Rh1CG058500

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000356
Physical Location & Seq
Forward (+)
11537908 .. 11547882
9975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG058500.1

Sequence Viewer

Length: 2019 bp
ATGGAAAGTGGTGGTAGAATTTTGTTGGATATTTGGTTAATTAGTAGCGAAAATGGTTTGTTTGTTGCAGTTCATGGTGATGGTTTTGGTGCAAAGAGGAACAGAAAGCAGACTCAGAGAAGGGCAGTTGATTACACTAGCACTGTCGTGCGGTATACACAGACTAGAATGTGGCAGCGGGATGCGAGGGATAGGACAGTATTGCAACCTACACCAGCAGCAGCAATTGACATGTTGCCGACAGTTGCCTATTCAAATAACCCATCTACGGGCTTTGCTGCAAAGTTTGTGCATACTTCCACAAACAAAGTCCGTAATTCAATTAATCGGGTTTTGTGGACTCCTCCTGGGAGGCGTCTTATTACAGGCTCTCAAAATGGGGAATTCACTCTTTGGAATGGCCAATCATTTAATTTTGAAATGATTCTTCAGGCTCATGATCAAGCAATCAGATCTATGGTCTGGAGTCATAATGAAAATTGGATGGTCTCTGGTGATGATGGAGGATCAATAAAGTATTGGCAGACCAACATGAATAATGTGAAGGCCAATAAATCTGCTCACAAAGAATCTGTTCAGGACTTAAGCTTCTGTAAGACTGACTTGAAGTTCTGTTCCTGTTCGAATGATACTTCTGTTAAAATATGGGATTTTGCACGGTGCCAAGAAGAGCGCACATTGACTGGCCATGGTTGGGATGTGAAGAGTGTTGACTGGCACCCTACAAAGTCTCTACTAGCTTCAGGTGGGAAAGACCAGCTTGTCAAACTTTGGGATGCTAAGTCGGGGCGAGAACTATGTTCATTTCATGGTCACAAAAATCTGGTGCAATCTGTTAAATGGAACCAAAATGGCAACTGGCTGCTAACTGCTTCTAAGGATCAAGTCATAAAGCTTTATGACATAAGGGCTATGAAGGAGCTTGAATCTTTCCGTGGGCATCGGAAGGAAGTGACTGCTCTGGCTTGGCATCCTTTTCATGAAGAATATTTTGTCAGTGGGAGTTCTGATGGATCCATTTTCCATTGGCTTGTTGGGCATGAGACTCCCCAGGTTGAAATTGTTAATGCACACGATAACAGTGTGTGGGATCTCCAATGGCACCCTATTGGCTATATTCTTTGCAGTGGTAGCAATGATCACACAACAAAGTTTTGGTGCAGAAACAGGCCAGGAGACACTGGTCGCGATAAATTTAACATCAATCAGAGTCAAGGTTACGGTGATCAAAACTCTGCTTTTGCTGGTCGCATGACGGGTAATTTTCCATTACACGATGGACCACCAACTCCAGGACCATTTACTCCCGGGGGAACTCGAAATGAAGGAACCATTCCAGGGGTTGGAGTAGCTATGCCGTTAGCCATACCATCCCTTGACGCATCTATTCAGGGAGAACAACAGCAACCTCATGCACATTCAATGCCTTTAGGAGCTCCTCCTCTCCCACCCGGTCCACATCCTTCTCTTCTTGCTGCAAATCAGCAGCAACCATATCATCAAAATGCGCAACAGCAACATCAGCAACACCAAGGTCACCCACAACAAATGCCCCCTTTGCCTATGCCACCTCCAAATATGCCACAGCTACAGCCTCCATCCCATTTACCCTTGCTGCCGCATCCTCATTTACGTCCACCACCCCAAATGCCCCCACTCGGTATGCCATCTTCAGTGTCCGGATCTTTGCCTATGCCTTCATCAGTTCCAACATCACTTTCAATGCCAATGGGCATGCAAGGTACAATGAATCAGATGGTTCATCAATTGCCACAAGGTCATTATATGGGCATGAACCCACCTCCACCTGCTGGAGGTTTCCCAAATGGGATGCCGAACATGCAGGGCCCATCTAATGCAAGTGGAGGGCAAATGTATTCTCATGCTGGCACCTTCAATCGTGCACAATCCGGACAAATGCCAATGCTACCGGGGTATAATCCCTACCAGCCCAGGGGTCAATCCGGTATGCCTCAGCCTCCACCAGGGCCACCACCACATGGGCAAACACCTCAATAG

Protein Analysis

672

Amino Acids

74.28

Weight (kDa)

8.92

Isoelectric Point (pI)

49.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_MABP1-WDR62_2nd PF24782 56 - 343 2.2e-14 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 84 - 268 1.1e-25 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 98 - 171 1e-05 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 98 - 176 2.4e-06 WDR3 second beta-propeller domain
WDR55 PF24796 100 - 177 6.6e-06 WDR55
Beta-prop_WDR3_1st PF25173 101 - 253 6.2e-23 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 104 - 277 5.5e-27 THOC3 beta-propeller domain
Beta-prop_IFT140_1st PF23383 107 - 193 2.3e-09 IFT140 first beta-propeller
WD40_Gbeta PF25391 108 - 311 3.8e-20 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 116 - 183 1.5e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 117 - 310 1.9e-31 Prp19 WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 138 - 254 1.3e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 141 - 174 9e-06 WD domain, G-beta repeat
EIF3I PF24805 143 - 223 1.7e-08 EIF3I
Beta-prop_TEP1_2nd PF25047 151 - 321 9.7e-15 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 164 - 269 9.9e-10 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 170 - 260 5.9e-10 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 170 - 285 1.9e-23 WDR5 beta-propeller domain
WDR55 PF24796 179 - 306 5.3e-14 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 183 - 344 3e-18 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 199 - 343 4.1e-19 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 203 - 291 4.9e-06 WDR90/POC16, second beta-propeller
Beta-prop_IFT140_1st PF23383 221 - 298 3.9e-06 IFT140 first beta-propeller
Beta-prop_WDR3_1st PF25173 221 - 343 1.6e-27 WDR3 first beta-propeller domain
WD40 PF00400 223 - 259 3.9e-09 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 225 - 306 1.2e-11 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 226 - 385 5.2e-14 EIF3I
Beta-prop_WDR3_2nd PF25172 226 - 346 5.4e-14 WDR3 second beta-propeller domain
Beta-prop_EML PF23409 227 - 333 3.5e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin PF25460 233 - 343 1.3e-06 Aladin seven-bladed propeller
Beta-prop_WDR19_1st PF23389 237 - 384 3.1e-08 WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 254 - 387 2e-10 WDR36/Utp21 first beta-propeller
WD40 PF00400 264 - 301 5.2e-08 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 265 - 388 2.2e-22 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 270 - 387 5.3e-21 WDR5 beta-propeller domain
ANAPC4_WD40 PF12894 271 - 324 7.8e-06 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_THOC3 PF25174 272 - 388 1.4e-22 THOC3 beta-propeller domain
Beta-prop_EIPR1 PF23609 272 - 340 1.9e-06 EIPR1 beta-propeller
WD40_WDHD1_1st PF24817 276 - 357 1.5e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 308 - 386 2.8e-10 Prp19 WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 309 - 385 8.4e-06 CAF1B/HIR1 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica Prupe.2G034300_v2.0.a1
pyrus_communis pycom02g24420 pycom07g02650
rosa_chinensis RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1816
AasI GACNNNNNNGTC 1 cut(s) 761
Acc16I TGCGCA 1 cut(s) 1509
Acc36I ACCTGC 1 cut(s) 1816
AccB1I GGYRCC 4 cut(s) 660, 717, 1101, 1889
AccB7I CCANNNNNTGG 3 cut(s) 1343, 1811, 2000
AccI GTMKAC 1 cut(s) 155
AccII CGCG 1 cut(s) 1188
AccIII TCCGGA 2 cut(s) 1679, 1910
AciI CCGC 3 cut(s) 151, 178, 1619
AclWI GGATC 6 cut(s) 514, 888, 1008, 1021, 1098, 1690
AcoI YGGCCR 2 cut(s) 400, 685
AcsI RAATTY 3 cut(s) 18, 383, 1193
AcuI CTGAAG 3 cut(s) 413, 726, 1656
AcyI GRCGYC 1 cut(s) 355
AfaI GTAC 1 cut(s) 1744
AflII CTTAAG 1 cut(s) 583
AflIII ACRYGT 1 cut(s) 231
AgsI TTSAA 9 cut(s) 255, 321, 419, 607, 926, 1058, 1422, 1722, 1897
AjnI CCWGG 7 cut(s) 346, 1050, 1171, 1291, 1336, 1952, 1984
AjuI GAANNNNNNNTTGG 2 cut(s) 1702, 1734
AleI CACNNNNGTG 1 cut(s) 146
AluBI AGCT 8 cut(s) 588, 740, 760, 895, 922, 1352, 1436, 1588
AluI AGCT 8 cut(s) 588, 740, 760, 895, 922, 1352, 1436, 1588
Alw21I GWGCWC 2 cut(s) 1438, 1906
Alw26I GTCTC 4 cut(s) 493, 735, 1037, 1170
Alw44I GTGCAC 1 cut(s) 1902
AlwI GGATC 6 cut(s) 514, 888, 1008, 1021, 1098, 1690
Ama87I CYCGRG 1 cut(s) 1307
Aor13HI TCCGGA 2 cut(s) 1679, 1910
AoxI GGCC 6 cut(s) 400, 546, 685, 1169, 1846, 1988
ApaI GGGCCC 1 cut(s) 1850
ApaLI GTGCAC 1 cut(s) 1902
ApeKI GCWGC 8 cut(s) 175, 218, 221, 278, 862, 1475, 1486, 1615
ApoI RAATTY 3 cut(s) 18, 383, 1193
AseI ATTAAT 1 cut(s) 324
Asp700I GAANNNNTTC 2 cut(s) 423, 573
AspLEI GCGC 2 cut(s) 675, 1510
AspS9I GGNCC 6 cut(s) 1280, 1295, 1454, 1846, 1847, 1988
AsuC2I CCSGG 4 cut(s) 1308, 1309, 1452, 1932
AsuHPI GGTGA 4 cut(s) 89, 506, 1235, 1529
AsuII TTCGAA 1 cut(s) 623
AvaI CYCGRG 1 cut(s) 1307
AvaII GGWCC 3 cut(s) 1280, 1295, 1454
BaeGI GKGCMC 2 cut(s) 1850, 1906
BaeI ACNNNNGTAYC 2 cut(s) 621, 654
BalI TGGCCA 2 cut(s) 402, 687
BamHI GGATCC 1 cut(s) 1013
BanI GGYRCC 4 cut(s) 660, 717, 1101, 1889
BanII GRGCYC 2 cut(s) 1438, 1850
BarI GAAGNNNNNNTAC 2 cut(s) 1654, 1686
Bbv12I GWGCWC 2 cut(s) 1438, 1906
BbvCI CCTCAGC 1 cut(s) 1974
BbvI GCAGC 8 cut(s) 187, 230, 233, 265, 849, 1462, 1498, 1602
BceAI ACGGC 1 cut(s) 1342
BciT130I CCWGG 7 cut(s) 348, 1052, 1173, 1293, 1338, 1954, 1986
BclI TGATCA 3 cut(s) 439, 1138, 1225
BcnI CCSGG 4 cut(s) 1308, 1309, 1452, 1932
BcoDI GTCTC 4 cut(s) 493, 735, 1037, 1170
BfaI CTAG 3 cut(s) 138, 165, 737
BfmI CTRYAG 1 cut(s) 1589
BfrI CTTAAG 1 cut(s) 583
BfuAI ACCTGC 1 cut(s) 1816
BglII AGATCT 1 cut(s) 452
BisI GCNGC 9 cut(s) 176, 219, 222, 279, 863, 1476, 1487, 1616, 1619
BlsI GCNGC 9 cut(s) 177, 220, 223, 280, 864, 1477, 1488, 1617, 1620
Bme18I GGWCC 3 cut(s) 1280, 1295, 1454
BmeT110I CYCGRG 1 cut(s) 1307
BmgT120I GGNCC 6 cut(s) 1280, 1295, 1454, 1846, 1847, 1988
BmiI GGNNCC 8 cut(s) 662, 719, 845, 1015, 1103, 1330, 1848, 1891
BmsI GCATC 7 cut(s) 172, 766, 949, 979, 1391, 1630, 1821
BoxI GACNNNNGTC 1 cut(s) 1182
BpmI CTGGAG 3 cut(s) 484, 1275, 1833
Bpu10I CCTNAGC 1 cut(s) 1974
Bpu14I TTCGAA 1 cut(s) 623
BpuMI CCSGG 4 cut(s) 1308, 1309, 1452, 1932
BsaHI GRCGYC 1 cut(s) 355
BsaI GGTCTC 1 cut(s) 493
BsaWI WCCGGW 3 cut(s) 1679, 1910, 1964
Bse1I ACTGG 4 cut(s) 688, 719, 863, 1186
Bse3DI GCAATG 1 cut(s) 1141
BseAI TCCGGA 2 cut(s) 1679, 1910
BseBI CCWGG 7 cut(s) 348, 1052, 1173, 1293, 1338, 1954, 1986
BseMI GCAATG 1 cut(s) 1141
BseMII CTCAG 2 cut(s) 128, 1988
BseNI ACTGG 4 cut(s) 688, 719, 863, 1186
BseRI GAGGAG 3 cut(s) 333, 1428, 1431
BseSI GKGCMC 2 cut(s) 1850, 1906
BseXI GCAGC 8 cut(s) 187, 230, 233, 265, 849, 1462, 1498, 1602
BsgI GTGCAG 1 cut(s) 1180
Bsh1236I CGCG 1 cut(s) 1188
BshFI GGCC 6 cut(s) 402, 548, 687, 1171, 1848, 1990
BshNI GGYRCC 4 cut(s) 660, 717, 1101, 1889
BsiHKAI GWGCWC 2 cut(s) 1438, 1906
BsiHKCI CYCGRG 1 cut(s) 1307
BsiSI CCGG 6 cut(s) 1308, 1452, 1680, 1911, 1931, 1965
BsmAI GTCTC 4 cut(s) 493, 735, 1037, 1170
BsnI GGCC 6 cut(s) 402, 548, 687, 1171, 1848, 1990
Bso31I GGTCTC 1 cut(s) 493
BsoBI CYCGRG 1 cut(s) 1307
Bsp119I TTCGAA 1 cut(s) 623
Bsp120I GGGCCC 1 cut(s) 1846
Bsp1286I GDGCHC 3 cut(s) 1438, 1850, 1906
Bsp13I TCCGGA 2 cut(s) 1679, 1910
Bsp143I GATC 9 cut(s) 439, 452, 506, 880, 1013, 1090, 1138, 1225, 1682
Bsp19I CCATGG 1 cut(s) 688
Bsp68I TCGCGA 1 cut(s) 1188
BspACI CCGC 3 cut(s) 151, 178, 1619
BspANI GGCC 6 cut(s) 402, 548, 687, 1171, 1848, 1990
BspCNI CTCAG 2 cut(s) 127, 1987
BspEI TCCGGA 2 cut(s) 1679, 1910
BspFNI CGCG 1 cut(s) 1188
BspHI TCATGA 2 cut(s) 436, 979
BspLI GGNNCC 8 cut(s) 662, 719, 845, 1015, 1103, 1330, 1848, 1891
BspMI ACCTGC 1 cut(s) 1816
BspPI GGATC 6 cut(s) 514, 888, 1008, 1021, 1098, 1690
BspQI GCTCTTC 1 cut(s) 663
BspT104I TTCGAA 1 cut(s) 623
BspT107I GGYRCC 4 cut(s) 660, 717, 1101, 1889
BspTI CTTAAG 1 cut(s) 583
BspTNI GGTCTC 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 1141
BsrI ACTGG 4 cut(s) 688, 719, 863, 1186
BssMI GATC 9 cut(s) 439, 452, 506, 880, 1013, 1090, 1138, 1225, 1682
BssNAI GTATAC 1 cut(s) 156
BssNI GRCGYC 1 cut(s) 355
BssT1I CCWWGG 2 cut(s) 688, 1531
Bst1107I GTATAC 1 cut(s) 156
Bst2UI CCWGG 7 cut(s) 348, 1052, 1173, 1293, 1338, 1954, 1986
Bst4CI ACNGT 6 cut(s) 145, 199, 244, 660, 1082, 1223
Bst6I CTCTTC 3 cut(s) 663, 698, 1473
BstACI GRCGYC 1 cut(s) 355
BstAFI CTTAAG 1 cut(s) 583
BstBI TTCGAA 1 cut(s) 623
BstC8I GCNNGC 2 cut(s) 1736, 1888
BstDEI CTNAG 4 cut(s) 114, 780, 876, 1974
BstDSI CCRYGG 2 cut(s) 688, 934
BstEII GGTNACC 1 cut(s) 1535
BstENI CCTNNNNNAGG 2 cut(s) 1812, 1983
BstFNI CGCG 1 cut(s) 1188
BstHHI GCGC 2 cut(s) 675, 1510
BstKTI GATC 9 cut(s) 442, 455, 509, 883, 1016, 1093, 1141, 1228, 1685
BstMAI GTCTC 4 cut(s) 493, 735, 1037, 1170
BstMBI GATC 9 cut(s) 439, 452, 506, 880, 1013, 1090, 1138, 1225, 1682
BstMWI GCNNNNNNNGC 5 cut(s) 1036, 1131, 1522, 1558, 1840
BstNI CCWGG 7 cut(s) 348, 1052, 1173, 1293, 1338, 1954, 1986
BstNSI RCATGY 3 cut(s) 235, 1738, 1843
BstPAI GACNNNNGTC 1 cut(s) 1182
BstPI GGTNACC 1 cut(s) 1535
BstSFI CTRYAG 1 cut(s) 1589
BstSLI GKGCMC 2 cut(s) 1850, 1906
BstUI CGCG 1 cut(s) 1188
BstV1I GCAGC 8 cut(s) 187, 230, 233, 265, 849, 1462, 1498, 1602
BstX2I RGATCY 4 cut(s) 452, 1013, 1090, 1682
BstYI RGATCY 4 cut(s) 452, 1013, 1090, 1682
BstZ17I GTATAC 1 cut(s) 156
BsuRI GGCC 6 cut(s) 402, 548, 687, 1171, 1848, 1990
BtgI CCRYGG 2 cut(s) 688, 934
BtsI GCAGTG 1 cut(s) 1132
BtsIMutI CAGTG 6 cut(s) 141, 1003, 1087, 1132, 1179, 1680
BtuMI TCGCGA 1 cut(s) 1188
BveI ACCTGC 1 cut(s) 1816
Cac8I GCNNGC 2 cut(s) 1736, 1888
CciI TCATGA 2 cut(s) 436, 979
CfoI GCGC 2 cut(s) 675, 1510
Cfr13I GGNCC 6 cut(s) 1280, 1295, 1454, 1846, 1847, 1988
Cfr9I CCCGGG 1 cut(s) 1307
CseI GACGC 2 cut(s) 344, 1388
Csp6I GTAC 1 cut(s) 1743
CviQI GTAC 1 cut(s) 1743
DdeI CTNAG 4 cut(s) 114, 780, 876, 1974
DpnI GATC 9 cut(s) 441, 454, 508, 882, 1015, 1092, 1140, 1227, 1684
DpnII GATC 9 cut(s) 439, 452, 506, 880, 1013, 1090, 1138, 1225, 1682
DrdI GACNNNNNNGTC 1 cut(s) 761
DseDI GACNNNNNNGTC 1 cut(s) 761
EaeI YGGCCR 2 cut(s) 400, 685
Eam1104I CTCTTC 3 cut(s) 663, 698, 1473
EarI CTCTTC 3 cut(s) 663, 698, 1473
Ecl136II GAGCTC 1 cut(s) 1436
Eco130I CCWWGG 2 cut(s) 688, 1531
Eco24I GRGCYC 2 cut(s) 1438, 1850
Eco31I GGTCTC 1 cut(s) 493
Eco47I GGWCC 3 cut(s) 1280, 1295, 1454
Eco53kI GAGCTC 1 cut(s) 1436
Eco57I CTGAAG 3 cut(s) 413, 726, 1656
Eco88I CYCGRG 1 cut(s) 1307
Eco91I GGTNACC 1 cut(s) 1535
EcoICRI GAGCTC 1 cut(s) 1436
EcoNI CCTNNNNNAGG 2 cut(s) 1812, 1983
EcoO109I RGGNCCY 1 cut(s) 1846
EcoO65I GGTNACC 1 cut(s) 1535
EcoRI GAATTC 1 cut(s) 383
EcoRII CCWGG 7 cut(s) 346, 1050, 1171, 1291, 1336, 1952, 1984
EcoT14I CCWWGG 2 cut(s) 688, 1531
EcoT38I GRGCYC 2 cut(s) 1438, 1850
ErhI CCWWGG 2 cut(s) 688, 1531
FalI AAGNNNNNCTT 4 cut(s) 587, 619, 744, 776
FauI CCCGC 1 cut(s) 171
FbaI TGATCA 3 cut(s) 439, 1138, 1225
FblI GTMKAC 1 cut(s) 155
Fnu4HI GCNGC 9 cut(s) 176, 219, 222, 279, 863, 1476, 1487, 1616, 1619
FriOI GRGCYC 2 cut(s) 1438, 1850
Fsp4HI GCNGC 9 cut(s) 176, 219, 222, 279, 863, 1476, 1487, 1616, 1619
FspBI CTAG 3 cut(s) 138, 165, 737
FspI TGCGCA 1 cut(s) 1509
GlaI GCGC 2 cut(s) 674, 1509
GluI GCNGC 9 cut(s) 176, 219, 222, 279, 863, 1476, 1487, 1616, 1619
GsuI CTGGAG 3 cut(s) 484, 1275, 1833
HaeIII GGCC 6 cut(s) 402, 548, 687, 1171, 1848, 1990
HapII CCGG 6 cut(s) 1308, 1452, 1680, 1911, 1931, 1965
HgaI GACGC 2 cut(s) 344, 1388
HhaI GCGC 2 cut(s) 675, 1510
Hin1I GRCGYC 1 cut(s) 355
Hin6I GCGC 2 cut(s) 673, 1508
HinP1I GCGC 2 cut(s) 673, 1508
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HindIII AAGCTT 2 cut(s) 586, 893
HinfI GANTC 9 cut(s) 112, 340, 424, 466, 569, 926, 1045, 1210, 1750
HpaII CCGG 6 cut(s) 1308, 1452, 1680, 1911, 1931, 1965
HphI GGTGA 4 cut(s) 89, 506, 1235, 1529
Hpy166II GTNNAC 6 cut(s) 156, 339, 712, 1457, 1637, 1904
Hpy188I TCNGA 6 cut(s) 117, 452, 945, 1009, 1209, 1755
Hpy188III TCNNGA 7 cut(s) 437, 463, 578, 980, 1187, 1680, 1911
Hpy8I GTNNAC 6 cut(s) 156, 339, 712, 1457, 1637, 1904
HpyAV CCTTC 8 cut(s) 114, 538, 910, 940, 1319, 1473, 1707, 1903
HpyCH4III ACNGT 6 cut(s) 145, 199, 244, 660, 1082, 1223
HpyCH4IV ACGT 1 cut(s) 1633
HpyF10VI GCNNNNNNNGC 5 cut(s) 1036, 1131, 1522, 1558, 1840
HpyF3I CTNAG 4 cut(s) 114, 780, 876, 1974
HpySE526I ACGT 1 cut(s) 1633
Hsp92I GRCGYC 1 cut(s) 355
HspAI GCGC 2 cut(s) 673, 1508
Kpn2I TCCGGA 2 cut(s) 1679, 1910
Ksp22I TGATCA 3 cut(s) 439, 1138, 1225
Kzo9I GATC 9 cut(s) 439, 452, 506, 880, 1013, 1090, 1138, 1225, 1682
LguI GCTCTTC 1 cut(s) 663
LmnI GCTCC 3 cut(s) 919, 1433, 1441
Lsp1109I GCAGC 8 cut(s) 187, 230, 233, 265, 849, 1462, 1498, 1602
LweI GCATC 7 cut(s) 172, 766, 949, 979, 1391, 1630, 1821
MaeI CTAG 3 cut(s) 138, 165, 737
MaeII ACGT 1 cut(s) 1633
MaeIII GTNAC 4 cut(s) 812, 952, 1217, 1535
MalI GATC 9 cut(s) 441, 454, 508, 882, 1015, 1092, 1140, 1227, 1684
MboI GATC 9 cut(s) 439, 452, 506, 880, 1013, 1090, 1138, 1225, 1682
MboII GAAGA 6 cut(s) 419, 680, 715, 995, 1460, 1662
MfeI CAATTG 2 cut(s) 225, 1766
MflI RGATCY 4 cut(s) 452, 1013, 1090, 1682
MhlI GDGCHC 3 cut(s) 1438, 1850, 1906
MlsI TGGCCA 2 cut(s) 402, 687
MluNI TGGCCA 2 cut(s) 402, 687
MlyI GAGTC 5 cut(s) 106, 334, 475, 1039, 1219
MmeI TCCRAC 3 cut(s) 6, 1324, 1733
Mox20I TGGCCA 2 cut(s) 402, 687
MroI TCCGGA 2 cut(s) 1679, 1910
MroXI GAANNNNTTC 2 cut(s) 423, 573
MscI TGGCCA 2 cut(s) 402, 687
MseI TTAA 8 cut(s) 38, 324, 411, 584, 639, 837, 1065, 1197
MslI CAYNNNNRTG 3 cut(s) 78, 146, 1503
Msp20I TGGCCA 2 cut(s) 402, 687
MspA1I CMGCKG 1 cut(s) 178
MspCI CTTAAG 1 cut(s) 583
MspI CCGG 6 cut(s) 1308, 1452, 1680, 1911, 1931, 1965
MunI CAATTG 2 cut(s) 225, 1766
MvaI CCWGG 7 cut(s) 348, 1052, 1173, 1293, 1338, 1954, 1986
MvnI CGCG 1 cut(s) 1188
MwoI GCNNNNNNNGC 5 cut(s) 1036, 1131, 1522, 1558, 1840
NciI CCSGG 4 cut(s) 1308, 1309, 1452, 1932
NcoI CCATGG 1 cut(s) 688
NdeII GATC 9 cut(s) 439, 452, 506, 880, 1013, 1090, 1138, 1225, 1682
NlaIV GGNNCC 8 cut(s) 662, 719, 845, 1015, 1103, 1330, 1848, 1891
NmuCI GTSAC 3 cut(s) 812, 952, 1535
NruI TCGCGA 1 cut(s) 1188
NsbI TGCGCA 1 cut(s) 1509
NspI RCATGY 3 cut(s) 235, 1738, 1843
NspV TTCGAA 1 cut(s) 623
OliI CACNNNNGTG 1 cut(s) 146
PaeI GCATGC 1 cut(s) 1738
PagI TCATGA 2 cut(s) 436, 979
PaqCI CACCTGC 1 cut(s) 1816
PasI CCCWGGG 1 cut(s) 1953
PciI ACATGT 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 663
PdmI GAANNNNTTC 2 cut(s) 423, 573
PfeI GAWTC 4 cut(s) 424, 569, 926, 1750
PflMI CCANNNNNTGG 3 cut(s) 1343, 1811, 2000
PfoI TCCNGGA 1 cut(s) 1291
PkrI GCNGC 9 cut(s) 177, 220, 223, 280, 864, 1477, 1488, 1617, 1620
PleI GAGTC 5 cut(s) 106, 334, 474, 1039, 1218
PpsI GAGTC 5 cut(s) 106, 334, 474, 1039, 1218
PscI ACATGT 1 cut(s) 231
PshAI GACNNNNGTC 1 cut(s) 1182
PshBI ATTAAT 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 1438
Psp6I CCWGG 7 cut(s) 346, 1050, 1171, 1291, 1336, 1952, 1984
PspEI GGTNACC 1 cut(s) 1535
PspGI CCWGG 7 cut(s) 346, 1050, 1171, 1291, 1336, 1952, 1984
PspN4I GGNNCC 8 cut(s) 662, 719, 845, 1015, 1103, 1330, 1848, 1891
PspOMI GGGCCC 1 cut(s) 1846
PspPI GGNCC 6 cut(s) 1280, 1295, 1454, 1846, 1847, 1988
PsuI RGATCY 4 cut(s) 452, 1013, 1090, 1682
RruI TCGCGA 1 cut(s) 1188
RsaI GTAC 1 cut(s) 1744
RsaNI GTAC 1 cut(s) 1743
RseI CAYNNNNRTG 3 cut(s) 78, 146, 1503
SacI GAGCTC 1 cut(s) 1438
SapI GCTCTTC 1 cut(s) 663
SaqAI TTAA 8 cut(s) 38, 324, 411, 584, 639, 837, 1065, 1197
SatI GCNGC 9 cut(s) 176, 219, 222, 279, 863, 1476, 1487, 1616, 1619
Sau3AI GATC 9 cut(s) 439, 452, 506, 880, 1013, 1090, 1138, 1225, 1682
Sau96I GGNCC 6 cut(s) 1280, 1295, 1454, 1846, 1847, 1988
SchI GAGTC 5 cut(s) 106, 334, 475, 1039, 1219
SduI GDGCHC 3 cut(s) 1438, 1850, 1906
SfaNI GCATC 7 cut(s) 172, 766, 949, 979, 1391, 1630, 1821
SfcI CTRYAG 1 cut(s) 1589
SfuI TTCGAA 1 cut(s) 623
SinI GGWCC 3 cut(s) 1280, 1295, 1454
SmaI CCCGGG 1 cut(s) 1309
SmiMI CAYNNNNRTG 3 cut(s) 78, 146, 1503
SmlI CTYRAG 1 cut(s) 583
SmoI CTYRAG 1 cut(s) 583
SphI GCATGC 1 cut(s) 1738
SsiI CCGC 3 cut(s) 151, 178, 1619
SspI AATATT 1 cut(s) 989
SspMI CTAG 3 cut(s) 138, 165, 737
SstI GAGCTC 1 cut(s) 1438
StyI CCWWGG 2 cut(s) 688, 1531
TaaI ACNGT 6 cut(s) 145, 199, 244, 660, 1082, 1223
TaiI ACGT 1 cut(s) 1636
TaqI TCGA 2 cut(s) 623, 1318
TauI GCSGC 1 cut(s) 1621
TfiI GAWTC 4 cut(s) 424, 569, 926, 1750
Tru1I TTAA 8 cut(s) 38, 324, 411, 584, 639, 837, 1065, 1197
Tru9I TTAA 8 cut(s) 38, 324, 411, 584, 639, 837, 1065, 1197
TscAI CASTG 6 cut(s) 148, 1003, 1087, 1132, 1186, 1680
TseFI GTSAC 3 cut(s) 812, 952, 1535
TseI GCWGC 8 cut(s) 175, 218, 221, 278, 862, 1475, 1486, 1615
Tsp45I GTSAC 3 cut(s) 812, 952, 1535
TspGWI ACGGA 2 cut(s) 302, 923
TspMI CCCGGG 1 cut(s) 1307
TspRI CASTG 6 cut(s) 148, 1003, 1087, 1132, 1186, 1680
Van91I CCANNNNNTGG 3 cut(s) 1343, 1811, 2000
Vha464I CTTAAG 1 cut(s) 583
VneI GTGCAC 1 cut(s) 1902
VpaK11BI GGWCC 3 cut(s) 1280, 1295, 1454
VspI ATTAAT 1 cut(s) 324
XagI CCTNNNNNAGG 2 cut(s) 1812, 1983
XapI RAATTY 3 cut(s) 18, 383, 1193
XceI RCATGY 3 cut(s) 235, 1738, 1843
XcmI CCANNNNNNNNNTGG 1 cut(s) 1031
XmaI CCCGGG 1 cut(s) 1307
XmiI GTMKAC 1 cut(s) 155
XmnI GAANNNNTTC 2 cut(s) 423, 573
XspI CTAG 3 cut(s) 138, 165, 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.