MD12G1145100.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000451
Physical Location & Seq
Reverse (-)
22420455 .. 22431651
11197 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1145100.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGGCATTTGCACCAAATGTTCAAGAAATGGTAAGAAGTAACCCATTGCAGGTCCCTGAAACATTCCTCGTAAGCAGGAAGGAAGAAGACGAGCCAAAGAGTACGGCAGACGTGCTTGATCTTTCATCGAAGATCCCTATCCTCGATCTTTCTATGCTCTCGAGAGAGCACAAGGAGGAGCTCAACAAACTGGACCAAGCTTGCAAAGAATGGGGATTCTTTCAGGTGGTTAATCATGGAGTGGCAACGGAATTGCTGCAGGAGATGAAGGATGCAACGGCCAAGTTCTTTGAACTTCCTCTAGAAGAGAAGAATAAAATACGTATGTCAGGTGGCCGCGAGGGCTATGGGCAAGCCTATGCGATTTCCGAAGGGCAGACAATGGACTGGTCCGACACACTAATTCTAAGCCTTTATCCAGCTCAGTCAAGAGACCTTCAGTTTTGGCCAACTGCACCAAATGGATTCAGGGAAACTATCGAGGCATATTCAAGTGAAGTTAAAAGAATTGGGGAGGAACTCATAAGGTCTCTATCTACTATCATGGAGCTAGAGAAGGATGCTCTTCTTGGACTGCATAAAGAGGTGCTTCAAGCCTTGCGGGTGAACTACACTCCTCCATGCTCCATGCCTGATAAAGTACTAGCTCTAAGTCCGCACTCGGATACAACCACCATAACCATACTCATGCAAGAAGATAATGTCACCGGATTACAGATTCGAAAAGAAGGCAAATGGGTGCCGGTGAAGCCGATTCCGAACGCTCTCGTTGTGAATGTCGGAGATGCTCTTGAGATATGGAGCAATGGGAAGTACAAGAGCATTGAGCATAGAGCTGTCCCCAACGAAAGCAGGCTGAGGATATCTTATGCATCATTTCTTTTTCCACATACTGACGCGGAAGTTGGACCGTTTGATCATATGGTGGAGTCATCAAGAATATACAAGAAATTAAAATTTGGAGATTATATGGCGAAGGCAATAAAGGGGAAACTCGATGGGAAGGCACACACTGAAATGGCAAAGATTGGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N Thaumatin

Protein Analysis

346

Amino Acids

38.87

Weight (kDa)

5.62

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 45 - 151 2.8e-30 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 198 - 296 5.3e-32 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000451)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13280 FvH4_6g13281 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13300 FvH4_6g13322
malus_domestica MD04G1132000.v1.1 MD04G1132100.v1.1 MD04G1132400.v1.1 MD12G1145100.v1.1 MD12G1145200.v1.1
prunus_persica Prupe.6G258600_v2.0.a1 Prupe.6G258700_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258900_v2.0.a1
pyrus_communis pycom04g12010 pycom04g12020
rosa_chinensis RchiOBHm_Chr3g0466131 RchiOBHm_Chr3g0466151 RchiOBHm_Chr3g0466161 RchiOBHm_Chr3g0466171 RchiOBHm_Chr3g0466181 RchiOBHm_Chr3g0466201 RchiOBHm_Chr3g0466211 RchiOBHm_Chr6g0251841
rosa_laevigata RLG00000015425 RLG00000024596 RLG00000024599 RLG00000024600 RLG00000024601 RLG00000024602
rosa_multiflora Rmu_co8112718.1_g000001 Rmu_co8178858.1_g000001 Rmu_co8388385.1_g000001 Rmu_sc0000350.1_g000002 Rmu_sc0000350.1_g000003 Rmu_sc0000350.1_g000004 Rmu_sc0000350.1_g000006 Rmu_sc0000350.1_g000011 Rmu_sc0002249.1_g000004 Rmu_sc0007597.1_g000012 Rmu_sc0012177.1_g000002 Rmu_sc0012177.1_g000006 Rmu_sc0012177.1_g000007 Rmu_sc0022084.1_g000003 Rmu_ssc0000303.1_g000004
rosa_roxburghii Rroxscaffold_6G00414720 Rroxscaffold_6G00414730 Rroxscaffold_6G00414740 Rroxscaffold_6G00414750 Rroxscaffold_6G00414760 Rroxscaffold_6G00414770 Rroxscaffold_6G00414780 Rroxscaffold_6G00414790
rosa_rugosa Rorug03G0079600 Rorug03G0079600 Rorug03G0079800 Rorug03G0080100 Rorug03G0080200 Rorug03G0080300 Rorug03G0080400 Rorug05G0504900
rosa_samantha Rh3BG147200 Rh3BG147300 Rh3BG147400 Rh3BG147500 Rh3BG147600 Rh3CG147900 Rh3CG148000 Rh3CG148100 Rh3CG148200 Rh3CG148300 Rh3CG148500 Rh3CG148600 Rh3DG147800 Rh3DG147900 Rh3DG148100 Rh3DG148300 Rh4BG237100 Rh6AG015600 Rh6AG015700 Rh6BG013700 Rh6CG069500
rosa_wichuraiana Rw3G011870 Rw3G011880 Rw3G011890 Rw3G011900 Rw3G011910 Rw3G011930 Rw6G001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 40
AccB1I GGYRCC 1 cut(s) 739
AccII CGCG 2 cut(s) 339, 899
AciI CCGC 4 cut(s) 337, 601, 656, 899
AclWI GGATC 1 cut(s) 127
AcoI YGGCCR 3 cut(s) 279, 334, 446
AcsI RAATTY 1 cut(s) 956
AcuI CTGAAG 1 cut(s) 422
AfaI GTAC 3 cut(s) 103, 642, 815
AfiI CCNNNNNNNGG 1 cut(s) 49
AgsI TTSAA 4 cut(s) 23, 293, 492, 593
AjiI CACGTC 1 cut(s) 112
AluBI AGCT 6 cut(s) 181, 200, 422, 550, 647, 836
AluI AGCT 6 cut(s) 181, 200, 422, 550, 647, 836
Alw21I GWGCWC 2 cut(s) 171, 183
Alw26I GTCTC 2 cut(s) 426, 534
AlwI GGATC 1 cut(s) 127
Ama87I CYCGRG 1 cut(s) 160
AoxI GGCC 3 cut(s) 279, 334, 446
ApeKI GCWGC 1 cut(s) 256
ApoI RAATTY 1 cut(s) 956
AspS9I GGNCC 4 cut(s) 52, 193, 390, 908
AsuHPI GGTGA 3 cut(s) 616, 697, 757
AsuII TTCGAA 1 cut(s) 721
AvaI CYCGRG 1 cut(s) 160
AvaII GGWCC 4 cut(s) 52, 193, 390, 908
BalI TGGCCA 1 cut(s) 448
BanI GGYRCC 1 cut(s) 739
BanII GRGCYC 1 cut(s) 183
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 2 cut(s) 171, 183
BbvCI CCTCAGC 1 cut(s) 857
BbvI GCAGC 1 cut(s) 243
BccI CCATC 1 cut(s) 992
BceAI ACGGC 2 cut(s) 120, 294
BciVI GTATCC 1 cut(s) 658
BclI TGATCA 1 cut(s) 916
BcoDI GTCTC 2 cut(s) 426, 534
BfaI CTAG 3 cut(s) 302, 551, 644
BfmI CTRYAG 1 cut(s) 257
BfuAI ACCTGC 1 cut(s) 40
BfuI GTATCC 1 cut(s) 658
BglI GCCNNNNNGGC 1 cut(s) 342
BisI GCNGC 2 cut(s) 257, 337
BlsI GCNGC 2 cut(s) 258, 338
BmcAI AGTACT 1 cut(s) 642
Bme18I GGWCC 4 cut(s) 52, 193, 390, 908
BmeT110I CYCGRG 1 cut(s) 160
BmgBI CACGTC 1 cut(s) 112
BmgT120I GGNCC 4 cut(s) 52, 193, 390, 908
BmiI GGNNCC 2 cut(s) 54, 741
BmsI GCATC 4 cut(s) 262, 550, 775, 881
BpiI GAAGAC 1 cut(s) 93
Bpu10I CCTNAGC 1 cut(s) 857
Bpu14I TTCGAA 1 cut(s) 721
BpuEI CTTGAG 1 cut(s) 812
BsaAI YACGTR 1 cut(s) 323
BsaBI GATNNNNATC 1 cut(s) 137
BsaI GGTCTC 2 cut(s) 426, 534
BsaWI WCCGGW 1 cut(s) 707
Bsc4I CCNNNNNNNGG 1 cut(s) 49
Bse118I RCCGGY 1 cut(s) 742
Bse1I ACTGG 2 cut(s) 195, 392
Bse3DI GCAATG 2 cut(s) 44, 811
Bse8I GATNNNNATC 1 cut(s) 137
BseGI GGATG 2 cut(s) 277, 565
BseJI GATNNNNATC 1 cut(s) 137
BseLI CCNNNNNNNGG 1 cut(s) 49
BseMI GCAATG 2 cut(s) 44, 811
BseMII CTCAG 2 cut(s) 437, 848
BseNI ACTGG 2 cut(s) 195, 392
BseRI GAGGAG 2 cut(s) 191, 606
BseXI GCAGC 1 cut(s) 243
BsgI GTGCAG 1 cut(s) 438
Bsh1236I CGCG 2 cut(s) 339, 899
BshFI GGCC 3 cut(s) 281, 336, 448
BshNI GGYRCC 1 cut(s) 739
BsiHKAI GWGCWC 2 cut(s) 171, 183
BsiHKCI CYCGRG 1 cut(s) 160
BsiSI CCGG 2 cut(s) 708, 743
BslFI GGGAC 2 cut(s) 38, 824
BslI CCNNNNNNNGG 1 cut(s) 49
BsmAI GTCTC 2 cut(s) 426, 534
BsmFI GGGAC 2 cut(s) 38, 824
BsnI GGCC 3 cut(s) 281, 336, 448
Bso31I GGTCTC 2 cut(s) 426, 534
BsoBI CYCGRG 1 cut(s) 160
Bsp119I TTCGAA 1 cut(s) 721
Bsp1286I GDGCHC 2 cut(s) 171, 183
Bsp143I GATC 4 cut(s) 118, 132, 145, 916
BspACI CCGC 4 cut(s) 337, 601, 656, 899
BspANI GGCC 3 cut(s) 281, 336, 448
BspCNI CTCAG 2 cut(s) 436, 849
BspFNI CGCG 2 cut(s) 339, 899
BspLI GGNNCC 2 cut(s) 54, 741
BspMAI CTGCAG 1 cut(s) 261
BspMI ACCTGC 1 cut(s) 40
BspPI GGATC 1 cut(s) 127
BspQI GCTCTTC 1 cut(s) 570
BspT104I TTCGAA 1 cut(s) 721
BspT107I GGYRCC 1 cut(s) 739
BspTNI GGTCTC 2 cut(s) 426, 534
BsrDI GCAATG 2 cut(s) 44, 811
BsrFI RCCGGY 1 cut(s) 742
BsrI ACTGG 2 cut(s) 195, 392
BssAI RCCGGY 1 cut(s) 742
BssMI GATC 4 cut(s) 118, 132, 145, 916
Bst4CI ACNGT 1 cut(s) 912
Bst6I CTCTTC 2 cut(s) 300, 570
BstBAI YACGTR 1 cut(s) 323
BstBI TTCGAA 1 cut(s) 721
BstC8I GCNNGC 3 cut(s) 202, 354, 854
BstDEI CTNAG 4 cut(s) 407, 423, 650, 857
BstF5I GGATG 2 cut(s) 277, 565
BstFNI CGCG 2 cut(s) 339, 899
BstKTI GATC 4 cut(s) 121, 135, 148, 919
BstMAI GTCTC 2 cut(s) 426, 534
BstMBI GATC 4 cut(s) 118, 132, 145, 916
BstMWI GCNNNNNNNGC 2 cut(s) 342, 748
BstSFI CTRYAG 1 cut(s) 257
BstSNI TACGTA 1 cut(s) 323
BstUI CGCG 2 cut(s) 339, 899
BstV1I GCAGC 1 cut(s) 243
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 1 cut(s) 132
BstYI RGATCY 1 cut(s) 132
BsuI GTATCC 1 cut(s) 658
BsuRI GGCC 3 cut(s) 281, 336, 448
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 2 cut(s) 277, 565
BtsIMutI CAGTG 1 cut(s) 1011
BveI ACCTGC 1 cut(s) 40
Cac8I GCNNGC 3 cut(s) 202, 354, 854
Cfr10I RCCGGY 1 cut(s) 742
Cfr13I GGNCC 4 cut(s) 52, 193, 390, 908
CseI GACGC 1 cut(s) 905
Csp6I GTAC 3 cut(s) 102, 641, 814
CviAII CATG 5 cut(s) 236, 544, 621, 628, 688
CviQI GTAC 3 cut(s) 102, 641, 814
DdeI CTNAG 4 cut(s) 407, 423, 650, 857
DpnI GATC 4 cut(s) 120, 134, 147, 918
DpnII GATC 4 cut(s) 118, 132, 145, 916
EaeI YGGCCR 3 cut(s) 279, 334, 446
Eam1104I CTCTTC 2 cut(s) 300, 570
EarI CTCTTC 2 cut(s) 300, 570
Ecl136II GAGCTC 1 cut(s) 181
Eco105I TACGTA 1 cut(s) 323
Eco24I GRGCYC 1 cut(s) 183
Eco31I GGTCTC 2 cut(s) 426, 534
Eco32I GATATC 1 cut(s) 864
Eco47I GGWCC 4 cut(s) 52, 193, 390, 908
Eco53kI GAGCTC 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 422
Eco88I CYCGRG 1 cut(s) 160
EcoICRI GAGCTC 1 cut(s) 181
EcoO109I RGGNCCY 1 cut(s) 52
EcoRV GATATC 1 cut(s) 864
EcoT22I ATGCAT 1 cut(s) 874
EcoT38I GRGCYC 1 cut(s) 183
FaeI CATG 5 cut(s) 239, 547, 624, 631, 691
FalI AAGNNNNNCTT 2 cut(s) 573, 605
FaqI GGGAC 2 cut(s) 38, 824
FatI CATG 5 cut(s) 235, 543, 620, 627, 687
FauI CCCGC 1 cut(s) 594
FauNDI CATATG 1 cut(s) 921
FbaI TGATCA 1 cut(s) 916
Fnu4HI GCNGC 2 cut(s) 257, 337
FokI GGATG 2 cut(s) 284, 572
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 2 cut(s) 257, 337
FspBI CTAG 3 cut(s) 302, 551, 644
GluI GCNGC 2 cut(s) 257, 337
HaeIII GGCC 3 cut(s) 281, 336, 448
HapII CCGG 2 cut(s) 708, 743
HgaI GACGC 1 cut(s) 905
Hin1II CATG 5 cut(s) 239, 547, 624, 631, 691
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 5 cut(s) 216, 465, 718, 754, 929
HpaII CCGG 2 cut(s) 708, 743
HphI GGTGA 3 cut(s) 616, 697, 757
Hpy166II GTNNAC 1 cut(s) 607
Hpy188I TCNGA 5 cut(s) 370, 394, 664, 759, 782
Hpy188III TCNNGA 7 cut(s) 23, 160, 162, 302, 429, 791, 936
Hpy8I GTNNAC 1 cut(s) 607
HpyAV CCTTC 8 cut(s) 73, 262, 365, 446, 550, 722, 970, 997
HpyCH4III ACNGT 1 cut(s) 912
HpyCH4IV ACGT 2 cut(s) 111, 322
HpyCH4V TGCA 9 cut(s) 11, 49, 204, 259, 275, 455, 577, 691, 872
HpyF10VI GCNNNNNNNGC 2 cut(s) 342, 748
HpyF3I CTNAG 4 cut(s) 407, 423, 650, 857
HpySE526I ACGT 2 cut(s) 111, 322
Hsp92II CATG 5 cut(s) 239, 547, 624, 631, 691
Ksp22I TGATCA 1 cut(s) 916
Kzo9I GATC 4 cut(s) 118, 132, 145, 916
LguI GCTCTTC 1 cut(s) 570
LmnI GCTCC 4 cut(s) 178, 547, 629, 801
Lsp1109I GCAGC 1 cut(s) 243
LweI GCATC 4 cut(s) 262, 550, 775, 881
MaeI CTAG 3 cut(s) 302, 551, 644
MaeII ACGT 2 cut(s) 111, 322
MaeIII GTNAC 2 cut(s) 38, 703
MalI GATC 4 cut(s) 120, 134, 147, 918
MboI GATC 4 cut(s) 118, 132, 145, 916
MboII GAAGA 7 cut(s) 95, 98, 142, 317, 322, 557, 707
MflI RGATCY 1 cut(s) 132
MhlI GDGCHC 2 cut(s) 171, 183
MlsI TGGCCA 1 cut(s) 448
MluCI AATT 5 cut(s) 251, 402, 507, 950, 956
MluNI TGGCCA 1 cut(s) 448
MlyI GAGTC 1 cut(s) 938
MmeI TCCRAC 3 cut(s) 417, 760, 886
Mox20I TGGCCA 1 cut(s) 448
Mph1103I ATGCAT 1 cut(s) 874
MscI TGGCCA 1 cut(s) 448
MseI TTAA 3 cut(s) 231, 501, 953
MslI CAYNNNNRTG 2 cut(s) 686, 1016
Msp20I TGGCCA 1 cut(s) 448
MspI CCGG 2 cut(s) 708, 743
MvnI CGCG 2 cut(s) 339, 899
MwoI GCNNNNNNNGC 2 cut(s) 342, 748
NdeI CATATG 1 cut(s) 921
NdeII GATC 4 cut(s) 118, 132, 145, 916
NlaIII CATG 5 cut(s) 239, 547, 624, 631, 691
NlaIV GGNNCC 2 cut(s) 54, 741
NmuCI GTSAC 1 cut(s) 703
NsiI ATGCAT 1 cut(s) 874
NspV TTCGAA 1 cut(s) 721
PaeR7I CTCGAG 1 cut(s) 160
PciSI GCTCTTC 1 cut(s) 570
PfeI GAWTC 4 cut(s) 216, 465, 718, 754
PkrI GCNGC 2 cut(s) 258, 338
PleI GAGTC 1 cut(s) 937
PpsI GAGTC 1 cut(s) 937
Ppu21I YACGTR 1 cut(s) 323
PpuMI RGGWCCY 1 cut(s) 52
Psp124BI GAGCTC 1 cut(s) 183
Psp5II RGGWCCY 1 cut(s) 52
PspN4I GGNNCC 2 cut(s) 54, 741
PspPI GGNCC 4 cut(s) 52, 193, 390, 908
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 1 cut(s) 261
PsuI RGATCY 1 cut(s) 132
RsaI GTAC 3 cut(s) 103, 642, 815
RsaNI GTAC 3 cut(s) 102, 641, 814
RseI CAYNNNNRTG 2 cut(s) 686, 1016
SacI GAGCTC 1 cut(s) 183
SapI GCTCTTC 1 cut(s) 570
SaqAI TTAA 3 cut(s) 231, 501, 953
SatI GCNGC 2 cut(s) 257, 337
Sau3AI GATC 4 cut(s) 118, 132, 145, 916
Sau96I GGNCC 4 cut(s) 52, 193, 390, 908
ScaI AGTACT 1 cut(s) 642
SchI GAGTC 1 cut(s) 938
SduI GDGCHC 2 cut(s) 171, 183
SfaNI GCATC 4 cut(s) 262, 550, 775, 881
SfcI CTRYAG 1 cut(s) 257
Sfr274I CTCGAG 1 cut(s) 160
SfuI TTCGAA 1 cut(s) 721
SinI GGWCC 4 cut(s) 52, 193, 390, 908
SlaI CTCGAG 1 cut(s) 160
SmiMI CAYNNNNRTG 2 cut(s) 686, 1016
SmlI CTYRAG 2 cut(s) 160, 791
SmoI CTYRAG 2 cut(s) 160, 791
SnaBI TACGTA 1 cut(s) 323
Sse9I AATT 5 cut(s) 251, 402, 507, 950, 956
SsiI CCGC 4 cut(s) 337, 601, 656, 899
SspMI CTAG 3 cut(s) 302, 551, 644
SstI GAGCTC 1 cut(s) 183
TaaI ACNGT 1 cut(s) 912
TaiI ACGT 2 cut(s) 114, 325
TaqI TCGA 6 cut(s) 128, 144, 161, 480, 721, 996
TasI AATT 5 cut(s) 251, 402, 507, 950, 956
TatI WGTACW 2 cut(s) 640, 813
TauI GCSGC 1 cut(s) 339
TfiI GAWTC 4 cut(s) 216, 465, 718, 754
Tru1I TTAA 3 cut(s) 231, 501, 953
Tru9I TTAA 3 cut(s) 231, 501, 953
TscAI CASTG 1 cut(s) 1018
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 1 cut(s) 256
Tsp45I GTSAC 1 cut(s) 703
TspDTI ATGAA 2 cut(s) 114, 281
TspGWI ACGGA 1 cut(s) 263
TspRI CASTG 1 cut(s) 1018
VpaK11BI GGWCC 4 cut(s) 52, 193, 390, 908
XapI RAATTY 1 cut(s) 956
XbaI TCTAGA 1 cut(s) 301
XhoI CTCGAG 1 cut(s) 160
XspI CTAG 3 cut(s) 302, 551, 644
ZrmI AGTACT 1 cut(s) 642
Zsp2I ATGCAT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.