Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 119 |
| Acc65I | GGTACC | 2 cut(s) | 82, 754 |
| AccB1I | GGYRCC | 2 cut(s) | 82, 754 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1219, 1281 |
| AciI | CCGC | 4 cut(s) | 299, 302, 470, 1112 |
| AclWI | GGATC | 3 cut(s) | 399, 884, 939 |
| AcoI | YGGCCR | 3 cut(s) | 376, 467, 1283 |
| AcuI | CTGAAG | 2 cut(s) | 1044, 1227 |
| AdeI | CACNNNGTG | 1 cut(s) | 265 |
| AfaI | GTAC | 7 cut(s) | 84, 449, 657, 756, 830, 971, 1265 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 79, 1219, 1281 |
| AflIII | ACRYGT | 1 cut(s) | 1036 |
| AjnI | CCWGG | 2 cut(s) | 219, 927 |
| AluBI | AGCT | 6 cut(s) | 205, 214, 288, 457, 535, 690 |
| AluI | AGCT | 6 cut(s) | 205, 214, 288, 457, 535, 690 |
| Alw21I | GWGCWC | 3 cut(s) | 207, 641, 673 |
| Alw26I | GTCTC | 2 cut(s) | 491, 549 |
| Alw44I | GTGCAC | 1 cut(s) | 669 |
| AlwI | GGATC | 3 cut(s) | 399, 884, 939 |
| Ama87I | CYCGRG | 1 cut(s) | 458 |
| AoxI | GGCC | 3 cut(s) | 376, 467, 1283 |
| ApaLI | GTGCAC | 1 cut(s) | 669 |
| Asp718I | GGTACC | 2 cut(s) | 82, 754 |
| AspS9I | GGNCC | 2 cut(s) | 411, 604 |
| AsuC2I | CCSGG | 1 cut(s) | 954 |
| AsuHPI | GGTGA | 4 cut(s) | 73, 199, 942, 1244 |
| AvaI | CYCGRG | 1 cut(s) | 458 |
| AvaII | GGWCC | 2 cut(s) | 411, 604 |
| BaeGI | GKGCMC | 3 cut(s) | 195, 673, 1191 |
| BalI | TGGCCA | 2 cut(s) | 378, 1285 |
| BanI | GGYRCC | 2 cut(s) | 82, 754 |
| BanII | GRGCYC | 1 cut(s) | 207 |
| BbsI | GAAGAC | 1 cut(s) | 1215 |
| Bbv12I | GWGCWC | 3 cut(s) | 207, 641, 673 |
| BccI | CCATC | 5 cut(s) | 170, 361, 559, 1084, 1264 |
| BceAI | ACGGC | 1 cut(s) | 1232 |
| BciT130I | CCWGG | 2 cut(s) | 221, 929 |
| BcnI | CCSGG | 1 cut(s) | 954 |
| BcoDI | GTCTC | 2 cut(s) | 491, 549 |
| BfaI | CTAG | 2 cut(s) | 215, 507 |
| BfmI | CTRYAG | 1 cut(s) | 618 |
| BfuAI | ACCTGC | 1 cut(s) | 119 |
| BisI | GCNGC | 3 cut(s) | 300, 470, 1113 |
| BlpI | GCTNAGC | 1 cut(s) | 691 |
| BlsI | GCNGC | 3 cut(s) | 301, 471, 1114 |
| BmcAI | AGTACT | 1 cut(s) | 657 |
| Bme1390I | CCNGG | 3 cut(s) | 221, 929, 954 |
| Bme18I | GGWCC | 2 cut(s) | 411, 604 |
| BmeT110I | CYCGRG | 1 cut(s) | 458 |
| BmgT120I | GGNCC | 2 cut(s) | 411, 604 |
| BmiI | GGNNCC | 3 cut(s) | 84, 159, 756 |
| BmrFI | CCNGG | 3 cut(s) | 221, 929, 954 |
| BmsI | GCATC | 3 cut(s) | 115, 565, 766 |
| BpiI | GAAGAC | 1 cut(s) | 1215 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 488, 520 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 691 |
| BpuMI | CCSGG | 1 cut(s) | 954 |
| BsaI | GGTCTC | 1 cut(s) | 549 |
| BsaJI | CCNNGG | 4 cut(s) | 927, 953, 1134, 1275 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 79, 1219, 1281 |
| Bse1I | ACTGG | 1 cut(s) | 445 |
| BseBI | CCWGG | 2 cut(s) | 221, 929 |
| BseDI | CCNNGG | 4 cut(s) | 927, 953, 1134, 1275 |
| BseGI | GGATG | 3 cut(s) | 570, 1104, 1256 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 79, 1219, 1281 |
| BseMII | CTCAG | 7 cut(s) | 27, 144, 553, 639, 647, 690, 989 |
| BseNI | ACTGG | 1 cut(s) | 445 |
| BseRI | GAGGAG | 3 cut(s) | 215, 545, 1183 |
| BseSI | GKGCMC | 3 cut(s) | 195, 673, 1191 |
| BshFI | GGCC | 3 cut(s) | 378, 469, 1285 |
| BshNI | GGYRCC | 2 cut(s) | 82, 754 |
| BsiHKAI | GWGCWC | 3 cut(s) | 207, 641, 673 |
| BsiHKCI | CYCGRG | 1 cut(s) | 458 |
| BsiSI | CCGG | 1 cut(s) | 953 |
| BslFI | GGGAC | 2 cut(s) | 18, 738 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 79, 1219, 1281 |
| BsmAI | GTCTC | 2 cut(s) | 491, 549 |
| BsmFI | GGGAC | 2 cut(s) | 18, 738 |
| BsnI | GGCC | 3 cut(s) | 378, 469, 1285 |
| Bso31I | GGTCTC | 1 cut(s) | 549 |
| BsoBI | CYCGRG | 1 cut(s) | 458 |
| Bsp1286I | GDGCHC | 5 cut(s) | 195, 207, 641, 673, 1191 |
| Bsp1407I | TGTACA | 1 cut(s) | 969 |
| Bsp143I | GATC | 4 cut(s) | 169, 391, 876, 931 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 691 |
| BspACI | CCGC | 4 cut(s) | 299, 302, 470, 1112 |
| BspANI | GGCC | 3 cut(s) | 378, 469, 1285 |
| BspCNI | CTCAG | 7 cut(s) | 26, 145, 552, 640, 646, 689, 990 |
| BspHI | TCATGA | 1 cut(s) | 1069 |
| BspLI | GGNNCC | 3 cut(s) | 84, 159, 756 |
| BspMI | ACCTGC | 1 cut(s) | 119 |
| BspPI | GGATC | 3 cut(s) | 399, 884, 939 |
| BspT107I | GGYRCC | 2 cut(s) | 82, 754 |
| BspTNI | GGTCTC | 1 cut(s) | 549 |
| BsrGI | TGTACA | 1 cut(s) | 969 |
| BsrI | ACTGG | 1 cut(s) | 445 |
| BssECI | CCNNGG | 4 cut(s) | 927, 953, 1134, 1275 |
| BssMI | GATC | 4 cut(s) | 169, 391, 876, 931 |
| BssT1I | CCWWGG | 2 cut(s) | 1134, 1275 |
| Bst2UI | CCWGG | 2 cut(s) | 221, 929 |
| Bst4CI | ACNGT | 1 cut(s) | 787 |
| Bst6I | CTCTTC | 2 cut(s) | 324, 1134 |
| BstAUI | TGTACA | 1 cut(s) | 969 |
| BstC8I | GCNNGC | 1 cut(s) | 226 |
| BstDEI | CTNAG | 9 cut(s) | 13, 153, 539, 633, 648, 665, 676, 691, 998 |
| BstF5I | GGATG | 3 cut(s) | 570, 1104, 1256 |
| BstKTI | GATC | 4 cut(s) | 172, 394, 879, 934 |
| BstMAI | GTCTC | 2 cut(s) | 491, 549 |
| BstMBI | GATC | 4 cut(s) | 169, 391, 876, 931 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 23, 211 |
| BstNI | CCWGG | 2 cut(s) | 221, 929 |
| BstNSI | RCATGY | 1 cut(s) | 1040 |
| BstSCI | CCNGG | 3 cut(s) | 219, 927, 952 |
| BstSFI | CTRYAG | 1 cut(s) | 618 |
| BstSLI | GKGCMC | 3 cut(s) | 195, 673, 1191 |
| BstV2I | GAAGAC | 1 cut(s) | 1215 |
| BstX2I | RGATCY | 1 cut(s) | 876 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1060 |
| BstYI | RGATCY | 1 cut(s) | 876 |
| BsuRI | GGCC | 3 cut(s) | 378, 469, 1285 |
| BtsCI | GGATG | 3 cut(s) | 570, 1104, 1256 |
| BtsI | GCAGTG | 1 cut(s) | 176 |
| BtsIMutI | CAGTG | 3 cut(s) | 176, 641, 783 |
| BveI | ACCTGC | 1 cut(s) | 119 |
| Cac8I | GCNNGC | 1 cut(s) | 226 |
| CciI | TCATGA | 1 cut(s) | 1069 |
| Cfr13I | GGNCC | 2 cut(s) | 411, 604 |
| CseI | GACGC | 1 cut(s) | 936 |
| Csp6I | GTAC | 7 cut(s) | 83, 448, 656, 755, 829, 970, 1264 |
| CviAII | CATG | 4 cut(s) | 30, 559, 1037, 1070 |
| CviQI | GTAC | 7 cut(s) | 83, 448, 656, 755, 829, 970, 1264 |
| DdeI | CTNAG | 9 cut(s) | 13, 153, 539, 633, 648, 665, 676, 691, 998 |
| DpnI | GATC | 4 cut(s) | 171, 393, 878, 933 |
| DpnII | GATC | 4 cut(s) | 169, 391, 876, 931 |
| DraIII | CACNNNGTG | 1 cut(s) | 265 |
| EaeI | YGGCCR | 3 cut(s) | 376, 467, 1283 |
| Eam1104I | CTCTTC | 2 cut(s) | 324, 1134 |
| EarI | CTCTTC | 2 cut(s) | 324, 1134 |
| EciI | GGCGGA | 1 cut(s) | 317 |
| Ecl136II | GAGCTC | 1 cut(s) | 205 |
| Eco130I | CCWWGG | 2 cut(s) | 1134, 1275 |
| Eco24I | GRGCYC | 1 cut(s) | 207 |
| Eco31I | GGTCTC | 1 cut(s) | 549 |
| Eco47I | GGWCC | 2 cut(s) | 411, 604 |
| Eco53kI | GAGCTC | 1 cut(s) | 205 |
| Eco57I | CTGAAG | 2 cut(s) | 1044, 1227 |
| Eco88I | CYCGRG | 1 cut(s) | 458 |
| EcoICRI | GAGCTC | 1 cut(s) | 205 |
| EcoRII | CCWGG | 2 cut(s) | 219, 927 |
| EcoT14I | CCWWGG | 2 cut(s) | 1134, 1275 |
| EcoT38I | GRGCYC | 1 cut(s) | 207 |
| ErhI | CCWWGG | 2 cut(s) | 1134, 1275 |
| FaeI | CATG | 4 cut(s) | 33, 562, 1040, 1073 |
| FaqI | GGGAC | 2 cut(s) | 18, 738 |
| FatI | CATG | 4 cut(s) | 29, 558, 1036, 1069 |
| Fnu4HI | GCNGC | 3 cut(s) | 300, 470, 1113 |
| FokI | GGATG | 3 cut(s) | 577, 1111, 1243 |
| FriOI | GRGCYC | 1 cut(s) | 207 |
| Fsp4HI | GCNGC | 3 cut(s) | 300, 470, 1113 |
| FspBI | CTAG | 2 cut(s) | 215, 507 |
| GluI | GCNGC | 3 cut(s) | 300, 470, 1113 |
| HaeIII | GGCC | 3 cut(s) | 378, 469, 1285 |
| HapII | CCGG | 1 cut(s) | 953 |
| HgaI | GACGC | 1 cut(s) | 936 |
| Hin1II | CATG | 4 cut(s) | 33, 562, 1040, 1073 |
| HinfI | GANTC | 3 cut(s) | 5, 940, 1106 |
| HpaII | CCGG | 1 cut(s) | 953 |
| HphI | GGTGA | 4 cut(s) | 73, 199, 942, 1244 |
| Hpy166II | GTNNAC | 4 cut(s) | 436, 671, 899, 1252 |
| Hpy188I | TCNGA | 9 cut(s) | 154, 396, 415, 587, 679, 999, 1024, 1207, 1231 |
| Hpy188III | TCNNGA | 5 cut(s) | 53, 100, 596, 773, 1070 |
| Hpy8I | GTNNAC | 4 cut(s) | 436, 671, 899, 1252 |
| HpyAV | CCTTC | 6 cut(s) | 391, 513, 790, 1019, 1024, 1096 |
| HpyCH4III | ACNGT | 1 cut(s) | 787 |
| HpyCH4V | TGCA | 6 cut(s) | 128, 228, 671, 706, 914, 1177 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 23, 211 |
| HpyF3I | CTNAG | 9 cut(s) | 13, 153, 539, 633, 648, 665, 676, 691, 998 |
| Hsp92II | CATG | 4 cut(s) | 33, 562, 1040, 1073 |
| KpnI | GGTACC | 2 cut(s) | 86, 758 |
| Kzo9I | GATC | 4 cut(s) | 169, 391, 876, 931 |
| LmnI | GCTCC | 3 cut(s) | 202, 532, 687 |
| LweI | GCATC | 3 cut(s) | 115, 565, 766 |
| MaeI | CTAG | 2 cut(s) | 215, 507 |
| MaeIII | GTNAC | 4 cut(s) | 277, 853, 948, 979 |
| MalI | GATC | 4 cut(s) | 171, 393, 878, 933 |
| MboI | GATC | 4 cut(s) | 169, 391, 876, 931 |
| MboII | GAAGA | 9 cut(s) | 141, 341, 346, 722, 1024, 1114, 1151, 1156, 1220 |
| MflI | RGATCY | 1 cut(s) | 876 |
| MhlI | GDGCHC | 5 cut(s) | 195, 207, 641, 673, 1191 |
| MlsI | TGGCCA | 2 cut(s) | 378, 1285 |
| MluCI | AATT | 2 cut(s) | 342, 918 |
| MluNI | TGGCCA | 2 cut(s) | 378, 1285 |
| MmeI | TCCRAC | 4 cut(s) | 438, 505, 631, 775 |
| Mox20I | TGGCCA | 2 cut(s) | 378, 1285 |
| MscI | TGGCCA | 2 cut(s) | 378, 1285 |
| MseI | TTAA | 1 cut(s) | 554 |
| MslI | CAYNNNNRTG | 4 cut(s) | 384, 563, 701, 909 |
| Msp20I | TGGCCA | 2 cut(s) | 378, 1285 |
| MspI | CCGG | 1 cut(s) | 953 |
| MspR9I | CCNGG | 3 cut(s) | 221, 929, 954 |
| MvaI | CCWGG | 2 cut(s) | 221, 929 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 23, 211 |
| NciI | CCSGG | 1 cut(s) | 954 |
| NdeII | GATC | 4 cut(s) | 169, 391, 876, 931 |
| NlaIII | CATG | 4 cut(s) | 33, 562, 1040, 1073 |
| NlaIV | GGNNCC | 3 cut(s) | 84, 159, 756 |
| NmuCI | GTSAC | 2 cut(s) | 948, 979 |
| NspI | RCATGY | 1 cut(s) | 1040 |
| PaeR7I | CTCGAG | 1 cut(s) | 458 |
| PagI | TCATGA | 1 cut(s) | 1069 |
| PciI | ACATGT | 1 cut(s) | 1036 |
| PfeI | GAWTC | 3 cut(s) | 5, 940, 1106 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1219, 1281 |
| PkrI | GCNGC | 3 cut(s) | 301, 471, 1114 |
| PscI | ACATGT | 1 cut(s) | 1036 |
| Psp124BI | GAGCTC | 1 cut(s) | 207 |
| Psp6I | CCWGG | 2 cut(s) | 219, 927 |
| PspGI | CCWGG | 2 cut(s) | 219, 927 |
| PspN4I | GGNNCC | 3 cut(s) | 84, 159, 756 |
| PspPI | GGNCC | 2 cut(s) | 411, 604 |
| PspXI | VCTCGAGB | 1 cut(s) | 458 |
| PsuI | RGATCY | 1 cut(s) | 876 |
| RsaI | GTAC | 7 cut(s) | 84, 449, 657, 756, 830, 971, 1265 |
| RsaNI | GTAC | 7 cut(s) | 83, 448, 656, 755, 829, 970, 1264 |
| RseI | CAYNNNNRTG | 4 cut(s) | 384, 563, 701, 909 |
| SacI | GAGCTC | 1 cut(s) | 207 |
| SaqAI | TTAA | 1 cut(s) | 554 |
| SatI | GCNGC | 3 cut(s) | 300, 470, 1113 |
| Sau3AI | GATC | 4 cut(s) | 169, 391, 876, 931 |
| Sau96I | GGNCC | 2 cut(s) | 411, 604 |
| ScaI | AGTACT | 1 cut(s) | 657 |
| ScrFI | CCNGG | 3 cut(s) | 221, 929, 954 |
| SduI | GDGCHC | 5 cut(s) | 195, 207, 641, 673, 1191 |
| SfaNI | GCATC | 3 cut(s) | 115, 565, 766 |
| SfcI | CTRYAG | 1 cut(s) | 618 |
| Sfr274I | CTCGAG | 1 cut(s) | 458 |
| SinI | GGWCC | 2 cut(s) | 411, 604 |
| SlaI | CTCGAG | 1 cut(s) | 458 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 384, 563, 701, 909 |
| SmlI | CTYRAG | 1 cut(s) | 458 |
| SmoI | CTYRAG | 1 cut(s) | 458 |
| Sse9I | AATT | 2 cut(s) | 342, 918 |
| SsiI | CCGC | 4 cut(s) | 299, 302, 470, 1112 |
| SspMI | CTAG | 2 cut(s) | 215, 507 |
| SstI | GAGCTC | 1 cut(s) | 207 |
| StyD4I | CCNGG | 3 cut(s) | 219, 927, 952 |
| StyI | CCWWGG | 2 cut(s) | 1134, 1275 |
| TaaI | ACNGT | 1 cut(s) | 787 |
| TaqI | TCGA | 1 cut(s) | 459 |
| TasI | AATT | 2 cut(s) | 342, 918 |
| TatI | WGTACW | 5 cut(s) | 447, 655, 828, 969, 1263 |
| TauI | GCSGC | 3 cut(s) | 302, 472, 1115 |
| TfiI | GAWTC | 3 cut(s) | 5, 940, 1106 |
| Tru1I | TTAA | 1 cut(s) | 554 |
| Tru9I | TTAA | 1 cut(s) | 554 |
| TscAI | CASTG | 3 cut(s) | 183, 641, 790 |
| TseFI | GTSAC | 2 cut(s) | 948, 979 |
| Tsp45I | GTSAC | 2 cut(s) | 948, 979 |
| TspDTI | ATGAA | 5 cut(s) | 120, 1031, 1086, 1115, 1185 |
| TspGWI | ACGGA | 3 cut(s) | 276, 759, 954 |
| TspRI | CASTG | 3 cut(s) | 183, 641, 790 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1219, 1281 |
| VneI | GTGCAC | 1 cut(s) | 669 |
| VpaK11BI | GGWCC | 2 cut(s) | 411, 604 |
| XceI | RCATGY | 1 cut(s) | 1040 |
| XhoI | CTCGAG | 1 cut(s) | 458 |
| XspI | CTAG | 2 cut(s) | 215, 507 |
| ZrmI | AGTACT | 1 cut(s) | 657 |