Rw6G001500

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000451
Physical Location & Seq
Forward (+)
2357159 .. 2361237
4079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G001500.1

Sequence Viewer

Length: 1047 bp
ATGAGTTTAAAGGCACCAAATGTACAGGACATGGTGAGGAGTGACCCTTTTCACATCCCTGATTCATTCCTTGCAGTCAGGAATGAAGAAGACGAGCCAAAGAGTGCAGATGCATCTCGTGATCTCTGTTCTGAGATTCCTATCATTGATTTTACTCTGCTTTCGAAAGGGCAGAAGGAGGAGCTTAACAAACTGGACCTGGCTTGCAAAGAATGGGGATTCTTTCAGGTGGTAAACCATGGAGTAGAAACACATGTGTTGCAGGGAATGAAGGATGCTGCTGCGAAATTCTTTGACCTTCCATTAGAAGAGAAGAGCAAAATTAGTATGTCACTGGATGAGTTTCAAGGCTACGGGCAAGCCTATGCTGCTGCCGAAGGGCAGACCCTTGACTGGTCTGACACACTGTTTCTCAGTGTTTATCCATCTCATAGTAGAAACCTTGAGTTTTGGCCAACATCACCAAAAGGATTCAAGGAAGCAATTGAGGCATATTCTACTGAAGTTAAAAGGATTGGAGAGGAGCTCTTAGGGTCTATCTCTCAAATTATGGGGATGCAGAAGGATGCTCTTCTTGGATTTCATAAAGAGTTGGTCCAAGTTTTTCGTGTGACCTACTATCCTCCGTGCTCCAAGCCAGATAAAGTACTAGGCTTAAGTCCACACTCCGACACAAGCACCATAACCATACTCATGCAGGAAGACGATGTTACCGGATTACATATTCGAAAAGAAGCAAAATGGGTGCCGGTAGAGCCAATTCCAAATGCTTTCGTTGTGAATGTCGGAGATATGATTGAGATATGGAGTAATGGGAAGTACAAGAGCATTGAACATAGAGCTGTGACGAACAAAAGCAAGGCAAGATTATCTTATGCAACATTTATAATTCCACGTAACGATGTTGAAATTGGACCATTTAATCATTTAGTGGACCGGTCATCTCGAAAGTACAAGAAAGTCGTATACGGAGAATATTTGAGGAGTTCCTTGAAGGGAAAACTTGAGGGGAAGTCGCACACTGAAACAGCAAAGATCGGAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N Thaumatin

Protein Analysis

348

Amino Acids

39.18

Weight (kDa)

5.97

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 46 - 153 4.3e-30 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 200 - 297 8e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000451)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13280 FvH4_6g13281 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13300 FvH4_6g13322
malus_domestica MD04G1132000.v1.1 MD04G1132100.v1.1 MD04G1132400.v1.1 MD12G1145100.v1.1 MD12G1145200.v1.1
prunus_persica Prupe.6G258600_v2.0.a1 Prupe.6G258700_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258900_v2.0.a1
pyrus_communis pycom04g12010 pycom04g12020
rosa_chinensis RchiOBHm_Chr3g0466131 RchiOBHm_Chr3g0466151 RchiOBHm_Chr3g0466161 RchiOBHm_Chr3g0466171 RchiOBHm_Chr3g0466181 RchiOBHm_Chr3g0466201 RchiOBHm_Chr3g0466211 RchiOBHm_Chr6g0251841
rosa_laevigata RLG00000015425 RLG00000024596 RLG00000024599 RLG00000024600 RLG00000024601 RLG00000024602
rosa_multiflora Rmu_co8112718.1_g000001 Rmu_co8178858.1_g000001 Rmu_co8388385.1_g000001 Rmu_sc0000350.1_g000002 Rmu_sc0000350.1_g000003 Rmu_sc0000350.1_g000004 Rmu_sc0000350.1_g000006 Rmu_sc0000350.1_g000011 Rmu_sc0002249.1_g000004 Rmu_sc0007597.1_g000012 Rmu_sc0012177.1_g000002 Rmu_sc0012177.1_g000006 Rmu_sc0012177.1_g000007 Rmu_sc0022084.1_g000003 Rmu_ssc0000303.1_g000004
rosa_roxburghii Rroxscaffold_6G00414720 Rroxscaffold_6G00414730 Rroxscaffold_6G00414740 Rroxscaffold_6G00414750 Rroxscaffold_6G00414760 Rroxscaffold_6G00414770 Rroxscaffold_6G00414780 Rroxscaffold_6G00414790
rosa_rugosa Rorug03G0079600 Rorug03G0079600 Rorug03G0079800 Rorug03G0080100 Rorug03G0080200 Rorug03G0080300 Rorug03G0080400 Rorug05G0504900
rosa_samantha Rh3BG147200 Rh3BG147300 Rh3BG147400 Rh3BG147500 Rh3BG147600 Rh3CG147900 Rh3CG148000 Rh3CG148100 Rh3CG148200 Rh3CG148300 Rh3CG148500 Rh3CG148600 Rh3DG147800 Rh3DG147900 Rh3DG148100 Rh3DG148300 Rh4BG237100 Rh6AG015600 Rh6AG015700 Rh6BG013700 Rh6CG069500
rosa_wichuraiana Rw3G011870 Rw3G011880 Rw3G011890 Rw3G011900 Rw3G011910 Rw3G011930 Rw6G001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 887
AccB1I GGYRCC 2 cut(s) 13, 745
AccI GTMKAC 1 cut(s) 966
AcoI YGGCCR 1 cut(s) 452
AcsI RAATTY 1 cut(s) 287
AcuI CTGAAG 1 cut(s) 522
AfaI GTAC 4 cut(s) 24, 648, 821, 953
AfiI CCNNNNNNNGG 1 cut(s) 393
AflII CTTAAG 1 cut(s) 655
AflIII ACRYGT 1 cut(s) 253
AgeI ACCGGT 1 cut(s) 936
AgsI TTSAA 5 cut(s) 347, 475, 833, 908, 994
AjnI CCWGG 1 cut(s) 198
AluBI AGCT 3 cut(s) 184, 526, 842
AluI AGCT 3 cut(s) 184, 526, 842
Alw21I GWGCWC 2 cut(s) 528, 632
AoxI GGCC 1 cut(s) 452
ApeKI GCWGC 4 cut(s) 278, 281, 368, 371
ApoI RAATTY 1 cut(s) 287
AsiGI ACCGGT 1 cut(s) 936
AspS9I GGNCC 4 cut(s) 196, 595, 914, 934
AsuHPI GGTGA 2 cut(s) 46, 453
AsuII TTCGAA 2 cut(s) 164, 727
AvaII GGWCC 4 cut(s) 196, 595, 914, 934
BalI TGGCCA 1 cut(s) 454
BanI GGYRCC 2 cut(s) 13, 745
BanII GRGCYC 1 cut(s) 528
BauI CACGAG 1 cut(s) 117
BbsI GAAGAC 2 cut(s) 96, 708
Bbv12I GWGCWC 2 cut(s) 528, 632
BbvI GCAGC 4 cut(s) 265, 268, 355, 358
BccI CCATC 1 cut(s) 433
BciT130I CCWGG 1 cut(s) 200
BfaI CTAG 1 cut(s) 650
BfrI CTTAAG 1 cut(s) 655
BisI GCNGC 4 cut(s) 279, 282, 369, 372
BlsI GCNGC 4 cut(s) 280, 283, 370, 373
BmcAI AGTACT 1 cut(s) 648
Bme1390I CCNGG 1 cut(s) 200
Bme18I GGWCC 4 cut(s) 196, 595, 914, 934
BmgT120I GGNCC 4 cut(s) 196, 595, 914, 934
BmiI GGNNCC 2 cut(s) 15, 747
BmrFI CCNGG 1 cut(s) 200
BmsI GCATC 5 cut(s) 100, 122, 265, 546, 556
BpiI GAAGAC 2 cut(s) 96, 708
BplI GAGNNNNNCTC 2 cut(s) 510, 542
Bpu14I TTCGAA 2 cut(s) 164, 727
BpuEI CTTGAG 2 cut(s) 464, 1025
BsaAI YACGTR 1 cut(s) 896
BsaBI GATNNNNATC 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 238
BsaWI WCCGGW 2 cut(s) 713, 936
Bsc4I CCNNNNNNNGG 1 cut(s) 393
Bse118I RCCGGY 2 cut(s) 748, 936
Bse1I ACTGG 3 cut(s) 198, 339, 398
Bse8I GATNNNNATC 1 cut(s) 140
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 1 cut(s) 238
BseGI GGATG 5 cut(s) 54, 280, 343, 561, 571
BseJI GATNNNNATC 1 cut(s) 140
BseLI CCNNNNNNNGG 1 cut(s) 393
BseMII CTCAG 2 cut(s) 123, 427
BseNI ACTGG 3 cut(s) 198, 339, 398
BseRI GAGGAG 4 cut(s) 52, 194, 536, 997
BseXI GCAGC 4 cut(s) 265, 268, 355, 358
BsgI GTGCAG 1 cut(s) 126
BshFI GGCC 1 cut(s) 454
BshNI GGYRCC 2 cut(s) 13, 745
BshTI ACCGGT 1 cut(s) 936
BsiHKAI GWGCWC 2 cut(s) 528, 632
BsiSI CCGG 3 cut(s) 714, 749, 937
BslI CCNNNNNNNGG 1 cut(s) 393
BsnI GGCC 1 cut(s) 454
Bsp119I TTCGAA 2 cut(s) 164, 727
Bsp1286I GDGCHC 2 cut(s) 528, 632
Bsp1407I TGTACA 1 cut(s) 22
Bsp143I GATC 2 cut(s) 121, 1035
Bsp19I CCATGG 1 cut(s) 238
BspANI GGCC 1 cut(s) 454
BspCNI CTCAG 2 cut(s) 124, 426
BspLI GGNNCC 2 cut(s) 15, 747
BspQI GCTCTTC 2 cut(s) 308, 576
BspT104I TTCGAA 2 cut(s) 164, 727
BspT107I GGYRCC 2 cut(s) 13, 745
BspTI CTTAAG 1 cut(s) 655
BsrFI RCCGGY 2 cut(s) 748, 936
BsrGI TGTACA 1 cut(s) 22
BsrI ACTGG 3 cut(s) 198, 339, 398
BssAI RCCGGY 2 cut(s) 748, 936
BssECI CCNNGG 1 cut(s) 238
BssMI GATC 2 cut(s) 121, 1035
BssNAI GTATAC 1 cut(s) 967
BssSI CACGAG 1 cut(s) 117
BssT1I CCWWGG 1 cut(s) 238
Bst1107I GTATAC 1 cut(s) 967
Bst2BI CACGAG 1 cut(s) 117
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 408
Bst6I CTCTTC 3 cut(s) 303, 308, 576
BstAFI CTTAAG 1 cut(s) 655
BstAUI TGTACA 1 cut(s) 22
BstBAI YACGTR 1 cut(s) 896
BstBI TTCGAA 2 cut(s) 164, 727
BstC8I GCNNGC 2 cut(s) 205, 360
BstDEI CTNAG 3 cut(s) 132, 413, 529
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 5 cut(s) 54, 280, 343, 561, 571
BstKTI GATC 2 cut(s) 124, 1038
BstMBI GATC 2 cut(s) 121, 1035
BstMWI GCNNNNNNNGC 3 cut(s) 368, 488, 754
BstNI CCWGG 1 cut(s) 200
BstNSI RCATGY 1 cut(s) 257
BstSCI CCNGG 1 cut(s) 198
BstV1I GCAGC 4 cut(s) 265, 268, 355, 358
BstV2I GAAGAC 2 cut(s) 96, 708
BstZ17I GTATAC 1 cut(s) 967
BsuRI GGCC 1 cut(s) 454
BtgI CCRYGG 1 cut(s) 238
BtsCI GGATG 5 cut(s) 54, 280, 343, 561, 571
BtsIMutI CAGTG 4 cut(s) 332, 404, 421, 1020
Cac8I GCNNGC 2 cut(s) 205, 360
Cfr10I RCCGGY 2 cut(s) 748, 936
Cfr13I GGNCC 4 cut(s) 196, 595, 914, 934
Csp6I GTAC 4 cut(s) 23, 647, 820, 952
CspAI ACCGGT 1 cut(s) 936
CviAII CATG 4 cut(s) 31, 239, 254, 694
CviQI GTAC 4 cut(s) 23, 647, 820, 952
DdeI CTNAG 3 cut(s) 132, 413, 529
DpnI GATC 2 cut(s) 123, 1037
DpnII GATC 2 cut(s) 121, 1035
DraI TTTAAA 1 cut(s) 9
EaeI YGGCCR 1 cut(s) 452
Eam1104I CTCTTC 3 cut(s) 303, 308, 576
EarI CTCTTC 3 cut(s) 303, 308, 576
Ecl136II GAGCTC 1 cut(s) 526
Eco130I CCWWGG 1 cut(s) 238
Eco24I GRGCYC 1 cut(s) 528
Eco47I GGWCC 4 cut(s) 196, 595, 914, 934
Eco53kI GAGCTC 1 cut(s) 526
Eco57I CTGAAG 1 cut(s) 522
EcoICRI GAGCTC 1 cut(s) 526
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 1 cut(s) 238
EcoT22I ATGCAT 1 cut(s) 115
EcoT38I GRGCYC 1 cut(s) 528
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 4 cut(s) 34, 242, 257, 697
FalI AAGNNNNNCTT 2 cut(s) 856, 888
FatI CATG 4 cut(s) 30, 238, 253, 693
FblI GTMKAC 1 cut(s) 966
Fnu4HI GCNGC 4 cut(s) 279, 282, 369, 372
FokI GGATG 5 cut(s) 41, 287, 350, 568, 578
FriOI GRGCYC 1 cut(s) 528
Fsp4HI GCNGC 4 cut(s) 279, 282, 369, 372
FspBI CTAG 1 cut(s) 650
GluI GCNGC 4 cut(s) 279, 282, 369, 372
HaeIII GGCC 1 cut(s) 454
HapII CCGG 3 cut(s) 714, 749, 937
Hin1II CATG 4 cut(s) 34, 242, 257, 697
HinfI GANTC 4 cut(s) 62, 136, 219, 471
HpaII CCGG 3 cut(s) 714, 749, 937
HphI GGTGA 2 cut(s) 46, 453
Hpy166II GTNNAC 4 cut(s) 235, 662, 934, 967
Hpy188I TCNGA 5 cut(s) 133, 400, 670, 788, 1040
Hpy188III TCNNGA 3 cut(s) 79, 119, 945
Hpy8I GTNNAC 4 cut(s) 235, 662, 934, 967
HpyAV CCTTC 6 cut(s) 169, 265, 308, 371, 556, 988
HpyCH4III ACNGT 1 cut(s) 408
HpyCH4IV ACGT 1 cut(s) 895
HpyCH4V TGCA 8 cut(s) 74, 107, 113, 207, 262, 559, 697, 878
HpyF10VI GCNNNNNNNGC 3 cut(s) 368, 488, 754
HpyF3I CTNAG 3 cut(s) 132, 413, 529
HpySE526I ACGT 1 cut(s) 895
Hsp92II CATG 4 cut(s) 34, 242, 257, 697
Kzo9I GATC 2 cut(s) 121, 1035
LguI GCTCTTC 2 cut(s) 308, 576
LmnI GCTCC 3 cut(s) 181, 523, 635
Lsp1109I GCAGC 4 cut(s) 265, 268, 355, 358
LweI GCATC 5 cut(s) 100, 122, 265, 546, 556
MaeI CTAG 1 cut(s) 650
MaeII ACGT 1 cut(s) 895
MaeIII GTNAC 6 cut(s) 41, 330, 610, 709, 844, 896
MalI GATC 2 cut(s) 123, 1037
MboI GATC 2 cut(s) 121, 1035
MboII GAAGA 6 cut(s) 98, 101, 320, 325, 563, 713
MfeI CAATTG 1 cut(s) 483
MhlI GDGCHC 2 cut(s) 528, 632
MlsI TGGCCA 1 cut(s) 454
MluCI AATT 7 cut(s) 287, 321, 483, 546, 759, 888, 909
MluNI TGGCCA 1 cut(s) 454
MmeI TCCRAC 2 cut(s) 693, 766
MnlI CCTC 7 cut(s) 30, 172, 481, 514, 633, 975, 1000
Mox20I TGGCCA 1 cut(s) 454
Mph1103I ATGCAT 1 cut(s) 115
MscI TGGCCA 1 cut(s) 454
MseI TTAA 6 cut(s) 8, 186, 507, 656, 921, 1045
MslI CAYNNNNRTG 1 cut(s) 692
Msp20I TGGCCA 1 cut(s) 454
MspCI CTTAAG 1 cut(s) 655
MspI CCGG 3 cut(s) 714, 749, 937
MspR9I CCNGG 1 cut(s) 200
MunI CAATTG 1 cut(s) 483
MvaI CCWGG 1 cut(s) 200
MwoI GCNNNNNNNGC 3 cut(s) 368, 488, 754
NcoI CCATGG 1 cut(s) 238
NdeII GATC 2 cut(s) 121, 1035
NlaIII CATG 4 cut(s) 34, 242, 257, 697
NlaIV GGNNCC 2 cut(s) 15, 747
NmuCI GTSAC 4 cut(s) 41, 330, 610, 844
NsiI ATGCAT 1 cut(s) 115
NspI RCATGY 1 cut(s) 257
NspV TTCGAA 2 cut(s) 164, 727
PciI ACATGT 1 cut(s) 253
PciSI GCTCTTC 2 cut(s) 308, 576
PfeI GAWTC 4 cut(s) 62, 136, 219, 471
PinAI ACCGGT 1 cut(s) 936
PkrI GCNGC 4 cut(s) 280, 283, 370, 373
Ppu21I YACGTR 1 cut(s) 896
PscI ACATGT 1 cut(s) 253
PsiI TTATAA 1 cut(s) 887
Psp124BI GAGCTC 1 cut(s) 528
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PspN4I GGNNCC 2 cut(s) 15, 747
PspPI GGNCC 4 cut(s) 196, 595, 914, 934
RsaI GTAC 4 cut(s) 24, 648, 821, 953
RsaNI GTAC 4 cut(s) 23, 647, 820, 952
RseI CAYNNNNRTG 1 cut(s) 692
SacI GAGCTC 1 cut(s) 528
SapI GCTCTTC 2 cut(s) 308, 576
SaqAI TTAA 6 cut(s) 8, 186, 507, 656, 921, 1045
SatI GCNGC 4 cut(s) 279, 282, 369, 372
Sau3AI GATC 2 cut(s) 121, 1035
Sau96I GGNCC 4 cut(s) 196, 595, 914, 934
ScaI AGTACT 1 cut(s) 648
ScrFI CCNGG 1 cut(s) 200
SduI GDGCHC 2 cut(s) 528, 632
SetI ASST 9 cut(s) 186, 201, 231, 300, 444, 528, 617, 844, 898
SfaNI GCATC 5 cut(s) 100, 122, 265, 546, 556
SfuI TTCGAA 2 cut(s) 164, 727
SinI GGWCC 4 cut(s) 196, 595, 914, 934
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 3 cut(s) 443, 655, 1004
SmoI CTYRAG 3 cut(s) 443, 655, 1004
Sse9I AATT 7 cut(s) 287, 321, 483, 546, 759, 888, 909
SspI AATATT 1 cut(s) 977
SspMI CTAG 1 cut(s) 650
SstI GAGCTC 1 cut(s) 528
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 1 cut(s) 408
TaiI ACGT 1 cut(s) 898
TaqI TCGA 3 cut(s) 164, 727, 946
TasI AATT 7 cut(s) 287, 321, 483, 546, 759, 888, 909
TatI WGTACW 4 cut(s) 22, 646, 819, 951
TfiI GAWTC 4 cut(s) 62, 136, 219, 471
Tru1I TTAA 6 cut(s) 8, 186, 507, 656, 921, 1045
Tru9I TTAA 6 cut(s) 8, 186, 507, 656, 921, 1045
TscAI CASTG 4 cut(s) 339, 411, 421, 1027
TseFI GTSAC 4 cut(s) 41, 330, 610, 844
TseI GCWGC 4 cut(s) 278, 281, 368, 371
Tsp45I GTSAC 4 cut(s) 41, 330, 610, 844
TspDTI ATGAA 4 cut(s) 54, 99, 284, 572
TspGWI ACGGA 2 cut(s) 615, 984
TspRI CASTG 4 cut(s) 339, 411, 421, 1027
Vha464I CTTAAG 1 cut(s) 655
VpaK11BI GGWCC 4 cut(s) 196, 595, 914, 934
XapI RAATTY 1 cut(s) 287
XceI RCATGY 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 966
XspI CTAG 1 cut(s) 650
ZrmI AGTACT 1 cut(s) 648
Zsp2I ATGCAT 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.