MD13G1235400.v1.1

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000237
Physical Location & Seq
Forward (+)
23709135 .. 23710360
1226 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1235400.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGGAGAGTCTTTGTCCCGAAGCAGCTGAAACCATAGCCAGCAACGAAGACCTCCTTGCACATATCCTTCTTTGCATGCCCCCTAAATCTCTGCTCCGCTTCAAATCCGTCTCCAAGCACTGGCTCGCTCTTATCTCCGGCCCCAACTTCTGCAACCGCCATGCCCTCCAAAACCCAAATTCCAAGGTCTCTGCCTTCTTCGCCCGGACCATAATATCTCAAGAAATCTTCTTCACCTCTCTCGGCAACGACGAAAATCCATCTGGGTTTCGCTCCAAACCTCTCAATTTTGTCGGCGACCCACGGGGCGTCGAGATTCTGCAGTCCTGCAACGGCTTGCTCCTGTGCTGCTCCTCCCTCAGCACGACCGGAAGGTACTACGTTGTCAATCCCACAACCAGTAAGTTCTCAACGTTCTTTTTTCCAGCTGCTACTAAATCGACAACCGTTTCGGGTGTCGCTTTGGCTTTCGATCCTGCGAAATCGATTCATTACAAGGTTGTCGTCGTTAGTACCATTTCTGAGTCAGTTGGAGGTTACCAATTTAAGGTCTTTTTGTCTGAGACCCGAAGTTGGAAGGTTTTTCGGGCTAATTTCCCTGCAAAATTCGATATGAACTTCAGCCGTGGAGTGCACTGCAATGGACTAATTAGTTGGATTGGCGATGCAAGTAAGGTTTTGCTCTATGACACAGATGAACATCGTTTCAGGACTGTGCCAAGTCCGCCGGGTCACGGCGGCTACCGATGGCATAGATATTTTGGGGAGTCTTGTGGCCATTTGCATCTTATCGAAATTTCTAGGCCGCAACTTTCGCAATTTGAGGTGCTGGAGATGGAGAAGGACTACTCTGGTTGGTTAGTCAAGTACAATGTCGACCTTAATGCCATAAGCTCTGCATTTCCGGAGATGGTTTGCAACCCTCTTGAACCCTACTACTCATTTGTTGTTGTATTTGTTGATGAGCATGATTTGTCTATTTTGCTGCACATTCCCTGTAAGGTCATCTCTTATAATCTTAGGGATAAGAAAGTTTGTGACTTAACTCCCAAAAACATTTACACCAAGAGGTCTCTAATAGTTCGATGGCAATCTGCTTATGCACATATGGACACTTTGGCTTGTAATCAAGAATGTTTTGTATTACCAAATTCTGTGTACTTATGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

391

Amino Acids

43.94

Weight (kDa)

8.14

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 16 - 51 7.4e-08 F-box domain
FBA_1 PF07734 103 - 243 8.9e-12 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000237)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14315
fragaria_vesca FvH4_2g35920 FvH4_2g35930 FvH4_2g35940 FvH4_2g35950 FvH4_3g00321 FvH4_3g32490 FvH4_3g43790 FvH4_3g43790 FvH4_3g43790 FvH4_4g06360 FvH4_4g06370 FvH4_4g06370 FvH4_6g52062 FvH4_6g52062 FvH4_6g52080 FvH4_6g52610 FvH4_6g52830 FvH4_6g52861 FvH4_6g52861 FvH4_6g52862 FvH4_6g52871 FvH4_6g52880 FvH4_6g52931 FvH4_6g52941 FvH4_6g52942 FvH4_6g53001 FvH4_6g53021 FvH4_6g53072 FvH4_6g53610 FvH4_6g53620 FvH4_6g53620 FvH4_6g53651 FvH4_6g53660
malus_domestica MD13G1235400.v1.1 MD16G1240000.v1.1
prunus_persica Prupe.1G066400_v2.0.a1
pyrus_communis pycom13g20790 pycom16g20090
rosa_chinensis RchiOBHm_Chr2g0174451 RchiOBHm_Chr2g0174531 RchiOBHm_Chr2g0174701 RchiOBHm_Chr2g0174881 RchiOBHm_Chr2g0174951 RchiOBHm_Chr4g0396041 RchiOBHm_Chr4g0396501 RchiOBHm_Chr4g0398111 RchiOBHm_Chr5g0059531 RchiOBHm_Chr6g0264241
rosa_laevigata RLG00000009385 RLG00000009528 RLG00000009532 RLG00000009537 RLG00000009541 RLG00000009577 RLG00000022267 RLG00000022268 RLG00000022269 RLG00000022273 RLG00000022287 RLG00000022289 RLG00000032767 RLG00000035297
rosa_multiflora Rmu_co8114622.1_g000001 Rmu_co8160300.1_g000001 Rmu_co8411703.1_g000001 Rmu_sc0001217.1_g000015 Rmu_sc0001533.1_g000002 Rmu_sc0003649.1_g000012 Rmu_sc0003649.1_g000015 Rmu_sc0003649.1_g000017 Rmu_sc0003649.1_g000020 Rmu_sc0006325.1_g000006 Rmu_sc0009506.1_g000001 Rmu_sc0009766.1_g000006 Rmu_sc0015078.1_g000002 Rmu_sc0015594.1_g000006 Rmu_sc0015594.1_g000007
rosa_roxburghii Rroxscaffold_1G00021050 Rroxscaffold_2G00077800 Rroxscaffold_2G00077920 Rroxscaffold_2G00078070 Rroxscaffold_2G00078100 Rroxscaffold_2G00078130 Rroxscaffold_2G00078170 Rroxscaffold_3G00220050 Rroxscaffold_5G00340840 Rroxscaffold_5G00340850 Rroxscaffold_5G00341370 Rroxscaffold_5G00343010 Rroxscaffold_5G00381750
rosa_rugosa Rorug02G0577300 Rorug02G0577400 Rorug02G0577700 Rorug02G0577800 Rorug02G0579000 Rorug03G0352900 Rorug03G0352900 Rorug04G0006000 Rorug05G0323000
rosa_samantha Rh2AG658100 Rh2AG658400 Rh2AG658600 Rh2AG660200 Rh2AG663100 Rh2CG632100 Rh2CG632600 Rh2CG632700 Rh2CG634300 Rh2CG635600 Rh2DG682800 Rh2DG683300 Rh2DG686000 Rh3BG068500 Rh3CG066900 Rh4AG064800 Rh4AG065900 Rh4AG068900 Rh4AG083000 Rh4BG063600 Rh4BG063700 Rh4BG067000 Rh4BG080700 Rh4DG060400 Rh4DG060500 Rh4DG075600 Rh5CG423600
rosa_wichuraiana Rw2G053920 Rw2G053940 Rw2G053950 Rw2G054080 Rw2G054210 Rw4G000460 Rw4G005260 Rw4G005270 Rw4G005550 Rw4G006900 Rw5G036490 Rw5G036550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1014
AccB7I CCANNNNNTGG 1 cut(s) 120
AccI GTMKAC 1 cut(s) 876
AccIII TCCGGA 1 cut(s) 904
AciI CCGC 5 cut(s) 97, 157, 725, 738, 806
AclI AACGTT 1 cut(s) 413
AclWI GGATC 1 cut(s) 467
AcoI YGGCCR 1 cut(s) 775
AcsI RAATTY 4 cut(s) 178, 605, 795, 1150
AcuI CTGAAG 1 cut(s) 604
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 5 cut(s) 377, 514, 869, 1160, 1168
AfiI CCNNNNNNNGG 4 cut(s) 120, 547, 573, 734
AgsI TTSAA 2 cut(s) 103, 929
AluBI AGCT 3 cut(s) 26, 428, 894
AluI AGCT 3 cut(s) 26, 428, 894
Alw21I GWGCWC 1 cut(s) 636
Alw26I GTCTC 4 cut(s) 115, 193, 557, 1077
Alw44I GTGCAC 1 cut(s) 632
AlwI GGATC 1 cut(s) 467
Aor13HI TCCGGA 1 cut(s) 904
AoxI GGCC 3 cut(s) 139, 775, 803
ApaLI GTGCAC 1 cut(s) 632
ApeKI GCWGC 4 cut(s) 23, 348, 428, 985
ApoI RAATTY 4 cut(s) 178, 605, 795, 1150
ArsI GACNNNNNNTTYG 2 cut(s) 433, 465
AspS9I GGNCC 2 cut(s) 140, 207
AsuC2I CCSGG 2 cut(s) 205, 729
AsuHPI GGTGA 1 cut(s) 226
AvaII GGWCC 1 cut(s) 207
BaeGI GKGCMC 1 cut(s) 636
BalI TGGCCA 1 cut(s) 777
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 636
BbvCI CCTCAGC 1 cut(s) 359
BbvI GCAGC 4 cut(s) 35, 335, 415, 972
BccI CCATC 5 cut(s) 268, 741, 829, 904, 1080
BceAI ACGGC 3 cut(s) 349, 609, 751
BcgI CGANNNNNNTGC 2 cut(s) 866, 900
BcnI CCSGG 2 cut(s) 205, 729
BcoDI GTCTC 4 cut(s) 115, 193, 557, 1077
BfaI CTAG 1 cut(s) 801
BfmI CTRYAG 1 cut(s) 320
BisI GCNGC 6 cut(s) 24, 349, 429, 739, 806, 986
BlsI GCNGC 6 cut(s) 25, 350, 430, 740, 807, 987
Bme1390I CCNGG 2 cut(s) 205, 729
Bme18I GGWCC 1 cut(s) 207
BmgT120I GGNCC 2 cut(s) 140, 207
BmiI GGNNCC 1 cut(s) 142
BmrFI CCNGG 2 cut(s) 205, 729
BmsI GCATC 2 cut(s) 655, 793
BpiI GAAGAC 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 851
Bpu10I CCTNAGC 1 cut(s) 359
BpuEI CTTGAG 1 cut(s) 204
BpuMI CCSGG 2 cut(s) 205, 729
Bsa29I ATCGAT 1 cut(s) 485
BsaBI GATNNNNATC 2 cut(s) 699, 1090
BsaHI GRCGYC 1 cut(s) 309
BsaI GGTCTC 3 cut(s) 193, 557, 1077
BsaJI CCNNGG 3 cut(s) 183, 302, 625
BsaWI WCCGGW 2 cut(s) 368, 904
Bsc4I CCNNNNNNNGG 4 cut(s) 120, 547, 573, 734
Bse1I ACTGG 2 cut(s) 125, 399
Bse3DI GCAATG 1 cut(s) 646
Bse8I GATNNNNATC 2 cut(s) 699, 1090
BseAI TCCGGA 1 cut(s) 904
BseCI ATCGAT 1 cut(s) 485
BseDI CCNNGG 3 cut(s) 183, 302, 625
BseJI GATNNNNATC 2 cut(s) 699, 1090
BseLI CCNNNNNNNGG 4 cut(s) 120, 547, 573, 734
BseMI GCAATG 1 cut(s) 646
BseMII CTCAG 3 cut(s) 373, 513, 552
BseNI ACTGG 2 cut(s) 125, 399
BseRI GAGGAG 1 cut(s) 343
BseSI GKGCMC 1 cut(s) 636
BseXI GCAGC 4 cut(s) 35, 335, 415, 972
BsgI GTGCAG 1 cut(s) 971
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 3 cut(s) 141, 777, 805
BshVI ATCGAT 1 cut(s) 485
BsiEI CGRYCG 1 cut(s) 369
BsiHKAI GWGCWC 1 cut(s) 636
BsiSI CCGG 5 cut(s) 138, 205, 369, 728, 905
BslI CCNNNNNNNGG 4 cut(s) 120, 547, 573, 734
BsmAI GTCTC 4 cut(s) 115, 193, 557, 1077
BsmBI CGTCTC 1 cut(s) 115
BsnI GGCC 3 cut(s) 141, 777, 805
Bso31I GGTCTC 3 cut(s) 193, 557, 1077
Bsp1286I GDGCHC 1 cut(s) 636
Bsp13I TCCGGA 1 cut(s) 904
Bsp143I GATC 1 cut(s) 472
BspACI CCGC 5 cut(s) 97, 157, 725, 738, 806
BspANI GGCC 3 cut(s) 141, 777, 805
BspCNI CTCAG 3 cut(s) 372, 514, 553
BspDI ATCGAT 1 cut(s) 485
BspEI TCCGGA 1 cut(s) 904
BspLI GGNNCC 1 cut(s) 142
BspMAI CTGCAG 1 cut(s) 324
BspPI GGATC 1 cut(s) 467
BspTNI GGTCTC 3 cut(s) 193, 557, 1077
BsrDI GCAATG 1 cut(s) 646
BsrI ACTGG 2 cut(s) 125, 399
BssECI CCNNGG 3 cut(s) 183, 302, 625
BssMI GATC 1 cut(s) 472
BssNI GRCGYC 1 cut(s) 309
BssT1I CCWWGG 1 cut(s) 183
Bst4CI ACNGT 2 cut(s) 448, 715
BstACI GRCGYC 1 cut(s) 309
BstC8I GCNNGC 4 cut(s) 40, 77, 126, 338
BstDEI CTNAG 4 cut(s) 359, 522, 561, 1019
BstDSI CCRYGG 2 cut(s) 302, 625
BstEII GGTNACC 1 cut(s) 536
BstKTI GATC 1 cut(s) 475
BstMAI GTCTC 4 cut(s) 115, 193, 557, 1077
BstMBI GATC 1 cut(s) 472
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 3 cut(s) 200, 724, 814
BstNSI RCATGY 1 cut(s) 79
BstPI GGTNACC 1 cut(s) 536
BstSCI CCNGG 2 cut(s) 203, 727
BstSFI CTRYAG 1 cut(s) 320
BstSLI GKGCMC 1 cut(s) 636
BstV1I GCAGC 4 cut(s) 35, 335, 415, 972
BstV2I GAAGAC 1 cut(s) 54
Bsu15I ATCGAT 1 cut(s) 485
BsuRI GGCC 3 cut(s) 141, 777, 805
BsuTUI ATCGAT 1 cut(s) 485
BtgI CCRYGG 2 cut(s) 302, 625
BtgZI GCGATG 1 cut(s) 678
BtsI GCAGTG 1 cut(s) 634
BtsIMutI CAGTG 2 cut(s) 118, 634
Cac8I GCNNGC 4 cut(s) 40, 77, 126, 338
Cfr13I GGNCC 2 cut(s) 140, 207
ClaI ATCGAT 1 cut(s) 485
CseI GACGC 1 cut(s) 298
Csp6I GTAC 5 cut(s) 376, 513, 868, 1159, 1167
CviAII CATG 3 cut(s) 76, 161, 968
CviQI GTAC 5 cut(s) 376, 513, 868, 1159, 1167
DdeI CTNAG 4 cut(s) 359, 522, 561, 1019
DpnI GATC 1 cut(s) 474
DpnII GATC 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 775
EciI GGCGGA 1 cut(s) 714
Eco130I CCWWGG 1 cut(s) 183
Eco31I GGTCTC 3 cut(s) 193, 557, 1077
Eco47I GGWCC 1 cut(s) 207
Eco57I CTGAAG 1 cut(s) 604
Eco91I GGTNACC 1 cut(s) 536
EcoO65I GGTNACC 1 cut(s) 536
EcoT14I CCWWGG 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 183
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 3 cut(s) 79, 164, 971
FalI AAGNNNNNCTT 2 cut(s) 39, 71
FatI CATG 3 cut(s) 75, 160, 967
FauNDI CATATG 1 cut(s) 1107
FblI GTMKAC 1 cut(s) 876
Fnu4HI GCNGC 6 cut(s) 24, 349, 429, 739, 806, 986
Fsp4HI GCNGC 6 cut(s) 24, 349, 429, 739, 806, 986
FspBI CTAG 1 cut(s) 801
GluI GCNGC 6 cut(s) 24, 349, 429, 739, 806, 986
GsuI CTGGAG 1 cut(s) 851
HaeIII GGCC 3 cut(s) 141, 777, 805
HapII CCGG 5 cut(s) 138, 205, 369, 728, 905
HgaI GACGC 1 cut(s) 298
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 3 cut(s) 79, 164, 971
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HinfI GANTC 5 cut(s) 7, 316, 487, 524, 767
HpaII CCGG 5 cut(s) 138, 205, 369, 728, 905
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 3 cut(s) 634, 877, 1159
Hpy188I TCNGA 2 cut(s) 523, 562
Hpy188III TCNNGA 7 cut(s) 17, 221, 313, 709, 905, 926, 1130
Hpy8I GTNNAC 3 cut(s) 634, 877, 1159
Hpy99I CGWCG 3 cut(s) 254, 314, 509
HpyAV CCTTC 5 cut(s) 77, 205, 366, 571, 835
HpyCH4III ACNGT 2 cut(s) 448, 715
HpyCH4IV ACGT 2 cut(s) 381, 413
HpyF10VI GCNNNNNNNGC 3 cut(s) 200, 724, 814
HpyF3I CTNAG 4 cut(s) 359, 522, 561, 1019
HpySE526I ACGT 2 cut(s) 381, 413
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 3 cut(s) 79, 164, 971
Kpn2I TCCGGA 1 cut(s) 904
Kzo9I GATC 1 cut(s) 472
LmnI GCTCC 4 cut(s) 99, 278, 345, 356
Lsp1109I GCAGC 4 cut(s) 35, 335, 415, 972
LweI GCATC 2 cut(s) 655, 793
MaeI CTAG 1 cut(s) 801
MaeII ACGT 2 cut(s) 381, 413
MaeIII GTNAC 3 cut(s) 536, 731, 1037
MalI GATC 1 cut(s) 474
MboI GATC 1 cut(s) 472
MboII GAAGA 4 cut(s) 59, 190, 220, 223
MhlI GDGCHC 1 cut(s) 636
MlsI TGGCCA 1 cut(s) 777
MluCI AATT 9 cut(s) 178, 286, 542, 592, 605, 648, 795, 818, 1150
MluNI TGGCCA 1 cut(s) 777
MlyI GAGTC 3 cut(s) 16, 533, 776
MmeI TCCRAC 3 cut(s) 511, 554, 635
Mox20I TGGCCA 1 cut(s) 777
MroI TCCGGA 1 cut(s) 904
MscI TGGCCA 1 cut(s) 777
MseI TTAA 3 cut(s) 546, 882, 1043
MslI CAYNNNNRTG 1 cut(s) 639
Msp20I TGGCCA 1 cut(s) 777
MspA1I CMGCKG 2 cut(s) 26, 428
MspI CCGG 5 cut(s) 138, 205, 369, 728, 905
MspR9I CCNGG 2 cut(s) 205, 729
MwoI GCNNNNNNNGC 3 cut(s) 200, 724, 814
NciI CCSGG 2 cut(s) 205, 729
NdeI CATATG 1 cut(s) 1107
NdeII GATC 1 cut(s) 472
NlaIII CATG 3 cut(s) 79, 164, 971
NlaIV GGNNCC 1 cut(s) 142
NmeAIII GCCGAG 1 cut(s) 222
NmuCI GTSAC 2 cut(s) 731, 1037
NspI RCATGY 1 cut(s) 79
PaeI GCATGC 1 cut(s) 79
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PfeI GAWTC 2 cut(s) 316, 487
PflMI CCANNNNNTGG 1 cut(s) 120
PkrI GCNGC 6 cut(s) 25, 350, 430, 740, 807, 987
PleI GAGTC 3 cut(s) 15, 532, 775
PpsI GAGTC 3 cut(s) 15, 532, 775
PsiI TTATAA 1 cut(s) 1014
Psp1406I AACGTT 1 cut(s) 413
PspEI GGTNACC 1 cut(s) 536
PspN4I GGNNCC 1 cut(s) 142
PspPI GGNCC 2 cut(s) 140, 207
PstI CTGCAG 1 cut(s) 324
PvuII CAGCTG 2 cut(s) 26, 428
RsaI GTAC 5 cut(s) 377, 514, 869, 1160, 1168
RsaNI GTAC 5 cut(s) 376, 513, 868, 1159, 1167
RseI CAYNNNNRTG 1 cut(s) 639
SalI GTCGAC 1 cut(s) 875
SaqAI TTAA 3 cut(s) 546, 882, 1043
SatI GCNGC 6 cut(s) 24, 349, 429, 739, 806, 986
Sau3AI GATC 1 cut(s) 472
Sau96I GGNCC 2 cut(s) 140, 207
SchI GAGTC 3 cut(s) 16, 533, 776
ScrFI CCNGG 2 cut(s) 205, 729
SduI GDGCHC 1 cut(s) 636
SfaNI GCATC 2 cut(s) 655, 793
SfcI CTRYAG 1 cut(s) 320
SinI GGWCC 1 cut(s) 207
SmiMI CAYNNNNRTG 1 cut(s) 639
SmlI CTYRAG 1 cut(s) 219
SmoI CTYRAG 1 cut(s) 219
SphI GCATGC 1 cut(s) 79
Sse9I AATT 9 cut(s) 178, 286, 542, 592, 605, 648, 795, 818, 1150
SsiI CCGC 5 cut(s) 97, 157, 725, 738, 806
SspMI CTAG 1 cut(s) 801
StyD4I CCNGG 2 cut(s) 203, 727
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 2 cut(s) 448, 715
TaiI ACGT 2 cut(s) 384, 416
TaqI TCGA 8 cut(s) 312, 440, 471, 485, 609, 792, 876, 1084
TasI AATT 9 cut(s) 178, 286, 542, 592, 605, 648, 795, 818, 1150
TatI WGTACW 3 cut(s) 867, 1158, 1166
TauI GCSGC 2 cut(s) 741, 808
TfiI GAWTC 2 cut(s) 316, 487
Tru1I TTAA 3 cut(s) 546, 882, 1043
Tru9I TTAA 3 cut(s) 546, 882, 1043
TscAI CASTG 2 cut(s) 125, 641
TseFI GTSAC 2 cut(s) 731, 1037
TseI GCWGC 4 cut(s) 23, 348, 428, 985
Tsp45I GTSAC 2 cut(s) 731, 1037
TspDTI ATGAA 3 cut(s) 479, 629, 711
TspGWI ACGGA 1 cut(s) 97
TspRI CASTG 2 cut(s) 125, 641
Van91I CCANNNNNTGG 1 cut(s) 120
VneI GTGCAC 1 cut(s) 632
VpaK11BI GGWCC 1 cut(s) 207
XapI RAATTY 4 cut(s) 178, 605, 795, 1150
XceI RCATGY 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 876
XspI CTAG 1 cut(s) 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.