Rh5AG083200

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000356
Physical Location & Seq
Forward (+)
7158319 .. 7159212
894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG083200.1

Sequence Viewer

Length: 894 bp
ATGGAGTGGAGTTATGATGGTGAGAATTGGATTTCTGGTGATGATGGGGGATCAATAAAGTATTGGACGAGTAACATGAACAATGTGCTAGTCAATGAATCTGCTCACCGAGAATCGGTTCGGGACTTGAGCTTTTGTAGGACTAATTTGAAGTTCTGTTCATGTTCGGATGATACTTGTGTGAAAATCTGGGATTATGAAAGGTGCCAAGAAGAGTACAGATTGACTGGCCATGGTTGGAATGTGAAGAGTGTTGACTGGCACCCTACAAAGTCCTTACTAGCTTCAGGTGGGAAAGACAGTGTTGTCAAACTGTGGGATGCTAGGACAGGGAGAGAGCTTTGTTCATTTCATGATCACAAAAATTGGGTGCAATCTGTTAAGTGGAGCCGAAATGGTAACTGGCTGCTAACTGCTTCCAAGGATCAAGTCATTAAGCTTTACGACATGAGGGCTATGAAGGAACTTGAATCATTCCACGGGCATCGGAATGAAGTGACTGCTATAGCTTGGCATCCTTTTCATGAAGAATATTTTGTCAGTGGGAGTAGTGATGGATCCATTTTCCATTGGCTTGTTGGGCATGAAACTCCCCAGGTTGAAGTTCCTAATGCACACAGTAACAATCACAATAACAGTGTGTGGGATCTCCAATGGCATCCTATTGGTCATATGCTTTGCAGTGGTAGCAATGATCGCACAACAAAGTTTTGGTGCAGAAATAGGCCAGGAGATAAATGTAACATCAGTCAGAATCAAAGTATTGGTGATCGAAGTTCTGCTTTTGCTGGTCACATGACTGGTAATTTTCCATTTCCATTACATGAAGGACAACCAAAAATTGCTACTCGAAACCAAGGAACCATTATTCCAGGTGTTGGATTTGCAATTTAA

Protein Analysis

297

Amino Acids

33.96

Weight (kDa)

6.48

Isoelectric Point (pI)

25.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 1 - 124 2.2e-21 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 1 - 107 5.2e-11 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 3 - 143 1.9e-26 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 3 - 133 4.4e-23 WDR5 beta-propeller domain
WD40_Prp19 PF24814 4 - 159 1e-25 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 6 - 116 8.7e-15 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 10 - 170 1.8e-16 TEP-1 second beta-propeller
WDR55 PF24796 20 - 191 2.4e-18 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 28 - 191 4.2e-18 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 34 - 115 3e-09 WDHD1 first WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 35 - 104 5e-06 CAF1B/HIR1 beta-propeller domain
WD40_Gbeta PF25391 41 - 169 3.6e-17 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 48 - 192 3.9e-22 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 52 - 139 3.1e-09 WDR90/POC16, second beta-propeller
Beta-prop_SCAP PF24017 53 - 149 5.6e-06 SCAP Beta-propeller
Beta-prop_WDR3_1st PF25173 58 - 191 7.8e-30 WDR3 first beta-propeller domain
Beta-prop_IFT140_1st PF23383 60 - 146 2.6e-07 IFT140 first beta-propeller
WD40 PF00400 72 - 107 4.9e-09 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 74 - 194 4.2e-13 WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 75 - 156 1.2e-11 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 76 - 237 1.8e-14 EIF3I
Beta-prop_EML PF23409 76 - 181 1.1e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin PF25460 81 - 190 1.4e-07 Aladin seven-bladed propeller
WD40_MABP1-WDR62_2nd PF24782 82 - 238 4.9e-10 MABP1/WDR62 second WD40 domain
Beta-prop_WDR19_1st PF23389 85 - 235 8.4e-08 WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 98 - 239 1.7e-10 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 103 - 238 6.6e-22 WDR3 first beta-propeller domain
Beta-prop_EIPR1 PF23609 109 - 238 7.5e-08 EIPR1 beta-propeller
WD40 PF00400 112 - 149 2.9e-08 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 112 - 242 4e-22 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 118 - 240 5e-20 WDR5 beta-propeller domain
Beta-prop_DCAF4 PF23761 120 - 180 5.7e-06 DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_THOC3 PF25174 120 - 238 8.8e-22 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 124 - 242 2.5e-13 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 136 - 231 3.4e-07 WDR90/POC16, second beta-propeller
WD40 PF00400 154 - 191 3.9e-06 WD domain, G-beta repeat
WD40_Prp19 PF24814 155 - 238 3.8e-10 Prp19 WD40 domain
WD40 PF00400 209 - 238 1e-05 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica Prupe.2G034300_v2.0.a1
pyrus_communis pycom02g24420 pycom07g02650
rosa_chinensis RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 305
AccB1I GGYRCC 2 cut(s) 204, 261
AccB7I CCANNNNNTGG 1 cut(s) 878
AclWI GGATC 5 cut(s) 58, 432, 552, 565, 654
AcoI YGGCCR 1 cut(s) 229
AcuI CTGAAG 1 cut(s) 270
AfaI GTAC 1 cut(s) 218
AfiI CCNNNNNNNGG 2 cut(s) 115, 878
AgsI TTSAA 3 cut(s) 151, 470, 602
AjnI CCWGG 3 cut(s) 594, 727, 871
AloI GAACNNNNNNTCC 2 cut(s) 853, 885
AluBI AGCT 5 cut(s) 132, 284, 340, 439, 509
AluI AGCT 5 cut(s) 132, 284, 340, 439, 509
AlwI GGATC 5 cut(s) 58, 432, 552, 565, 654
AoxI GGCC 2 cut(s) 229, 725
ApeKI GCWGC 1 cut(s) 406
Asp700I GAANNNNTTC 1 cut(s) 117
AsuHPI GGTGA 4 cut(s) 32, 50, 98, 779
BaeI ACNNNNGTAYC 2 cut(s) 165, 198
BalI TGGCCA 1 cut(s) 231
BamHI GGATCC 1 cut(s) 557
BanI GGYRCC 2 cut(s) 204, 261
BbvI GCAGC 1 cut(s) 393
BccI CCATC 3 cut(s) 11, 38, 548
BciT130I CCWGG 3 cut(s) 596, 729, 873
BclI TGATCA 1 cut(s) 355
BfaI CTAG 3 cut(s) 89, 281, 324
BfmI CTRYAG 1 cut(s) 504
BisI GCNGC 1 cut(s) 407
BlsI GCNGC 1 cut(s) 408
Bme1390I CCNGG 3 cut(s) 596, 729, 873
BmiI GGNNCC 5 cut(s) 206, 263, 389, 559, 862
BmrFI CCNGG 3 cut(s) 596, 729, 873
BmsI GCATC 4 cut(s) 310, 493, 523, 667
BpuEI CTTGAG 1 cut(s) 148
BsaJI CCNNGG 5 cut(s) 232, 420, 478, 594, 856
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 878
Bse1I ACTGG 4 cut(s) 232, 263, 407, 805
Bse3DI GCAATG 1 cut(s) 697
BseBI CCWGG 3 cut(s) 596, 729, 873
BseDI CCNNGG 5 cut(s) 232, 420, 478, 594, 856
BseGI GGATG 4 cut(s) 175, 325, 514, 658
BseLI CCNNNNNNNGG 2 cut(s) 115, 878
BseMI GCAATG 1 cut(s) 697
BseNI ACTGG 4 cut(s) 232, 263, 407, 805
BseXI GCAGC 1 cut(s) 393
BsgI GTGCAG 1 cut(s) 736
BshFI GGCC 2 cut(s) 231, 727
BshNI GGYRCC 2 cut(s) 204, 261
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 2 cut(s) 115, 878
BsmFI GGGAC 1 cut(s) 137
BsnI GGCC 2 cut(s) 231, 727
Bsp143I GATC 7 cut(s) 50, 355, 424, 557, 646, 694, 769
Bsp19I CCATGG 1 cut(s) 232
BspANI GGCC 2 cut(s) 231, 727
BspHI TCATGA 2 cut(s) 352, 523
BspLI GGNNCC 5 cut(s) 206, 263, 389, 559, 862
BspPI GGATC 5 cut(s) 58, 432, 552, 565, 654
BspT107I GGYRCC 2 cut(s) 204, 261
BsrDI GCAATG 1 cut(s) 697
BsrI ACTGG 4 cut(s) 232, 263, 407, 805
BssECI CCNNGG 5 cut(s) 232, 420, 478, 594, 856
BssMI GATC 7 cut(s) 50, 355, 424, 557, 646, 694, 769
BssT1I CCWWGG 3 cut(s) 232, 420, 856
Bst2UI CCWGG 3 cut(s) 596, 729, 873
Bst4CI ACNGT 4 cut(s) 302, 315, 620, 638
Bst6I CTCTTC 2 cut(s) 207, 242
BstDSI CCRYGG 2 cut(s) 232, 478
BstF5I GGATG 4 cut(s) 175, 325, 514, 658
BstKTI GATC 7 cut(s) 53, 358, 427, 560, 649, 697, 772
BstMBI GATC 7 cut(s) 50, 355, 424, 557, 646, 694, 769
BstMWI GCNNNNNNNGC 3 cut(s) 580, 687, 696
BstNI CCWGG 3 cut(s) 596, 729, 873
BstSCI CCNGG 3 cut(s) 594, 727, 871
BstSFI CTRYAG 1 cut(s) 504
BstV1I GCAGC 1 cut(s) 393
BstX2I RGATCY 2 cut(s) 557, 646
BstYI RGATCY 2 cut(s) 557, 646
BsuRI GGCC 2 cut(s) 231, 727
BtgI CCRYGG 2 cut(s) 232, 478
BtsCI GGATG 4 cut(s) 175, 325, 514, 658
BtsI GCAGTG 1 cut(s) 688
BtsIMutI CAGTG 4 cut(s) 307, 547, 643, 688
CciI TCATGA 2 cut(s) 352, 523
Csp6I GTAC 1 cut(s) 217
CviAII CATG 9 cut(s) 76, 162, 233, 353, 448, 524, 584, 796, 824
CviQI GTAC 1 cut(s) 217
DpnI GATC 7 cut(s) 52, 357, 426, 559, 648, 696, 771
DpnII GATC 7 cut(s) 50, 355, 424, 557, 646, 694, 769
DrdI GACNNNNNNGTC 1 cut(s) 305
DseDI GACNNNNNNGTC 1 cut(s) 305
EaeI YGGCCR 1 cut(s) 229
Eam1104I CTCTTC 2 cut(s) 207, 242
EarI CTCTTC 2 cut(s) 207, 242
Eco130I CCWWGG 3 cut(s) 232, 420, 856
Eco57I CTGAAG 1 cut(s) 270
EcoRII CCWGG 3 cut(s) 594, 727, 871
EcoT14I CCWWGG 3 cut(s) 232, 420, 856
ErhI CCWWGG 3 cut(s) 232, 420, 856
FaeI CATG 9 cut(s) 79, 165, 236, 356, 451, 527, 587, 799, 827
FalI AAGNNNNNCTT 2 cut(s) 766, 798
FaqI GGGAC 1 cut(s) 137
FatI CATG 9 cut(s) 75, 161, 232, 352, 447, 523, 583, 795, 823
FauNDI CATATG 1 cut(s) 672
FbaI TGATCA 1 cut(s) 355
Fnu4HI GCNGC 1 cut(s) 407
FokI GGATG 4 cut(s) 182, 332, 501, 645
Fsp4HI GCNGC 1 cut(s) 407
FspBI CTAG 3 cut(s) 89, 281, 324
GluI GCNGC 1 cut(s) 407
HaeIII GGCC 2 cut(s) 231, 727
Hin1II CATG 9 cut(s) 79, 165, 236, 356, 451, 527, 587, 799, 827
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 4 cut(s) 98, 113, 470, 754
HphI GGTGA 4 cut(s) 32, 50, 98, 779
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 3 cut(s) 169, 489, 753
Hpy188III TCNNGA 3 cut(s) 122, 353, 524
Hpy8I GTNNAC 1 cut(s) 256
HpyAV CCTTC 2 cut(s) 454, 821
HpyCH4III ACNGT 4 cut(s) 302, 315, 620, 638
HpyCH4V TGCA 5 cut(s) 373, 614, 681, 717, 887
HpyF10VI GCNNNNNNNGC 3 cut(s) 580, 687, 696
Hsp92II CATG 9 cut(s) 79, 165, 236, 356, 451, 527, 587, 799, 827
Ksp22I TGATCA 1 cut(s) 355
Kzo9I GATC 7 cut(s) 50, 355, 424, 557, 646, 694, 769
LmnI GCTCC 1 cut(s) 387
Lsp1109I GCAGC 1 cut(s) 393
LweI GCATC 4 cut(s) 310, 493, 523, 667
MaeI CTAG 3 cut(s) 89, 281, 324
MaeIII GTNAC 6 cut(s) 71, 398, 496, 620, 740, 791
MalI GATC 7 cut(s) 52, 357, 426, 559, 648, 696, 771
MboI GATC 7 cut(s) 50, 355, 424, 557, 646, 694, 769
MboII GAAGA 3 cut(s) 224, 259, 539
MflI RGATCY 2 cut(s) 557, 646
MlsI TGGCCA 1 cut(s) 231
MluCI AATT 6 cut(s) 25, 145, 364, 805, 840, 888
MluNI TGGCCA 1 cut(s) 231
MmeI TCCRAC 2 cut(s) 218, 859
MnlI CCTC 1 cut(s) 444
Mox20I TGGCCA 1 cut(s) 231
MroXI GAANNNNTTC 1 cut(s) 117
MscI TGGCCA 1 cut(s) 231
MseI TTAA 3 cut(s) 381, 435, 892
MslI CAYNNNNRTG 1 cut(s) 489
Msp20I TGGCCA 1 cut(s) 231
MspR9I CCNGG 3 cut(s) 596, 729, 873
MvaI CCWGG 3 cut(s) 596, 729, 873
MwoI GCNNNNNNNGC 3 cut(s) 580, 687, 696
NcoI CCATGG 1 cut(s) 232
NdeI CATATG 1 cut(s) 672
NdeII GATC 7 cut(s) 50, 355, 424, 557, 646, 694, 769
NlaIII CATG 9 cut(s) 79, 165, 236, 356, 451, 527, 587, 799, 827
NlaIV GGNNCC 5 cut(s) 206, 263, 389, 559, 862
NmuCI GTSAC 2 cut(s) 496, 791
PagI TCATGA 2 cut(s) 352, 523
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 4 cut(s) 98, 113, 470, 754
PflMI CCANNNNNTGG 1 cut(s) 878
PkrI GCNGC 1 cut(s) 408
Psp6I CCWGG 3 cut(s) 594, 727, 871
PspGI CCWGG 3 cut(s) 594, 727, 871
PspN4I GGNNCC 5 cut(s) 206, 263, 389, 559, 862
PsuI RGATCY 2 cut(s) 557, 646
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
RseI CAYNNNNRTG 1 cut(s) 489
SaqAI TTAA 3 cut(s) 381, 435, 892
SatI GCNGC 1 cut(s) 407
Sau3AI GATC 7 cut(s) 50, 355, 424, 557, 646, 694, 769
ScrFI CCNGG 3 cut(s) 596, 729, 873
SetI ASST 9 cut(s) 134, 206, 286, 292, 342, 441, 511, 600, 877
SfaNI GCATC 4 cut(s) 310, 493, 523, 667
SfcI CTRYAG 1 cut(s) 504
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
Sse9I AATT 6 cut(s) 25, 145, 364, 805, 840, 888
SspI AATATT 1 cut(s) 533
SspMI CTAG 3 cut(s) 89, 281, 324
StyD4I CCNGG 3 cut(s) 594, 727, 871
StyI CCWWGG 3 cut(s) 232, 420, 856
TaaI ACNGT 4 cut(s) 302, 315, 620, 638
TaqI TCGA 2 cut(s) 772, 850
TasI AATT 6 cut(s) 25, 145, 364, 805, 840, 888
TatI WGTACW 1 cut(s) 216
TfiI GAWTC 4 cut(s) 98, 113, 470, 754
Tru1I TTAA 3 cut(s) 381, 435, 892
Tru9I TTAA 3 cut(s) 381, 435, 892
TscAI CASTG 4 cut(s) 307, 547, 643, 688
TseFI GTSAC 2 cut(s) 496, 791
TseI GCWGC 1 cut(s) 406
Tsp45I GTSAC 2 cut(s) 496, 791
TspRI CASTG 4 cut(s) 307, 547, 643, 688
Van91I CCANNNNNTGG 1 cut(s) 878
XcmI CCANNNNNNNNNTGG 1 cut(s) 575
XmnI GAANNNNTTC 1 cut(s) 117
XspI CTAG 3 cut(s) 89, 281, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.