Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3C

Orthogroup ID

OG0000356

Physical Location & Seq

Genomic Coordinates Reverse (-)

28729157 .. 28730505

Gene Structure 1349 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3CG285400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 747 bp

ATGGCCCATCACCACCACCCCTACGCCTGTTCTTCGATTAACAACAACCCATTTGCTGCCCAGTTTGTGCGCTCTTGCACAAATTACAGAGCCCGTGCTTCTTCGATCAATCGAGTTTTATGGACGCCTTCTGGGAGGCGTCTTATTACAGGCTCTCAAAACGGGGAGTTCACTCTCTGGAATGGTGAGTCATTCAAACATGAATTGAGGATTCAGGCTCATGATCACGCAATCAGGTCCATGGCGTGGAGTTTTGATGATGAAGATTGGATCTCTGGTGATGATGGGGGCACAATCAGGTATTGGAAGAGTAACATGATTAATGTGCTAGTCAATGAATCTGCTCACCATGAATCGGTTCGGGACCTGAGCTTCTGTCGGACTAATTTGAAGTTTTGTTCATGTTCCGATGATACTAGTGTTAAAATCTGGGATTTTGAACGGTGCCAACAGGAGAAGACATTGACCGGCCATGGTTGGAATGTGACGAGTGTCGACTGGCACCCCACAAACTCTCTAATAGCTTCGGGTGGGTGGGACAGTGTTGTCAAACTGTGGGATGCTAGAACAGGAGATAAATCTCAAATCACTCAGAATCAAAGTAATTCTGCTTTTGCTTGTCACATGAACAGTAATTTTCCTTTTCCATGTCACGGACCACCAACTATTGCTACTACTCGAGACGAAGGAACCATTGGTTCAGGAGGTGTTGGAACCATTATACCTGGTGTTGGAATTGCGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

WD40

Protein Analysis

248

Amino Acids

27.61

Weight (kDa)

6.48

Isoelectric Point (pI)

34.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_Gbeta	PF25391	23 - 198	5e-12	G protein beta WD-40 repeat protein
Beta-prop_EML_2	PF23414	25 - 110	7.5e-06	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	26 - 198	9.4e-22	CDC20/Fizzy WD40 domain
WDR55	PF24796	27 - 102	9.4e-06	WDR55
Beta-prop_IFT140_1st	PF23383	31 - 118	7.4e-10	IFT140 first beta-propeller
Beta-prop_WDR5	PF25175	32 - 119	5.1e-08	WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	32 - 195	1e-09	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	34 - 104	1.1e-07	THOC3 beta-propeller domain
Beta-prop_EIPR1	PF23609	35 - 186	2e-07	EIPR1 beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	35 - 192	1.2e-09	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2	PF23414	43 - 206	6.9e-15	Echinoderm microtubule-associated protein second beta-propeller
EIF3I	PF24805	67 - 154	2.1e-08	EIF3I
WD40_MABP1-WDR62_2nd	PF24782	77 - 190	5.6e-06	MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_1st	PF25173	80 - 210	7.2e-19	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	91 - 207	5.9e-15	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	98 - 210	3.4e-20	WDR5 beta-propeller domain
WDR55	PF24796	108 - 203	8.4e-09	WDR55
Beta-prop_TEP1_2nd	PF25047	114 - 202	6.2e-06	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	115 - 187	1.3e-09	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	116 - 205	7.8e-18	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	118 - 201	3.7e-11	WDHD1 first WD40 domain
WD40	PF00400	150 - 187	3.4e-11	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000356)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca	FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica	MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica	Prupe.2G034300_v2.0.a1
pyrus_communis	pycom02g24420 pycom07g02650
rosa_chinensis	RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata	RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora	Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii	Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa	Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha	Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana	Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	545
AccB1I	GGYRCC	2 cut(s)	444, 501
AccB7I	CCANNNNNTGG	1 cut(s)	246
AccI	GTMKAC	1 cut(s)	495
AciI	CCGC	1 cut(s)	740
AclWI	GGATC	1 cut(s)	278
AcoI	YGGCCR	1 cut(s)	469
AcyI	GRCGYC	2 cut(s)	125, 139
AfiI	CCNNNNNNNGG	4 cut(s)	246, 355, 653, 731
AgsI	TTSAA	3 cut(s)	196, 391, 440
AhlI	ACTAGT	1 cut(s)	416
AjnI	CCWGG	1 cut(s)	724
AjuI	GAANNNNNNNTTGG	2 cut(s)	678, 710
AluBI	AGCT	2 cut(s)	372, 524
AluI	AGCT	2 cut(s)	372, 524
Alw26I	GTCTC	1 cut(s)	675
AlwI	GGATC	1 cut(s)	278
Ama87I	CYCGRG	1 cut(s)	678
AoxI	GGCC	2 cut(s)	3, 469
ApeKI	GCWGC	1 cut(s)	56
AseI	ATTAAT	1 cut(s)	321
Asp700I	GAANNNNTTC	1 cut(s)	357
AspLEI	GCGC	1 cut(s)	72
AspS9I	GGNCC	4 cut(s)	4, 237, 364, 656
AsuHPI	GGTGA	3 cut(s)	197, 290, 338
AvaI	CYCGRG	1 cut(s)	678
AvaII	GGWCC	3 cut(s)	237, 364, 656
BaeGI	GKGCMC	1 cut(s)	293
BaeI	ACNNNNGTAYC	2 cut(s)	405, 438
BanI	GGYRCC	2 cut(s)	444, 501
BanII	GRGCYC	1 cut(s)	94
BbsI	GAAGAC	1 cut(s)	464
BbvI	GCAGC	1 cut(s)	43
BccI	CCATC	2 cut(s)	15, 278
BciT130I	CCWGG	1 cut(s)	726
BclI	TGATCA	1 cut(s)	223
BcoDI	GTCTC	1 cut(s)	675
BcuI	ACTAGT	1 cut(s)	416
BfaI	CTAG	3 cut(s)	329, 417, 564
BisI	GCNGC	1 cut(s)	57
BlsI	GCNGC	1 cut(s)	58
Bme1390I	CCNGG	1 cut(s)	726
Bme18I	GGWCC	3 cut(s)	237, 364, 656
BmeT110I	CYCGRG	1 cut(s)	678
BmgT120I	GGNCC	4 cut(s)	4, 237, 364, 656
BmiI	GGNNCC	5 cut(s)	365, 446, 503, 691, 715
BmrFI	CCNGG	1 cut(s)	726
BmrI	ACTGGG	1 cut(s)	55
BmsI	GCATC	1 cut(s)	550
BmuI	ACTGGG	1 cut(s)	55
BoxI	GACNNNNGTC	1 cut(s)	491
BpiI	GAAGAC	1 cut(s)	464
Bpu10I	CCTNAGC	1 cut(s)	368
BsaHI	GRCGYC	2 cut(s)	125, 139
BsaJI	CCNNGG	2 cut(s)	240, 472
Bsc4I	CCNNNNNNNGG	4 cut(s)	246, 355, 653, 731
Bse118I	RCCGGY	1 cut(s)	467
Bse1I	ACTGG	2 cut(s)	61, 503
BseBI	CCWGG	1 cut(s)	726
BseDI	CCNNGG	2 cut(s)	240, 472
BseGI	GGATG	1 cut(s)	565
BseLI	CCNNNNNNNGG	4 cut(s)	246, 355, 653, 731
BseMII	CTCAG	2 cut(s)	359, 605
BseNI	ACTGG	2 cut(s)	61, 503
BseSI	GKGCMC	1 cut(s)	293
BseXI	GCAGC	1 cut(s)	43
BshFI	GGCC	2 cut(s)	5, 471
BshNI	GGYRCC	2 cut(s)	444, 501
BsiHKCI	CYCGRG	1 cut(s)	678
BsiSI	CCGG	1 cut(s)	468
BslFI	GGGAC	2 cut(s)	377, 551
BslI	CCNNNNNNNGG	4 cut(s)	246, 355, 653, 731
BsmAI	GTCTC	1 cut(s)	675
BsmBI	CGTCTC	1 cut(s)	675
BsmFI	GGGAC	2 cut(s)	377, 551
BsnI	GGCC	2 cut(s)	5, 471
BsoBI	CYCGRG	1 cut(s)	678
Bsp1286I	GDGCHC	2 cut(s)	94, 293
Bsp143I	GATC	3 cut(s)	105, 223, 270
Bsp19I	CCATGG	2 cut(s)	240, 472
BspACI	CCGC	1 cut(s)	740
BspANI	GGCC	2 cut(s)	5, 471
BspCNI	CTCAG	2 cut(s)	360, 604
BspHI	TCATGA	1 cut(s)	220
BspLI	GGNNCC	5 cut(s)	365, 446, 503, 691, 715
BspPI	GGATC	1 cut(s)	278
BspT107I	GGYRCC	2 cut(s)	444, 501
BsrFI	RCCGGY	1 cut(s)	467
BsrI	ACTGG	2 cut(s)	61, 503
BssAI	RCCGGY	1 cut(s)	467
BssECI	CCNNGG	2 cut(s)	240, 472
BssMI	GATC	3 cut(s)	105, 223, 270
BssNI	GRCGYC	2 cut(s)	125, 139
BssT1I	CCWWGG	2 cut(s)	240, 472
Bst2UI	CCWGG	1 cut(s)	726
Bst4CI	ACNGT	4 cut(s)	444, 542, 555, 632
Bst6I	CTCTTC	1 cut(s)	302
BstACI	GRCGYC	2 cut(s)	125, 139
BstDEI	CTNAG	2 cut(s)	368, 591
BstDSI	CCRYGG	2 cut(s)	240, 472
BstF5I	GGATG	1 cut(s)	565
BstHHI	GCGC	1 cut(s)	72
BstKTI	GATC	3 cut(s)	108, 226, 273
BstMAI	GTCTC	1 cut(s)	675
BstMBI	GATC	3 cut(s)	105, 223, 270
BstNI	CCWGG	1 cut(s)	726
BstPAI	GACNNNNGTC	1 cut(s)	491
BstSCI	CCNGG	1 cut(s)	724
BstSLI	GKGCMC	1 cut(s)	293
BstV1I	GCAGC	1 cut(s)	43
BstV2I	GAAGAC	1 cut(s)	464
BstX2I	RGATCY	1 cut(s)	270
BstYI	RGATCY	1 cut(s)	270
BsuRI	GGCC	2 cut(s)	5, 471
BtgI	CCRYGG	2 cut(s)	240, 472
BtsCI	GGATG	1 cut(s)	565
BtsIMutI	CAGTG	1 cut(s)	547
CciI	TCATGA	1 cut(s)	220
CfoI	GCGC	1 cut(s)	72
Cfr10I	RCCGGY	1 cut(s)	467
Cfr13I	GGNCC	4 cut(s)	4, 237, 364, 656
CseI	GACGC	2 cut(s)	128, 133
CsiI	ACCWGGT	1 cut(s)	724
CviAII	CATG	9 cut(s)	200, 221, 241, 316, 350, 402, 473, 625, 648
CviJI	RGCY	7 cut(s)	5, 92, 153, 218, 372, 471, 524
CviKI_1	RGCY	7 cut(s)	5, 92, 153, 218, 372, 471, 524
DdeI	CTNAG	2 cut(s)	368, 591
DpnI	GATC	3 cut(s)	107, 225, 272
DpnII	GATC	3 cut(s)	105, 223, 270
DrdI	GACNNNNNNGTC	1 cut(s)	545
DseDI	GACNNNNNNGTC	1 cut(s)	545
EaeI	YGGCCR	1 cut(s)	469
Eam1104I	CTCTTC	1 cut(s)	302
EarI	CTCTTC	1 cut(s)	302
Eco130I	CCWWGG	2 cut(s)	240, 472
Eco24I	GRGCYC	1 cut(s)	94
Eco47I	GGWCC	3 cut(s)	237, 364, 656
Eco88I	CYCGRG	1 cut(s)	678
EcoO109I	RGGNCCY	1 cut(s)	364
EcoRII	CCWGG	1 cut(s)	724
EcoT14I	CCWWGG	2 cut(s)	240, 472
EcoT38I	GRGCYC	1 cut(s)	94
ErhI	CCWWGG	2 cut(s)	240, 472
Esp3I	CGTCTC	1 cut(s)	675
FaeI	CATG	9 cut(s)	203, 224, 244, 319, 353, 405, 476, 628, 651
FaqI	GGGAC	2 cut(s)	377, 551
FatI	CATG	9 cut(s)	199, 220, 240, 315, 349, 401, 472, 624, 647
FbaI	TGATCA	1 cut(s)	223
FblI	GTMKAC	1 cut(s)	495
Fnu4HI	GCNGC	1 cut(s)	57
FokI	GGATG	1 cut(s)	572
FriOI	GRGCYC	1 cut(s)	94
Fsp4HI	GCNGC	1 cut(s)	57
FspBI	CTAG	3 cut(s)	329, 417, 564
GlaI	GCGC	1 cut(s)	71
GluI	GCNGC	1 cut(s)	57
HaeIII	GGCC	2 cut(s)	5, 471
HapII	CCGG	1 cut(s)	468
HgaI	GACGC	2 cut(s)	128, 133
HhaI	GCGC	1 cut(s)	72
Hin1I	GRCGYC	2 cut(s)	125, 139
Hin1II	CATG	9 cut(s)	203, 224, 244, 319, 353, 405, 476, 628, 651
Hin6I	GCGC	1 cut(s)	70
HinP1I	GCGC	1 cut(s)	70
HincII	GTYRAC	1 cut(s)	496
HindII	GTYRAC	1 cut(s)	496
HinfI	GANTC	5 cut(s)	188, 211, 338, 353, 595
HpaII	CCGG	1 cut(s)	468
HphI	GGTGA	3 cut(s)	197, 290, 338
Hpy166II	GTNNAC	2 cut(s)	171, 496
Hpy188I	TCNGA	3 cut(s)	381, 409, 594
Hpy188III	TCNNGA	5 cut(s)	178, 221, 362, 680, 702
Hpy8I	GTNNAC	2 cut(s)	171, 496
HpyAV	CCTTC	2 cut(s)	138, 680
HpyCH4III	ACNGT	4 cut(s)	444, 542, 555, 632
HpyCH4V	TGCA	1 cut(s)	78
HpyF3I	CTNAG	2 cut(s)	368, 591
Hsp92I	GRCGYC	2 cut(s)	125, 139
Hsp92II	CATG	9 cut(s)	203, 224, 244, 319, 353, 405, 476, 628, 651
HspAI	GCGC	1 cut(s)	70
Ksp22I	TGATCA	1 cut(s)	223
Kzo9I	GATC	3 cut(s)	105, 223, 270
Lsp1109I	GCAGC	1 cut(s)	43
LweI	GCATC	1 cut(s)	550
MabI	ACCWGGT	1 cut(s)	724
MaeI	CTAG	3 cut(s)	329, 417, 564
MaeIII	GTNAC	4 cut(s)	311, 484, 620, 650
MalI	GATC	3 cut(s)	107, 225, 272
MboI	GATC	3 cut(s)	105, 223, 270
MboII	GAAGA	5 cut(s)	24, 93, 275, 319, 469
MflI	RGATCY	1 cut(s)	270
MhlI	GDGCHC	2 cut(s)	94, 293
MluCI	AATT	6 cut(s)	82, 203, 385, 604, 634, 735
MlyI	GAGTC	1 cut(s)	197
MmeI	TCCRAC	4 cut(s)	359, 458, 691, 712
MnlI	CCTC	3 cut(s)	129, 201, 698
MroXI	GAANNNNTTC	1 cut(s)	357
MseI	TTAA	4 cut(s)	39, 321, 423, 745
MspI	CCGG	1 cut(s)	468
MspR9I	CCNGG	1 cut(s)	726
MvaI	CCWGG	1 cut(s)	726
NcoI	CCATGG	2 cut(s)	240, 472
NdeII	GATC	3 cut(s)	105, 223, 270
NlaIII	CATG	9 cut(s)	203, 224, 244, 319, 353, 405, 476, 628, 651
NlaIV	GGNNCC	5 cut(s)	365, 446, 503, 691, 715
NmuCI	GTSAC	3 cut(s)	484, 620, 650
PaeR7I	CTCGAG	1 cut(s)	678
PagI	TCATGA	1 cut(s)	220
PdmI	GAANNNNTTC	1 cut(s)	357
PfeI	GAWTC	4 cut(s)	211, 338, 353, 595
PflMI	CCANNNNNTGG	1 cut(s)	246
PkrI	GCNGC	1 cut(s)	58
PleI	GAGTC	1 cut(s)	196
PpsI	GAGTC	1 cut(s)	196
PpuMI	RGGWCCY	1 cut(s)	364
PshAI	GACNNNNGTC	1 cut(s)	491
PshBI	ATTAAT	1 cut(s)	321
Psp5II	RGGWCCY	1 cut(s)	364
Psp6I	CCWGG	1 cut(s)	724
PspGI	CCWGG	1 cut(s)	724
PspN4I	GGNNCC	5 cut(s)	365, 446, 503, 691, 715
PspPI	GGNCC	4 cut(s)	4, 237, 364, 656
PspPPI	RGGWCCY	1 cut(s)	364
PsuI	RGATCY	1 cut(s)	270
SalI	GTCGAC	1 cut(s)	494
SaqAI	TTAA	4 cut(s)	39, 321, 423, 745
SatI	GCNGC	1 cut(s)	57
Sau3AI	GATC	3 cut(s)	105, 223, 270
Sau96I	GGNCC	4 cut(s)	4, 237, 364, 656
SchI	GAGTC	1 cut(s)	197
ScrFI	CCNGG	1 cut(s)	726
SduI	GDGCHC	2 cut(s)	94, 293
SetI	ASST	7 cut(s)	239, 302, 369, 374, 526, 709, 727
SexAI	ACCWGGT	1 cut(s)	724
SfaNI	GCATC	1 cut(s)	550
Sfr274I	CTCGAG	1 cut(s)	678
SinI	GGWCC	3 cut(s)	237, 364, 656
SlaI	CTCGAG	1 cut(s)	678
SmlI	CTYRAG	1 cut(s)	678
SmoI	CTYRAG	1 cut(s)	678
SpeI	ACTAGT	1 cut(s)	416
Sse9I	AATT	6 cut(s)	82, 203, 385, 604, 634, 735
SsiI	CCGC	1 cut(s)	740
SspMI	CTAG	3 cut(s)	329, 417, 564
StyD4I	CCNGG	1 cut(s)	724
StyI	CCWWGG	2 cut(s)	240, 472
TaaI	ACNGT	4 cut(s)	444, 542, 555, 632
TaqI	TCGA	5 cut(s)	35, 104, 112, 495, 679
TasI	AATT	6 cut(s)	82, 203, 385, 604, 634, 735
TfiI	GAWTC	4 cut(s)	211, 338, 353, 595
Tru1I	TTAA	4 cut(s)	39, 321, 423, 745
Tru9I	TTAA	4 cut(s)	39, 321, 423, 745
TscAI	CASTG	1 cut(s)	547
TseFI	GTSAC	3 cut(s)	484, 620, 650
TseI	GCWGC	1 cut(s)	56
Tsp45I	GTSAC	3 cut(s)	484, 620, 650
TspDTI	ATGAA	6 cut(s)	216, 276, 351, 366, 390, 641
TspGWI	ACGGA	1 cut(s)	669
TspRI	CASTG	1 cut(s)	547
Van91I	CCANNNNNTGG	1 cut(s)	246
VpaK11BI	GGWCC	3 cut(s)	237, 364, 656
VspI	ATTAAT	1 cut(s)	321
XhoI	CTCGAG	1 cut(s)	678
XmiI	GTMKAC	1 cut(s)	495
XmnI	GAANNNNTTC	1 cut(s)	357
XspI	CTAG	3 cut(s)	329, 417, 564

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.