MD12G1145200.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000451
Physical Location & Seq
Reverse (-)
22437944 .. 22440193
2250 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1145200.v1.1.491

Sequence Viewer

Length: 387 bp
ATGCCGAAGAGCGCGGATCATATGTGTCAGCTTTCTTCGGAGATTCCTACCACTGATTTCTCTCTGCTTTCAAGTGGGAACACGGAGGAGCTTACCAAACTACGCTTGGCTTGCAAAGAATGGGGCTTCTTCCAGATATGGAGTAATGGGAAGTACAAAAGTATTGAACACAGAGCTGCCACAACTGAAGGCCAGGCAAGGTTATCTTATGCAACATTTCTTATACCACATGATGATGTCGAAATTGAACCATTCGATCACATGATGGACTGTCCACGATCTGTTTGGAAGTACAAGAAAGTCAGATGTGGAGATGATCTGAGGGAATCCTTGAAGATGAAAATGAATGGGAAGGCACACACTCAAGTGGCAAAGATTGAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N Thaumatin

Protein Analysis

129

Amino Acids

14.7

Weight (kDa)

6.95

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 44 - 75 2.2e-06 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000451)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g13280 FvH4_6g13281 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13290 FvH4_6g13300 FvH4_6g13322
malus_domestica MD04G1132000.v1.1 MD04G1132100.v1.1 MD04G1132400.v1.1 MD12G1145100.v1.1 MD12G1145200.v1.1
prunus_persica Prupe.6G258600_v2.0.a1 Prupe.6G258700_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258800_v2.0.a1 Prupe.6G258900_v2.0.a1
pyrus_communis pycom04g12010 pycom04g12020
rosa_chinensis RchiOBHm_Chr3g0466131 RchiOBHm_Chr3g0466151 RchiOBHm_Chr3g0466161 RchiOBHm_Chr3g0466171 RchiOBHm_Chr3g0466181 RchiOBHm_Chr3g0466201 RchiOBHm_Chr3g0466211 RchiOBHm_Chr6g0251841
rosa_laevigata RLG00000015425 RLG00000024596 RLG00000024599 RLG00000024600 RLG00000024601 RLG00000024602
rosa_multiflora Rmu_co8112718.1_g000001 Rmu_co8178858.1_g000001 Rmu_co8388385.1_g000001 Rmu_sc0000350.1_g000002 Rmu_sc0000350.1_g000003 Rmu_sc0000350.1_g000004 Rmu_sc0000350.1_g000006 Rmu_sc0000350.1_g000011 Rmu_sc0002249.1_g000004 Rmu_sc0007597.1_g000012 Rmu_sc0012177.1_g000002 Rmu_sc0012177.1_g000006 Rmu_sc0012177.1_g000007 Rmu_sc0022084.1_g000003 Rmu_ssc0000303.1_g000004
rosa_roxburghii Rroxscaffold_6G00414720 Rroxscaffold_6G00414730 Rroxscaffold_6G00414740 Rroxscaffold_6G00414750 Rroxscaffold_6G00414760 Rroxscaffold_6G00414770 Rroxscaffold_6G00414780 Rroxscaffold_6G00414790
rosa_rugosa Rorug03G0079600 Rorug03G0079600 Rorug03G0079800 Rorug03G0080100 Rorug03G0080200 Rorug03G0080300 Rorug03G0080400 Rorug05G0504900
rosa_samantha Rh3BG147200 Rh3BG147300 Rh3BG147400 Rh3BG147500 Rh3BG147600 Rh3CG147900 Rh3CG148000 Rh3CG148100 Rh3CG148200 Rh3CG148300 Rh3CG148500 Rh3CG148600 Rh3DG147800 Rh3DG147900 Rh3DG148100 Rh3DG148300 Rh4BG237100 Rh6AG015600 Rh6AG015700 Rh6BG013700 Rh6CG069500
rosa_wichuraiana Rw3G011870 Rw3G011880 Rw3G011890 Rw3G011900 Rw3G011910 Rw3G011930 Rw6G001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 14
AciI CCGC 1 cut(s) 14
AclWI GGATC 1 cut(s) 24
AcuI CTGAAG 1 cut(s) 207
AfaI GTAC 2 cut(s) 155, 293
AgsI TTSAA 5 cut(s) 72, 167, 248, 334, 380
AjnI CCWGG 1 cut(s) 192
AleI CACNNNNGTG 1 cut(s) 365
AluBI AGCT 3 cut(s) 31, 91, 176
AluI AGCT 3 cut(s) 31, 91, 176
AlwI GGATC 1 cut(s) 24
AoxI GGCC 1 cut(s) 190
ApeKI GCWGC 1 cut(s) 176
AspLEI GCGC 1 cut(s) 14
BbvI GCAGC 1 cut(s) 163
BccI CCATC 1 cut(s) 259
BciT130I CCWGG 1 cut(s) 194
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
Bme1390I CCNGG 1 cut(s) 194
BmrFI CCNGG 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 348
BseBI CCWGG 1 cut(s) 194
BseMII CTCAG 1 cut(s) 311
BseRI GAGGAG 1 cut(s) 101
BseXI GCAGC 1 cut(s) 163
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 1 cut(s) 192
BsnI GGCC 1 cut(s) 192
Bsp143I GATC 4 cut(s) 16, 256, 278, 316
BspACI CCGC 1 cut(s) 14
BspANI GGCC 1 cut(s) 192
BspCNI CTCAG 1 cut(s) 312
BspFNI CGCG 1 cut(s) 14
BspPI GGATC 1 cut(s) 24
BspQI GCTCTTC 1 cut(s) 2
BssMI GATC 4 cut(s) 16, 256, 278, 316
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 1 cut(s) 272
Bst6I CTCTTC 1 cut(s) 2
BstC8I GCNNGC 1 cut(s) 112
BstDEI CTNAG 1 cut(s) 320
BstFNI CGCG 1 cut(s) 14
BstHHI GCGC 1 cut(s) 14
BstKTI GATC 4 cut(s) 19, 259, 281, 319
BstMBI GATC 4 cut(s) 16, 256, 278, 316
BstMWI GCNNNNNNNGC 1 cut(s) 111
BstNI CCWGG 1 cut(s) 194
BstSCI CCNGG 1 cut(s) 192
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 1 cut(s) 163
BsuRI GGCC 1 cut(s) 192
BtsIMutI CAGTG 1 cut(s) 51
Cac8I GCNNGC 1 cut(s) 112
CfoI GCGC 1 cut(s) 14
Csp6I GTAC 2 cut(s) 154, 292
CviAII CATG 2 cut(s) 230, 262
CviJI RGCY 6 cut(s) 31, 91, 110, 126, 176, 192
CviKI_1 RGCY 6 cut(s) 31, 91, 110, 126, 176, 192
CviQI GTAC 2 cut(s) 154, 292
DdeI CTNAG 1 cut(s) 320
DpnI GATC 4 cut(s) 18, 258, 280, 318
DpnII GATC 4 cut(s) 16, 256, 278, 316
Eam1104I CTCTTC 1 cut(s) 2
EarI CTCTTC 1 cut(s) 2
Eco57I CTGAAG 1 cut(s) 207
EcoRII CCWGG 1 cut(s) 192
FaeI CATG 2 cut(s) 233, 265
FaiI YATR 7 cut(s) 21, 23, 139, 210, 224, 231, 263
FalI AAGNNNNNCTT 2 cut(s) 190, 222
FatI CATG 2 cut(s) 229, 261
FauNDI CATATG 1 cut(s) 21
Fnu4HI GCNGC 1 cut(s) 177
Fsp4HI GCNGC 1 cut(s) 177
GlaI GCGC 1 cut(s) 13
GluI GCNGC 1 cut(s) 177
HaeIII GGCC 1 cut(s) 192
HhaI GCGC 1 cut(s) 14
Hin1II CATG 2 cut(s) 233, 265
Hin6I GCGC 1 cut(s) 12
HinP1I GCGC 1 cut(s) 12
HinfI GANTC 2 cut(s) 43, 326
Hpy166II GTNNAC 1 cut(s) 275
Hpy188I TCNGA 3 cut(s) 40, 305, 321
Hpy188III TCNNGA 1 cut(s) 133
Hpy8I GTNNAC 1 cut(s) 275
HpyAV CCTTC 2 cut(s) 182, 346
HpyCH4III ACNGT 1 cut(s) 272
HpyCH4V TGCA 2 cut(s) 114, 212
HpyF10VI GCNNNNNNNGC 1 cut(s) 111
HpyF3I CTNAG 1 cut(s) 320
Hsp92II CATG 2 cut(s) 233, 265
HspAI GCGC 1 cut(s) 12
Kzo9I GATC 4 cut(s) 16, 256, 278, 316
LguI GCTCTTC 1 cut(s) 2
LmnI GCTCC 1 cut(s) 88
LpnPI CCDG 3 cut(s) 146, 179, 206
Lsp1109I GCAGC 1 cut(s) 163
MalI GATC 4 cut(s) 18, 258, 280, 318
MboI GATC 4 cut(s) 16, 256, 278, 316
MboII GAAGA 4 cut(s) 19, 27, 121, 346
MluCI AATT 1 cut(s) 243
MnlI CCTC 2 cut(s) 79, 315
MseI TTAA 1 cut(s) 385
MslI CAYNNNNRTG 2 cut(s) 234, 365
MspR9I CCNGG 1 cut(s) 194
MvaI CCWGG 1 cut(s) 194
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 1 cut(s) 111
NdeI CATATG 1 cut(s) 21
NdeII GATC 4 cut(s) 16, 256, 278, 316
NlaIII CATG 2 cut(s) 233, 265
OliI CACNNNNGTG 1 cut(s) 365
PciSI GCTCTTC 1 cut(s) 2
PfeI GAWTC 2 cut(s) 43, 326
PkrI GCNGC 1 cut(s) 178
Psp6I CCWGG 1 cut(s) 192
PspGI CCWGG 1 cut(s) 192
RsaI GTAC 2 cut(s) 155, 293
RsaNI GTAC 2 cut(s) 154, 292
RseI CAYNNNNRTG 2 cut(s) 234, 365
SapI GCTCTTC 1 cut(s) 2
SaqAI TTAA 1 cut(s) 385
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 4 cut(s) 16, 256, 278, 316
ScrFI CCNGG 1 cut(s) 194
SetI ASST 4 cut(s) 33, 93, 178, 203
SmiMI CAYNNNNRTG 2 cut(s) 234, 365
SmlI CTYRAG 1 cut(s) 363
SmoI CTYRAG 1 cut(s) 363
Sse9I AATT 1 cut(s) 243
SsiI CCGC 1 cut(s) 14
StyD4I CCNGG 1 cut(s) 192
TaaI ACNGT 1 cut(s) 272
TaqI TCGA 2 cut(s) 240, 255
TasI AATT 1 cut(s) 243
TatI WGTACW 2 cut(s) 153, 291
TfiI GAWTC 2 cut(s) 43, 326
Tru1I TTAA 1 cut(s) 385
Tru9I TTAA 1 cut(s) 385
TscAI CASTG 1 cut(s) 58
TseI GCWGC 1 cut(s) 176
TspDTI ATGAA 2 cut(s) 353, 359
TspGWI ACGGA 1 cut(s) 98
TspRI CASTG 1 cut(s) 58
XcmI CCANNNNNNNNNTGG 2 cut(s) 103, 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.