Rw4G000460

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000237
Physical Location & Seq
Forward (+)
1063609 .. 1066093
2485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G000460.1

Sequence Viewer

Length: 1092 bp
ATGATACAGCCACTAAAACGCTGCGGCAGCAGCTTACCAGAAACCATATTAGACTATGAAGATATCCTTACGGAAATCCTTGTGCGTGTGCCAGCTCGAACTCTGGTCCGCTTCAAATGCGTCTCCAAGCATTGGCTCTCTCTTATCTCCAACCCCAATTTCTGTCACCTCCACACCCTACGAAATCCTCCACACTCCTCCATCTCCGCCTTCTTTGCTAGAACATCCAGTGAGTTTTGTCTCGTCCCTCTTGACCTTGATCATGGTCAGACTAGTAATAGTAACTGTAATCCTCTGAATTTTGGTCACAACCTATATGCTATTGAGATTGTCCAGTCCTGCAATGGCCTCTTCTTGTGCTGTCCCCTACCTGCAGAAGACTCTTCATTCTCCACCACTACAACTAGTAAACCTCTTTATTATGTTCTCAATCCTACAACCAACCAGTTCTCCGCACTTACTCCTCCAGCTGCTGCTGCTGCCACTACTGGTATACCCAGAGTCTTCGGCTGTGCCCTGGCTTTTGACCCTTCCAAATCACCTCATTACAAAGTGGTCTTCATTTGGTGCGTCAATGAACCAATTCAGGCTGGCTGGTGCCCGTACCATCACATAGAGATATATTCGTCTGAGACTCGAAGTTGGAGGCTTCTCGATTCTACTTTCGACACTCAACCTCAAGTCCGCTACGAAGAGGGAGTATACTGCAATGGCGCAGTTCATTGGGTGGGCATTGATTGTGAGATGTCATACTATCACATAGATGAAGAACGTGTTGGATTGGTTGATGGTTTCCCTAAGAGTCACGAGAAGAATTTGGAAACGAGGCTGTCTAGATATTTTCGGGAGTCTCATGGTGGTGGCCATTTGCATCTTATTGATATTTATGGATATTCTCTTACTAAATTTGAAGTCATGGAGATGGGGAGAGACTACTCTGGCTGGTTTGTCAAGTACAATGTTGATCTTGATCCCTTATGCACCACTTCCCCGCAGATCCTACGTGCTATTTTTGTTCTTCTCACTCTTGCTCCGGAGGAAAATGAAGAAGATGAGGAAAGTTCATCTCTGTTGCTGCATACTCCTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

363

Amino Acids

41.01

Weight (kDa)

5.47

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 20 - 56 2.4e-07 F-box domain
FBA_1 PF07734 73 - 246 5.9e-13 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000237)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14315
fragaria_vesca FvH4_2g35920 FvH4_2g35930 FvH4_2g35940 FvH4_2g35950 FvH4_3g00321 FvH4_3g32490 FvH4_3g43790 FvH4_3g43790 FvH4_3g43790 FvH4_4g06360 FvH4_4g06370 FvH4_4g06370 FvH4_6g52062 FvH4_6g52062 FvH4_6g52080 FvH4_6g52610 FvH4_6g52830 FvH4_6g52861 FvH4_6g52861 FvH4_6g52862 FvH4_6g52871 FvH4_6g52880 FvH4_6g52931 FvH4_6g52941 FvH4_6g52942 FvH4_6g53001 FvH4_6g53021 FvH4_6g53072 FvH4_6g53610 FvH4_6g53620 FvH4_6g53620 FvH4_6g53651 FvH4_6g53660
malus_domestica MD13G1235400.v1.1 MD16G1240000.v1.1
prunus_persica Prupe.1G066400_v2.0.a1
pyrus_communis pycom13g20790 pycom16g20090
rosa_chinensis RchiOBHm_Chr2g0174451 RchiOBHm_Chr2g0174531 RchiOBHm_Chr2g0174701 RchiOBHm_Chr2g0174881 RchiOBHm_Chr2g0174951 RchiOBHm_Chr4g0396041 RchiOBHm_Chr4g0396501 RchiOBHm_Chr4g0398111 RchiOBHm_Chr5g0059531 RchiOBHm_Chr6g0264241
rosa_laevigata RLG00000009385 RLG00000009528 RLG00000009532 RLG00000009537 RLG00000009541 RLG00000009577 RLG00000022267 RLG00000022268 RLG00000022269 RLG00000022273 RLG00000022287 RLG00000022289 RLG00000032767 RLG00000035297
rosa_multiflora Rmu_co8114622.1_g000001 Rmu_co8160300.1_g000001 Rmu_co8411703.1_g000001 Rmu_sc0001217.1_g000015 Rmu_sc0001533.1_g000002 Rmu_sc0003649.1_g000012 Rmu_sc0003649.1_g000015 Rmu_sc0003649.1_g000017 Rmu_sc0003649.1_g000020 Rmu_sc0006325.1_g000006 Rmu_sc0009506.1_g000001 Rmu_sc0009766.1_g000006 Rmu_sc0015078.1_g000002 Rmu_sc0015594.1_g000006 Rmu_sc0015594.1_g000007
rosa_roxburghii Rroxscaffold_1G00021050 Rroxscaffold_2G00077800 Rroxscaffold_2G00077920 Rroxscaffold_2G00078070 Rroxscaffold_2G00078100 Rroxscaffold_2G00078130 Rroxscaffold_2G00078170 Rroxscaffold_3G00220050 Rroxscaffold_5G00340840 Rroxscaffold_5G00340850 Rroxscaffold_5G00341370 Rroxscaffold_5G00343010 Rroxscaffold_5G00381750
rosa_rugosa Rorug02G0577300 Rorug02G0577400 Rorug02G0577700 Rorug02G0577800 Rorug02G0579000 Rorug03G0352900 Rorug03G0352900 Rorug04G0006000 Rorug05G0323000
rosa_samantha Rh2AG658100 Rh2AG658400 Rh2AG658600 Rh2AG660200 Rh2AG663100 Rh2CG632100 Rh2CG632600 Rh2CG632700 Rh2CG634300 Rh2CG635600 Rh2DG682800 Rh2DG683300 Rh2DG686000 Rh3BG068500 Rh3CG066900 Rh4AG064800 Rh4AG065900 Rh4AG068900 Rh4AG083000 Rh4BG063600 Rh4BG063700 Rh4BG067000 Rh4BG080700 Rh4DG060400 Rh4DG060500 Rh4DG075600 Rh5CG423600
rosa_wichuraiana Rw2G053920 Rw2G053940 Rw2G053950 Rw2G054080 Rw2G054210 Rw4G000460 Rw4G005260 Rw4G005270 Rw4G005550 Rw4G006900 Rw5G036490 Rw5G036550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 379
AccB1I GGYRCC 1 cut(s) 597
AccB7I CCANNNNNTGG 1 cut(s) 132
AccI GTMKAC 2 cut(s) 493, 702
AccIII TCCGGA 1 cut(s) 1033
AciI CCGC 6 cut(s) 24, 109, 207, 453, 685, 992
AclWI GGATC 2 cut(s) 965, 991
AcoI YGGCCR 1 cut(s) 862
AcsI RAATTY 3 cut(s) 298, 814, 905
AfaI GTAC 2 cut(s) 605, 956
AfiI CCNNNNNNNGG 1 cut(s) 132
AflIII ACRYGT 1 cut(s) 772
AgsI TTSAA 2 cut(s) 115, 911
AhlI ACTAGT 2 cut(s) 272, 404
AjnI CCWGG 2 cut(s) 516, 1084
AjuI GAANNNNNNNTTGG 4 cut(s) 143, 175, 759, 791
AluBI AGCT 3 cut(s) 33, 95, 470
AluI AGCT 3 cut(s) 33, 95, 470
Alw26I GTCTC 5 cut(s) 127, 245, 626, 855, 924
AlwI GGATC 2 cut(s) 965, 991
AlwNI CAGNNNCTG 1 cut(s) 473
Aor13HI TCCGGA 1 cut(s) 1033
AoxI GGCC 2 cut(s) 346, 862
ApeKI GCWGC 8 cut(s) 21, 27, 30, 470, 473, 476, 479, 1075
ApoI RAATTY 3 cut(s) 298, 814, 905
Asp700I GAANNNNTTC 1 cut(s) 582
AspLEI GCGC 1 cut(s) 716
AspS9I GGNCC 1 cut(s) 106
AsuHPI GGTGA 2 cut(s) 158, 531
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 2 cut(s) 517, 602
BalI TGGCCA 1 cut(s) 864
BanI GGYRCC 1 cut(s) 597
BauI CACGAG 1 cut(s) 806
BbsI GAAGAC 3 cut(s) 384, 496, 550
BbvI GCAGC 8 cut(s) 8, 39, 42, 457, 460, 463, 466, 1062
BccI CCATC 4 cut(s) 209, 615, 782, 916
BciT130I CCWGG 2 cut(s) 518, 1086
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 5 cut(s) 127, 245, 626, 855, 924
BcuI ACTAGT 2 cut(s) 272, 404
BfaI CTAG 4 cut(s) 219, 273, 405, 834
BfmI CTRYAG 1 cut(s) 372
BfuAI ACCTGC 1 cut(s) 379
BisI GCNGC 9 cut(s) 22, 25, 28, 31, 471, 474, 477, 480, 1076
BlsI GCNGC 9 cut(s) 23, 26, 29, 32, 472, 475, 478, 481, 1077
Bme1390I CCNGG 2 cut(s) 518, 1086
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 1 cut(s) 599
BmrFI CCNGG 2 cut(s) 518, 1086
BmsI GCATC 1 cut(s) 880
BpiI GAAGAC 3 cut(s) 384, 496, 550
BpmI CTGGAG 1 cut(s) 450
BpuEI CTTGAG 1 cut(s) 663
BsaAI YACGTR 1 cut(s) 1004
BsaBI GATNNNNATC 1 cut(s) 969
BsaJI CCNNGG 2 cut(s) 516, 1085
BsaWI WCCGGW 1 cut(s) 1033
BsaXI ACNNNNNCTCC 4 cut(s) 434, 464, 1015, 1045
Bsc4I CCNNNNNNNGG 1 cut(s) 132
Bse1I ACTGG 4 cut(s) 228, 334, 445, 493
Bse3DI GCAATG 2 cut(s) 349, 715
Bse8I GATNNNNATC 1 cut(s) 969
BseAI TCCGGA 1 cut(s) 1033
BseBI CCWGG 2 cut(s) 518, 1086
BseDI CCNNGG 2 cut(s) 516, 1085
BseGI GGATG 1 cut(s) 224
BseJI GATNNNNATC 1 cut(s) 969
BseLI CCNNNNNNNGG 1 cut(s) 132
BseMI GCAATG 2 cut(s) 349, 715
BseMII CTCAG 1 cut(s) 621
BseNI ACTGG 4 cut(s) 228, 334, 445, 493
BseRI GAGGAG 2 cut(s) 187, 453
BseSI GKGCMC 2 cut(s) 517, 602
BseXI GCAGC 8 cut(s) 8, 39, 42, 457, 460, 463, 466, 1062
BshFI GGCC 2 cut(s) 348, 864
BshNI GGYRCC 1 cut(s) 597
BsiSI CCGG 1 cut(s) 1034
BslFI GGGAC 2 cut(s) 230, 348
BslI CCNNNNNNNGG 1 cut(s) 132
BsmAI GTCTC 5 cut(s) 127, 245, 626, 855, 924
BsmBI CGTCTC 1 cut(s) 127
BsmFI GGGAC 2 cut(s) 230, 348
BsnI GGCC 2 cut(s) 348, 864
Bsp1286I GDGCHC 2 cut(s) 517, 602
Bsp13I TCCGGA 1 cut(s) 1033
Bsp143I GATC 4 cut(s) 259, 964, 970, 996
BspACI CCGC 6 cut(s) 24, 109, 207, 453, 685, 992
BspANI GGCC 2 cut(s) 348, 864
BspCNI CTCAG 1 cut(s) 622
BspEI TCCGGA 1 cut(s) 1033
BspLI GGNNCC 1 cut(s) 599
BspMAI CTGCAG 1 cut(s) 376
BspMI ACCTGC 1 cut(s) 379
BspPI GGATC 2 cut(s) 965, 991
BspT107I GGYRCC 1 cut(s) 597
BsrDI GCAATG 2 cut(s) 349, 715
BsrI ACTGG 4 cut(s) 228, 334, 445, 493
BssECI CCNNGG 2 cut(s) 516, 1085
BssMI GATC 4 cut(s) 259, 964, 970, 996
BssNAI GTATAC 2 cut(s) 494, 703
BssSI CACGAG 1 cut(s) 806
Bst1107I GTATAC 2 cut(s) 494, 703
Bst2BI CACGAG 1 cut(s) 806
Bst2UI CCWGG 2 cut(s) 518, 1086
Bst4CI ACNGT 1 cut(s) 287
Bst6I CTCTTC 3 cut(s) 356, 388, 687
BstBAI YACGTR 1 cut(s) 1004
BstC8I GCNNGC 2 cut(s) 93, 592
BstDEI CTNAG 2 cut(s) 630, 798
BstF5I GGATG 1 cut(s) 224
BstHHI GCGC 1 cut(s) 716
BstKTI GATC 4 cut(s) 262, 967, 973, 999
BstMAI GTCTC 5 cut(s) 127, 245, 626, 855, 924
BstMBI GATC 4 cut(s) 259, 964, 970, 996
BstMWI GCNNNNNNNGC 6 cut(s) 27, 30, 117, 215, 476, 479
BstNI CCWGG 2 cut(s) 518, 1086
BstSCI CCNGG 2 cut(s) 516, 1084
BstSFI CTRYAG 1 cut(s) 372
BstSLI GKGCMC 2 cut(s) 517, 602
BstV1I GCAGC 8 cut(s) 8, 39, 42, 457, 460, 463, 466, 1062
BstV2I GAAGAC 3 cut(s) 384, 496, 550
BstX2I RGATCY 1 cut(s) 996
BstYI RGATCY 1 cut(s) 996
BstZ17I GTATAC 2 cut(s) 494, 703
BsuRI GGCC 2 cut(s) 348, 864
BtsCI GGATG 1 cut(s) 224
BtsIMutI CAGTG 1 cut(s) 235
BveI ACCTGC 1 cut(s) 379
Cac8I GCNNGC 2 cut(s) 93, 592
CaiI CAGNNNCTG 1 cut(s) 473
CfoI GCGC 1 cut(s) 716
Cfr13I GGNCC 1 cut(s) 106
CseI GACGC 2 cut(s) 109, 559
Csp6I GTAC 2 cut(s) 604, 955
CviAII CATG 3 cut(s) 263, 854, 916
CviQI GTAC 2 cut(s) 604, 955
DdeI CTNAG 2 cut(s) 630, 798
DpnI GATC 4 cut(s) 261, 966, 972, 998
DpnII GATC 4 cut(s) 259, 964, 970, 996
EaeI YGGCCR 1 cut(s) 862
Eam1104I CTCTTC 3 cut(s) 356, 388, 687
EarI CTCTTC 3 cut(s) 356, 388, 687
EciI GGCGGA 1 cut(s) 196
Eco32I GATATC 1 cut(s) 64
Eco47I GGWCC 1 cut(s) 106
EcoRII CCWGG 2 cut(s) 516, 1084
EcoRV GATATC 1 cut(s) 64
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 3 cut(s) 266, 857, 919
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FaqI GGGAC 2 cut(s) 230, 348
FatI CATG 3 cut(s) 262, 853, 915
FauI CCCGC 1 cut(s) 999
FbaI TGATCA 1 cut(s) 259
FblI GTMKAC 2 cut(s) 493, 702
Fnu4HI GCNGC 9 cut(s) 22, 25, 28, 31, 471, 474, 477, 480, 1076
FokI GGATG 1 cut(s) 211
Fsp4HI GCNGC 9 cut(s) 22, 25, 28, 31, 471, 474, 477, 480, 1076
FspBI CTAG 4 cut(s) 219, 273, 405, 834
GlaI GCGC 1 cut(s) 715
GluI GCNGC 9 cut(s) 22, 25, 28, 31, 471, 474, 477, 480, 1076
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 2 cut(s) 348, 864
HapII CCGG 1 cut(s) 1034
HgaI GACGC 2 cut(s) 109, 559
HhaI GCGC 1 cut(s) 716
Hin1II CATG 3 cut(s) 266, 857, 919
Hin6I GCGC 1 cut(s) 714
HinP1I GCGC 1 cut(s) 714
HinfI GANTC 6 cut(s) 380, 501, 634, 656, 802, 848
HpaII CCGG 1 cut(s) 1034
HphI GGTGA 2 cut(s) 158, 531
Hpy166II GTNNAC 3 cut(s) 410, 494, 703
Hpy188I TCNGA 3 cut(s) 270, 297, 631
Hpy188III TCNNGA 7 cut(s) 251, 653, 806, 834, 845, 968, 1034
Hpy8I GTNNAC 3 cut(s) 410, 494, 703
HpyAV CCTTC 2 cut(s) 220, 540
HpyCH4III ACNGT 1 cut(s) 287
HpyCH4IV ACGT 2 cut(s) 772, 1003
HpyCH4V TGCA 6 cut(s) 342, 374, 708, 871, 981, 1078
HpyF10VI GCNNNNNNNGC 6 cut(s) 27, 30, 117, 215, 476, 479
HpyF3I CTNAG 2 cut(s) 630, 798
HpySE526I ACGT 2 cut(s) 772, 1003
Hsp92II CATG 3 cut(s) 266, 857, 919
HspAI GCGC 1 cut(s) 714
Kpn2I TCCGGA 1 cut(s) 1033
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 4 cut(s) 259, 964, 970, 996
LmnI GCTCC 1 cut(s) 1036
Lsp1109I GCAGC 8 cut(s) 8, 39, 42, 457, 460, 463, 466, 1062
LweI GCATC 1 cut(s) 880
MaeI CTAG 4 cut(s) 219, 273, 405, 834
MaeII ACGT 2 cut(s) 772, 1003
MaeIII GTNAC 4 cut(s) 164, 281, 305, 803
MalI GATC 4 cut(s) 261, 966, 972, 998
MboI GATC 4 cut(s) 259, 964, 970, 996
MflI RGATCY 1 cut(s) 996
MhlI GDGCHC 2 cut(s) 517, 602
MlsI TGGCCA 1 cut(s) 864
MluCI AATT 5 cut(s) 157, 298, 582, 814, 905
MluNI TGGCCA 1 cut(s) 864
MlyI GAGTC 5 cut(s) 374, 510, 628, 811, 857
MmeI TCCRAC 3 cut(s) 174, 623, 757
Mox20I TGGCCA 1 cut(s) 864
MroI TCCGGA 1 cut(s) 1033
MroXI GAANNNNTTC 1 cut(s) 582
MscI TGGCCA 1 cut(s) 864
MslI CAYNNNNRTG 3 cut(s) 762, 858, 920
Msp20I TGGCCA 1 cut(s) 864
MspA1I CMGCKG 1 cut(s) 470
MspI CCGG 1 cut(s) 1034
MspR9I CCNGG 2 cut(s) 518, 1086
MvaI CCWGG 2 cut(s) 518, 1086
MwoI GCNNNNNNNGC 6 cut(s) 27, 30, 117, 215, 476, 479
NdeII GATC 4 cut(s) 259, 964, 970, 996
NlaIII CATG 3 cut(s) 266, 857, 919
NlaIV GGNNCC 1 cut(s) 599
NmuCI GTSAC 3 cut(s) 164, 305, 803
PdmI GAANNNNTTC 1 cut(s) 582
PfeI GAWTC 1 cut(s) 656
PflMI CCANNNNNTGG 1 cut(s) 132
PkrI GCNGC 9 cut(s) 23, 26, 29, 32, 472, 475, 478, 481, 1077
PleI GAGTC 5 cut(s) 374, 509, 628, 810, 856
PpsI GAGTC 5 cut(s) 374, 509, 628, 810, 856
Ppu21I YACGTR 1 cut(s) 1004
Psp6I CCWGG 2 cut(s) 516, 1084
PspGI CCWGG 2 cut(s) 516, 1084
PspN4I GGNNCC 1 cut(s) 599
PspPI GGNCC 1 cut(s) 106
PstI CTGCAG 1 cut(s) 376
PstNI CAGNNNCTG 1 cut(s) 473
PsuI RGATCY 1 cut(s) 996
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 2 cut(s) 605, 956
RsaNI GTAC 2 cut(s) 604, 955
RseI CAYNNNNRTG 3 cut(s) 762, 858, 920
SatI GCNGC 9 cut(s) 22, 25, 28, 31, 471, 474, 477, 480, 1076
Sau3AI GATC 4 cut(s) 259, 964, 970, 996
Sau96I GGNCC 1 cut(s) 106
SchI GAGTC 5 cut(s) 374, 510, 628, 811, 857
ScrFI CCNGG 2 cut(s) 518, 1086
SduI GDGCHC 2 cut(s) 517, 602
SfaNI GCATC 1 cut(s) 880
SfcI CTRYAG 1 cut(s) 372
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 3 cut(s) 762, 858, 920
SmlI CTYRAG 1 cut(s) 678
SmoI CTYRAG 1 cut(s) 678
SpeI ACTAGT 2 cut(s) 272, 404
Sse9I AATT 5 cut(s) 157, 298, 582, 814, 905
SsiI CCGC 6 cut(s) 24, 109, 207, 453, 685, 992
SspMI CTAG 4 cut(s) 219, 273, 405, 834
StyD4I CCNGG 2 cut(s) 516, 1084
TaaI ACNGT 1 cut(s) 287
TaiI ACGT 2 cut(s) 775, 1006
TaqI TCGA 4 cut(s) 97, 637, 654, 666
TasI AATT 5 cut(s) 157, 298, 582, 814, 905
TatI WGTACW 1 cut(s) 954
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 1 cut(s) 656
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 3 cut(s) 164, 305, 803
TseI GCWGC 8 cut(s) 21, 27, 30, 470, 473, 476, 479, 1075
Tsp45I GTSAC 3 cut(s) 164, 305, 803
TspDTI ATGAA 8 cut(s) 72, 375, 550, 591, 710, 780, 1053, 1059
TspGWI ACGGA 1 cut(s) 86
TspRI CASTG 1 cut(s) 235
Van91I CCANNNNNTGG 1 cut(s) 132
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 3 cut(s) 298, 814, 905
XbaI TCTAGA 1 cut(s) 833
XcmI CCANNNNNNNNNTGG 1 cut(s) 341
XmiI GTMKAC 2 cut(s) 493, 702
XmnI GAANNNNTTC 1 cut(s) 582
XspI CTAG 4 cut(s) 219, 273, 405, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.