Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0000356

Physical Location & Seq

Genomic Coordinates Forward (+)

10983158 .. 10991230

Gene Structure 8073 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG062300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1149 bp

ATGATGCGTCAGCCGTCTGCTTCTTCTACTAACATGCCTCCGGATTATCACCACCCCTCCGCCCCCGGTCCTCCTGTTCCTCCTTATGATGTTCATGGTGATGGTTTTGGTGCAAAGAGGAACAGAAAGCAGACTCAGAGAAGGGCAGTTGATTACACTAGCACTGTCGTGCGGTATACACAGACTAGAATGTGGCAGCGGGATGCGAGGGATAGGACAGTATTGCAACCTACACCAGCAGCAGCAATTGACATGTTGCCGACAGTTGCCTATTCAAATAACCCATCTACGGGCTTTGCTGCAAAGTTTGTGCATACTTCCACAAACAAAGTCCGTAATTCAATTAATCGGGTTTTGTGGACTCCTCCTGGGAGGCGTCTTATTACAGGCTCTCAAAATGGGGAATTCACTCTTTGGAATGGCCAATCATTTAATTTTGAAATGATTCTTCAGGCTCATGATCAAGCAATCAGATCTATGGTCTGGAGTCATAATGAAAATTGGATGGTCTCTGGTGATGATGGAGGATCAATAAAGTATTGGCAGACCAACATGAATAATGTGAAGGCCAATAAATCTGCTCACAAAGAATCTGTTCAGGACTTAAGCTTCTGTAAGACTGACTTGAAGTTCTGTTCCTGTTCGAATGATACTTCTGTTAAAATATGGGATTTTGCACGGTGCCAAGAAGAGCGCACATTGACTGGCCATGGTTGGGATGTGAAGAGTGTTGACTGGCACCCTACAAAGTCTCTACTAGCTTCAGGTGGGAAAGACCAGCTTGTCAAACTTTGGGATGCTAAGTCGGGGCGAGAACTATGTTCATTTCATGGTCACAAAAATCTGGTGCAATCTGTTAAATGGAACCAAAATGGCAACTGGCTGCTAACTGCTTCTAAGGATCAAGTCATAAAGCTTTATGACATAAGGGCTATGAAGGAGCTTGAATCTTTCCGTGGGCATCGGAAGGAAGTGACTGTGGTAGCAATGATCACACAACAAAGTTTTGGTGCAGAAACAGGCCAGGAGACACTGGTCGCGATAAATTTAACATCAATCAGAGTCAAGGTTCCACTCTTCCTAACCCAATATCAAATTGTTAAGACATTATCATCTGATTGCTTATCAAATAGAATCTCATTTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

WD40

Protein Analysis

382

Amino Acids

43.2

Weight (kDa)

9.54

Isoelectric Point (pI)

32.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	91 - 276	2.3e-26	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	105 - 178	4.2e-06	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	105 - 183	1e-06	WDR3 second beta-propeller domain
WDR55	PF24796	107 - 184	2.8e-06	WDR55
Beta-prop_WDR3_1st	PF25173	108 - 226	1e-17	WDR3 first beta-propeller domain
Beta-prop_EML_2	PF23414	108 - 194	6.3e-06	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EIPR1	PF23609	110 - 265	3.6e-06	EIPR1 beta-propeller
Beta-prop_THOC3	PF25174	110 - 324	1.6e-35	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	111 - 229	6.1e-18	WDR5 beta-propeller domain
Beta-prop_IFT140_1st	PF23383	114 - 200	9.5e-10	IFT140 first beta-propeller
WD40_Gbeta	PF25391	115 - 336	1.1e-21	G protein beta WD-40 repeat protein
Beta-prop_EML	PF23409	123 - 190	6.1e-07	Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19	PF24814	124 - 329	5.7e-33	Prp19 WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	146 - 248	5.6e-07	CAF1B/HIR1 beta-propeller domain
WD40	PF00400	148 - 181	4.4e-06	WD domain, G-beta repeat
EIF3I	PF24805	149 - 230	7.8e-09	EIF3I
WD40_MABP1-WDR62_2nd	PF24782	156 - 325	1.4e-10	MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd	PF25047	158 - 333	3.3e-16	TEP-1 second beta-propeller
WD40_WDHD1_1st	PF24817	171 - 276	4.1e-10	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	177 - 266	4.9e-10	WDR3 second beta-propeller domain
WDR55	PF24796	186 - 334	3.7e-15	WDR55
Beta-prop_Aladin	PF25460	190 - 344	1.9e-07	Aladin seven-bladed propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	190 - 340	1.3e-18	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5	PF25175	193 - 346	8.6e-32	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	206 - 328	2.1e-17	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	210 - 311	1.2e-08	WDR90/POC16, second beta-propeller
Beta-prop_WDR3_1st	PF25173	219 - 336	1.7e-27	WDR3 first beta-propeller domain
Beta-prop_IFT140_1st	PF23383	220 - 307	1.5e-06	IFT140 first beta-propeller
WD40	PF00400	230 - 266	1.9e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	232 - 317	2e-12	CAF1B/HIR1 beta-propeller domain
Beta-prop_EML	PF23409	234 - 330	2.5e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd	PF25172	234 - 343	8.4e-12	WDR3 second beta-propeller domain
EIF3I	PF24805	240 - 322	1e-09	EIF3I
WD40	PF00400	271 - 308	2.5e-08	WD domain, G-beta repeat
WD40_CDC20-Fz	PF24807	272 - 344	3.3e-08	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	279 - 334	1.1e-08	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000356)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca	FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica	MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica	Prupe.2G034300_v2.0.a1
pyrus_communis	pycom02g24420 pycom07g02650
rosa_chinensis	RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata	RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora	Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii	Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa	Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha	Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana	Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	782
AccB1I	GGYRCC	2 cut(s)	681, 738
AccI	GTMKAC	1 cut(s)	176
AccII	CGCG	1 cut(s)	1040
AccIII	TCCGGA	1 cut(s)	40
AciI	CCGC	3 cut(s)	60, 172, 199
AclWI	GGATC	2 cut(s)	535, 909
AcoI	YGGCCR	2 cut(s)	421, 706
AcsI	RAATTY	2 cut(s)	404, 1045
AcuI	CTGAAG	2 cut(s)	434, 747
AcyI	GRCGYC	1 cut(s)	376
AfiI	CCNNNNNNNGG	3 cut(s)	289, 290, 715
AflII	CTTAAG	1 cut(s)	604
AflIII	ACRYGT	1 cut(s)	252
AgsI	TTSAA	5 cut(s)	276, 342, 440, 628, 947
AjnI	CCWGG	2 cut(s)	367, 1023
AleI	CACNNNNGTG	1 cut(s)	167
AluBI	AGCT	5 cut(s)	609, 761, 781, 916, 943
AluI	AGCT	5 cut(s)	609, 761, 781, 916, 943
Alw26I	GTCTC	3 cut(s)	514, 756, 1022
AlwI	GGATC	2 cut(s)	535, 909
Aor13HI	TCCGGA	1 cut(s)	40
AoxI	GGCC	4 cut(s)	421, 567, 706, 1021
ApeKI	GCWGC	5 cut(s)	196, 239, 242, 299, 883
ApoI	RAATTY	2 cut(s)	404, 1045
AseI	ATTAAT	1 cut(s)	345
Asp700I	GAANNNNTTC	2 cut(s)	444, 594
AspLEI	GCGC	1 cut(s)	696
AspS9I	GGNCC	1 cut(s)	68
AsuC2I	CCSGG	1 cut(s)	66
AsuHPI	GGTGA	3 cut(s)	41, 110, 527
AsuII	TTCGAA	1 cut(s)	644
AvaII	GGWCC	1 cut(s)	68
BaeI	ACNNNNGTAYC	2 cut(s)	642, 675
BalI	TGGCCA	2 cut(s)	423, 708
BanI	GGYRCC	2 cut(s)	681, 738
BbvI	GCAGC	5 cut(s)	208, 251, 254, 286, 870
BccI	CCATC	4 cut(s)	95, 292, 499, 515
BciT130I	CCWGG	2 cut(s)	369, 1025
BclI	TGATCA	2 cut(s)	460, 990
BcnI	CCSGG	1 cut(s)	66
BcoDI	GTCTC	3 cut(s)	514, 756, 1022
BfaI	CTAG	3 cut(s)	159, 186, 758
BfrI	CTTAAG	1 cut(s)	604
BglII	AGATCT	1 cut(s)	473
BisI	GCNGC	5 cut(s)	197, 240, 243, 300, 884
BlsI	GCNGC	5 cut(s)	198, 241, 244, 301, 885
Bme1390I	CCNGG	3 cut(s)	66, 369, 1025
Bme18I	GGWCC	1 cut(s)	68
BmgT120I	GGNCC	1 cut(s)	68
BmiI	GGNNCC	4 cut(s)	683, 740, 866, 1071
BmrFI	CCNGG	3 cut(s)	66, 369, 1025
BmsI	GCATC	3 cut(s)	193, 787, 970
BoxI	GACNNNNGTC	1 cut(s)	1034
BpmI	CTGGAG	1 cut(s)	505
Bpu14I	TTCGAA	1 cut(s)	644
BpuMI	CCSGG	1 cut(s)	66
BsaHI	GRCGYC	1 cut(s)	376
BsaI	GGTCTC	1 cut(s)	514
BsaJI	CCNNGG	4 cut(s)	64, 368, 709, 955
BsaWI	WCCGGW	1 cut(s)	40
BsaXI	ACNNNNNCTCC	2 cut(s)	41, 71
Bsc4I	CCNNNNNNNGG	3 cut(s)	289, 290, 715
Bse1I	ACTGG	4 cut(s)	709, 740, 884, 1038
Bse3DI	GCAATG	1 cut(s)	993
BseAI	TCCGGA	1 cut(s)	40
BseBI	CCWGG	2 cut(s)	369, 1025
BseDI	CCNNGG	4 cut(s)	64, 368, 709, 955
BseGI	GGATG	4 cut(s)	208, 510, 724, 802
BseLI	CCNNNNNNNGG	3 cut(s)	289, 290, 715
BseMI	GCAATG	1 cut(s)	993
BseMII	CTCAG	1 cut(s)	149
BseNI	ACTGG	4 cut(s)	709, 740, 884, 1038
BseRI	GAGGAG	1 cut(s)	354
BseXI	GCAGC	5 cut(s)	208, 251, 254, 286, 870
BsgI	GTGCAG	1 cut(s)	1032
Bsh1236I	CGCG	1 cut(s)	1040
BshFI	GGCC	4 cut(s)	423, 569, 708, 1023
BshNI	GGYRCC	2 cut(s)	681, 738
BsiSI	CCGG	2 cut(s)	41, 66
BslI	CCNNNNNNNGG	3 cut(s)	289, 290, 715
BsmAI	GTCTC	3 cut(s)	514, 756, 1022
BsnI	GGCC	4 cut(s)	423, 569, 708, 1023
Bso31I	GGTCTC	1 cut(s)	514
Bsp119I	TTCGAA	1 cut(s)	644
Bsp13I	TCCGGA	1 cut(s)	40
Bsp143I	GATC	5 cut(s)	460, 473, 527, 901, 990
Bsp19I	CCATGG	1 cut(s)	709
Bsp68I	TCGCGA	1 cut(s)	1040
BspACI	CCGC	3 cut(s)	60, 172, 199
BspANI	GGCC	4 cut(s)	423, 569, 708, 1023
BspCNI	CTCAG	1 cut(s)	148
BspEI	TCCGGA	1 cut(s)	40
BspFNI	CGCG	1 cut(s)	1040
BspHI	TCATGA	1 cut(s)	457
BspLI	GGNNCC	4 cut(s)	683, 740, 866, 1071
BspPI	GGATC	2 cut(s)	535, 909
BspQI	GCTCTTC	1 cut(s)	684
BspT104I	TTCGAA	1 cut(s)	644
BspT107I	GGYRCC	2 cut(s)	681, 738
BspTI	CTTAAG	1 cut(s)	604
BspTNI	GGTCTC	1 cut(s)	514
BsrDI	GCAATG	1 cut(s)	993
BsrI	ACTGG	4 cut(s)	709, 740, 884, 1038
BssECI	CCNNGG	4 cut(s)	64, 368, 709, 955
BssMI	GATC	5 cut(s)	460, 473, 527, 901, 990
BssNAI	GTATAC	1 cut(s)	177
BssNI	GRCGYC	1 cut(s)	376
BssT1I	CCWWGG	1 cut(s)	709
Bst1107I	GTATAC	1 cut(s)	177
Bst2UI	CCWGG	2 cut(s)	369, 1025
Bst4CI	ACNGT	5 cut(s)	166, 220, 265, 681, 979
Bst6I	CTCTTC	3 cut(s)	684, 719, 1082
BstACI	GRCGYC	1 cut(s)	376
BstAFI	CTTAAG	1 cut(s)	604
BstBI	TTCGAA	1 cut(s)	644
BstDEI	CTNAG	3 cut(s)	135, 801, 897
BstDSI	CCRYGG	2 cut(s)	709, 955
BstF5I	GGATG	4 cut(s)	208, 510, 724, 802
BstFNI	CGCG	1 cut(s)	1040
BstHHI	GCGC	1 cut(s)	696
BstKTI	GATC	5 cut(s)	463, 476, 530, 904, 993
BstMAI	GTCTC	3 cut(s)	514, 756, 1022
BstMBI	GATC	5 cut(s)	460, 473, 527, 901, 990
BstNI	CCWGG	2 cut(s)	369, 1025
BstNSI	RCATGY	2 cut(s)	37, 256
BstPAI	GACNNNNGTC	1 cut(s)	1034
BstSCI	CCNGG	3 cut(s)	64, 367, 1023
BstUI	CGCG	1 cut(s)	1040
BstV1I	GCAGC	5 cut(s)	208, 251, 254, 286, 870
BstX2I	RGATCY	1 cut(s)	473
BstYI	RGATCY	1 cut(s)	473
BstZ17I	GTATAC	1 cut(s)	177
BsuRI	GGCC	4 cut(s)	423, 569, 708, 1023
BtgI	CCRYGG	2 cut(s)	709, 955
BtsCI	GGATG	4 cut(s)	208, 510, 724, 802
BtsIMutI	CAGTG	2 cut(s)	162, 1031
BtuMI	TCGCGA	1 cut(s)	1040
CciI	TCATGA	1 cut(s)	457
CfoI	GCGC	1 cut(s)	696
Cfr13I	GGNCC	1 cut(s)	68
CseI	GACGC	1 cut(s)	365
CviAII	CATG	7 cut(s)	34, 95, 253, 458, 553, 710, 830
DdeI	CTNAG	3 cut(s)	135, 801, 897
DpnI	GATC	5 cut(s)	462, 475, 529, 903, 992
DpnII	GATC	5 cut(s)	460, 473, 527, 901, 990
DrdI	GACNNNNNNGTC	1 cut(s)	782
DseDI	GACNNNNNNGTC	1 cut(s)	782
EaeI	YGGCCR	2 cut(s)	421, 706
Eam1104I	CTCTTC	3 cut(s)	684, 719, 1082
EarI	CTCTTC	3 cut(s)	684, 719, 1082
EciI	GGCGGA	1 cut(s)	49
Eco130I	CCWWGG	1 cut(s)	709
Eco31I	GGTCTC	1 cut(s)	514
Eco47I	GGWCC	1 cut(s)	68
Eco57I	CTGAAG	2 cut(s)	434, 747
EcoRI	GAATTC	1 cut(s)	404
EcoRII	CCWGG	2 cut(s)	367, 1023
EcoT14I	CCWWGG	1 cut(s)	709
ErhI	CCWWGG	1 cut(s)	709
FaeI	CATG	7 cut(s)	37, 98, 256, 461, 556, 713, 833
FalI	AAGNNNNNCTT	4 cut(s)	608, 640, 765, 797
FatI	CATG	7 cut(s)	33, 94, 252, 457, 552, 709, 829
FauI	CCCGC	1 cut(s)	192
FbaI	TGATCA	2 cut(s)	460, 990
FblI	GTMKAC	1 cut(s)	176
Fnu4HI	GCNGC	5 cut(s)	197, 240, 243, 300, 884
FokI	GGATG	4 cut(s)	215, 517, 731, 809
Fsp4HI	GCNGC	5 cut(s)	197, 240, 243, 300, 884
FspBI	CTAG	3 cut(s)	159, 186, 758
GlaI	GCGC	1 cut(s)	695
GluI	GCNGC	5 cut(s)	197, 240, 243, 300, 884
GsuI	CTGGAG	1 cut(s)	505
HaeIII	GGCC	4 cut(s)	423, 569, 708, 1023
HapII	CCGG	2 cut(s)	41, 66
HgaI	GACGC	1 cut(s)	365
HhaI	GCGC	1 cut(s)	696
Hin1I	GRCGYC	1 cut(s)	376
Hin1II	CATG	7 cut(s)	37, 98, 256, 461, 556, 713, 833
Hin6I	GCGC	1 cut(s)	694
HinP1I	GCGC	1 cut(s)	694
HincII	GTYRAC	1 cut(s)	733
HindII	GTYRAC	1 cut(s)	733
HindIII	AAGCTT	2 cut(s)	607, 914
HinfI	GANTC	8 cut(s)	133, 361, 445, 487, 590, 947, 1062, 1134
HpaII	CCGG	2 cut(s)	41, 66
HphI	GGTGA	3 cut(s)	41, 110, 527
Hpy166II	GTNNAC	3 cut(s)	177, 360, 733
Hpy188I	TCNGA	5 cut(s)	138, 473, 966, 1061, 1117
Hpy188III	TCNNGA	5 cut(s)	41, 458, 484, 599, 1039
Hpy8I	GTNNAC	3 cut(s)	177, 360, 733
HpyAV	CCTTC	4 cut(s)	135, 559, 931, 961
HpyCH4III	ACNGT	5 cut(s)	166, 220, 265, 681, 979
HpyCH4V	TGCA	7 cut(s)	113, 226, 302, 313, 677, 850, 1013
HpyF3I	CTNAG	3 cut(s)	135, 801, 897
Hsp92I	GRCGYC	1 cut(s)	376
Hsp92II	CATG	7 cut(s)	37, 98, 256, 461, 556, 713, 833
HspAI	GCGC	1 cut(s)	694
Kpn2I	TCCGGA	1 cut(s)	40
Ksp22I	TGATCA	2 cut(s)	460, 990
Kzo9I	GATC	5 cut(s)	460, 473, 527, 901, 990
LguI	GCTCTTC	1 cut(s)	684
LmnI	GCTCC	1 cut(s)	940
Lsp1109I	GCAGC	5 cut(s)	208, 251, 254, 286, 870
LweI	GCATC	3 cut(s)	193, 787, 970
MaeI	CTAG	3 cut(s)	159, 186, 758
MaeIII	GTNAC	2 cut(s)	833, 973
MalI	GATC	5 cut(s)	462, 475, 529, 903, 992
MboI	GATC	5 cut(s)	460, 473, 527, 901, 990
MboII	GAAGA	5 cut(s)	15, 440, 701, 736, 1069
MfeI	CAATTG	1 cut(s)	246
MflI	RGATCY	1 cut(s)	473
MlsI	TGGCCA	2 cut(s)	423, 708
MluCI	AATT	8 cut(s)	246, 337, 342, 404, 433, 499, 1045, 1095
MluNI	TGGCCA	2 cut(s)	423, 708
MlyI	GAGTC	4 cut(s)	127, 355, 496, 1071
MnlI	CCTC	9 cut(s)	48, 67, 81, 90, 111, 201, 366, 375, 518
Mox20I	TGGCCA	2 cut(s)	423, 708
MroI	TCCGGA	1 cut(s)	40
MroXI	GAANNNNTTC	2 cut(s)	444, 594
MscI	TGGCCA	2 cut(s)	423, 708
MseI	TTAA	8 cut(s)	345, 432, 605, 660, 858, 1049, 1101, 1147
MslI	CAYNNNNRTG	2 cut(s)	99, 167
Msp20I	TGGCCA	2 cut(s)	423, 708
MspA1I	CMGCKG	1 cut(s)	199
MspCI	CTTAAG	1 cut(s)	604
MspI	CCGG	2 cut(s)	41, 66
MspR9I	CCNGG	3 cut(s)	66, 369, 1025
MunI	CAATTG	1 cut(s)	246
MvaI	CCWGG	2 cut(s)	369, 1025
MvnI	CGCG	1 cut(s)	1040
NciI	CCSGG	1 cut(s)	66
NcoI	CCATGG	1 cut(s)	709
NdeII	GATC	5 cut(s)	460, 473, 527, 901, 990
NlaIII	CATG	7 cut(s)	37, 98, 256, 461, 556, 713, 833
NlaIV	GGNNCC	4 cut(s)	683, 740, 866, 1071
NmuCI	GTSAC	2 cut(s)	833, 973
NruI	TCGCGA	1 cut(s)	1040
NspI	RCATGY	2 cut(s)	37, 256
NspV	TTCGAA	1 cut(s)	644
OliI	CACNNNNGTG	1 cut(s)	167
PagI	TCATGA	1 cut(s)	457
PciI	ACATGT	1 cut(s)	252
PciSI	GCTCTTC	1 cut(s)	684
PdmI	GAANNNNTTC	2 cut(s)	444, 594
PfeI	GAWTC	4 cut(s)	445, 590, 947, 1134
PkrI	GCNGC	5 cut(s)	198, 241, 244, 301, 885
PleI	GAGTC	4 cut(s)	127, 355, 495, 1070
PpsI	GAGTC	4 cut(s)	127, 355, 495, 1070
PscI	ACATGT	1 cut(s)	252
PshAI	GACNNNNGTC	1 cut(s)	1034
PshBI	ATTAAT	1 cut(s)	345
Psp6I	CCWGG	2 cut(s)	367, 1023
PspGI	CCWGG	2 cut(s)	367, 1023
PspN4I	GGNNCC	4 cut(s)	683, 740, 866, 1071
PspPI	GGNCC	1 cut(s)	68
PsuI	RGATCY	1 cut(s)	473
RruI	TCGCGA	1 cut(s)	1040
RseI	CAYNNNNRTG	2 cut(s)	99, 167
SapI	GCTCTTC	1 cut(s)	684
SaqAI	TTAA	8 cut(s)	345, 432, 605, 660, 858, 1049, 1101, 1147
SatI	GCNGC	5 cut(s)	197, 240, 243, 300, 884
Sau3AI	GATC	5 cut(s)	460, 473, 527, 901, 990
Sau96I	GGNCC	1 cut(s)	68
SchI	GAGTC	4 cut(s)	127, 355, 496, 1071
ScrFI	CCNGG	3 cut(s)	66, 369, 1025
SetI	ASST	8 cut(s)	232, 611, 763, 769, 783, 918, 945, 1071
SfaNI	GCATC	3 cut(s)	193, 787, 970
SfuI	TTCGAA	1 cut(s)	644
SinI	GGWCC	1 cut(s)	68
SmiMI	CAYNNNNRTG	2 cut(s)	99, 167
SmlI	CTYRAG	1 cut(s)	604
SmoI	CTYRAG	1 cut(s)	604
Sse9I	AATT	8 cut(s)	246, 337, 342, 404, 433, 499, 1045, 1095
SsiI	CCGC	3 cut(s)	60, 172, 199
SspMI	CTAG	3 cut(s)	159, 186, 758
StyD4I	CCNGG	3 cut(s)	64, 367, 1023
StyI	CCWWGG	1 cut(s)	709
TaaI	ACNGT	5 cut(s)	166, 220, 265, 681, 979
TaqI	TCGA	1 cut(s)	644
TasI	AATT	8 cut(s)	246, 337, 342, 404, 433, 499, 1045, 1095
TfiI	GAWTC	4 cut(s)	445, 590, 947, 1134
Tru1I	TTAA	8 cut(s)	345, 432, 605, 660, 858, 1049, 1101, 1147
Tru9I	TTAA	8 cut(s)	345, 432, 605, 660, 858, 1049, 1101, 1147
TscAI	CASTG	2 cut(s)	169, 1038
TseFI	GTSAC	2 cut(s)	833, 973
TseI	GCWGC	5 cut(s)	196, 239, 242, 299, 883
Tsp45I	GTSAC	2 cut(s)	833, 973
TspDTI	ATGAA	6 cut(s)	83, 510, 569, 813, 818, 950
TspGWI	ACGGA	2 cut(s)	323, 944
TspRI	CASTG	2 cut(s)	169, 1038
Vha464I	CTTAAG	1 cut(s)	604
VpaK11BI	GGWCC	1 cut(s)	68
VspI	ATTAAT	1 cut(s)	345
XapI	RAATTY	2 cut(s)	404, 1045
XceI	RCATGY	2 cut(s)	37, 256
XmiI	GTMKAC	1 cut(s)	176
XmnI	GAANNNNTTC	2 cut(s)	444, 594
XspI	CTAG	3 cut(s)	159, 186, 758

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.