pycom04g12020

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000451
Physical Location & Seq
Reverse (-)
14981932 .. 14986041
4110 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g12020.1

Sequence Viewer

Length: 1248 bp
ATGTTTTACAAAATATATTTACCTTACATTATTGTCTTTGACAATTATTATATCACATTAAATATCATACTCTTTTTAGTCATTTATCATATTCAAAGCATTTTATATAAAAGTTTACCAAACATTCAGACACTTTCATTATCCTCCACCAACATTGAAGCCAGCCTCTGTGAAACAAGAGTTTGGTTTTCTGATATGGAATCTGTACCAAATGTTCAAGAACTGGTAAGGAATGATCCTCTGCAGGTCCCTGAAAGTTTCCTTATCGGGAATGAGGAAGAAGAAGACAAGCTACAGAGCACTACCGACAAATATAATCCTTCTTCTGAGATCCCAATCATTGATCTTTCTCTCCTCTCGAGAGGAAGTGAGGAGGAGCTTCACAAACTGGACAAGGCTTGTGAAGAATGGGGGTTCTTTCAGGTGATAAATCATGGCGTGGCAACAGAAGTGTTGCAGGAAATGAAGGATGCTACGGCCAAGTTCTTTGAACTTCCGTTGGAAGAGAAGAACAAAATTCGTATGCCACCAGATGACTTTCAAGGCTACGGGCGAGCCTATGCGGTTACACAAGGGCAGACACTGGACTGGTCCGATGCACTGTTTCTCAGTATTTATCCAACACAGTACAGAAGGCCTAACTTATGGCCAACCGCACCAGAGGGATTCAAGGAAAATATTGAGGCATATTCAAGCGGAGTCAAAAGAATTAGAGATGAGCTCCTAAGGTGTCTATCTTCAACTCTGGGGATGGAAAAGGATGCTCTTCTTAGTCTTCATCAAGAAGTGATCCAAGTTTTGCGCGCAAACTACTATCCTCCATGCAAAATGCCTGATAAAGTACTAGGTCTTTCTCCGCACTCGGACACAAGCACCATAACCATTCTCATGCAAGAAGACAATGTAACCGGATTACAAATTCGAAAAGAAGGTGAATGGGTGCCCGTGAAGCCAATTCCAAACGCTTTCGTTGTTAATGTTGGAGATGTTATGGAGATATGGAGTAATGGGAAGTACAGGAGCATTGAACATAGAGCTGTGACAACCGAAAGCAAGTCGAGGATATCTTATGCATCATTCATTTTGCCACACGCAGAGGTGGAAGTTGAACCTTTTGGTCATATAGTGGAGTTATCTGGGTCCCAGAAGTACAAGAAGGTCAAGTACGGAGATTATCTGGGTAGTTCCTTGAAGAAGGGGAAACTTAAGGGGAAATCACACATTGAAACGGCGAAGATTGGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N Thaumatin

Protein Analysis

416

Amino Acids

47.31

Weight (kDa)

5.37

Isoelectric Point (pI)

55.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 111 - 218 6.8e-33 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 265 - 363 5.2e-35 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000451)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13280 FvH4_6g13281 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13300 FvH4_6g13322
malus_domestica MD04G1132000.v1.1 MD04G1132100.v1.1 MD04G1132400.v1.1 MD12G1145100.v1.1 MD12G1145200.v1.1
prunus_persica Prupe.6G258600_v2.0.a1 Prupe.6G258700_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258900_v2.0.a1
pyrus_communis pycom04g12010 pycom04g12020
rosa_chinensis RchiOBHm_Chr3g0466131 RchiOBHm_Chr3g0466151 RchiOBHm_Chr3g0466161 RchiOBHm_Chr3g0466171 RchiOBHm_Chr3g0466181 RchiOBHm_Chr3g0466201 RchiOBHm_Chr3g0466211 RchiOBHm_Chr6g0251841
rosa_laevigata RLG00000015425 RLG00000024596 RLG00000024599 RLG00000024600 RLG00000024601 RLG00000024602
rosa_multiflora Rmu_co8112718.1_g000001 Rmu_co8178858.1_g000001 Rmu_co8388385.1_g000001 Rmu_sc0000350.1_g000002 Rmu_sc0000350.1_g000003 Rmu_sc0000350.1_g000004 Rmu_sc0000350.1_g000006 Rmu_sc0000350.1_g000011 Rmu_sc0002249.1_g000004 Rmu_sc0007597.1_g000012 Rmu_sc0012177.1_g000002 Rmu_sc0012177.1_g000006 Rmu_sc0012177.1_g000007 Rmu_sc0022084.1_g000003 Rmu_ssc0000303.1_g000004
rosa_roxburghii Rroxscaffold_6G00414720 Rroxscaffold_6G00414730 Rroxscaffold_6G00414740 Rroxscaffold_6G00414750 Rroxscaffold_6G00414760 Rroxscaffold_6G00414770 Rroxscaffold_6G00414780 Rroxscaffold_6G00414790
rosa_rugosa Rorug03G0079600 Rorug03G0079600 Rorug03G0079800 Rorug03G0080100 Rorug03G0080200 Rorug03G0080300 Rorug03G0080400 Rorug05G0504900
rosa_samantha Rh3BG147200 Rh3BG147300 Rh3BG147400 Rh3BG147500 Rh3BG147600 Rh3CG147900 Rh3CG148000 Rh3CG148100 Rh3CG148200 Rh3CG148300 Rh3CG148500 Rh3CG148600 Rh3DG147800 Rh3DG147900 Rh3DG148100 Rh3DG148300 Rh4BG237100 Rh6AG015600 Rh6AG015700 Rh6BG013700 Rh6CG069500
rosa_wichuraiana Rw3G011870 Rw3G011880 Rw3G011890 Rw3G011900 Rw3G011910 Rw3G011930 Rw6G001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 235
AccB1I GGYRCC 1 cut(s) 940
AccII CGCG 1 cut(s) 804
AciI CCGC 4 cut(s) 563, 654, 696, 857
AclWI GGATC 3 cut(s) 230, 325, 784
AcoI YGGCCR 2 cut(s) 477, 647
AcsI RAATTY 2 cut(s) 516, 918
AfaI GTAC 6 cut(s) 207, 629, 843, 1016, 1151, 1166
AflII CTTAAG 1 cut(s) 1205
AluBI AGCT 4 cut(s) 292, 379, 721, 1037
AluI AGCT 4 cut(s) 292, 379, 721, 1037
Alw21I GWGCWC 2 cut(s) 302, 723
AlwI GGATC 3 cut(s) 230, 325, 784
AlwNI CAGNNNCTG 2 cut(s) 168, 583
Ama87I CYCGRG 1 cut(s) 358
AoxI GGCC 3 cut(s) 477, 635, 647
ApoI RAATTY 2 cut(s) 516, 918
AspLEI GCGC 2 cut(s) 804, 806
AspS9I GGNCC 3 cut(s) 247, 591, 1140
AsuHPI GGTGA 2 cut(s) 436, 944
AsuII TTCGAA 1 cut(s) 922
AvaI CYCGRG 1 cut(s) 358
AvaII GGWCC 3 cut(s) 247, 591, 1140
AxyI CCTNAGG 1 cut(s) 725
BaeGI GKGCMC 1 cut(s) 945
BalI TGGCCA 1 cut(s) 649
BanI GGYRCC 1 cut(s) 940
BanII GRGCYC 1 cut(s) 723
BarI GAAGNNNNNNTAC 4 cut(s) 276, 308, 1148, 1180
BbsI GAAGAC 3 cut(s) 291, 767, 903
Bbv12I GWGCWC 2 cut(s) 302, 723
BccI CCATC 1 cut(s) 745
BceAI ACGGC 2 cut(s) 492, 1245
BfaI CTAG 1 cut(s) 845
BfmI CTRYAG 2 cut(s) 242, 293
BfrI CTTAAG 1 cut(s) 1205
BfuAI ACCTGC 1 cut(s) 235
BmcAI AGTACT 1 cut(s) 843
Bme18I GGWCC 3 cut(s) 247, 591, 1140
BmeT110I CYCGRG 1 cut(s) 358
BmgT120I GGNCC 3 cut(s) 247, 591, 1140
BmiI GGNNCC 4 cut(s) 249, 942, 1141, 1142
BmsI GCATC 4 cut(s) 460, 586, 751, 1082
BpiI GAAGAC 3 cut(s) 291, 767, 903
BplI GAGNNNNNCTC 2 cut(s) 705, 737
Bpu14I TTCGAA 1 cut(s) 922
BsaBI GATNNNNATC 1 cut(s) 335
BsaWI WCCGGW 1 cut(s) 908
Bse1I ACTGG 4 cut(s) 228, 393, 588, 593
Bse21I CCTNAGG 1 cut(s) 725
Bse8I GATNNNNATC 1 cut(s) 335
BseGI GGATG 3 cut(s) 475, 756, 766
BseJI GATNNNNATC 1 cut(s) 335
BseMII CTCAG 2 cut(s) 318, 622
BseNI ACTGG 4 cut(s) 228, 393, 588, 593
BsePI GCGCGC 1 cut(s) 802
BseRI GAGGAG 3 cut(s) 344, 386, 389
BseSI GKGCMC 1 cut(s) 945
Bsh1236I CGCG 1 cut(s) 804
BshFI GGCC 3 cut(s) 479, 637, 649
BshNI GGYRCC 1 cut(s) 940
BsiHKAI GWGCWC 2 cut(s) 302, 723
BsiHKCI CYCGRG 1 cut(s) 358
BsiSI CCGG 1 cut(s) 909
BslFI GGGAC 2 cut(s) 233, 1126
BsmFI GGGAC 2 cut(s) 233, 1126
BsnI GGCC 3 cut(s) 479, 637, 649
BsoBI CYCGRG 1 cut(s) 358
Bsp119I TTCGAA 1 cut(s) 922
Bsp1286I GDGCHC 3 cut(s) 302, 723, 945
Bsp143I GATC 4 cut(s) 235, 330, 343, 789
BspACI CCGC 4 cut(s) 563, 654, 696, 857
BspANI GGCC 3 cut(s) 479, 637, 649
BspCNI CTCAG 2 cut(s) 319, 621
BspFNI CGCG 1 cut(s) 804
BspLI GGNNCC 4 cut(s) 249, 942, 1141, 1142
BspMAI CTGCAG 1 cut(s) 246
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 3 cut(s) 230, 325, 784
BspQI GCTCTTC 1 cut(s) 771
BspT104I TTCGAA 1 cut(s) 922
BspT107I GGYRCC 1 cut(s) 940
BspTI CTTAAG 1 cut(s) 1205
BsrI ACTGG 4 cut(s) 228, 393, 588, 593
BssHII GCGCGC 1 cut(s) 802
BssMI GATC 4 cut(s) 235, 330, 343, 789
Bst4CI ACNGT 2 cut(s) 603, 627
Bst6I CTCTTC 2 cut(s) 498, 771
BstAFI CTTAAG 1 cut(s) 1205
BstBI TTCGAA 1 cut(s) 922
BstC8I GCNNGC 3 cut(s) 163, 555, 804
BstDEI CTNAG 4 cut(s) 327, 608, 725, 770
BstF5I GGATG 3 cut(s) 475, 756, 766
BstFNI CGCG 1 cut(s) 804
BstHHI GCGC 2 cut(s) 804, 806
BstKTI GATC 4 cut(s) 238, 333, 346, 792
BstMBI GATC 4 cut(s) 235, 330, 343, 789
BstMWI GCNNNNNNNGC 1 cut(s) 949
BstSFI CTRYAG 2 cut(s) 242, 293
BstSLI GKGCMC 1 cut(s) 945
BstUI CGCG 1 cut(s) 804
BstV2I GAAGAC 3 cut(s) 291, 767, 903
BstX2I RGATCY 1 cut(s) 330
BstYI RGATCY 1 cut(s) 330
Bsu36I CCTNAGG 1 cut(s) 725
BsuRI GGCC 3 cut(s) 479, 637, 649
BtsCI GGATG 3 cut(s) 475, 756, 766
BtsIMutI CAGTG 2 cut(s) 581, 599
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 3 cut(s) 163, 555, 804
CaiI CAGNNNCTG 2 cut(s) 168, 583
CfoI GCGC 2 cut(s) 804, 806
Cfr13I GGNCC 3 cut(s) 247, 591, 1140
Csp6I GTAC 6 cut(s) 206, 628, 842, 1015, 1150, 1165
CspCI CAANNNNNGTGG 2 cut(s) 136, 171
CviAII CATG 3 cut(s) 434, 822, 889
CviQI GTAC 6 cut(s) 206, 628, 842, 1015, 1150, 1165
DdeI CTNAG 4 cut(s) 327, 608, 725, 770
DpnI GATC 4 cut(s) 237, 332, 345, 791
DpnII GATC 4 cut(s) 235, 330, 343, 789
EaeI YGGCCR 2 cut(s) 477, 647
Eam1104I CTCTTC 2 cut(s) 498, 771
EarI CTCTTC 2 cut(s) 498, 771
Ecl136II GAGCTC 1 cut(s) 721
Eco147I AGGCCT 1 cut(s) 637
Eco24I GRGCYC 1 cut(s) 723
Eco32I GATATC 1 cut(s) 1065
Eco47I GGWCC 3 cut(s) 247, 591, 1140
Eco53kI GAGCTC 1 cut(s) 721
Eco81I CCTNAGG 1 cut(s) 725
Eco88I CYCGRG 1 cut(s) 358
EcoICRI GAGCTC 1 cut(s) 721
EcoO109I RGGNCCY 2 cut(s) 247, 1140
EcoRV GATATC 1 cut(s) 1065
EcoT22I ATGCAT 1 cut(s) 1075
EcoT38I GRGCYC 1 cut(s) 723
FaeI CATG 3 cut(s) 437, 825, 892
FaqI GGGAC 2 cut(s) 233, 1126
FatI CATG 3 cut(s) 433, 821, 888
FokI GGATG 3 cut(s) 482, 763, 773
FriOI GRGCYC 1 cut(s) 723
FspBI CTAG 1 cut(s) 845
GlaI GCGC 2 cut(s) 803, 805
HaeIII GGCC 3 cut(s) 479, 637, 649
HapII CCGG 1 cut(s) 909
HhaI GCGC 2 cut(s) 804, 806
Hin1II CATG 3 cut(s) 437, 825, 892
Hin6I GCGC 2 cut(s) 802, 804
HinP1I GCGC 2 cut(s) 802, 804
HinfI GANTC 3 cut(s) 200, 666, 699
HpaII CCGG 1 cut(s) 909
HphI GGTGA 2 cut(s) 436, 944
Hpy166II GTNNAC 1 cut(s) 116
Hpy188I TCNGA 5 cut(s) 129, 193, 328, 595, 865
Hpy188III TCNNGA 5 cut(s) 218, 268, 358, 360, 782
Hpy8I GTNNAC 1 cut(s) 116
HpyAV CCTTC 6 cut(s) 330, 460, 627, 923, 1150, 1189
HpyCH4III ACNGT 2 cut(s) 603, 627
HpyCH4V TGCA 6 cut(s) 244, 457, 599, 825, 892, 1073
HpyF10VI GCNNNNNNNGC 1 cut(s) 949
HpyF3I CTNAG 4 cut(s) 327, 608, 725, 770
Hsp92II CATG 3 cut(s) 437, 825, 892
HspAI GCGC 2 cut(s) 802, 804
KflI GGGWCCC 1 cut(s) 1140
Kzo9I GATC 4 cut(s) 235, 330, 343, 789
LguI GCTCTTC 1 cut(s) 771
LmnI GCTCC 3 cut(s) 376, 726, 1020
LweI GCATC 4 cut(s) 460, 586, 751, 1082
MaeI CTAG 1 cut(s) 845
MaeIII GTNAC 3 cut(s) 565, 904, 1039
MalI GATC 4 cut(s) 237, 332, 345, 791
MboI GATC 4 cut(s) 235, 330, 343, 789
MflI RGATCY 1 cut(s) 330
MhlI GDGCHC 3 cut(s) 302, 723, 945
MlsI TGGCCA 1 cut(s) 649
MluCI AATT 5 cut(s) 43, 516, 708, 918, 954
MluNI TGGCCA 1 cut(s) 649
MlyI GAGTC 1 cut(s) 708
MmeI TCCRAC 3 cut(s) 480, 644, 961
Mox20I TGGCCA 1 cut(s) 649
Mph1103I ATGCAT 1 cut(s) 1075
MscI TGGCCA 1 cut(s) 649
MseI TTAA 3 cut(s) 59, 975, 1206
MslI CAYNNNNRTG 1 cut(s) 887
Msp20I TGGCCA 1 cut(s) 649
MspCI CTTAAG 1 cut(s) 1205
MspI CCGG 1 cut(s) 909
MvnI CGCG 1 cut(s) 804
MwoI GCNNNNNNNGC 1 cut(s) 949
NdeII GATC 4 cut(s) 235, 330, 343, 789
NlaIII CATG 3 cut(s) 437, 825, 892
NlaIV GGNNCC 4 cut(s) 249, 942, 1141, 1142
NmuCI GTSAC 1 cut(s) 1039
NsiI ATGCAT 1 cut(s) 1075
NspV TTCGAA 1 cut(s) 922
PaeR7I CTCGAG 1 cut(s) 358
PauI GCGCGC 1 cut(s) 802
PceI AGGCCT 1 cut(s) 637
PciSI GCTCTTC 1 cut(s) 771
PfeI GAWTC 2 cut(s) 200, 666
PleI GAGTC 1 cut(s) 707
PpsI GAGTC 1 cut(s) 707
PpuMI RGGWCCY 2 cut(s) 247, 1140
Psp124BI GAGCTC 1 cut(s) 723
Psp5II RGGWCCY 2 cut(s) 247, 1140
PspN4I GGNNCC 4 cut(s) 249, 942, 1141, 1142
PspPI GGNCC 3 cut(s) 247, 591, 1140
PspPPI RGGWCCY 2 cut(s) 247, 1140
PsrI GAACNNNNNNTAC 2 cut(s) 198, 230
PstI CTGCAG 1 cut(s) 246
PstNI CAGNNNCTG 2 cut(s) 168, 583
PsuI RGATCY 1 cut(s) 330
PteI GCGCGC 1 cut(s) 802
RsaI GTAC 6 cut(s) 207, 629, 843, 1016, 1151, 1166
RsaNI GTAC 6 cut(s) 206, 628, 842, 1015, 1150, 1165
RseI CAYNNNNRTG 1 cut(s) 887
SacI GAGCTC 1 cut(s) 723
SapI GCTCTTC 1 cut(s) 771
SaqAI TTAA 3 cut(s) 59, 975, 1206
Sau3AI GATC 4 cut(s) 235, 330, 343, 789
Sau96I GGNCC 3 cut(s) 247, 591, 1140
ScaI AGTACT 1 cut(s) 843
SchI GAGTC 1 cut(s) 708
SduI GDGCHC 3 cut(s) 302, 723, 945
SfaNI GCATC 4 cut(s) 460, 586, 751, 1082
SfcI CTRYAG 2 cut(s) 242, 293
Sfr274I CTCGAG 1 cut(s) 358
SfuI TTCGAA 1 cut(s) 922
SinI GGWCC 3 cut(s) 247, 591, 1140
SlaI CTCGAG 1 cut(s) 358
SmiMI CAYNNNNRTG 1 cut(s) 887
SmlI CTYRAG 2 cut(s) 358, 1205
SmoI CTYRAG 2 cut(s) 358, 1205
Sse9I AATT 5 cut(s) 43, 516, 708, 918, 954
SseBI AGGCCT 1 cut(s) 637
SsiI CCGC 4 cut(s) 563, 654, 696, 857
SspI AATATT 1 cut(s) 679
SspMI CTAG 1 cut(s) 845
SstI GAGCTC 1 cut(s) 723
StuI AGGCCT 1 cut(s) 637
TaaI ACNGT 2 cut(s) 603, 627
TaqI TCGA 3 cut(s) 359, 922, 1058
TasI AATT 5 cut(s) 43, 516, 708, 918, 954
TatI WGTACW 4 cut(s) 627, 841, 1014, 1149
TfiI GAWTC 2 cut(s) 200, 666
Tru1I TTAA 3 cut(s) 59, 975, 1206
Tru9I TTAA 3 cut(s) 59, 975, 1206
TscAI CASTG 2 cut(s) 588, 606
TseFI GTSAC 1 cut(s) 1039
Tsp45I GTSAC 1 cut(s) 1039
TspDTI ATGAA 4 cut(s) 126, 479, 767, 1069
TspGWI ACGGA 2 cut(s) 486, 1182
TspRI CASTG 2 cut(s) 588, 606
Vha464I CTTAAG 1 cut(s) 1205
VpaK11BI GGWCC 3 cut(s) 247, 591, 1140
XapI RAATTY 2 cut(s) 516, 918
XhoI CTCGAG 1 cut(s) 358
XspI CTAG 1 cut(s) 845
ZrmI AGTACT 1 cut(s) 843
Zsp2I ATGCAT 1 cut(s) 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.