Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0000356

Physical Location & Seq

Genomic Coordinates Reverse (-)

34507607 .. 34519105

Gene Structure 11499 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1288700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGATGTACCCTGATCCACAACAGCAACACCACCAACAGCAGCACCCGCAACATCAGCAGCAGCATTTGCAACAACATCAGCAGCAGCAGCAGCATTTGCAACAACAGCAGCAGCAGCATTTGCAACAACACCAGCAACAGCATTTGCAACTACACCAGCAACATCAGCAGCAAGGTGGAGAATTTCATAGGGGCCCGCCACCACCGGCCCCAATGATGCGTCAGCCGTCGGCATCTTCAACTAATATGCCCCCAGAGTATCACAACCAACCTGGGCTCGCTCCTCCTTATGATGCTCATGCTGACAGTTTTCCTGGAAAAAGGATGAAAAAGCTTACACACAGGAGGGCAGTTGATTATACTAGCACTGTCGTGCGATATATGCAGATTCGAATGTGGCAGCGCGATGCAAGGGATAGAACAGTATTGCAACCCACACCTGCAGCAGCAATTGATATGTTGCCGTCAGTTGCCTACTCAGATAATCCATCCACTAGCTTTGCTGCAAAGTTTGTACACACTTCTTTAAACAAGAATCGTTGTCCGATTAATCGGGTTTTGTGGACTCCTACAGGAAGACGTCTTATTACTGGCTCTCAAAGTGGGGAATTCACTCTTTGGAATGGACAATCATTTAACTTTGAAATGATTCTTCAGGCTCATGATCAAGCAATCAGGTCTATGGTATGGAGTCATAATGACAATTGGATGGTCTCTGGTGATGATGGCGGTGCAATAAAGTATTGGCAGAACAACATGAATAATGTAAAGGCAAATAAATCTGCTCACAAAGAATCTGTTCGCGACTTAAGTTTCTGCAAGACTGACTTGAAATTCTGTTCCTGCTCCGATGATAATACTGTTAAAGTTTGGGACTTTGCACGGTGCCAAGAAGAGCGCACATTGACTGGTAAAGCAGGCCATGGTTGGGATGTAAAGAGTGTTGACTGGCACCCTACTAAATCTCTACTAGTTTCAGGTGGGAAAGACAGTCTTGTTAAACTTTGGGATGCTAAGTCAGGGCGAGAACTCTGTTCGTTTCATGGCCATAAAAATATGGTGCTCTCGGTTAAATGGAACCAAAATGGTAACTGGGTGCTAACTGCTTCGAAGGATCAAATCATCAAGGTTTACGACATAAGGGCCATGAAGGAACTTGCATCCTTCCGCGGGCATCGAAAGGATGTGACTGCTCTGGCTTGGCATCCGTTTCATGAAGAATATTTTGTTAGTGGGAGTTTTGATGGATCCATTTACCATTGGCTTGTTGGGCATGAAACTCCCCAGGTTGAAATTCCCAATGCACATGATCACAGTGTGTGGGATCTTGCATGGCATCCTATTGGTTATCTTCTCTGCAGTGGTAGCAATGATCACACTACGAAGTTTTGGTGCAGAAATAGGCCAGGAGATACTGGTCATGATAAATCTAACACCGGCCAAAATCAAGGTTATGGTGAGCAAAACCCTGCTTTTGGTGGTCGCATGACTGGTAATTTTCCAGTACCTGAAGGACCACCAACTCCTGGACCATTTGTTCCTGGGTTGACTCGAAATGAAGGAACTATTCCAGGTGTTGGAGTTGCTATGCCGTTATCTCTTGATGCATCAAATCAGGGGGAGCAACAGCAGCCTCATGCACTATCGATGGGGTTAGGTGCTCCTCCTCTCCCGCCTGGTCCACACCCCTCTCTTCTTGCTGCGACTCAGCAGCAGCAATATCAACAAAATCCTCAACAGATGCCACAACAGCAACAGCAACACCAACACCAAGGTCACCCGCAACAAATGCCCCCTATGCCTATGGCACCTCCAAATATGCCACATCTGCAGCCTCCATCCCATTTACCCTTGCTGCCGCATCCTCATTTACATCGACCGCCACCCCAAATGCCCCCACTTGGTATGCCATCTTCAGGGCCTGGATCTATGCCTTCATCTGTTCCTACCTCACATTCAATGCCAATGGGAATTCAAGGTACAATGAATCAGATGGGTCACCCATTGCCACAAGGTCATTTTATGGGCATGAACCCAATGCACTCGGGATCTCTACCTACAAGCGGTGGAGGTTTTCCAAATGGTATGCCAAACATGCAGGGCCAAACAAATGCAAGTGGAGCTCAAATGTATCCTCAGGGTGGTGCCTTCAATCGGAGTCAGCCTGGGCAAATTCCCATGCATCCGGGGTATAATCCTTATCAGTCTAGGGGTCAGTCTGGTATGCCCCAACCTCCACCAGGGCCACCACCGCACGGGCAAACACCTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

WD40

Protein Analysis

758

Amino Acids

84.44

Weight (kDa)

8.85

Isoelectric Point (pI)

55.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_MABP1-WDR62_2nd	PF24782	129 - 421	1.7e-14	MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz	PF24807	174 - 346	1.2e-22	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	175 - 312	1.8e-17	WDR3 first beta-propeller domain
Beta-prop_IFT140_1st	PF23383	177 - 264	4.7e-10	IFT140 first beta-propeller
Beta-prop_WDR5	PF25175	177 - 254	3e-07	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	179 - 293	9.6e-06	WDR36/Utp21 first beta-propeller
Beta-prop_THOC3	PF25174	183 - 253	9.9e-06	THOC3 beta-propeller domain
WD40_Gbeta	PF25391	183 - 389	3.8e-17	G protein beta WD-40 repeat protein
Beta-prop_EML	PF23409	189 - 258	2.7e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19	PF24814	192 - 388	5e-29	Prp19 WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	216 - 304	7.1e-06	CAF1B/HIR1 beta-propeller domain
EIF3I	PF24805	217 - 300	2.4e-08	EIF3I
Beta-prop_WDR5	PF25175	245 - 360	5.2e-21	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	252 - 347	1.1e-07	WDHD1 first WD40 domain
WDR55	PF24796	254 - 422	3.8e-16	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	258 - 421	2.3e-16	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	261 - 292	4.9e-06	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	263 - 385	5.9e-24	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	274 - 418	7.1e-18	Echinoderm microtubule-associated protein second beta-propeller
WD40	PF00400	306 - 337	2.7e-07	WD domain, G-beta repeat
EIF3I	PF24805	306 - 463	2.8e-11	EIF3I
Beta-prop_WDR3_1st	PF25173	306 - 383	2.5e-17	WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	307 - 385	1.2e-09	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	307 - 424	6.5e-13	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	307 - 411	3.1e-08	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin	PF25460	311 - 420	1.6e-06	Aladin seven-bladed propeller
Beta-prop_TEP1_2nd	PF25047	312 - 397	1.9e-09	TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	327 - 465	9.3e-10	WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz	PF24807	334 - 466	6.2e-22	CDC20/Fizzy WD40 domain
WD40	PF00400	342 - 379	2.9e-08	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	348 - 465	1.3e-19	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	350 - 465	5.2e-21	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	354 - 451	1.3e-10	WDHD1 first WD40 domain
WD40_Prp19	PF24814	355 - 464	1.7e-16	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	377 - 464	3.1e-10	WDR3 first beta-propeller domain
WD40	PF00400	384 - 421	2e-06	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	388 - 463	4.9e-06	CAF1B/HIR1 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000356)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca	FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica	MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica	Prupe.2G034300_v2.0.a1
pyrus_communis	pycom02g24420 pycom07g02650
rosa_chinensis	RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata	RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora	Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii	Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa	Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha	Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana	Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	448
AatII	GACGTC	1 cut(s)	583
Acc36I	ACCTGC	1 cut(s)	448
AccB1I	GGYRCC	4 cut(s)	885, 951, 1807, 2144
AccB7I	CCANNNNNTGG	2 cut(s)	1526, 1577
AccII	CGCG	3 cut(s)	405, 804, 1170
AclWI	GGATC	7 cut(s)	8, 1122, 1242, 1255, 1332, 1933, 2056
AcoI	YGGCCR	2 cut(s)	1045, 1438
AcsI	RAATTY	6 cut(s)	182, 608, 833, 1293, 1971, 2172
AcuI	CTGAAG	3 cut(s)	638, 1530, 1899
AcyI	GRCGYC	1 cut(s)	580
AdeI	CACNNNGTG	1 cut(s)	1318
AfaI	GTAC	4 cut(s)	8, 516, 1506, 1981
AflII	CTTAAG	1 cut(s)	808
AgsI	TTSAA	7 cut(s)	240, 644, 832, 1292, 1959, 1976, 2152
AhlI	ACTAGT	1 cut(s)	970
AleI	CACNNNNGTG	1 cut(s)	371
AloI	GAACNNNNNNTCC	2 cut(s)	1521, 1553
AluBI	AGCT	3 cut(s)	334, 498, 2123
AluI	AGCT	3 cut(s)	334, 498, 2123
Alw21I	GWGCWC	3 cut(s)	1065, 1663, 2125
Alw26I	GTCTC	1 cut(s)	718
AlwI	GGATC	7 cut(s)	8, 1122, 1242, 1255, 1332, 1933, 2056
AlwNI	CAGNNNCTG	2 cut(s)	1508, 1922
Ama87I	CYCGRG	1 cut(s)	2044
ApaI	GGGCCC	1 cut(s)	197
ApoI	RAATTY	6 cut(s)	182, 608, 833, 1293, 1971, 2172
AseI	ATTAAT	1 cut(s)	549
Asp700I	GAANNNNTTC	2 cut(s)	648, 798
AspLEI	GCGC	2 cut(s)	405, 900
AsuC2I	CCSGG	1 cut(s)	2187
AsuHPI	GGTGA	4 cut(s)	731, 1469, 1769, 1991
AsuII	TTCGAA	2 cut(s)	391, 1109
AvaI	CYCGRG	1 cut(s)	2044
AvaII	GGWCC	3 cut(s)	1514, 1529, 1679
AxyI	CCTNAGG	1 cut(s)	2136
BaeGI	GKGCMC	1 cut(s)	197
BalI	TGGCCA	1 cut(s)	1047
BamHI	GGATCC	1 cut(s)	1247
BanI	GGYRCC	4 cut(s)	885, 951, 1807, 2144
BanII	GRGCYC	3 cut(s)	197, 279, 2125
BarI	GAAGNNNNNNTAC	2 cut(s)	1897, 1929
BbsI	GAAGAC	1 cut(s)	583
Bbv12I	GWGCWC	3 cut(s)	1065, 1663, 2125
BccI	CCATC	8 cut(s)	496, 703, 719, 1238, 1642, 1846, 1918, 1987
BceAI	ACGGC	3 cut(s)	211, 448, 1576
BciVI	GTATCC	1 cut(s)	2142
BclI	TGATCA	3 cut(s)	664, 1309, 1372
BcnI	CCSGG	1 cut(s)	2187
BcoDI	GTCTC	1 cut(s)	718
BcuI	ACTAGT	1 cut(s)	970
BfaI	CTAG	4 cut(s)	363, 495, 971, 2208
BfmI	CTRYAG	4 cut(s)	441, 570, 1357, 1829
BfrI	CTTAAG	1 cut(s)	808
BfuAI	ACCTGC	1 cut(s)	448
BfuI	GTATCC	1 cut(s)	2142
Bme18I	GGWCC	3 cut(s)	1514, 1529, 1679
BmeT110I	CYCGRG	1 cut(s)	2044
BmiI	GGNNCC	9 cut(s)	194, 195, 210, 887, 953, 1079, 1249, 1809, 2146
BmrI	ACTGGG	1 cut(s)	1102
BmuI	ACTGGG	1 cut(s)	1102
BpiI	GAAGAC	1 cut(s)	583
Bpu14I	TTCGAA	2 cut(s)	391, 1109
BpuMI	CCSGG	1 cut(s)	2187
Bsa29I	ATCGAT	1 cut(s)	1646
BsaHI	GRCGYC	1 cut(s)	580
BsaI	GGTCTC	1 cut(s)	718
BsaJI	CCNNGG	9 cut(s)	272, 922, 1168, 1284, 1541, 1771, 2165, 2186, 2240
Bse118I	RCCGGY	2 cut(s)	205, 1436
Bse1I	ACTGG	7 cut(s)	595, 913, 953, 1097, 1420, 1495, 1502
Bse21I	CCTNAGG	1 cut(s)	2136
Bse3DI	GCAATG	2 cut(s)	1375, 2003
BseCI	ATCGAT	1 cut(s)	1646
BseDI	CCNNGG	9 cut(s)	272, 922, 1168, 1284, 1541, 1771, 2165, 2186, 2240
BseMI	GCAATG	2 cut(s)	1375, 2003
BseMII	CTCAG	3 cut(s)	492, 1721, 2150
BseNI	ACTGG	7 cut(s)	595, 913, 953, 1097, 1420, 1495, 1502
BseRI	GAGGAG	3 cut(s)	273, 1653, 1656
BseSI	GKGCMC	1 cut(s)	197
BsgI	GTGCAG	1 cut(s)	1414
Bsh1236I	CGCG	3 cut(s)	405, 804, 1170
Bsh1285I	CGRYCG	1 cut(s)	1880
BshNI	GGYRCC	4 cut(s)	885, 951, 1807, 2144
BshVI	ATCGAT	1 cut(s)	1646
BsiEI	CGRYCG	1 cut(s)	1880
BsiHKAI	GWGCWC	3 cut(s)	1065, 1663, 2125
BsiHKCI	CYCGRG	1 cut(s)	2044
BsiSI	CCGG	3 cut(s)	206, 1437, 2186
BslFI	GGGAC	1 cut(s)	887
BsmAI	GTCTC	1 cut(s)	718
BsmFI	GGGAC	1 cut(s)	887
Bso31I	GGTCTC	1 cut(s)	718
BsoBI	CYCGRG	1 cut(s)	2044
Bsp119I	TTCGAA	2 cut(s)	391, 1109
Bsp120I	GGGCCC	1 cut(s)	193
Bsp1286I	GDGCHC	5 cut(s)	197, 279, 1065, 1663, 2125
Bsp1407I	TGTACA	1 cut(s)	514
Bsp143I	GATC	9 cut(s)	13, 664, 1114, 1247, 1309, 1324, 1372, 1925, 2048
Bsp19I	CCATGG	1 cut(s)	922
Bsp68I	TCGCGA	1 cut(s)	804
BspCNI	CTCAG	3 cut(s)	491, 1720, 2149
BspDI	ATCGAT	1 cut(s)	1646
BspFNI	CGCG	3 cut(s)	405, 804, 1170
BspHI	TCATGA	3 cut(s)	661, 1213, 1420
BspLI	GGNNCC	9 cut(s)	194, 195, 210, 887, 953, 1079, 1249, 1809, 2146
BspMAI	CTGCAG	3 cut(s)	445, 1361, 1833
BspMI	ACCTGC	1 cut(s)	448
BspPI	GGATC	7 cut(s)	8, 1122, 1242, 1255, 1332, 1933, 2056
BspQI	GCTCTTC	1 cut(s)	888
BspT104I	TTCGAA	2 cut(s)	391, 1109
BspT107I	GGYRCC	4 cut(s)	885, 951, 1807, 2144
BspTI	CTTAAG	1 cut(s)	808
BspTNI	GGTCTC	1 cut(s)	718
BsrDI	GCAATG	2 cut(s)	1375, 2003
BsrFI	RCCGGY	2 cut(s)	205, 1436
BsrGI	TGTACA	1 cut(s)	514
BsrI	ACTGG	7 cut(s)	595, 913, 953, 1097, 1420, 1495, 1502
BssAI	RCCGGY	2 cut(s)	205, 1436
BssECI	CCNNGG	9 cut(s)	272, 922, 1168, 1284, 1541, 1771, 2165, 2186, 2240
BssMI	GATC	9 cut(s)	13, 664, 1114, 1247, 1309, 1324, 1372, 1925, 2048
BssNI	GRCGYC	1 cut(s)	580
BssT1I	CCWWGG	2 cut(s)	922, 1771
Bst4CI	ACNGT	7 cut(s)	308, 370, 424, 862, 885, 992, 1316
Bst6I	CTCTTC	2 cut(s)	888, 1698
BstACI	GRCGYC	1 cut(s)	580
BstAFI	CTTAAG	1 cut(s)	808
BstAPI	GCANNNNNTGC	4 cut(s)	67, 97, 121, 1789
BstAUI	TGTACA	1 cut(s)	514
BstBI	TTCGAA	2 cut(s)	391, 1109
BstC8I	GCNNGC	4 cut(s)	197, 279, 919, 1172
BstDEI	CTNAG	5 cut(s)	478, 1014, 1707, 2136, 2268
BstDSI	CCRYGG	2 cut(s)	922, 1168
BstEII	GGTNACC	2 cut(s)	1775, 1997
BstENI	CCTNNNNNAGG	1 cut(s)	2238
BstFNI	CGCG	3 cut(s)	405, 804, 1170
BstHHI	GCGC	2 cut(s)	405, 900
BstKTI	GATC	9 cut(s)	16, 667, 1117, 1250, 1312, 1327, 1375, 1928, 2051
BstMAI	GTCTC	1 cut(s)	718
BstMBI	GATC	9 cut(s)	13, 664, 1114, 1247, 1309, 1324, 1372, 1925, 2048
BstMCI	CGRYCG	1 cut(s)	1880
BstNSI	RCATGY	1 cut(s)	2098
BstPI	GGTNACC	2 cut(s)	1775, 1997
BstSFI	CTRYAG	4 cut(s)	441, 570, 1357, 1829
BstSLI	GKGCMC	1 cut(s)	197
BstUI	CGCG	3 cut(s)	405, 804, 1170
BstV2I	GAAGAC	1 cut(s)	583
BstX2I	RGATCY	4 cut(s)	1247, 1324, 1925, 2048
BstYI	RGATCY	4 cut(s)	1247, 1324, 1925, 2048
Bsu15I	ATCGAT	1 cut(s)	1646
Bsu36I	CCTNAGG	1 cut(s)	2136
BsuI	GTATCC	1 cut(s)	2142
BsuTUI	ATCGAT	1 cut(s)	1646
BtgI	CCRYGG	2 cut(s)	922, 1168
BtgZI	GCGATG	1 cut(s)	420
BtsI	GCAGTG	1 cut(s)	1366
BtsIMutI	CAGTG	3 cut(s)	366, 1321, 1366
BtuMI	TCGCGA	1 cut(s)	804
BveI	ACCTGC	1 cut(s)	448
Cac8I	GCNNGC	4 cut(s)	197, 279, 919, 1172
CaiI	CAGNNNCTG	2 cut(s)	1508, 1922
CciI	TCATGA	3 cut(s)	661, 1213, 1420
CfoI	GCGC	2 cut(s)	405, 900
Cfr10I	RCCGGY	2 cut(s)	205, 1436
Cfr42I	CCGCGG	1 cut(s)	1171
ClaI	ATCGAT	1 cut(s)	1646
CseI	GACGC	1 cut(s)	209
Csp6I	GTAC	4 cut(s)	7, 515, 1505, 1980
CspCI	CAANNNNNGTGG	2 cut(s)	481, 516
CviQI	GTAC	4 cut(s)	7, 515, 1505, 1980
DdeI	CTNAG	5 cut(s)	478, 1014, 1707, 2136, 2268
DpnI	GATC	9 cut(s)	15, 666, 1116, 1249, 1311, 1326, 1374, 1927, 2050
DpnII	GATC	9 cut(s)	13, 664, 1114, 1247, 1309, 1324, 1372, 1925, 2048
DraI	TTTAAA	1 cut(s)	528
DraIII	CACNNNGTG	1 cut(s)	1318
EaeI	YGGCCR	2 cut(s)	1045, 1438
Eam1104I	CTCTTC	2 cut(s)	888, 1698
EarI	CTCTTC	2 cut(s)	888, 1698
Ecl136II	GAGCTC	1 cut(s)	2123
Eco130I	CCWWGG	2 cut(s)	922, 1771
Eco24I	GRGCYC	3 cut(s)	197, 279, 2125
Eco31I	GGTCTC	1 cut(s)	718
Eco47I	GGWCC	3 cut(s)	1514, 1529, 1679
Eco53kI	GAGCTC	1 cut(s)	2123
Eco57I	CTGAAG	3 cut(s)	638, 1530, 1899
Eco81I	CCTNAGG	1 cut(s)	2136
Eco88I	CYCGRG	1 cut(s)	2044
Eco91I	GGTNACC	2 cut(s)	1775, 1997
EcoICRI	GAGCTC	1 cut(s)	2123
EcoNI	CCTNNNNNAGG	1 cut(s)	2238
EcoO109I	RGGNCCY	2 cut(s)	193, 1919
EcoO65I	GGTNACC	2 cut(s)	1775, 1997
EcoRI	GAATTC	2 cut(s)	608, 1971
EcoT14I	CCWWGG	2 cut(s)	922, 1771
EcoT22I	ATGCAT	2 cut(s)	1609, 2184
EcoT38I	GRGCYC	3 cut(s)	197, 279, 2125
ErhI	CCWWGG	2 cut(s)	922, 1771
FalI	AAGNNNNNCTT	4 cut(s)	812, 844, 978, 1010
FaqI	GGGAC	1 cut(s)	887
FauI	CCCGC	5 cut(s)	54, 204, 1163, 1680, 1788
FbaI	TGATCA	3 cut(s)	664, 1309, 1372
FriOI	GRGCYC	3 cut(s)	197, 279, 2125
FspBI	CTAG	4 cut(s)	363, 495, 971, 2208
GlaI	GCGC	2 cut(s)	404, 899
HapII	CCGG	3 cut(s)	206, 1437, 2186
HgaI	GACGC	1 cut(s)	209
HhaI	GCGC	2 cut(s)	405, 900
Hin1I	GRCGYC	1 cut(s)	580
Hin6I	GCGC	2 cut(s)	403, 898
HinP1I	GCGC	2 cut(s)	403, 898
HincII	GTYRAC	2 cut(s)	946, 1548
HindII	GTYRAC	2 cut(s)	946, 1548
HindIII	AAGCTT	1 cut(s)	332
HpaII	CCGG	3 cut(s)	206, 1437, 2186
HphI	GGTGA	4 cut(s)	731, 1469, 1769, 1991
Hpy166II	GTNNAC	6 cut(s)	517, 564, 946, 1132, 1548, 1682
Hpy188I	TCNGA	5 cut(s)	481, 546, 850, 1992, 2157
Hpy188III	TCNNGA	6 cut(s)	662, 803, 1214, 1421, 1601, 2046
Hpy8I	GTNNAC	6 cut(s)	517, 564, 946, 1132, 1548, 1682
Hpy99I	CGWCG	1 cut(s)	232
HpyAV	CCTTC	7 cut(s)	1105, 1144, 1174, 1505, 1553, 1944, 2158
HpyCH4III	ACNGT	7 cut(s)	308, 370, 424, 862, 885, 992, 1316
HpyCH4IV	ACGT	1 cut(s)	580
HpyF3I	CTNAG	5 cut(s)	478, 1014, 1707, 2136, 2268
HpySE526I	ACGT	1 cut(s)	580
Hsp92I	GRCGYC	1 cut(s)	580
HspAI	GCGC	2 cut(s)	403, 898
Ksp22I	TGATCA	3 cut(s)	664, 1309, 1372
KspI	CCGCGG	1 cut(s)	1171
Kzo9I	GATC	9 cut(s)	13, 664, 1114, 1247, 1309, 1324, 1372, 1925, 2048
LguI	GCTCTTC	1 cut(s)	888
LmnI	GCTCC	5 cut(s)	286, 851, 1621, 1666, 2120
MaeI	CTAG	4 cut(s)	363, 495, 971, 2208
MaeII	ACGT	1 cut(s)	580
MaeIII	GTNAC	4 cut(s)	1088, 1186, 1775, 1997
MalI	GATC	9 cut(s)	15, 666, 1116, 1249, 1311, 1326, 1374, 1927, 2050
MboI	GATC	9 cut(s)	13, 664, 1114, 1247, 1309, 1324, 1372, 1925, 2048
MboII	GAAGA	8 cut(s)	228, 588, 644, 905, 1229, 1343, 1685, 1905
MfeI	CAATTG	2 cut(s)	450, 703
MflI	RGATCY	4 cut(s)	1247, 1324, 1925, 2048
MhlI	GDGCHC	5 cut(s)	197, 279, 1065, 1663, 2125
MlsI	TGGCCA	1 cut(s)	1047
MluCI	AATT	9 cut(s)	182, 450, 608, 703, 833, 1293, 1495, 1971, 2172
MluNI	TGGCCA	1 cut(s)	1047
MlyI	GAGTC	5 cut(s)	559, 700, 1543, 1699, 2167
MmeI	TCCRAC	1 cut(s)	1558
Mox20I	TGGCCA	1 cut(s)	1047
Mph1103I	ATGCAT	2 cut(s)	1609, 2184
MroXI	GAANNNNTTC	2 cut(s)	648, 798
MscI	TGGCCA	1 cut(s)	1047
MseI	TTAA	7 cut(s)	527, 549, 636, 809, 864, 999, 1071
MslI	CAYNNNNRTG	1 cut(s)	371
Msp20I	TGGCCA	1 cut(s)	1047
MspA1I	CMGCKG	1 cut(s)	1170
MspCI	CTTAAG	1 cut(s)	808
MspI	CCGG	3 cut(s)	206, 1437, 2186
MunI	CAATTG	2 cut(s)	450, 703
MvnI	CGCG	3 cut(s)	405, 804, 1170
NciI	CCSGG	1 cut(s)	2187
NcoI	CCATGG	1 cut(s)	922
NdeII	GATC	9 cut(s)	13, 664, 1114, 1247, 1309, 1324, 1372, 1925, 2048
NlaIV	GGNNCC	9 cut(s)	194, 195, 210, 887, 953, 1079, 1249, 1809, 2146
NmuCI	GTSAC	3 cut(s)	1186, 1775, 1997
NruI	TCGCGA	1 cut(s)	804
NsiI	ATGCAT	2 cut(s)	1609, 2184
NspI	RCATGY	1 cut(s)	2098
NspV	TTCGAA	2 cut(s)	391, 1109
OliI	CACNNNNGTG	1 cut(s)	371
PagI	TCATGA	3 cut(s)	661, 1213, 1420
PaqCI	CACCTGC	1 cut(s)	448
PciSI	GCTCTTC	1 cut(s)	888
PdmI	GAANNNNTTC	2 cut(s)	648, 798
PfeI	GAWTC	5 cut(s)	388, 535, 649, 794, 1987
PflMI	CCANNNNNTGG	2 cut(s)	1526, 1577
PfoI	TCCNGGA	2 cut(s)	313, 1525
PleI	GAGTC	5 cut(s)	559, 699, 1543, 1699, 2166
PpsI	GAGTC	5 cut(s)	559, 699, 1543, 1699, 2166
PshBI	ATTAAT	1 cut(s)	549
Psp124BI	GAGCTC	1 cut(s)	2125
PspEI	GGTNACC	2 cut(s)	1775, 1997
PspN4I	GGNNCC	9 cut(s)	194, 195, 210, 887, 953, 1079, 1249, 1809, 2146
PspOMI	GGGCCC	1 cut(s)	193
PstI	CTGCAG	3 cut(s)	445, 1361, 1833
PstNI	CAGNNNCTG	2 cut(s)	1508, 1922
PsuI	RGATCY	4 cut(s)	1247, 1324, 1925, 2048
RruI	TCGCGA	1 cut(s)	804
RsaI	GTAC	4 cut(s)	8, 516, 1506, 1981
RsaNI	GTAC	4 cut(s)	7, 515, 1505, 1980
RseI	CAYNNNNRTG	1 cut(s)	371
SacI	GAGCTC	1 cut(s)	2125
SacII	CCGCGG	1 cut(s)	1171
SapI	GCTCTTC	1 cut(s)	888
SaqAI	TTAA	7 cut(s)	527, 549, 636, 809, 864, 999, 1071
Sau3AI	GATC	9 cut(s)	13, 664, 1114, 1247, 1309, 1324, 1372, 1925, 2048
SchI	GAGTC	5 cut(s)	559, 700, 1543, 1699, 2167
SduI	GDGCHC	5 cut(s)	197, 279, 1065, 1663, 2125
SfcI	CTRYAG	4 cut(s)	441, 570, 1357, 1829
Sfr303I	CCGCGG	1 cut(s)	1171
SfuI	TTCGAA	2 cut(s)	391, 1109
SgrBI	CCGCGG	1 cut(s)	1171
SinI	GGWCC	3 cut(s)	1514, 1529, 1679
SmiMI	CAYNNNNRTG	1 cut(s)	371
SmlI	CTYRAG	1 cut(s)	808
SmoI	CTYRAG	1 cut(s)	808
SpeI	ACTAGT	1 cut(s)	970
Sse9I	AATT	9 cut(s)	182, 450, 608, 703, 833, 1293, 1495, 1971, 2172
SspI	AATATT	1 cut(s)	1223
SspMI	CTAG	4 cut(s)	363, 495, 971, 2208
SstI	GAGCTC	1 cut(s)	2125
StyI	CCWWGG	2 cut(s)	922, 1771
TaaI	ACNGT	7 cut(s)	308, 370, 424, 862, 885, 992, 1316
TaiI	ACGT	1 cut(s)	583
TaqI	TCGA	6 cut(s)	391, 1109, 1177, 1552, 1646, 1876
TasI	AATT	9 cut(s)	182, 450, 608, 703, 833, 1293, 1495, 1971, 2172
TatI	WGTACW	1 cut(s)	514
TauI	GCSGC	1 cut(s)	1861
TfiI	GAWTC	5 cut(s)	388, 535, 649, 794, 1987
Tru1I	TTAA	7 cut(s)	527, 549, 636, 809, 864, 999, 1071
Tru9I	TTAA	7 cut(s)	527, 549, 636, 809, 864, 999, 1071
TscAI	CASTG	3 cut(s)	373, 1321, 1366
TseFI	GTSAC	3 cut(s)	1186, 1775, 1997
Tsp45I	GTSAC	3 cut(s)	1186, 1775, 1997
TspGWI	ACGGA	1 cut(s)	1197
TspRI	CASTG	3 cut(s)	373, 1321, 1366
Van91I	CCANNNNNTGG	2 cut(s)	1526, 1577
Vha464I	CTTAAG	1 cut(s)	808
VpaK11BI	GGWCC	3 cut(s)	1514, 1529, 1679
VspI	ATTAAT	1 cut(s)	549
XagI	CCTNNNNNAGG	1 cut(s)	2238
XapI	RAATTY	6 cut(s)	182, 608, 833, 1293, 1971, 2172
XceI	RCATGY	1 cut(s)	2098
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1265
XmnI	GAANNNNTTC	2 cut(s)	648, 798
XspI	CTAG	4 cut(s)	363, 495, 971, 2208
ZraI	GACGTC	1 cut(s)	581
Zsp2I	ATGCAT	2 cut(s)	1609, 2184

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.