Rh5DG078600

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000356
Physical Location & Seq
Forward (+)
6886424 .. 6888323
1900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG078600.1

Sequence Viewer

Length: 1341 bp
ATGGTTCCGGAGTATCATCCCCACCCCTACGCCCCTGCTGGTCCTCATGCTCCTCCTCATGTCGAAGGCTTTGGTGCAATAAGAAACAGAAAGCAGACCCAGGGAAGGACAGTTGATTACACTACCTCTGTCGTGCAATACACACAGACTCGAAAGCGGCAGCGTGATGCAAGAGATACAACAGTGTTGCAACCTACACCAGCAGCATCAATCAATTCGTTGCCGCCGGTTGCGTATTCGGATAACCCATCTAGGAGCTTTGCTGCAAAGTTTGTGCATGCTTGTACAAATTACAGAGCCCGTACTTGTCCGATTAATCGGGTTTTGTGGACACCTTCCGGGAGGCGTCTCATTACAGGGTCCCAAAATGGGGAGTTCACTCTTTGGGATGGTCAGTCATTTAACTATGAATTGAGTTTTCTGGCTCATGATCAAGCAGTCAGGTCTATGGCGTGGAGTTATGATGGTGAGAATTGGATTTCTGGTGATGATGGGGGATCAATAAAGTATTGGACGAGTAACATGAACAATGTGCTAGTCAATGAATCTGCTCACCGAGAATCGGTTCGGGACTTGAGCTTTTGTAGGACTAATTTGAAGTTCTGTTCATGTTCGGATGATACTTATGTGAAAATCTGGGATTATGAAAGGTGCCAAGAAGAGTTCAGATTGACTGGCCATGGTTGGAATGTGAAGAGTGTTGACTGGCACCCGACAAAGTCCTTACTAGCTTCAGGTGGGAAGGACAGTGTTGTCAAACTGTGGGATGCTAGGACAGGGAGAGAGCTTTGTTCATTTCATGATCACAAAAATTGGGTGCAATCTGTTAAGTGGAGCCGAAATGGTAACTGGCTGCTAACTGCTTCCAAGGATCAAGTCATTAAGCTTTACGACATGAGGGCTATGAAGGAACTTGAATCATTCCACGGGCATCGGAATGAAGTGACTGCTATAGCTTGGCATCCTTTTCATGAAGAATATTTTGTCAGTGGGAGTAGTGATGGATCCATTTTCCATTGGCTTGTTGGGCATGAAACTCCCCAGGTTGAAGTTCCTAATGCACACAGTAACAATCATAATAACAGTGTGTGGGATCTCCAATGGCATCCTATTGGTCATATGCTTTGCAGTGGTAGCAATGATCGCACAACAAAGTTTTGGTGCAGAAATAGGCCAGGAGATAAATGTAACATCAGTCAGAATCAAAGTATTGGTGATCGAAGTTCTGCTTTTGCTGGTCACATGACTGGTAACTTTCCATTTCCATTACATGAAGGACAACCAAAAATTGCTACTCGAAACCAAGGAGCCATTATTCCAGGTGTTGGATTTGCAATTTAA

Protein Analysis

446

Amino Acids

50.62

Weight (kDa)

8.46

Isoelectric Point (pI)

35.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 89 - 216 4.6e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 96 - 219 3.1e-16 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 96 - 273 1.2e-28 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 97 - 265 7.3e-24 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 99 - 312 5.8e-20 G protein beta WD-40 repeat protein
Beta-prop_IFT140_1st PF23383 100 - 189 2e-08 IFT140 first beta-propeller
Beta-prop_WDR3_1st PF25173 102 - 216 2.1e-17 WDR3 first beta-propeller domain
Beta-prop_EIPR1 PF23609 106 - 255 1.5e-06 EIPR1 beta-propeller
WD40_WDHD1_1st PF24817 107 - 264 7.9e-16 WDHD1 first WD40 domain
WD40_Prp19 PF24814 113 - 307 3.6e-31 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 134 - 256 1.3e-13 WDR3 second beta-propeller domain
EIF3I PF24805 139 - 218 3.1e-07 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 142 - 250 4.1e-06 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 175 - 340 2.5e-17 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 180 - 340 1.8e-17 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 184 - 277 1.8e-19 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 197 - 341 5.6e-22 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 201 - 288 4.9e-08 WDR90/POC16, second beta-propeller
Beta-prop_TEP1_2nd PF25047 203 - 319 8.4e-13 TEP-1 second beta-propeller
Beta-prop_SCAP PF24017 203 - 298 4.3e-06 SCAP Beta-propeller
Beta-prop_WDR3_1st PF25173 212 - 340 9.2e-28 WDR3 first beta-propeller domain
WD40 PF00400 222 - 256 1.3e-08 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 223 - 343 1e-12 WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 224 - 305 2.6e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML PF23409 225 - 330 3.9e-09 Echinoderm microtubule-associated protein first beta-propeller
EIF3I PF24805 225 - 386 4.6e-14 EIF3I
Beta-prop_IFT140_1st PF23383 227 - 296 9.5e-07 IFT140 first beta-propeller
Beta-prop_Aladin PF25460 229 - 338 2.1e-07 Aladin seven-bladed propeller
WD40_MABP1-WDR62_2nd PF24782 231 - 387 1.6e-09 MABP1/WDR62 second WD40 domain
Beta-prop_WDR19_1st PF23389 234 - 384 2.1e-07 WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 247 - 388 3.8e-10 WDR36/Utp21 first beta-propeller
Beta-prop_EIPR1 PF23609 258 - 387 1.6e-07 EIPR1 beta-propeller
WD40_CDC20-Fz PF24807 261 - 391 1e-21 CDC20/Fizzy WD40 domain
WD40 PF00400 261 - 298 5e-08 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 267 - 389 1.3e-19 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 269 - 387 2.3e-21 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 273 - 389 6.1e-13 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 285 - 380 8.3e-07 WDR90/POC16, second beta-propeller
WD40 PF00400 303 - 340 6.9e-06 WD domain, G-beta repeat
WD40_Prp19 PF24814 304 - 387 8.5e-10 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica Prupe.2G034300_v2.0.a1
pyrus_communis pycom02g24420 pycom07g02650
rosa_chinensis RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 752
AccB1I GGYRCC 2 cut(s) 651, 708
AccB7I CCANNNNNTGG 1 cut(s) 1325
AccIII TCCGGA 1 cut(s) 7
AciI CCGC 2 cut(s) 157, 224
AclWI GGATC 5 cut(s) 505, 879, 999, 1012, 1101
AcoI YGGCCR 1 cut(s) 676
AcuI CTGAAG 1 cut(s) 717
AcyI GRCGYC 1 cut(s) 346
AfaI GTAC 2 cut(s) 286, 304
AfiI CCNNNNNNNGG 5 cut(s) 105, 369, 370, 562, 1325
AgsI TTSAA 3 cut(s) 598, 917, 1049
AjnI CCWGG 4 cut(s) 99, 1041, 1174, 1318
AluBI AGCT 6 cut(s) 258, 579, 731, 787, 886, 956
AluI AGCT 6 cut(s) 258, 579, 731, 787, 886, 956
Alw26I GTCTC 1 cut(s) 353
AlwI GGATC 5 cut(s) 505, 879, 999, 1012, 1101
Aor13HI TCCGGA 1 cut(s) 7
AoxI GGCC 2 cut(s) 676, 1172
ApeKI GCWGC 4 cut(s) 160, 203, 263, 853
AseI ATTAAT 1 cut(s) 315
Asp700I GAANNNNTTC 1 cut(s) 564
AspS9I GGNCC 2 cut(s) 41, 360
AsuC2I CCSGG 1 cut(s) 340
AsuHPI GGTGA 4 cut(s) 479, 497, 545, 1226
AvaII GGWCC 2 cut(s) 41, 360
BaeI ACNNNNGTAYC 4 cut(s) 168, 201, 612, 645
BalI TGGCCA 1 cut(s) 678
BamHI GGATCC 1 cut(s) 1004
BanI GGYRCC 2 cut(s) 651, 708
BanII GRGCYC 1 cut(s) 301
BbvI GCAGC 4 cut(s) 172, 215, 250, 840
BccI CCATC 5 cut(s) 256, 383, 458, 485, 995
BciT130I CCWGG 4 cut(s) 101, 1043, 1176, 1320
BclI TGATCA 2 cut(s) 430, 802
BcnI CCSGG 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 353
BfaI CTAG 4 cut(s) 252, 536, 728, 771
BfmI CTRYAG 1 cut(s) 951
BisI GCNGC 6 cut(s) 158, 161, 204, 224, 264, 854
BlsI GCNGC 6 cut(s) 159, 162, 205, 225, 265, 855
Bme1390I CCNGG 5 cut(s) 101, 340, 1043, 1176, 1320
Bme18I GGWCC 2 cut(s) 41, 360
BmgT120I GGNCC 2 cut(s) 41, 360
BmiI GGNNCC 8 cut(s) 6, 361, 362, 653, 710, 836, 1006, 1309
BmrFI CCNGG 5 cut(s) 101, 340, 1043, 1176, 1320
BmsI GCATC 6 cut(s) 157, 215, 757, 940, 970, 1114
BpuEI CTTGAG 1 cut(s) 595
BpuMI CCSGG 1 cut(s) 340
BsaHI GRCGYC 1 cut(s) 346
BsaJI CCNNGG 7 cut(s) 99, 100, 679, 867, 925, 1041, 1303
BsaWI WCCGGW 1 cut(s) 7
Bsc4I CCNNNNNNNGG 5 cut(s) 105, 369, 370, 562, 1325
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 4 cut(s) 679, 710, 854, 1252
Bse3DI GCAATG 1 cut(s) 1144
BseAI TCCGGA 1 cut(s) 7
BseBI CCWGG 4 cut(s) 101, 1043, 1176, 1320
BseDI CCNNGG 7 cut(s) 99, 100, 679, 867, 925, 1041, 1303
BseGI GGATG 6 cut(s) 16, 394, 622, 772, 961, 1105
BseLI CCNNNNNNNGG 5 cut(s) 105, 369, 370, 562, 1325
BseMI GCAATG 1 cut(s) 1144
BseNI ACTGG 4 cut(s) 679, 710, 854, 1252
BseRI GAGGAG 2 cut(s) 42, 45
BseXI GCAGC 4 cut(s) 172, 215, 250, 840
BsgI GTGCAG 1 cut(s) 1183
BshFI GGCC 2 cut(s) 678, 1174
BshNI GGYRCC 2 cut(s) 651, 708
BsiSI CCGG 3 cut(s) 8, 227, 339
BslFI GGGAC 2 cut(s) 346, 584
BslI CCNNNNNNNGG 5 cut(s) 105, 369, 370, 562, 1325
BsmAI GTCTC 1 cut(s) 353
BsmBI CGTCTC 1 cut(s) 353
BsmFI GGGAC 2 cut(s) 346, 584
BsnI GGCC 2 cut(s) 678, 1174
Bsp1286I GDGCHC 1 cut(s) 301
Bsp13I TCCGGA 1 cut(s) 7
Bsp1407I TGTACA 1 cut(s) 284
Bsp143I GATC 8 cut(s) 430, 497, 802, 871, 1004, 1093, 1141, 1216
Bsp19I CCATGG 1 cut(s) 679
BspACI CCGC 2 cut(s) 157, 224
BspANI GGCC 2 cut(s) 678, 1174
BspEI TCCGGA 1 cut(s) 7
BspHI TCATGA 3 cut(s) 427, 799, 970
BspLI GGNNCC 8 cut(s) 6, 361, 362, 653, 710, 836, 1006, 1309
BspPI GGATC 5 cut(s) 505, 879, 999, 1012, 1101
BspT107I GGYRCC 2 cut(s) 651, 708
BsrDI GCAATG 1 cut(s) 1144
BsrFI RCCGGY 1 cut(s) 226
BsrGI TGTACA 1 cut(s) 284
BsrI ACTGG 4 cut(s) 679, 710, 854, 1252
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 7 cut(s) 99, 100, 679, 867, 925, 1041, 1303
BssMI GATC 8 cut(s) 430, 497, 802, 871, 1004, 1093, 1141, 1216
BssNI GRCGYC 1 cut(s) 346
BssT1I CCWWGG 3 cut(s) 679, 867, 1303
Bst2UI CCWGG 4 cut(s) 101, 1043, 1176, 1320
Bst4CI ACNGT 6 cut(s) 112, 184, 749, 762, 1067, 1085
Bst6I CTCTTC 2 cut(s) 654, 689
BstACI GRCGYC 1 cut(s) 346
BstAUI TGTACA 1 cut(s) 284
BstC8I GCNNGC 1 cut(s) 279
BstDSI CCRYGG 2 cut(s) 679, 925
BstF5I GGATG 6 cut(s) 16, 394, 622, 772, 961, 1105
BstKTI GATC 8 cut(s) 433, 500, 805, 874, 1007, 1096, 1144, 1219
BstMAI GTCTC 1 cut(s) 353
BstMBI GATC 8 cut(s) 430, 497, 802, 871, 1004, 1093, 1141, 1216
BstMWI GCNNNNNNNGC 3 cut(s) 1027, 1134, 1143
BstNI CCWGG 4 cut(s) 101, 1043, 1176, 1320
BstNSI RCATGY 1 cut(s) 281
BstSCI CCNGG 5 cut(s) 99, 338, 1041, 1174, 1318
BstSFI CTRYAG 1 cut(s) 951
BstV1I GCAGC 4 cut(s) 172, 215, 250, 840
BstX2I RGATCY 2 cut(s) 1004, 1093
BstYI RGATCY 2 cut(s) 1004, 1093
BsuRI GGCC 2 cut(s) 678, 1174
BtgI CCRYGG 2 cut(s) 679, 925
BtsCI GGATG 6 cut(s) 16, 394, 622, 772, 961, 1105
BtsI GCAGTG 1 cut(s) 1135
BtsIMutI CAGTG 5 cut(s) 189, 754, 994, 1090, 1135
Cac8I GCNNGC 1 cut(s) 279
CciI TCATGA 3 cut(s) 427, 799, 970
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 2 cut(s) 41, 360
CseI GACGC 1 cut(s) 335
Csp6I GTAC 2 cut(s) 285, 303
CviQI GTAC 2 cut(s) 285, 303
DpnI GATC 8 cut(s) 432, 499, 804, 873, 1006, 1095, 1143, 1218
DpnII GATC 8 cut(s) 430, 497, 802, 871, 1004, 1093, 1141, 1216
DrdI GACNNNNNNGTC 1 cut(s) 752
DseDI GACNNNNNNGTC 1 cut(s) 752
EaeI YGGCCR 1 cut(s) 676
Eam1104I CTCTTC 2 cut(s) 654, 689
EarI CTCTTC 2 cut(s) 654, 689
Eco130I CCWWGG 3 cut(s) 679, 867, 1303
Eco24I GRGCYC 1 cut(s) 301
Eco47I GGWCC 2 cut(s) 41, 360
Eco57I CTGAAG 1 cut(s) 717
EcoO109I RGGNCCY 1 cut(s) 360
EcoRII CCWGG 4 cut(s) 99, 1041, 1174, 1318
EcoT14I CCWWGG 3 cut(s) 679, 867, 1303
EcoT38I GRGCYC 1 cut(s) 301
ErhI CCWWGG 3 cut(s) 679, 867, 1303
Esp3I CGTCTC 1 cut(s) 353
FalI AAGNNNNNCTT 2 cut(s) 1213, 1245
FaqI GGGAC 2 cut(s) 346, 584
FauNDI CATATG 1 cut(s) 1119
FbaI TGATCA 2 cut(s) 430, 802
Fnu4HI GCNGC 6 cut(s) 158, 161, 204, 224, 264, 854
FokI GGATG 6 cut(s) 3, 401, 629, 779, 948, 1092
FriOI GRGCYC 1 cut(s) 301
Fsp4HI GCNGC 6 cut(s) 158, 161, 204, 224, 264, 854
FspBI CTAG 4 cut(s) 252, 536, 728, 771
GluI GCNGC 6 cut(s) 158, 161, 204, 224, 264, 854
HaeIII GGCC 2 cut(s) 678, 1174
HapII CCGG 3 cut(s) 8, 227, 339
HgaI GACGC 1 cut(s) 335
Hin1I GRCGYC 1 cut(s) 346
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HindIII AAGCTT 1 cut(s) 884
HinfI GANTC 5 cut(s) 148, 545, 560, 917, 1201
HpaII CCGG 3 cut(s) 8, 227, 339
HphI GGTGA 4 cut(s) 479, 497, 545, 1226
Hpy166II GTNNAC 3 cut(s) 330, 378, 703
Hpy188I TCNGA 6 cut(s) 241, 312, 616, 668, 936, 1200
Hpy188III TCNNGA 5 cut(s) 8, 428, 569, 800, 971
Hpy8I GTNNAC 3 cut(s) 330, 378, 703
HpyAV CCTTC 6 cut(s) 59, 99, 345, 736, 901, 1268
HpyCH4III ACNGT 6 cut(s) 112, 184, 749, 762, 1067, 1085
HpyF10VI GCNNNNNNNGC 3 cut(s) 1027, 1134, 1143
Hsp92I GRCGYC 1 cut(s) 346
KflI GGGWCCC 1 cut(s) 360
Kpn2I TCCGGA 1 cut(s) 7
Ksp22I TGATCA 2 cut(s) 430, 802
Kzo9I GATC 8 cut(s) 430, 497, 802, 871, 1004, 1093, 1141, 1216
LmnI GCTCC 4 cut(s) 55, 255, 834, 1307
Lsp1109I GCAGC 4 cut(s) 172, 215, 250, 840
LweI GCATC 6 cut(s) 157, 215, 757, 940, 970, 1114
MaeI CTAG 4 cut(s) 252, 536, 728, 771
MaeIII GTNAC 7 cut(s) 518, 845, 943, 1067, 1187, 1238, 1250
MalI GATC 8 cut(s) 432, 499, 804, 873, 1006, 1095, 1143, 1218
MboI GATC 8 cut(s) 430, 497, 802, 871, 1004, 1093, 1141, 1216
MboII GAAGA 3 cut(s) 671, 706, 986
MflI RGATCY 2 cut(s) 1004, 1093
MhlI GDGCHC 1 cut(s) 301
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 8 cut(s) 214, 289, 410, 472, 592, 811, 1287, 1335
MluNI TGGCCA 1 cut(s) 678
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 2 cut(s) 665, 1306
MnlI CCTC 6 cut(s) 54, 63, 66, 136, 336, 891
Mox20I TGGCCA 1 cut(s) 678
MroI TCCGGA 1 cut(s) 7
MroXI GAANNNNTTC 1 cut(s) 564
MscI TGGCCA 1 cut(s) 678
MseI TTAA 5 cut(s) 315, 402, 828, 882, 1339
MslI CAYNNNNRTG 1 cut(s) 936
Msp20I TGGCCA 1 cut(s) 678
MspI CCGG 3 cut(s) 8, 227, 339
MspR9I CCNGG 5 cut(s) 101, 340, 1043, 1176, 1320
MvaI CCWGG 4 cut(s) 101, 1043, 1176, 1320
MwoI GCNNNNNNNGC 3 cut(s) 1027, 1134, 1143
NciI CCSGG 1 cut(s) 340
NcoI CCATGG 1 cut(s) 679
NdeI CATATG 1 cut(s) 1119
NdeII GATC 8 cut(s) 430, 497, 802, 871, 1004, 1093, 1141, 1216
NlaIV GGNNCC 8 cut(s) 6, 361, 362, 653, 710, 836, 1006, 1309
NmuCI GTSAC 2 cut(s) 943, 1238
NspI RCATGY 1 cut(s) 281
PaeI GCATGC 1 cut(s) 281
PagI TCATGA 3 cut(s) 427, 799, 970
PasI CCCWGGG 1 cut(s) 100
PdmI GAANNNNTTC 1 cut(s) 564
PfeI GAWTC 4 cut(s) 545, 560, 917, 1201
PflFI GACNNNGTC 1 cut(s) 718
PflMI CCANNNNNTGG 1 cut(s) 1325
PfoI TCCNGGA 1 cut(s) 338
PkrI GCNGC 6 cut(s) 159, 162, 205, 225, 265, 855
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
PpuMI RGGWCCY 1 cut(s) 360
PshBI ATTAAT 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 360
Psp6I CCWGG 4 cut(s) 99, 1041, 1174, 1318
PspGI CCWGG 4 cut(s) 99, 1041, 1174, 1318
PspN4I GGNNCC 8 cut(s) 6, 361, 362, 653, 710, 836, 1006, 1309
PspPI GGNCC 2 cut(s) 41, 360
PspPPI RGGWCCY 1 cut(s) 360
PsuI RGATCY 2 cut(s) 1004, 1093
PsyI GACNNNGTC 1 cut(s) 718
RsaI GTAC 2 cut(s) 286, 304
RsaNI GTAC 2 cut(s) 285, 303
RseI CAYNNNNRTG 1 cut(s) 936
SaqAI TTAA 5 cut(s) 315, 402, 828, 882, 1339
SatI GCNGC 6 cut(s) 158, 161, 204, 224, 264, 854
Sau3AI GATC 8 cut(s) 430, 497, 802, 871, 1004, 1093, 1141, 1216
Sau96I GGNCC 2 cut(s) 41, 360
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 5 cut(s) 101, 340, 1043, 1176, 1320
SduI GDGCHC 1 cut(s) 301
SfaNI GCATC 6 cut(s) 157, 215, 757, 940, 970, 1114
SfcI CTRYAG 1 cut(s) 951
SinI GGWCC 2 cut(s) 41, 360
SmiMI CAYNNNNRTG 1 cut(s) 936
SmlI CTYRAG 1 cut(s) 574
SmoI CTYRAG 1 cut(s) 574
SphI GCATGC 1 cut(s) 281
Sse9I AATT 8 cut(s) 214, 289, 410, 472, 592, 811, 1287, 1335
SsiI CCGC 2 cut(s) 157, 224
SspI AATATT 1 cut(s) 980
SspMI CTAG 4 cut(s) 252, 536, 728, 771
StyD4I CCNGG 5 cut(s) 99, 338, 1041, 1174, 1318
StyI CCWWGG 3 cut(s) 679, 867, 1303
TaaI ACNGT 6 cut(s) 112, 184, 749, 762, 1067, 1085
TaqI TCGA 4 cut(s) 63, 151, 1219, 1297
TasI AATT 8 cut(s) 214, 289, 410, 472, 592, 811, 1287, 1335
TatI WGTACW 1 cut(s) 284
TauI GCSGC 2 cut(s) 160, 226
TfiI GAWTC 4 cut(s) 545, 560, 917, 1201
Tru1I TTAA 5 cut(s) 315, 402, 828, 882, 1339
Tru9I TTAA 5 cut(s) 315, 402, 828, 882, 1339
TscAI CASTG 5 cut(s) 189, 754, 994, 1090, 1135
TseFI GTSAC 2 cut(s) 943, 1238
TseI GCWGC 4 cut(s) 160, 203, 263, 853
Tsp45I GTSAC 2 cut(s) 943, 1238
TspRI CASTG 5 cut(s) 189, 754, 994, 1090, 1135
Tth111I GACNNNGTC 1 cut(s) 718
Van91I CCANNNNNTGG 1 cut(s) 1325
VpaK11BI GGWCC 2 cut(s) 41, 360
VspI ATTAAT 1 cut(s) 315
XceI RCATGY 1 cut(s) 281
XcmI CCANNNNNNNNNTGG 1 cut(s) 1022
XmnI GAANNNNTTC 1 cut(s) 564
XspI CTAG 4 cut(s) 252, 536, 728, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.