RLG00000009532

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000237
Physical Location & Seq
Forward (+)
54116385 .. 54118035
1651 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009532

Sequence Viewer

Length: 1218 bp
ATGAAGTCGAACAGAATTAATATGGTTCAGGGATTCTATTTGAAACCGAGTATTGCCAGTAGTAGCACTAGCAGCAGCAGTATCAGCTTAGCAGAAATCATAGCCAGCACGGAGGAACTCCTTATGGAAATCCTACTGCGCGTGCCAGCTCGAACTCTGGTCCGCTTCAAATGTGTTTCCAAGCATTGGCTCGCTCTTATATCTGACCCCAAATTCTGTCACCGCCACACCCTTCGGAACCAAAACTCTTCCATCTCTGCAGTCTTTGGTGACAGATCCATAGACTTTTTTTCCATCATTTTTTCTCTTGATCATGATCGTAGGGGTAGTAACCATGGAAACCATAACCCCCGTCCATCACGCTGCAATCCTCTTAATTTTGTTCAAAACCAACATGATCCCATCGAGATTATCCACTCCTGCAATGGCCTCTTCTTGTGTCGTCCCATCAATGCTACAAGTACTAGTAGAACCCCATATTTTGTTCTTAATCCCACAACCAATCAGTTCTTGACGCTTACAATTCCCCCAGCTGCTGCTGCTACTTCTAATGGTCAACAGATACAAACCCATATTATTGGTTGTGCTGTGGCTTTTGACCCTTCCAAATCACCTCATTACAAGGTTATATGCCTTGAGACCACTACTGGTCATAAATATTGCCCATACTACCAAATACAAATATATTCATCAGAGACTCAAAGTTGGAGGCTTCTCAGTTCTACTTTTATAAGGCAAGATCAAACTCTCTATGAACAGGGGGTATATTGGAACGGCGCAATTCATTGGATAGGCCTGGTTTGTGAGATGTCATACTATCAAGTAGATGAAGAGCTTGTCGGATTGGTTGATAGTCCTCCTCATTGTTACGAGAAGAAATGGAACCATAGGATGTATAGATATTTTAAGGAGTCTACTGGCGGCCATTTGCATCTTATTGATATTTATTCTCCTTGTCCTACTAAATTTGAAGTATTGGAAATGGGGAGAGATTACTCTGGCTGGTTTGTCAAGTACCATGTTGATCTTGATCCCTTATGCACCGCTTACCCTTGCTTCCTAAGTGAACAGTTTGTTGTTCTGTTCCTTGCTCAAGATGAAAATGAAGGGGAAAGTTCATCCCTGTTGCTGCATTCTCCTGATAATGTCGTTGAGATTGAGGTTGTTACTCCTCCATCCAGCGTGAGTGCATGGCCAATCAAGGTAGTGGTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

406

Amino Acids

46.07

Weight (kDa)

6.4

Isoelectric Point (pI)

45.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 38 - 74 1.1e-07 F-box domain
FBA_3 PF08268 124 - 267 7.6e-09 F-box associated beta propeller domain
FBA_1 PF07734 128 - 265 2.2e-11 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 135 - 265 4.9e-06 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000237)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14315
fragaria_vesca FvH4_2g35920 FvH4_2g35930 FvH4_2g35940 FvH4_2g35950 FvH4_3g00321 FvH4_3g32490 FvH4_3g43790 FvH4_3g43790 FvH4_3g43790 FvH4_4g06360 FvH4_4g06370 FvH4_4g06370 FvH4_6g52062 FvH4_6g52062 FvH4_6g52080 FvH4_6g52610 FvH4_6g52830 FvH4_6g52861 FvH4_6g52861 FvH4_6g52862 FvH4_6g52871 FvH4_6g52880 FvH4_6g52931 FvH4_6g52941 FvH4_6g52942 FvH4_6g53001 FvH4_6g53021 FvH4_6g53072 FvH4_6g53610 FvH4_6g53620 FvH4_6g53620 FvH4_6g53651 FvH4_6g53660
malus_domestica MD13G1235400.v1.1 MD16G1240000.v1.1
prunus_persica Prupe.1G066400_v2.0.a1
pyrus_communis pycom13g20790 pycom16g20090
rosa_chinensis RchiOBHm_Chr2g0174451 RchiOBHm_Chr2g0174531 RchiOBHm_Chr2g0174701 RchiOBHm_Chr2g0174881 RchiOBHm_Chr2g0174951 RchiOBHm_Chr4g0396041 RchiOBHm_Chr4g0396501 RchiOBHm_Chr4g0398111 RchiOBHm_Chr5g0059531 RchiOBHm_Chr6g0264241
rosa_laevigata RLG00000009385 RLG00000009528 RLG00000009532 RLG00000009537 RLG00000009541 RLG00000009577 RLG00000022267 RLG00000022268 RLG00000022269 RLG00000022273 RLG00000022287 RLG00000022289 RLG00000032767 RLG00000035297
rosa_multiflora Rmu_co8114622.1_g000001 Rmu_co8160300.1_g000001 Rmu_co8411703.1_g000001 Rmu_sc0001217.1_g000015 Rmu_sc0001533.1_g000002 Rmu_sc0003649.1_g000012 Rmu_sc0003649.1_g000015 Rmu_sc0003649.1_g000017 Rmu_sc0003649.1_g000020 Rmu_sc0006325.1_g000006 Rmu_sc0009506.1_g000001 Rmu_sc0009766.1_g000006 Rmu_sc0015078.1_g000002 Rmu_sc0015594.1_g000006 Rmu_sc0015594.1_g000007
rosa_roxburghii Rroxscaffold_1G00021050 Rroxscaffold_2G00077800 Rroxscaffold_2G00077920 Rroxscaffold_2G00078070 Rroxscaffold_2G00078100 Rroxscaffold_2G00078130 Rroxscaffold_2G00078170 Rroxscaffold_3G00220050 Rroxscaffold_5G00340840 Rroxscaffold_5G00340850 Rroxscaffold_5G00341370 Rroxscaffold_5G00343010 Rroxscaffold_5G00381750
rosa_rugosa Rorug02G0577300 Rorug02G0577400 Rorug02G0577700 Rorug02G0577800 Rorug02G0579000 Rorug03G0352900 Rorug03G0352900 Rorug04G0006000 Rorug05G0323000
rosa_samantha Rh2AG658100 Rh2AG658400 Rh2AG658600 Rh2AG660200 Rh2AG663100 Rh2CG632100 Rh2CG632600 Rh2CG632700 Rh2CG634300 Rh2CG635600 Rh2DG682800 Rh2DG683300 Rh2DG686000 Rh3BG068500 Rh3CG066900 Rh4AG064800 Rh4AG065900 Rh4AG068900 Rh4AG083000 Rh4BG063600 Rh4BG063700 Rh4BG067000 Rh4BG080700 Rh4DG060400 Rh4DG060500 Rh4DG075600 Rh5CG423600
rosa_wichuraiana Rw2G053920 Rw2G053940 Rw2G053950 Rw2G054080 Rw2G054210 Rw4G000460 Rw4G005260 Rw4G005270 Rw4G005550 Rw4G006900 Rw5G036490 Rw5G036550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 731
AccB7I CCANNNNNTGG 1 cut(s) 186
AccI GTMKAC 1 cut(s) 914
AccII CGCG 1 cut(s) 141
AciI CCGC 4 cut(s) 163, 223, 921, 1044
AclWI GGATC 3 cut(s) 270, 392, 1025
AcoI YGGCCR 2 cut(s) 922, 1193
AcsI RAATTY 2 cut(s) 212, 965
AfaI GTAC 2 cut(s) 463, 1016
AfiI CCNNNNNNNGG 1 cut(s) 186
AgsI TTSAA 4 cut(s) 43, 169, 386, 971
AhlI ACTAGT 1 cut(s) 464
AjnI CCWGG 1 cut(s) 795
AluBI AGCT 4 cut(s) 87, 149, 533, 835
AluI AGCT 4 cut(s) 87, 149, 533, 835
Alw26I GTCTC 2 cut(s) 632, 689
AlwI GGATC 3 cut(s) 270, 392, 1025
AlwNI CAGNNNCTG 1 cut(s) 536
AoxI GGCC 4 cut(s) 427, 793, 922, 1193
ApeKI GCWGC 7 cut(s) 72, 75, 363, 533, 536, 539, 1129
ApoI RAATTY 2 cut(s) 212, 965
AseI ATTAAT 1 cut(s) 18
AspLEI GCGC 2 cut(s) 141, 779
AspS9I GGNCC 1 cut(s) 160
AsuHPI GGTGA 3 cut(s) 212, 281, 603
AvaII GGWCC 1 cut(s) 160
BalI TGGCCA 1 cut(s) 1195
BbvI GCAGC 7 cut(s) 84, 87, 350, 520, 523, 526, 1116
BccI CCATC 6 cut(s) 260, 302, 364, 410, 455, 1183
BceAI ACGGC 1 cut(s) 790
BciT130I CCWGG 1 cut(s) 797
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 2 cut(s) 632, 689
BcuI ACTAGT 1 cut(s) 464
BfaI CTAG 2 cut(s) 69, 465
BfmI CTRYAG 1 cut(s) 258
BisI GCNGC 8 cut(s) 73, 76, 364, 534, 537, 540, 922, 1130
BlpI GCTNAGC 1 cut(s) 88
BlsI GCNGC 8 cut(s) 74, 77, 365, 535, 538, 541, 923, 1131
BmcAI AGTACT 1 cut(s) 463
Bme1390I CCNGG 1 cut(s) 797
Bme18I GGWCC 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 160
BmiI GGNNCC 2 cut(s) 239, 884
BmrFI CCNGG 1 cut(s) 797
BmsI GCATC 1 cut(s) 940
Bpu1102I GCTNAGC 1 cut(s) 88
BpuEI CTTGAG 2 cut(s) 656, 1077
BsaBI GATNNNNATC 2 cut(s) 315, 1029
BsaI GGTCTC 1 cut(s) 632
BsaJI CCNNGG 1 cut(s) 334
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse1I ACTGG 3 cut(s) 57, 652, 922
Bse3DI GCAATG 1 cut(s) 430
Bse8I GATNNNNATC 2 cut(s) 315, 1029
BseBI CCWGG 1 cut(s) 797
BseDI CCNNGG 1 cut(s) 334
BseGI GGATG 3 cut(s) 897, 1118, 1175
BseJI GATNNNNATC 2 cut(s) 315, 1029
BseLI CCNNNNNNNGG 1 cut(s) 186
BseMI GCAATG 1 cut(s) 430
BseMII CTCAG 1 cut(s) 730
BseNI ACTGG 3 cut(s) 57, 652, 922
BseRI GAGGAG 2 cut(s) 849, 1161
BseXI GCAGC 7 cut(s) 84, 87, 350, 520, 523, 526, 1116
BseYI CCCAGC 1 cut(s) 529
Bsh1236I CGCG 1 cut(s) 141
BshFI GGCC 4 cut(s) 429, 795, 924, 1195
BslFI GGGAC 1 cut(s) 429
BslI CCNNNNNNNGG 1 cut(s) 186
BsmAI GTCTC 2 cut(s) 632, 689
BsmFI GGGAC 1 cut(s) 429
BsmI GAATGC 1 cut(s) 1132
BsnI GGCC 4 cut(s) 429, 795, 924, 1195
Bso31I GGTCTC 1 cut(s) 632
Bsp143I GATC 7 cut(s) 275, 310, 316, 397, 739, 1024, 1030
Bsp1720I GCTNAGC 1 cut(s) 88
Bsp19I CCATGG 1 cut(s) 334
BspACI CCGC 4 cut(s) 163, 223, 921, 1044
BspANI GGCC 4 cut(s) 429, 795, 924, 1195
BspCNI CTCAG 1 cut(s) 729
BspFNI CGCG 1 cut(s) 141
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 2 cut(s) 239, 884
BspMAI CTGCAG 1 cut(s) 262
BspPI GGATC 3 cut(s) 270, 392, 1025
BspQI GCTCTTC 1 cut(s) 825
BspTNI GGTCTC 1 cut(s) 632
BsrDI GCAATG 1 cut(s) 430
BsrI ACTGG 3 cut(s) 57, 652, 922
BssECI CCNNGG 1 cut(s) 334
BssMI GATC 7 cut(s) 275, 310, 316, 397, 739, 1024, 1030
BssT1I CCWWGG 1 cut(s) 334
Bst2UI CCWGG 1 cut(s) 797
Bst4CI ACNGT 1 cut(s) 1071
Bst6I CTCTTC 3 cut(s) 253, 437, 825
BstC8I GCNNGC 4 cut(s) 106, 143, 147, 192
BstDEI CTNAG 3 cut(s) 88, 716, 1061
BstDSI CCRYGG 1 cut(s) 334
BstF5I GGATG 3 cut(s) 897, 1118, 1175
BstFNI CGCG 1 cut(s) 141
BstHHI GCGC 2 cut(s) 141, 779
BstKTI GATC 7 cut(s) 278, 313, 319, 400, 742, 1027, 1033
BstMAI GTCTC 2 cut(s) 632, 689
BstMBI GATC 7 cut(s) 275, 310, 316, 397, 739, 1024, 1030
BstMWI GCNNNNNNNGC 3 cut(s) 72, 84, 539
BstNI CCWGG 1 cut(s) 797
BstSCI CCNGG 1 cut(s) 795
BstSFI CTRYAG 1 cut(s) 258
BstUI CGCG 1 cut(s) 141
BstV1I GCAGC 7 cut(s) 84, 87, 350, 520, 523, 526, 1116
BstX2I RGATCY 1 cut(s) 275
BstXI CCANNNNNNTGG 1 cut(s) 578
BstYI RGATCY 1 cut(s) 275
BsuRI GGCC 4 cut(s) 429, 795, 924, 1195
BtgI CCRYGG 1 cut(s) 334
BtsCI GGATG 3 cut(s) 897, 1118, 1175
Cac8I GCNNGC 4 cut(s) 106, 143, 147, 192
CaiI CAGNNNCTG 1 cut(s) 536
CciI TCATGA 1 cut(s) 313
CfoI GCGC 2 cut(s) 141, 779
Cfr13I GGNCC 1 cut(s) 160
CseI GACGC 1 cut(s) 523
Csp6I GTAC 2 cut(s) 462, 1015
CviAII CATG 5 cut(s) 314, 335, 395, 1019, 1191
CviQI GTAC 2 cut(s) 462, 1015
DdeI CTNAG 3 cut(s) 88, 716, 1061
DpnI GATC 7 cut(s) 277, 312, 318, 399, 741, 1026, 1032
DpnII GATC 7 cut(s) 275, 310, 316, 397, 739, 1024, 1030
EaeI YGGCCR 2 cut(s) 922, 1193
Eam1104I CTCTTC 3 cut(s) 253, 437, 825
EarI CTCTTC 3 cut(s) 253, 437, 825
Eco130I CCWWGG 1 cut(s) 334
Eco147I AGGCCT 1 cut(s) 795
Eco31I GGTCTC 1 cut(s) 632
Eco47I GGWCC 1 cut(s) 160
EcoRII CCWGG 1 cut(s) 795
EcoT14I CCWWGG 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 334
FaeI CATG 5 cut(s) 317, 338, 398, 1022, 1194
FaqI GGGAC 1 cut(s) 429
FatI CATG 5 cut(s) 313, 334, 394, 1018, 1190
FbaI TGATCA 1 cut(s) 310
FblI GTMKAC 1 cut(s) 914
Fnu4HI GCNGC 8 cut(s) 73, 76, 364, 534, 537, 540, 922, 1130
FokI GGATG 3 cut(s) 904, 1105, 1162
Fsp4HI GCNGC 8 cut(s) 73, 76, 364, 534, 537, 540, 922, 1130
FspBI CTAG 2 cut(s) 69, 465
GlaI GCGC 2 cut(s) 140, 778
GluI GCNGC 8 cut(s) 73, 76, 364, 534, 537, 540, 922, 1130
GsaI CCCAGC 1 cut(s) 533
HaeIII GGCC 4 cut(s) 429, 795, 924, 1195
HgaI GACGC 1 cut(s) 523
HhaI GCGC 2 cut(s) 141, 779
Hin1II CATG 5 cut(s) 317, 338, 398, 1022, 1194
Hin6I GCGC 2 cut(s) 139, 777
HinP1I GCGC 2 cut(s) 139, 777
HincII GTYRAC 1 cut(s) 557
HindII GTYRAC 1 cut(s) 557
HinfI GANTC 3 cut(s) 33, 697, 911
HphI GGTGA 3 cut(s) 212, 281, 603
Hpy166II GTNNAC 3 cut(s) 557, 915, 1067
Hpy188I TCNGA 4 cut(s) 205, 237, 694, 842
Hpy188III TCNNGA 7 cut(s) 308, 314, 406, 511, 1028, 1094, 1139
Hpy8I GTNNAC 3 cut(s) 557, 915, 1067
HpyAV CCTTC 3 cut(s) 242, 612, 1100
HpyCH4III ACNGT 1 cut(s) 1071
HpyCH4V TGCA 7 cut(s) 260, 366, 423, 931, 1041, 1132, 1190
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 84, 539
HpyF3I CTNAG 3 cut(s) 88, 716, 1061
Hsp92II CATG 5 cut(s) 317, 338, 398, 1022, 1194
HspAI GCGC 2 cut(s) 139, 777
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 7 cut(s) 275, 310, 316, 397, 739, 1024, 1030
LguI GCTCTTC 1 cut(s) 825
Lsp1109I GCAGC 7 cut(s) 84, 87, 350, 520, 523, 526, 1116
LweI GCATC 1 cut(s) 940
MaeI CTAG 2 cut(s) 69, 465
MaeIII GTNAC 5 cut(s) 218, 269, 329, 866, 1165
MalI GATC 7 cut(s) 277, 312, 318, 399, 741, 1026, 1032
MboI GATC 7 cut(s) 275, 310, 316, 397, 739, 1024, 1030
MboII GAAGA 4 cut(s) 240, 424, 842, 886
MflI RGATCY 1 cut(s) 275
MlsI TGGCCA 1 cut(s) 1195
MluCI AATT 6 cut(s) 15, 212, 376, 522, 780, 965
MluNI TGGCCA 1 cut(s) 1195
MlyI GAGTC 2 cut(s) 691, 920
MmeI TCCRAC 2 cut(s) 686, 820
MnlI CCTC 9 cut(s) 106, 381, 440, 624, 702, 867, 870, 1153, 1182
Mox20I TGGCCA 1 cut(s) 1195
MscI TGGCCA 1 cut(s) 1195
MseI TTAA 4 cut(s) 18, 375, 489, 906
Msp20I TGGCCA 1 cut(s) 1195
MspA1I CMGCKG 1 cut(s) 533
MspR9I CCNGG 1 cut(s) 797
Mva1269I GAATGC 1 cut(s) 1132
MvaI CCWGG 1 cut(s) 797
MvnI CGCG 1 cut(s) 141
MwoI GCNNNNNNNGC 3 cut(s) 72, 84, 539
NcoI CCATGG 1 cut(s) 334
NdeII GATC 7 cut(s) 275, 310, 316, 397, 739, 1024, 1030
NlaIII CATG 5 cut(s) 317, 338, 398, 1022, 1194
NlaIV GGNNCC 2 cut(s) 239, 884
NmuCI GTSAC 2 cut(s) 218, 269
PagI TCATGA 1 cut(s) 313
PceI AGGCCT 1 cut(s) 795
PciSI GCTCTTC 1 cut(s) 825
PctI GAATGC 1 cut(s) 1132
PfeI GAWTC 1 cut(s) 33
PflMI CCANNNNNTGG 1 cut(s) 186
PkrI GCNGC 8 cut(s) 74, 77, 365, 535, 538, 541, 923, 1131
PleI GAGTC 2 cut(s) 691, 919
PpsI GAGTC 2 cut(s) 691, 919
PshBI ATTAAT 1 cut(s) 18
PsiI TTATAA 1 cut(s) 731
Psp6I CCWGG 1 cut(s) 795
PspFI CCCAGC 1 cut(s) 529
PspGI CCWGG 1 cut(s) 795
PspN4I GGNNCC 2 cut(s) 239, 884
PspPI GGNCC 1 cut(s) 160
PstI CTGCAG 1 cut(s) 262
PstNI CAGNNNCTG 1 cut(s) 536
PsuI RGATCY 1 cut(s) 275
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 2 cut(s) 463, 1016
RsaNI GTAC 2 cut(s) 462, 1015
SapI GCTCTTC 1 cut(s) 825
SaqAI TTAA 4 cut(s) 18, 375, 489, 906
SatI GCNGC 8 cut(s) 73, 76, 364, 534, 537, 540, 922, 1130
Sau3AI GATC 7 cut(s) 275, 310, 316, 397, 739, 1024, 1030
Sau96I GGNCC 1 cut(s) 160
ScaI AGTACT 1 cut(s) 463
SchI GAGTC 2 cut(s) 691, 920
ScrFI CCNGG 1 cut(s) 797
SetI ASST 8 cut(s) 89, 151, 535, 616, 627, 837, 1164, 1206
SfaNI GCATC 1 cut(s) 940
SfcI CTRYAG 1 cut(s) 258
SinI GGWCC 1 cut(s) 160
SmlI CTYRAG 2 cut(s) 635, 1092
SmoI CTYRAG 2 cut(s) 635, 1092
SpeI ACTAGT 1 cut(s) 464
Sse9I AATT 6 cut(s) 15, 212, 376, 522, 780, 965
SseBI AGGCCT 1 cut(s) 795
SsiI CCGC 4 cut(s) 163, 223, 921, 1044
SspI AATATT 1 cut(s) 659
SspMI CTAG 2 cut(s) 69, 465
StuI AGGCCT 1 cut(s) 795
StyD4I CCNGG 1 cut(s) 795
StyI CCWWGG 1 cut(s) 334
TaaI ACNGT 1 cut(s) 1071
TaqI TCGA 3 cut(s) 8, 151, 405
TasI AATT 6 cut(s) 15, 212, 376, 522, 780, 965
TatI WGTACW 1 cut(s) 461
TauI GCSGC 1 cut(s) 924
TfiI GAWTC 1 cut(s) 33
Tru1I TTAA 4 cut(s) 18, 375, 489, 906
Tru9I TTAA 4 cut(s) 18, 375, 489, 906
TseFI GTSAC 2 cut(s) 218, 269
TseI GCWGC 7 cut(s) 72, 75, 363, 533, 536, 539, 1129
Tsp45I GTSAC 2 cut(s) 218, 269
TspDTI ATGAA 8 cut(s) 17, 678, 768, 773, 843, 1107, 1113, 1119
TspGWI ACGGA 1 cut(s) 125
Van91I CCANNNNNTGG 1 cut(s) 186
VpaK11BI GGWCC 1 cut(s) 160
VspI ATTAAT 1 cut(s) 18
XapI RAATTY 2 cut(s) 212, 965
XcmI CCANNNNNNNNNTGG 1 cut(s) 422
XmiI GTMKAC 1 cut(s) 914
XspI CTAG 2 cut(s) 69, 465
ZrmI AGTACT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.