Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0000356

Physical Location & Seq

Genomic Coordinates Forward (+)

27813268 .. 27814404

Gene Structure 1137 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G022590.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 933 bp

ATGGCCCATCACCACCACCCCTACGCCTGTTCTTCGATTAACCATAACCCATTTGCTGCCCAGTTTGTGCGCTCTTGCACAAATTACAGAGCCCGTGCTTCTTCGATCAATCGAGTTTTATGGACACCTTCTGGGAGGCGTCTTATCACAGGCTCTCAAAACGGGGAGTTCACTCTCTGGAATGGTGAGTCATTCAAACATGAATTGAGGATTCAGGGTCATGATCACGCAATCAGGTCCATGGCGTGGAGTTTTGATGATCAAGATTGGATCTCTGGTGATGATGGGGGCACAATCAGGTATTGGAAGAGTAACATGATTAATGTGCTAGTCAATGAATCTGCTCACCATGAATCGGTCAGGGAGAGAACTCTGTTCTTTTATGATCACAAAAATTGGGTGCATTCTGTCAAGTGGAACCGAAATGGTAACTGGCTGCTAACTGCTTCCAAGGATCAAGTCATTAAGCTTTACGACTTGAGGGCTATGAAGGAACTTAGATCTTTCCGCGGCCATCAGAACAAAGTGACTGCTCTGGCTTGGCATCCTCTTACCGAAGATTATTTTGTCAGTGGGAGTAGTGATGGATCCATTTTCCATTGGCTTGTTGGGCATCATACTCCCCAGGTTGAAATTCCTAATGCACACACTAACAGTTACAATAATAATACTGTGTGGGATCTCCAGTGGCATCCTATTGGTCATATGATTTGTAGTGGTAGCAATGATCGCACTACCAAGTTTTGGTGCAGAACAGGAGATAAATCTCAAATCACTCAGAATCAAAGTAATTCTGCTTTTGCTTGTCACATGAACAGTAATTTTCCTTTTCCATGTCACGGACCACCAACTATTGCTACTACTCGAGACGAAGGAACCATTGGTTCAGGAGGTGTTGGAACCATTATACCTGGTGTTGGAATTGCGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

WD40

Protein Analysis

310

Amino Acids

35.22

Weight (kDa)

8.53

Isoelectric Point (pI)

36.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_EML_2	PF23414	25 - 112	2.2e-06	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	27 - 110	1.9e-07	CDC20/Fizzy WD40 domain
Beta-prop_IFT140_1st	PF23383	31 - 119	2.5e-09	IFT140 first beta-propeller
Beta-prop_WDR3_1st	PF25173	31 - 120	2.4e-07	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	32 - 120	7.6e-09	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	34 - 106	1.5e-08	THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	71 - 196	2.6e-08	CAF1B/HIR1 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	77 - 255	1.7e-08	MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz	PF24807	102 - 262	3.3e-19	CDC20/Fizzy WD40 domain
WDR55	PF24796	121 - 269	3.5e-08	WDR55
Beta-prop_WDR3_1st	PF25173	122 - 249	4.5e-17	WDR3 first beta-propeller domain
Beta-prop_DCAF4	PF23761	123 - 187	1.4e-06	DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_EML_2	PF23414	124 - 204	3.3e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd	PF25172	124 - 253	7.5e-11	WDR3 second beta-propeller domain
EIF3I	PF24805	126 - 203	2.4e-06	EIF3I
WD40	PF00400	126 - 159	1.8e-07	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	127 - 212	8.3e-15	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	127 - 201	3.3e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	128 - 179	2.7e-07	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	130 - 262	2.2e-24	THOC3 beta-propeller domain
Beta-prop_Aladin	PF25460	133 - 246	1.4e-06	Aladin seven-bladed propeller
WD40_WDHD1_1st	PF24817	134 - 236	1.8e-12	WDHD1 first WD40 domain
WD40_Prp19	PF24814	134 - 250	4.6e-16	Prp19 WD40 domain
WD40_Gbeta	PF25391	142 - 263	2.4e-07	G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd	PF23393	145 - 247	1e-05	WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	151 - 250	5.4e-06	WDR36/Utp21 first beta-propeller
WD40	PF00400	164 - 201	5.1e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000356)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca	FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica	MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica	Prupe.2G034300_v2.0.a1
pyrus_communis	pycom02g24420 pycom07g02650
rosa_chinensis	RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata	RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora	Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii	Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa	Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha	Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana	Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	246, 744
AccII	CGCG	1 cut(s)	510
AciI	CCGC	3 cut(s)	508, 510, 926
AclWI	GGATC	5 cut(s)	278, 462, 582, 595, 687
AcoI	YGGCCR	1 cut(s)	511
AcsI	RAATTY	1 cut(s)	633
AcyI	GRCGYC	1 cut(s)	139
AfiI	CCNNNNNNNGG	5 cut(s)	246, 355, 744, 839, 917
AgsI	TTSAA	2 cut(s)	196, 632
AjnI	CCWGG	2 cut(s)	624, 910
AjuI	GAANNNNNNNTTGG	2 cut(s)	864, 896
AluBI	AGCT	1 cut(s)	469
AluI	AGCT	1 cut(s)	469
Alw26I	GTCTC	1 cut(s)	861
AlwI	GGATC	5 cut(s)	278, 462, 582, 595, 687
Ama87I	CYCGRG	1 cut(s)	864
AoxI	GGCC	2 cut(s)	3, 511
ApeKI	GCWGC	2 cut(s)	56, 436
ApoI	RAATTY	1 cut(s)	633
AseI	ATTAAT	1 cut(s)	321
AspLEI	GCGC	1 cut(s)	72
AspS9I	GGNCC	3 cut(s)	4, 237, 842
AsuHPI	GGTGA	3 cut(s)	197, 290, 338
AvaI	CYCGRG	1 cut(s)	864
AvaII	GGWCC	2 cut(s)	237, 842
BaeGI	GKGCMC	1 cut(s)	293
BamHI	GGATCC	1 cut(s)	587
BanII	GRGCYC	1 cut(s)	94
BbvI	GCAGC	2 cut(s)	43, 423
BccI	CCATC	4 cut(s)	15, 278, 522, 578
BciT130I	CCWGG	2 cut(s)	626, 912
BclI	TGATCA	3 cut(s)	223, 259, 385
BcoDI	GTCTC	1 cut(s)	861
BfaI	CTAG	1 cut(s)	329
BglII	AGATCT	1 cut(s)	500
BisI	GCNGC	3 cut(s)	57, 437, 511
BlsI	GCNGC	3 cut(s)	58, 438, 512
Bme1390I	CCNGG	2 cut(s)	626, 912
Bme18I	GGWCC	2 cut(s)	237, 842
BmeT110I	CYCGRG	1 cut(s)	864
BmgT120I	GGNCC	3 cut(s)	4, 237, 842
BmiI	GGNNCC	4 cut(s)	419, 589, 877, 901
BmrFI	CCNGG	2 cut(s)	626, 912
BmrI	ACTGGG	1 cut(s)	55
BmsI	GCATC	3 cut(s)	553, 622, 700
BmuI	ACTGGG	1 cut(s)	55
BpmI	CTGGAG	1 cut(s)	668
BpuEI	CTTGAG	1 cut(s)	499
BsaHI	GRCGYC	1 cut(s)	139
BsaJI	CCNNGG	4 cut(s)	240, 450, 508, 624
Bsc4I	CCNNNNNNNGG	5 cut(s)	246, 355, 744, 839, 917
Bse1I	ACTGG	3 cut(s)	61, 437, 685
Bse3DI	GCAATG	1 cut(s)	730
BseBI	CCWGG	2 cut(s)	626, 912
BseDI	CCNNGG	4 cut(s)	240, 450, 508, 624
BseGI	GGATG	2 cut(s)	544, 691
BseLI	CCNNNNNNNGG	5 cut(s)	246, 355, 744, 839, 917
BseMI	GCAATG	1 cut(s)	730
BseMII	CTCAG	1 cut(s)	791
BseNI	ACTGG	3 cut(s)	61, 437, 685
BseSI	GKGCMC	1 cut(s)	293
BseXI	GCAGC	2 cut(s)	43, 423
BsgI	GTGCAG	1 cut(s)	769
Bsh1236I	CGCG	1 cut(s)	510
BshFI	GGCC	2 cut(s)	5, 513
BsiHKCI	CYCGRG	1 cut(s)	864
BslI	CCNNNNNNNGG	5 cut(s)	246, 355, 744, 839, 917
BsmAI	GTCTC	1 cut(s)	861
BsmBI	CGTCTC	1 cut(s)	861
BsmI	GAATGC	1 cut(s)	403
BsnI	GGCC	2 cut(s)	5, 513
BsoBI	CYCGRG	1 cut(s)	864
Bsp1286I	GDGCHC	2 cut(s)	94, 293
Bsp19I	CCATGG	1 cut(s)	240
BspACI	CCGC	3 cut(s)	508, 510, 926
BspANI	GGCC	2 cut(s)	5, 513
BspCNI	CTCAG	1 cut(s)	790
BspFNI	CGCG	1 cut(s)	510
BspHI	TCATGA	1 cut(s)	220
BspLI	GGNNCC	4 cut(s)	419, 589, 877, 901
BspPI	GGATC	5 cut(s)	278, 462, 582, 595, 687
BsrDI	GCAATG	1 cut(s)	730
BsrI	ACTGG	3 cut(s)	61, 437, 685
BssECI	CCNNGG	4 cut(s)	240, 450, 508, 624
BssNI	GRCGYC	1 cut(s)	139
BssT1I	CCWWGG	2 cut(s)	240, 450
Bst2UI	CCWGG	2 cut(s)	626, 912
Bst4CI	ACNGT	3 cut(s)	656, 673, 818
Bst6I	CTCTTC	1 cut(s)	302
BstACI	GRCGYC	1 cut(s)	139
BstDEI	CTNAG	2 cut(s)	497, 777
BstDSI	CCRYGG	2 cut(s)	240, 508
BstF5I	GGATG	2 cut(s)	544, 691
BstFNI	CGCG	1 cut(s)	510
BstHHI	GCGC	1 cut(s)	72
BstMAI	GTCTC	1 cut(s)	861
BstMWI	GCNNNNNNNGC	2 cut(s)	610, 729
BstNI	CCWGG	2 cut(s)	626, 912
BstSCI	CCNGG	2 cut(s)	624, 910
BstSLI	GKGCMC	1 cut(s)	293
BstUI	CGCG	1 cut(s)	510
BstV1I	GCAGC	2 cut(s)	43, 423
BstX2I	RGATCY	4 cut(s)	270, 500, 587, 679
BstYI	RGATCY	4 cut(s)	270, 500, 587, 679
BsuRI	GGCC	2 cut(s)	5, 513
BtgI	CCRYGG	2 cut(s)	240, 508
BtsCI	GGATG	2 cut(s)	544, 691
BtsIMutI	CAGTG	2 cut(s)	577, 692
CciI	TCATGA	1 cut(s)	220
CfoI	GCGC	1 cut(s)	72
Cfr13I	GGNCC	3 cut(s)	4, 237, 842
Cfr42I	CCGCGG	1 cut(s)	511
CseI	GACGC	1 cut(s)	128
CsiI	ACCWGGT	1 cut(s)	910
CviAII	CATG	7 cut(s)	200, 221, 241, 316, 350, 811, 834
CviJI	RGCY	9 cut(s)	5, 92, 153, 436, 469, 485, 513, 539, 604
CviKI_1	RGCY	9 cut(s)	5, 92, 153, 436, 469, 485, 513, 539, 604
DdeI	CTNAG	2 cut(s)	497, 777
EaeI	YGGCCR	1 cut(s)	511
Eam1104I	CTCTTC	1 cut(s)	302
EarI	CTCTTC	1 cut(s)	302
Eco130I	CCWWGG	2 cut(s)	240, 450
Eco24I	GRGCYC	1 cut(s)	94
Eco47I	GGWCC	2 cut(s)	237, 842
Eco88I	CYCGRG	1 cut(s)	864
EcoRII	CCWGG	2 cut(s)	624, 910
EcoT14I	CCWWGG	2 cut(s)	240, 450
EcoT38I	GRGCYC	1 cut(s)	94
ErhI	CCWWGG	2 cut(s)	240, 450
Esp3I	CGTCTC	1 cut(s)	861
FaeI	CATG	7 cut(s)	203, 224, 244, 319, 353, 814, 837
FatI	CATG	7 cut(s)	199, 220, 240, 315, 349, 810, 833
FauNDI	CATATG	1 cut(s)	705
FbaI	TGATCA	3 cut(s)	223, 259, 385
Fnu4HI	GCNGC	3 cut(s)	57, 437, 511
FokI	GGATG	2 cut(s)	531, 678
FriOI	GRGCYC	1 cut(s)	94
Fsp4HI	GCNGC	3 cut(s)	57, 437, 511
FspBI	CTAG	1 cut(s)	329
GlaI	GCGC	1 cut(s)	71
GluI	GCNGC	3 cut(s)	57, 437, 511
GsuI	CTGGAG	1 cut(s)	668
HaeIII	GGCC	2 cut(s)	5, 513
HgaI	GACGC	1 cut(s)	128
HhaI	GCGC	1 cut(s)	72
Hin1I	GRCGYC	1 cut(s)	139
Hin1II	CATG	7 cut(s)	203, 224, 244, 319, 353, 814, 837
Hin6I	GCGC	1 cut(s)	70
HinP1I	GCGC	1 cut(s)	70
HindIII	AAGCTT	1 cut(s)	467
HinfI	GANTC	5 cut(s)	188, 211, 338, 353, 781
HphI	GGTGA	3 cut(s)	197, 290, 338
Hpy166II	GTNNAC	1 cut(s)	171
Hpy188I	TCNGA	2 cut(s)	519, 780
Hpy188III	TCNNGA	5 cut(s)	178, 221, 263, 866, 888
Hpy8I	GTNNAC	1 cut(s)	171
HpyAV	CCTTC	3 cut(s)	138, 484, 866
HpyCH4III	ACNGT	3 cut(s)	656, 673, 818
HpyCH4V	TGCA	4 cut(s)	78, 403, 644, 750
HpyF10VI	GCNNNNNNNGC	2 cut(s)	610, 729
HpyF3I	CTNAG	2 cut(s)	497, 777
Hsp92I	GRCGYC	1 cut(s)	139
Hsp92II	CATG	7 cut(s)	203, 224, 244, 319, 353, 814, 837
HspAI	GCGC	1 cut(s)	70
Ksp22I	TGATCA	3 cut(s)	223, 259, 385
KspI	CCGCGG	1 cut(s)	511
Lsp1109I	GCAGC	2 cut(s)	43, 423
LweI	GCATC	3 cut(s)	553, 622, 700
MabI	ACCWGGT	1 cut(s)	910
MaeI	CTAG	1 cut(s)	329
MaeIII	GTNAC	6 cut(s)	311, 428, 526, 656, 806, 836
MboII	GAAGA	4 cut(s)	24, 93, 319, 569
MflI	RGATCY	4 cut(s)	270, 500, 587, 679
MhlI	GDGCHC	2 cut(s)	94, 293
MluCI	AATT	7 cut(s)	82, 203, 394, 633, 790, 820, 921
MlyI	GAGTC	1 cut(s)	197
MmeI	TCCRAC	2 cut(s)	877, 898
MnlI	CCTC	5 cut(s)	129, 201, 474, 558, 884
MseI	TTAA	4 cut(s)	39, 321, 465, 931
MspA1I	CMGCKG	1 cut(s)	510
MspR9I	CCNGG	2 cut(s)	626, 912
Mva1269I	GAATGC	1 cut(s)	403
MvaI	CCWGG	2 cut(s)	626, 912
MvnI	CGCG	1 cut(s)	510
MwoI	GCNNNNNNNGC	2 cut(s)	610, 729
NcoI	CCATGG	1 cut(s)	240
NdeI	CATATG	1 cut(s)	705
NlaIII	CATG	7 cut(s)	203, 224, 244, 319, 353, 814, 837
NlaIV	GGNNCC	4 cut(s)	419, 589, 877, 901
NmuCI	GTSAC	3 cut(s)	526, 806, 836
PaeR7I	CTCGAG	1 cut(s)	864
PagI	TCATGA	1 cut(s)	220
PctI	GAATGC	1 cut(s)	403
PfeI	GAWTC	4 cut(s)	211, 338, 353, 781
PflMI	CCANNNNNTGG	2 cut(s)	246, 744
PkrI	GCNGC	3 cut(s)	58, 438, 512
PleI	GAGTC	1 cut(s)	196
PpsI	GAGTC	1 cut(s)	196
PshBI	ATTAAT	1 cut(s)	321
Psp6I	CCWGG	2 cut(s)	624, 910
PspGI	CCWGG	2 cut(s)	624, 910
PspN4I	GGNNCC	4 cut(s)	419, 589, 877, 901
PspPI	GGNCC	3 cut(s)	4, 237, 842
PsuI	RGATCY	4 cut(s)	270, 500, 587, 679
SacII	CCGCGG	1 cut(s)	511
SaqAI	TTAA	4 cut(s)	39, 321, 465, 931
SatI	GCNGC	3 cut(s)	57, 437, 511
Sau96I	GGNCC	3 cut(s)	4, 237, 842
SchI	GAGTC	1 cut(s)	197
ScrFI	CCNGG	2 cut(s)	626, 912
SduI	GDGCHC	2 cut(s)	94, 293
SetI	ASST	7 cut(s)	130, 239, 302, 471, 630, 895, 913
SexAI	ACCWGGT	1 cut(s)	910
SfaNI	GCATC	3 cut(s)	553, 622, 700
Sfr274I	CTCGAG	1 cut(s)	864
Sfr303I	CCGCGG	1 cut(s)	511
SgrBI	CCGCGG	1 cut(s)	511
SinI	GGWCC	2 cut(s)	237, 842
SlaI	CTCGAG	1 cut(s)	864
SmlI	CTYRAG	2 cut(s)	478, 864
SmoI	CTYRAG	2 cut(s)	478, 864
Sse9I	AATT	7 cut(s)	82, 203, 394, 633, 790, 820, 921
SsiI	CCGC	3 cut(s)	508, 510, 926
SspMI	CTAG	1 cut(s)	329
StyD4I	CCNGG	2 cut(s)	624, 910
StyI	CCWWGG	2 cut(s)	240, 450
TaaI	ACNGT	3 cut(s)	656, 673, 818
TaqI	TCGA	4 cut(s)	35, 104, 112, 865
TaqII	GACCGA	1 cut(s)	346
TasI	AATT	7 cut(s)	82, 203, 394, 633, 790, 820, 921
TauI	GCSGC	1 cut(s)	513
TfiI	GAWTC	4 cut(s)	211, 338, 353, 781
Tru1I	TTAA	4 cut(s)	39, 321, 465, 931
Tru9I	TTAA	4 cut(s)	39, 321, 465, 931
TscAI	CASTG	2 cut(s)	577, 692
TseFI	GTSAC	3 cut(s)	526, 806, 836
TseI	GCWGC	2 cut(s)	56, 436
Tsp45I	GTSAC	3 cut(s)	526, 806, 836
TspDTI	ATGAA	5 cut(s)	216, 351, 366, 503, 827
TspGWI	ACGGA	1 cut(s)	855
TspRI	CASTG	2 cut(s)	577, 692
Van91I	CCANNNNNTGG	2 cut(s)	246, 744
VpaK11BI	GGWCC	2 cut(s)	237, 842
VspI	ATTAAT	1 cut(s)	321
XapI	RAATTY	1 cut(s)	633
XcmI	CCANNNNNNNNNTGG	1 cut(s)	605
XhoI	CTCGAG	1 cut(s)	864
XspI	CTAG	1 cut(s)	329

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.