Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 644 |
| AccB1I | GGYRCC | 1 cut(s) | 713 |
| AciI | CCGC | 1 cut(s) | 704 |
| AclWI | GGATC | 3 cut(s) | 70, 234, 556 |
| AcoI | YGGCCR | 1 cut(s) | 488 |
| AcsI | RAATTY | 1 cut(s) | 351 |
| AcuI | CTGAAG | 1 cut(s) | 432 |
| AfaI | GTAC | 5 cut(s) | 89, 684, 726, 857, 1087 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 429, 471, 501 |
| AflIII | ACRYGT | 1 cut(s) | 931 |
| AgsI | TTSAA | 6 cut(s) | 47, 326, 511, 869, 1028, 1036 |
| AhlI | ACTAGT | 1 cut(s) | 140 |
| AluBI | AGCT | 3 cut(s) | 214, 259, 878 |
| AluI | AGCT | 3 cut(s) | 214, 259, 878 |
| Alw21I | GWGCWC | 1 cut(s) | 700 |
| Alw44I | GTGCAC | 1 cut(s) | 696 |
| AlwI | GGATC | 3 cut(s) | 70, 234, 556 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 967 |
| Ama87I | CYCGRG | 1 cut(s) | 80 |
| AoxI | GGCC | 1 cut(s) | 488 |
| ApaLI | GTGCAC | 1 cut(s) | 696 |
| ApeKI | GCWGC | 2 cut(s) | 632, 635 |
| ApoI | RAATTY | 1 cut(s) | 351 |
| AseI | ATTAAT | 1 cut(s) | 71 |
| AspLEI | GCGC | 1 cut(s) | 645 |
| AspS9I | GGNCC | 2 cut(s) | 432, 981 |
| AsuII | TTCGAA | 1 cut(s) | 763 |
| AvaI | CYCGRG | 1 cut(s) | 80 |
| AvaII | GGWCC | 2 cut(s) | 432, 981 |
| BaeGI | GKGCMC | 2 cut(s) | 204, 700 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 956, 989 |
| BalI | TGGCCA | 1 cut(s) | 490 |
| BanI | GGYRCC | 1 cut(s) | 713 |
| BauI | CACGAG | 2 cut(s) | 203, 378 |
| Bbv12I | GWGCWC | 1 cut(s) | 700 |
| BbvI | GCAGC | 2 cut(s) | 644, 647 |
| BccI | CCATC | 4 cut(s) | 179, 299, 469, 586 |
| BceAI | ACGGC | 1 cut(s) | 937 |
| BcuI | ACTAGT | 1 cut(s) | 140 |
| BfaI | CTAG | 3 cut(s) | 141, 534, 686 |
| BglII | AGATCT | 1 cut(s) | 569 |
| BisI | GCNGC | 2 cut(s) | 633, 636 |
| BlsI | GCNGC | 2 cut(s) | 634, 637 |
| BmcAI | AGTACT | 2 cut(s) | 684, 1087 |
| Bme18I | GGWCC | 2 cut(s) | 432, 981 |
| BmeT110I | CYCGRG | 1 cut(s) | 80 |
| BmgT120I | GGNCC | 2 cut(s) | 432, 981 |
| BmiI | GGNNCC | 4 cut(s) | 36, 476, 715, 982 |
| BmsI | GCATC | 5 cut(s) | 29, 628, 697, 817, 923 |
| BpmI | CTGGAG | 1 cut(s) | 989 |
| Bpu14I | TTCGAA | 1 cut(s) | 763 |
| BpuEI | CTTGAG | 3 cut(s) | 178, 500, 854 |
| BsaJI | CCNNGG | 1 cut(s) | 500 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 429, 471, 501 |
| Bse1I | ACTGG | 6 cut(s) | 29, 57, 228, 434, 447, 972 |
| Bse3DI | GCAATG | 1 cut(s) | 638 |
| BseDI | CCNNGG | 1 cut(s) | 500 |
| BseGI | GGATG | 7 cut(s) | 174, 310, 461, 597, 607, 712, 909 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 429, 471, 501 |
| BseMI | GCAATG | 1 cut(s) | 638 |
| BseNI | ACTGG | 6 cut(s) | 29, 57, 228, 434, 447, 972 |
| BseRI | GAGGAG | 3 cut(s) | 221, 224, 572 |
| BseSI | GKGCMC | 2 cut(s) | 204, 700 |
| BseXI | GCAGC | 2 cut(s) | 644, 647 |
| BshFI | GGCC | 1 cut(s) | 490 |
| BshNI | GGYRCC | 1 cut(s) | 713 |
| BsiHKAI | GWGCWC | 1 cut(s) | 700 |
| BsiHKCI | CYCGRG | 1 cut(s) | 80 |
| BslFI | GGGAC | 1 cut(s) | 994 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 429, 471, 501 |
| BsmFI | GGGAC | 1 cut(s) | 994 |
| BsnI | GGCC | 1 cut(s) | 490 |
| BsoBI | CYCGRG | 1 cut(s) | 80 |
| Bsp119I | TTCGAA | 1 cut(s) | 763 |
| Bsp1286I | GDGCHC | 2 cut(s) | 204, 700 |
| Bsp143I | GATC | 6 cut(s) | 75, 106, 226, 561, 569, 958 |
| BspACI | CCGC | 1 cut(s) | 704 |
| BspANI | GGCC | 1 cut(s) | 490 |
| BspHI | TCATGA | 1 cut(s) | 145 |
| BspLI | GGNNCC | 4 cut(s) | 36, 476, 715, 982 |
| BspPI | GGATC | 3 cut(s) | 70, 234, 556 |
| BspQI | GCTCTTC | 1 cut(s) | 408 |
| BspT104I | TTCGAA | 1 cut(s) | 763 |
| BspT107I | GGYRCC | 1 cut(s) | 713 |
| BsrDI | GCAATG | 1 cut(s) | 638 |
| BsrI | ACTGG | 6 cut(s) | 29, 57, 228, 434, 447, 972 |
| BssECI | CCNNGG | 1 cut(s) | 500 |
| BssMI | GATC | 6 cut(s) | 75, 106, 226, 561, 569, 958 |
| BssSI | CACGAG | 2 cut(s) | 203, 378 |
| BssT1I | CCWWGG | 1 cut(s) | 500 |
| Bst2BI | CACGAG | 2 cut(s) | 203, 378 |
| Bst4CI | ACNGT | 1 cut(s) | 724 |
| Bst6I | CTCTTC | 3 cut(s) | 333, 408, 1031 |
| BstBI | TTCGAA | 1 cut(s) | 763 |
| BstC8I | GCNNGC | 2 cut(s) | 235, 712 |
| BstDEI | CTNAG | 2 cut(s) | 566, 692 |
| BstF5I | GGATG | 7 cut(s) | 174, 310, 461, 597, 607, 712, 909 |
| BstHHI | GCGC | 1 cut(s) | 645 |
| BstKTI | GATC | 6 cut(s) | 78, 109, 229, 564, 572, 961 |
| BstMBI | GATC | 6 cut(s) | 75, 106, 226, 561, 569, 958 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 524 |
| BstNSI | RCATGY | 2 cut(s) | 704, 935 |
| BstSLI | GKGCMC | 2 cut(s) | 204, 700 |
| BstV1I | GCAGC | 2 cut(s) | 644, 647 |
| BstX2I | RGATCY | 3 cut(s) | 226, 561, 569 |
| BstYI | RGATCY | 3 cut(s) | 226, 561, 569 |
| BsuRI | GGCC | 1 cut(s) | 490 |
| BtsCI | GGATG | 7 cut(s) | 174, 310, 461, 597, 607, 712, 909 |
| BtsIMutI | CAGTG | 2 cut(s) | 36, 440 |
| Cac8I | GCNNGC | 2 cut(s) | 235, 712 |
| CaiI | CAGNNNCTG | 1 cut(s) | 967 |
| CciI | TCATGA | 1 cut(s) | 145 |
| CfoI | GCGC | 1 cut(s) | 645 |
| Cfr13I | GGNCC | 2 cut(s) | 432, 981 |
| CseI | GACGC | 1 cut(s) | 446 |
| Csp6I | GTAC | 5 cut(s) | 88, 683, 725, 856, 1086 |
| CviAII | CATG | 7 cut(s) | 146, 269, 298, 358, 663, 701, 932 |
| CviQI | GTAC | 5 cut(s) | 88, 683, 725, 856, 1086 |
| DdeI | CTNAG | 2 cut(s) | 566, 692 |
| DpnI | GATC | 6 cut(s) | 77, 108, 228, 563, 571, 960 |
| DpnII | GATC | 6 cut(s) | 75, 106, 226, 561, 569, 958 |
| DraI | TTTAAA | 1 cut(s) | 96 |
| EaeI | YGGCCR | 1 cut(s) | 488 |
| Eam1104I | CTCTTC | 3 cut(s) | 333, 408, 1031 |
| EarI | CTCTTC | 3 cut(s) | 333, 408, 1031 |
| Eco130I | CCWWGG | 1 cut(s) | 500 |
| Eco32I | GATATC | 1 cut(s) | 166 |
| Eco47I | GGWCC | 2 cut(s) | 432, 981 |
| Eco57I | CTGAAG | 1 cut(s) | 432 |
| Eco88I | CYCGRG | 1 cut(s) | 80 |
| EcoRV | GATATC | 1 cut(s) | 166 |
| EcoT14I | CCWWGG | 1 cut(s) | 500 |
| EcoT22I | ATGCAT | 2 cut(s) | 70, 916 |
| ErhI | CCWWGG | 1 cut(s) | 500 |
| FaeI | CATG | 7 cut(s) | 149, 272, 301, 361, 666, 704, 935 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 892, 924 |
| FaqI | GGGAC | 1 cut(s) | 994 |
| FatI | CATG | 7 cut(s) | 145, 268, 297, 357, 662, 700, 931 |
| FauNDI | CATATG | 1 cut(s) | 529 |
| Fnu4HI | GCNGC | 2 cut(s) | 633, 636 |
| FokI | GGATG | 7 cut(s) | 161, 317, 448, 604, 614, 719, 916 |
| Fsp4HI | GCNGC | 2 cut(s) | 633, 636 |
| FspBI | CTAG | 3 cut(s) | 141, 534, 686 |
| FspI | TGCGCA | 1 cut(s) | 644 |
| GlaI | GCGC | 1 cut(s) | 644 |
| GluI | GCNGC | 2 cut(s) | 633, 636 |
| GsuI | CTGGAG | 1 cut(s) | 989 |
| HaeIII | GGCC | 1 cut(s) | 490 |
| HgaI | GACGC | 1 cut(s) | 446 |
| HhaI | GCGC | 1 cut(s) | 645 |
| Hin1II | CATG | 7 cut(s) | 149, 272, 301, 361, 666, 704, 935 |
| Hin6I | GCGC | 1 cut(s) | 643 |
| HinP1I | GCGC | 1 cut(s) | 643 |
| HincII | GTYRAC | 1 cut(s) | 363 |
| HindII | GTYRAC | 1 cut(s) | 363 |
| HinfI | GANTC | 6 cut(s) | 249, 507, 760, 796, 971, 1024 |
| Hpy166II | GTNNAC | 2 cut(s) | 363, 698 |
| Hpy188I | TCNGA | 5 cut(s) | 160, 436, 752, 963, 990 |
| Hpy188III | TCNNGA | 5 cut(s) | 11, 47, 146, 623, 833 |
| Hpy8I | GTNNAC | 2 cut(s) | 363, 698 |
| Hpy99I | CGWCG | 1 cut(s) | 440 |
| HpyAV | CCTTC | 5 cut(s) | 295, 377, 592, 994, 1048 |
| HpyCH4III | ACNGT | 1 cut(s) | 724 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 524 |
| HpyF3I | CTNAG | 2 cut(s) | 566, 692 |
| Hsp92II | CATG | 7 cut(s) | 149, 272, 301, 361, 666, 704, 935 |
| HspAI | GCGC | 1 cut(s) | 643 |
| Kzo9I | GATC | 6 cut(s) | 75, 106, 226, 561, 569, 958 |
| LguI | GCTCTTC | 1 cut(s) | 408 |
| LmnI | GCTCC | 2 cut(s) | 211, 843 |
| Lsp1109I | GCAGC | 2 cut(s) | 644, 647 |
| LweI | GCATC | 5 cut(s) | 29, 628, 697, 817, 923 |
| MaeI | CTAG | 3 cut(s) | 141, 534, 686 |
| MaeIII | GTNAC | 2 cut(s) | 406, 880 |
| MalI | GATC | 6 cut(s) | 77, 108, 228, 563, 571, 960 |
| MboI | GATC | 6 cut(s) | 75, 106, 226, 561, 569, 958 |
| MboII | GAAGA | 8 cut(s) | 147, 244, 350, 355, 425, 599, 749, 1048 |
| MflI | RGATCY | 3 cut(s) | 226, 561, 569 |
| MhlI | GDGCHC | 2 cut(s) | 204, 700 |
| MlsI | TGGCCA | 1 cut(s) | 490 |
| MluCI | AATT | 3 cut(s) | 351, 945, 1076 |
| MluNI | TGGCCA | 1 cut(s) | 490 |
| MlyI | GAGTC | 1 cut(s) | 980 |
| MmeI | TCCRAC | 3 cut(s) | 459, 532, 802 |
| MnlI | CCTC | 9 cut(s) | 199, 202, 434, 508, 517, 550, 669, 1032, 1042 |
| Mox20I | TGGCCA | 1 cut(s) | 490 |
| Mph1103I | ATGCAT | 2 cut(s) | 70, 916 |
| MscI | TGGCCA | 1 cut(s) | 490 |
| MseI | TTAA | 7 cut(s) | 71, 95, 216, 264, 543, 581, 1090 |
| MslI | CAYNNNNRTG | 3 cut(s) | 705, 936, 989 |
| Msp20I | TGGCCA | 1 cut(s) | 490 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 524 |
| NdeI | CATATG | 1 cut(s) | 529 |
| NdeII | GATC | 6 cut(s) | 75, 106, 226, 561, 569, 958 |
| NlaIII | CATG | 7 cut(s) | 149, 272, 301, 361, 666, 704, 935 |
| NlaIV | GGNNCC | 4 cut(s) | 36, 476, 715, 982 |
| NmuCI | GTSAC | 1 cut(s) | 880 |
| NsbI | TGCGCA | 1 cut(s) | 644 |
| NsiI | ATGCAT | 2 cut(s) | 70, 916 |
| NspI | RCATGY | 2 cut(s) | 704, 935 |
| NspV | TTCGAA | 1 cut(s) | 763 |
| PagI | TCATGA | 1 cut(s) | 145 |
| PciI | ACATGT | 1 cut(s) | 931 |
| PciSI | GCTCTTC | 1 cut(s) | 408 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 432 |
| PfeI | GAWTC | 5 cut(s) | 249, 507, 760, 796, 1024 |
| PkrI | GCNGC | 2 cut(s) | 634, 637 |
| PleI | GAGTC | 1 cut(s) | 979 |
| PpsI | GAGTC | 1 cut(s) | 979 |
| PscI | ACATGT | 1 cut(s) | 931 |
| PshBI | ATTAAT | 1 cut(s) | 71 |
| PspN4I | GGNNCC | 4 cut(s) | 36, 476, 715, 982 |
| PspPI | GGNCC | 2 cut(s) | 432, 981 |
| PstNI | CAGNNNCTG | 1 cut(s) | 967 |
| PsuI | RGATCY | 3 cut(s) | 226, 561, 569 |
| RsaI | GTAC | 5 cut(s) | 89, 684, 726, 857, 1087 |
| RsaNI | GTAC | 5 cut(s) | 88, 683, 725, 856, 1086 |
| RseI | CAYNNNNRTG | 3 cut(s) | 705, 936, 989 |
| SapI | GCTCTTC | 1 cut(s) | 408 |
| SaqAI | TTAA | 7 cut(s) | 71, 95, 216, 264, 543, 581, 1090 |
| SatI | GCNGC | 2 cut(s) | 633, 636 |
| Sau3AI | GATC | 6 cut(s) | 75, 106, 226, 561, 569, 958 |
| Sau96I | GGNCC | 2 cut(s) | 432, 981 |
| ScaI | AGTACT | 2 cut(s) | 684, 1087 |
| SchI | GAGTC | 1 cut(s) | 980 |
| SduI | GDGCHC | 2 cut(s) | 204, 700 |
| SetI | ASST | 7 cut(s) | 93, 216, 261, 480, 691, 880, 1005 |
| SfaNI | GCATC | 5 cut(s) | 29, 628, 697, 817, 923 |
| SfuI | TTCGAA | 1 cut(s) | 763 |
| SinI | GGWCC | 2 cut(s) | 432, 981 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 705, 936, 989 |
| SmlI | CTYRAG | 3 cut(s) | 193, 479, 833 |
| SmoI | CTYRAG | 3 cut(s) | 193, 479, 833 |
| SpeI | ACTAGT | 1 cut(s) | 140 |
| Sse9I | AATT | 3 cut(s) | 351, 945, 1076 |
| SsiI | CCGC | 1 cut(s) | 704 |
| SspMI | CTAG | 3 cut(s) | 141, 534, 686 |
| StyI | CCWWGG | 1 cut(s) | 500 |
| TaaI | ACNGT | 1 cut(s) | 724 |
| TaqI | TCGA | 4 cut(s) | 426, 763, 936, 957 |
| TasI | AATT | 3 cut(s) | 351, 945, 1076 |
| TatI | WGTACW | 4 cut(s) | 682, 724, 855, 1085 |
| TfiI | GAWTC | 5 cut(s) | 249, 507, 760, 796, 1024 |
| Tru1I | TTAA | 7 cut(s) | 71, 95, 216, 264, 543, 581, 1090 |
| Tru9I | TTAA | 7 cut(s) | 71, 95, 216, 264, 543, 581, 1090 |
| TscAI | CASTG | 2 cut(s) | 36, 447 |
| TseFI | GTSAC | 1 cut(s) | 880 |
| TseI | GCWGC | 2 cut(s) | 632, 635 |
| Tsp45I | GTSAC | 1 cut(s) | 880 |
| TspDTI | ATGAA | 1 cut(s) | 314 |
| TspRI | CASTG | 2 cut(s) | 36, 447 |
| VneI | GTGCAC | 1 cut(s) | 696 |
| VpaK11BI | GGWCC | 2 cut(s) | 432, 981 |
| VspI | ATTAAT | 1 cut(s) | 71 |
| XapI | RAATTY | 1 cut(s) | 351 |
| XceI | RCATGY | 2 cut(s) | 704, 935 |
| XspI | CTAG | 3 cut(s) | 141, 534, 686 |
| ZrmI | AGTACT | 2 cut(s) | 684, 1087 |
| Zsp2I | ATGCAT | 2 cut(s) | 70, 916 |