MD04G1132100.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000451
Physical Location & Seq
Reverse (-)
21884039 .. 21884776
738 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1132100.v1.1.491

Sequence Viewer

Length: 450 bp
ATGACCTCTCGAGTAAACGTTCAGGAAATTGTTAGGTCTAACCCTTTGCAGGTCCCTGAAAAATTCCTCGTAAGTAGGAAGGAAGAAGACGAGCCAAAGAGTACGGCAGACGTGTTTGATCTGTCTTCGAGGATTCCTATCATTGATCTTTCTCTACTATCGAGAGGGCACAAGGAGGAGCTCAACAAACTGGACCAAGCTTGCAAAGAATGGGGATTCTTTCAGGTTGTGAATCATGGAGTGGCAACAGAAGTGCTGCAGGGGATGAAAGATGCAACGGTCAAGTTCTTTGAACTTCCGTTAGAAGAGAAGAATAAAATTCGTTTTCCACCGGGTGGGATTGACGGCTACAGTCAAATCAAAGTAGTTCCGGAAGGGCAGGCAATTGACTGGTCTGATGGACTGGTTCTCAATATTTATCCAGCCAACGGCAGAAACCTTGAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N Thaumatin

Protein Analysis

150

Amino Acids

16.83

Weight (kDa)

5.11

Isoelectric Point (pI)

51.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 45 - 141 5.2e-28 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000451)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13280 FvH4_6g13281 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13300 FvH4_6g13322
malus_domestica MD04G1132000.v1.1 MD04G1132100.v1.1 MD04G1132400.v1.1 MD12G1145100.v1.1 MD12G1145200.v1.1
prunus_persica Prupe.6G258600_v2.0.a1 Prupe.6G258700_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258900_v2.0.a1
pyrus_communis pycom04g12010 pycom04g12020
rosa_chinensis RchiOBHm_Chr3g0466131 RchiOBHm_Chr3g0466151 RchiOBHm_Chr3g0466161 RchiOBHm_Chr3g0466171 RchiOBHm_Chr3g0466181 RchiOBHm_Chr3g0466201 RchiOBHm_Chr3g0466211 RchiOBHm_Chr6g0251841
rosa_laevigata RLG00000015425 RLG00000024596 RLG00000024599 RLG00000024600 RLG00000024601 RLG00000024602
rosa_multiflora Rmu_co8112718.1_g000001 Rmu_co8178858.1_g000001 Rmu_co8388385.1_g000001 Rmu_sc0000350.1_g000002 Rmu_sc0000350.1_g000003 Rmu_sc0000350.1_g000004 Rmu_sc0000350.1_g000006 Rmu_sc0000350.1_g000011 Rmu_sc0002249.1_g000004 Rmu_sc0007597.1_g000012 Rmu_sc0012177.1_g000002 Rmu_sc0012177.1_g000006 Rmu_sc0012177.1_g000007 Rmu_sc0022084.1_g000003 Rmu_ssc0000303.1_g000004
rosa_roxburghii Rroxscaffold_6G00414720 Rroxscaffold_6G00414730 Rroxscaffold_6G00414740 Rroxscaffold_6G00414750 Rroxscaffold_6G00414760 Rroxscaffold_6G00414770 Rroxscaffold_6G00414780 Rroxscaffold_6G00414790
rosa_rugosa Rorug03G0079600 Rorug03G0079600 Rorug03G0079800 Rorug03G0080100 Rorug03G0080200 Rorug03G0080300 Rorug03G0080400 Rorug05G0504900
rosa_samantha Rh3BG147200 Rh3BG147300 Rh3BG147400 Rh3BG147500 Rh3BG147600 Rh3CG147900 Rh3CG148000 Rh3CG148100 Rh3CG148200 Rh3CG148300 Rh3CG148500 Rh3CG148600 Rh3DG147800 Rh3DG147900 Rh3DG148100 Rh3DG148300 Rh4BG237100 Rh6AG015600 Rh6AG015700 Rh6BG013700 Rh6CG069500
rosa_wichuraiana Rw3G011870 Rw3G011880 Rw3G011890 Rw3G011900 Rw3G011910 Rw3G011930 Rw6G001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 40
AccB7I CCANNNNNTGG 1 cut(s) 335
AccIII TCCGGA 1 cut(s) 370
AclI AACGTT 1 cut(s) 18
AcsI RAATTY 2 cut(s) 62, 318
AdeI CACNNNGTG 1 cut(s) 335
AfaI GTAC 1 cut(s) 103
AfiI CCNNNNNNNGG 3 cut(s) 49, 335, 428
AflIII ACRYGT 1 cut(s) 111
AgsI TTSAA 1 cut(s) 293
AjiI CACGTC 1 cut(s) 112
AluBI AGCT 2 cut(s) 181, 200
AluI AGCT 2 cut(s) 181, 200
Alw21I GWGCWC 1 cut(s) 183
Ama87I CYCGRG 1 cut(s) 9
Aor13HI TCCGGA 1 cut(s) 370
ApeKI GCWGC 1 cut(s) 256
ApoI RAATTY 2 cut(s) 62, 318
AspS9I GGNCC 2 cut(s) 52, 193
AsuC2I CCSGG 1 cut(s) 333
AvaI CYCGRG 1 cut(s) 9
AvaII GGWCC 2 cut(s) 52, 193
BaeGI GKGCMC 1 cut(s) 171
BanII GRGCYC 1 cut(s) 183
BbsI GAAGAC 2 cut(s) 93, 117
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 1 cut(s) 243
BccI CCATC 1 cut(s) 392
BceAI ACGGC 3 cut(s) 120, 361, 445
BcnI CCSGG 1 cut(s) 333
BfmI CTRYAG 2 cut(s) 257, 349
BfuAI ACCTGC 1 cut(s) 40
BisI GCNGC 1 cut(s) 257
BlsI GCNGC 1 cut(s) 258
Bme1390I CCNGG 1 cut(s) 333
Bme18I GGWCC 2 cut(s) 52, 193
BmeT110I CYCGRG 1 cut(s) 9
BmgBI CACGTC 1 cut(s) 112
BmgT120I GGNCC 2 cut(s) 52, 193
BmiI GGNNCC 1 cut(s) 54
BmrFI CCNGG 1 cut(s) 333
BmsI GCATC 1 cut(s) 262
BpiI GAAGAC 2 cut(s) 93, 117
BpuMI CCSGG 1 cut(s) 333
BsaBI GATNNNNATC 1 cut(s) 137
BsaWI WCCGGW 1 cut(s) 370
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 335, 428
Bse1I ACTGG 3 cut(s) 195, 395, 408
Bse8I GATNNNNATC 1 cut(s) 137
BseAI TCCGGA 1 cut(s) 370
BseGI GGATG 1 cut(s) 270
BseJI GATNNNNATC 1 cut(s) 137
BseLI CCNNNNNNNGG 3 cut(s) 49, 335, 428
BseNI ACTGG 3 cut(s) 195, 395, 408
BseRI GAGGAG 1 cut(s) 191
BseSI GKGCMC 1 cut(s) 171
BseXI GCAGC 1 cut(s) 243
BsiHKAI GWGCWC 1 cut(s) 183
BsiHKCI CYCGRG 1 cut(s) 9
BsiSI CCGG 2 cut(s) 332, 371
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 3 cut(s) 49, 335, 428
BsmFI GGGAC 1 cut(s) 38
BsoBI CYCGRG 1 cut(s) 9
Bsp1286I GDGCHC 2 cut(s) 171, 183
Bsp13I TCCGGA 1 cut(s) 370
Bsp143I GATC 2 cut(s) 118, 145
BspEI TCCGGA 1 cut(s) 370
BspLI GGNNCC 1 cut(s) 54
BspMAI CTGCAG 1 cut(s) 261
BspMI ACCTGC 1 cut(s) 40
BsrI ACTGG 3 cut(s) 195, 395, 408
BssMI GATC 2 cut(s) 118, 145
Bst4CI ACNGT 2 cut(s) 280, 353
Bst6I CTCTTC 1 cut(s) 300
BstC8I GCNNGC 2 cut(s) 202, 381
BstF5I GGATG 1 cut(s) 270
BstKTI GATC 2 cut(s) 121, 148
BstMBI GATC 2 cut(s) 118, 145
BstSCI CCNGG 1 cut(s) 331
BstSFI CTRYAG 2 cut(s) 257, 349
BstSLI GKGCMC 1 cut(s) 171
BstV1I GCAGC 1 cut(s) 243
BstV2I GAAGAC 2 cut(s) 93, 117
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 1 cut(s) 270
BveI ACCTGC 1 cut(s) 40
Cac8I GCNNGC 2 cut(s) 202, 381
Cfr13I GGNCC 2 cut(s) 52, 193
Csp6I GTAC 1 cut(s) 102
CviAII CATG 1 cut(s) 236
CviJI RGCY 5 cut(s) 94, 181, 200, 348, 425
CviKI_1 RGCY 5 cut(s) 94, 181, 200, 348, 425
CviQI GTAC 1 cut(s) 102
DpnI GATC 2 cut(s) 120, 147
DpnII GATC 2 cut(s) 118, 145
DraIII CACNNNGTG 1 cut(s) 335
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Ecl136II GAGCTC 1 cut(s) 181
Eco24I GRGCYC 1 cut(s) 183
Eco47I GGWCC 2 cut(s) 52, 193
Eco53kI GAGCTC 1 cut(s) 181
Eco88I CYCGRG 1 cut(s) 9
EcoICRI GAGCTC 1 cut(s) 181
EcoO109I RGGNCCY 1 cut(s) 52
EcoT38I GRGCYC 1 cut(s) 183
FaeI CATG 1 cut(s) 239
FaiI YATR 1 cut(s) 237
FaqI GGGAC 1 cut(s) 38
FatI CATG 1 cut(s) 235
Fnu4HI GCNGC 1 cut(s) 257
FokI GGATG 1 cut(s) 277
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 1 cut(s) 257
GluI GCNGC 1 cut(s) 257
HapII CCGG 2 cut(s) 332, 371
Hin1II CATG 1 cut(s) 239
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 3 cut(s) 133, 216, 232
HpaII CCGG 2 cut(s) 332, 371
Hpy166II GTNNAC 1 cut(s) 16
Hpy188I TCNGA 1 cut(s) 397
Hpy188III TCNNGA 4 cut(s) 9, 23, 162, 371
Hpy8I GTNNAC 1 cut(s) 16
HpyAV CCTTC 2 cut(s) 73, 368
HpyCH4III ACNGT 2 cut(s) 280, 353
HpyCH4IV ACGT 2 cut(s) 18, 111
HpyCH4V TGCA 4 cut(s) 49, 204, 259, 275
HpySE526I ACGT 2 cut(s) 18, 111
Hsp92II CATG 1 cut(s) 239
Kpn2I TCCGGA 1 cut(s) 370
Kzo9I GATC 2 cut(s) 118, 145
LmnI GCTCC 1 cut(s) 178
Lsp1109I GCAGC 1 cut(s) 243
LweI GCATC 1 cut(s) 262
MaeII ACGT 2 cut(s) 18, 111
MalI GATC 2 cut(s) 120, 147
MboI GATC 2 cut(s) 118, 145
MboII GAAGA 5 cut(s) 95, 98, 117, 317, 322
MfeI CAATTG 1 cut(s) 384
MhlI GDGCHC 2 cut(s) 171, 183
MluCI AATT 4 cut(s) 27, 62, 318, 384
MnlI CCTC 5 cut(s) 16, 77, 123, 158, 169
MroI TCCGGA 1 cut(s) 370
MspI CCGG 2 cut(s) 332, 371
MspR9I CCNGG 1 cut(s) 333
MunI CAATTG 1 cut(s) 384
NciI CCSGG 1 cut(s) 333
NdeII GATC 2 cut(s) 118, 145
NlaIII CATG 1 cut(s) 239
NlaIV GGNNCC 1 cut(s) 54
PaeR7I CTCGAG 1 cut(s) 9
PfeI GAWTC 3 cut(s) 133, 216, 232
PflMI CCANNNNNTGG 1 cut(s) 335
PkrI GCNGC 1 cut(s) 258
PpuMI RGGWCCY 1 cut(s) 52
Psp124BI GAGCTC 1 cut(s) 183
Psp1406I AACGTT 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 52
PspN4I GGNNCC 1 cut(s) 54
PspPI GGNCC 2 cut(s) 52, 193
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 1 cut(s) 261
RsaI GTAC 1 cut(s) 103
RsaNI GTAC 1 cut(s) 102
SacI GAGCTC 1 cut(s) 183
SatI GCNGC 1 cut(s) 257
Sau3AI GATC 2 cut(s) 118, 145
Sau96I GGNCC 2 cut(s) 52, 193
ScrFI CCNGG 1 cut(s) 333
SduI GDGCHC 2 cut(s) 171, 183
SetI ASST 9 cut(s) 8, 21, 38, 54, 114, 183, 202, 228, 441
SfaNI GCATC 1 cut(s) 262
SfcI CTRYAG 2 cut(s) 257, 349
Sfr274I CTCGAG 1 cut(s) 9
SinI GGWCC 2 cut(s) 52, 193
SlaI CTCGAG 1 cut(s) 9
SmlI CTYRAG 2 cut(s) 9, 440
SmoI CTYRAG 2 cut(s) 9, 440
Sse9I AATT 4 cut(s) 27, 62, 318, 384
SspI AATATT 1 cut(s) 415
SstI GAGCTC 1 cut(s) 183
StyD4I CCNGG 1 cut(s) 331
TaaI ACNGT 2 cut(s) 280, 353
TaiI ACGT 2 cut(s) 21, 114
TaqI TCGA 3 cut(s) 10, 128, 161
TasI AATT 4 cut(s) 27, 62, 318, 384
TfiI GAWTC 3 cut(s) 133, 216, 232
TseI GCWGC 1 cut(s) 256
TspDTI ATGAA 1 cut(s) 281
TspGWI ACGGA 1 cut(s) 288
Van91I CCANNNNNTGG 1 cut(s) 335
VpaK11BI GGWCC 2 cut(s) 52, 193
XapI RAATTY 2 cut(s) 62, 318
XhoI CTCGAG 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.