FvH4_6g13322

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000451
Physical Location & Seq
Forward (+)
8107230 .. 8109767
2538 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g13322.t1

Sequence Viewer

Length: 1068 bp
ATGGAATCAAAGTCAGCCCTTTATGCCCCATCAGCGCCACTCCCAAATGTTCAAGAAATGGTGAAGATCGACCCTTCTCAGGTACCGGCAAATTACATCAGGAATGAAACAGACATTGCAAATGATGCAGCCTTCTGTCATCATCTTTCTTCTGAGGTTCCCATCATTGATCTTTATCTGCTCTCAAAAGGGCACCAAGAGGAGCTTACCAAGCTGGACCAGGCTTGCAAGGAATGGGGTTTCTTTCTGGTGGTGAATCATGGAGTGGCAACAGAAGTGACCAACGGCTTGAAGGAAGCGGCGGAAAAGTTCTTTGAACTTCCATTAGAAGAGAAGAACAAAATTGCTATCCCACCGAATGACATACAAGGCTATGGACATACCCAGGTGGATCGCAAGGTGCTGGACTGGTCTGACAAGCTTGTTCTTCTCGTTTACCCACATCAGTACAGAAAGCTCGATCTTTGGCCACCAACTCCATTCAAGGATGCTATTGAGAAATACTCTGGTGATGTTAGGAGAGTTGCAGAGGAGCTTATCAGGTCTCTATCTGTAATCATGGGGATGGAGAAGGATGCTCTCCTCAGACTACACCAAGAGTTGGTCCAACCTCTGTCTGTGGCTTACTATCCTCAGTGCTCAATGCCTGACAAAGTGCTTGGTCTAAGTGCGCACTCCGACAAAGGGACCTTGGCCATAGTTATGCAGGAAGATGATGTGACGGGACTACAGATCAAACACAATGGAAAATGGGTACCGATCAAGCCAATTCATGATGCCTTTGTTGTGAATGTTGGAGATATTATTGAGATGTGGAGTAATGGGAAGTATAAGAGCATTGAACACAGAGTTGTGACAAACGAAAGCAAGGCAAGGATCTCTTATTCTACTTTCTTTTTACCTCATCAAAATGCTGAAATTGAACCTCTTGACCAAATGGTGCTCGAGTCGCCCGGTTCCCTTCCATTGTACAAGAAAGTCACCTATGGGGACTATCTAAGGAAAACCAAGGATATGAAATCTGAAGGGAAGACGCATGTCAGTGAAGTTGCCAAGGTCGAAAGTTAG

Protein Analysis

356

Amino Acids

40.12

Weight (kDa)

5.74

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 53 - 158 9.2e-27 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 205 - 300 9.4e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000451)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13280 FvH4_6g13281 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13300 FvH4_6g13322
malus_domestica MD04G1132000.v1.1 MD04G1132100.v1.1 MD04G1132400.v1.1 MD12G1145100.v1.1 MD12G1145200.v1.1
prunus_persica Prupe.6G258600_v2.0.a1 Prupe.6G258700_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258900_v2.0.a1
pyrus_communis pycom04g12010 pycom04g12020
rosa_chinensis RchiOBHm_Chr3g0466131 RchiOBHm_Chr3g0466151 RchiOBHm_Chr3g0466161 RchiOBHm_Chr3g0466171 RchiOBHm_Chr3g0466181 RchiOBHm_Chr3g0466201 RchiOBHm_Chr3g0466211 RchiOBHm_Chr6g0251841
rosa_laevigata RLG00000015425 RLG00000024596 RLG00000024599 RLG00000024600 RLG00000024601 RLG00000024602
rosa_multiflora Rmu_co8112718.1_g000001 Rmu_co8178858.1_g000001 Rmu_co8388385.1_g000001 Rmu_sc0000350.1_g000002 Rmu_sc0000350.1_g000003 Rmu_sc0000350.1_g000004 Rmu_sc0000350.1_g000006 Rmu_sc0000350.1_g000011 Rmu_sc0002249.1_g000004 Rmu_sc0007597.1_g000012 Rmu_sc0012177.1_g000002 Rmu_sc0012177.1_g000006 Rmu_sc0012177.1_g000007 Rmu_sc0022084.1_g000003 Rmu_ssc0000303.1_g000004
rosa_roxburghii Rroxscaffold_6G00414720 Rroxscaffold_6G00414730 Rroxscaffold_6G00414740 Rroxscaffold_6G00414750 Rroxscaffold_6G00414760 Rroxscaffold_6G00414770 Rroxscaffold_6G00414780 Rroxscaffold_6G00414790
rosa_rugosa Rorug03G0079600 Rorug03G0079600 Rorug03G0079800 Rorug03G0080100 Rorug03G0080200 Rorug03G0080300 Rorug03G0080400 Rorug05G0504900
rosa_samantha Rh3BG147200 Rh3BG147300 Rh3BG147400 Rh3BG147500 Rh3BG147600 Rh3CG147900 Rh3CG148000 Rh3CG148100 Rh3CG148200 Rh3CG148300 Rh3CG148500 Rh3CG148600 Rh3DG147800 Rh3DG147900 Rh3DG148100 Rh3DG148300 Rh4BG237100 Rh6AG015600 Rh6AG015700 Rh6BG013700 Rh6CG069500
rosa_wichuraiana Rw3G011870 Rw3G011880 Rw3G011890 Rw3G011900 Rw3G011910 Rw3G011930 Rw6G001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 672
Acc65I GGTACC 2 cut(s) 82, 754
AccB1I GGYRCC 3 cut(s) 82, 192, 754
AccB7I CCANNNNNTGG 1 cut(s) 601
AciI CCGC 2 cut(s) 299, 302
AclWI GGATC 2 cut(s) 399, 884
AcoI YGGCCR 2 cut(s) 467, 693
AcuI CTGAAG 1 cut(s) 1044
AfaI GTAC 4 cut(s) 84, 449, 756, 971
AfiI CCNNNNNNNGG 3 cut(s) 79, 601, 684
AgsI TTSAA 6 cut(s) 53, 292, 317, 484, 842, 923
AjnI CCWGG 2 cut(s) 219, 384
AluBI AGCT 5 cut(s) 205, 214, 421, 457, 535
AluI AGCT 5 cut(s) 205, 214, 421, 457, 535
Alw21I GWGCWC 2 cut(s) 641, 945
Alw26I GTCTC 1 cut(s) 549
AlwI GGATC 2 cut(s) 399, 884
Ama87I CYCGRG 1 cut(s) 944
AoxI GGCC 2 cut(s) 467, 693
ApeKI GCWGC 1 cut(s) 128
Asp718I GGTACC 2 cut(s) 82, 754
AspLEI GCGC 2 cut(s) 37, 673
AspS9I GGNCC 3 cut(s) 217, 604, 687
AsuC2I CCSGG 1 cut(s) 954
AsuHPI GGTGA 4 cut(s) 73, 265, 521, 973
AvaI CYCGRG 1 cut(s) 944
AvaII GGWCC 3 cut(s) 217, 604, 687
BaeGI GKGCMC 1 cut(s) 195
BalI TGGCCA 2 cut(s) 469, 695
BanI GGYRCC 3 cut(s) 82, 192, 754
BbsI GAAGAC 1 cut(s) 1037
Bbv12I GWGCWC 2 cut(s) 641, 945
BbvI GCAGC 1 cut(s) 140
BccI CCATC 3 cut(s) 37, 170, 559
BceAI ACGGC 1 cut(s) 301
BciT130I CCWGG 2 cut(s) 221, 386
BcnI CCSGG 1 cut(s) 954
BcoDI GTCTC 1 cut(s) 549
BfmI CTRYAG 1 cut(s) 728
BfoI RGCGCY 1 cut(s) 38
BisI GCNGC 2 cut(s) 129, 300
BlsI GCNGC 2 cut(s) 130, 301
Bme1390I CCNGG 3 cut(s) 221, 386, 954
Bme18I GGWCC 3 cut(s) 217, 604, 687
BmeT110I CYCGRG 1 cut(s) 944
BmgT120I GGNCC 3 cut(s) 217, 604, 687
BmiI GGNNCC 6 cut(s) 84, 159, 194, 688, 756, 958
BmrFI CCNGG 3 cut(s) 221, 386, 954
BmsI GCATC 4 cut(s) 115, 478, 565, 766
BoxI GACNNNNGTC 1 cut(s) 1037
BpiI GAAGAC 1 cut(s) 1037
BplI GAGNNNNNCTC 2 cut(s) 488, 520
BpuMI CCSGG 1 cut(s) 954
BsaBI GATNNNNATC 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 549
BsaJI CCNNGG 4 cut(s) 384, 690, 1008, 1053
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 601, 684
Bse118I RCCGGY 1 cut(s) 85
Bse1I ACTGG 1 cut(s) 413
Bse3DI GCAATG 1 cut(s) 114
Bse8I GATNNNNATC 1 cut(s) 174
BseBI CCWGG 2 cut(s) 221, 386
BseDI CCNNGG 4 cut(s) 384, 690, 1008, 1053
BseGI GGATG 3 cut(s) 493, 570, 580
BseJI GATNNNNATC 1 cut(s) 174
BseLI CCNNNNNNNGG 3 cut(s) 79, 601, 684
BseMI GCAATG 1 cut(s) 114
BseMII CTCAG 4 cut(s) 92, 144, 598, 647
BseNI ACTGG 1 cut(s) 413
BseRI GAGGAG 3 cut(s) 215, 545, 572
BseSI GKGCMC 1 cut(s) 195
BseXI GCAGC 1 cut(s) 140
BshFI GGCC 2 cut(s) 469, 695
BshNI GGYRCC 3 cut(s) 82, 192, 754
BsiHKAI GWGCWC 2 cut(s) 641, 945
BsiHKCI CYCGRG 1 cut(s) 944
BsiSI CCGG 2 cut(s) 86, 954
BslFI GGGAC 3 cut(s) 700, 738, 1004
BslI CCNNNNNNNGG 3 cut(s) 79, 601, 684
BsmAI GTCTC 1 cut(s) 549
BsmFI GGGAC 3 cut(s) 700, 738, 1004
BsnI GGCC 2 cut(s) 469, 695
Bso31I GGTCTC 1 cut(s) 549
BsoBI CYCGRG 1 cut(s) 944
Bsp1286I GDGCHC 3 cut(s) 195, 641, 945
Bsp1407I TGTACA 1 cut(s) 969
Bsp143I GATC 7 cut(s) 66, 169, 391, 460, 732, 759, 876
BspACI CCGC 2 cut(s) 299, 302
BspANI GGCC 2 cut(s) 469, 695
BspCNI CTCAG 4 cut(s) 91, 145, 597, 646
BspHI TCATGA 1 cut(s) 772
BspLI GGNNCC 6 cut(s) 84, 159, 194, 688, 756, 958
BspPI GGATC 2 cut(s) 399, 884
BspT107I GGYRCC 3 cut(s) 82, 192, 754
BspTNI GGTCTC 1 cut(s) 549
BsrDI GCAATG 1 cut(s) 114
BsrFI RCCGGY 1 cut(s) 85
BsrGI TGTACA 1 cut(s) 969
BsrI ACTGG 1 cut(s) 413
BssAI RCCGGY 1 cut(s) 85
BssECI CCNNGG 4 cut(s) 384, 690, 1008, 1053
BssMI GATC 7 cut(s) 66, 169, 391, 460, 732, 759, 876
BssT1I CCWWGG 3 cut(s) 690, 1008, 1053
Bst2UI CCWGG 2 cut(s) 221, 386
Bst6I CTCTTC 1 cut(s) 324
BstAPI GCANNNNNTGC 1 cut(s) 125
BstAUI TGTACA 1 cut(s) 969
BstC8I GCNNGC 1 cut(s) 226
BstDEI CTNAG 6 cut(s) 78, 153, 584, 633, 665, 998
BstF5I GGATG 3 cut(s) 493, 570, 580
BstH2I RGCGCY 1 cut(s) 38
BstHHI GCGC 2 cut(s) 37, 673
BstKTI GATC 7 cut(s) 69, 172, 394, 463, 735, 762, 879
BstMAI GTCTC 1 cut(s) 549
BstMBI GATC 7 cut(s) 66, 169, 391, 460, 732, 759, 876
BstMWI GCNNNNNNNGC 5 cut(s) 23, 32, 125, 211, 949
BstNI CCWGG 2 cut(s) 221, 386
BstNSI RCATGY 1 cut(s) 1040
BstPAI GACNNNNGTC 1 cut(s) 1037
BstSCI CCNGG 3 cut(s) 219, 384, 952
BstSFI CTRYAG 1 cut(s) 728
BstSLI GKGCMC 1 cut(s) 195
BstV1I GCAGC 1 cut(s) 140
BstV2I GAAGAC 1 cut(s) 1037
BstX2I RGATCY 1 cut(s) 876
BstYI RGATCY 1 cut(s) 876
BsuRI GGCC 2 cut(s) 469, 695
BtsCI GGATG 3 cut(s) 493, 570, 580
BtsIMutI CAGTG 2 cut(s) 641, 1048
Cac8I GCNNGC 1 cut(s) 226
CciI TCATGA 1 cut(s) 772
CfoI GCGC 2 cut(s) 37, 673
Cfr10I RCCGGY 1 cut(s) 85
Cfr13I GGNCC 3 cut(s) 217, 604, 687
CseI GACGC 1 cut(s) 1042
Csp6I GTAC 4 cut(s) 83, 448, 755, 970
CviAII CATG 4 cut(s) 260, 559, 773, 1037
CviQI GTAC 4 cut(s) 83, 448, 755, 970
DdeI CTNAG 6 cut(s) 78, 153, 584, 633, 665, 998
DpnI GATC 7 cut(s) 68, 171, 393, 462, 734, 761, 878
DpnII GATC 7 cut(s) 66, 169, 391, 460, 732, 759, 876
EaeI YGGCCR 2 cut(s) 467, 693
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
EciI GGCGGA 1 cut(s) 317
Eco130I CCWWGG 3 cut(s) 690, 1008, 1053
Eco31I GGTCTC 1 cut(s) 549
Eco47I GGWCC 3 cut(s) 217, 604, 687
Eco57I CTGAAG 1 cut(s) 1044
Eco88I CYCGRG 1 cut(s) 944
EcoO109I RGGNCCY 1 cut(s) 687
EcoRII CCWGG 2 cut(s) 219, 384
EcoT14I CCWWGG 3 cut(s) 690, 1008, 1053
ErhI CCWWGG 3 cut(s) 690, 1008, 1053
FaeI CATG 4 cut(s) 263, 562, 776, 1040
FalI AAGNNNNNCTT 4 cut(s) 189, 221, 865, 897
FaqI GGGAC 3 cut(s) 700, 738, 1004
FatI CATG 4 cut(s) 259, 558, 772, 1036
Fnu4HI GCNGC 2 cut(s) 129, 300
FokI GGATG 3 cut(s) 500, 577, 587
Fsp4HI GCNGC 2 cut(s) 129, 300
FspAI RTGCGCAY 1 cut(s) 672
FspI TGCGCA 1 cut(s) 672
GlaI GCGC 2 cut(s) 36, 672
GluI GCNGC 2 cut(s) 129, 300
HaeII RGCGCY 1 cut(s) 38
HaeIII GGCC 2 cut(s) 469, 695
HapII CCGG 2 cut(s) 86, 954
HgaI GACGC 1 cut(s) 1042
HhaI GCGC 2 cut(s) 37, 673
Hin1II CATG 4 cut(s) 263, 562, 776, 1040
Hin6I GCGC 2 cut(s) 35, 671
HinP1I GCGC 2 cut(s) 35, 671
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 3 cut(s) 5, 256, 947
HpaII CCGG 2 cut(s) 86, 954
HphI GGTGA 4 cut(s) 73, 265, 521, 973
Hpy166II GTNNAC 1 cut(s) 436
Hpy188I TCNGA 5 cut(s) 154, 415, 587, 679, 1024
Hpy188III TCNNGA 4 cut(s) 53, 100, 773, 929
Hpy8I GTNNAC 1 cut(s) 436
HpyAV CCTTC 6 cut(s) 84, 142, 286, 565, 971, 1019
HpyCH4V TGCA 5 cut(s) 119, 128, 228, 527, 706
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 32, 125, 211, 949
HpyF3I CTNAG 6 cut(s) 78, 153, 584, 633, 665, 998
Hsp92II CATG 4 cut(s) 263, 562, 776, 1040
HspAI GCGC 2 cut(s) 35, 671
KpnI GGTACC 2 cut(s) 86, 758
Kzo9I GATC 7 cut(s) 66, 169, 391, 460, 732, 759, 876
LmnI GCTCC 2 cut(s) 202, 532
Lsp1109I GCAGC 1 cut(s) 140
LweI GCATC 4 cut(s) 115, 478, 565, 766
MaeIII GTNAC 4 cut(s) 277, 718, 853, 979
MalI GATC 7 cut(s) 68, 171, 393, 462, 734, 761, 878
MboI GATC 7 cut(s) 66, 169, 391, 460, 732, 759, 876
MboII GAAGA 7 cut(s) 76, 141, 341, 346, 419, 722, 1042
MflI RGATCY 1 cut(s) 876
MhlI GDGCHC 3 cut(s) 195, 641, 945
MlsI TGGCCA 2 cut(s) 469, 695
MluCI AATT 4 cut(s) 91, 342, 768, 918
MluNI TGGCCA 2 cut(s) 469, 695
MlyI GAGTC 1 cut(s) 956
MmeI TCCRAC 3 cut(s) 631, 702, 775
MnlI CCTC 8 cut(s) 148, 193, 523, 593, 621, 642, 912, 936
Mox20I TGGCCA 2 cut(s) 469, 695
MscI TGGCCA 2 cut(s) 469, 695
MslI CAYNNNNRTG 4 cut(s) 563, 701, 909, 1041
Msp20I TGGCCA 2 cut(s) 469, 695
MspI CCGG 2 cut(s) 86, 954
MspR9I CCNGG 3 cut(s) 221, 386, 954
MvaI CCWGG 2 cut(s) 221, 386
MwoI GCNNNNNNNGC 5 cut(s) 23, 32, 125, 211, 949
NciI CCSGG 1 cut(s) 954
NdeII GATC 7 cut(s) 66, 169, 391, 460, 732, 759, 876
NlaIII CATG 4 cut(s) 263, 562, 776, 1040
NlaIV GGNNCC 6 cut(s) 84, 159, 194, 688, 756, 958
NmuCI GTSAC 4 cut(s) 277, 718, 853, 979
NsbI TGCGCA 1 cut(s) 672
NspI RCATGY 1 cut(s) 1040
PaeR7I CTCGAG 1 cut(s) 944
PagI TCATGA 1 cut(s) 772
PfeI GAWTC 2 cut(s) 5, 256
PflMI CCANNNNNTGG 1 cut(s) 601
PkrI GCNGC 2 cut(s) 130, 301
PleI GAGTC 1 cut(s) 955
PpsI GAGTC 1 cut(s) 955
PpuMI RGGWCCY 1 cut(s) 687
PshAI GACNNNNGTC 1 cut(s) 1037
Psp5II RGGWCCY 1 cut(s) 687
Psp6I CCWGG 2 cut(s) 219, 384
PspGI CCWGG 2 cut(s) 219, 384
PspN4I GGNNCC 6 cut(s) 84, 159, 194, 688, 756, 958
PspPI GGNCC 3 cut(s) 217, 604, 687
PspPPI RGGWCCY 1 cut(s) 687
PspXI VCTCGAGB 1 cut(s) 944
PsuI RGATCY 1 cut(s) 876
RsaI GTAC 4 cut(s) 84, 449, 756, 971
RsaNI GTAC 4 cut(s) 83, 448, 755, 970
RseI CAYNNNNRTG 4 cut(s) 563, 701, 909, 1041
SatI GCNGC 2 cut(s) 129, 300
Sau3AI GATC 7 cut(s) 66, 169, 391, 460, 732, 759, 876
Sau96I GGNCC 3 cut(s) 217, 604, 687
SchI GAGTC 1 cut(s) 956
ScrFI CCNGG 3 cut(s) 221, 386, 954
SduI GDGCHC 3 cut(s) 195, 641, 945
SfaNI GCATC 4 cut(s) 115, 478, 565, 766
SfcI CTRYAG 1 cut(s) 728
Sfr274I CTCGAG 1 cut(s) 944
SinI GGWCC 3 cut(s) 217, 604, 687
SlaI CTCGAG 1 cut(s) 944
SmiMI CAYNNNNRTG 4 cut(s) 563, 701, 909, 1041
SmlI CTYRAG 1 cut(s) 944
SmoI CTYRAG 1 cut(s) 944
Sse9I AATT 4 cut(s) 91, 342, 768, 918
SsiI CCGC 2 cut(s) 299, 302
StyD4I CCNGG 3 cut(s) 219, 384, 952
StyI CCWWGG 3 cut(s) 690, 1008, 1053
TaqI TCGA 4 cut(s) 69, 459, 945, 1059
TasI AATT 4 cut(s) 91, 342, 768, 918
TatI WGTACW 2 cut(s) 447, 969
TauI GCSGC 1 cut(s) 302
TfiI GAWTC 2 cut(s) 5, 256
TscAI CASTG 2 cut(s) 641, 1048
TseFI GTSAC 4 cut(s) 277, 718, 853, 979
TseI GCWGC 1 cut(s) 128
Tsp45I GTSAC 4 cut(s) 277, 718, 853, 979
TspDTI ATGAA 3 cut(s) 120, 761, 1031
TspRI CASTG 2 cut(s) 641, 1048
Van91I CCANNNNNTGG 1 cut(s) 601
VpaK11BI GGWCC 3 cut(s) 217, 604, 687
XceI RCATGY 1 cut(s) 1040
XhoI CTCGAG 1 cut(s) 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.