Belongs to the nucleosome assembly protein (NAP) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 3 cut(s) | 272, 836, 878 |
| AciI | CCGC | 2 cut(s) | 160, 746 |
| AclWI | GGATC | 2 cut(s) | 69, 688 |
| AcoI | YGGCCR | 1 cut(s) | 108 |
| AcuI | CTGAAG | 2 cut(s) | 333, 381 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 160, 677 |
| AflII | CTTAAG | 1 cut(s) | 659 |
| AflIII | ACRYGT | 1 cut(s) | 27 |
| AgsI | TTSAA | 5 cut(s) | 239, 308, 505, 623, 733 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 832 |
| AjiI | CACGTC | 1 cut(s) | 58 |
| AluBI | AGCT | 6 cut(s) | 47, 138, 233, 311, 419, 655 |
| AluI | AGCT | 6 cut(s) | 47, 138, 233, 311, 419, 655 |
| Alw21I | GWGCWC | 1 cut(s) | 1063 |
| Alw26I | GTCTC | 2 cut(s) | 459, 906 |
| AlwI | GGATC | 2 cut(s) | 69, 688 |
| Ama87I | CYCGRG | 2 cut(s) | 131, 596 |
| AoxI | GGCC | 4 cut(s) | 70, 108, 888, 1085 |
| ApeKI | GCWGC | 3 cut(s) | 6, 115, 230 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 591 |
| AsuC2I | CCSGG | 2 cut(s) | 112, 1069 |
| AsuHPI | GGTGA | 1 cut(s) | 1091 |
| AsuNHI | GCTAGC | 2 cut(s) | 415, 1037 |
| AvaI | CYCGRG | 2 cut(s) | 131, 596 |
| AxyI | CCTNAGG | 2 cut(s) | 495, 759 |
| BanII | GRGCYC | 1 cut(s) | 451 |
| BbsI | GAAGAC | 3 cut(s) | 984, 1002, 1034 |
| Bbv12I | GWGCWC | 1 cut(s) | 1063 |
| BbvCI | CCTCAGC | 2 cut(s) | 43, 420 |
| BbvI | GCAGC | 3 cut(s) | 18, 127, 242 |
| BccI | CCATC | 1 cut(s) | 805 |
| BciVI | GTATCC | 1 cut(s) | 639 |
| BcnI | CCSGG | 2 cut(s) | 112, 1069 |
| BcoDI | GTCTC | 2 cut(s) | 459, 906 |
| BfaI | CTAG | 3 cut(s) | 416, 705, 1038 |
| BfmI | CTRYAG | 1 cut(s) | 147 |
| BfrI | CTTAAG | 1 cut(s) | 659 |
| BfuI | GTATCC | 1 cut(s) | 639 |
| BisI | GCNGC | 3 cut(s) | 7, 116, 231 |
| BlpI | GCTNAGC | 1 cut(s) | 693 |
| BlsI | GCNGC | 3 cut(s) | 8, 117, 232 |
| Bme1390I | CCNGG | 2 cut(s) | 112, 1069 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 832 |
| BmeT110I | CYCGRG | 2 cut(s) | 131, 596 |
| BmgBI | CACGTC | 1 cut(s) | 58 |
| BmrFI | CCNGG | 2 cut(s) | 112, 1069 |
| BmtI | GCTAGC | 2 cut(s) | 419, 1041 |
| BpiI | GAAGAC | 3 cut(s) | 984, 1002, 1034 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 57, 89, 127, 159, 217, 249 |
| BpmI | CTGGAG | 3 cut(s) | 530, 903, 1074 |
| Bpu10I | CCTNAGC | 2 cut(s) | 43, 420 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 693 |
| BpuEI | CTTGAG | 3 cut(s) | 71, 224, 735 |
| BpuMI | CCSGG | 2 cut(s) | 112, 1069 |
| BsaJI | CCNNGG | 2 cut(s) | 792, 1068 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 639, 669 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 160, 677 |
| Bse1I | ACTGG | 2 cut(s) | 513, 886 |
| Bse21I | CCTNAGG | 2 cut(s) | 495, 759 |
| Bse3DI | GCAATG | 1 cut(s) | 402 |
| BseDI | CCNNGG | 2 cut(s) | 792, 1068 |
| BseGI | GGATG | 2 cut(s) | 790, 976 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 160, 677 |
| BseMI | GCAATG | 1 cut(s) | 402 |
| BseMII | CTCAG | 4 cut(s) | 57, 411, 423, 750 |
| BseNI | ACTGG | 2 cut(s) | 513, 886 |
| BseRI | GAGGAG | 3 cut(s) | 31, 236, 437 |
| BseXI | GCAGC | 3 cut(s) | 18, 127, 242 |
| BshFI | GGCC | 4 cut(s) | 72, 110, 890, 1087 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1063 |
| BsiHKCI | CYCGRG | 2 cut(s) | 131, 596 |
| BsiSI | CCGG | 2 cut(s) | 111, 1068 |
| BslFI | GGGAC | 2 cut(s) | 625, 740 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 160, 677 |
| BsmAI | GTCTC | 2 cut(s) | 459, 906 |
| BsmFI | GGGAC | 2 cut(s) | 625, 740 |
| BsmI | GAATGC | 2 cut(s) | 694, 1100 |
| BsnI | GGCC | 4 cut(s) | 72, 110, 890, 1087 |
| BsoBI | CYCGRG | 2 cut(s) | 131, 596 |
| Bsp1286I | GDGCHC | 2 cut(s) | 451, 1063 |
| Bsp143I | GATC | 4 cut(s) | 34, 61, 680, 852 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 693 |
| BspACI | CCGC | 2 cut(s) | 160, 746 |
| BspANI | GGCC | 4 cut(s) | 72, 110, 890, 1087 |
| BspCNI | CTCAG | 4 cut(s) | 56, 412, 424, 751 |
| BspMAI | CTGCAG | 1 cut(s) | 151 |
| BspOI | GCTAGC | 2 cut(s) | 419, 1041 |
| BspPI | GGATC | 2 cut(s) | 69, 688 |
| BspTI | CTTAAG | 1 cut(s) | 659 |
| BsrDI | GCAATG | 1 cut(s) | 402 |
| BsrI | ACTGG | 2 cut(s) | 513, 886 |
| BssECI | CCNNGG | 2 cut(s) | 792, 1068 |
| BssMI | GATC | 4 cut(s) | 34, 61, 680, 852 |
| BssNAI | GTATAC | 2 cut(s) | 273, 879 |
| Bst1107I | GTATAC | 2 cut(s) | 273, 879 |
| Bst6I | CTCTTC | 3 cut(s) | 339, 942, 1043 |
| BstAFI | CTTAAG | 1 cut(s) | 659 |
| BstC8I | GCNNGC | 3 cut(s) | 70, 417, 1039 |
| BstDEI | CTNAG | 9 cut(s) | 43, 173, 420, 432, 461, 495, 672, 693, 759 |
| BstF5I | GGATG | 2 cut(s) | 790, 976 |
| BstKTI | GATC | 4 cut(s) | 37, 64, 683, 855 |
| BstMAI | GTCTC | 2 cut(s) | 459, 906 |
| BstMBI | GATC | 4 cut(s) | 34, 61, 680, 852 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 239 |
| BstNSI | RCATGY | 1 cut(s) | 31 |
| BstSCI | CCNGG | 2 cut(s) | 110, 1067 |
| BstSFI | CTRYAG | 1 cut(s) | 147 |
| BstV1I | GCAGC | 3 cut(s) | 18, 127, 242 |
| BstV2I | GAAGAC | 3 cut(s) | 984, 1002, 1034 |
| BstZ17I | GTATAC | 2 cut(s) | 273, 879 |
| Bsu36I | CCTNAGG | 2 cut(s) | 495, 759 |
| BsuI | GTATCC | 1 cut(s) | 639 |
| BsuRI | GGCC | 4 cut(s) | 72, 110, 890, 1087 |
| BtrI | CACGTC | 1 cut(s) | 58 |
| BtsCI | GGATG | 2 cut(s) | 790, 976 |
| Cac8I | GCNNGC | 3 cut(s) | 70, 417, 1039 |
| CviAII | CATG | 5 cut(s) | 28, 194, 571, 863, 873 |
| DdeI | CTNAG | 9 cut(s) | 43, 173, 420, 432, 461, 495, 672, 693, 759 |
| DpnI | GATC | 4 cut(s) | 36, 63, 682, 854 |
| DpnII | GATC | 4 cut(s) | 34, 61, 680, 852 |
| DriI | GACNNNNNGTC | 1 cut(s) | 832 |
| EaeI | YGGCCR | 1 cut(s) | 108 |
| Eam1104I | CTCTTC | 3 cut(s) | 339, 942, 1043 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 832 |
| EarI | CTCTTC | 3 cut(s) | 339, 942, 1043 |
| EciI | GGCGGA | 1 cut(s) | 735 |
| Eco147I | AGGCCT | 2 cut(s) | 72, 1087 |
| Eco24I | GRGCYC | 1 cut(s) | 451 |
| Eco32I | GATATC | 2 cut(s) | 127, 429 |
| Eco57I | CTGAAG | 2 cut(s) | 333, 381 |
| Eco81I | CCTNAGG | 2 cut(s) | 495, 759 |
| Eco88I | CYCGRG | 2 cut(s) | 131, 596 |
| EcoRV | GATATC | 2 cut(s) | 127, 429 |
| EcoT38I | GRGCYC | 1 cut(s) | 451 |
| FaeI | CATG | 5 cut(s) | 31, 197, 574, 866, 876 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 435, 467 |
| FaqI | GGGAC | 2 cut(s) | 625, 740 |
| FatI | CATG | 5 cut(s) | 27, 193, 570, 862, 872 |
| FauI | CCCGC | 1 cut(s) | 153 |
| FblI | GTMKAC | 3 cut(s) | 272, 836, 878 |
| Fnu4HI | GCNGC | 3 cut(s) | 7, 116, 231 |
| FokI | GGATG | 2 cut(s) | 797, 983 |
| FriOI | GRGCYC | 1 cut(s) | 451 |
| Fsp4HI | GCNGC | 3 cut(s) | 7, 116, 231 |
| FspBI | CTAG | 3 cut(s) | 416, 705, 1038 |
| GluI | GCNGC | 3 cut(s) | 7, 116, 231 |
| GsuI | CTGGAG | 3 cut(s) | 530, 903, 1074 |
| HaeIII | GGCC | 4 cut(s) | 72, 110, 890, 1087 |
| HapII | CCGG | 2 cut(s) | 111, 1068 |
| Hin1II | CATG | 5 cut(s) | 31, 197, 574, 866, 876 |
| HinfI | GANTC | 3 cut(s) | 181, 318, 356 |
| HpaII | CCGG | 2 cut(s) | 111, 1068 |
| HphI | GGTGA | 1 cut(s) | 1091 |
| Hpy166II | GTNNAC | 3 cut(s) | 273, 837, 879 |
| Hpy188I | TCNGA | 7 cut(s) | 34, 124, 155, 186, 252, 361, 433 |
| Hpy188III | TCNNGA | 8 cut(s) | 88, 133, 236, 553, 596, 598, 845, 1091 |
| Hpy8I | GTNNAC | 3 cut(s) | 273, 837, 879 |
| Hpy99I | CGWCG | 2 cut(s) | 59, 770 |
| HpyAV | CCTTC | 5 cut(s) | 308, 437, 642, 670, 1004 |
| HpyCH4IV | ACGT | 1 cut(s) | 57 |
| HpyCH4V | TGCA | 3 cut(s) | 149, 1055, 1098 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 239 |
| HpyF3I | CTNAG | 9 cut(s) | 43, 173, 420, 432, 461, 495, 672, 693, 759 |
| HpySE526I | ACGT | 1 cut(s) | 57 |
| Hsp92II | CATG | 5 cut(s) | 31, 197, 574, 866, 876 |
| Kzo9I | GATC | 4 cut(s) | 34, 61, 680, 852 |
| LmnI | GCTCC | 2 cut(s) | 660, 1072 |
| Lsp1109I | GCAGC | 3 cut(s) | 18, 127, 242 |
| MaeI | CTAG | 3 cut(s) | 416, 705, 1038 |
| MaeII | ACGT | 1 cut(s) | 57 |
| MaeIII | GTNAC | 2 cut(s) | 319, 334 |
| MalI | GATC | 4 cut(s) | 36, 63, 682, 854 |
| MboI | GATC | 4 cut(s) | 34, 61, 680, 852 |
| MhlI | GDGCHC | 2 cut(s) | 451, 1063 |
| MluCI | AATT | 3 cut(s) | 254, 295, 715 |
| MlyI | GAGTC | 1 cut(s) | 327 |
| MroXI | GAANNNNTTC | 1 cut(s) | 591 |
| MseI | TTAA | 3 cut(s) | 453, 540, 660 |
| MslI | CAYNNNNRTG | 1 cut(s) | 26 |
| MspCI | CTTAAG | 1 cut(s) | 659 |
| MspI | CCGG | 2 cut(s) | 111, 1068 |
| MspR9I | CCNGG | 2 cut(s) | 112, 1069 |
| Mva1269I | GAATGC | 2 cut(s) | 694, 1100 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 239 |
| NciI | CCSGG | 2 cut(s) | 112, 1069 |
| NdeII | GATC | 4 cut(s) | 34, 61, 680, 852 |
| NheI | GCTAGC | 2 cut(s) | 415, 1037 |
| NlaIII | CATG | 5 cut(s) | 31, 197, 574, 866, 876 |
| NmeAIII | GCCGAG | 1 cut(s) | 817 |
| NmuCI | GTSAC | 1 cut(s) | 319 |
| NspI | RCATGY | 1 cut(s) | 31 |
| PaeR7I | CTCGAG | 2 cut(s) | 131, 596 |
| PceI | AGGCCT | 2 cut(s) | 72, 1087 |
| PciI | ACATGT | 1 cut(s) | 27 |
| PctI | GAATGC | 2 cut(s) | 694, 1100 |
| PdmI | GAANNNNTTC | 1 cut(s) | 591 |
| PfeI | GAWTC | 2 cut(s) | 181, 356 |
| PkrI | GCNGC | 3 cut(s) | 8, 117, 232 |
| PleI | GAGTC | 1 cut(s) | 326 |
| PpsI | GAGTC | 1 cut(s) | 326 |
| PscI | ACATGT | 1 cut(s) | 27 |
| PstI | CTGCAG | 1 cut(s) | 151 |
| RseI | CAYNNNNRTG | 1 cut(s) | 26 |
| SaqAI | TTAA | 3 cut(s) | 453, 540, 660 |
| SatI | GCNGC | 3 cut(s) | 7, 116, 231 |
| Sau3AI | GATC | 4 cut(s) | 34, 61, 680, 852 |
| SchI | GAGTC | 1 cut(s) | 327 |
| ScrFI | CCNGG | 2 cut(s) | 112, 1069 |
| SduI | GDGCHC | 2 cut(s) | 451, 1063 |
| SetI | ASST | 8 cut(s) | 49, 60, 140, 235, 268, 313, 421, 657 |
| SfcI | CTRYAG | 1 cut(s) | 147 |
| Sfr274I | CTCGAG | 2 cut(s) | 131, 596 |
| SlaI | CTCGAG | 2 cut(s) | 131, 596 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 26 |
| SmlI | CTYRAG | 6 cut(s) | 86, 131, 203, 596, 659, 750 |
| SmoI | CTYRAG | 6 cut(s) | 86, 131, 203, 596, 659, 750 |
| Sse9I | AATT | 3 cut(s) | 254, 295, 715 |
| SseBI | AGGCCT | 2 cut(s) | 72, 1087 |
| SsiI | CCGC | 2 cut(s) | 160, 746 |
| SspMI | CTAG | 3 cut(s) | 416, 705, 1038 |
| StuI | AGGCCT | 2 cut(s) | 72, 1087 |
| StyD4I | CCNGG | 2 cut(s) | 110, 1067 |
| TaiI | ACGT | 1 cut(s) | 60 |
| TaqI | TCGA | 7 cut(s) | 64, 132, 165, 219, 597, 683, 844 |
| TasI | AATT | 3 cut(s) | 254, 295, 715 |
| TfiI | GAWTC | 2 cut(s) | 181, 356 |
| Tru1I | TTAA | 3 cut(s) | 453, 540, 660 |
| Tru9I | TTAA | 3 cut(s) | 453, 540, 660 |
| TseFI | GTSAC | 1 cut(s) | 319 |
| TseI | GCWGC | 3 cut(s) | 6, 115, 230 |
| Tsp45I | GTSAC | 1 cut(s) | 319 |
| TspGWI | ACGGA | 1 cut(s) | 629 |
| Vha464I | CTTAAG | 1 cut(s) | 659 |
| XceI | RCATGY | 1 cut(s) | 31 |
| XhoI | CTCGAG | 2 cut(s) | 131, 596 |
| XmiI | GTMKAC | 3 cut(s) | 272, 836, 878 |
| XmnI | GAANNNNTTC | 1 cut(s) | 591 |
| XspI | CTAG | 3 cut(s) | 416, 705, 1038 |