Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0000356

Physical Location & Seq

Genomic Coordinates Reverse (-)

58448773 .. 58459781

Gene Structure 11009 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00326390.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2193 bp

ATGATGTACCCTGATCCTCAACAGCAGCAGCAACACCAACAACAGCAGCAGCAGCAGCAACAACAACACTATCACCAGCAGCAACAGCAACACCACCAACACCAGCAGCAACACCATCAGCATCAGCAGCAAGGTGGAGACTTTCACAGGGGCCCACCGCCGCAGCAGCCGCCGCCGATGATGCGTCAGCCGTCTGCTTCTTCTACTAACATGGCTCCGGATTATCACCACCCCTCCGCCCCTGGTCCTCCTGTTCCTCCTTATGATGTTCATGGTGATGGTTTTGGTGCAAAGAGGAACAGAAAGCAGACTCAGAGAAGGGCAGTTGATTACACTAGCACTGTCGTGCGGTATACACAGACTCGAATGTGGCAGCGGGATGCGAGGGATAGGACAGTATTGCAACCTACACCAGCAGCTGCAATTGACATGTTGCCGACAGTCGCCTATTCAGATAACCCATCTACGGGCTTTGCTGCAAAGTTTGTGCATACTTCCACAAACAAAGTCCGTACTTCAATTAATCGGGTTTTGTGGACTCCTCCTGGGAGGCGTCTTATTACAGGCTCTCAAAATGGGGAATTCACTCTTTGGAACGGCCAATCATTTAATTTTGAAATGATTCTTCAGGCTCATGATCAAGCAATCAGATCTATGGTCTGGAGTCATAATGAAAATTGGATGGTCTCTGGTGATGATGGAGGATCAATAAAGTATTGGCAGACCAACATGAATAATGTGAAGGCCAATAAATCTGCTCACAAAGAATCTGTTCAGGACTTAAGCTTCTGTAAGACTGACTTGAAGTTCTGTTCCTGTTCGAATGATACTTTTGTTAAAATATGGGATTTTGCACGGTGCCAAGAAGAGCGCACATTGACTGGCCATGGTTGGGATGTGAAGAGTGTTGACTGGCACCCTACAAAGTCTCTACTAGCTTCAGGTGGGAAAGACCAGCTTGTCAAACTTTGGGATGCTAAGTCAGGGCGAGAACTATGTTCATTTCATGGTCACAAAAATTTGGTGCAATCTGTTAAATGGAACCAAAATGGCAACTGGCTGCTAACTGCTTCTAAGGATCAAGTCATAAAGCTTTACGACATAAGGGCTATGAAGGAGCTTGAATCTTTCCGTGGGCATCGGAAGGAAGTGACTGCTCTGGCTTGGCATCCTTTTCATGAAGAATATTTTGTCAGTGGGAGTTCTGATGGATCCATTTTCCATTGGCTTGTTGGGCATGAGACTCCCCAGGTTGAAATTGCTAATGCACACGATAACAGTGTGTGGGATCTCCAATGGCACCCTATTGGCTATATTCTTTGCAGGACATCCCCTTCTCAAGAAAATGAGGATTCCTTTCCAGAACGTGCAGTCTCTTGTCCAGACACATGTTATGGTGATCAAAACTCTGCTTTTGCTGGTCGCATGACGGGTAATTTTCCATTACACGATGGACCACCAACTCCAGGACCATTCACTCCCGGGGGAACTCGAAATGAAGGAACCATTCCAGGGGTTGGAGTAGCTATGCCGTTAGCCATACCATCCCTTGACGCATCTATTCAAGGAGAACAACAGCAACCTCATGCACATTCAATGCCTTTAGGAGCTCCTCCTCTCCCGCCTGGTCCACATCCTTCTCTTCTTGCTGCAAATCAGCAGCAACCATATCATCAAAATGCGCAACAGCAACATCAGCAACACCAAGGTCACCCACAACAAATGCCCCCTTTGCCTATGCCACCTCCAAATATGCCACAGCTACAGCCTCCATCCCATTTACCCTTGCTGCCGCATCCTCATTTACGTCCACCACCCCAAATGCCCCCACTCGGTATGCCATCTTCAGTGTCCGGATCTTTGCCTATGCCTTCATCAGTTCCAACATCACTTTCAATGCCAATGGGCATGCAAGGTACAATGAATCAGATGGTTCATCAATTGCCACAAGGTCATTATATGGGCATGAACCCACCTCCACCTGCTGGAGGTTTCCCAAATGGGATGCCGAACATGCAGGGCCCATCTAATGCAAGTGGAGGGCAACTGTATTCTCATGCTGGCACCTTCAATCGTGCACAATCTGGACAAATGCCAATGCTACCGGGGTATAATCCCTACCAGCCCAGGGGTCAATCCGGTATGCCTCAGCCTCCACCAGGGCCACCACCACATGGGCAAACACCTCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

WD40

Protein Analysis

730

Amino Acids

81.32

Weight (kDa)

7.82

Isoelectric Point (pI)

57.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_MABP1-WDR62_2nd	PF24782	122 - 409	4.2e-13	MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz	PF24807	156 - 335	2.1e-25	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd	PF25172	164 - 242	6.1e-06	WDR3 second beta-propeller domain
WDR55	PF24796	165 - 284	3.8e-10	WDR55
Beta-prop_WDR3_1st	PF25173	168 - 319	1.8e-23	WDR3 first beta-propeller domain
Beta-prop_EIPR1	PF23609	169 - 324	4.2e-06	EIPR1 beta-propeller
Beta-prop_WDR5	PF25175	170 - 294	2.5e-18	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	170 - 341	7.3e-26	THOC3 beta-propeller domain
Beta-prop_IFT140_1st	PF23383	171 - 259	1.9e-09	IFT140 first beta-propeller
WD40_Gbeta	PF25391	174 - 377	7.5e-20	G protein beta WD-40 repeat protein
WD40_WDHD1_1st	PF24817	175 - 335	4.7e-15	WDHD1 first WD40 domain
Beta-prop_EML	PF23409	181 - 249	1.6e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19	PF24814	183 - 376	4.1e-31	Prp19 WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	205 - 312	8.4e-07	CAF1B/HIR1 beta-propeller domain
WD40	PF00400	207 - 240	1e-05	WD domain, G-beta repeat
EIF3I	PF24805	208 - 289	3.3e-08	EIF3I
Beta-prop_TEP1_2nd	PF25047	217 - 415	1.1e-16	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	236 - 325	1.7e-09	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	249 - 410	2.5e-18	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2	PF23414	265 - 434	5.7e-20	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	287 - 409	1.4e-27	WDR3 first beta-propeller domain
WD40	PF00400	289 - 325	4.3e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	291 - 373	1e-11	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5	PF25175	292 - 444	1.1e-26	WDR5 beta-propeller domain
EIF3I	PF24805	292 - 440	1.3e-11	EIF3I
Beta-prop_EML	PF23409	293 - 399	5.3e-09	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd	PF25172	293 - 412	9.4e-14	WDR3 second beta-propeller domain
Beta-prop_IFT140_1st	PF23383	295 - 363	5e-06	IFT140 first beta-propeller
Beta-prop_Aladin	PF25460	299 - 414	1.8e-06	Aladin seven-bladed propeller
WDR55	PF24796	300 - 435	1.3e-11	WDR55
Beta-prop_WDR19_1st	PF23389	303 - 434	3.1e-06	WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	320 - 440	2.9e-08	WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz	PF24807	330 - 441	5.1e-17	CDC20/Fizzy WD40 domain
WD40	PF00400	330 - 367	5.8e-08	WD domain, G-beta repeat
ANAPC4_WD40	PF12894	337 - 390	9e-06	Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_THOC3	PF25174	338 - 444	1.1e-18	THOC3 beta-propeller domain
Beta-prop_EIPR1	PF23609	338 - 406	2.1e-06	EIPR1 beta-propeller
WD40_WDHD1_1st	PF24817	342 - 419	2.5e-10	WDHD1 first WD40 domain
WD40_Prp19	PF24814	372 - 443	2.1e-07	Prp19 WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000356)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca	FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica	MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica	Prupe.2G034300_v2.0.a1
pyrus_communis	pycom02g24420 pycom07g02650
rosa_chinensis	RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata	RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora	Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii	Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa	Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha	Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana	Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1990
AasI	GACNNNNNNGTC	1 cut(s)	959
Acc16I	TGCGCA	1 cut(s)	1683
Acc36I	ACCTGC	1 cut(s)	1990
AccB1I	GGYRCC	4 cut(s)	858, 915, 1299, 2063
AccB7I	CCANNNNNTGG	3 cut(s)	1517, 1985, 2174
AccI	GTMKAC	1 cut(s)	353
AccIII	TCCGGA	2 cut(s)	217, 1853
AciI	CCGC	9 cut(s)	158, 161, 170, 173, 237, 349, 376, 1622, 1793
AclWI	GGATC	7 cut(s)	8, 712, 1086, 1206, 1219, 1296, 1864
AcoI	YGGCCR	2 cut(s)	598, 883
AcsI	RAATTY	2 cut(s)	581, 1018
AcuI	CTGAAG	3 cut(s)	611, 924, 1830
AcyI	GRCGYC	1 cut(s)	553
AfaI	GTAC	3 cut(s)	8, 514, 1918
AflII	CTTAAG	1 cut(s)	781
AflIII	ACRYGT	2 cut(s)	429, 1388
AgsI	TTSAA	9 cut(s)	519, 617, 805, 1124, 1256, 1565, 1596, 1896, 2071
AjnI	CCWGG	8 cut(s)	241, 544, 1248, 1465, 1510, 1624, 2126, 2158
AjuI	GAANNNNNNNTTGG	2 cut(s)	1876, 1908
AleI	CACNNNNGTG	1 cut(s)	344
AluBI	AGCT	9 cut(s)	419, 786, 938, 958, 1093, 1120, 1526, 1610, 1762
AluI	AGCT	9 cut(s)	419, 786, 938, 958, 1093, 1120, 1526, 1610, 1762
Alw21I	GWGCWC	2 cut(s)	1612, 2080
Alw26I	GTCTC	5 cut(s)	132, 691, 933, 1235, 1378
Alw44I	GTGCAC	1 cut(s)	2076
AlwI	GGATC	7 cut(s)	8, 712, 1086, 1206, 1219, 1296, 1864
AlwNI	CAGNNNCTG	1 cut(s)	419
Ama87I	CYCGRG	1 cut(s)	1481
Aor13HI	TCCGGA	2 cut(s)	217, 1853
AoxI	GGCC	6 cut(s)	151, 598, 744, 883, 2020, 2162
ApaI	GGGCCC	2 cut(s)	155, 2024
ApaLI	GTGCAC	1 cut(s)	2076
ApoI	RAATTY	2 cut(s)	581, 1018
AseI	ATTAAT	1 cut(s)	522
Asp700I	GAANNNNTTC	2 cut(s)	621, 771
AspLEI	GCGC	2 cut(s)	873, 1684
AspS9I	GGNCC	9 cut(s)	151, 152, 245, 1454, 1469, 1628, 2020, 2021, 2162
AsuC2I	CCSGG	3 cut(s)	1482, 1483, 2106
AsuHPI	GGTGA	6 cut(s)	65, 218, 287, 704, 1409, 1703
AsuII	TTCGAA	1 cut(s)	821
AvaI	CYCGRG	1 cut(s)	1481
AvaII	GGWCC	4 cut(s)	245, 1454, 1469, 1628
BaeGI	GKGCMC	3 cut(s)	155, 2024, 2080
BaeI	ACNNNNGTAYC	2 cut(s)	819, 852
BalI	TGGCCA	1 cut(s)	885
BamHI	GGATCC	1 cut(s)	1211
BanI	GGYRCC	4 cut(s)	858, 915, 1299, 2063
BanII	GRGCYC	3 cut(s)	155, 1612, 2024
BarI	GAAGNNNNNNTAC	2 cut(s)	1828, 1860
Bbv12I	GWGCWC	2 cut(s)	1612, 2080
BbvCI	CCTCAGC	1 cut(s)	2148
BceAI	ACGGC	3 cut(s)	175, 613, 1516
BciT130I	CCWGG	8 cut(s)	243, 546, 1250, 1467, 1512, 1626, 2128, 2160
BclI	TGATCA	2 cut(s)	637, 1399
BcnI	CCSGG	3 cut(s)	1482, 1483, 2106
BcoDI	GTCTC	5 cut(s)	132, 691, 933, 1235, 1378
BfaI	CTAG	2 cut(s)	336, 935
BfmI	CTRYAG	1 cut(s)	1763
BfrI	CTTAAG	1 cut(s)	781
BfuAI	ACCTGC	1 cut(s)	1990
BglII	AGATCT	1 cut(s)	650
Bme18I	GGWCC	4 cut(s)	245, 1454, 1469, 1628
BmeT110I	CYCGRG	1 cut(s)	1481
BmgT120I	GGNCC	9 cut(s)	151, 152, 245, 1454, 1469, 1628, 2020, 2021, 2162
BmsI	GCATC	9 cut(s)	130, 171, 370, 964, 1147, 1177, 1565, 1804, 1995
BpmI	CTGGAG	3 cut(s)	682, 1449, 2007
Bpu10I	CCTNAGC	1 cut(s)	2148
Bpu14I	TTCGAA	1 cut(s)	821
BpuEI	CTTGAG	1 cut(s)	1323
BpuMI	CCSGG	3 cut(s)	1482, 1483, 2106
BsaHI	GRCGYC	1 cut(s)	553
BsaI	GGTCTC	1 cut(s)	691
BsaWI	WCCGGW	3 cut(s)	217, 1853, 2138
BsaXI	ACNNNNNCTCC	2 cut(s)	218, 248
Bse1I	ACTGG	3 cut(s)	886, 917, 1061
BseAI	TCCGGA	2 cut(s)	217, 1853
BseBI	CCWGG	8 cut(s)	243, 546, 1250, 1467, 1512, 1626, 2128, 2160
BseMII	CTCAG	2 cut(s)	326, 2162
BseNI	ACTGG	3 cut(s)	886, 917, 1061
BseRI	GAGGAG	3 cut(s)	531, 1602, 1605
BseSI	GKGCMC	3 cut(s)	155, 2024, 2080
BsgI	GTGCAG	1 cut(s)	1389
BshFI	GGCC	6 cut(s)	153, 600, 746, 885, 2022, 2164
BshNI	GGYRCC	4 cut(s)	858, 915, 1299, 2063
BsiHKAI	GWGCWC	2 cut(s)	1612, 2080
BsiHKCI	CYCGRG	1 cut(s)	1481
BsiSI	CCGG	5 cut(s)	218, 1482, 1854, 2105, 2139
BsmAI	GTCTC	5 cut(s)	132, 691, 933, 1235, 1378
BsnI	GGCC	6 cut(s)	153, 600, 746, 885, 2022, 2164
Bso31I	GGTCTC	1 cut(s)	691
BsoBI	CYCGRG	1 cut(s)	1481
Bsp119I	TTCGAA	1 cut(s)	821
Bsp120I	GGGCCC	2 cut(s)	151, 2020
Bsp1286I	GDGCHC	4 cut(s)	155, 1612, 2024, 2080
Bsp13I	TCCGGA	2 cut(s)	217, 1853
Bsp143I	GATC	9 cut(s)	13, 637, 650, 704, 1078, 1211, 1288, 1399, 1856
Bsp19I	CCATGG	1 cut(s)	886
BspACI	CCGC	9 cut(s)	158, 161, 170, 173, 237, 349, 376, 1622, 1793
BspANI	GGCC	6 cut(s)	153, 600, 746, 885, 2022, 2164
BspCNI	CTCAG	2 cut(s)	325, 2161
BspEI	TCCGGA	2 cut(s)	217, 1853
BspHI	TCATGA	2 cut(s)	634, 1177
BspMI	ACCTGC	1 cut(s)	1990
BspPI	GGATC	7 cut(s)	8, 712, 1086, 1206, 1219, 1296, 1864
BspQI	GCTCTTC	1 cut(s)	861
BspT104I	TTCGAA	1 cut(s)	821
BspT107I	GGYRCC	4 cut(s)	858, 915, 1299, 2063
BspTI	CTTAAG	1 cut(s)	781
BspTNI	GGTCTC	1 cut(s)	691
BsrI	ACTGG	3 cut(s)	886, 917, 1061
BssMI	GATC	9 cut(s)	13, 637, 650, 704, 1078, 1211, 1288, 1399, 1856
BssNAI	GTATAC	1 cut(s)	354
BssNI	GRCGYC	1 cut(s)	553
BssT1I	CCWWGG	2 cut(s)	886, 1705
Bst1107I	GTATAC	1 cut(s)	354
Bst2UI	CCWGG	8 cut(s)	243, 546, 1250, 1467, 1512, 1626, 2128, 2160
Bst4CI	ACNGT	6 cut(s)	343, 397, 442, 858, 1280, 2049
Bst6I	CTCTTC	3 cut(s)	861, 896, 1647
BstACI	GRCGYC	1 cut(s)	553
BstAFI	CTTAAG	1 cut(s)	781
BstBI	TTCGAA	1 cut(s)	821
BstC8I	GCNNGC	2 cut(s)	1910, 2062
BstDEI	CTNAG	4 cut(s)	312, 978, 1074, 2148
BstDSI	CCRYGG	2 cut(s)	886, 1132
BstEII	GGTNACC	1 cut(s)	1709
BstENI	CCTNNNNNAGG	2 cut(s)	1986, 2157
BstHHI	GCGC	2 cut(s)	873, 1684
BstKTI	GATC	9 cut(s)	16, 640, 653, 707, 1081, 1214, 1291, 1402, 1859
BstMAI	GTCTC	5 cut(s)	132, 691, 933, 1235, 1378
BstMBI	GATC	9 cut(s)	13, 637, 650, 704, 1078, 1211, 1288, 1399, 1856
BstNI	CCWGG	8 cut(s)	243, 546, 1250, 1467, 1512, 1626, 2128, 2160
BstNSI	RCATGY	4 cut(s)	433, 1392, 1912, 2017
BstPI	GGTNACC	1 cut(s)	1709
BstSFI	CTRYAG	1 cut(s)	1763
BstSLI	GKGCMC	3 cut(s)	155, 2024, 2080
BstX2I	RGATCY	4 cut(s)	650, 1211, 1288, 1856
BstYI	RGATCY	4 cut(s)	650, 1211, 1288, 1856
BstZ17I	GTATAC	1 cut(s)	354
BsuRI	GGCC	6 cut(s)	153, 600, 746, 885, 2022, 2164
BtgI	CCRYGG	2 cut(s)	886, 1132
BtsIMutI	CAGTG	4 cut(s)	339, 1201, 1285, 1854
BveI	ACCTGC	1 cut(s)	1990
Cac8I	GCNNGC	2 cut(s)	1910, 2062
CaiI	CAGNNNCTG	1 cut(s)	419
CciI	TCATGA	2 cut(s)	634, 1177
CfoI	GCGC	2 cut(s)	873, 1684
Cfr13I	GGNCC	9 cut(s)	151, 152, 245, 1454, 1469, 1628, 2020, 2021, 2162
Cfr9I	CCCGGG	1 cut(s)	1481
CseI	GACGC	3 cut(s)	173, 542, 1562
Csp6I	GTAC	3 cut(s)	7, 513, 1917
CviQI	GTAC	3 cut(s)	7, 513, 1917
DdeI	CTNAG	4 cut(s)	312, 978, 1074, 2148
DpnI	GATC	9 cut(s)	15, 639, 652, 706, 1080, 1213, 1290, 1401, 1858
DpnII	GATC	9 cut(s)	13, 637, 650, 704, 1078, 1211, 1288, 1399, 1856
DrdI	GACNNNNNNGTC	1 cut(s)	959
DseDI	GACNNNNNNGTC	1 cut(s)	959
EaeI	YGGCCR	2 cut(s)	598, 883
Eam1104I	CTCTTC	3 cut(s)	861, 896, 1647
EarI	CTCTTC	3 cut(s)	861, 896, 1647
EciI	GGCGGA	1 cut(s)	226
Ecl136II	GAGCTC	1 cut(s)	1610
Eco130I	CCWWGG	2 cut(s)	886, 1705
Eco24I	GRGCYC	3 cut(s)	155, 1612, 2024
Eco31I	GGTCTC	1 cut(s)	691
Eco47I	GGWCC	4 cut(s)	245, 1454, 1469, 1628
Eco53kI	GAGCTC	1 cut(s)	1610
Eco57I	CTGAAG	3 cut(s)	611, 924, 1830
Eco88I	CYCGRG	1 cut(s)	1481
Eco91I	GGTNACC	1 cut(s)	1709
EcoICRI	GAGCTC	1 cut(s)	1610
EcoNI	CCTNNNNNAGG	2 cut(s)	1986, 2157
EcoO109I	RGGNCCY	2 cut(s)	151, 2020
EcoO65I	GGTNACC	1 cut(s)	1709
EcoRI	GAATTC	1 cut(s)	581
EcoRII	CCWGG	8 cut(s)	241, 544, 1248, 1465, 1510, 1624, 2126, 2158
EcoT14I	CCWWGG	2 cut(s)	886, 1705
EcoT38I	GRGCYC	3 cut(s)	155, 1612, 2024
ErhI	CCWWGG	2 cut(s)	886, 1705
FalI	AAGNNNNNCTT	4 cut(s)	785, 817, 942, 974
FauI	CCCGC	2 cut(s)	369, 1629
FbaI	TGATCA	2 cut(s)	637, 1399
FblI	GTMKAC	1 cut(s)	353
FriOI	GRGCYC	3 cut(s)	155, 1612, 2024
FspBI	CTAG	2 cut(s)	336, 935
FspI	TGCGCA	1 cut(s)	1683
GlaI	GCGC	2 cut(s)	872, 1683
GsuI	CTGGAG	3 cut(s)	682, 1449, 2007
HaeIII	GGCC	6 cut(s)	153, 600, 746, 885, 2022, 2164
HapII	CCGG	5 cut(s)	218, 1482, 1854, 2105, 2139
HgaI	GACGC	3 cut(s)	173, 542, 1562
HhaI	GCGC	2 cut(s)	873, 1684
Hin1I	GRCGYC	1 cut(s)	553
Hin6I	GCGC	2 cut(s)	871, 1682
HinP1I	GCGC	2 cut(s)	871, 1682
HincII	GTYRAC	1 cut(s)	910
HindII	GTYRAC	1 cut(s)	910
HindIII	AAGCTT	2 cut(s)	784, 1091
HpaII	CCGG	5 cut(s)	218, 1482, 1854, 2105, 2139
HphI	GGTGA	6 cut(s)	65, 218, 287, 704, 1409, 1703
Hpy166II	GTNNAC	6 cut(s)	354, 537, 910, 1631, 1811, 2078
Hpy188I	TCNGA	6 cut(s)	315, 454, 650, 1143, 1207, 1929
Hpy8I	GTNNAC	6 cut(s)	354, 537, 910, 1631, 1811, 2078
HpyAV	CCTTC	9 cut(s)	312, 736, 1108, 1138, 1344, 1493, 1647, 1881, 2077
HpyCH4III	ACNGT	6 cut(s)	343, 397, 442, 858, 1280, 2049
HpyCH4IV	ACGT	2 cut(s)	1366, 1807
HpyF3I	CTNAG	4 cut(s)	312, 978, 1074, 2148
HpySE526I	ACGT	2 cut(s)	1366, 1807
Hsp92I	GRCGYC	1 cut(s)	553
HspAI	GCGC	2 cut(s)	871, 1682
Kpn2I	TCCGGA	2 cut(s)	217, 1853
Ksp22I	TGATCA	2 cut(s)	637, 1399
Kzo9I	GATC	9 cut(s)	13, 637, 650, 704, 1078, 1211, 1288, 1399, 1856
LguI	GCTCTTC	1 cut(s)	861
LmnI	GCTCC	4 cut(s)	220, 1117, 1607, 1615
LweI	GCATC	9 cut(s)	130, 171, 370, 964, 1147, 1177, 1565, 1804, 1995
MaeI	CTAG	2 cut(s)	336, 935
MaeII	ACGT	2 cut(s)	1366, 1807
MaeIII	GTNAC	3 cut(s)	1010, 1150, 1709
MalI	GATC	9 cut(s)	15, 639, 652, 706, 1080, 1213, 1290, 1401, 1858
MboI	GATC	9 cut(s)	13, 637, 650, 704, 1078, 1211, 1288, 1399, 1856
MboII	GAAGA	7 cut(s)	192, 617, 878, 913, 1193, 1634, 1836
MfeI	CAATTG	2 cut(s)	423, 1940
MflI	RGATCY	4 cut(s)	650, 1211, 1288, 1856
MhlI	GDGCHC	4 cut(s)	155, 1612, 2024, 2080
MlsI	TGGCCA	1 cut(s)	885
MluCI	AATT	9 cut(s)	423, 519, 581, 610, 676, 1018, 1257, 1435, 1940
MluNI	TGGCCA	1 cut(s)	885
MlyI	GAGTC	5 cut(s)	304, 355, 532, 673, 1237
MmeI	TCCRAC	2 cut(s)	1498, 1907
Mox20I	TGGCCA	1 cut(s)	885
MroI	TCCGGA	2 cut(s)	217, 1853
MroXI	GAANNNNTTC	2 cut(s)	621, 771
MscI	TGGCCA	1 cut(s)	885
MseI	TTAA	5 cut(s)	522, 609, 782, 837, 1035
MslI	CAYNNNNRTG	3 cut(s)	276, 344, 1677
Msp20I	TGGCCA	1 cut(s)	885
MspA1I	CMGCKG	2 cut(s)	376, 419
MspCI	CTTAAG	1 cut(s)	781
MspI	CCGG	5 cut(s)	218, 1482, 1854, 2105, 2139
MunI	CAATTG	2 cut(s)	423, 1940
MvaI	CCWGG	8 cut(s)	243, 546, 1250, 1467, 1512, 1626, 2128, 2160
NciI	CCSGG	3 cut(s)	1482, 1483, 2106
NcoI	CCATGG	1 cut(s)	886
NdeII	GATC	9 cut(s)	13, 637, 650, 704, 1078, 1211, 1288, 1399, 1856
NmuCI	GTSAC	3 cut(s)	1010, 1150, 1709
NsbI	TGCGCA	1 cut(s)	1683
NspI	RCATGY	4 cut(s)	433, 1392, 1912, 2017
NspV	TTCGAA	1 cut(s)	821
OliI	CACNNNNGTG	1 cut(s)	344
PaeI	GCATGC	1 cut(s)	1912
PagI	TCATGA	2 cut(s)	634, 1177
PaqCI	CACCTGC	1 cut(s)	1990
PasI	CCCWGGG	1 cut(s)	2127
PciI	ACATGT	2 cut(s)	429, 1388
PciSI	GCTCTTC	1 cut(s)	861
PdmI	GAANNNNTTC	2 cut(s)	621, 771
PfeI	GAWTC	5 cut(s)	622, 767, 1124, 1352, 1924
PflMI	CCANNNNNTGG	3 cut(s)	1517, 1985, 2174
PfoI	TCCNGGA	1 cut(s)	1465
PleI	GAGTC	5 cut(s)	304, 355, 532, 672, 1237
PpsI	GAGTC	5 cut(s)	304, 355, 532, 672, 1237
PscI	ACATGT	2 cut(s)	429, 1388
PshBI	ATTAAT	1 cut(s)	522
Psp124BI	GAGCTC	1 cut(s)	1612
Psp6I	CCWGG	8 cut(s)	241, 544, 1248, 1465, 1510, 1624, 2126, 2158
PspEI	GGTNACC	1 cut(s)	1709
PspGI	CCWGG	8 cut(s)	241, 544, 1248, 1465, 1510, 1624, 2126, 2158
PspOMI	GGGCCC	2 cut(s)	151, 2020
PspPI	GGNCC	9 cut(s)	151, 152, 245, 1454, 1469, 1628, 2020, 2021, 2162
PstNI	CAGNNNCTG	1 cut(s)	419
PsuI	RGATCY	4 cut(s)	650, 1211, 1288, 1856
PvuII	CAGCTG	1 cut(s)	419
RsaI	GTAC	3 cut(s)	8, 514, 1918
RsaNI	GTAC	3 cut(s)	7, 513, 1917
RseI	CAYNNNNRTG	3 cut(s)	276, 344, 1677
SacI	GAGCTC	1 cut(s)	1612
SapI	GCTCTTC	1 cut(s)	861
SaqAI	TTAA	5 cut(s)	522, 609, 782, 837, 1035
Sau3AI	GATC	9 cut(s)	13, 637, 650, 704, 1078, 1211, 1288, 1399, 1856
Sau96I	GGNCC	9 cut(s)	151, 152, 245, 1454, 1469, 1628, 2020, 2021, 2162
SchI	GAGTC	5 cut(s)	304, 355, 532, 673, 1237
SduI	GDGCHC	4 cut(s)	155, 1612, 2024, 2080
SfaNI	GCATC	9 cut(s)	130, 171, 370, 964, 1147, 1177, 1565, 1804, 1995
SfcI	CTRYAG	1 cut(s)	1763
SfuI	TTCGAA	1 cut(s)	821
SinI	GGWCC	4 cut(s)	245, 1454, 1469, 1628
SmaI	CCCGGG	1 cut(s)	1483
SmiMI	CAYNNNNRTG	3 cut(s)	276, 344, 1677
SmlI	CTYRAG	2 cut(s)	781, 1338
SmoI	CTYRAG	2 cut(s)	781, 1338
SphI	GCATGC	1 cut(s)	1912
Sse9I	AATT	9 cut(s)	423, 519, 581, 610, 676, 1018, 1257, 1435, 1940
SsiI	CCGC	9 cut(s)	158, 161, 170, 173, 237, 349, 376, 1622, 1793
SspI	AATATT	1 cut(s)	1187
SspMI	CTAG	2 cut(s)	336, 935
SstI	GAGCTC	1 cut(s)	1612
StyI	CCWWGG	2 cut(s)	886, 1705
TaaI	ACNGT	6 cut(s)	343, 397, 442, 858, 1280, 2049
TaiI	ACGT	2 cut(s)	1369, 1810
TaqI	TCGA	3 cut(s)	364, 821, 1492
TasI	AATT	9 cut(s)	423, 519, 581, 610, 676, 1018, 1257, 1435, 1940
TauI	GCSGC	4 cut(s)	163, 172, 175, 1795
TfiI	GAWTC	5 cut(s)	622, 767, 1124, 1352, 1924
Tru1I	TTAA	5 cut(s)	522, 609, 782, 837, 1035
Tru9I	TTAA	5 cut(s)	522, 609, 782, 837, 1035
TscAI	CASTG	4 cut(s)	346, 1201, 1285, 1854
TseFI	GTSAC	3 cut(s)	1010, 1150, 1709
Tsp45I	GTSAC	3 cut(s)	1010, 1150, 1709
TspGWI	ACGGA	2 cut(s)	500, 1121
TspMI	CCCGGG	1 cut(s)	1481
TspRI	CASTG	4 cut(s)	346, 1201, 1285, 1854
Van91I	CCANNNNNTGG	3 cut(s)	1517, 1985, 2174
Vha464I	CTTAAG	1 cut(s)	781
VneI	GTGCAC	1 cut(s)	2076
VpaK11BI	GGWCC	4 cut(s)	245, 1454, 1469, 1628
VspI	ATTAAT	1 cut(s)	522
XagI	CCTNNNNNAGG	2 cut(s)	1986, 2157
XapI	RAATTY	2 cut(s)	581, 1018
XceI	RCATGY	4 cut(s)	433, 1392, 1912, 2017
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1229
XmaI	CCCGGG	1 cut(s)	1481
XmiI	GTMKAC	1 cut(s)	353
XmnI	GAANNNNTTC	2 cut(s)	621, 771
XspI	CTAG	2 cut(s)	336, 935

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.