RchiOBHm_Chr3g0466201

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000451
Physical Location & Seq
Forward (+)
12709479 .. 12711509
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43230

Sequence Viewer

Length: 1068 bp
ATGGAATCAAACTCAGCCCTTTATGCCCCATGTCCCTCACTACCAAATGTTCAAGAAATGGTGAGGACTGACCCTTTTCAGGTACCTGAAAAATACATCAGGAATGAAACAGACATTACAAAAGATGCAGGTTTGTGTCCTCATCTTTCTTCTGAGGTTCCCATCATTGATCTTTCACTGCTCTCAAAAGGGCACAAAGAGGAGCTCACCAAGCTAGACCAGGCTTGCAAGGAATGGGGTTTCTTTCAGGTGGTAAATCACGGAGTGGCGACAGAAGTAACCAAGAGCTTGAAAGAAGCGGCGGAGAAGTTCTTTGAACTTCCATTAGAAGAGAAGAACAGAATTGCTATGCCATCAAATGACATACAAGGCTATGGCCATACCAATGTGGATCAGAAGGTGCTGGATTGGTCCGACAAACTTGTTCTTGTCGTTTACCCACACCAGTACAGAAAGCTCGATGTTTGGCCGCCCACTCCATTCAAGGAGGCTATTGAGACATACTCTAGTGAAGTTAGAAGGGTTGGAGAGGAGCTTCTCAGGTCTCTATCGTTAATCATGGGGATGGAGAAGGATGCTGTTCTCGGACTACATCAAGAGTTGGTCCAACCTCTGTCTGTAGCCTACTATCCTCAGTGCTCAATGCCTGAGAAAGTACTTGGTCTAAGTGCACACTCAGACAAAGGGAGCTTAGCCATAGTTATGCAAGAAGATGATGTAATGGGACTACAAATCAAACACAACGGAAAATGGGTACCAATCAAGCCCATTCACGATGCCTTCACTGTGAATGTTGGAGATATTATTGAGATATGGAGTAATGGGAAGTACAAGAGCATTGAACATAGAGTTGTAACAAACGAAAGCAGGGCGAGGATCTCTTATTCAACTTTTTGTTTACCACATCAAAATGCAGAAATTGAACCCCTGGATCAAATGATTCTTGCGTCACCGGGGTCTTTTCCGTTGTACAAGAAAGTCACTTATGGGGATTATCTGAGGGAAACCAAGAAGATGAAATCTGAAGGGAAGGCACATGTCGTTGAAGTTGCCAAGATTGTAAGTTGA

Protein Analysis

355

Amino Acids

40.08

Weight (kDa)

5.78

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 53 - 158 3.2e-28 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 205 - 301 9.2e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000451)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13280 FvH4_6g13281 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13300 FvH4_6g13322
malus_domestica MD04G1132000.v1.1 MD04G1132100.v1.1 MD04G1132400.v1.1 MD12G1145100.v1.1 MD12G1145200.v1.1
prunus_persica Prupe.6G258600_v2.0.a1 Prupe.6G258700_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258900_v2.0.a1
pyrus_communis pycom04g12010 pycom04g12020
rosa_chinensis RchiOBHm_Chr3g0466131 RchiOBHm_Chr3g0466151 RchiOBHm_Chr3g0466161 RchiOBHm_Chr3g0466171 RchiOBHm_Chr3g0466181 RchiOBHm_Chr3g0466201 RchiOBHm_Chr3g0466211 RchiOBHm_Chr6g0251841
rosa_laevigata RLG00000015425 RLG00000024596 RLG00000024599 RLG00000024600 RLG00000024601 RLG00000024602
rosa_multiflora Rmu_co8112718.1_g000001 Rmu_co8178858.1_g000001 Rmu_co8388385.1_g000001 Rmu_sc0000350.1_g000002 Rmu_sc0000350.1_g000003 Rmu_sc0000350.1_g000004 Rmu_sc0000350.1_g000006 Rmu_sc0000350.1_g000011 Rmu_sc0002249.1_g000004 Rmu_sc0007597.1_g000012 Rmu_sc0012177.1_g000002 Rmu_sc0012177.1_g000006 Rmu_sc0012177.1_g000007 Rmu_sc0022084.1_g000003 Rmu_ssc0000303.1_g000004
rosa_roxburghii Rroxscaffold_6G00414720 Rroxscaffold_6G00414730 Rroxscaffold_6G00414740 Rroxscaffold_6G00414750 Rroxscaffold_6G00414760 Rroxscaffold_6G00414770 Rroxscaffold_6G00414780 Rroxscaffold_6G00414790
rosa_rugosa Rorug03G0079600 Rorug03G0079600 Rorug03G0079800 Rorug03G0080100 Rorug03G0080200 Rorug03G0080300 Rorug03G0080400 Rorug05G0504900
rosa_samantha Rh3BG147200 Rh3BG147300 Rh3BG147400 Rh3BG147500 Rh3BG147600 Rh3CG147900 Rh3CG148000 Rh3CG148100 Rh3CG148200 Rh3CG148300 Rh3CG148500 Rh3CG148600 Rh3DG147800 Rh3DG147900 Rh3DG148100 Rh3DG148300 Rh4BG237100 Rh6AG015600 Rh6AG015700 Rh6BG013700 Rh6CG069500
rosa_wichuraiana Rw3G011870 Rw3G011880 Rw3G011890 Rw3G011900 Rw3G011910 Rw3G011930 Rw6G001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 119
Acc65I GGTACC 2 cut(s) 82, 754
AccB1I GGYRCC 2 cut(s) 82, 754
AciI CCGC 3 cut(s) 299, 302, 470
AclWI GGATC 3 cut(s) 399, 884, 939
AcoI YGGCCR 2 cut(s) 376, 467
AcuI CTGAAG 1 cut(s) 1044
AdeI CACNNNGTG 1 cut(s) 265
AfaI GTAC 6 cut(s) 84, 449, 657, 756, 830, 971
AfiI CCNNNNNNNGG 1 cut(s) 79
AflIII ACRYGT 1 cut(s) 1036
AgsI TTSAA 8 cut(s) 53, 292, 317, 484, 842, 888, 923, 1046
AjnI CCWGG 2 cut(s) 219, 927
AluBI AGCT 6 cut(s) 205, 214, 288, 457, 535, 690
AluI AGCT 6 cut(s) 205, 214, 288, 457, 535, 690
Alw21I GWGCWC 3 cut(s) 207, 641, 673
Alw26I GTCTC 2 cut(s) 491, 549
Alw44I GTGCAC 1 cut(s) 669
AlwI GGATC 3 cut(s) 399, 884, 939
AoxI GGCC 2 cut(s) 376, 467
ApaLI GTGCAC 1 cut(s) 669
Asp718I GGTACC 2 cut(s) 82, 754
AspS9I GGNCC 2 cut(s) 411, 604
AsuC2I CCSGG 1 cut(s) 954
AsuHPI GGTGA 3 cut(s) 73, 199, 942
AvaII GGWCC 2 cut(s) 411, 604
BaeGI GKGCMC 2 cut(s) 195, 673
BalI TGGCCA 1 cut(s) 378
BanI GGYRCC 2 cut(s) 82, 754
BanII GRGCYC 1 cut(s) 207
Bbv12I GWGCWC 3 cut(s) 207, 641, 673
BccI CCATC 3 cut(s) 170, 361, 559
BciT130I CCWGG 2 cut(s) 221, 929
BcnI CCSGG 1 cut(s) 954
BcoDI GTCTC 2 cut(s) 491, 549
BfaI CTAG 2 cut(s) 215, 507
BfmI CTRYAG 1 cut(s) 618
BfuAI ACCTGC 1 cut(s) 119
BisI GCNGC 2 cut(s) 300, 470
BlpI GCTNAGC 1 cut(s) 691
BlsI GCNGC 2 cut(s) 301, 471
BmcAI AGTACT 1 cut(s) 657
Bme1390I CCNGG 3 cut(s) 221, 929, 954
Bme18I GGWCC 2 cut(s) 411, 604
BmgT120I GGNCC 2 cut(s) 411, 604
BmiI GGNNCC 3 cut(s) 84, 159, 756
BmrFI CCNGG 3 cut(s) 221, 929, 954
BmsI GCATC 3 cut(s) 115, 565, 766
BplI GAGNNNNNCTC 2 cut(s) 488, 520
Bpu1102I GCTNAGC 1 cut(s) 691
BpuMI CCSGG 1 cut(s) 954
BsaI GGTCTC 1 cut(s) 549
BsaJI CCNNGG 2 cut(s) 927, 953
Bsc4I CCNNNNNNNGG 1 cut(s) 79
Bse1I ACTGG 1 cut(s) 445
BseBI CCWGG 2 cut(s) 221, 929
BseDI CCNNGG 2 cut(s) 927, 953
BseGI GGATG 2 cut(s) 570, 580
BseLI CCNNNNNNNGG 1 cut(s) 79
BseMII CTCAG 7 cut(s) 27, 144, 553, 639, 647, 690, 989
BseNI ACTGG 1 cut(s) 445
BseRI GAGGAG 2 cut(s) 215, 545
BseSI GKGCMC 2 cut(s) 195, 673
BshFI GGCC 2 cut(s) 378, 469
BshNI GGYRCC 2 cut(s) 82, 754
BsiHKAI GWGCWC 3 cut(s) 207, 641, 673
BsiSI CCGG 1 cut(s) 953
BslFI GGGAC 2 cut(s) 18, 738
BslI CCNNNNNNNGG 1 cut(s) 79
BsmAI GTCTC 2 cut(s) 491, 549
BsmFI GGGAC 2 cut(s) 18, 738
BsnI GGCC 2 cut(s) 378, 469
Bso31I GGTCTC 1 cut(s) 549
Bsp1286I GDGCHC 4 cut(s) 195, 207, 641, 673
Bsp1407I TGTACA 1 cut(s) 969
Bsp143I GATC 4 cut(s) 169, 391, 876, 931
Bsp1720I GCTNAGC 1 cut(s) 691
BspACI CCGC 3 cut(s) 299, 302, 470
BspANI GGCC 2 cut(s) 378, 469
BspCNI CTCAG 7 cut(s) 26, 145, 552, 640, 646, 689, 990
BspLI GGNNCC 3 cut(s) 84, 159, 756
BspMI ACCTGC 1 cut(s) 119
BspPI GGATC 3 cut(s) 399, 884, 939
BspT107I GGYRCC 2 cut(s) 82, 754
BspTNI GGTCTC 1 cut(s) 549
BsrGI TGTACA 1 cut(s) 969
BsrI ACTGG 1 cut(s) 445
BssECI CCNNGG 2 cut(s) 927, 953
BssMI GATC 4 cut(s) 169, 391, 876, 931
Bst2UI CCWGG 2 cut(s) 221, 929
Bst4CI ACNGT 1 cut(s) 787
Bst6I CTCTTC 1 cut(s) 324
BstAUI TGTACA 1 cut(s) 969
BstC8I GCNNGC 1 cut(s) 226
BstDEI CTNAG 9 cut(s) 13, 153, 539, 633, 648, 665, 676, 691, 998
BstF5I GGATG 2 cut(s) 570, 580
BstKTI GATC 4 cut(s) 172, 394, 879, 934
BstMAI GTCTC 2 cut(s) 491, 549
BstMBI GATC 4 cut(s) 169, 391, 876, 931
BstMWI GCNNNNNNNGC 2 cut(s) 23, 211
BstNI CCWGG 2 cut(s) 221, 929
BstNSI RCATGY 1 cut(s) 1040
BstSCI CCNGG 3 cut(s) 219, 927, 952
BstSFI CTRYAG 1 cut(s) 618
BstSLI GKGCMC 2 cut(s) 195, 673
BstX2I RGATCY 1 cut(s) 876
BstYI RGATCY 1 cut(s) 876
BsuRI GGCC 2 cut(s) 378, 469
BtsCI GGATG 2 cut(s) 570, 580
BtsI GCAGTG 1 cut(s) 176
BtsIMutI CAGTG 3 cut(s) 176, 641, 783
BveI ACCTGC 1 cut(s) 119
Cac8I GCNNGC 1 cut(s) 226
Cfr13I GGNCC 2 cut(s) 411, 604
CseI GACGC 1 cut(s) 936
Csp6I GTAC 6 cut(s) 83, 448, 656, 755, 829, 970
CviAII CATG 3 cut(s) 30, 559, 1037
CviQI GTAC 6 cut(s) 83, 448, 656, 755, 829, 970
DdeI CTNAG 9 cut(s) 13, 153, 539, 633, 648, 665, 676, 691, 998
DpnI GATC 4 cut(s) 171, 393, 878, 933
DpnII GATC 4 cut(s) 169, 391, 876, 931
DraIII CACNNNGTG 1 cut(s) 265
EaeI YGGCCR 2 cut(s) 376, 467
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
EciI GGCGGA 1 cut(s) 317
Ecl136II GAGCTC 1 cut(s) 205
Eco24I GRGCYC 1 cut(s) 207
Eco31I GGTCTC 1 cut(s) 549
Eco47I GGWCC 2 cut(s) 411, 604
Eco53kI GAGCTC 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 1044
EcoICRI GAGCTC 1 cut(s) 205
EcoRII CCWGG 2 cut(s) 219, 927
EcoT38I GRGCYC 1 cut(s) 207
FaeI CATG 3 cut(s) 33, 562, 1040
FaqI GGGAC 2 cut(s) 18, 738
FatI CATG 3 cut(s) 29, 558, 1036
Fnu4HI GCNGC 2 cut(s) 300, 470
FokI GGATG 2 cut(s) 577, 587
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 2 cut(s) 300, 470
FspBI CTAG 2 cut(s) 215, 507
GluI GCNGC 2 cut(s) 300, 470
HaeIII GGCC 2 cut(s) 378, 469
HapII CCGG 1 cut(s) 953
HgaI GACGC 1 cut(s) 936
Hin1II CATG 3 cut(s) 33, 562, 1040
HinfI GANTC 2 cut(s) 5, 940
HpaII CCGG 1 cut(s) 953
HphI GGTGA 3 cut(s) 73, 199, 942
Hpy166II GTNNAC 3 cut(s) 436, 671, 899
Hpy188I TCNGA 7 cut(s) 154, 396, 415, 587, 679, 999, 1024
Hpy188III TCNNGA 4 cut(s) 53, 100, 596, 773
Hpy8I GTNNAC 3 cut(s) 436, 671, 899
HpyAV CCTTC 6 cut(s) 391, 513, 565, 790, 1019, 1024
HpyCH4III ACNGT 1 cut(s) 787
HpyCH4V TGCA 5 cut(s) 128, 228, 671, 706, 914
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 211
HpyF3I CTNAG 9 cut(s) 13, 153, 539, 633, 648, 665, 676, 691, 998
Hsp92II CATG 3 cut(s) 33, 562, 1040
KpnI GGTACC 2 cut(s) 86, 758
Kzo9I GATC 4 cut(s) 169, 391, 876, 931
LmnI GCTCC 3 cut(s) 202, 532, 687
LweI GCATC 3 cut(s) 115, 565, 766
MaeI CTAG 2 cut(s) 215, 507
MaeIII GTNAC 4 cut(s) 277, 853, 948, 979
MalI GATC 4 cut(s) 171, 393, 878, 933
MboI GATC 4 cut(s) 169, 391, 876, 931
MboII GAAGA 5 cut(s) 141, 341, 346, 722, 1024
MflI RGATCY 1 cut(s) 876
MhlI GDGCHC 4 cut(s) 195, 207, 641, 673
MlsI TGGCCA 1 cut(s) 378
MluCI AATT 2 cut(s) 342, 918
MluNI TGGCCA 1 cut(s) 378
MmeI TCCRAC 4 cut(s) 438, 505, 631, 775
Mox20I TGGCCA 1 cut(s) 378
MscI TGGCCA 1 cut(s) 378
MseI TTAA 1 cut(s) 554
MslI CAYNNNNRTG 4 cut(s) 384, 563, 701, 909
Msp20I TGGCCA 1 cut(s) 378
MspI CCGG 1 cut(s) 953
MspR9I CCNGG 3 cut(s) 221, 929, 954
MvaI CCWGG 2 cut(s) 221, 929
MwoI GCNNNNNNNGC 2 cut(s) 23, 211
NciI CCSGG 1 cut(s) 954
NdeII GATC 4 cut(s) 169, 391, 876, 931
NlaIII CATG 3 cut(s) 33, 562, 1040
NlaIV GGNNCC 3 cut(s) 84, 159, 756
NmuCI GTSAC 2 cut(s) 948, 979
NspI RCATGY 1 cut(s) 1040
PciI ACATGT 1 cut(s) 1036
PfeI GAWTC 2 cut(s) 5, 940
PkrI GCNGC 2 cut(s) 301, 471
PscI ACATGT 1 cut(s) 1036
Psp124BI GAGCTC 1 cut(s) 207
Psp6I CCWGG 2 cut(s) 219, 927
PspGI CCWGG 2 cut(s) 219, 927
PspN4I GGNNCC 3 cut(s) 84, 159, 756
PspPI GGNCC 2 cut(s) 411, 604
PsuI RGATCY 1 cut(s) 876
RsaI GTAC 6 cut(s) 84, 449, 657, 756, 830, 971
RsaNI GTAC 6 cut(s) 83, 448, 656, 755, 829, 970
RseI CAYNNNNRTG 4 cut(s) 384, 563, 701, 909
SacI GAGCTC 1 cut(s) 207
SaqAI TTAA 1 cut(s) 554
SatI GCNGC 2 cut(s) 300, 470
Sau3AI GATC 4 cut(s) 169, 391, 876, 931
Sau96I GGNCC 2 cut(s) 411, 604
ScaI AGTACT 1 cut(s) 657
ScrFI CCNGG 3 cut(s) 221, 929, 954
SduI GDGCHC 4 cut(s) 195, 207, 641, 673
SfaNI GCATC 3 cut(s) 115, 565, 766
SfcI CTRYAG 1 cut(s) 618
SinI GGWCC 2 cut(s) 411, 604
SmiMI CAYNNNNRTG 4 cut(s) 384, 563, 701, 909
Sse9I AATT 2 cut(s) 342, 918
SsiI CCGC 3 cut(s) 299, 302, 470
SspMI CTAG 2 cut(s) 215, 507
SstI GAGCTC 1 cut(s) 207
StyD4I CCNGG 3 cut(s) 219, 927, 952
TaaI ACNGT 1 cut(s) 787
TaqI TCGA 1 cut(s) 459
TasI AATT 2 cut(s) 342, 918
TatI WGTACW 4 cut(s) 447, 655, 828, 969
TauI GCSGC 2 cut(s) 302, 472
TfiI GAWTC 2 cut(s) 5, 940
Tru1I TTAA 1 cut(s) 554
Tru9I TTAA 1 cut(s) 554
TscAI CASTG 3 cut(s) 183, 641, 790
TseFI GTSAC 2 cut(s) 948, 979
Tsp45I GTSAC 2 cut(s) 948, 979
TspDTI ATGAA 2 cut(s) 120, 1031
TspGWI ACGGA 3 cut(s) 276, 759, 954
TspRI CASTG 3 cut(s) 183, 641, 790
VneI GTGCAC 1 cut(s) 669
VpaK11BI GGWCC 2 cut(s) 411, 604
XceI RCATGY 1 cut(s) 1040
XspI CTAG 2 cut(s) 215, 507
ZrmI AGTACT 1 cut(s) 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.