Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 604 |
| AccB1I | GGYRCC | 3 cut(s) | 82, 536, 604 |
| AciI | CCGC | 1 cut(s) | 15 |
| AclI | AACGTT | 1 cut(s) | 740 |
| AclWI | GGATC | 2 cut(s) | 13, 244 |
| AcoI | YGGCCR | 2 cut(s) | 317, 321 |
| AfaI | GTAC | 4 cut(s) | 507, 606, 792, 821 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 79, 467 |
| AgsI | TTSAA | 6 cut(s) | 53, 334, 692, 738, 773, 875 |
| AjiI | CACGTC | 1 cut(s) | 755 |
| AjnI | CCWGG | 2 cut(s) | 219, 252 |
| AluBI | AGCT | 6 cut(s) | 205, 214, 307, 385, 451, 907 |
| AluI | AGCT | 6 cut(s) | 205, 214, 307, 385, 451, 907 |
| Alw21I | GWGCWC | 1 cut(s) | 387 |
| AlwI | GGATC | 2 cut(s) | 13, 244 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 253 |
| Ama87I | CYCGRG | 2 cut(s) | 308, 794 |
| AoxI | GGCC | 3 cut(s) | 317, 321, 517 |
| Asp718I | GGTACC | 1 cut(s) | 604 |
| AspLEI | GCGC | 1 cut(s) | 886 |
| AspS9I | GGNCC | 3 cut(s) | 217, 261, 518 |
| AsuHPI | GGTGA | 1 cut(s) | 73 |
| AvaI | CYCGRG | 2 cut(s) | 308, 794 |
| AvaII | GGWCC | 2 cut(s) | 217, 261 |
| AxyI | CCTNAGG | 1 cut(s) | 389 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 782, 815 |
| BanI | GGYRCC | 3 cut(s) | 82, 536, 604 |
| BanII | GRGCYC | 1 cut(s) | 387 |
| Bbv12I | GWGCWC | 1 cut(s) | 387 |
| BccI | CCATC | 3 cut(s) | 170, 409, 649 |
| BciT130I | CCWGG | 2 cut(s) | 221, 254 |
| BclI | TGATCA | 1 cut(s) | 781 |
| BfaI | CTAG | 2 cut(s) | 357, 509 |
| BfmI | CTRYAG | 1 cut(s) | 298 |
| BlpI | GCTNAGC | 1 cut(s) | 903 |
| BmcAI | AGTACT | 1 cut(s) | 507 |
| Bme1390I | CCNGG | 2 cut(s) | 221, 254 |
| Bme18I | GGWCC | 2 cut(s) | 217, 261 |
| BmeT110I | CYCGRG | 2 cut(s) | 308, 794 |
| BmgBI | CACGTC | 1 cut(s) | 755 |
| BmgT120I | GGNCC | 3 cut(s) | 217, 261, 518 |
| BmiI | GGNNCC | 5 cut(s) | 84, 159, 472, 538, 606 |
| BmrFI | CCNGG | 2 cut(s) | 221, 254 |
| BmsI | GCATC | 2 cut(s) | 115, 415 |
| BoxI | GACNNNNGTC | 1 cut(s) | 135 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 369, 401 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 903 |
| Bsa29I | ATCGAT | 1 cut(s) | 168 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 79, 467 |
| Bse118I | RCCGGY | 1 cut(s) | 319 |
| Bse21I | CCTNAGG | 1 cut(s) | 389 |
| Bse3DI | GCAATG | 1 cut(s) | 114 |
| BseBI | CCWGG | 2 cut(s) | 221, 254 |
| BseCI | ATCGAT | 1 cut(s) | 168 |
| BseGI | GGATG | 4 cut(s) | 286, 420, 430, 571 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 79, 467 |
| BseMI | GCAATG | 1 cut(s) | 114 |
| BseMII | CTCAG | 3 cut(s) | 144, 497, 540 |
| BseRI | GAGGAG | 3 cut(s) | 79, 215, 395 |
| BshFI | GGCC | 3 cut(s) | 319, 323, 519 |
| BshNI | GGYRCC | 3 cut(s) | 82, 536, 604 |
| BshVI | ATCGAT | 1 cut(s) | 168 |
| BsiHKAI | GWGCWC | 1 cut(s) | 387 |
| BsiHKCI | CYCGRG | 2 cut(s) | 308, 794 |
| BsiSI | CCGG | 2 cut(s) | 320, 803 |
| BslFI | GGGAC | 2 cut(s) | 588, 793 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 79, 467 |
| BsmFI | GGGAC | 2 cut(s) | 588, 793 |
| BsnI | GGCC | 3 cut(s) | 319, 323, 519 |
| BsoBI | CYCGRG | 2 cut(s) | 308, 794 |
| Bsp1286I | GDGCHC | 1 cut(s) | 387 |
| Bsp1407I | TGTACA | 1 cut(s) | 819 |
| Bsp143I | GATC | 4 cut(s) | 5, 249, 609, 781 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 903 |
| BspACI | CCGC | 1 cut(s) | 15 |
| BspANI | GGCC | 3 cut(s) | 319, 323, 519 |
| BspCNI | CTCAG | 3 cut(s) | 145, 496, 539 |
| BspDI | ATCGAT | 1 cut(s) | 168 |
| BspHI | TCATGA | 1 cut(s) | 757 |
| BspLI | GGNNCC | 5 cut(s) | 84, 159, 472, 538, 606 |
| BspPI | GGATC | 2 cut(s) | 13, 244 |
| BspT107I | GGYRCC | 3 cut(s) | 82, 536, 604 |
| BsrDI | GCAATG | 1 cut(s) | 114 |
| BsrFI | RCCGGY | 1 cut(s) | 319 |
| BsrGI | TGTACA | 1 cut(s) | 819 |
| BssAI | RCCGGY | 1 cut(s) | 319 |
| BssMI | GATC | 4 cut(s) | 5, 249, 609, 781 |
| Bst2UI | CCWGG | 2 cut(s) | 221, 254 |
| Bst6I | CTCTTC | 1 cut(s) | 555 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 125 |
| BstAUI | TGTACA | 1 cut(s) | 819 |
| BstC8I | GCNNGC | 2 cut(s) | 226, 321 |
| BstDEI | CTNAG | 7 cut(s) | 153, 389, 483, 526, 848, 858, 903 |
| BstF5I | GGATG | 4 cut(s) | 286, 420, 430, 571 |
| BstHHI | GCGC | 1 cut(s) | 886 |
| BstKTI | GATC | 4 cut(s) | 8, 252, 612, 784 |
| BstMBI | GATC | 4 cut(s) | 5, 249, 609, 781 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 23, 125, 211, 347, 543, 859 |
| BstNI | CCWGG | 2 cut(s) | 221, 254 |
| BstNSI | RCATGY | 2 cut(s) | 496, 890 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 135 |
| BstSCI | CCNGG | 2 cut(s) | 219, 252 |
| BstSFI | CTRYAG | 1 cut(s) | 298 |
| BstX2I | RGATCY | 1 cut(s) | 249 |
| BstYI | RGATCY | 1 cut(s) | 249 |
| Bsu15I | ATCGAT | 1 cut(s) | 168 |
| Bsu36I | CCTNAGG | 1 cut(s) | 389 |
| BsuRI | GGCC | 3 cut(s) | 319, 323, 519 |
| BsuTUI | ATCGAT | 1 cut(s) | 168 |
| BtrI | CACGTC | 1 cut(s) | 755 |
| BtsCI | GGATG | 4 cut(s) | 286, 420, 430, 571 |
| BtsIMutI | CAGTG | 1 cut(s) | 491 |
| Cac8I | GCNNGC | 2 cut(s) | 226, 321 |
| CaiI | CAGNNNCTG | 1 cut(s) | 253 |
| CciI | TCATGA | 1 cut(s) | 757 |
| CfoI | GCGC | 1 cut(s) | 886 |
| Cfr10I | RCCGGY | 1 cut(s) | 319 |
| Cfr13I | GGNCC | 3 cut(s) | 217, 261, 518 |
| ClaI | ATCGAT | 1 cut(s) | 168 |
| Csp6I | GTAC | 4 cut(s) | 506, 605, 791, 820 |
| CviAII | CATG | 5 cut(s) | 30, 463, 493, 758, 887 |
| CviQI | GTAC | 4 cut(s) | 506, 605, 791, 820 |
| DdeI | CTNAG | 7 cut(s) | 153, 389, 483, 526, 848, 858, 903 |
| DpnI | GATC | 4 cut(s) | 7, 251, 611, 783 |
| DpnII | GATC | 4 cut(s) | 5, 249, 609, 781 |
| EaeI | YGGCCR | 2 cut(s) | 317, 321 |
| Eam1104I | CTCTTC | 1 cut(s) | 555 |
| EarI | CTCTTC | 1 cut(s) | 555 |
| EciI | GGCGGA | 1 cut(s) | 4 |
| Ecl136II | GAGCTC | 1 cut(s) | 385 |
| Eco24I | GRGCYC | 1 cut(s) | 387 |
| Eco32I | GATATC | 1 cut(s) | 729 |
| Eco47I | GGWCC | 2 cut(s) | 217, 261 |
| Eco53kI | GAGCTC | 1 cut(s) | 385 |
| Eco81I | CCTNAGG | 1 cut(s) | 389 |
| Eco88I | CYCGRG | 2 cut(s) | 308, 794 |
| EcoICRI | GAGCTC | 1 cut(s) | 385 |
| EcoRII | CCWGG | 2 cut(s) | 219, 252 |
| EcoRV | GATATC | 1 cut(s) | 729 |
| EcoT38I | GRGCYC | 1 cut(s) | 387 |
| FaeI | CATG | 5 cut(s) | 33, 466, 496, 761, 890 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 189, 221 |
| FaqI | GGGAC | 2 cut(s) | 588, 793 |
| FatI | CATG | 5 cut(s) | 29, 462, 492, 757, 886 |
| FauNDI | CATATG | 1 cut(s) | 864 |
| FbaI | TGATCA | 1 cut(s) | 781 |
| FokI | GGATG | 4 cut(s) | 273, 427, 437, 578 |
| FriOI | GRGCYC | 1 cut(s) | 387 |
| FseI | GGCCGGCC | 1 cut(s) | 323 |
| FspBI | CTAG | 2 cut(s) | 357, 509 |
| GlaI | GCGC | 1 cut(s) | 885 |
| HaeIII | GGCC | 3 cut(s) | 319, 323, 519 |
| HapII | CCGG | 2 cut(s) | 320, 803 |
| HhaI | GCGC | 1 cut(s) | 886 |
| Hin1II | CATG | 5 cut(s) | 33, 466, 496, 761, 890 |
| Hin6I | GCGC | 1 cut(s) | 884 |
| HinP1I | GCGC | 1 cut(s) | 884 |
| HindIII | AAGCTT | 1 cut(s) | 905 |
| HinfI | GANTC | 3 cut(s) | 240, 797, 806 |
| HpaII | CCGG | 2 cut(s) | 320, 803 |
| HphI | GGTGA | 1 cut(s) | 73 |
| Hpy188I | TCNGA | 5 cut(s) | 154, 249, 265, 529, 816 |
| Hpy188III | TCNNGA | 6 cut(s) | 53, 100, 446, 468, 623, 758 |
| HpyAV | CCTTC | 5 cut(s) | 415, 587, 821, 869, 874 |
| HpyCH4IV | ACGT | 2 cut(s) | 740, 754 |
| HpyCH4V | TGCA | 5 cut(s) | 119, 128, 228, 457, 556 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 23, 125, 211, 347, 543, 859 |
| HpyF3I | CTNAG | 7 cut(s) | 153, 389, 483, 526, 848, 858, 903 |
| HpySE526I | ACGT | 2 cut(s) | 740, 754 |
| Hsp92II | CATG | 5 cut(s) | 33, 466, 496, 761, 890 |
| HspAI | GCGC | 1 cut(s) | 884 |
| KpnI | GGTACC | 1 cut(s) | 608 |
| KroI | GCCGGC | 1 cut(s) | 319 |
| KroNI | GCCGGC | 1 cut(s) | 321 |
| Ksp22I | TGATCA | 1 cut(s) | 781 |
| Kzo9I | GATC | 4 cut(s) | 5, 249, 609, 781 |
| LmnI | GCTCC | 4 cut(s) | 202, 382, 390, 470 |
| LweI | GCATC | 2 cut(s) | 115, 415 |
| MaeI | CTAG | 2 cut(s) | 357, 509 |
| MaeII | ACGT | 2 cut(s) | 740, 754 |
| MaeIII | GTNAC | 3 cut(s) | 68, 568, 703 |
| MalI | GATC | 4 cut(s) | 7, 251, 611, 783 |
| MboI | GATC | 4 cut(s) | 5, 249, 609, 781 |
| MboII | GAAGA | 1 cut(s) | 572 |
| MflI | RGATCY | 1 cut(s) | 249 |
| MhlI | GDGCHC | 1 cut(s) | 387 |
| MluCI | AATT | 3 cut(s) | 618, 768, 910 |
| MlyI | GAGTC | 2 cut(s) | 806, 815 |
| MmeI | TCCRAC | 3 cut(s) | 288, 355, 625 |
| MroNI | GCCGGC | 1 cut(s) | 319 |
| MseI | TTAA | 3 cut(s) | 366, 894, 909 |
| MslI | CAYNNNNRTG | 1 cut(s) | 551 |
| MspI | CCGG | 2 cut(s) | 320, 803 |
| MspR9I | CCNGG | 2 cut(s) | 221, 254 |
| MvaI | CCWGG | 2 cut(s) | 221, 254 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 23, 125, 211, 347, 543, 859 |
| NaeI | GCCGGC | 1 cut(s) | 321 |
| NdeI | CATATG | 1 cut(s) | 864 |
| NdeII | GATC | 4 cut(s) | 5, 249, 609, 781 |
| NgoMIV | GCCGGC | 1 cut(s) | 319 |
| NlaIII | CATG | 5 cut(s) | 33, 466, 496, 761, 890 |
| NlaIV | GGNNCC | 5 cut(s) | 84, 159, 472, 538, 606 |
| NmuCI | GTSAC | 1 cut(s) | 68 |
| NspI | RCATGY | 2 cut(s) | 496, 890 |
| PaeR7I | CTCGAG | 2 cut(s) | 308, 794 |
| PagI | TCATGA | 1 cut(s) | 757 |
| PdiI | GCCGGC | 1 cut(s) | 321 |
| PfeI | GAWTC | 1 cut(s) | 240 |
| PfoI | TCCNGGA | 1 cut(s) | 252 |
| PleI | GAGTC | 2 cut(s) | 805, 814 |
| PpsI | GAGTC | 2 cut(s) | 805, 814 |
| PshAI | GACNNNNGTC | 1 cut(s) | 135 |
| Psp124BI | GAGCTC | 1 cut(s) | 387 |
| Psp1406I | AACGTT | 1 cut(s) | 740 |
| Psp6I | CCWGG | 2 cut(s) | 219, 252 |
| PspGI | CCWGG | 2 cut(s) | 219, 252 |
| PspN4I | GGNNCC | 5 cut(s) | 84, 159, 472, 538, 606 |
| PspPI | GGNCC | 3 cut(s) | 217, 261, 518 |
| PspXI | VCTCGAGB | 2 cut(s) | 308, 794 |
| PstNI | CAGNNNCTG | 1 cut(s) | 253 |
| PsuI | RGATCY | 1 cut(s) | 249 |
| RigI | GGCCGGCC | 1 cut(s) | 323 |
| RsaI | GTAC | 4 cut(s) | 507, 606, 792, 821 |
| RsaNI | GTAC | 4 cut(s) | 506, 605, 791, 820 |
| RseI | CAYNNNNRTG | 1 cut(s) | 551 |
| SacI | GAGCTC | 1 cut(s) | 387 |
| SaqAI | TTAA | 3 cut(s) | 366, 894, 909 |
| Sau3AI | GATC | 4 cut(s) | 5, 249, 609, 781 |
| Sau96I | GGNCC | 3 cut(s) | 217, 261, 518 |
| ScaI | AGTACT | 1 cut(s) | 507 |
| SchI | GAGTC | 2 cut(s) | 806, 815 |
| ScrFI | CCNGG | 2 cut(s) | 221, 254 |
| SduI | GDGCHC | 1 cut(s) | 387 |
| SfaNI | GCATC | 2 cut(s) | 115, 415 |
| SfcI | CTRYAG | 1 cut(s) | 298 |
| Sfr274I | CTCGAG | 2 cut(s) | 308, 794 |
| SinI | GGWCC | 2 cut(s) | 217, 261 |
| SlaI | CTCGAG | 2 cut(s) | 308, 794 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 551 |
| SmlI | CTYRAG | 2 cut(s) | 308, 794 |
| SmoI | CTYRAG | 2 cut(s) | 308, 794 |
| Sse9I | AATT | 3 cut(s) | 618, 768, 910 |
| SsiI | CCGC | 1 cut(s) | 15 |
| SspMI | CTAG | 2 cut(s) | 357, 509 |
| SstI | GAGCTC | 1 cut(s) | 387 |
| StyD4I | CCNGG | 2 cut(s) | 219, 252 |
| TaiI | ACGT | 2 cut(s) | 743, 757 |
| TaqI | TCGA | 3 cut(s) | 168, 309, 795 |
| TasI | AATT | 3 cut(s) | 618, 768, 910 |
| TatI | WGTACW | 2 cut(s) | 505, 819 |
| TfiI | GAWTC | 1 cut(s) | 240 |
| Tru1I | TTAA | 3 cut(s) | 366, 894, 909 |
| Tru9I | TTAA | 3 cut(s) | 366, 894, 909 |
| TscAI | CASTG | 1 cut(s) | 491 |
| TseFI | GTSAC | 1 cut(s) | 68 |
| Tsp45I | GTSAC | 1 cut(s) | 68 |
| TspDTI | ATGAA | 2 cut(s) | 120, 275 |
| TspRI | CASTG | 1 cut(s) | 491 |
| VpaK11BI | GGWCC | 2 cut(s) | 217, 261 |
| XceI | RCATGY | 2 cut(s) | 496, 890 |
| XhoI | CTCGAG | 2 cut(s) | 308, 794 |
| XspI | CTAG | 2 cut(s) | 357, 509 |
| ZrmI | AGTACT | 1 cut(s) | 507 |