Rh1BG048200

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000356
Physical Location & Seq
Forward (+)
6622046 .. 6633108
11063 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG048200.1

Sequence Viewer

Length: 2217 bp
ATGATGTACCCTGATCCTCAACAGCAGCAGCAACACCAACAGCAGCAGCAGCAGCAGCAACAACAACACTATCACCAGCAGCAGCAGCAACACCACCAGCACCAGCAGCAACACCATCAGCATCAGCAGCAGCAGCAGCAGCAGCAAGGTGGAGACTTTCACAGGGGCCCACCGCCGATGATGCGTCAGCCGTCTGCTTCTTCTACTAACATGGCTCCGGATTATCACCACCCCTCCGCCCCTGGTCCTCCTGTTCCTCCTTATGATGTTCATGGTGATGGTTTTGGTGCAAAGAGGAACAGAAAGCAGACTCAGAGAAGGGCAGTTGATTACACTAGCACTGTCGTGCGGTATACACAGACTCGAATGTGGCAGCGGGATGCGAGGGATAGGACAGTATTGCAACCTACACCAGCAGCTGCAATTGACATGTTGCCAACAGTTGCCTATTCAAATAACCCATCTACGGGCTTTGCTGCAAAGTTTGTGCATACTTCCACAAACAAAGTCCGTAATTCAATTAATCGGGTTTTGTGGACTCCTCCTGGGAGGCGTCTTATTACAGGCTCTCAAAATGGGGAATTCACTCTTTGGAATGGCCAATCATTTAATTTTGAAATGATTCTTCAGGCTCATGATCAAGCAATCAGATCTATGGTCTGGAGTCATAATGAAAATTGGATGGTCTCTGGTGATGATGGAGGATCAATAAAGTATTGGCAGACCAACATGAATAATGTGAAGGCCAATAAATCTGCTCACAAAGAATCTGTTCAGGACTTAAGCTTCTGTAAGACTGACTTGAAGTTCTGTTCCTGTTCGAATGATACATCTGTTAAAATATGGGATTTTGCACGGTGCCAAGAAGAGCGCACATTGACTGGCCATGGTTGGGATGTGAAGAGTGTTGACTGGCACCCTACAAAGTCTCTACTAGCTTCAGGTGGGAAAGACCAGCTTGTCAAACTTTGGGATGCTAAGTCAGGGCGAGAACTATGTTCATTTCATGGTCACAAAAATTTGGTGCAATCTGTTAAATGGAACCAAAATGGCAACTGGCTGCTAACTGCTTCTAAGGATCAAGTCATAAAGCTTTACGACATAAGGGCTATGAAGGAGCTTGAATCTTTCCGTGGGCATCGGAAGGAAGTGACTGCTCTGGCTTGGCATCCTTTTCATGAAGAATATTTTGTCAGTGGGAGTTCTGATGGATCCATTTTCCATTGGCTTGTTGGGCATGAAACTCCCCAGGTTGAAATTGCTAATGCACACGATAACAGTGTGTGGGATCTCCAATGGCACCCTATTGGCTATATTCTTTGCAGTGGTAGCAATGATCACACAACAAAGTTTTGGTGCAGAAACAGGCCAGGAGACACTGGTCGCGATAAATTTAACATCAATCAGACTCAAGGTTATGGTGATCAAAACTCTGCTTTTGCTGGTCGCATGACGGGTAATTTTCCATTACACGATGGGCCACCAACTCCAGGACCATTTACTCCCGGGGGAACTCGAAATGAAGGAACCATTCCAGGGGTTGGAGTAGCTATGCCGTTAGCCATACCATCCCTTGACACATCTATTCAGGGAGAACAACAGCAACCTCATGCACATTCAATGCCTTTAGGAGCTCCTCCTCTCCCACCTGGTCCACATCCTTCTCTTCTTGCTGCAAATCAGCAGCAACCATATCATCAAAATGCGCAACAGCAACATCAGCAACACCAAGGTCACCCACAACAAATGCCCCCTTTGCCTATGCCACCTCCAAATATGCCACAGCTACAGCCTCCATCCCATTTACCCTTGCTGCCGCATCCTCATTTACGTCCACCACCCCAAATGCCCCCACTCGGTATGCCATCTTCAGTGTCCGGATCTTTGCCTATGCCTTCATCAGTTCCAACATCACTTTCAATGCCAATGGGCATGCAAGGTACAATGAATCAGATGGTTCATCAATTGCCACAAGGTCATTATATGGGCATGAACCCACCTCCACCTGCTGGAGGTTTCCCAAATGGGATGCCGAACATGCAGGGCCCATCTAATGCAAGTGGAGGGCAAATGTATTCTCATGCTGGCACCTTCAATCGTGCACAATCTGGACAAATGCCAATGCTACCGGGGTATAATCCCTACCAGCCCAGGGGTCAATCCGGTATGCCTCAGCCTCCACCAGGGCCACCACCACATGGGCAAACACCTCAATAG

Protein Analysis

738

Amino Acids

82.39

Weight (kDa)

8.89

Isoelectric Point (pI)

54.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_MABP1-WDR62_2nd PF24782 122 - 409 2.7e-14 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 150 - 335 1.2e-25 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 164 - 242 2.7e-06 WDR3 second beta-propeller domain
WDR55 PF24796 166 - 244 6.8e-06 WDR55
Beta-prop_WDR3_1st PF25173 167 - 319 6.6e-23 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 169 - 342 9.4e-27 THOC3 beta-propeller domain
Beta-prop_IFT140_1st PF23383 173 - 259 2.6e-09 IFT140 first beta-propeller
WD40_Gbeta PF25391 174 - 377 4.6e-20 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 182 - 248 2.5e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 183 - 376 2.4e-31 Prp19 WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 204 - 320 1.4e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 207 - 240 1e-05 WD domain, G-beta repeat
EIF3I PF24805 208 - 290 1.8e-08 EIF3I
Beta-prop_TEP1_2nd PF25047 217 - 387 1.3e-14 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 229 - 335 1.1e-09 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 236 - 325 8.8e-10 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 236 - 351 2.2e-23 WDR5 beta-propeller domain
WDR55 PF24796 245 - 409 3.1e-16 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 249 - 410 1.8e-18 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 265 - 432 3.1e-20 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 269 - 357 5.6e-06 WDR90/POC16, second beta-propeller
Beta-prop_IFT140_1st PF23383 279 - 364 3.9e-06 IFT140 first beta-propeller
Beta-prop_WDR3_1st PF25173 288 - 409 2.4e-27 WDR3 first beta-propeller domain
WD40 PF00400 289 - 325 4.3e-09 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 291 - 372 1.4e-11 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 292 - 451 9.3e-14 EIF3I
Beta-prop_WDR3_2nd PF25172 292 - 412 6.8e-14 WDR3 second beta-propeller domain
Beta-prop_EML PF23409 293 - 399 4e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin PF25460 299 - 408 1.5e-06 Aladin seven-bladed propeller
Beta-prop_WDR19_1st PF23389 303 - 450 9.3e-08 WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 320 - 453 1.4e-10 WDR36/Utp21 first beta-propeller
WD40 PF00400 330 - 367 5.8e-08 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 331 - 454 4.7e-22 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 336 - 453 1.4e-20 WDR5 beta-propeller domain
ANAPC4_WD40 PF12894 337 - 390 8.7e-06 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_THOC3 PF25174 338 - 455 2e-22 THOC3 beta-propeller domain
Beta-prop_EIPR1 PF23609 338 - 406 2.1e-06 EIPR1 beta-propeller
WD40_WDHD1_1st PF24817 342 - 419 2.3e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 374 - 452 3.5e-10 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica Prupe.2G034300_v2.0.a1
pyrus_communis pycom02g24420 pycom07g02650
rosa_chinensis RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2014
AasI GACNNNNNNGTC 1 cut(s) 959
Acc16I TGCGCA 1 cut(s) 1707
Acc36I ACCTGC 1 cut(s) 2014
AccB1I GGYRCC 4 cut(s) 858, 915, 1299, 2087
AccB7I CCANNNNNTGG 3 cut(s) 1541, 2009, 2198
AccI GTMKAC 1 cut(s) 353
AccII CGCG 1 cut(s) 1386
AccIII TCCGGA 2 cut(s) 217, 1877
AciI CCGC 5 cut(s) 173, 237, 349, 376, 1817
AclWI GGATC 7 cut(s) 8, 712, 1086, 1206, 1219, 1296, 1888
AcoI YGGCCR 2 cut(s) 598, 883
AcsI RAATTY 3 cut(s) 581, 1018, 1391
AcuI CTGAAG 3 cut(s) 611, 924, 1854
AcyI GRCGYC 1 cut(s) 553
AfaI GTAC 2 cut(s) 8, 1942
AflII CTTAAG 1 cut(s) 781
AflIII ACRYGT 1 cut(s) 429
AgsI TTSAA 9 cut(s) 453, 519, 617, 805, 1124, 1256, 1620, 1920, 2095
AjnI CCWGG 9 cut(s) 241, 544, 1248, 1369, 1489, 1534, 1648, 2150, 2182
AjuI GAANNNNNNNTTGG 2 cut(s) 1900, 1932
AleI CACNNNNGTG 1 cut(s) 344
AluBI AGCT 9 cut(s) 419, 786, 938, 958, 1093, 1120, 1550, 1634, 1786
AluI AGCT 9 cut(s) 419, 786, 938, 958, 1093, 1120, 1550, 1634, 1786
Alw21I GWGCWC 2 cut(s) 1636, 2104
Alw26I GTCTC 4 cut(s) 147, 691, 933, 1368
Alw44I GTGCAC 1 cut(s) 2100
AlwI GGATC 7 cut(s) 8, 712, 1086, 1206, 1219, 1296, 1888
AlwNI CAGNNNCTG 1 cut(s) 419
Ama87I CYCGRG 1 cut(s) 1505
Aor13HI TCCGGA 2 cut(s) 217, 1877
AoxI GGCC 8 cut(s) 166, 598, 744, 883, 1367, 1478, 2044, 2186
ApaI GGGCCC 2 cut(s) 170, 2048
ApaLI GTGCAC 1 cut(s) 2100
ApoI RAATTY 3 cut(s) 581, 1018, 1391
AseI ATTAAT 1 cut(s) 522
Asp700I GAANNNNTTC 2 cut(s) 621, 771
AspLEI GCGC 2 cut(s) 873, 1708
AspS9I GGNCC 9 cut(s) 166, 167, 245, 1478, 1493, 1652, 2044, 2045, 2186
AsuC2I CCSGG 3 cut(s) 1506, 1507, 2130
AsuHPI GGTGA 6 cut(s) 65, 218, 287, 704, 1433, 1727
AsuII TTCGAA 1 cut(s) 821
AvaI CYCGRG 1 cut(s) 1505
AvaII GGWCC 3 cut(s) 245, 1493, 1652
BaeGI GKGCMC 3 cut(s) 170, 2048, 2104
BaeI ACNNNNGTAYC 2 cut(s) 819, 852
BalI TGGCCA 2 cut(s) 600, 885
BamHI GGATCC 1 cut(s) 1211
BanI GGYRCC 4 cut(s) 858, 915, 1299, 2087
BanII GRGCYC 3 cut(s) 170, 1636, 2048
BarI GAAGNNNNNNTAC 2 cut(s) 1852, 1884
Bbv12I GWGCWC 2 cut(s) 1636, 2104
BbvCI CCTCAGC 1 cut(s) 2172
BceAI ACGGC 2 cut(s) 175, 1540
BciT130I CCWGG 9 cut(s) 243, 546, 1250, 1371, 1491, 1536, 1650, 2152, 2184
BclI TGATCA 3 cut(s) 637, 1336, 1423
BcnI CCSGG 3 cut(s) 1506, 1507, 2130
BcoDI GTCTC 4 cut(s) 147, 691, 933, 1368
BfaI CTAG 2 cut(s) 336, 935
BfmI CTRYAG 1 cut(s) 1787
BfrI CTTAAG 1 cut(s) 781
BfuAI ACCTGC 1 cut(s) 2014
BglII AGATCT 1 cut(s) 650
Bme18I GGWCC 3 cut(s) 245, 1493, 1652
BmeT110I CYCGRG 1 cut(s) 1505
BmgT120I GGNCC 9 cut(s) 166, 167, 245, 1478, 1493, 1652, 2044, 2045, 2186
BmsI GCATC 8 cut(s) 130, 171, 370, 964, 1147, 1177, 1828, 2019
BoxI GACNNNNGTC 1 cut(s) 1380
BpmI CTGGAG 3 cut(s) 682, 1473, 2031
Bpu10I CCTNAGC 1 cut(s) 2172
Bpu14I TTCGAA 1 cut(s) 821
BpuEI CTTGAG 1 cut(s) 1395
BpuMI CCSGG 3 cut(s) 1506, 1507, 2130
BsaHI GRCGYC 1 cut(s) 553
BsaI GGTCTC 1 cut(s) 691
BsaWI WCCGGW 3 cut(s) 217, 1877, 2162
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bse1I ACTGG 4 cut(s) 886, 917, 1061, 1384
Bse3DI GCAATG 1 cut(s) 1339
BseAI TCCGGA 2 cut(s) 217, 1877
BseBI CCWGG 9 cut(s) 243, 546, 1250, 1371, 1491, 1536, 1650, 2152, 2184
BseMI GCAATG 1 cut(s) 1339
BseMII CTCAG 2 cut(s) 326, 2186
BseNI ACTGG 4 cut(s) 886, 917, 1061, 1384
BseRI GAGGAG 3 cut(s) 531, 1626, 1629
BseSI GKGCMC 3 cut(s) 170, 2048, 2104
BsgI GTGCAG 1 cut(s) 1378
Bsh1236I CGCG 1 cut(s) 1386
BshFI GGCC 8 cut(s) 168, 600, 746, 885, 1369, 1480, 2046, 2188
BshNI GGYRCC 4 cut(s) 858, 915, 1299, 2087
BsiHKAI GWGCWC 2 cut(s) 1636, 2104
BsiHKCI CYCGRG 1 cut(s) 1505
BsiSI CCGG 5 cut(s) 218, 1506, 1878, 2129, 2163
BsmAI GTCTC 4 cut(s) 147, 691, 933, 1368
BsnI GGCC 8 cut(s) 168, 600, 746, 885, 1369, 1480, 2046, 2188
Bso31I GGTCTC 1 cut(s) 691
BsoBI CYCGRG 1 cut(s) 1505
Bsp119I TTCGAA 1 cut(s) 821
Bsp120I GGGCCC 2 cut(s) 166, 2044
Bsp1286I GDGCHC 4 cut(s) 170, 1636, 2048, 2104
Bsp13I TCCGGA 2 cut(s) 217, 1877
Bsp19I CCATGG 1 cut(s) 886
Bsp68I TCGCGA 1 cut(s) 1386
BspACI CCGC 5 cut(s) 173, 237, 349, 376, 1817
BspANI GGCC 8 cut(s) 168, 600, 746, 885, 1369, 1480, 2046, 2188
BspCNI CTCAG 2 cut(s) 325, 2185
BspEI TCCGGA 2 cut(s) 217, 1877
BspFNI CGCG 1 cut(s) 1386
BspHI TCATGA 2 cut(s) 634, 1177
BspMI ACCTGC 1 cut(s) 2014
BspPI GGATC 7 cut(s) 8, 712, 1086, 1206, 1219, 1296, 1888
BspQI GCTCTTC 1 cut(s) 861
BspT104I TTCGAA 1 cut(s) 821
BspT107I GGYRCC 4 cut(s) 858, 915, 1299, 2087
BspTI CTTAAG 1 cut(s) 781
BspTNI GGTCTC 1 cut(s) 691
BsrDI GCAATG 1 cut(s) 1339
BsrI ACTGG 4 cut(s) 886, 917, 1061, 1384
BssNAI GTATAC 1 cut(s) 354
BssNI GRCGYC 1 cut(s) 553
BssT1I CCWWGG 2 cut(s) 886, 1729
Bst1107I GTATAC 1 cut(s) 354
Bst2UI CCWGG 9 cut(s) 243, 546, 1250, 1371, 1491, 1536, 1650, 2152, 2184
Bst4CI ACNGT 5 cut(s) 343, 397, 442, 858, 1280
Bst6I CTCTTC 3 cut(s) 861, 896, 1671
BstACI GRCGYC 1 cut(s) 553
BstAFI CTTAAG 1 cut(s) 781
BstBI TTCGAA 1 cut(s) 821
BstC8I GCNNGC 2 cut(s) 1934, 2086
BstDEI CTNAG 4 cut(s) 312, 978, 1074, 2172
BstDSI CCRYGG 2 cut(s) 886, 1132
BstEII GGTNACC 1 cut(s) 1733
BstENI CCTNNNNNAGG 2 cut(s) 2010, 2181
BstFNI CGCG 1 cut(s) 1386
BstHHI GCGC 2 cut(s) 873, 1708
BstMAI GTCTC 4 cut(s) 147, 691, 933, 1368
BstNI CCWGG 9 cut(s) 243, 546, 1250, 1371, 1491, 1536, 1650, 2152, 2184
BstNSI RCATGY 3 cut(s) 433, 1936, 2041
BstPAI GACNNNNGTC 1 cut(s) 1380
BstPI GGTNACC 1 cut(s) 1733
BstSFI CTRYAG 1 cut(s) 1787
BstSLI GKGCMC 3 cut(s) 170, 2048, 2104
BstUI CGCG 1 cut(s) 1386
BstX2I RGATCY 4 cut(s) 650, 1211, 1288, 1880
BstYI RGATCY 4 cut(s) 650, 1211, 1288, 1880
BstZ17I GTATAC 1 cut(s) 354
BsuRI GGCC 8 cut(s) 168, 600, 746, 885, 1369, 1480, 2046, 2188
BtgI CCRYGG 2 cut(s) 886, 1132
BtsI GCAGTG 1 cut(s) 1330
BtsIMutI CAGTG 6 cut(s) 339, 1201, 1285, 1330, 1377, 1878
BtuMI TCGCGA 1 cut(s) 1386
BveI ACCTGC 1 cut(s) 2014
Cac8I GCNNGC 2 cut(s) 1934, 2086
CaiI CAGNNNCTG 1 cut(s) 419
CciI TCATGA 2 cut(s) 634, 1177
CfoI GCGC 2 cut(s) 873, 1708
Cfr13I GGNCC 9 cut(s) 166, 167, 245, 1478, 1493, 1652, 2044, 2045, 2186
Cfr9I CCCGGG 1 cut(s) 1505
CseI GACGC 2 cut(s) 173, 542
CsiI ACCWGGT 1 cut(s) 1648
Csp6I GTAC 2 cut(s) 7, 1941
CviQI GTAC 2 cut(s) 7, 1941
DdeI CTNAG 4 cut(s) 312, 978, 1074, 2172
DrdI GACNNNNNNGTC 1 cut(s) 959
DseDI GACNNNNNNGTC 1 cut(s) 959
EaeI YGGCCR 2 cut(s) 598, 883
Eam1104I CTCTTC 3 cut(s) 861, 896, 1671
EarI CTCTTC 3 cut(s) 861, 896, 1671
EciI GGCGGA 1 cut(s) 226
Ecl136II GAGCTC 1 cut(s) 1634
Eco130I CCWWGG 2 cut(s) 886, 1729
Eco24I GRGCYC 3 cut(s) 170, 1636, 2048
Eco31I GGTCTC 1 cut(s) 691
Eco47I GGWCC 3 cut(s) 245, 1493, 1652
Eco53kI GAGCTC 1 cut(s) 1634
Eco57I CTGAAG 3 cut(s) 611, 924, 1854
Eco88I CYCGRG 1 cut(s) 1505
Eco91I GGTNACC 1 cut(s) 1733
EcoICRI GAGCTC 1 cut(s) 1634
EcoNI CCTNNNNNAGG 2 cut(s) 2010, 2181
EcoO109I RGGNCCY 2 cut(s) 166, 2044
EcoO65I GGTNACC 1 cut(s) 1733
EcoRI GAATTC 1 cut(s) 581
EcoRII CCWGG 9 cut(s) 241, 544, 1248, 1369, 1489, 1534, 1648, 2150, 2182
EcoT14I CCWWGG 2 cut(s) 886, 1729
EcoT38I GRGCYC 3 cut(s) 170, 1636, 2048
ErhI CCWWGG 2 cut(s) 886, 1729
FalI AAGNNNNNCTT 4 cut(s) 785, 817, 942, 974
FauI CCCGC 1 cut(s) 369
FbaI TGATCA 3 cut(s) 637, 1336, 1423
FblI GTMKAC 1 cut(s) 353
FriOI GRGCYC 3 cut(s) 170, 1636, 2048
FspBI CTAG 2 cut(s) 336, 935
FspI TGCGCA 1 cut(s) 1707
GlaI GCGC 2 cut(s) 872, 1707
GsuI CTGGAG 3 cut(s) 682, 1473, 2031
HaeIII GGCC 8 cut(s) 168, 600, 746, 885, 1369, 1480, 2046, 2188
HapII CCGG 5 cut(s) 218, 1506, 1878, 2129, 2163
HgaI GACGC 2 cut(s) 173, 542
HhaI GCGC 2 cut(s) 873, 1708
Hin1I GRCGYC 1 cut(s) 553
Hin6I GCGC 2 cut(s) 871, 1706
HinP1I GCGC 2 cut(s) 871, 1706
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HindIII AAGCTT 2 cut(s) 784, 1091
HinfI GANTC 9 cut(s) 310, 361, 538, 622, 664, 767, 1124, 1408, 1948
HpaII CCGG 5 cut(s) 218, 1506, 1878, 2129, 2163
HphI GGTGA 6 cut(s) 65, 218, 287, 704, 1433, 1727
Hpy166II GTNNAC 6 cut(s) 354, 537, 910, 1655, 1835, 2102
Hpy188I TCNGA 6 cut(s) 315, 650, 1143, 1207, 1407, 1953
Hpy188III TCNNGA 8 cut(s) 218, 635, 661, 776, 1178, 1385, 1878, 2109
Hpy8I GTNNAC 6 cut(s) 354, 537, 910, 1655, 1835, 2102
HpyAV CCTTC 8 cut(s) 312, 736, 1108, 1138, 1517, 1671, 1905, 2101
HpyCH4III ACNGT 5 cut(s) 343, 397, 442, 858, 1280
HpyCH4IV ACGT 1 cut(s) 1831
HpyF3I CTNAG 4 cut(s) 312, 978, 1074, 2172
HpySE526I ACGT 1 cut(s) 1831
Hsp92I GRCGYC 1 cut(s) 553
HspAI GCGC 2 cut(s) 871, 1706
Kpn2I TCCGGA 2 cut(s) 217, 1877
Ksp22I TGATCA 3 cut(s) 637, 1336, 1423
LguI GCTCTTC 1 cut(s) 861
LmnI GCTCC 4 cut(s) 220, 1117, 1631, 1639
LweI GCATC 8 cut(s) 130, 171, 370, 964, 1147, 1177, 1828, 2019
MabI ACCWGGT 1 cut(s) 1648
MaeI CTAG 2 cut(s) 336, 935
MaeII ACGT 1 cut(s) 1831
MaeIII GTNAC 3 cut(s) 1010, 1150, 1733
MboII GAAGA 7 cut(s) 192, 617, 878, 913, 1193, 1658, 1860
MfeI CAATTG 2 cut(s) 423, 1964
MflI RGATCY 4 cut(s) 650, 1211, 1288, 1880
MhlI GDGCHC 4 cut(s) 170, 1636, 2048, 2104
MlsI TGGCCA 2 cut(s) 600, 885
MluNI TGGCCA 2 cut(s) 600, 885
MlyI GAGTC 5 cut(s) 304, 355, 532, 673, 1402
MmeI TCCRAC 2 cut(s) 1522, 1931
Mox20I TGGCCA 2 cut(s) 600, 885
MroI TCCGGA 2 cut(s) 217, 1877
MroXI GAANNNNTTC 2 cut(s) 621, 771
MscI TGGCCA 2 cut(s) 600, 885
MseI TTAA 6 cut(s) 522, 609, 782, 837, 1035, 1395
MslI CAYNNNNRTG 3 cut(s) 276, 344, 1701
Msp20I TGGCCA 2 cut(s) 600, 885
MspA1I CMGCKG 2 cut(s) 376, 419
MspCI CTTAAG 1 cut(s) 781
MspI CCGG 5 cut(s) 218, 1506, 1878, 2129, 2163
MunI CAATTG 2 cut(s) 423, 1964
MvaI CCWGG 9 cut(s) 243, 546, 1250, 1371, 1491, 1536, 1650, 2152, 2184
MvnI CGCG 1 cut(s) 1386
NciI CCSGG 3 cut(s) 1506, 1507, 2130
NcoI CCATGG 1 cut(s) 886
NmuCI GTSAC 3 cut(s) 1010, 1150, 1733
NruI TCGCGA 1 cut(s) 1386
NsbI TGCGCA 1 cut(s) 1707
NspI RCATGY 3 cut(s) 433, 1936, 2041
NspV TTCGAA 1 cut(s) 821
OliI CACNNNNGTG 1 cut(s) 344
PaeI GCATGC 1 cut(s) 1936
PagI TCATGA 2 cut(s) 634, 1177
PaqCI CACCTGC 1 cut(s) 2014
PasI CCCWGGG 1 cut(s) 2151
PciI ACATGT 1 cut(s) 429
PciSI GCTCTTC 1 cut(s) 861
PdmI GAANNNNTTC 2 cut(s) 621, 771
PfeI GAWTC 4 cut(s) 622, 767, 1124, 1948
PflMI CCANNNNNTGG 3 cut(s) 1541, 2009, 2198
PfoI TCCNGGA 1 cut(s) 1489
PleI GAGTC 5 cut(s) 304, 355, 532, 672, 1402
PpsI GAGTC 5 cut(s) 304, 355, 532, 672, 1402
PscI ACATGT 1 cut(s) 429
PshAI GACNNNNGTC 1 cut(s) 1380
PshBI ATTAAT 1 cut(s) 522
Psp124BI GAGCTC 1 cut(s) 1636
Psp6I CCWGG 9 cut(s) 241, 544, 1248, 1369, 1489, 1534, 1648, 2150, 2182
PspEI GGTNACC 1 cut(s) 1733
PspGI CCWGG 9 cut(s) 241, 544, 1248, 1369, 1489, 1534, 1648, 2150, 2182
PspOMI GGGCCC 2 cut(s) 166, 2044
PspPI GGNCC 9 cut(s) 166, 167, 245, 1478, 1493, 1652, 2044, 2045, 2186
PstNI CAGNNNCTG 1 cut(s) 419
PsuI RGATCY 4 cut(s) 650, 1211, 1288, 1880
PvuII CAGCTG 1 cut(s) 419
RruI TCGCGA 1 cut(s) 1386
RsaI GTAC 2 cut(s) 8, 1942
RsaNI GTAC 2 cut(s) 7, 1941
RseI CAYNNNNRTG 3 cut(s) 276, 344, 1701
SacI GAGCTC 1 cut(s) 1636
SapI GCTCTTC 1 cut(s) 861
SaqAI TTAA 6 cut(s) 522, 609, 782, 837, 1035, 1395
Sau96I GGNCC 9 cut(s) 166, 167, 245, 1478, 1493, 1652, 2044, 2045, 2186
SchI GAGTC 5 cut(s) 304, 355, 532, 673, 1402
SduI GDGCHC 4 cut(s) 170, 1636, 2048, 2104
SexAI ACCWGGT 1 cut(s) 1648
SfaNI GCATC 8 cut(s) 130, 171, 370, 964, 1147, 1177, 1828, 2019
SfcI CTRYAG 1 cut(s) 1787
SfuI TTCGAA 1 cut(s) 821
SinI GGWCC 3 cut(s) 245, 1493, 1652
SmaI CCCGGG 1 cut(s) 1507
SmiMI CAYNNNNRTG 3 cut(s) 276, 344, 1701
SmlI CTYRAG 2 cut(s) 781, 1410
SmoI CTYRAG 2 cut(s) 781, 1410
SphI GCATGC 1 cut(s) 1936
SsiI CCGC 5 cut(s) 173, 237, 349, 376, 1817
SspI AATATT 1 cut(s) 1187
SspMI CTAG 2 cut(s) 336, 935
SstI GAGCTC 1 cut(s) 1636
StyI CCWWGG 2 cut(s) 886, 1729
TaaI ACNGT 5 cut(s) 343, 397, 442, 858, 1280
TaiI ACGT 1 cut(s) 1834
TaqI TCGA 3 cut(s) 364, 821, 1516
TauI GCSGC 1 cut(s) 1819
TfiI GAWTC 4 cut(s) 622, 767, 1124, 1948
Tru1I TTAA 6 cut(s) 522, 609, 782, 837, 1035, 1395
Tru9I TTAA 6 cut(s) 522, 609, 782, 837, 1035, 1395
TscAI CASTG 6 cut(s) 346, 1201, 1285, 1330, 1384, 1878
TseFI GTSAC 3 cut(s) 1010, 1150, 1733
Tsp45I GTSAC 3 cut(s) 1010, 1150, 1733
TspGWI ACGGA 2 cut(s) 500, 1121
TspMI CCCGGG 1 cut(s) 1505
TspRI CASTG 6 cut(s) 346, 1201, 1285, 1330, 1384, 1878
Van91I CCANNNNNTGG 3 cut(s) 1541, 2009, 2198
Vha464I CTTAAG 1 cut(s) 781
VneI GTGCAC 1 cut(s) 2100
VpaK11BI GGWCC 3 cut(s) 245, 1493, 1652
VspI ATTAAT 1 cut(s) 522
XagI CCTNNNNNAGG 2 cut(s) 2010, 2181
XapI RAATTY 3 cut(s) 581, 1018, 1391
XceI RCATGY 3 cut(s) 433, 1936, 2041
XcmI CCANNNNNNNNNTGG 1 cut(s) 1229
XmaI CCCGGG 1 cut(s) 1505
XmiI GTMKAC 1 cut(s) 353
XmnI GAANNNNTTC 2 cut(s) 621, 771
XspI CTAG 2 cut(s) 336, 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.