Rw3G022520

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000356
Physical Location & Seq
Reverse (-)
27621088 .. 27623060
1973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G022520.1

Sequence Viewer

Length: 1398 bp
ATGGTCCCAGAGTATCACTACCGCCCTCTCCTTCAACTAGTGAATATGGTCCCAGCGTATCACTACCGCCCCTATGCCCCTTGCGGTCCTCCTCATACCCAGATCAGAAGGACGATTGATTACACTGGCTCTGTCGTCCGATACACACAGAACCGAAAGTGGCGGCGTGATGCAATGGAACCAATCGATCTGTTGCCGCCAACTGCCTCTTCTTCGATTAATAACCCATTTGCTACACAGTTTGTGCACTCTTGTACAAATTACAGAGCCCGTGCTTCTTCGATTAATCGAGTTTTATGGACACCTTCTGGGAGCCGTCTTATTACAAGCTCCCAAAATGGGGAGTTCACTCTTTGGAATGGTCAGTCATTTAACCATGAATTGAGGATTCAGGCTCATGATCAACCAATCAGGTCCATGGCGTGGAGTTTTGATGATGAAGATTGGATCTCTGGTGATGATGGAGGCTCAATCAGGTATTGGAAGAGTAACATGAACAATGTGCTAGTCAATGAATCTGCTCACCAAGAATCGGTTCGGGACTTAAGCTTCTGTCGGAATAATTTGAAGTTTTGTTCATGTTCGGATGACACTACTGTTAAAATCTGGGATTTTGAACGGTGCCAACAAGAGCAGACATTGACCGGCCATGGTTGGAATGTCAAGAGTGTTGACTGGCACCCCACCAAGTCTCTACTAGCTTCAGGTGGGAAAGACAGTGTTGTCAAACTGTGGGATGCTAGGTCAGGGAGAGAACTTTGTTCATTTTATGATCACAAAAATTGGGTGCATTCTGTTAAGTGGAACCGAAATGGTAACTGGCTGCTAACTGCTTCCAAGGATCAAGTCATTAAGCTTTACAACTTGAGGGCTATGAAGGAACTTAGATCTTTCCACGGCCATCAGAACAAAGTGACGGCTCTAGCTTGGCATCCTCTTACTGAAGAATATTTTGTCAGTGGGACTAGGGATGGATCCATTTTGCATTGGCTTGTTGGGCATGAAACTCCCCAGGTTGAAATTCCTAATGCACACAGTAACAGTTACAATAATAGTGTGTGGGATCTCCAGTGGCATCCTATTGGTCATATGATTTGTAGTGGTAGCAATGATCACACCACAAAGTTTTGGTGCAGATCAGGAGATAAATCTAAATTCAGTCAGAATCAAAACCAAGGAACCATTGGTTCAAGAGGTGTTGGAACCATTATACCAGGTGTTGGAATTGCGTTTCCAGCCAGAAGAGACCAAGTTCAATTTTCCCACCCCACAAAAACTGGCTGTGAATCGATGTTGAATCAACCGTCTCAAATATCAAATTTTCCAAGGCCCCCATATGCTTTTAGTTACCAAAAGAGACCAAGGCCAAGTTCCCCACCCCACGGAAACTGGCTGTGA

Protein Analysis

465

Amino Acids

53.34

Weight (kDa)

9.14

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 82 - 253 3.5e-23 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 86 - 256 8e-27 THOC3 beta-propeller domain
WD40_Gbeta PF25391 88 - 298 6.3e-21 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 89 - 217 3.2e-16 WDR3 first beta-propeller domain
Beta-prop_IFT140_1st PF23383 90 - 180 8.5e-09 IFT140 first beta-propeller
Beta-prop_WDR5 PF25175 91 - 179 1.9e-07 WDR5 beta-propeller domain
Beta-prop_EIPR1 PF23609 94 - 245 7.8e-08 EIPR1 beta-propeller
WD40_Prp19 PF24814 94 - 297 1.8e-28 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 95 - 253 3.1e-16 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 108 - 378 6.9e-13 WDR36/Utp21 first beta-propeller
EIF3I PF24805 125 - 212 8.5e-09 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 131 - 240 1.3e-07 CAF1B/HIR1 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 137 - 304 3.4e-11 MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd PF25047 139 - 353 5.2e-20 TEP-1 second beta-propeller
WDR55 PF24796 165 - 293 1.1e-16 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 170 - 291 1.1e-15 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 173 - 279 2.7e-24 WDR5 beta-propeller domain
WD40 PF00400 173 - 204 3.2e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 174 - 247 1.8e-10 WDR3 second beta-propeller domain
Beta-prop_SCAP PF24017 174 - 268 3.4e-06 SCAP Beta-propeller
Beta-prop_EML_2 PF23414 187 - 332 2e-21 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 191 - 278 2.4e-08 WDR90/POC16, second beta-propeller
Beta-prop_IFT140_1st PF23383 198 - 286 2.4e-06 IFT140 first beta-propeller
Beta-prop_WDR3_1st PF25173 202 - 330 1.9e-26 WDR3 first beta-propeller domain
WD40 PF00400 209 - 246 1.7e-09 WD domain, G-beta repeat
EIF3I PF24805 213 - 376 5.2e-13 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 213 - 293 7.5e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML PF23409 215 - 320 2.8e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 221 - 385 1.8e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR19_1st PF23389 224 - 384 6.1e-06 WDR19 first beta-propeller
WD40_CDC20-Fz PF24807 251 - 385 5.8e-20 CDC20/Fizzy WD40 domain
WD40 PF00400 251 - 288 2.3e-07 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 253 - 380 2.9e-10 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 258 - 377 3.4e-17 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 259 - 389 4.4e-22 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 262 - 374 8.5e-08 Aladin seven-bladed propeller
WD40_WDHD1_1st PF24817 263 - 370 1e-11 WDHD1 first WD40 domain
WD40_Prp19 PF24814 295 - 377 4.7e-08 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica Prupe.2G034300_v2.0.a1
pyrus_communis pycom02g24420 pycom07g02650
rosa_chinensis RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 722
AccB1I GGYRCC 2 cut(s) 621, 678
AccB7I CCANNNNNTGG 2 cut(s) 423, 1220
AciI CCGC 5 cut(s) 22, 67, 84, 163, 197
AclWI GGATC 5 cut(s) 455, 849, 969, 982, 1071
AcoI YGGCCR 2 cut(s) 646, 898
AcsI RAATTY 3 cut(s) 1020, 1154, 1318
AcuI CTGAAG 2 cut(s) 687, 963
AfaI GTAC 1 cut(s) 256
AfiI CCNNNNNNNGG 6 cut(s) 339, 340, 423, 532, 1220, 1382
AflII CTTAAG 1 cut(s) 544
AgsI TTSAA 7 cut(s) 35, 568, 617, 1019, 1191, 1256, 1297
AhlI ACTAGT 1 cut(s) 37
AjnI CCWGG 2 cut(s) 1011, 1213
AjuI GAANNNNNNNTTGG 6 cut(s) 193, 225, 519, 551, 1354, 1386
AluBI AGCT 5 cut(s) 330, 549, 701, 856, 926
AluI AGCT 5 cut(s) 330, 549, 701, 856, 926
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 4 cut(s) 696, 1239, 1311, 1351
Alw44I GTGCAC 1 cut(s) 245
AlwI GGATC 5 cut(s) 455, 849, 969, 982, 1071
AoxI GGCC 4 cut(s) 646, 898, 1328, 1364
ApaLI GTGCAC 1 cut(s) 245
ApeKI GCWGC 1 cut(s) 823
ApoI RAATTY 3 cut(s) 1020, 1154, 1318
AseI ATTAAT 2 cut(s) 219, 285
Asp700I GAANNNNTTC 1 cut(s) 534
AspS9I GGNCC 5 cut(s) 4, 49, 86, 414, 1329
AsuHPI GGTGA 2 cut(s) 467, 515
AvaII GGWCC 4 cut(s) 4, 49, 86, 414
BaeGI GKGCMC 1 cut(s) 249
BamHI GGATCC 1 cut(s) 974
BanI GGYRCC 2 cut(s) 621, 678
BanII GRGCYC 1 cut(s) 271
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 810
BccI CCATC 3 cut(s) 455, 909, 965
BceAI ACGGC 3 cut(s) 300, 913, 933
BciT130I CCWGG 2 cut(s) 1013, 1215
BclI TGATCA 3 cut(s) 400, 772, 1111
BcoDI GTCTC 4 cut(s) 696, 1239, 1311, 1351
BcuI ACTAGT 1 cut(s) 37
BfaI CTAG 6 cut(s) 38, 506, 698, 741, 923, 966
BfrI CTTAAG 1 cut(s) 544
BglII AGATCT 1 cut(s) 887
BisI GCNGC 3 cut(s) 164, 197, 824
BlsI GCNGC 3 cut(s) 165, 198, 825
Bme1390I CCNGG 2 cut(s) 1013, 1215
Bme18I GGWCC 4 cut(s) 4, 49, 86, 414
BmgT120I GGNCC 5 cut(s) 4, 49, 86, 414, 1329
BmrFI CCNGG 2 cut(s) 1013, 1215
BmsI GCATC 4 cut(s) 160, 727, 940, 1084
BpmI CTGGAG 1 cut(s) 1052
BpuEI CTTGAG 1 cut(s) 886
Bsa29I ATCGAT 2 cut(s) 186, 1289
BsaI GGTCTC 2 cut(s) 1239, 1351
BsaJI CCNNGG 9 cut(s) 417, 649, 837, 895, 1011, 1174, 1325, 1361, 1381
Bsc4I CCNNNNNNNGG 6 cut(s) 339, 340, 423, 532, 1220, 1382
Bse118I RCCGGY 1 cut(s) 644
Bse1I ACTGG 6 cut(s) 130, 680, 824, 1069, 1282, 1394
Bse3DI GCAATG 2 cut(s) 180, 1114
BseBI CCWGG 2 cut(s) 1013, 1215
BseCI ATCGAT 2 cut(s) 186, 1289
BseDI CCNNGG 9 cut(s) 417, 649, 837, 895, 1011, 1174, 1325, 1361, 1381
BseGI GGATG 5 cut(s) 592, 742, 931, 976, 1075
BseLI CCNNNNNNNGG 6 cut(s) 339, 340, 423, 532, 1220, 1382
BseMI GCAATG 2 cut(s) 180, 1114
BseNI ACTGG 6 cut(s) 130, 680, 824, 1069, 1282, 1394
BseRI GAGGAG 1 cut(s) 81
BseSI GKGCMC 1 cut(s) 249
BseXI GCAGC 1 cut(s) 810
BseYI CCCAGC 1 cut(s) 52
BsgI GTGCAG 1 cut(s) 1153
BshFI GGCC 4 cut(s) 648, 900, 1330, 1366
BshNI GGYRCC 2 cut(s) 621, 678
BshVI ATCGAT 2 cut(s) 186, 1289
BsiHKAI GWGCWC 1 cut(s) 249
BsiSI CCGG 1 cut(s) 645
BslFI GGGAC 3 cut(s) 35, 554, 976
BslI CCNNNNNNNGG 6 cut(s) 339, 340, 423, 532, 1220, 1382
BsmAI GTCTC 4 cut(s) 696, 1239, 1311, 1351
BsmBI CGTCTC 1 cut(s) 1311
BsmFI GGGAC 3 cut(s) 35, 554, 976
BsmI GAATGC 1 cut(s) 790
BsnI GGCC 4 cut(s) 648, 900, 1330, 1366
Bso31I GGTCTC 2 cut(s) 1239, 1351
Bsp1286I GDGCHC 2 cut(s) 249, 271
Bsp1407I TGTACA 1 cut(s) 254
Bsp19I CCATGG 2 cut(s) 417, 649
BspACI CCGC 5 cut(s) 22, 67, 84, 163, 197
BspANI GGCC 4 cut(s) 648, 900, 1330, 1366
BspDI ATCGAT 2 cut(s) 186, 1289
BspHI TCATGA 1 cut(s) 397
BspPI GGATC 5 cut(s) 455, 849, 969, 982, 1071
BspT107I GGYRCC 2 cut(s) 621, 678
BspTI CTTAAG 1 cut(s) 544
BspTNI GGTCTC 2 cut(s) 1239, 1351
BsrDI GCAATG 2 cut(s) 180, 1114
BsrFI RCCGGY 1 cut(s) 644
BsrGI TGTACA 1 cut(s) 254
BsrI ACTGG 6 cut(s) 130, 680, 824, 1069, 1282, 1394
BssAI RCCGGY 1 cut(s) 644
BssECI CCNNGG 9 cut(s) 417, 649, 837, 895, 1011, 1174, 1325, 1361, 1381
BssT1I CCWWGG 6 cut(s) 417, 649, 837, 1174, 1325, 1361
Bst2UI CCWGG 2 cut(s) 1013, 1215
Bst4CI ACNGT 8 cut(s) 240, 598, 621, 719, 732, 1037, 1043, 1305
Bst6I CTCTTC 3 cut(s) 214, 479, 1237
BstAFI CTTAAG 1 cut(s) 544
BstAUI TGTACA 1 cut(s) 254
BstDEI CTNAG 1 cut(s) 884
BstDSI CCRYGG 4 cut(s) 417, 649, 895, 1381
BstF5I GGATG 5 cut(s) 592, 742, 931, 976, 1075
BstMAI GTCTC 4 cut(s) 696, 1239, 1311, 1351
BstMWI GCNNNNNNNGC 2 cut(s) 997, 1235
BstNI CCWGG 2 cut(s) 1013, 1215
BstSCI CCNGG 2 cut(s) 1011, 1213
BstSLI GKGCMC 1 cut(s) 249
BstV1I GCAGC 1 cut(s) 810
BstX2I RGATCY 4 cut(s) 447, 887, 974, 1063
BstYI RGATCY 4 cut(s) 447, 887, 974, 1063
Bsu15I ATCGAT 2 cut(s) 186, 1289
BsuRI GGCC 4 cut(s) 648, 900, 1330, 1366
BsuTUI ATCGAT 2 cut(s) 186, 1289
BtgI CCRYGG 4 cut(s) 417, 649, 895, 1381
BtsCI GGATG 5 cut(s) 592, 742, 931, 976, 1075
BtsIMutI CAGTG 4 cut(s) 123, 724, 964, 1076
CciI TCATGA 1 cut(s) 397
Cfr10I RCCGGY 1 cut(s) 644
Cfr13I GGNCC 5 cut(s) 4, 49, 86, 414, 1329
ClaI ATCGAT 2 cut(s) 186, 1289
CsiI ACCWGGT 1 cut(s) 1213
Csp6I GTAC 1 cut(s) 255
CviAII CATG 7 cut(s) 377, 398, 418, 493, 579, 650, 1001
CviQI GTAC 1 cut(s) 255
DdeI CTNAG 1 cut(s) 884
DrdI GACNNNNNNGTC 1 cut(s) 722
DseDI GACNNNNNNGTC 1 cut(s) 722
EaeI YGGCCR 2 cut(s) 646, 898
Eam1104I CTCTTC 3 cut(s) 214, 479, 1237
EarI CTCTTC 3 cut(s) 214, 479, 1237
Eco130I CCWWGG 6 cut(s) 417, 649, 837, 1174, 1325, 1361
Eco24I GRGCYC 1 cut(s) 271
Eco31I GGTCTC 2 cut(s) 1239, 1351
Eco47I GGWCC 4 cut(s) 4, 49, 86, 414
Eco57I CTGAAG 2 cut(s) 687, 963
EcoO109I RGGNCCY 1 cut(s) 1329
EcoRII CCWGG 2 cut(s) 1011, 1213
EcoT14I CCWWGG 6 cut(s) 417, 649, 837, 1174, 1325, 1361
EcoT38I GRGCYC 1 cut(s) 271
ErhI CCWWGG 6 cut(s) 417, 649, 837, 1174, 1325, 1361
Esp3I CGTCTC 1 cut(s) 1311
FaeI CATG 7 cut(s) 380, 401, 421, 496, 582, 653, 1004
FaqI GGGAC 3 cut(s) 35, 554, 976
FatI CATG 7 cut(s) 376, 397, 417, 492, 578, 649, 1000
FauNDI CATATG 2 cut(s) 1089, 1336
FbaI TGATCA 3 cut(s) 400, 772, 1111
Fnu4HI GCNGC 3 cut(s) 164, 197, 824
FokI GGATG 5 cut(s) 599, 749, 918, 983, 1062
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 3 cut(s) 164, 197, 824
FspBI CTAG 6 cut(s) 38, 506, 698, 741, 923, 966
GluI GCNGC 3 cut(s) 164, 197, 824
GsaI CCCAGC 1 cut(s) 56
GsuI CTGGAG 1 cut(s) 1052
HaeIII GGCC 4 cut(s) 648, 900, 1330, 1366
HapII CCGG 1 cut(s) 645
Hin1II CATG 7 cut(s) 380, 401, 421, 496, 582, 653, 1004
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HindIII AAGCTT 2 cut(s) 547, 854
HinfI GANTC 6 cut(s) 388, 515, 530, 1165, 1286, 1297
HpaII CCGG 1 cut(s) 645
HphI GGTGA 2 cut(s) 467, 515
Hpy166II GTNNAC 3 cut(s) 247, 348, 673
Hpy188I TCNGA 6 cut(s) 107, 140, 558, 586, 906, 1164
Hpy188III TCNNGA 5 cut(s) 398, 539, 664, 1140, 1191
Hpy8I GTNNAC 3 cut(s) 247, 348, 673
HpyAV CCTTC 4 cut(s) 41, 102, 315, 871
HpyCH4III ACNGT 8 cut(s) 240, 598, 621, 719, 732, 1037, 1043, 1305
HpyCH4V TGCA 6 cut(s) 173, 247, 790, 985, 1031, 1134
HpyF10VI GCNNNNNNNGC 2 cut(s) 997, 1235
HpyF3I CTNAG 1 cut(s) 884
Hsp92II CATG 7 cut(s) 380, 401, 421, 496, 582, 653, 1004
Ksp22I TGATCA 3 cut(s) 400, 772, 1111
LmnI GCTCC 2 cut(s) 312, 335
Lsp1109I GCAGC 1 cut(s) 810
LweI GCATC 4 cut(s) 160, 727, 940, 1084
MabI ACCWGGT 1 cut(s) 1213
MaeI CTAG 6 cut(s) 38, 506, 698, 741, 923, 966
MaeIII GTNAC 6 cut(s) 488, 815, 913, 1037, 1043, 1346
MboII GAAGA 7 cut(s) 201, 204, 270, 452, 496, 956, 1254
MflI RGATCY 4 cut(s) 447, 887, 974, 1063
MhlI GDGCHC 2 cut(s) 249, 271
MluCI AATT 9 cut(s) 259, 380, 562, 781, 1020, 1154, 1224, 1256, 1318
MmeI TCCRAC 4 cut(s) 536, 635, 1180, 1201
MnlI CCTC 9 cut(s) 36, 99, 102, 217, 378, 458, 861, 945, 1187
MroXI GAANNNNTTC 1 cut(s) 534
MseI TTAA 7 cut(s) 219, 285, 372, 545, 600, 798, 852
MspCI CTTAAG 1 cut(s) 544
MspI CCGG 1 cut(s) 645
MspR9I CCNGG 2 cut(s) 1013, 1215
Mva1269I GAATGC 1 cut(s) 790
MvaI CCWGG 2 cut(s) 1013, 1215
MwoI GCNNNNNNNGC 2 cut(s) 997, 1235
NcoI CCATGG 2 cut(s) 417, 649
NdeI CATATG 2 cut(s) 1089, 1336
NlaIII CATG 7 cut(s) 380, 401, 421, 496, 582, 653, 1004
NmuCI GTSAC 1 cut(s) 913
PagI TCATGA 1 cut(s) 397
PctI GAATGC 1 cut(s) 790
PdmI GAANNNNTTC 1 cut(s) 534
PfeI GAWTC 6 cut(s) 388, 515, 530, 1165, 1286, 1297
PflMI CCANNNNNTGG 2 cut(s) 423, 1220
PkrI GCNGC 3 cut(s) 165, 198, 825
PshBI ATTAAT 2 cut(s) 219, 285
Psp6I CCWGG 2 cut(s) 1011, 1213
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 2 cut(s) 1011, 1213
PspPI GGNCC 5 cut(s) 4, 49, 86, 414, 1329
PsuI RGATCY 4 cut(s) 447, 887, 974, 1063
RsaI GTAC 1 cut(s) 256
RsaNI GTAC 1 cut(s) 255
SaqAI TTAA 7 cut(s) 219, 285, 372, 545, 600, 798, 852
SatI GCNGC 3 cut(s) 164, 197, 824
Sau96I GGNCC 5 cut(s) 4, 49, 86, 414, 1329
ScrFI CCNGG 2 cut(s) 1013, 1215
SduI GDGCHC 2 cut(s) 249, 271
SexAI ACCWGGT 1 cut(s) 1213
SfaNI GCATC 4 cut(s) 160, 727, 940, 1084
SinI GGWCC 4 cut(s) 4, 49, 86, 414
SmlI CTYRAG 2 cut(s) 544, 865
SmoI CTYRAG 2 cut(s) 544, 865
SpeI ACTAGT 1 cut(s) 37
Sse9I AATT 9 cut(s) 259, 380, 562, 781, 1020, 1154, 1224, 1256, 1318
SsiI CCGC 5 cut(s) 22, 67, 84, 163, 197
SspI AATATT 1 cut(s) 950
SspMI CTAG 6 cut(s) 38, 506, 698, 741, 923, 966
StyD4I CCNGG 2 cut(s) 1011, 1213
StyI CCWWGG 6 cut(s) 417, 649, 837, 1174, 1325, 1361
TaaI ACNGT 8 cut(s) 240, 598, 621, 719, 732, 1037, 1043, 1305
TaqI TCGA 5 cut(s) 186, 215, 281, 289, 1289
TasI AATT 9 cut(s) 259, 380, 562, 781, 1020, 1154, 1224, 1256, 1318
TatI WGTACW 1 cut(s) 254
TauI GCSGC 2 cut(s) 166, 199
TfiI GAWTC 6 cut(s) 388, 515, 530, 1165, 1286, 1297
Tru1I TTAA 7 cut(s) 219, 285, 372, 545, 600, 798, 852
Tru9I TTAA 7 cut(s) 219, 285, 372, 545, 600, 798, 852
TscAI CASTG 4 cut(s) 130, 724, 964, 1076
TseFI GTSAC 1 cut(s) 913
TseI GCWGC 1 cut(s) 823
Tsp45I GTSAC 1 cut(s) 913
TspDTI ATGAA 8 cut(s) 393, 453, 509, 528, 567, 753, 890, 1017
TspGWI ACGGA 1 cut(s) 1398
TspRI CASTG 4 cut(s) 130, 724, 964, 1076
Van91I CCANNNNNTGG 2 cut(s) 423, 1220
Vha464I CTTAAG 1 cut(s) 544
VneI GTGCAC 1 cut(s) 245
VpaK11BI GGWCC 4 cut(s) 4, 49, 86, 414
VspI ATTAAT 2 cut(s) 219, 285
XapI RAATTY 3 cut(s) 1020, 1154, 1318
XcmI CCANNNNNNNNNTGG 1 cut(s) 1181
XmnI GAANNNNTTC 1 cut(s) 534
XspI CTAG 6 cut(s) 38, 506, 698, 741, 923, 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.