RchiOBHm_Chr3g0466171

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000451
Physical Location & Seq
Forward (+)
12695636 .. 12698868
3233 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43227

Sequence Viewer

Length: 873 bp
ATGTTGCCTAGTTTGTGTGTTCGATTTGATTTTCGTTGCTGTACCATTTCTATTGTGATGGTGAATCATGGTGTGGCGACAGAATTGATGAAGGGCATGAAGGATTATGCGCAGAAGTTCTTTGAACTTCCATTAGAAGAAAAGAACAAAATTGCTATACCACCAAATGACATACAAGGCTATGGCCATTCCCATTCGGATCAGATCCTGGATTGGTCCGACAAACTTGTTCTTATCGTTCACCCACACCACTATAGAAAGCTCGAGGTCTGGCCGGCCACTCCATTCAAGGAGGCTATTGAGGCATATTCTAGTGAAGTTAAAAGAGTTGGAGAGGAGCTCTTAAGGTTTATGTCTTTGATCATGGGGATGGAGAAGGATGCTTTTCTTGGGCTACATCAAGAGTTGGTGCAACCCATGTCGGTAGCCTACTATCCTCAATGCTACTTGCCTGATAAAGTACTAGGACTTGGCCCTCACTCAGACAAAGGGACCATAGGCATACTTATGCAAGAAGACGATGTAACGGGACTACAAATCAAGCACGAAGGAAAATGGGTACCGATCAAGCCAATTCCCGACACTTTAGTAGTGAATGTTGGAGACATGATGGAGATATGGAGTAATGGGAAATACAAGAGTATTGAACACAGAGTTGTAACAAACGAAAGCAAGGCGAGGATATCTTGTTCAACGTTTTATTTACCACGTCATGATGTGGAAATTGAACCCCTTGATCAAATGGTACTCGAGTCGCCGGGGTCCCTTCCGATGTACAAAAAGGTCAAATATGGAGATTATCTGAGGCAAACTAAGCATATGAGATTTGAAGGGAAGGCGCATGTCATTAAGGTTGCTAAGCTTAATTGGTAG

Protein Analysis

290

Amino Acids

33.3

Weight (kDa)

6.67

Isoelectric Point (pI)

35.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 19 - 93 1.7e-14 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 140 - 236 1.5e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000451)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13280 FvH4_6g13281 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13300 FvH4_6g13322
malus_domestica MD04G1132000.v1.1 MD04G1132100.v1.1 MD04G1132400.v1.1 MD12G1145100.v1.1 MD12G1145200.v1.1
prunus_persica Prupe.6G258600_v2.0.a1 Prupe.6G258700_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258900_v2.0.a1
pyrus_communis pycom04g12010 pycom04g12020
rosa_chinensis RchiOBHm_Chr3g0466131 RchiOBHm_Chr3g0466151 RchiOBHm_Chr3g0466161 RchiOBHm_Chr3g0466171 RchiOBHm_Chr3g0466181 RchiOBHm_Chr3g0466201 RchiOBHm_Chr3g0466211 RchiOBHm_Chr6g0251841
rosa_laevigata RLG00000015425 RLG00000024596 RLG00000024599 RLG00000024600 RLG00000024601 RLG00000024602
rosa_multiflora Rmu_co8112718.1_g000001 Rmu_co8178858.1_g000001 Rmu_co8388385.1_g000001 Rmu_sc0000350.1_g000002 Rmu_sc0000350.1_g000003 Rmu_sc0000350.1_g000004 Rmu_sc0000350.1_g000006 Rmu_sc0000350.1_g000011 Rmu_sc0002249.1_g000004 Rmu_sc0007597.1_g000012 Rmu_sc0012177.1_g000002 Rmu_sc0012177.1_g000006 Rmu_sc0012177.1_g000007 Rmu_sc0022084.1_g000003 Rmu_ssc0000303.1_g000004
rosa_roxburghii Rroxscaffold_6G00414720 Rroxscaffold_6G00414730 Rroxscaffold_6G00414740 Rroxscaffold_6G00414750 Rroxscaffold_6G00414760 Rroxscaffold_6G00414770 Rroxscaffold_6G00414780 Rroxscaffold_6G00414790
rosa_rugosa Rorug03G0079600 Rorug03G0079600 Rorug03G0079800 Rorug03G0080100 Rorug03G0080200 Rorug03G0080300 Rorug03G0080400 Rorug05G0504900
rosa_samantha Rh3BG147200 Rh3BG147300 Rh3BG147400 Rh3BG147500 Rh3BG147600 Rh3CG147900 Rh3CG148000 Rh3CG148100 Rh3CG148200 Rh3CG148300 Rh3CG148500 Rh3CG148600 Rh3DG147800 Rh3DG147900 Rh3DG148100 Rh3DG148300 Rh4BG237100 Rh6AG015600 Rh6AG015700 Rh6BG013700 Rh6CG069500
rosa_wichuraiana Rw3G011870 Rw3G011880 Rw3G011890 Rw3G011900 Rw3G011910 Rw3G011930 Rw6G001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 111
Acc65I GGTACC 1 cut(s) 559
AccB1I GGYRCC 1 cut(s) 559
AclI AACGTT 1 cut(s) 695
AclWI GGATC 2 cut(s) 199, 207
AcoI YGGCCR 3 cut(s) 184, 272, 276
AfaI GTAC 5 cut(s) 43, 462, 561, 747, 776
AflII CTTAAG 1 cut(s) 343
AgsI TTSAA 6 cut(s) 125, 289, 647, 693, 728, 830
AjiI CACGTC 1 cut(s) 710
AjnI CCWGG 1 cut(s) 207
AloI GAACNNNNNNTCC 2 cut(s) 673, 705
AluBI AGCT 3 cut(s) 262, 340, 862
AluI AGCT 3 cut(s) 262, 340, 862
Alw21I GWGCWC 1 cut(s) 342
Alw26I GTCTC 1 cut(s) 597
AlwI GGATC 2 cut(s) 199, 207
AlwNI CAGNNNCTG 1 cut(s) 208
Ama87I CYCGRG 2 cut(s) 263, 749
AoxI GGCC 4 cut(s) 184, 272, 276, 472
Asp718I GGTACC 1 cut(s) 559
AspLEI GCGC 2 cut(s) 112, 841
AspS9I GGNCC 4 cut(s) 216, 473, 492, 762
AsuC2I CCSGG 1 cut(s) 759
AsuHPI GGTGA 2 cut(s) 73, 233
AvaI CYCGRG 2 cut(s) 263, 749
AvaII GGWCC 3 cut(s) 216, 492, 762
BaeI ACNNNNGTAYC 2 cut(s) 737, 770
BalI TGGCCA 1 cut(s) 186
BanI GGYRCC 1 cut(s) 559
BanII GRGCYC 1 cut(s) 342
BbsI GAAGAC 1 cut(s) 522
Bbv12I GWGCWC 1 cut(s) 342
BccI CCATC 3 cut(s) 52, 364, 604
BciT130I CCWGG 1 cut(s) 209
BclI TGATCA 2 cut(s) 360, 736
BcnI CCSGG 1 cut(s) 759
BcoDI GTCTC 1 cut(s) 597
BfaI CTAG 3 cut(s) 9, 312, 464
BfmI CTRYAG 1 cut(s) 253
BfrI CTTAAG 1 cut(s) 343
BlpI GCTNAGC 1 cut(s) 858
BmcAI AGTACT 1 cut(s) 462
Bme1390I CCNGG 2 cut(s) 209, 759
Bme18I GGWCC 3 cut(s) 216, 492, 762
BmeT110I CYCGRG 2 cut(s) 263, 749
BmgBI CACGTC 1 cut(s) 710
BmgT120I GGNCC 4 cut(s) 216, 473, 492, 762
BmiI GGNNCC 4 cut(s) 493, 561, 763, 764
BmrFI CCNGG 2 cut(s) 209, 759
BmsI GCATC 1 cut(s) 370
BpiI GAAGAC 1 cut(s) 522
BplI GAGNNNNNCTC 2 cut(s) 324, 356
Bpu1102I GCTNAGC 1 cut(s) 858
BpuMI CCSGG 1 cut(s) 759
BsaJI CCNNGG 1 cut(s) 758
Bse118I RCCGGY 1 cut(s) 274
BseBI CCWGG 1 cut(s) 209
BseDI CCNNGG 1 cut(s) 758
BseGI GGATG 2 cut(s) 375, 385
BseMII CTCAG 2 cut(s) 495, 794
BseRI GAGGAG 1 cut(s) 350
BshFI GGCC 4 cut(s) 186, 274, 278, 474
BshNI GGYRCC 1 cut(s) 559
BsiHKAI GWGCWC 1 cut(s) 342
BsiHKCI CYCGRG 2 cut(s) 263, 749
BsiSI CCGG 2 cut(s) 275, 758
BslFI GGGAC 3 cut(s) 505, 543, 748
BsmAI GTCTC 1 cut(s) 597
BsmFI GGGAC 3 cut(s) 505, 543, 748
BsnI GGCC 4 cut(s) 186, 274, 278, 474
BsoBI CYCGRG 2 cut(s) 263, 749
Bsp1286I GDGCHC 1 cut(s) 342
Bsp1407I TGTACA 1 cut(s) 774
Bsp143I GATC 5 cut(s) 199, 204, 360, 564, 736
Bsp1720I GCTNAGC 1 cut(s) 858
BspANI GGCC 4 cut(s) 186, 274, 278, 474
BspCNI CTCAG 2 cut(s) 494, 795
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 4 cut(s) 493, 561, 763, 764
BspPI GGATC 2 cut(s) 199, 207
BspT107I GGYRCC 1 cut(s) 559
BspTI CTTAAG 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 274
BsrGI TGTACA 1 cut(s) 774
BssAI RCCGGY 1 cut(s) 274
BssECI CCNNGG 1 cut(s) 758
BssMI GATC 5 cut(s) 199, 204, 360, 564, 736
Bst2UI CCWGG 1 cut(s) 209
BstAFI CTTAAG 1 cut(s) 343
BstAUI TGTACA 1 cut(s) 774
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 4 cut(s) 481, 803, 813, 858
BstF5I GGATG 2 cut(s) 375, 385
BstHHI GCGC 2 cut(s) 112, 841
BstKTI GATC 5 cut(s) 202, 207, 363, 567, 739
BstMAI GTCTC 1 cut(s) 597
BstMBI GATC 5 cut(s) 199, 204, 360, 564, 736
BstMWI GCNNNNNNNGC 2 cut(s) 302, 814
BstNI CCWGG 1 cut(s) 209
BstNSI RCATGY 1 cut(s) 845
BstSCI CCNGG 2 cut(s) 207, 757
BstSFI CTRYAG 1 cut(s) 253
BstV2I GAAGAC 1 cut(s) 522
BstX2I RGATCY 1 cut(s) 204
BstYI RGATCY 1 cut(s) 204
BsuRI GGCC 4 cut(s) 186, 274, 278, 474
BtrI CACGTC 1 cut(s) 710
BtsCI GGATG 2 cut(s) 375, 385
Cac8I GCNNGC 1 cut(s) 276
CaiI CAGNNNCTG 1 cut(s) 208
CciI TCATGA 1 cut(s) 712
CfoI GCGC 2 cut(s) 112, 841
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 4 cut(s) 216, 473, 492, 762
Csp6I GTAC 5 cut(s) 42, 461, 560, 746, 775
CviAII CATG 7 cut(s) 68, 97, 364, 418, 607, 713, 842
CviQI GTAC 5 cut(s) 42, 461, 560, 746, 775
DdeI CTNAG 4 cut(s) 481, 803, 813, 858
DpnI GATC 5 cut(s) 201, 206, 362, 566, 738
DpnII GATC 5 cut(s) 199, 204, 360, 564, 736
EaeI YGGCCR 3 cut(s) 184, 272, 276
Ecl136II GAGCTC 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 342
Eco32I GATATC 1 cut(s) 684
Eco47I GGWCC 3 cut(s) 216, 492, 762
Eco53kI GAGCTC 1 cut(s) 340
Eco88I CYCGRG 2 cut(s) 263, 749
EcoICRI GAGCTC 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 762
EcoRII CCWGG 1 cut(s) 207
EcoRV GATATC 1 cut(s) 684
EcoT38I GRGCYC 1 cut(s) 342
FaeI CATG 7 cut(s) 71, 100, 367, 421, 610, 716, 845
FaqI GGGAC 3 cut(s) 505, 543, 748
FatI CATG 7 cut(s) 67, 96, 363, 417, 606, 712, 841
FauNDI CATATG 1 cut(s) 819
FbaI TGATCA 2 cut(s) 360, 736
FokI GGATG 2 cut(s) 382, 392
FriOI GRGCYC 1 cut(s) 342
FseI GGCCGGCC 1 cut(s) 278
FspBI CTAG 3 cut(s) 9, 312, 464
FspI TGCGCA 1 cut(s) 111
GlaI GCGC 2 cut(s) 111, 840
HaeIII GGCC 4 cut(s) 186, 274, 278, 474
HapII CCGG 2 cut(s) 275, 758
HhaI GCGC 2 cut(s) 112, 841
Hin1II CATG 7 cut(s) 71, 100, 367, 421, 610, 716, 845
Hin6I GCGC 2 cut(s) 110, 839
HinP1I GCGC 2 cut(s) 110, 839
HindIII AAGCTT 1 cut(s) 860
HinfI GANTC 2 cut(s) 64, 752
HpaII CCGG 2 cut(s) 275, 758
HphI GGTGA 2 cut(s) 73, 233
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 6 cut(s) 199, 204, 220, 484, 771, 804
Hpy188III TCNNGA 3 cut(s) 401, 578, 713
Hpy8I GTNNAC 1 cut(s) 241
HpyAV CCTTC 7 cut(s) 85, 94, 370, 542, 776, 824, 829
HpyCH4IV ACGT 2 cut(s) 695, 709
HpyCH4V TGCA 2 cut(s) 412, 511
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 814
HpyF3I CTNAG 4 cut(s) 481, 803, 813, 858
HpySE526I ACGT 2 cut(s) 695, 709
Hsp92II CATG 7 cut(s) 71, 100, 367, 421, 610, 716, 845
HspAI GCGC 2 cut(s) 110, 839
KflI GGGWCCC 1 cut(s) 762
KpnI GGTACC 1 cut(s) 563
KroI GCCGGC 1 cut(s) 274
KroNI GCCGGC 1 cut(s) 276
Ksp22I TGATCA 2 cut(s) 360, 736
Kzo9I GATC 5 cut(s) 199, 204, 360, 564, 736
LmnI GCTCC 1 cut(s) 337
LpnPI CCDG 6 cut(s) 194, 221, 256, 288, 465, 771
LweI GCATC 1 cut(s) 370
MaeI CTAG 3 cut(s) 9, 312, 464
MaeII ACGT 2 cut(s) 695, 709
MaeIII GTNAC 2 cut(s) 523, 658
MalI GATC 5 cut(s) 201, 206, 362, 566, 738
MboI GATC 5 cut(s) 199, 204, 360, 564, 736
MboII GAAGA 2 cut(s) 149, 527
MflI RGATCY 1 cut(s) 204
MhlI GDGCHC 1 cut(s) 342
MlsI TGGCCA 1 cut(s) 186
MluCI AATT 5 cut(s) 83, 150, 573, 723, 865
MluNI TGGCCA 1 cut(s) 186
MlyI GAGTC 1 cut(s) 761
MmeI TCCRAC 3 cut(s) 243, 310, 580
MnlI CCTC 8 cut(s) 259, 286, 295, 328, 447, 486, 672, 798
Mox20I TGGCCA 1 cut(s) 186
MroNI GCCGGC 1 cut(s) 274
MscI TGGCCA 1 cut(s) 186
MseI TTAA 4 cut(s) 321, 344, 849, 864
MslI CAYNNNNRTG 2 cut(s) 368, 506
Msp20I TGGCCA 1 cut(s) 186
MspCI CTTAAG 1 cut(s) 343
MspI CCGG 2 cut(s) 275, 758
MspR9I CCNGG 2 cut(s) 209, 759
MvaI CCWGG 1 cut(s) 209
MwoI GCNNNNNNNGC 2 cut(s) 302, 814
NaeI GCCGGC 1 cut(s) 276
NciI CCSGG 1 cut(s) 759
NdeI CATATG 1 cut(s) 819
NdeII GATC 5 cut(s) 199, 204, 360, 564, 736
NgoMIV GCCGGC 1 cut(s) 274
NlaIII CATG 7 cut(s) 71, 100, 367, 421, 610, 716, 845
NlaIV GGNNCC 4 cut(s) 493, 561, 763, 764
NsbI TGCGCA 1 cut(s) 111
NspI RCATGY 1 cut(s) 845
PaeR7I CTCGAG 2 cut(s) 263, 749
PagI TCATGA 1 cut(s) 712
PdiI GCCGGC 1 cut(s) 276
PfeI GAWTC 1 cut(s) 64
PfoI TCCNGGA 1 cut(s) 207
PleI GAGTC 1 cut(s) 760
PpsI GAGTC 1 cut(s) 760
PpuMI RGGWCCY 1 cut(s) 762
Psp124BI GAGCTC 1 cut(s) 342
Psp1406I AACGTT 1 cut(s) 695
Psp5II RGGWCCY 1 cut(s) 762
Psp6I CCWGG 1 cut(s) 207
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 4 cut(s) 493, 561, 763, 764
PspPI GGNCC 4 cut(s) 216, 473, 492, 762
PspPPI RGGWCCY 1 cut(s) 762
PspXI VCTCGAGB 2 cut(s) 263, 749
PstNI CAGNNNCTG 1 cut(s) 208
PsuI RGATCY 1 cut(s) 204
RigI GGCCGGCC 1 cut(s) 278
RsaI GTAC 5 cut(s) 43, 462, 561, 747, 776
RsaNI GTAC 5 cut(s) 42, 461, 560, 746, 775
RseI CAYNNNNRTG 2 cut(s) 368, 506
SacI GAGCTC 1 cut(s) 342
SaqAI TTAA 4 cut(s) 321, 344, 849, 864
Sau3AI GATC 5 cut(s) 199, 204, 360, 564, 736
Sau96I GGNCC 4 cut(s) 216, 473, 492, 762
ScaI AGTACT 1 cut(s) 462
SchI GAGTC 1 cut(s) 761
ScrFI CCNGG 2 cut(s) 209, 759
SduI GDGCHC 1 cut(s) 342
SetI ASST 9 cut(s) 264, 270, 342, 350, 698, 712, 786, 855, 864
SfaNI GCATC 1 cut(s) 370
SfcI CTRYAG 1 cut(s) 253
Sfr274I CTCGAG 2 cut(s) 263, 749
SinI GGWCC 3 cut(s) 216, 492, 762
SlaI CTCGAG 2 cut(s) 263, 749
SmiMI CAYNNNNRTG 2 cut(s) 368, 506
SmlI CTYRAG 3 cut(s) 263, 343, 749
SmoI CTYRAG 3 cut(s) 263, 343, 749
Sse9I AATT 5 cut(s) 83, 150, 573, 723, 865
SspMI CTAG 3 cut(s) 9, 312, 464
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 2 cut(s) 207, 757
TaiI ACGT 2 cut(s) 698, 712
TaqI TCGA 3 cut(s) 22, 264, 750
TasI AATT 5 cut(s) 83, 150, 573, 723, 865
TatI WGTACW 2 cut(s) 460, 774
TfiI GAWTC 1 cut(s) 64
Tru1I TTAA 4 cut(s) 321, 344, 849, 864
Tru9I TTAA 4 cut(s) 321, 344, 849, 864
TspDTI ATGAA 2 cut(s) 104, 113
Vha464I CTTAAG 1 cut(s) 343
VpaK11BI GGWCC 3 cut(s) 216, 492, 762
XceI RCATGY 1 cut(s) 845
XhoI CTCGAG 2 cut(s) 263, 749
XspI CTAG 3 cut(s) 9, 312, 464
ZrmI AGTACT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.