Rroxscaffold_6G00414750

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000451
Physical Location & Seq
Reverse (-)
37022324 .. 37024666
2343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00414750.1

Sequence Viewer

Length: 1074 bp
ATGAGTTTAAACACAGATGTGGCGTTTGTGCAAGAGCATAAGGATTCTGGGAGTTTTTCCCAGAAACCACATAGATCATCTCTTCGGCCGGTACCAAATGTTCAAGAACTGGTAAGGAATGATCCTTTACAGGTTCCCAAAAGATATATTAGGAACGAAGAAGACATGCCGAAGGAAGCGGATCTTTGTCATCATCTTTCTTCTGAGATTCCTATCATTGATGTTTCTTTGCTCTCCAAAGGGGACAACGAGGAGCTTCGCAAACTCGACCTGGCTTGCAAAGAATGGGGATTCTTTGTGATGATAAATCATGGAGTGGAAACAGAAGTAATGCAGGGCATGAAGGATGCTGCGGACAAGTTCTTTGAACTTCCAATAGAAGAGAAGAATTTTATTGCTGTGCCATCAGCTGAGAGACAAGGCTATGGTTTTAACCAAGCAATTTCTGAGGGGCAGATTTTGGATTGGTCTGACAGAATTCTTCTCGGGCTTTACCCATATCGACATAGAAAGCCTGAGGTTTGGCCACCGACACCACTCAAGGAAGCTATTGAGATATATGGTAGTGAAGTTAGACATGTTGCAGAGGAGCTCCTGAGATCTCTATCTTTGATTATGGGGATGGAGAAGGATGTTCTTCTTGAACTACATCAAGAGCTGCCTCAATTTCTGTCTGTGGCCTATTATCCTTCATGCTCTATGCCTGATAAAGTACTAGGTCTAACCCCTCACTCCGACAAAGGTAGCATAACCATACTTATGCAAGACGACAGTCTTACCGGACTACAACTTAAACATGAAGGAAAATGGGTACCGATATGGAGTAATGGGATGTACAAGAGCATTGAGCACAGAGTTGTGACAAATGAAAGCAAAGCAAGGTTATCTTATGCATCATTTTTTGTGCCACATGAAGATGCAGAAATTGAGCCCTTTCATCAAATGGTACTCAACTCTCCAGAGTCCATTCAGATATACAAGAAAGTCAAATATGGAGAATATATTAAGGAAGGTGTCTCGAAGAAGAAATTTTATGAGAAAGAACAAATTGAATTTGCCAAGATTGGAAGTTAG

Protein Analysis

357

Amino Acids

40.99

Weight (kDa)

5.53

Isoelectric Point (pI)

51.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 70 - 177 1.5e-24 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 223 - 302 1.3e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000451)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13280 FvH4_6g13281 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13300 FvH4_6g13322
malus_domestica MD04G1132000.v1.1 MD04G1132100.v1.1 MD04G1132400.v1.1 MD12G1145100.v1.1 MD12G1145200.v1.1
prunus_persica Prupe.6G258600_v2.0.a1 Prupe.6G258700_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258900_v2.0.a1
pyrus_communis pycom04g12010 pycom04g12020
rosa_chinensis RchiOBHm_Chr3g0466131 RchiOBHm_Chr3g0466151 RchiOBHm_Chr3g0466161 RchiOBHm_Chr3g0466171 RchiOBHm_Chr3g0466181 RchiOBHm_Chr3g0466201 RchiOBHm_Chr3g0466211 RchiOBHm_Chr6g0251841
rosa_laevigata RLG00000015425 RLG00000024596 RLG00000024599 RLG00000024600 RLG00000024601 RLG00000024602
rosa_multiflora Rmu_co8112718.1_g000001 Rmu_co8178858.1_g000001 Rmu_co8388385.1_g000001 Rmu_sc0000350.1_g000002 Rmu_sc0000350.1_g000003 Rmu_sc0000350.1_g000004 Rmu_sc0000350.1_g000006 Rmu_sc0000350.1_g000011 Rmu_sc0002249.1_g000004 Rmu_sc0007597.1_g000012 Rmu_sc0012177.1_g000002 Rmu_sc0012177.1_g000006 Rmu_sc0012177.1_g000007 Rmu_sc0022084.1_g000003 Rmu_ssc0000303.1_g000004
rosa_roxburghii Rroxscaffold_6G00414720 Rroxscaffold_6G00414730 Rroxscaffold_6G00414740 Rroxscaffold_6G00414750 Rroxscaffold_6G00414760 Rroxscaffold_6G00414770 Rroxscaffold_6G00414780 Rroxscaffold_6G00414790
rosa_rugosa Rorug03G0079600 Rorug03G0079600 Rorug03G0079800 Rorug03G0080100 Rorug03G0080200 Rorug03G0080300 Rorug03G0080400 Rorug05G0504900
rosa_samantha Rh3BG147200 Rh3BG147300 Rh3BG147400 Rh3BG147500 Rh3BG147600 Rh3CG147900 Rh3CG148000 Rh3CG148100 Rh3CG148200 Rh3CG148300 Rh3CG148500 Rh3CG148600 Rh3DG147800 Rh3DG147900 Rh3DG148100 Rh3DG148300 Rh4BG237100 Rh6AG015600 Rh6AG015700 Rh6BG013700 Rh6CG069500
rosa_wichuraiana Rw3G011870 Rw3G011880 Rw3G011890 Rw3G011900 Rw3G011910 Rw3G011930 Rw6G001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 91, 811
AccB1I GGYRCC 2 cut(s) 91, 811
AciI CCGC 2 cut(s) 179, 353
AclWI GGATC 2 cut(s) 116, 189
AcoI YGGCCR 2 cut(s) 86, 524
AcsI RAATTY 4 cut(s) 388, 477, 1028, 1052
AfaI GTAC 5 cut(s) 93, 714, 813, 836, 948
AflIII ACRYGT 1 cut(s) 577
AgsI TTSAA 4 cut(s) 104, 368, 644, 1052
AjnI CCWGG 1 cut(s) 270
AleI CACNNNNGTG 1 cut(s) 17
AluBI AGCT 5 cut(s) 256, 410, 548, 592, 658
AluI AGCT 5 cut(s) 256, 410, 548, 592, 658
Alw21I GWGCWC 2 cut(s) 594, 852
Alw26I GTCTC 2 cut(s) 409, 1021
AlwI GGATC 2 cut(s) 116, 189
Ama87I CYCGRG 1 cut(s) 485
AoxI GGCC 3 cut(s) 86, 524, 678
ApeKI GCWGC 2 cut(s) 350, 658
ApoI RAATTY 4 cut(s) 388, 477, 1028, 1052
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
Asp718I GGTACC 2 cut(s) 91, 811
AvaI CYCGRG 1 cut(s) 485
AxyI CCTNAGG 1 cut(s) 516
BalI TGGCCA 1 cut(s) 526
BanI GGYRCC 2 cut(s) 91, 811
BanII GRGCYC 2 cut(s) 594, 933
BbsI GAAGAC 1 cut(s) 168
Bbv12I GWGCWC 2 cut(s) 594, 852
BbvI GCAGC 2 cut(s) 337, 645
BccI CCATC 2 cut(s) 412, 616
BciT130I CCWGG 1 cut(s) 272
BcoDI GTCTC 2 cut(s) 409, 1021
BfaI CTAG 1 cut(s) 716
BglII AGATCT 1 cut(s) 599
BisI GCNGC 2 cut(s) 351, 659
BlsI GCNGC 2 cut(s) 352, 660
BmcAI AGTACT 1 cut(s) 714
Bme1390I CCNGG 1 cut(s) 272
BmeT110I CYCGRG 1 cut(s) 485
BmiI GGNNCC 3 cut(s) 93, 135, 813
BmrFI CCNGG 1 cut(s) 272
BmsI GCATC 3 cut(s) 337, 902, 907
BoxI GACNNNNGTC 1 cut(s) 771
BpiI GAAGAC 1 cut(s) 168
BpmI CTGGAG 1 cut(s) 942
BpuEI CTTGAG 1 cut(s) 524
BsaBI GATNNNNATC 2 cut(s) 212, 604
BsaWI WCCGGW 1 cut(s) 779
Bse118I RCCGGY 1 cut(s) 88
Bse1I ACTGG 1 cut(s) 114
Bse21I CCTNAGG 1 cut(s) 516
Bse8I GATNNNNATC 2 cut(s) 212, 604
BseBI CCWGG 1 cut(s) 272
BseGI GGATG 4 cut(s) 352, 627, 637, 837
BseJI GATNNNNATC 2 cut(s) 212, 604
BseMII CTCAG 5 cut(s) 195, 402, 438, 507, 587
BseNI ACTGG 1 cut(s) 114
BseRI GAGGAG 2 cut(s) 266, 602
BseX3I CGGCCG 1 cut(s) 86
BseXI GCAGC 2 cut(s) 337, 645
Bsh1285I CGRYCG 1 cut(s) 89
BshFI GGCC 3 cut(s) 88, 526, 680
BshNI GGYRCC 2 cut(s) 91, 811
BsiEI CGRYCG 1 cut(s) 89
BsiHKAI GWGCWC 2 cut(s) 594, 852
BsiHKCI CYCGRG 1 cut(s) 485
BsiSI CCGG 2 cut(s) 89, 780
BslFI GGGAC 1 cut(s) 257
BsmAI GTCTC 2 cut(s) 409, 1021
BsmFI GGGAC 1 cut(s) 257
BsnI GGCC 3 cut(s) 88, 526, 680
BsoBI CYCGRG 1 cut(s) 485
Bsp1286I GDGCHC 3 cut(s) 594, 852, 933
Bsp1407I TGTACA 1 cut(s) 834
Bsp143I GATC 4 cut(s) 74, 121, 181, 599
BspACI CCGC 2 cut(s) 179, 353
BspANI GGCC 3 cut(s) 88, 526, 680
BspCNI CTCAG 5 cut(s) 196, 403, 439, 508, 588
BspLI GGNNCC 3 cut(s) 93, 135, 813
BspPI GGATC 2 cut(s) 116, 189
BspT107I GGYRCC 2 cut(s) 91, 811
BsrFI RCCGGY 1 cut(s) 88
BsrGI TGTACA 1 cut(s) 834
BsrI ACTGG 1 cut(s) 114
BssAI RCCGGY 1 cut(s) 88
BssMI GATC 4 cut(s) 74, 121, 181, 599
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 1 cut(s) 773
Bst6I CTCTTC 2 cut(s) 87, 375
BstAUI TGTACA 1 cut(s) 834
BstC8I GCNNGC 1 cut(s) 277
BstDEI CTNAG 5 cut(s) 204, 411, 447, 516, 596
BstF5I GGATG 4 cut(s) 352, 627, 637, 837
BstKTI GATC 4 cut(s) 77, 124, 184, 602
BstMAI GTCTC 2 cut(s) 409, 1021
BstMBI GATC 4 cut(s) 74, 121, 181, 599
BstMCI CGRYCG 1 cut(s) 89
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 2 cut(s) 169, 581
BstPAI GACNNNNGTC 1 cut(s) 771
BstSCI CCNGG 1 cut(s) 270
BstV1I GCAGC 2 cut(s) 337, 645
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 2 cut(s) 181, 599
BstYI RGATCY 2 cut(s) 181, 599
BstZI CGGCCG 1 cut(s) 86
Bsu36I CCTNAGG 1 cut(s) 516
BsuRI GGCC 3 cut(s) 88, 526, 680
BtsCI GGATG 4 cut(s) 352, 627, 637, 837
Cac8I GCNNGC 1 cut(s) 277
Cfr10I RCCGGY 1 cut(s) 88
Csp6I GTAC 5 cut(s) 92, 713, 812, 835, 947
CviAII CATG 7 cut(s) 166, 311, 340, 578, 693, 797, 911
CviQI GTAC 5 cut(s) 92, 713, 812, 835, 947
DdeI CTNAG 5 cut(s) 204, 411, 447, 516, 596
DpnI GATC 4 cut(s) 76, 123, 183, 601
DpnII GATC 4 cut(s) 74, 121, 181, 599
DraI TTTAAA 1 cut(s) 9
EaeI YGGCCR 2 cut(s) 86, 524
EagI CGGCCG 1 cut(s) 86
Eam1104I CTCTTC 2 cut(s) 87, 375
EarI CTCTTC 2 cut(s) 87, 375
Ecl136II GAGCTC 1 cut(s) 592
EclXI CGGCCG 1 cut(s) 86
Eco24I GRGCYC 2 cut(s) 594, 933
Eco52I CGGCCG 1 cut(s) 86
Eco53kI GAGCTC 1 cut(s) 592
Eco81I CCTNAGG 1 cut(s) 516
Eco88I CYCGRG 1 cut(s) 485
EcoICRI GAGCTC 1 cut(s) 592
EcoRI GAATTC 1 cut(s) 477
EcoRII CCWGG 1 cut(s) 270
EcoT22I ATGCAT 1 cut(s) 895
EcoT38I GRGCYC 2 cut(s) 594, 933
FaeI CATG 7 cut(s) 169, 314, 343, 581, 696, 800, 914
FalI AAGNNNNNCTT 4 cut(s) 168, 200, 871, 903
FaqI GGGAC 1 cut(s) 257
FatI CATG 7 cut(s) 165, 310, 339, 577, 692, 796, 910
Fnu4HI GCNGC 2 cut(s) 351, 659
FokI GGATG 4 cut(s) 359, 634, 644, 844
FriOI GRGCYC 2 cut(s) 594, 933
Fsp4HI GCNGC 2 cut(s) 351, 659
FspBI CTAG 1 cut(s) 716
GluI GCNGC 2 cut(s) 351, 659
GsuI CTGGAG 1 cut(s) 942
HaeIII GGCC 3 cut(s) 88, 526, 680
HapII CCGG 2 cut(s) 89, 780
Hin1II CATG 7 cut(s) 169, 314, 343, 581, 696, 800, 914
HinfI GANTC 4 cut(s) 44, 208, 291, 962
HpaII CCGG 2 cut(s) 89, 780
Hpy188I TCNGA 5 cut(s) 205, 448, 472, 736, 972
Hpy188III TCNNGA 6 cut(s) 104, 595, 641, 653, 959, 1018
HpyAV CCTTC 6 cut(s) 166, 337, 622, 699, 794, 1004
HpyCH4III ACNGT 1 cut(s) 773
HpyCH4V TGCA 7 cut(s) 31, 279, 334, 584, 763, 893, 920
HpyF3I CTNAG 5 cut(s) 204, 411, 447, 516, 596
Hsp92II CATG 7 cut(s) 169, 314, 343, 581, 696, 800, 914
KpnI GGTACC 2 cut(s) 95, 815
Kzo9I GATC 4 cut(s) 74, 121, 181, 599
LmnI GCTCC 3 cut(s) 253, 589, 597
Lsp1109I GCAGC 2 cut(s) 337, 645
LweI GCATC 3 cut(s) 337, 902, 907
MaeI CTAG 1 cut(s) 716
MaeIII GTNAC 1 cut(s) 859
MalI GATC 4 cut(s) 76, 123, 183, 601
MboI GATC 4 cut(s) 74, 121, 181, 599
MflI RGATCY 2 cut(s) 181, 599
MhlI GDGCHC 3 cut(s) 594, 852, 933
MlsI TGGCCA 1 cut(s) 526
MluCI AATT 8 cut(s) 388, 441, 477, 665, 924, 1028, 1047, 1052
MluNI TGGCCA 1 cut(s) 526
MlyI GAGTC 1 cut(s) 971
MmeI TCCRAC 1 cut(s) 759
MnlI CCTC 6 cut(s) 244, 442, 511, 580, 672, 738
Mox20I TGGCCA 1 cut(s) 526
Mph1103I ATGCAT 1 cut(s) 895
MscI TGGCCA 1 cut(s) 526
MseI TTAA 4 cut(s) 8, 432, 792, 1005
MslI CAYNNNNRTG 3 cut(s) 17, 758, 915
Msp20I TGGCCA 1 cut(s) 526
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 2 cut(s) 89, 780
MspR9I CCNGG 1 cut(s) 272
MssI GTTTAAAC 1 cut(s) 9
MvaI CCWGG 1 cut(s) 272
NdeII GATC 4 cut(s) 74, 121, 181, 599
NlaIII CATG 7 cut(s) 169, 314, 343, 581, 696, 800, 914
NlaIV GGNNCC 3 cut(s) 93, 135, 813
NmuCI GTSAC 1 cut(s) 859
NsiI ATGCAT 1 cut(s) 895
NspI RCATGY 2 cut(s) 169, 581
OliI CACNNNNGTG 1 cut(s) 17
PciI ACATGT 1 cut(s) 577
PfeI GAWTC 3 cut(s) 44, 208, 291
PkrI GCNGC 2 cut(s) 352, 660
PleI GAGTC 1 cut(s) 970
PmeI GTTTAAAC 1 cut(s) 9
PpsI GAGTC 1 cut(s) 970
PscI ACATGT 1 cut(s) 577
PshAI GACNNNNGTC 1 cut(s) 771
Psp124BI GAGCTC 1 cut(s) 594
Psp6I CCWGG 1 cut(s) 270
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 3 cut(s) 93, 135, 813
PsrI GAACNNNNNNTAC 2 cut(s) 84, 116
PsuI RGATCY 2 cut(s) 181, 599
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 5 cut(s) 93, 714, 813, 836, 948
RsaNI GTAC 5 cut(s) 92, 713, 812, 835, 947
RseI CAYNNNNRTG 3 cut(s) 17, 758, 915
SacI GAGCTC 1 cut(s) 594
SaqAI TTAA 4 cut(s) 8, 432, 792, 1005
SatI GCNGC 2 cut(s) 351, 659
Sau3AI GATC 4 cut(s) 74, 121, 181, 599
ScaI AGTACT 1 cut(s) 714
SchI GAGTC 1 cut(s) 971
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 3 cut(s) 594, 852, 933
SfaNI GCATC 3 cut(s) 337, 902, 907
SmiMI CAYNNNNRTG 3 cut(s) 17, 758, 915
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
Sse9I AATT 8 cut(s) 388, 441, 477, 665, 924, 1028, 1047, 1052
SsiI CCGC 2 cut(s) 179, 353
SspMI CTAG 1 cut(s) 716
SstI GAGCTC 1 cut(s) 594
StyD4I CCNGG 1 cut(s) 270
TaaI ACNGT 1 cut(s) 773
TaqI TCGA 3 cut(s) 267, 502, 1019
TasI AATT 8 cut(s) 388, 441, 477, 665, 924, 1028, 1047, 1052
TatI WGTACW 2 cut(s) 712, 834
TfiI GAWTC 3 cut(s) 44, 208, 291
Tru1I TTAA 4 cut(s) 8, 432, 792, 1005
Tru9I TTAA 4 cut(s) 8, 432, 792, 1005
TseFI GTSAC 1 cut(s) 859
TseI GCWGC 2 cut(s) 350, 658
Tsp45I GTSAC 1 cut(s) 859
TspDTI ATGAA 6 cut(s) 356, 681, 813, 882, 926, 927
XapI RAATTY 4 cut(s) 388, 477, 1028, 1052
XceI RCATGY 2 cut(s) 169, 581
XspI CTAG 1 cut(s) 716
ZrmI AGTACT 1 cut(s) 714
Zsp2I ATGCAT 1 cut(s) 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.