Rh3BG286000

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000356
Physical Location & Seq
Forward (+)
28461836 .. 28463014
1179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG286000.1

Sequence Viewer

Length: 1179 bp
ATGGCCCCAGCCCAGACCACAGTTGATTACACCGGCTCTGTCGTCCGATACACACAGAATCGAAAGTGGTCCTCCTCAGATCCGTTGCCGGCGGCTGCCTGTTCTTCGATTAACAACCCATTTGCTGCCGAGTTTGTGCACTCTTGTACAAATTACAGAGCCCGTGCTTCTTCGATCAATCGAGTTTTATGGACACCTTCTGGGAGGCGTCTTATTACAGGCTCTCAAAACGGGGAGTTCACTCTCTGGAATGGTGAGTCATTCAAACACGAATTGAGGATTCAGGCTCATGATCAACCAATCAGGTCCATGGCGTGGAGTTTTGATGATGAAGATTGGATCTCTGGTGATGATGGAGGCACAATCAGGTATTGGAAGAGTAACATGAGTAATGTGCTTGTCATTGAATCTGCTCACCATGAATCGGTTCGGGACTTGAGCTTCTGTCGGAGTAATTTGAAGTTTTGTTCATGTTCGGATGACACTAGTGTTAAAATCTGGGATTTTGAACGGTGCCAACAGGAGCAGACATTGACCGGCCATGGTTGGAATGTGACGAGTGTTGACTGGCACCCCACAAACTCTCTAATAGCTTCGGGTGGGAAAGACAGTGTCGTCAAACTGTGGGATGCTAGGTCACGGAGAGAACTCTGTTCGTTTTATGATCACAAAAATTGGGTGCATTCTGTCAAGTGGAACCGAAATGGTAACTGGCTGCTAACTGCTTCCAAGGATCATGTCATTAAGCTTTACGACTTGAGGGCTATGAAGGAACTTAAATCTTTCCGCGGCCATCAGAACCAAGTGACTGCTCTAGCTTGGCATCCTCTTACTGAAGACTATTTTGTCAGTGGGAGTAGTGATGGATCCATTTTCCATTGGCTTGTTGGCCATGTAACTCCCCAGGTTCAAATTCCTAACGCACACACTAACAGTTACAATAATAATAGTGTGTGGGATCTCCAGTGGCATCCTATTGGTCATATGATTTGTAGTGGTAGCAATGATCGCACCACCAAGTTTTGGTGCAGAACAGCAGATAAATCTAAATTCAGTCAGAATCAAAGTAATTTTCCCTTTCCATTTCATGGACCACCAACTATTGCTACTCGAGACCAAGGAACCCTTGGTTCAGGAGGTGTTGGAACCATTATACCCGGTGTTGGAATTGCGGTTTAA

Protein Analysis

392

Amino Acids

44.25

Weight (kDa)

8.07

Isoelectric Point (pI)

34.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 17 - 214 4.2e-23 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 50 - 213 1.7e-27 THOC3 beta-propeller domain
WDR55 PF24796 50 - 126 4.3e-06 WDR55
Beta-prop_WDR3_1st PF25173 53 - 203 2.9e-21 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 53 - 262 1.4e-19 G protein beta WD-40 repeat protein
Beta-prop_IFT140_1st PF23383 54 - 134 4e-09 IFT140 first beta-propeller
Beta-prop_WDR5 PF25175 56 - 148 7.6e-09 WDR5 beta-propeller domain
Beta-prop_EIPR1 PF23609 58 - 209 9.8e-08 EIPR1 beta-propeller
WD40_Prp19 PF24814 59 - 262 5.5e-32 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 59 - 217 1.4e-16 WDHD1 first WD40 domain
EIF3I PF24805 89 - 176 2.3e-07 EIF3I
WD40_MABP1-WDR62_2nd PF24782 101 - 269 5.1e-12 MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd PF25047 103 - 273 1.2e-16 TEP-1 second beta-propeller
Beta-prop_Aladin PF25460 127 - 258 1.3e-07 Aladin seven-bladed propeller
WDR55 PF24796 133 - 257 3.4e-16 WDR55
Beta-prop_CAF1B_HIR1 PF24105 135 - 215 2e-09 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 136 - 298 7.1e-19 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 137 - 211 2.3e-10 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 138 - 252 5.1e-26 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 151 - 299 2.4e-21 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 155 - 241 1.2e-07 WDR90/POC16, second beta-propeller
Beta-prop_IFT140_1st PF23383 161 - 248 3.7e-06 IFT140 first beta-propeller
WD40 PF00400 173 - 210 1.1e-10 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 177 - 257 4.4e-09 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML PF23409 179 - 284 1e-08 Echinoderm microtubule-associated protein first beta-propeller
EIF3I PF24805 183 - 264 4.3e-10 EIF3I
Beta-prop_WDR3_1st PF25173 184 - 342 4.3e-25 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 188 - 346 2.7e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 200 - 343 4.2e-08 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 205 - 347 2.6e-20 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 217 - 347 4.1e-11 WDR3 second beta-propeller domain
WD40 PF00400 217 - 252 8.2e-08 WD domain, G-beta repeat
Beta-prop_DCAF4 PF23761 218 - 280 2.5e-06 DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_WDR5 PF25175 220 - 342 1.7e-17 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 223 - 353 7.9e-24 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 226 - 338 3.3e-06 Aladin seven-bladed propeller
WD40_WDHD1_1st PF24817 227 - 336 4.5e-13 WDHD1 first WD40 domain
WD40 PF00400 257 - 294 8.9e-06 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 259 - 359 3.8e-06 CAF1B/HIR1 beta-propeller domain
WD40_Prp19 PF24814 259 - 342 7.1e-09 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica Prupe.2G034300_v2.0.a1
pyrus_communis pycom02g24420 pycom07g02650
rosa_chinensis RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 614, 845
AccB1I GGYRCC 2 cut(s) 513, 570
AccB7I CCANNNNNTGG 3 cut(s) 315, 1023, 1088
AccII CGCG 1 cut(s) 789
AciI CCGC 4 cut(s) 92, 787, 789, 1172
AclWI GGATC 6 cut(s) 74, 347, 741, 861, 874, 966
AcoI YGGCCR 3 cut(s) 538, 790, 889
AcsI RAATTY 2 cut(s) 912, 1049
AcuI CTGAAG 1 cut(s) 855
AcyI GRCGYC 1 cut(s) 208
AfaI GTAC 1 cut(s) 148
AfiI CCNNNNNNNGG 5 cut(s) 315, 424, 1023, 1088, 1163
AgsI TTSAA 5 cut(s) 265, 407, 460, 509, 911
AhlI ACTAGT 1 cut(s) 485
AjnI CCWGG 1 cut(s) 903
AluBI AGCT 4 cut(s) 441, 593, 748, 818
AluI AGCT 4 cut(s) 441, 593, 748, 818
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 1107
Alw44I GTGCAC 1 cut(s) 137
AlwI GGATC 6 cut(s) 74, 347, 741, 861, 874, 966
Ama87I CYCGRG 1 cut(s) 1110
AoxI GGCC 4 cut(s) 3, 538, 790, 889
ApaLI GTGCAC 1 cut(s) 137
ApeKI GCWGC 3 cut(s) 95, 125, 715
ApoI RAATTY 2 cut(s) 912, 1049
Asp700I GAANNNNTTC 1 cut(s) 426
AspS9I GGNCC 4 cut(s) 4, 69, 306, 1091
AsuC2I CCSGG 1 cut(s) 1158
AsuHPI GGTGA 3 cut(s) 266, 359, 407
AvaI CYCGRG 1 cut(s) 1110
AvaII GGWCC 3 cut(s) 69, 306, 1091
BaeGI GKGCMC 1 cut(s) 141
BalI TGGCCA 1 cut(s) 891
BamHI GGATCC 1 cut(s) 866
BanI GGYRCC 2 cut(s) 513, 570
BanII GRGCYC 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 843
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 3 cut(s) 82, 112, 702
BccI CCATC 3 cut(s) 347, 801, 857
BciT130I CCWGG 1 cut(s) 905
BclI TGATCA 2 cut(s) 292, 664
BcnI CCSGG 1 cut(s) 1158
BcoDI GTCTC 1 cut(s) 1107
BcuI ACTAGT 1 cut(s) 485
BfaI CTAG 3 cut(s) 486, 633, 815
BisI GCNGC 5 cut(s) 93, 96, 126, 716, 790
BlsI GCNGC 5 cut(s) 94, 97, 127, 717, 791
Bme1390I CCNGG 2 cut(s) 905, 1158
Bme18I GGWCC 3 cut(s) 69, 306, 1091
BmeT110I CYCGRG 1 cut(s) 1110
BmgT120I GGNCC 4 cut(s) 4, 69, 306, 1091
BmiI GGNNCC 7 cut(s) 6, 515, 572, 698, 868, 1123, 1147
BmrFI CCNGG 2 cut(s) 905, 1158
BmsI GCATC 3 cut(s) 619, 832, 979
BpiI GAAGAC 1 cut(s) 843
BpmI CTGGAG 1 cut(s) 947
BpuEI CTTGAG 2 cut(s) 457, 778
BpuMI CCSGG 1 cut(s) 1158
BsaHI GRCGYC 1 cut(s) 208
BsaI GGTCTC 1 cut(s) 1107
BsaJI CCNNGG 7 cut(s) 309, 541, 729, 787, 903, 1117, 1126
Bsc4I CCNNNNNNNGG 5 cut(s) 315, 424, 1023, 1088, 1163
Bse118I RCCGGY 3 cut(s) 32, 88, 536
Bse1I ACTGG 3 cut(s) 572, 716, 964
Bse3DI GCAATG 1 cut(s) 1009
BseBI CCWGG 1 cut(s) 905
BseDI CCNNGG 7 cut(s) 309, 541, 729, 787, 903, 1117, 1126
BseGI GGATG 4 cut(s) 484, 634, 823, 970
BseLI CCNNNNNNNGG 5 cut(s) 315, 424, 1023, 1088, 1163
BseMI GCAATG 1 cut(s) 1009
BseMII CTCAG 1 cut(s) 90
BseNI ACTGG 3 cut(s) 572, 716, 964
BseRI GAGGAG 1 cut(s) 64
BseSI GKGCMC 1 cut(s) 141
BseXI GCAGC 3 cut(s) 82, 112, 702
BseYI CCCAGC 1 cut(s) 7
BsgI GTGCAG 1 cut(s) 1048
Bsh1236I CGCG 1 cut(s) 789
BshFI GGCC 4 cut(s) 5, 540, 792, 891
BshNI GGYRCC 2 cut(s) 513, 570
BsiHKAI GWGCWC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 1110
BsiSI CCGG 4 cut(s) 33, 89, 537, 1158
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 5 cut(s) 315, 424, 1023, 1088, 1163
BsmAI GTCTC 1 cut(s) 1107
BsmFI GGGAC 1 cut(s) 446
BsmI GAATGC 1 cut(s) 682
BsnI GGCC 4 cut(s) 5, 540, 792, 891
Bso31I GGTCTC 1 cut(s) 1107
BsoBI CYCGRG 1 cut(s) 1110
Bsp1286I GDGCHC 2 cut(s) 141, 163
Bsp1407I TGTACA 1 cut(s) 146
Bsp143I GATC 9 cut(s) 79, 174, 292, 339, 664, 733, 866, 958, 1006
Bsp19I CCATGG 2 cut(s) 309, 541
BspACI CCGC 4 cut(s) 92, 787, 789, 1172
BspANI GGCC 4 cut(s) 5, 540, 792, 891
BspCNI CTCAG 1 cut(s) 89
BspFNI CGCG 1 cut(s) 789
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 7 cut(s) 6, 515, 572, 698, 868, 1123, 1147
BspPI GGATC 6 cut(s) 74, 347, 741, 861, 874, 966
BspT107I GGYRCC 2 cut(s) 513, 570
BspTNI GGTCTC 1 cut(s) 1107
BsrDI GCAATG 1 cut(s) 1009
BsrFI RCCGGY 3 cut(s) 32, 88, 536
BsrGI TGTACA 1 cut(s) 146
BsrI ACTGG 3 cut(s) 572, 716, 964
BssAI RCCGGY 3 cut(s) 32, 88, 536
BssECI CCNNGG 7 cut(s) 309, 541, 729, 787, 903, 1117, 1126
BssMI GATC 9 cut(s) 79, 174, 292, 339, 664, 733, 866, 958, 1006
BssNI GRCGYC 1 cut(s) 208
BssT1I CCWWGG 5 cut(s) 309, 541, 729, 1117, 1126
Bst2UI CCWGG 1 cut(s) 905
Bst4CI ACNGT 5 cut(s) 22, 513, 611, 624, 935
Bst6I CTCTTC 1 cut(s) 371
BstACI GRCGYC 1 cut(s) 208
BstAUI TGTACA 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 90
BstDEI CTNAG 1 cut(s) 76
BstDSI CCRYGG 3 cut(s) 309, 541, 787
BstF5I GGATG 4 cut(s) 484, 634, 823, 970
BstFNI CGCG 1 cut(s) 789
BstKTI GATC 9 cut(s) 82, 177, 295, 342, 667, 736, 869, 961, 1009
BstMAI GTCTC 1 cut(s) 1107
BstMBI GATC 9 cut(s) 79, 174, 292, 339, 664, 733, 866, 958, 1006
BstMWI GCNNNNNNNGC 1 cut(s) 1008
BstNI CCWGG 1 cut(s) 905
BstSCI CCNGG 2 cut(s) 903, 1156
BstSLI GKGCMC 1 cut(s) 141
BstUI CGCG 1 cut(s) 789
BstV1I GCAGC 3 cut(s) 82, 112, 702
BstV2I GAAGAC 1 cut(s) 843
BstX2I RGATCY 4 cut(s) 79, 339, 866, 958
BstYI RGATCY 4 cut(s) 79, 339, 866, 958
BsuRI GGCC 4 cut(s) 5, 540, 792, 891
BtgI CCRYGG 3 cut(s) 309, 541, 787
BtsCI GGATG 4 cut(s) 484, 634, 823, 970
BtsIMutI CAGTG 3 cut(s) 616, 856, 971
Cac8I GCNNGC 1 cut(s) 90
CciI TCATGA 1 cut(s) 289
Cfr10I RCCGGY 3 cut(s) 32, 88, 536
Cfr13I GGNCC 4 cut(s) 4, 69, 306, 1091
Cfr42I CCGCGG 1 cut(s) 790
CseI GACGC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 147
CviAII CATG 9 cut(s) 290, 310, 385, 419, 471, 542, 737, 893, 1088
CviQI GTAC 1 cut(s) 147
DdeI CTNAG 1 cut(s) 76
DpnI GATC 9 cut(s) 81, 176, 294, 341, 666, 735, 868, 960, 1008
DpnII GATC 9 cut(s) 79, 174, 292, 339, 664, 733, 866, 958, 1006
DrdI GACNNNNNNGTC 2 cut(s) 614, 845
DseDI GACNNNNNNGTC 2 cut(s) 614, 845
EaeI YGGCCR 3 cut(s) 538, 790, 889
Eam1104I CTCTTC 1 cut(s) 371
EarI CTCTTC 1 cut(s) 371
Eco130I CCWWGG 5 cut(s) 309, 541, 729, 1117, 1126
Eco24I GRGCYC 1 cut(s) 163
Eco31I GGTCTC 1 cut(s) 1107
Eco47I GGWCC 3 cut(s) 69, 306, 1091
Eco57I CTGAAG 1 cut(s) 855
Eco88I CYCGRG 1 cut(s) 1110
EcoRII CCWGG 1 cut(s) 903
EcoT14I CCWWGG 5 cut(s) 309, 541, 729, 1117, 1126
EcoT38I GRGCYC 1 cut(s) 163
ErhI CCWWGG 5 cut(s) 309, 541, 729, 1117, 1126
FaeI CATG 9 cut(s) 293, 313, 388, 422, 474, 545, 740, 896, 1091
FalI AAGNNNNNCTT 2 cut(s) 1110, 1142
FaqI GGGAC 1 cut(s) 446
FatI CATG 9 cut(s) 289, 309, 384, 418, 470, 541, 736, 892, 1087
FauNDI CATATG 1 cut(s) 984
FbaI TGATCA 2 cut(s) 292, 664
Fnu4HI GCNGC 5 cut(s) 93, 96, 126, 716, 790
FokI GGATG 4 cut(s) 491, 641, 810, 957
FriOI GRGCYC 1 cut(s) 163
Fsp4HI GCNGC 5 cut(s) 93, 96, 126, 716, 790
FspBI CTAG 3 cut(s) 486, 633, 815
GluI GCNGC 5 cut(s) 93, 96, 126, 716, 790
GsaI CCCAGC 1 cut(s) 11
GsuI CTGGAG 1 cut(s) 947
HaeIII GGCC 4 cut(s) 5, 540, 792, 891
HapII CCGG 4 cut(s) 33, 89, 537, 1158
HgaI GACGC 1 cut(s) 197
Hin1I GRCGYC 1 cut(s) 208
Hin1II CATG 9 cut(s) 293, 313, 388, 422, 474, 545, 740, 896, 1091
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 1 cut(s) 746
HinfI GANTC 6 cut(s) 58, 257, 280, 407, 422, 1060
HpaII CCGG 4 cut(s) 33, 89, 537, 1158
HphI GGTGA 3 cut(s) 266, 359, 407
Hpy166II GTNNAC 3 cut(s) 139, 240, 565
Hpy188I TCNGA 6 cut(s) 47, 79, 450, 478, 798, 1059
Hpy188III TCNNGA 5 cut(s) 247, 290, 431, 1112, 1134
Hpy8I GTNNAC 3 cut(s) 139, 240, 565
HpyAV CCTTC 2 cut(s) 207, 763
HpyCH4III ACNGT 5 cut(s) 22, 513, 611, 624, 935
HpyCH4V TGCA 3 cut(s) 139, 682, 1029
HpyF10VI GCNNNNNNNGC 1 cut(s) 1008
HpyF3I CTNAG 1 cut(s) 76
Hsp92I GRCGYC 1 cut(s) 208
Hsp92II CATG 9 cut(s) 293, 313, 388, 422, 474, 545, 740, 896, 1091
KroI GCCGGC 1 cut(s) 88
KroNI GCCGGC 1 cut(s) 90
Ksp22I TGATCA 2 cut(s) 292, 664
KspI CCGCGG 1 cut(s) 790
Kzo9I GATC 9 cut(s) 79, 174, 292, 339, 664, 733, 866, 958, 1006
LmnI GCTCC 1 cut(s) 523
Lsp1109I GCAGC 3 cut(s) 82, 112, 702
LweI GCATC 3 cut(s) 619, 832, 979
MaeI CTAG 3 cut(s) 486, 633, 815
MaeIII GTNAC 7 cut(s) 380, 553, 636, 707, 805, 895, 935
MalI GATC 9 cut(s) 81, 176, 294, 341, 666, 735, 868, 960, 1008
MboI GATC 9 cut(s) 79, 174, 292, 339, 664, 733, 866, 958, 1006
MboII GAAGA 5 cut(s) 96, 162, 344, 388, 848
MflI RGATCY 4 cut(s) 79, 339, 866, 958
MhlI GDGCHC 2 cut(s) 141, 163
MlsI TGGCCA 1 cut(s) 891
MluCI AATT 8 cut(s) 151, 272, 454, 673, 912, 1049, 1069, 1167
MluNI TGGCCA 1 cut(s) 891
MlyI GAGTC 1 cut(s) 266
MmeI TCCRAC 4 cut(s) 428, 527, 1123, 1144
MnlI CCTC 8 cut(s) 82, 85, 198, 270, 350, 753, 837, 1130
Mox20I TGGCCA 1 cut(s) 891
MroNI GCCGGC 1 cut(s) 88
MroXI GAANNNNTTC 1 cut(s) 426
MscI TGGCCA 1 cut(s) 891
MseI TTAA 5 cut(s) 111, 492, 744, 777, 1177
Msp20I TGGCCA 1 cut(s) 891
MspA1I CMGCKG 1 cut(s) 789
MspI CCGG 4 cut(s) 33, 89, 537, 1158
MspR9I CCNGG 2 cut(s) 905, 1158
Mva1269I GAATGC 1 cut(s) 682
MvaI CCWGG 1 cut(s) 905
MvnI CGCG 1 cut(s) 789
MwoI GCNNNNNNNGC 1 cut(s) 1008
NaeI GCCGGC 1 cut(s) 90
NciI CCSGG 1 cut(s) 1158
NcoI CCATGG 2 cut(s) 309, 541
NdeI CATATG 1 cut(s) 984
NdeII GATC 9 cut(s) 79, 174, 292, 339, 664, 733, 866, 958, 1006
NgoMIV GCCGGC 1 cut(s) 88
NlaIII CATG 9 cut(s) 293, 313, 388, 422, 474, 545, 740, 896, 1091
NlaIV GGNNCC 7 cut(s) 6, 515, 572, 698, 868, 1123, 1147
NmeAIII GCCGAG 1 cut(s) 154
NmuCI GTSAC 3 cut(s) 553, 636, 805
PaeR7I CTCGAG 1 cut(s) 1110
PagI TCATGA 1 cut(s) 289
PctI GAATGC 1 cut(s) 682
PdiI GCCGGC 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 426
PfeI GAWTC 5 cut(s) 58, 280, 407, 422, 1060
PflFI GACNNNGTC 1 cut(s) 611
PflMI CCANNNNNTGG 3 cut(s) 315, 1023, 1088
PkrI GCNGC 5 cut(s) 94, 97, 127, 717, 791
PleI GAGTC 1 cut(s) 265
PpsI GAGTC 1 cut(s) 265
Psp6I CCWGG 1 cut(s) 903
PspFI CCCAGC 1 cut(s) 7
PspGI CCWGG 1 cut(s) 903
PspN4I GGNNCC 7 cut(s) 6, 515, 572, 698, 868, 1123, 1147
PspPI GGNCC 4 cut(s) 4, 69, 306, 1091
PsuI RGATCY 4 cut(s) 79, 339, 866, 958
PsyI GACNNNGTC 1 cut(s) 611
RsaI GTAC 1 cut(s) 148
RsaNI GTAC 1 cut(s) 147
SacII CCGCGG 1 cut(s) 790
SaqAI TTAA 5 cut(s) 111, 492, 744, 777, 1177
SatI GCNGC 5 cut(s) 93, 96, 126, 716, 790
Sau3AI GATC 9 cut(s) 79, 174, 292, 339, 664, 733, 866, 958, 1006
Sau96I GGNCC 4 cut(s) 4, 69, 306, 1091
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 2 cut(s) 905, 1158
SduI GDGCHC 2 cut(s) 141, 163
SfaNI GCATC 3 cut(s) 619, 832, 979
Sfr274I CTCGAG 1 cut(s) 1110
Sfr303I CCGCGG 1 cut(s) 790
SgrBI CCGCGG 1 cut(s) 790
SinI GGWCC 3 cut(s) 69, 306, 1091
SlaI CTCGAG 1 cut(s) 1110
SmlI CTYRAG 3 cut(s) 436, 757, 1110
SmoI CTYRAG 3 cut(s) 436, 757, 1110
SpeI ACTAGT 1 cut(s) 485
Sse9I AATT 8 cut(s) 151, 272, 454, 673, 912, 1049, 1069, 1167
SsiI CCGC 4 cut(s) 92, 787, 789, 1172
SspMI CTAG 3 cut(s) 486, 633, 815
StyD4I CCNGG 2 cut(s) 903, 1156
StyI CCWWGG 5 cut(s) 309, 541, 729, 1117, 1126
TaaI ACNGT 5 cut(s) 22, 513, 611, 624, 935
TaqI TCGA 5 cut(s) 61, 107, 173, 181, 1111
TasI AATT 8 cut(s) 151, 272, 454, 673, 912, 1049, 1069, 1167
TatI WGTACW 1 cut(s) 146
TauI GCSGC 2 cut(s) 95, 792
TfiI GAWTC 5 cut(s) 58, 280, 407, 422, 1060
Tru1I TTAA 5 cut(s) 111, 492, 744, 777, 1177
Tru9I TTAA 5 cut(s) 111, 492, 744, 777, 1177
TscAI CASTG 3 cut(s) 616, 856, 971
TseFI GTSAC 3 cut(s) 553, 636, 805
TseI GCWGC 3 cut(s) 95, 125, 715
Tsp45I GTSAC 3 cut(s) 553, 636, 805
TspDTI ATGAA 5 cut(s) 345, 435, 459, 782, 1076
TspGWI ACGGA 2 cut(s) 72, 655
TspRI CASTG 3 cut(s) 616, 856, 971
Tth111I GACNNNGTC 1 cut(s) 611
Van91I CCANNNNNTGG 3 cut(s) 315, 1023, 1088
VneI GTGCAC 1 cut(s) 137
VpaK11BI GGWCC 3 cut(s) 69, 306, 1091
XapI RAATTY 2 cut(s) 912, 1049
XcmI CCANNNNNNNNNTGG 2 cut(s) 884, 1124
XhoI CTCGAG 1 cut(s) 1110
XmnI GAANNNNTTC 1 cut(s) 426
XspI CTAG 3 cut(s) 486, 633, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.