Rw1G004890

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000356
Physical Location & Seq
Forward (+)
9320445 .. 9330686
10242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004890.1

Sequence Viewer

Length: 2208 bp
ATGATGTACCCTGATCCTCAACAGCAGCAGCAACACCAACAGCAGCAGCAGCAGCAACAACAACACTATCACCAGCAGCAGCAGCAACACCACCAGCACCAGCAGCAACACCACCAGCATCAGCAGCAGCAGCAGCAAGGTGGAGACTTTCACAGGGGCCCACCGCCGATGATGCGTCAGCCGTCTGCTTCTTCTACTAACATGGCTCCGGATTATCACCACCCTTCCGCCCCTGGTCCTCCTGTTCCTCCTTATGATGTTCATGGTGATGGTTTTGGTGCAAAGAGGAACAGAAAGCAGACTCAGAGAAGGGCAGTTGATTACACTAGCACTGTCGTGCGGTATACACAGACTAGAATGTGGCAGCGGGATGCGAGGGATAGGACAGTATTGCAACCTACACCAGCAGCAGCAATTGACATGTTGCCGACAGTTGCCTATTCAAATAACCCATCTACGGGCTTTGCTGCAAAGTTTGTGCATACTTCCACAAACAAAGTCCGTAATTCAATTAATCGGGTTTTGTGGACTCCTCCTGGGAGGCGTCTTATTACAGGCTCTCAAAATGGGGAATTCACTCTTTGGAATGGCCAATCATTTAATTTTGAAATGATTCTTCAGGCTCATGATCAAGCAATCAGATCTATGGTCTGGAGTCATAATGAAAATTGGATGGTCTCTGGTGATGATGGAGGATCAATAAAGTATTGGCAGACCAACATGAATAATGTGAAGGCCAATAAATCTGCTCACAAAGAATCTGTTCAGGACTTAAGCTTCTGTAAGACTGACTTGAAGTTCTGTTCCTGTTCGAATGATACTTCTGTTAAAATATGGGATTTTGCACGGTGCCAAGAAGAGCGCACATTGACTGGCCATGGTTGGGATGTGAAGAGTGTTGACTGGCACCCTACAAAGTCTCTACTAGCTTCAGGTGGGAAAGACCAGCTTGTCAAACTTTGGGATGCTAAGTCAGGGCGAGAACTATGTTCATTTCATGGTCACAAAAATTTGGTGCAATCTGTTAAATGGAACCAAAATGGCAACTGGCTGCTAACTGCTTCTAAGGATCAAGTCATAAAGCTTTACGACATAAGGGCTATGAAGGAGCTTGAATCTTTCCGTGGGCATCGGAAGGAAGTGACTGCTCTGGCTTGGCATCCTTTTCATGAAGAATATTTTGTCAGTGGGAGTTCTGATGGATCCATTTTCCATTGGCTTGTTGGTCATGAGACTCCCCAGGTTGAAATTGTTAATGCACACGATAACAGTGTGTGGGATCTCCAATGGCACCCTATTGGCTATATTCTTTGCAGTGGTAGCAATGATCACACAACAAAGTTTTGGTGCAGAAACAGGCCAGGAGACACTGGTCGCGATAAATTTAACATCAATCAGAGTCAAGGTTATGGTGATCAAAACTCTGCTTTTGCTGGTCGCATGACGGGTAATTTTCCATTACACGATGGACCACCAACTCCAGGACCATTTACTCCCGGGGGAACTCGAAATGAAGGAAACATTCCAGGGGTTGGAGTAGCTATGCCGTTAGCCATGCCATCCCTTGACGCATCTATTCAGGGAGAACAACAGCAACCTCATGCACATTCAATGCCTTTAGGAGCTCCTCCTCTCCCACCTGGTCCACATCCTTCTCTTCTTGCTGCAAATCAGCAGCAACCATATCATCAAAATGCGCAACAGCAACATCAGCAACACCAAGGTCACCCACAACAAATGCCCCCTTTGCCTATGCCACCTCCAAATATGCCACAGCTACAGCCTCCATCCCATTTACCCTTACTGCCGCATCCTCATTTACGTCCACCACCCCAAATGCCCCCACTCGGTATGCCATCTTCAGTGTCCGGATCTTTGCCTATGCCTTCATCAGTTCCAACATCACTTTCAATGCCAATGGGCATGCAAGGTACAATGAATCAGATGGTTCATCAATTGCCACAAGGTCATTATATGGGCATGAACCCACCTCCACCTGCTGGAGGTTTCCCAAATGGGATGCCGAACATGCAGGGCCCATCTAATGCAAGTGGAGGGCAAATGTATTCTCATGCTGGCACCTTCAATCGTGCACAATCTGGACAAATGCCAATGCTACCGGGGTATAATCCCTACCAGCCCAGGGGTCAATCCGGTATGCCTCAGCCTCCACCAGGGCCACCACCACATGGGCAAACACCTCAATAG

Protein Analysis

735

Amino Acids

82.02

Weight (kDa)

8.89

Isoelectric Point (pI)

56.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_MABP1-WDR62_2nd PF24782 119 - 406 2.7e-14 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 147 - 331 1.3e-25 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 161 - 239 2.7e-06 WDR3 second beta-propeller domain
WDR55 PF24796 163 - 240 7.4e-06 WDR55
Beta-prop_WDR3_1st PF25173 164 - 316 6.5e-23 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 166 - 326 8.6e-26 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 167 - 340 6.5e-27 THOC3 beta-propeller domain
Beta-prop_IFT140_1st PF23383 170 - 256 2.6e-09 IFT140 first beta-propeller
WD40_Gbeta PF25391 171 - 374 4.5e-20 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 175 - 332 3.9e-15 WDHD1 first WD40 domain
Beta-prop_EML PF23409 180 - 246 1.6e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 180 - 373 2.3e-31 Prp19 WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 201 - 317 1.4e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 204 - 237 1e-05 WD domain, G-beta repeat
EIF3I PF24805 205 - 287 1.8e-08 EIF3I
Beta-prop_TEP1_2nd PF25047 214 - 384 1.3e-14 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 233 - 364 1.3e-28 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 233 - 323 6.6e-10 WDR3 second beta-propeller domain
WDR55 PF24796 242 - 405 5.6e-16 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 246 - 407 1.8e-18 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 262 - 406 4.8e-19 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 266 - 354 5.6e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_1st PF25173 278 - 406 7.1e-29 WDR3 first beta-propeller domain
Beta-prop_IFT140_1st PF23383 285 - 360 4.7e-06 IFT140 first beta-propeller
WD40 PF00400 286 - 322 4.3e-09 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 288 - 369 1.4e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 289 - 409 6.2e-14 WDR3 second beta-propeller domain
EIF3I PF24805 289 - 448 6e-14 EIF3I
Beta-prop_EML PF23409 290 - 396 5.3e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin PF25460 296 - 406 1.5e-06 Aladin seven-bladed propeller
Beta-prop_WDR19_1st PF23389 300 - 447 3.6e-08 WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 317 - 450 2.3e-10 WDR36/Utp21 first beta-propeller
WD40 PF00400 327 - 364 5.8e-08 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 328 - 451 3.4e-22 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 333 - 450 6.4e-21 WDR5 beta-propeller domain
ANAPC4_WD40 PF12894 334 - 387 8.6e-06 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_THOC3 PF25174 335 - 451 1.6e-22 THOC3 beta-propeller domain
Beta-prop_EIPR1 PF23609 335 - 403 2.1e-06 EIPR1 beta-propeller
WD40_WDHD1_1st PF24817 339 - 419 2.1e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 371 - 449 3.1e-10 Prp19 WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 372 - 448 9.5e-06 CAF1B/HIR1 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica Prupe.2G034300_v2.0.a1
pyrus_communis pycom02g24420 pycom07g02650
rosa_chinensis RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2005
AasI GACNNNNNNGTC 1 cut(s) 950
Acc16I TGCGCA 1 cut(s) 1698
Acc36I ACCTGC 1 cut(s) 2005
AccB1I GGYRCC 4 cut(s) 849, 906, 1290, 2078
AccB7I CCANNNNNTGG 3 cut(s) 1532, 2000, 2189
AccI GTMKAC 1 cut(s) 344
AccII CGCG 1 cut(s) 1377
AccIII TCCGGA 2 cut(s) 208, 1868
AciI CCGC 5 cut(s) 164, 228, 340, 367, 1808
AclWI GGATC 7 cut(s) 8, 703, 1077, 1197, 1210, 1287, 1879
AcoI YGGCCR 2 cut(s) 589, 874
AcsI RAATTY 3 cut(s) 572, 1009, 1382
AcuI CTGAAG 3 cut(s) 602, 915, 1845
AcyI GRCGYC 1 cut(s) 544
AfaI GTAC 2 cut(s) 8, 1933
AflII CTTAAG 1 cut(s) 772
AflIII ACRYGT 1 cut(s) 420
AgsI TTSAA 9 cut(s) 444, 510, 608, 796, 1115, 1247, 1611, 1911, 2086
AjnI CCWGG 9 cut(s) 232, 535, 1239, 1360, 1480, 1525, 1639, 2141, 2173
AjuI GAANNNNNNNTTGG 2 cut(s) 1891, 1923
AleI CACNNNNGTG 1 cut(s) 335
AluBI AGCT 8 cut(s) 777, 929, 949, 1084, 1111, 1541, 1625, 1777
AluI AGCT 8 cut(s) 777, 929, 949, 1084, 1111, 1541, 1625, 1777
Alw21I GWGCWC 2 cut(s) 1627, 2095
Alw26I GTCTC 5 cut(s) 138, 682, 924, 1226, 1359
Alw44I GTGCAC 1 cut(s) 2091
AlwI GGATC 7 cut(s) 8, 703, 1077, 1197, 1210, 1287, 1879
Ama87I CYCGRG 1 cut(s) 1496
Aor13HI TCCGGA 2 cut(s) 208, 1868
AoxI GGCC 7 cut(s) 157, 589, 735, 874, 1358, 2035, 2177
ApaI GGGCCC 2 cut(s) 161, 2039
ApaLI GTGCAC 1 cut(s) 2091
ApoI RAATTY 3 cut(s) 572, 1009, 1382
AseI ATTAAT 1 cut(s) 513
Asp700I GAANNNNTTC 2 cut(s) 612, 762
AspLEI GCGC 2 cut(s) 864, 1699
AspS9I GGNCC 9 cut(s) 157, 158, 236, 1469, 1484, 1643, 2035, 2036, 2177
AsuC2I CCSGG 3 cut(s) 1497, 1498, 2121
AsuHPI GGTGA 6 cut(s) 62, 209, 278, 695, 1424, 1718
AsuII TTCGAA 1 cut(s) 812
AvaI CYCGRG 1 cut(s) 1496
AvaII GGWCC 4 cut(s) 236, 1469, 1484, 1643
BaeGI GKGCMC 3 cut(s) 161, 2039, 2095
BaeI ACNNNNGTAYC 2 cut(s) 810, 843
BalI TGGCCA 2 cut(s) 591, 876
BamHI GGATCC 1 cut(s) 1202
BanI GGYRCC 4 cut(s) 849, 906, 1290, 2078
BanII GRGCYC 3 cut(s) 161, 1627, 2039
BarI GAAGNNNNNNTAC 2 cut(s) 1843, 1875
Bbv12I GWGCWC 2 cut(s) 1627, 2095
BbvCI CCTCAGC 1 cut(s) 2163
BceAI ACGGC 2 cut(s) 166, 1531
BciT130I CCWGG 9 cut(s) 234, 537, 1241, 1362, 1482, 1527, 1641, 2143, 2175
BclI TGATCA 3 cut(s) 628, 1327, 1414
BcnI CCSGG 3 cut(s) 1497, 1498, 2121
BcoDI GTCTC 5 cut(s) 138, 682, 924, 1226, 1359
BfaI CTAG 3 cut(s) 327, 354, 926
BfmI CTRYAG 1 cut(s) 1778
BfrI CTTAAG 1 cut(s) 772
BfuAI ACCTGC 1 cut(s) 2005
BglII AGATCT 1 cut(s) 641
Bme18I GGWCC 4 cut(s) 236, 1469, 1484, 1643
BmeT110I CYCGRG 1 cut(s) 1496
BmgT120I GGNCC 9 cut(s) 157, 158, 236, 1469, 1484, 1643, 2035, 2036, 2177
BmsI GCATC 9 cut(s) 127, 162, 361, 955, 1138, 1168, 1580, 1819, 2010
BoxI GACNNNNGTC 1 cut(s) 1371
BpmI CTGGAG 3 cut(s) 673, 1464, 2022
Bpu10I CCTNAGC 1 cut(s) 2163
Bpu14I TTCGAA 1 cut(s) 812
BpuMI CCSGG 3 cut(s) 1497, 1498, 2121
BsaHI GRCGYC 1 cut(s) 544
BsaI GGTCTC 1 cut(s) 682
BsaWI WCCGGW 3 cut(s) 208, 1868, 2153
Bse1I ACTGG 4 cut(s) 877, 908, 1052, 1375
Bse3DI GCAATG 1 cut(s) 1330
BseAI TCCGGA 2 cut(s) 208, 1868
BseBI CCWGG 9 cut(s) 234, 537, 1241, 1362, 1482, 1527, 1641, 2143, 2175
BseMI GCAATG 1 cut(s) 1330
BseMII CTCAG 2 cut(s) 317, 2177
BseNI ACTGG 4 cut(s) 877, 908, 1052, 1375
BseRI GAGGAG 3 cut(s) 522, 1617, 1620
BseSI GKGCMC 3 cut(s) 161, 2039, 2095
BsgI GTGCAG 1 cut(s) 1369
Bsh1236I CGCG 1 cut(s) 1377
BshFI GGCC 7 cut(s) 159, 591, 737, 876, 1360, 2037, 2179
BshNI GGYRCC 4 cut(s) 849, 906, 1290, 2078
BsiHKAI GWGCWC 2 cut(s) 1627, 2095
BsiHKCI CYCGRG 1 cut(s) 1496
BsiSI CCGG 5 cut(s) 209, 1497, 1869, 2120, 2154
BsmAI GTCTC 5 cut(s) 138, 682, 924, 1226, 1359
BsnI GGCC 7 cut(s) 159, 591, 737, 876, 1360, 2037, 2179
Bso31I GGTCTC 1 cut(s) 682
BsoBI CYCGRG 1 cut(s) 1496
Bsp119I TTCGAA 1 cut(s) 812
Bsp120I GGGCCC 2 cut(s) 157, 2035
Bsp1286I GDGCHC 4 cut(s) 161, 1627, 2039, 2095
Bsp13I TCCGGA 2 cut(s) 208, 1868
Bsp19I CCATGG 1 cut(s) 877
Bsp68I TCGCGA 1 cut(s) 1377
BspACI CCGC 5 cut(s) 164, 228, 340, 367, 1808
BspANI GGCC 7 cut(s) 159, 591, 737, 876, 1360, 2037, 2179
BspCNI CTCAG 2 cut(s) 316, 2176
BspEI TCCGGA 2 cut(s) 208, 1868
BspFNI CGCG 1 cut(s) 1377
BspHI TCATGA 3 cut(s) 625, 1168, 1228
BspMI ACCTGC 1 cut(s) 2005
BspPI GGATC 7 cut(s) 8, 703, 1077, 1197, 1210, 1287, 1879
BspQI GCTCTTC 1 cut(s) 852
BspT104I TTCGAA 1 cut(s) 812
BspT107I GGYRCC 4 cut(s) 849, 906, 1290, 2078
BspTI CTTAAG 1 cut(s) 772
BspTNI GGTCTC 1 cut(s) 682
BsrDI GCAATG 1 cut(s) 1330
BsrI ACTGG 4 cut(s) 877, 908, 1052, 1375
BssNAI GTATAC 1 cut(s) 345
BssNI GRCGYC 1 cut(s) 544
BssT1I CCWWGG 2 cut(s) 877, 1720
Bst1107I GTATAC 1 cut(s) 345
Bst2UI CCWGG 9 cut(s) 234, 537, 1241, 1362, 1482, 1527, 1641, 2143, 2175
Bst4CI ACNGT 5 cut(s) 334, 388, 433, 849, 1271
Bst6I CTCTTC 3 cut(s) 852, 887, 1662
BstACI GRCGYC 1 cut(s) 544
BstAFI CTTAAG 1 cut(s) 772
BstBI TTCGAA 1 cut(s) 812
BstC8I GCNNGC 2 cut(s) 1925, 2077
BstDEI CTNAG 4 cut(s) 303, 969, 1065, 2163
BstDSI CCRYGG 2 cut(s) 877, 1123
BstEII GGTNACC 1 cut(s) 1724
BstENI CCTNNNNNAGG 2 cut(s) 2001, 2172
BstFNI CGCG 1 cut(s) 1377
BstHHI GCGC 2 cut(s) 864, 1699
BstMAI GTCTC 5 cut(s) 138, 682, 924, 1226, 1359
BstNI CCWGG 9 cut(s) 234, 537, 1241, 1362, 1482, 1527, 1641, 2143, 2175
BstNSI RCATGY 3 cut(s) 424, 1927, 2032
BstPAI GACNNNNGTC 1 cut(s) 1371
BstPI GGTNACC 1 cut(s) 1724
BstSFI CTRYAG 1 cut(s) 1778
BstSLI GKGCMC 3 cut(s) 161, 2039, 2095
BstUI CGCG 1 cut(s) 1377
BstX2I RGATCY 4 cut(s) 641, 1202, 1279, 1871
BstYI RGATCY 4 cut(s) 641, 1202, 1279, 1871
BstZ17I GTATAC 1 cut(s) 345
BsuRI GGCC 7 cut(s) 159, 591, 737, 876, 1360, 2037, 2179
BtgI CCRYGG 2 cut(s) 877, 1123
BtsI GCAGTG 1 cut(s) 1321
BtsIMutI CAGTG 6 cut(s) 330, 1192, 1276, 1321, 1368, 1869
BtuMI TCGCGA 1 cut(s) 1377
BveI ACCTGC 1 cut(s) 2005
Cac8I GCNNGC 2 cut(s) 1925, 2077
CciI TCATGA 3 cut(s) 625, 1168, 1228
CfoI GCGC 2 cut(s) 864, 1699
Cfr13I GGNCC 9 cut(s) 157, 158, 236, 1469, 1484, 1643, 2035, 2036, 2177
Cfr9I CCCGGG 1 cut(s) 1496
CseI GACGC 3 cut(s) 164, 533, 1577
CsiI ACCWGGT 1 cut(s) 1639
Csp6I GTAC 2 cut(s) 7, 1932
CviQI GTAC 2 cut(s) 7, 1932
DdeI CTNAG 4 cut(s) 303, 969, 1065, 2163
DrdI GACNNNNNNGTC 1 cut(s) 950
DseDI GACNNNNNNGTC 1 cut(s) 950
EaeI YGGCCR 2 cut(s) 589, 874
Eam1104I CTCTTC 3 cut(s) 852, 887, 1662
EarI CTCTTC 3 cut(s) 852, 887, 1662
EciI GGCGGA 1 cut(s) 217
Ecl136II GAGCTC 1 cut(s) 1625
Eco130I CCWWGG 2 cut(s) 877, 1720
Eco24I GRGCYC 3 cut(s) 161, 1627, 2039
Eco31I GGTCTC 1 cut(s) 682
Eco47I GGWCC 4 cut(s) 236, 1469, 1484, 1643
Eco53kI GAGCTC 1 cut(s) 1625
Eco57I CTGAAG 3 cut(s) 602, 915, 1845
Eco88I CYCGRG 1 cut(s) 1496
Eco91I GGTNACC 1 cut(s) 1724
EcoICRI GAGCTC 1 cut(s) 1625
EcoNI CCTNNNNNAGG 2 cut(s) 2001, 2172
EcoO109I RGGNCCY 2 cut(s) 157, 2035
EcoO65I GGTNACC 1 cut(s) 1724
EcoRI GAATTC 1 cut(s) 572
EcoRII CCWGG 9 cut(s) 232, 535, 1239, 1360, 1480, 1525, 1639, 2141, 2173
EcoT14I CCWWGG 2 cut(s) 877, 1720
EcoT38I GRGCYC 3 cut(s) 161, 1627, 2039
ErhI CCWWGG 2 cut(s) 877, 1720
FalI AAGNNNNNCTT 4 cut(s) 776, 808, 933, 965
FauI CCCGC 1 cut(s) 360
FbaI TGATCA 3 cut(s) 628, 1327, 1414
FblI GTMKAC 1 cut(s) 344
FriOI GRGCYC 3 cut(s) 161, 1627, 2039
FspBI CTAG 3 cut(s) 327, 354, 926
FspI TGCGCA 1 cut(s) 1698
GlaI GCGC 2 cut(s) 863, 1698
GsuI CTGGAG 3 cut(s) 673, 1464, 2022
HaeIII GGCC 7 cut(s) 159, 591, 737, 876, 1360, 2037, 2179
HapII CCGG 5 cut(s) 209, 1497, 1869, 2120, 2154
HgaI GACGC 3 cut(s) 164, 533, 1577
HhaI GCGC 2 cut(s) 864, 1699
Hin1I GRCGYC 1 cut(s) 544
Hin6I GCGC 2 cut(s) 862, 1697
HinP1I GCGC 2 cut(s) 862, 1697
HincII GTYRAC 1 cut(s) 901
HindII GTYRAC 1 cut(s) 901
HindIII AAGCTT 2 cut(s) 775, 1082
HinfI GANTC 9 cut(s) 301, 529, 613, 655, 758, 1115, 1234, 1399, 1939
HpaII CCGG 5 cut(s) 209, 1497, 1869, 2120, 2154
HphI GGTGA 6 cut(s) 62, 209, 278, 695, 1424, 1718
Hpy166II GTNNAC 6 cut(s) 345, 528, 901, 1646, 1826, 2093
Hpy188I TCNGA 6 cut(s) 306, 641, 1134, 1198, 1398, 1944
Hpy188III TCNNGA 9 cut(s) 209, 626, 652, 767, 1169, 1229, 1376, 1869, 2100
Hpy8I GTNNAC 6 cut(s) 345, 528, 901, 1646, 1826, 2093
HpyAV CCTTC 9 cut(s) 234, 303, 727, 1099, 1129, 1508, 1662, 1896, 2092
HpyCH4III ACNGT 5 cut(s) 334, 388, 433, 849, 1271
HpyCH4IV ACGT 1 cut(s) 1822
HpyF3I CTNAG 4 cut(s) 303, 969, 1065, 2163
HpySE526I ACGT 1 cut(s) 1822
Hsp92I GRCGYC 1 cut(s) 544
HspAI GCGC 2 cut(s) 862, 1697
Kpn2I TCCGGA 2 cut(s) 208, 1868
Ksp22I TGATCA 3 cut(s) 628, 1327, 1414
LguI GCTCTTC 1 cut(s) 852
LmnI GCTCC 4 cut(s) 211, 1108, 1622, 1630
LweI GCATC 9 cut(s) 127, 162, 361, 955, 1138, 1168, 1580, 1819, 2010
MabI ACCWGGT 1 cut(s) 1639
MaeI CTAG 3 cut(s) 327, 354, 926
MaeII ACGT 1 cut(s) 1822
MaeIII GTNAC 3 cut(s) 1001, 1141, 1724
MboII GAAGA 7 cut(s) 183, 608, 869, 904, 1184, 1649, 1851
MfeI CAATTG 2 cut(s) 414, 1955
MflI RGATCY 4 cut(s) 641, 1202, 1279, 1871
MhlI GDGCHC 4 cut(s) 161, 1627, 2039, 2095
MlsI TGGCCA 2 cut(s) 591, 876
MluNI TGGCCA 2 cut(s) 591, 876
MlyI GAGTC 5 cut(s) 295, 523, 664, 1228, 1408
MmeI TCCRAC 2 cut(s) 1513, 1922
Mox20I TGGCCA 2 cut(s) 591, 876
MroI TCCGGA 2 cut(s) 208, 1868
MroXI GAANNNNTTC 2 cut(s) 612, 762
MscI TGGCCA 2 cut(s) 591, 876
MseI TTAA 7 cut(s) 513, 600, 773, 828, 1026, 1254, 1386
MslI CAYNNNNRTG 3 cut(s) 267, 335, 1692
Msp20I TGGCCA 2 cut(s) 591, 876
MspA1I CMGCKG 1 cut(s) 367
MspCI CTTAAG 1 cut(s) 772
MspI CCGG 5 cut(s) 209, 1497, 1869, 2120, 2154
MunI CAATTG 2 cut(s) 414, 1955
MvaI CCWGG 9 cut(s) 234, 537, 1241, 1362, 1482, 1527, 1641, 2143, 2175
MvnI CGCG 1 cut(s) 1377
NciI CCSGG 3 cut(s) 1497, 1498, 2121
NcoI CCATGG 1 cut(s) 877
NmuCI GTSAC 3 cut(s) 1001, 1141, 1724
NruI TCGCGA 1 cut(s) 1377
NsbI TGCGCA 1 cut(s) 1698
NspI RCATGY 3 cut(s) 424, 1927, 2032
NspV TTCGAA 1 cut(s) 812
OliI CACNNNNGTG 1 cut(s) 335
PaeI GCATGC 1 cut(s) 1927
PagI TCATGA 3 cut(s) 625, 1168, 1228
PaqCI CACCTGC 1 cut(s) 2005
PasI CCCWGGG 1 cut(s) 2142
PciI ACATGT 1 cut(s) 420
PciSI GCTCTTC 1 cut(s) 852
PdmI GAANNNNTTC 2 cut(s) 612, 762
PfeI GAWTC 4 cut(s) 613, 758, 1115, 1939
PflMI CCANNNNNTGG 3 cut(s) 1532, 2000, 2189
PfoI TCCNGGA 1 cut(s) 1480
PleI GAGTC 5 cut(s) 295, 523, 663, 1228, 1407
PpsI GAGTC 5 cut(s) 295, 523, 663, 1228, 1407
PscI ACATGT 1 cut(s) 420
PshAI GACNNNNGTC 1 cut(s) 1371
PshBI ATTAAT 1 cut(s) 513
Psp124BI GAGCTC 1 cut(s) 1627
Psp6I CCWGG 9 cut(s) 232, 535, 1239, 1360, 1480, 1525, 1639, 2141, 2173
PspEI GGTNACC 1 cut(s) 1724
PspGI CCWGG 9 cut(s) 232, 535, 1239, 1360, 1480, 1525, 1639, 2141, 2173
PspOMI GGGCCC 2 cut(s) 157, 2035
PspPI GGNCC 9 cut(s) 157, 158, 236, 1469, 1484, 1643, 2035, 2036, 2177
PsuI RGATCY 4 cut(s) 641, 1202, 1279, 1871
RruI TCGCGA 1 cut(s) 1377
RsaI GTAC 2 cut(s) 8, 1933
RsaNI GTAC 2 cut(s) 7, 1932
RseI CAYNNNNRTG 3 cut(s) 267, 335, 1692
SacI GAGCTC 1 cut(s) 1627
SapI GCTCTTC 1 cut(s) 852
SaqAI TTAA 7 cut(s) 513, 600, 773, 828, 1026, 1254, 1386
Sau96I GGNCC 9 cut(s) 157, 158, 236, 1469, 1484, 1643, 2035, 2036, 2177
SchI GAGTC 5 cut(s) 295, 523, 664, 1228, 1408
SduI GDGCHC 4 cut(s) 161, 1627, 2039, 2095
SexAI ACCWGGT 1 cut(s) 1639
SfaNI GCATC 9 cut(s) 127, 162, 361, 955, 1138, 1168, 1580, 1819, 2010
SfcI CTRYAG 1 cut(s) 1778
SfuI TTCGAA 1 cut(s) 812
SinI GGWCC 4 cut(s) 236, 1469, 1484, 1643
SmaI CCCGGG 1 cut(s) 1498
SmiMI CAYNNNNRTG 3 cut(s) 267, 335, 1692
SmlI CTYRAG 1 cut(s) 772
SmoI CTYRAG 1 cut(s) 772
SphI GCATGC 1 cut(s) 1927
SsiI CCGC 5 cut(s) 164, 228, 340, 367, 1808
SspI AATATT 1 cut(s) 1178
SspMI CTAG 3 cut(s) 327, 354, 926
SstI GAGCTC 1 cut(s) 1627
StyI CCWWGG 2 cut(s) 877, 1720
TaaI ACNGT 5 cut(s) 334, 388, 433, 849, 1271
TaiI ACGT 1 cut(s) 1825
TaqI TCGA 2 cut(s) 812, 1507
TauI GCSGC 1 cut(s) 1810
TfiI GAWTC 4 cut(s) 613, 758, 1115, 1939
Tru1I TTAA 7 cut(s) 513, 600, 773, 828, 1026, 1254, 1386
Tru9I TTAA 7 cut(s) 513, 600, 773, 828, 1026, 1254, 1386
TscAI CASTG 6 cut(s) 337, 1192, 1276, 1321, 1375, 1869
TseFI GTSAC 3 cut(s) 1001, 1141, 1724
Tsp45I GTSAC 3 cut(s) 1001, 1141, 1724
TspGWI ACGGA 2 cut(s) 491, 1112
TspMI CCCGGG 1 cut(s) 1496
TspRI CASTG 6 cut(s) 337, 1192, 1276, 1321, 1375, 1869
Van91I CCANNNNNTGG 3 cut(s) 1532, 2000, 2189
Vha464I CTTAAG 1 cut(s) 772
VneI GTGCAC 1 cut(s) 2091
VpaK11BI GGWCC 4 cut(s) 236, 1469, 1484, 1643
VspI ATTAAT 1 cut(s) 513
XagI CCTNNNNNAGG 2 cut(s) 2001, 2172
XapI RAATTY 3 cut(s) 572, 1009, 1382
XceI RCATGY 3 cut(s) 424, 1927, 2032
XcmI CCANNNNNNNNNTGG 1 cut(s) 1220
XmaI CCCGGG 1 cut(s) 1496
XmiI GTMKAC 1 cut(s) 344
XmnI GAANNNNTTC 2 cut(s) 612, 762
XspI CTAG 3 cut(s) 327, 354, 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.