, 1333, 1880, 2009
MboII	GAAGA	8 cut(s)	189, 242, 614, 875, 910, 1190, 1646, 1860
MfeI	CAATTG	2 cut(s)	420, 673
MflI	RGATCY	4 cut(s)	1208, 1285, 1880, 2009
MhlI	GDGCHC	4 cut(s)	240, 1026, 1624, 2066
MlsI	TGGCCA	2 cut(s)	882, 1008
MluCI	AATT	9 cut(s)	143, 420, 578, 673, 1254, 1388, 1456, 1932, 2133
MluNI	TGGCCA	2 cut(s)	882, 1008
MlyI	GAGTC	3 cut(s)	529, 670, 1504
MmeI	TCCRAC	1 cut(s)	1519
Mox20I	TGGCCA	2 cut(s)	882, 1008
Mph1103I	ATGCAT	2 cut(s)	1570, 2145
MroXI	GAANNNNTTC	2 cut(s)	618, 768
MscI	TGGCCA	2 cut(s)	882, 1008
MseI	TTAA	9 cut(s)	497, 519, 606, 779, 834, 875, 960, 1032, 1392
MslI	CAYNNNNRTG	1 cut(s)	341
Msp20I	TGGCCA	2 cut(s)	882, 1008
MspA1I	CMGCKG	1 cut(s)	1783
MspCI	CTTAAG	1 cut(s)	778
MspI	CCGG	4 cut(s)	167, 1398, 2116, 2147
MunI	CAATTG	2 cut(s)	420, 673
MvnI	CGCG	1 cut(s)	375
NciI	CCSGG	3 cut(s)	1398, 2117, 2148
NcoI	CCATGG	1 cut(s)	883
NdeII	GATC	9 cut(s)	13, 634, 1075, 1208, 1270, 1285, 1333, 1880, 2009
NmuCI	GTSAC	5 cut(s)	272, 773, 1147, 1730, 1958
NsiI	ATGCAT	2 cut(s)	1570, 2145
NspI	RCATGY	2 cut(s)	2059, 2188
NspV	TTCGAA	2 cut(s)	361, 1070
OliI	CACNNNNGTG	1 cut(s)	341
PaeI	GCATGC	1 cut(s)	2188
PagI	TCATGA	2 cut(s)	631, 1174
PaqCI	CACCTGC	1 cut(s)	418
PciSI	GCTCTTC	1 cut(s)	858
PdmI	GAANNNNTTC	2 cut(s)	618, 768
PfeI	GAWTC	6 cut(s)	358, 505, 619, 764, 1666, 1948
PflMI	CCANNNNNTGG	2 cut(s)	1487, 1538
PfoI	TCCNGGA	2 cut(s)	283, 1486
PleI	GAGTC	3 cut(s)	529, 669, 1504
PpsI	GAGTC	3 cut(s)	529, 669, 1504
PshAI	GACNNNNGTC	1 cut(s)	1377
PshBI	ATTAAT	1 cut(s)	519
PsiI	TTATAA	1 cut(s)	2154
PspEI	GGTNACC	3 cut(s)	1307, 1730, 1958
PspOMI	GGGCCC	1 cut(s)	2062
PstI	CTGCAG	3 cut(s)	415, 1322, 1788
PstNI	CAGNNNCTG	1 cut(s)	1469
PsuI	RGATCY	4 cut(s)	1208, 1285, 1880, 2009
PvuII	CAGCTG	1 cut(s)	1783
RsaI	GTAC	5 cut(s)	8, 486, 713, 1467, 1942
RsaNI	GTAC	5 cut(s)	7, 485, 712, 1466, 1941
RseI	CAYNNNNRTG	1 cut(s)	341
SapI	GCTCTTC	1 cut(s)	858
SaqAI	TTAA	9 cut(s)	497, 519, 606, 779, 834, 875, 960, 1032, 1392
Sau3AI	GATC	9 cut(s)	13, 634, 1075, 1208, 1270, 1285, 1333, 1880, 2009
ScaI	AGTACT	1 cut(s)	713
SchI	GAGTC	3 cut(s)	529, 670, 1504
SduI	GDGCHC	4 cut(s)	240, 1026, 1624, 2066
SfcI	CTRYAG	4 cut(s)	411, 540, 1318, 1784
SfuI	TTCGAA	2 cut(s)	361, 1070
SinI	GGWCC	4 cut(s)	1475, 1490, 1640, 2081
SmiMI	CAYNNNNRTG	1 cut(s)	341
SmlI	CTYRAG	1 cut(s)	778
SmoI	CTYRAG	1 cut(s)	778
SpeI	ACTAGT	1 cut(s)	931
SphI	GCATGC	1 cut(s)	2188
Sse9I	AATT	9 cut(s)	143, 420, 578, 673, 1254, 1388, 1456, 1932, 2133
SspI	AATATT	1 cut(s)	1184
SspMI	CTAG	4 cut(s)	333, 465, 932, 2169
StyI	CCWWGG	2 cut(s)	883, 1726
TaaI	ACNGT	6 cut(s)	278, 340, 394, 832, 855, 1277
TaqI	TCGA	6 cut(s)	361, 515, 1070, 1138, 1513, 1831
TasI	AATT	9 cut(s)	143, 420, 578, 673, 1254, 1388, 1456, 1932, 2133
TatI	WGTACW	2 cut(s)	484, 711
TauI	GCSGC	3 cut(s)	160, 163, 1816
TfiI	GAWTC	6 cut(s)	358, 505, 619, 764, 1666, 1948
Tru1I	TTAA	9 cut(s)	497, 519, 606, 779, 834, 875, 960, 1032, 1392
Tru9I	TTAA	9 cut(s)	497, 519, 606, 779, 834, 875, 960, 1032, 1392
TscAI	CASTG	4 cut(s)	1198, 1282, 1327, 1381
TseFI	GTSAC	5 cut(s)	272, 773, 1147, 1730, 1958
Tsp45I	GTSAC	5 cut(s)	272, 773, 1147, 1730, 1958
TspGWI	ACGGA	2 cut(s)	1118, 1158
TspRI	CASTG	4 cut(s)	1198, 1282, 1327, 1381
Van91I	CCANNNNNTGG	2 cut(s)	1487, 1538
Vha464I	CTTAAG	1 cut(s)	778
VpaK11BI	GGWCC	4 cut(s)	1475, 1490, 1640, 2081
VspI	ATTAAT	1 cut(s)	519
XagI	CCTNNNNNAGG	1 cut(s)	2199
XapI	RAATTY	6 cut(s)	143, 578, 1254, 1388, 1932, 2133
XceI	RCATGY	2 cut(s)	2059, 2188
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1226
XmnI	GAANNNNTTC	2 cut(s)	618, 768
XspI	CTAG	4 cut(s)	333, 465, 932, 2169
ZrmI	AGTACT	1 cut(s)	713
Zsp2I	ATGCAT	2 cut(s)	1570, 2145

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.