Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 2 cut(s) | 91, 811 |
| AccB1I | GGYRCC | 2 cut(s) | 91, 811 |
| AciI | CCGC | 2 cut(s) | 179, 353 |
| AclWI | GGATC | 1 cut(s) | 116 |
| AcoI | YGGCCR | 2 cut(s) | 86, 524 |
| AcsI | RAATTY | 4 cut(s) | 388, 477, 1079, 1103 |
| AfaI | GTAC | 5 cut(s) | 93, 714, 813, 887, 999 |
| AflIII | ACRYGT | 1 cut(s) | 577 |
| AgeI | ACCGGT | 1 cut(s) | 814 |
| AgsI | TTSAA | 4 cut(s) | 104, 368, 644, 1103 |
| AjnI | CCWGG | 1 cut(s) | 270 |
| AleI | CACNNNNGTG | 1 cut(s) | 17 |
| AluBI | AGCT | 6 cut(s) | 256, 410, 548, 592, 658, 743 |
| AluI | AGCT | 6 cut(s) | 256, 410, 548, 592, 658, 743 |
| Alw21I | GWGCWC | 2 cut(s) | 594, 903 |
| Alw26I | GTCTC | 1 cut(s) | 409 |
| AlwI | GGATC | 1 cut(s) | 116 |
| Ama87I | CYCGRG | 1 cut(s) | 485 |
| AoxI | GGCC | 2 cut(s) | 86, 524 |
| ApeKI | GCWGC | 1 cut(s) | 350 |
| ApoI | RAATTY | 4 cut(s) | 388, 477, 1079, 1103 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 347, 379 |
| AsiGI | ACCGGT | 1 cut(s) | 814 |
| Asp718I | GGTACC | 2 cut(s) | 91, 811 |
| AspLEI | GCGC | 1 cut(s) | 958 |
| AsuHPI | GGTGA | 1 cut(s) | 829 |
| AsuNHI | GCTAGC | 1 cut(s) | 743 |
| AvaI | CYCGRG | 1 cut(s) | 485 |
| AxyI | CCTNAGG | 1 cut(s) | 516 |
| BalI | TGGCCA | 1 cut(s) | 526 |
| BanI | GGYRCC | 2 cut(s) | 91, 811 |
| BanII | GRGCYC | 2 cut(s) | 594, 984 |
| BbsI | GAAGAC | 1 cut(s) | 168 |
| Bbv12I | GWGCWC | 2 cut(s) | 594, 903 |
| BbvI | GCAGC | 1 cut(s) | 337 |
| BccI | CCATC | 2 cut(s) | 412, 616 |
| BciT130I | CCWGG | 1 cut(s) | 272 |
| BclI | TGATCA | 1 cut(s) | 988 |
| BcoDI | GTCTC | 1 cut(s) | 409 |
| BfaI | CTAG | 2 cut(s) | 716, 744 |
| BglII | AGATCT | 1 cut(s) | 599 |
| BisI | GCNGC | 1 cut(s) | 351 |
| BlsI | GCNGC | 1 cut(s) | 352 |
| BmcAI | AGTACT | 1 cut(s) | 714 |
| Bme1390I | CCNGG | 1 cut(s) | 272 |
| BmeT110I | CYCGRG | 1 cut(s) | 485 |
| BmiI | GGNNCC | 3 cut(s) | 93, 135, 813 |
| BmrFI | CCNGG | 1 cut(s) | 272 |
| BmsI | GCATC | 5 cut(s) | 337, 348, 847, 953, 958 |
| BmtI | GCTAGC | 1 cut(s) | 747 |
| BoxI | GACNNNNGTC | 1 cut(s) | 771 |
| BpiI | GAAGAC | 1 cut(s) | 168 |
| BpmI | CTGGAG | 1 cut(s) | 993 |
| BpuEI | CTTGAG | 1 cut(s) | 524 |
| BsaBI | GATNNNNATC | 2 cut(s) | 212, 604 |
| BsaWI | WCCGGW | 2 cut(s) | 779, 814 |
| Bse118I | RCCGGY | 2 cut(s) | 88, 814 |
| Bse1I | ACTGG | 1 cut(s) | 114 |
| Bse21I | CCTNAGG | 1 cut(s) | 516 |
| Bse8I | GATNNNNATC | 2 cut(s) | 212, 604 |
| BseBI | CCWGG | 1 cut(s) | 272 |
| BseGI | GGATG | 4 cut(s) | 352, 627, 637, 888 |
| BseJI | GATNNNNATC | 2 cut(s) | 212, 604 |
| BseMII | CTCAG | 5 cut(s) | 195, 425, 438, 507, 587 |
| BseNI | ACTGG | 1 cut(s) | 114 |
| BseRI | GAGGAG | 3 cut(s) | 266, 464, 602 |
| BseX3I | CGGCCG | 1 cut(s) | 86 |
| BseXI | GCAGC | 1 cut(s) | 337 |
| BsgI | GTGCAG | 1 cut(s) | 50 |
| Bsh1285I | CGRYCG | 1 cut(s) | 89 |
| BshFI | GGCC | 2 cut(s) | 88, 526 |
| BshNI | GGYRCC | 2 cut(s) | 91, 811 |
| BshTI | ACCGGT | 1 cut(s) | 814 |
| BsiEI | CGRYCG | 1 cut(s) | 89 |
| BsiHKAI | GWGCWC | 2 cut(s) | 594, 903 |
| BsiHKCI | CYCGRG | 1 cut(s) | 485 |
| BsiSI | CCGG | 3 cut(s) | 89, 780, 815 |
| BslFI | GGGAC | 1 cut(s) | 257 |
| BsmAI | GTCTC | 1 cut(s) | 409 |
| BsmFI | GGGAC | 1 cut(s) | 257 |
| BsnI | GGCC | 2 cut(s) | 88, 526 |
| BsoBI | CYCGRG | 1 cut(s) | 485 |
| Bsp1286I | GDGCHC | 3 cut(s) | 594, 903, 984 |
| Bsp1407I | TGTACA | 1 cut(s) | 885 |
| Bsp143I | GATC | 4 cut(s) | 74, 121, 599, 988 |
| BspACI | CCGC | 2 cut(s) | 179, 353 |
| BspANI | GGCC | 2 cut(s) | 88, 526 |
| BspCNI | CTCAG | 5 cut(s) | 196, 424, 439, 508, 588 |
| BspLI | GGNNCC | 3 cut(s) | 93, 135, 813 |
| BspOI | GCTAGC | 1 cut(s) | 747 |
| BspPI | GGATC | 1 cut(s) | 116 |
| BspT107I | GGYRCC | 2 cut(s) | 91, 811 |
| BsrFI | RCCGGY | 2 cut(s) | 88, 814 |
| BsrGI | TGTACA | 1 cut(s) | 885 |
| BsrI | ACTGG | 1 cut(s) | 114 |
| BssAI | RCCGGY | 2 cut(s) | 88, 814 |
| BssMI | GATC | 4 cut(s) | 74, 121, 599, 988 |
| Bst2UI | CCWGG | 1 cut(s) | 272 |
| Bst4CI | ACNGT | 1 cut(s) | 773 |
| Bst6I | CTCTTC | 2 cut(s) | 87, 375 |
| BstAUI | TGTACA | 1 cut(s) | 885 |
| BstC8I | GCNNGC | 3 cut(s) | 277, 660, 745 |
| BstDEI | CTNAG | 5 cut(s) | 204, 411, 447, 516, 596 |
| BstF5I | GGATG | 4 cut(s) | 352, 627, 637, 888 |
| BstHHI | GCGC | 1 cut(s) | 958 |
| BstKTI | GATC | 4 cut(s) | 77, 124, 602, 991 |
| BstMAI | GTCTC | 1 cut(s) | 409 |
| BstMBI | GATC | 4 cut(s) | 74, 121, 599, 988 |
| BstMCI | CGRYCG | 1 cut(s) | 89 |
| BstNI | CCWGG | 1 cut(s) | 272 |
| BstNSI | RCATGY | 2 cut(s) | 169, 581 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 771 |
| BstSCI | CCNGG | 1 cut(s) | 270 |
| BstV1I | GCAGC | 1 cut(s) | 337 |
| BstV2I | GAAGAC | 1 cut(s) | 168 |
| BstX2I | RGATCY | 1 cut(s) | 599 |
| BstYI | RGATCY | 1 cut(s) | 599 |
| BstZI | CGGCCG | 1 cut(s) | 86 |
| Bsu36I | CCTNAGG | 1 cut(s) | 516 |
| BsuRI | GGCC | 2 cut(s) | 88, 526 |
| BtsCI | GGATG | 4 cut(s) | 352, 627, 637, 888 |
| Cac8I | GCNNGC | 3 cut(s) | 277, 660, 745 |
| CfoI | GCGC | 1 cut(s) | 958 |
| Cfr10I | RCCGGY | 2 cut(s) | 88, 814 |
| Csp6I | GTAC | 5 cut(s) | 92, 713, 812, 886, 998 |
| CspAI | ACCGGT | 1 cut(s) | 814 |
| CviAII | CATG | 6 cut(s) | 166, 311, 578, 693, 797, 962 |
| CviQI | GTAC | 5 cut(s) | 92, 713, 812, 886, 998 |
| DdeI | CTNAG | 5 cut(s) | 204, 411, 447, 516, 596 |
| DpnI | GATC | 4 cut(s) | 76, 123, 601, 990 |
| DpnII | GATC | 4 cut(s) | 74, 121, 599, 988 |
| DraI | TTTAAA | 1 cut(s) | 9 |
| EaeI | YGGCCR | 2 cut(s) | 86, 524 |
| EagI | CGGCCG | 1 cut(s) | 86 |
| Eam1104I | CTCTTC | 2 cut(s) | 87, 375 |
| EarI | CTCTTC | 2 cut(s) | 87, 375 |
| Ecl136II | GAGCTC | 1 cut(s) | 592 |
| EclXI | CGGCCG | 1 cut(s) | 86 |
| Eco24I | GRGCYC | 2 cut(s) | 594, 984 |
| Eco52I | CGGCCG | 1 cut(s) | 86 |
| Eco53kI | GAGCTC | 1 cut(s) | 592 |
| Eco81I | CCTNAGG | 1 cut(s) | 516 |
| Eco88I | CYCGRG | 1 cut(s) | 485 |
| EcoICRI | GAGCTC | 1 cut(s) | 592 |
| EcoRI | GAATTC | 1 cut(s) | 477 |
| EcoRII | CCWGG | 1 cut(s) | 270 |
| EcoT22I | ATGCAT | 1 cut(s) | 946 |
| EcoT38I | GRGCYC | 2 cut(s) | 594, 984 |
| FaeI | CATG | 6 cut(s) | 169, 314, 581, 696, 800, 965 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 922, 954 |
| FaqI | GGGAC | 1 cut(s) | 257 |
| FatI | CATG | 6 cut(s) | 165, 310, 577, 692, 796, 961 |
| FbaI | TGATCA | 1 cut(s) | 988 |
| Fnu4HI | GCNGC | 1 cut(s) | 351 |
| FokI | GGATG | 4 cut(s) | 359, 634, 644, 895 |
| FriOI | GRGCYC | 2 cut(s) | 594, 984 |
| Fsp4HI | GCNGC | 1 cut(s) | 351 |
| FspBI | CTAG | 2 cut(s) | 716, 744 |
| GlaI | GCGC | 1 cut(s) | 957 |
| GluI | GCNGC | 1 cut(s) | 351 |
| GsuI | CTGGAG | 1 cut(s) | 993 |
| HaeIII | GGCC | 2 cut(s) | 88, 526 |
| HapII | CCGG | 3 cut(s) | 89, 780, 815 |
| HhaI | GCGC | 1 cut(s) | 958 |
| Hin1II | CATG | 6 cut(s) | 169, 314, 581, 696, 800, 965 |
| Hin6I | GCGC | 1 cut(s) | 956 |
| HinP1I | GCGC | 1 cut(s) | 956 |
| HinfI | GANTC | 5 cut(s) | 44, 208, 291, 906, 1013 |
| HpaII | CCGG | 3 cut(s) | 89, 780, 815 |
| HphI | GGTGA | 1 cut(s) | 829 |
| Hpy188I | TCNGA | 6 cut(s) | 205, 414, 448, 472, 736, 1023 |
| Hpy188III | TCNNGA | 7 cut(s) | 104, 595, 641, 653, 910, 1010, 1069 |
| Hpy99I | CGWCG | 1 cut(s) | 773 |
| HpyAV | CCTTC | 5 cut(s) | 166, 622, 699, 794, 1055 |
| HpyCH4III | ACNGT | 1 cut(s) | 773 |
| HpyCH4V | TGCA | 7 cut(s) | 31, 279, 334, 584, 763, 944, 971 |
| HpyF3I | CTNAG | 5 cut(s) | 204, 411, 447, 516, 596 |
| Hsp92II | CATG | 6 cut(s) | 169, 314, 581, 696, 800, 965 |
| HspAI | GCGC | 1 cut(s) | 956 |
| KpnI | GGTACC | 2 cut(s) | 95, 815 |
| Ksp22I | TGATCA | 1 cut(s) | 988 |
| Kzo9I | GATC | 4 cut(s) | 74, 121, 599, 988 |
| LmnI | GCTCC | 3 cut(s) | 253, 589, 597 |
| Lsp1109I | GCAGC | 1 cut(s) | 337 |
| LweI | GCATC | 5 cut(s) | 337, 348, 847, 953, 958 |
| MaeI | CTAG | 2 cut(s) | 716, 744 |
| MaeIII | GTNAC | 1 cut(s) | 910 |
| MalI | GATC | 4 cut(s) | 76, 123, 601, 990 |
| MboI | GATC | 4 cut(s) | 74, 121, 599, 988 |
| MflI | RGATCY | 1 cut(s) | 599 |
| MhlI | GDGCHC | 3 cut(s) | 594, 903, 984 |
| MlsI | TGGCCA | 1 cut(s) | 526 |
| MluCI | AATT | 9 cut(s) | 388, 441, 477, 665, 825, 975, 1079, 1098, 1103 |
| MluNI | TGGCCA | 1 cut(s) | 526 |
| MlyI | GAGTC | 2 cut(s) | 915, 1022 |
| MmeI | TCCRAC | 2 cut(s) | 759, 832 |
| MnlI | CCTC | 6 cut(s) | 28, 244, 442, 511, 580, 738 |
| Mox20I | TGGCCA | 1 cut(s) | 526 |
| Mph1103I | ATGCAT | 1 cut(s) | 946 |
| MscI | TGGCCA | 1 cut(s) | 526 |
| MseI | TTAA | 4 cut(s) | 8, 432, 792, 1056 |
| MslI | CAYNNNNRTG | 3 cut(s) | 17, 758, 966 |
| Msp20I | TGGCCA | 1 cut(s) | 526 |
| MspI | CCGG | 3 cut(s) | 89, 780, 815 |
| MspR9I | CCNGG | 1 cut(s) | 272 |
| MssI | GTTTAAAC | 1 cut(s) | 9 |
| MvaI | CCWGG | 1 cut(s) | 272 |
| NdeII | GATC | 4 cut(s) | 74, 121, 599, 988 |
| NheI | GCTAGC | 1 cut(s) | 743 |
| NlaIII | CATG | 6 cut(s) | 169, 314, 581, 696, 800, 965 |
| NlaIV | GGNNCC | 3 cut(s) | 93, 135, 813 |
| NmuCI | GTSAC | 1 cut(s) | 910 |
| NsiI | ATGCAT | 1 cut(s) | 946 |
| NspI | RCATGY | 2 cut(s) | 169, 581 |
| OliI | CACNNNNGTG | 1 cut(s) | 17 |
| PciI | ACATGT | 1 cut(s) | 577 |
| PfeI | GAWTC | 3 cut(s) | 44, 208, 291 |
| PinAI | ACCGGT | 1 cut(s) | 814 |
| PkrI | GCNGC | 1 cut(s) | 352 |
| PleI | GAGTC | 2 cut(s) | 914, 1021 |
| PmeI | GTTTAAAC | 1 cut(s) | 9 |
| PpsI | GAGTC | 2 cut(s) | 914, 1021 |
| PscI | ACATGT | 1 cut(s) | 577 |
| PshAI | GACNNNNGTC | 1 cut(s) | 771 |
| Psp124BI | GAGCTC | 1 cut(s) | 594 |
| Psp6I | CCWGG | 1 cut(s) | 270 |
| PspGI | CCWGG | 1 cut(s) | 270 |
| PspN4I | GGNNCC | 3 cut(s) | 93, 135, 813 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 84, 116 |
| PsuI | RGATCY | 1 cut(s) | 599 |
| RsaI | GTAC | 5 cut(s) | 93, 714, 813, 887, 999 |
| RsaNI | GTAC | 5 cut(s) | 92, 713, 812, 886, 998 |
| RseI | CAYNNNNRTG | 3 cut(s) | 17, 758, 966 |
| SacI | GAGCTC | 1 cut(s) | 594 |
| SaqAI | TTAA | 4 cut(s) | 8, 432, 792, 1056 |
| SatI | GCNGC | 1 cut(s) | 351 |
| Sau3AI | GATC | 4 cut(s) | 74, 121, 599, 988 |
| ScaI | AGTACT | 1 cut(s) | 714 |
| SchI | GAGTC | 2 cut(s) | 915, 1022 |
| ScrFI | CCNGG | 1 cut(s) | 272 |
| SduI | GDGCHC | 3 cut(s) | 594, 903, 984 |
| SfaNI | GCATC | 5 cut(s) | 337, 348, 847, 953, 958 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 17, 758, 966 |
| SmlI | CTYRAG | 1 cut(s) | 539 |
| SmoI | CTYRAG | 1 cut(s) | 539 |
| Sse9I | AATT | 9 cut(s) | 388, 441, 477, 665, 825, 975, 1079, 1098, 1103 |
| SsiI | CCGC | 2 cut(s) | 179, 353 |
| SspMI | CTAG | 2 cut(s) | 716, 744 |
| SstI | GAGCTC | 1 cut(s) | 594 |
| StyD4I | CCNGG | 1 cut(s) | 270 |
| TaaI | ACNGT | 1 cut(s) | 773 |
| TaqI | TCGA | 3 cut(s) | 267, 502, 1070 |
| TasI | AATT | 9 cut(s) | 388, 441, 477, 665, 825, 975, 1079, 1098, 1103 |
| TatI | WGTACW | 2 cut(s) | 712, 885 |
| TfiI | GAWTC | 3 cut(s) | 44, 208, 291 |
| Tru1I | TTAA | 4 cut(s) | 8, 432, 792, 1056 |
| Tru9I | TTAA | 4 cut(s) | 8, 432, 792, 1056 |
| TseFI | GTSAC | 1 cut(s) | 910 |
| TseI | GCWGC | 1 cut(s) | 350 |
| Tsp45I | GTSAC | 1 cut(s) | 910 |
| TspDTI | ATGAA | 4 cut(s) | 681, 813, 933, 978 |
| XapI | RAATTY | 4 cut(s) | 388, 477, 1079, 1103 |
| XceI | RCATGY | 2 cut(s) | 169, 581 |
| XspI | CTAG | 2 cut(s) | 716, 744 |
| ZrmI | AGTACT | 1 cut(s) | 714 |
| Zsp2I | ATGCAT | 1 cut(s) | 946 |