Rh5CG564300

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000356
Physical Location & Seq
Reverse (-)
79582227 .. 79597741
15515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG564300.1

Sequence Viewer

Length: 684 bp
ATGGAGGGGAAAACGATTATTGCGGTAATTGCTGCGGGGGTCAGTTACATGGCGATTAATAAGTGGATATGGACTCCAATGAAGCGGGGTCCAAATCCAATACGGAAGGATGATAATGATCCAGCATCTCTTCCTTCATCAGCAGCTGAAGCTTCATCATCATCACCTCCTCCTCCTGAGCAGAAGGATGATCCAGCATCTCTTCCTTCATCATCAGCTGAAGCATCATCATCATCACCACCTCCTCCTCCTCCTGGGCTGGAGTATGATGTGTTTTTGAGTTTCAGGGGTGCTGACACTCGCAAGGGTATCACATCTGATCTATACCATCAACTGCAGAGGAGTGGAATTAAAACATTCATGGATGATCCAGATCTTGAAGTTGGGGATGCTATTTCTCCCACTCTCATTGCAGCAATTGAACAATCACGCATGATTCTCTCTCAATCCATATCTGGTAAGATCATCGGCCACCTTCGCCGGAGAAAGTTTCACGGCGACATGGAAAACTATTCTTCTCGAACGACGACTTATTCAACCTTGGAGGCGAAGCAGAAGACCCATGGCTCTACACTGTTCGACATCGTCCGATTTGGACAGGACGATTTGCGGAACGGCGATAGGGATGTATCGACGTCGCTCCCAGCCTCGTCGTCGAAGCTAAGTCATCGTTGCTGCTGCTGA

Protein Analysis

227

Amino Acids

24.62

Weight (kDa)

6.52

Isoelectric Point (pI)

62.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 89 - 146 3.2e-15 TIR domain
TIR_2 PF13676 91 - 147 5.5e-06 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica Prupe.2G034300_v2.0.a1
pyrus_communis pycom02g24420 pycom07g02650
rosa_chinensis RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 638
AciI CCGC 4 cut(s) 23, 35, 85, 610
AclWI GGATC 3 cut(s) 113, 185, 362
AcoI YGGCCR 1 cut(s) 469
AcuI CTGAAG 2 cut(s) 168, 240
AcyI GRCGYC 1 cut(s) 635
AfiI CCNNNNNNNGG 1 cut(s) 254
AgsI TTSAA 3 cut(s) 380, 422, 537
AjnI CCWGG 1 cut(s) 253
AluBI AGCT 4 cut(s) 146, 152, 218, 661
AluI AGCT 4 cut(s) 146, 152, 218, 661
AlwI GGATC 3 cut(s) 113, 185, 362
AlwNI CAGNNNCTG 1 cut(s) 146
AoxI GGCC 1 cut(s) 469
ApeKI GCWGC 5 cut(s) 32, 143, 413, 675, 678
AseI ATTAAT 1 cut(s) 57
AspS9I GGNCC 1 cut(s) 89
AsuHPI GGTGA 2 cut(s) 156, 228
AvaII GGWCC 1 cut(s) 89
BbsI GAAGAC 1 cut(s) 563
BbvI GCAGC 5 cut(s) 19, 155, 425, 662, 665
BccI CCATC 1 cut(s) 336
BceAI ACGGC 2 cut(s) 511, 631
BciT130I CCWGG 1 cut(s) 255
BfmI CTRYAG 1 cut(s) 335
BglII AGATCT 1 cut(s) 373
BisI GCNGC 5 cut(s) 33, 144, 414, 676, 679
BlsI GCNGC 5 cut(s) 34, 145, 415, 677, 680
Bme1390I CCNGG 1 cut(s) 255
Bme18I GGWCC 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 1 cut(s) 90
BmrFI CCNGG 1 cut(s) 255
BmsI GCATC 4 cut(s) 134, 206, 233, 379
BpiI GAAGAC 1 cut(s) 563
BpmI CTGGAG 1 cut(s) 281
Bpu10I CCTNAGC 1 cut(s) 177
BsaBI GATNNNNATC 2 cut(s) 117, 372
BsaHI GRCGYC 1 cut(s) 635
BsaJI CCNNGG 3 cut(s) 254, 540, 562
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse3DI GCAATG 1 cut(s) 408
Bse8I GATNNNNATC 2 cut(s) 117, 372
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 3 cut(s) 254, 540, 562
BseGI GGATG 5 cut(s) 115, 193, 370, 394, 631
BseJI GATNNNNATC 2 cut(s) 117, 372
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 1 cut(s) 168
BseRI GAGGAG 6 cut(s) 159, 162, 234, 237, 240, 355
BseXI GCAGC 5 cut(s) 19, 155, 425, 662, 665
BseYI CCCAGC 1 cut(s) 643
BshFI GGCC 1 cut(s) 471
BsiSI CCGG 1 cut(s) 481
BslI CCNNNNNNNGG 1 cut(s) 254
BsnI GGCC 1 cut(s) 471
Bsp143I GATC 6 cut(s) 118, 190, 319, 367, 373, 462
Bsp19I CCATGG 1 cut(s) 562
BspACI CCGC 4 cut(s) 23, 35, 85, 610
BspANI GGCC 1 cut(s) 471
BspCNI CTCAG 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 90
BspMAI CTGCAG 1 cut(s) 339
BspPI GGATC 3 cut(s) 113, 185, 362
BsrDI GCAATG 1 cut(s) 408
BssECI CCNNGG 3 cut(s) 254, 540, 562
BssMI GATC 6 cut(s) 118, 190, 319, 367, 373, 462
BssNI GRCGYC 1 cut(s) 635
BssT1I CCWWGG 2 cut(s) 540, 562
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 1 cut(s) 576
Bst6I CTCTTC 2 cut(s) 135, 207
BstACI GRCGYC 1 cut(s) 635
BstDEI CTNAG 2 cut(s) 177, 662
BstDSI CCRYGG 1 cut(s) 562
BstF5I GGATG 5 cut(s) 115, 193, 370, 394, 631
BstKTI GATC 6 cut(s) 121, 193, 322, 370, 376, 465
BstMBI GATC 6 cut(s) 118, 190, 319, 367, 373, 462
BstMWI GCNNNNNNNGC 3 cut(s) 29, 149, 477
BstNI CCWGG 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 253
BstSFI CTRYAG 1 cut(s) 335
BstV1I GCAGC 5 cut(s) 19, 155, 425, 662, 665
BstV2I GAAGAC 1 cut(s) 563
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
BsuRI GGCC 1 cut(s) 471
BtgI CCRYGG 1 cut(s) 562
BtsCI GGATG 5 cut(s) 115, 193, 370, 394, 631
BtsIMutI CAGTG 1 cut(s) 572
CaiI CAGNNNCTG 1 cut(s) 146
Cfr13I GGNCC 1 cut(s) 89
CspCI CAANNNNNGTGG 2 cut(s) 391, 426
CviAII CATG 5 cut(s) 49, 361, 433, 502, 563
CviJI RGCY 8 cut(s) 146, 152, 218, 259, 471, 567, 647, 661
CviKI_1 RGCY 8 cut(s) 146, 152, 218, 259, 471, 567, 647, 661
DdeI CTNAG 2 cut(s) 177, 662
DpnI GATC 6 cut(s) 120, 192, 321, 369, 375, 464
DpnII GATC 6 cut(s) 118, 190, 319, 367, 373, 462
EaeI YGGCCR 1 cut(s) 469
Eam1104I CTCTTC 2 cut(s) 135, 207
EarI CTCTTC 2 cut(s) 135, 207
Eco130I CCWWGG 2 cut(s) 540, 562
Eco47I GGWCC 1 cut(s) 89
Eco57I CTGAAG 2 cut(s) 168, 240
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 2 cut(s) 540, 562
ErhI CCWWGG 2 cut(s) 540, 562
FaeI CATG 5 cut(s) 52, 364, 436, 505, 566
FaiI YATR 9 cut(s) 50, 70, 267, 325, 362, 434, 452, 503, 564
FatI CATG 5 cut(s) 48, 360, 432, 501, 562
FauI CCCGC 2 cut(s) 28, 78
Fnu4HI GCNGC 5 cut(s) 33, 144, 414, 676, 679
FokI GGATG 5 cut(s) 122, 200, 377, 401, 638
Fsp4HI GCNGC 5 cut(s) 33, 144, 414, 676, 679
GluI GCNGC 5 cut(s) 33, 144, 414, 676, 679
GsaI CCCAGC 1 cut(s) 647
GsuI CTGGAG 1 cut(s) 281
HaeIII GGCC 1 cut(s) 471
HapII CCGG 1 cut(s) 481
Hin1I GRCGYC 1 cut(s) 635
Hin1II CATG 5 cut(s) 52, 364, 436, 505, 566
HindIII AAGCTT 1 cut(s) 150
HinfI GANTC 2 cut(s) 73, 436
HpaII CCGG 1 cut(s) 481
HphI GGTGA 2 cut(s) 156, 228
Hpy188I TCNGA 2 cut(s) 319, 590
Hpy188III TCNNGA 4 cut(s) 176, 371, 377, 519
Hpy99I CGWCG 5 cut(s) 529, 637, 640, 655, 658
HpyAV CCTTC 5 cut(s) 100, 144, 178, 216, 485
HpyCH4III ACNGT 1 cut(s) 576
HpyCH4IV ACGT 1 cut(s) 635
HpyCH4V TGCA 2 cut(s) 337, 413
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 149, 477
HpyF3I CTNAG 2 cut(s) 177, 662
HpySE526I ACGT 1 cut(s) 635
Hsp92I GRCGYC 1 cut(s) 635
Hsp92II CATG 5 cut(s) 52, 364, 436, 505, 566
Kzo9I GATC 6 cut(s) 118, 190, 319, 367, 373, 462
LmnI GCTCC 1 cut(s) 645
Lsp1109I GCAGC 5 cut(s) 19, 155, 425, 662, 665
LweI GCATC 4 cut(s) 134, 206, 233, 379
MaeII ACGT 1 cut(s) 635
MaeIII GTNAC 1 cut(s) 44
MalI GATC 6 cut(s) 120, 192, 321, 369, 375, 464
MboI GATC 6 cut(s) 118, 190, 319, 367, 373, 462
MboII GAAGA 4 cut(s) 122, 194, 507, 568
MfeI CAATTG 1 cut(s) 417
MflI RGATCY 1 cut(s) 373
MluCI AATT 3 cut(s) 27, 348, 417
MlyI GAGTC 1 cut(s) 67
MseI TTAA 2 cut(s) 57, 351
MspA1I CMGCKG 2 cut(s) 146, 218
MspI CCGG 1 cut(s) 481
MspR9I CCNGG 1 cut(s) 255
MunI CAATTG 1 cut(s) 417
MvaI CCWGG 1 cut(s) 255
MwoI GCNNNNNNNGC 3 cut(s) 29, 149, 477
NcoI CCATGG 1 cut(s) 562
NdeII GATC 6 cut(s) 118, 190, 319, 367, 373, 462
NlaIII CATG 5 cut(s) 52, 364, 436, 505, 566
NlaIV GGNNCC 1 cut(s) 90
PfeI GAWTC 1 cut(s) 436
PflFI GACNNNGTC 1 cut(s) 584
PkrI GCNGC 5 cut(s) 34, 145, 415, 677, 680
PleI GAGTC 1 cut(s) 67
PpsI GAGTC 1 cut(s) 67
PshBI ATTAAT 1 cut(s) 57
Psp6I CCWGG 1 cut(s) 253
PspFI CCCAGC 1 cut(s) 643
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 90
PspPI GGNCC 1 cut(s) 89
PstI CTGCAG 1 cut(s) 339
PstNI CAGNNNCTG 1 cut(s) 146
PsuI RGATCY 1 cut(s) 373
PsyI GACNNNGTC 1 cut(s) 584
PvuII CAGCTG 2 cut(s) 146, 218
SaqAI TTAA 2 cut(s) 57, 351
SatI GCNGC 5 cut(s) 33, 144, 414, 676, 679
Sau3AI GATC 6 cut(s) 118, 190, 319, 367, 373, 462
Sau96I GGNCC 1 cut(s) 89
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 1 cut(s) 255
SetI ASST 9 cut(s) 148, 154, 169, 220, 244, 477, 542, 638, 663
SfaNI GCATC 4 cut(s) 134, 206, 233, 379
SfcI CTRYAG 1 cut(s) 335
SinI GGWCC 1 cut(s) 89
Sse9I AATT 3 cut(s) 27, 348, 417
SsiI CCGC 4 cut(s) 23, 35, 85, 610
StyD4I CCNGG 1 cut(s) 253
StyI CCWWGG 2 cut(s) 540, 562
TaaI ACNGT 1 cut(s) 576
TaiI ACGT 1 cut(s) 638
TaqI TCGA 4 cut(s) 520, 579, 632, 656
TasI AATT 3 cut(s) 27, 348, 417
TfiI GAWTC 1 cut(s) 436
Tru1I TTAA 2 cut(s) 57, 351
Tru9I TTAA 2 cut(s) 57, 351
TscAI CASTG 1 cut(s) 579
TseI GCWGC 5 cut(s) 32, 143, 413, 675, 678
TspDTI ATGAA 5 cut(s) 95, 126, 144, 198, 349
TspGWI ACGGA 1 cut(s) 118
TspRI CASTG 1 cut(s) 579
Tth111I GACNNNGTC 1 cut(s) 584
VpaK11BI GGWCC 1 cut(s) 89
VspI ATTAAT 1 cut(s) 57
ZraI GACGTC 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.