MD04G1132400.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000451
Physical Location & Seq
Reverse (-)
21908063 .. 21912677
4615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1132400.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGGAATCTGTACCAAATGTTCAAGAACTGGTAAGAAATGATCCTCTGCAGGTCCCTGAAAGTTTCCTTATCGGGAATGAGGAAGAAGAAGACAAGCTACAGAGTACTGCAGACAAATATAATCTTTCTTCTGAGATCCCAATCATTGATCTTTCTCTCCTCTCGAGAGGAAGTGAGGAGGAGCTTCACAAACTGGACAAGGCTTGTGAAGAATGGGGGTTCTTTCAGGTGATAAATCATGGAGTGGCAACAGAAGTGTTGCAGGAAATGAAGGATGCCACGACCAAGTTCTTTGAACTTCCGTTGGAAGAGAAGAACAAAATTCGTATGCCACCGGATGACTTTCAAGGCTACGGCCAAGCCTATGCGGTTGCACAAGGGCAGACACTGGACTGGTCTGATGCACTGTTTCTCAGGATTTATCCAGCACAGTACAGAAAGCCTAACTTATGGCCAATCGCACCAGAGGGATTCAAGGAAACTATTGAGGCGTATTCAAGCGGAGTTAAAAGAATTAGAGATGAGCTCCTAAGGTGTCTATCTTCAACTCTGGGGATGGAAAAGGATGCTCTTCTTAATCTTCATCAAGAAGTAACCCAAGCTTTGCGCGTAAACTACTATCCTCCATGCAACATGCCTGGTAAAGTACTAGGTCTTAGTCCGCACTCGGACACAAGCACCATAACCATTCTCATGCAAGAAGACAATGTAACCGGATTACAAATTCGAAAAGAAGGTGAATGGGTGCCCGTGAAGCCAATTCCAAACGCTCTCGTTGTTAATGTTGGAGATGTTATGGAGATATGGAGTAATGGGAAGTACAGGAGCATTGAACATAGAGCTGTGACAACCGAAAGCAAATCGAGGATATCTTATGCATCATTCATTTTGCCACACGCAGAGGTGGAAGTTGAACCTTTTGGTCATATAGTGGAGTTATCTGGGTCCCAGAAGTACAAGAAGGTCAAGTACGGAGATTATCTGCGTAGTTCCTTGAAGGGGAAACTTAAGGGGAAATCACACATTGAAACGGCGAAGATTGGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N Thaumatin

Protein Analysis

350

Amino Acids

39.41

Weight (kDa)

5.36

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 46 - 152 2.4e-32 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 200 - 298 2.2e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000451)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13280 FvH4_6g13281 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13300 FvH4_6g13322
malus_domestica MD04G1132000.v1.1 MD04G1132100.v1.1 MD04G1132400.v1.1 MD12G1145100.v1.1 MD12G1145200.v1.1
prunus_persica Prupe.6G258600_v2.0.a1 Prupe.6G258700_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258900_v2.0.a1
pyrus_communis pycom04g12010 pycom04g12020
rosa_chinensis RchiOBHm_Chr3g0466131 RchiOBHm_Chr3g0466151 RchiOBHm_Chr3g0466161 RchiOBHm_Chr3g0466171 RchiOBHm_Chr3g0466181 RchiOBHm_Chr3g0466201 RchiOBHm_Chr3g0466211 RchiOBHm_Chr6g0251841
rosa_laevigata RLG00000015425 RLG00000024596 RLG00000024599 RLG00000024600 RLG00000024601 RLG00000024602
rosa_multiflora Rmu_co8112718.1_g000001 Rmu_co8178858.1_g000001 Rmu_co8388385.1_g000001 Rmu_sc0000350.1_g000002 Rmu_sc0000350.1_g000003 Rmu_sc0000350.1_g000004 Rmu_sc0000350.1_g000006 Rmu_sc0000350.1_g000011 Rmu_sc0002249.1_g000004 Rmu_sc0007597.1_g000012 Rmu_sc0012177.1_g000002 Rmu_sc0012177.1_g000006 Rmu_sc0012177.1_g000007 Rmu_sc0022084.1_g000003 Rmu_ssc0000303.1_g000004
rosa_roxburghii Rroxscaffold_6G00414720 Rroxscaffold_6G00414730 Rroxscaffold_6G00414740 Rroxscaffold_6G00414750 Rroxscaffold_6G00414760 Rroxscaffold_6G00414770 Rroxscaffold_6G00414780 Rroxscaffold_6G00414790
rosa_rugosa Rorug03G0079600 Rorug03G0079600 Rorug03G0079800 Rorug03G0080100 Rorug03G0080200 Rorug03G0080300 Rorug03G0080400 Rorug05G0504900
rosa_samantha Rh3BG147200 Rh3BG147300 Rh3BG147400 Rh3BG147500 Rh3BG147600 Rh3CG147900 Rh3CG148000 Rh3CG148100 Rh3CG148200 Rh3CG148300 Rh3CG148500 Rh3CG148600 Rh3DG147800 Rh3DG147900 Rh3DG148100 Rh3DG148300 Rh4BG237100 Rh6AG015600 Rh6AG015700 Rh6BG013700 Rh6CG069500
rosa_wichuraiana Rw3G011870 Rw3G011880 Rw3G011890 Rw3G011900 Rw3G011910 Rw3G011930 Rw6G001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 40
AccB1I GGYRCC 1 cut(s) 745
AccII CGCG 1 cut(s) 609
AciI CCGC 3 cut(s) 368, 501, 662
AclWI GGATC 2 cut(s) 35, 130
AcoI YGGCCR 2 cut(s) 355, 452
AcsI RAATTY 2 cut(s) 321, 723
AfaI GTAC 7 cut(s) 12, 106, 434, 648, 821, 956, 971
AfiI CCNNNNNNNGG 1 cut(s) 999
AflII CTTAAG 1 cut(s) 1007
AjnI CCWGG 1 cut(s) 637
AluBI AGCT 5 cut(s) 97, 184, 526, 602, 842
AluI AGCT 5 cut(s) 97, 184, 526, 602, 842
Alw21I GWGCWC 1 cut(s) 528
AlwI GGATC 2 cut(s) 35, 130
AlwNI CAGNNNCTG 1 cut(s) 388
Ama87I CYCGRG 1 cut(s) 163
AoxI GGCC 2 cut(s) 355, 452
ApoI RAATTY 2 cut(s) 321, 723
AspLEI GCGC 1 cut(s) 609
AspS9I GGNCC 2 cut(s) 52, 945
AsuHPI GGTGA 2 cut(s) 241, 749
AsuII TTCGAA 1 cut(s) 727
AvaI CYCGRG 1 cut(s) 163
AvaII GGWCC 2 cut(s) 52, 945
AxyI CCTNAGG 1 cut(s) 530
BaeGI GKGCMC 1 cut(s) 750
BalI TGGCCA 1 cut(s) 454
BanI GGYRCC 1 cut(s) 745
BanII GRGCYC 1 cut(s) 528
BarI GAAGNNNNNNTAC 4 cut(s) 81, 113, 953, 985
BbsI GAAGAC 2 cut(s) 96, 708
Bbv12I GWGCWC 1 cut(s) 528
BccI CCATC 1 cut(s) 550
BceAI ACGGC 2 cut(s) 370, 1047
BciT130I CCWGG 1 cut(s) 639
BfaI CTAG 1 cut(s) 650
BfmI CTRYAG 3 cut(s) 47, 98, 108
BfrI CTTAAG 1 cut(s) 1007
BfuAI ACCTGC 1 cut(s) 40
BmcAI AGTACT 2 cut(s) 106, 648
Bme1390I CCNGG 1 cut(s) 639
Bme18I GGWCC 2 cut(s) 52, 945
BmeT110I CYCGRG 1 cut(s) 163
BmgT120I GGNCC 2 cut(s) 52, 945
BmiI GGNNCC 4 cut(s) 54, 747, 946, 947
BmrFI CCNGG 1 cut(s) 639
BmsI GCATC 4 cut(s) 265, 391, 556, 887
BpiI GAAGAC 2 cut(s) 96, 708
BplI GAGNNNNNCTC 2 cut(s) 510, 542
Bpu14I TTCGAA 1 cut(s) 727
BsaBI GATNNNNATC 1 cut(s) 140
BsaWI WCCGGW 2 cut(s) 334, 713
Bsc4I CCNNNNNNNGG 1 cut(s) 999
Bse1I ACTGG 4 cut(s) 33, 198, 393, 398
Bse21I CCTNAGG 1 cut(s) 530
Bse8I GATNNNNATC 1 cut(s) 140
BseBI CCWGG 1 cut(s) 639
BseGI GGATG 4 cut(s) 280, 343, 561, 571
BseJI GATNNNNATC 1 cut(s) 140
BseLI CCNNNNNNNGG 1 cut(s) 999
BseMII CTCAG 2 cut(s) 123, 427
BseNI ACTGG 4 cut(s) 33, 198, 393, 398
BseRI GAGGAG 3 cut(s) 149, 191, 194
BseSI GKGCMC 1 cut(s) 750
Bsh1236I CGCG 1 cut(s) 609
BshFI GGCC 2 cut(s) 357, 454
BshNI GGYRCC 1 cut(s) 745
BsiHKAI GWGCWC 1 cut(s) 528
BsiHKCI CYCGRG 1 cut(s) 163
BsiSI CCGG 2 cut(s) 335, 714
BslFI GGGAC 2 cut(s) 38, 931
BslI CCNNNNNNNGG 1 cut(s) 999
BsmFI GGGAC 2 cut(s) 38, 931
BsnI GGCC 2 cut(s) 357, 454
BsoBI CYCGRG 1 cut(s) 163
Bsp119I TTCGAA 1 cut(s) 727
Bsp1286I GDGCHC 2 cut(s) 528, 750
Bsp143I GATC 3 cut(s) 40, 135, 148
BspACI CCGC 3 cut(s) 368, 501, 662
BspANI GGCC 2 cut(s) 357, 454
BspCNI CTCAG 2 cut(s) 124, 426
BspFNI CGCG 1 cut(s) 609
BspLI GGNNCC 4 cut(s) 54, 747, 946, 947
BspMAI CTGCAG 2 cut(s) 51, 112
BspMI ACCTGC 1 cut(s) 40
BspPI GGATC 2 cut(s) 35, 130
BspQI GCTCTTC 1 cut(s) 576
BspT104I TTCGAA 1 cut(s) 727
BspT107I GGYRCC 1 cut(s) 745
BspTI CTTAAG 1 cut(s) 1007
BsrI ACTGG 4 cut(s) 33, 198, 393, 398
BssMI GATC 3 cut(s) 40, 135, 148
Bst2UI CCWGG 1 cut(s) 639
Bst4CI ACNGT 2 cut(s) 408, 432
Bst6I CTCTTC 2 cut(s) 303, 576
BstAFI CTTAAG 1 cut(s) 1007
BstBI TTCGAA 1 cut(s) 727
BstDEI CTNAG 4 cut(s) 132, 413, 530, 656
BstF5I GGATG 4 cut(s) 280, 343, 561, 571
BstFNI CGCG 1 cut(s) 609
BstHHI GCGC 1 cut(s) 609
BstKTI GATC 3 cut(s) 43, 138, 151
BstMBI GATC 3 cut(s) 40, 135, 148
BstMWI GCNNNNNNNGC 1 cut(s) 754
BstNI CCWGG 1 cut(s) 639
BstNSI RCATGY 1 cut(s) 637
BstSCI CCNGG 1 cut(s) 637
BstSFI CTRYAG 3 cut(s) 47, 98, 108
BstSLI GKGCMC 1 cut(s) 750
BstUI CGCG 1 cut(s) 609
BstV2I GAAGAC 2 cut(s) 96, 708
BstX2I RGATCY 1 cut(s) 135
BstYI RGATCY 1 cut(s) 135
Bsu36I CCTNAGG 1 cut(s) 530
BsuRI GGCC 2 cut(s) 357, 454
BtsCI GGATG 4 cut(s) 280, 343, 561, 571
BtsIMutI CAGTG 2 cut(s) 386, 404
BveI ACCTGC 1 cut(s) 40
CaiI CAGNNNCTG 1 cut(s) 388
CfoI GCGC 1 cut(s) 609
Cfr13I GGNCC 2 cut(s) 52, 945
Csp6I GTAC 7 cut(s) 11, 105, 433, 647, 820, 955, 970
CviAII CATG 4 cut(s) 239, 627, 634, 694
CviQI GTAC 7 cut(s) 11, 105, 433, 647, 820, 955, 970
DdeI CTNAG 4 cut(s) 132, 413, 530, 656
DpnI GATC 3 cut(s) 42, 137, 150
DpnII GATC 3 cut(s) 40, 135, 148
EaeI YGGCCR 2 cut(s) 355, 452
Eam1104I CTCTTC 2 cut(s) 303, 576
EarI CTCTTC 2 cut(s) 303, 576
Ecl136II GAGCTC 1 cut(s) 526
Eco24I GRGCYC 1 cut(s) 528
Eco32I GATATC 1 cut(s) 870
Eco47I GGWCC 2 cut(s) 52, 945
Eco53kI GAGCTC 1 cut(s) 526
Eco81I CCTNAGG 1 cut(s) 530
Eco88I CYCGRG 1 cut(s) 163
EcoICRI GAGCTC 1 cut(s) 526
EcoO109I RGGNCCY 2 cut(s) 52, 945
EcoRII CCWGG 1 cut(s) 637
EcoRV GATATC 1 cut(s) 870
EcoT22I ATGCAT 1 cut(s) 880
EcoT38I GRGCYC 1 cut(s) 528
FaeI CATG 4 cut(s) 242, 630, 637, 697
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FaqI GGGAC 2 cut(s) 38, 931
FatI CATG 4 cut(s) 238, 626, 633, 693
FokI GGATG 4 cut(s) 287, 350, 568, 578
FriOI GRGCYC 1 cut(s) 528
FspBI CTAG 1 cut(s) 650
GlaI GCGC 1 cut(s) 608
HaeIII GGCC 2 cut(s) 357, 454
HapII CCGG 2 cut(s) 335, 714
HhaI GCGC 1 cut(s) 609
Hin1II CATG 4 cut(s) 242, 630, 637, 697
Hin6I GCGC 1 cut(s) 607
HinP1I GCGC 1 cut(s) 607
HindIII AAGCTT 1 cut(s) 600
HinfI GANTC 2 cut(s) 5, 471
HpaII CCGG 2 cut(s) 335, 714
HphI GGTGA 2 cut(s) 241, 749
Hpy166II GTNNAC 1 cut(s) 613
Hpy188I TCNGA 3 cut(s) 133, 400, 670
Hpy188III TCNNGA 6 cut(s) 23, 73, 163, 165, 415, 587
Hpy8I GTNNAC 1 cut(s) 613
HpyAV CCTTC 4 cut(s) 265, 728, 955, 991
HpyCH4III ACNGT 2 cut(s) 408, 432
HpyCH4V TGCA 8 cut(s) 49, 110, 262, 374, 404, 630, 697, 878
HpyF10VI GCNNNNNNNGC 1 cut(s) 754
HpyF3I CTNAG 4 cut(s) 132, 413, 530, 656
Hsp92II CATG 4 cut(s) 242, 630, 637, 697
HspAI GCGC 1 cut(s) 607
KflI GGGWCCC 1 cut(s) 945
Kzo9I GATC 3 cut(s) 40, 135, 148
LguI GCTCTTC 1 cut(s) 576
LmnI GCTCC 3 cut(s) 181, 531, 825
LweI GCATC 4 cut(s) 265, 391, 556, 887
MaeI CTAG 1 cut(s) 650
MaeIII GTNAC 3 cut(s) 592, 709, 844
MalI GATC 3 cut(s) 42, 137, 150
MboI GATC 3 cut(s) 40, 135, 148
MflI RGATCY 1 cut(s) 135
MhlI GDGCHC 2 cut(s) 528, 750
MlsI TGGCCA 1 cut(s) 454
MluCI AATT 4 cut(s) 321, 513, 723, 759
MluNI TGGCCA 1 cut(s) 454
MmeI TCCRAC 2 cut(s) 285, 766
Mox20I TGGCCA 1 cut(s) 454
Mph1103I ATGCAT 1 cut(s) 880
MscI TGGCCA 1 cut(s) 454
MseI TTAA 4 cut(s) 507, 576, 780, 1008
MslI CAYNNNNRTG 1 cut(s) 692
Msp20I TGGCCA 1 cut(s) 454
MspCI CTTAAG 1 cut(s) 1007
MspI CCGG 2 cut(s) 335, 714
MspR9I CCNGG 1 cut(s) 639
MvaI CCWGG 1 cut(s) 639
MvnI CGCG 1 cut(s) 609
MwoI GCNNNNNNNGC 1 cut(s) 754
NdeII GATC 3 cut(s) 40, 135, 148
NlaIII CATG 4 cut(s) 242, 630, 637, 697
NlaIV GGNNCC 4 cut(s) 54, 747, 946, 947
NmuCI GTSAC 1 cut(s) 844
NsiI ATGCAT 1 cut(s) 880
NspI RCATGY 1 cut(s) 637
NspV TTCGAA 1 cut(s) 727
PaeR7I CTCGAG 1 cut(s) 163
PciSI GCTCTTC 1 cut(s) 576
PfeI GAWTC 2 cut(s) 5, 471
PpuMI RGGWCCY 2 cut(s) 52, 945
Psp124BI GAGCTC 1 cut(s) 528
Psp5II RGGWCCY 2 cut(s) 52, 945
Psp6I CCWGG 1 cut(s) 637
PspGI CCWGG 1 cut(s) 637
PspN4I GGNNCC 4 cut(s) 54, 747, 946, 947
PspPI GGNCC 2 cut(s) 52, 945
PspPPI RGGWCCY 2 cut(s) 52, 945
PsrI GAACNNNNNNTAC 1 cut(s) 35
PstI CTGCAG 2 cut(s) 51, 112
PstNI CAGNNNCTG 1 cut(s) 388
PsuI RGATCY 1 cut(s) 135
RsaI GTAC 7 cut(s) 12, 106, 434, 648, 821, 956, 971
RsaNI GTAC 7 cut(s) 11, 105, 433, 647, 820, 955, 970
RseI CAYNNNNRTG 1 cut(s) 692
SacI GAGCTC 1 cut(s) 528
SapI GCTCTTC 1 cut(s) 576
SaqAI TTAA 4 cut(s) 507, 576, 780, 1008
Sau3AI GATC 3 cut(s) 40, 135, 148
Sau96I GGNCC 2 cut(s) 52, 945
ScaI AGTACT 2 cut(s) 106, 648
ScrFI CCNGG 1 cut(s) 639
SduI GDGCHC 2 cut(s) 528, 750
SfaNI GCATC 4 cut(s) 265, 391, 556, 887
SfcI CTRYAG 3 cut(s) 47, 98, 108
Sfr274I CTCGAG 1 cut(s) 163
SfuI TTCGAA 1 cut(s) 727
SinI GGWCC 2 cut(s) 52, 945
SlaI CTCGAG 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 2 cut(s) 163, 1007
SmoI CTYRAG 2 cut(s) 163, 1007
Sse9I AATT 4 cut(s) 321, 513, 723, 759
SsiI CCGC 3 cut(s) 368, 501, 662
SspMI CTAG 1 cut(s) 650
SstI GAGCTC 1 cut(s) 528
StyD4I CCNGG 1 cut(s) 637
TaaI ACNGT 2 cut(s) 408, 432
TaqI TCGA 3 cut(s) 164, 727, 863
TasI AATT 4 cut(s) 321, 513, 723, 759
TatI WGTACW 5 cut(s) 104, 432, 646, 819, 954
TfiI GAWTC 2 cut(s) 5, 471
Tru1I TTAA 4 cut(s) 507, 576, 780, 1008
Tru9I TTAA 4 cut(s) 507, 576, 780, 1008
TscAI CASTG 2 cut(s) 393, 411
TseFI GTSAC 1 cut(s) 844
Tsp45I GTSAC 1 cut(s) 844
TspDTI ATGAA 3 cut(s) 284, 572, 874
TspGWI ACGGA 2 cut(s) 291, 987
TspRI CASTG 2 cut(s) 393, 411
Vha464I CTTAAG 1 cut(s) 1007
VpaK11BI GGWCC 2 cut(s) 52, 945
XapI RAATTY 2 cut(s) 321, 723
XceI RCATGY 1 cut(s) 637
XhoI CTCGAG 1 cut(s) 163
XspI CTAG 1 cut(s) 650
ZrmI AGTACT 2 cut(s) 106, 648
Zsp2I ATGCAT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.