Belongs to the nucleosome assembly protein (NAP) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 872 |
| AccB1I | GGYRCC | 2 cut(s) | 382, 413 |
| AciI | CCGC | 3 cut(s) | 66, 114, 525 |
| AclWI | GGATC | 3 cut(s) | 12, 31, 293 |
| AcoI | YGGCCR | 1 cut(s) | 255 |
| AcsI | RAATTY | 2 cut(s) | 336, 693 |
| AcuI | CTGAAG | 2 cut(s) | 217, 663 |
| AfaI | GTAC | 5 cut(s) | 191, 452, 491, 582, 662 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 562, 683, 1049 |
| AgeI | ACCGGT | 1 cut(s) | 1049 |
| AgsI | TTSAA | 5 cut(s) | 121, 346, 373, 445, 1024 |
| AjnI | CCWGG | 1 cut(s) | 592 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 636, 668 |
| AleI | CACNNNNGTG | 1 cut(s) | 128 |
| AluBI | AGCT | 6 cut(s) | 45, 237, 799, 998, 1082, 1105 |
| AluI | AGCT | 6 cut(s) | 45, 237, 799, 998, 1082, 1105 |
| Alw21I | GWGCWC | 2 cut(s) | 785, 1000 |
| Alw26I | GTCTC | 5 cut(s) | 36, 162, 248, 858, 880 |
| AlwI | GGATC | 3 cut(s) | 12, 31, 293 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 34, 1085 |
| Ama87I | CYCGRG | 1 cut(s) | 83 |
| AoxI | GGCC | 2 cut(s) | 135, 255 |
| ApeKI | GCWGC | 8 cut(s) | 209, 796, 1073, 1076, 1079, 1082, 1085, 1094 |
| ApoI | RAATTY | 2 cut(s) | 336, 693 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 154, 186 |
| AsiGI | ACCGGT | 1 cut(s) | 1049 |
| AspLEI | GCGC | 2 cut(s) | 113, 650 |
| AspS9I | GGNCC | 1 cut(s) | 470 |
| AsuHPI | GGTGA | 2 cut(s) | 848, 923 |
| AsuII | TTCGAA | 1 cut(s) | 173 |
| AvaI | CYCGRG | 1 cut(s) | 83 |
| AvaII | GGWCC | 1 cut(s) | 470 |
| BanI | GGYRCC | 2 cut(s) | 382, 413 |
| BanII | GRGCYC | 2 cut(s) | 84, 1000 |
| Bbv12I | GWGCWC | 2 cut(s) | 785, 1000 |
| BbvCI | CCTCAGC | 1 cut(s) | 528 |
| BbvI | GCAGC | 8 cut(s) | 196, 808, 1060, 1063, 1069, 1072, 1081, 1091 |
| BccI | CCATC | 3 cut(s) | 372, 611, 767 |
| BceAI | ACGGC | 2 cut(s) | 70, 242 |
| BciT130I | CCWGG | 1 cut(s) | 594 |
| BcoDI | GTCTC | 5 cut(s) | 36, 162, 248, 858, 880 |
| BfaI | CTAG | 1 cut(s) | 42 |
| BfmI | CTRYAG | 2 cut(s) | 219, 1077 |
| BfoI | RGCGCY | 1 cut(s) | 114 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 899 |
| BisI | GCNGC | 9 cut(s) | 114, 210, 797, 1074, 1077, 1080, 1083, 1086, 1095 |
| BlsI | GCNGC | 9 cut(s) | 115, 211, 798, 1075, 1078, 1081, 1084, 1087, 1096 |
| BmcAI | AGTACT | 1 cut(s) | 452 |
| Bme1390I | CCNGG | 1 cut(s) | 594 |
| Bme18I | GGWCC | 1 cut(s) | 470 |
| BmeT110I | CYCGRG | 1 cut(s) | 83 |
| BmgT120I | GGNCC | 1 cut(s) | 470 |
| BmiI | GGNNCC | 4 cut(s) | 58, 168, 384, 415 |
| BmrFI | CCNGG | 1 cut(s) | 594 |
| BmsI | GCATC | 5 cut(s) | 81, 530, 728, 904, 1006 |
| BplI | GAGNNNNNCTC | 3 cut(s) | 37, 176, 208 |
| Bpu10I | CCTNAGC | 1 cut(s) | 528 |
| Bpu14I | TTCGAA | 1 cut(s) | 173 |
| BsaI | GGTCTC | 1 cut(s) | 248 |
| BsaJI | CCNNGG | 4 cut(s) | 138, 394, 593, 952 |
| BsaWI | WCCGGW | 2 cut(s) | 992, 1049 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 44, 74, 1051, 1081 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 562, 683, 1049 |
| Bse118I | RCCGGY | 1 cut(s) | 1049 |
| BseBI | CCWGG | 1 cut(s) | 594 |
| BseDI | CCNNGG | 4 cut(s) | 138, 394, 593, 952 |
| BseGI | GGATG | 4 cut(s) | 383, 521, 719, 759 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 562, 683, 1049 |
| BseMII | CTCAG | 4 cut(s) | 519, 602, 1013, 1113 |
| BseRI | GAGGAG | 5 cut(s) | 49, 52, 255, 1053, 1056 |
| BseXI | GCAGC | 8 cut(s) | 196, 808, 1060, 1063, 1069, 1072, 1081, 1091 |
| BseYI | CCCAGC | 2 cut(s) | 107, 799 |
| BshFI | GGCC | 2 cut(s) | 137, 257 |
| BshNI | GGYRCC | 2 cut(s) | 382, 413 |
| BshTI | ACCGGT | 1 cut(s) | 1049 |
| BsiHKAI | GWGCWC | 2 cut(s) | 785, 1000 |
| BsiHKCI | CYCGRG | 1 cut(s) | 83 |
| BsiSI | CCGG | 3 cut(s) | 993, 1050, 1070 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 562, 683, 1049 |
| BsmAI | GTCTC | 5 cut(s) | 36, 162, 248, 858, 880 |
| BsmBI | CGTCTC | 1 cut(s) | 162 |
| BsmI | GAATGC | 1 cut(s) | 1019 |
| BsnI | GGCC | 2 cut(s) | 137, 257 |
| Bso31I | GGTCTC | 1 cut(s) | 248 |
| BsoBI | CYCGRG | 1 cut(s) | 83 |
| Bsp119I | TTCGAA | 1 cut(s) | 173 |
| Bsp1286I | GDGCHC | 3 cut(s) | 84, 785, 1000 |
| Bsp1407I | TGTACA | 1 cut(s) | 660 |
| Bsp143I | GATC | 5 cut(s) | 4, 36, 180, 298, 501 |
| BspACI | CCGC | 3 cut(s) | 66, 114, 525 |
| BspANI | GGCC | 2 cut(s) | 137, 257 |
| BspCNI | CTCAG | 4 cut(s) | 520, 601, 1012, 1112 |
| BspHI | TCATGA | 1 cut(s) | 975 |
| BspLI | GGNNCC | 4 cut(s) | 58, 168, 384, 415 |
| BspMAI | CTGCAG | 2 cut(s) | 223, 1081 |
| BspPI | GGATC | 3 cut(s) | 12, 31, 293 |
| BspT104I | TTCGAA | 1 cut(s) | 173 |
| BspT107I | GGYRCC | 2 cut(s) | 382, 413 |
| BspTNI | GGTCTC | 1 cut(s) | 248 |
| BsrFI | RCCGGY | 1 cut(s) | 1049 |
| BsrGI | TGTACA | 1 cut(s) | 660 |
| BssAI | RCCGGY | 1 cut(s) | 1049 |
| BssECI | CCNNGG | 4 cut(s) | 138, 394, 593, 952 |
| BssMI | GATC | 5 cut(s) | 4, 36, 180, 298, 501 |
| BssT1I | CCWWGG | 1 cut(s) | 394 |
| Bst2UI | CCWGG | 1 cut(s) | 594 |
| Bst4CI | ACNGT | 2 cut(s) | 404, 907 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 545, 1085 |
| BstAUI | TGTACA | 1 cut(s) | 660 |
| BstBI | TTCGAA | 1 cut(s) | 173 |
| BstC8I | GCNNGC | 5 cut(s) | 103, 198, 214, 418, 1107 |
| BstDEI | CTNAG | 4 cut(s) | 528, 588, 999, 1099 |
| BstDSI | CCRYGG | 2 cut(s) | 138, 952 |
| BstF5I | GGATG | 4 cut(s) | 383, 521, 719, 759 |
| BstH2I | RGCGCY | 1 cut(s) | 114 |
| BstHHI | GCGC | 2 cut(s) | 113, 650 |
| BstKTI | GATC | 5 cut(s) | 7, 39, 183, 301, 504 |
| BstMAI | GTCTC | 5 cut(s) | 36, 162, 248, 858, 880 |
| BstMBI | GATC | 5 cut(s) | 4, 36, 180, 298, 501 |
| BstNI | CCWGG | 1 cut(s) | 594 |
| BstSCI | CCNGG | 1 cut(s) | 592 |
| BstSFI | CTRYAG | 2 cut(s) | 219, 1077 |
| BstV1I | GCAGC | 8 cut(s) | 196, 808, 1060, 1063, 1069, 1072, 1081, 1091 |
| BstX2I | RGATCY | 1 cut(s) | 4 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 145 |
| BstYI | RGATCY | 1 cut(s) | 4 |
| BsuRI | GGCC | 2 cut(s) | 137, 257 |
| BtgI | CCRYGG | 2 cut(s) | 138, 952 |
| BtsCI | GGATG | 4 cut(s) | 383, 521, 719, 759 |
| BtsI | GCAGTG | 2 cut(s) | 216, 573 |
| BtsIMutI | CAGTG | 4 cut(s) | 216, 409, 573, 1007 |
| Cac8I | GCNNGC | 5 cut(s) | 103, 198, 214, 418, 1107 |
| CaiI | CAGNNNCTG | 2 cut(s) | 34, 1085 |
| CciI | TCATGA | 1 cut(s) | 975 |
| CfoI | GCGC | 2 cut(s) | 113, 650 |
| Cfr10I | RCCGGY | 1 cut(s) | 1049 |
| Cfr13I | GGNCC | 1 cut(s) | 470 |
| Csp6I | GTAC | 5 cut(s) | 190, 451, 490, 581, 661 |
| CspAI | ACCGGT | 1 cut(s) | 1049 |
| CviAII | CATG | 2 cut(s) | 253, 976 |
| CviQI | GTAC | 5 cut(s) | 190, 451, 490, 581, 661 |
| DdeI | CTNAG | 4 cut(s) | 528, 588, 999, 1099 |
| DpnI | GATC | 5 cut(s) | 6, 38, 182, 300, 503 |
| DpnII | GATC | 5 cut(s) | 4, 36, 180, 298, 501 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 872 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 872 |
| EaeI | YGGCCR | 1 cut(s) | 255 |
| EciI | GGCGGA | 1 cut(s) | 55 |
| Ecl136II | GAGCTC | 1 cut(s) | 998 |
| Eco130I | CCWWGG | 1 cut(s) | 394 |
| Eco24I | GRGCYC | 2 cut(s) | 84, 1000 |
| Eco31I | GGTCTC | 1 cut(s) | 248 |
| Eco47I | GGWCC | 1 cut(s) | 470 |
| Eco53kI | GAGCTC | 1 cut(s) | 998 |
| Eco57I | CTGAAG | 2 cut(s) | 217, 663 |
| Eco88I | CYCGRG | 1 cut(s) | 83 |
| EcoICRI | GAGCTC | 1 cut(s) | 998 |
| EcoRI | GAATTC | 1 cut(s) | 336 |
| EcoRII | CCWGG | 1 cut(s) | 592 |
| EcoT14I | CCWWGG | 1 cut(s) | 394 |
| EcoT22I | ATGCAT | 1 cut(s) | 1021 |
| EcoT38I | GRGCYC | 2 cut(s) | 84, 1000 |
| ErhI | CCWWGG | 1 cut(s) | 394 |
| Esp3I | CGTCTC | 1 cut(s) | 162 |
| FaeI | CATG | 2 cut(s) | 256, 979 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 307, 339, 636, 668 |
| FatI | CATG | 2 cut(s) | 252, 975 |
| Fnu4HI | GCNGC | 9 cut(s) | 114, 210, 797, 1074, 1077, 1080, 1083, 1086, 1095 |
| FokI | GGATG | 4 cut(s) | 390, 508, 706, 746 |
| FriOI | GRGCYC | 2 cut(s) | 84, 1000 |
| Fsp4HI | GCNGC | 9 cut(s) | 114, 210, 797, 1074, 1077, 1080, 1083, 1086, 1095 |
| FspBI | CTAG | 1 cut(s) | 42 |
| GlaI | GCGC | 2 cut(s) | 112, 649 |
| GluI | GCNGC | 9 cut(s) | 114, 210, 797, 1074, 1077, 1080, 1083, 1086, 1095 |
| GsaI | CCCAGC | 2 cut(s) | 111, 803 |
| HaeII | RGCGCY | 1 cut(s) | 114 |
| HaeIII | GGCC | 2 cut(s) | 137, 257 |
| HapII | CCGG | 3 cut(s) | 993, 1050, 1070 |
| HhaI | GCGC | 2 cut(s) | 113, 650 |
| Hin1II | CATG | 2 cut(s) | 256, 979 |
| Hin6I | GCGC | 2 cut(s) | 111, 648 |
| HinP1I | GCGC | 2 cut(s) | 111, 648 |
| HinfI | GANTC | 2 cut(s) | 228, 366 |
| HpaII | CCGG | 3 cut(s) | 993, 1050, 1070 |
| HphI | GGTGA | 2 cut(s) | 848, 923 |
| Hpy166II | GTNNAC | 2 cut(s) | 480, 948 |
| Hpy188I | TCNGA | 7 cut(s) | 36, 162, 460, 616, 643, 1102, 1118 |
| Hpy188III | TCNNGA | 4 cut(s) | 184, 269, 445, 976 |
| Hpy8I | GTNNAC | 2 cut(s) | 480, 948 |
| Hpy99I | CGWCG | 1 cut(s) | 166 |
| HpyAV | CCTTC | 5 cut(s) | 127, 180, 211, 565, 638 |
| HpyCH4III | ACNGT | 2 cut(s) | 404, 907 |
| HpyF3I | CTNAG | 4 cut(s) | 528, 588, 999, 1099 |
| Hsp92II | CATG | 2 cut(s) | 256, 979 |
| HspAI | GCGC | 2 cut(s) | 111, 648 |
| Kzo9I | GATC | 5 cut(s) | 4, 36, 180, 298, 501 |
| LmnI | GCTCC | 5 cut(s) | 62, 166, 349, 788, 995 |
| Lsp1109I | GCAGC | 8 cut(s) | 196, 808, 1060, 1063, 1069, 1072, 1081, 1091 |
| LweI | GCATC | 5 cut(s) | 81, 530, 728, 904, 1006 |
| MaeI | CTAG | 1 cut(s) | 42 |
| MaeIII | GTNAC | 3 cut(s) | 259, 398, 1057 |
| MalI | GATC | 5 cut(s) | 6, 38, 182, 300, 503 |
| MboI | GATC | 5 cut(s) | 4, 36, 180, 298, 501 |
| MboII | GAAGA | 7 cut(s) | 187, 190, 223, 315, 361, 934, 937 |
| MflI | RGATCY | 1 cut(s) | 4 |
| MhlI | GDGCHC | 3 cut(s) | 84, 785, 1000 |
| MluCI | AATT | 3 cut(s) | 336, 534, 693 |
| MmeI | TCCRAC | 2 cut(s) | 185, 1015 |
| Mph1103I | ATGCAT | 1 cut(s) | 1021 |
| MslI | CAYNNNNRTG | 3 cut(s) | 128, 251, 707 |
| MspA1I | CMGCKG | 4 cut(s) | 237, 527, 799, 1082 |
| MspI | CCGG | 3 cut(s) | 993, 1050, 1070 |
| MspR9I | CCNGG | 1 cut(s) | 594 |
| Mva1269I | GAATGC | 1 cut(s) | 1019 |
| MvaI | CCWGG | 1 cut(s) | 594 |
| NdeII | GATC | 5 cut(s) | 4, 36, 180, 298, 501 |
| NlaIII | CATG | 2 cut(s) | 256, 979 |
| NlaIV | GGNNCC | 4 cut(s) | 58, 168, 384, 415 |
| NmuCI | GTSAC | 2 cut(s) | 259, 1057 |
| NsiI | ATGCAT | 1 cut(s) | 1021 |
| NspV | TTCGAA | 1 cut(s) | 173 |
| OliI | CACNNNNGTG | 1 cut(s) | 128 |
| PaeR7I | CTCGAG | 1 cut(s) | 83 |
| PagI | TCATGA | 1 cut(s) | 975 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 170 |
| PctI | GAATGC | 1 cut(s) | 1019 |
| PfeI | GAWTC | 2 cut(s) | 228, 366 |
| PflFI | GACNNNGTC | 1 cut(s) | 275 |
| PinAI | ACCGGT | 1 cut(s) | 1049 |
| PkrI | GCNGC | 9 cut(s) | 115, 211, 798, 1075, 1078, 1081, 1084, 1087, 1096 |
| Psp124BI | GAGCTC | 1 cut(s) | 1000 |
| Psp6I | CCWGG | 1 cut(s) | 592 |
| PspFI | CCCAGC | 2 cut(s) | 107, 799 |
| PspGI | CCWGG | 1 cut(s) | 592 |
| PspN4I | GGNNCC | 4 cut(s) | 58, 168, 384, 415 |
| PspPI | GGNCC | 1 cut(s) | 470 |
| PspXI | VCTCGAGB | 1 cut(s) | 83 |
| PstI | CTGCAG | 2 cut(s) | 223, 1081 |
| PstNI | CAGNNNCTG | 2 cut(s) | 34, 1085 |
| PsuI | RGATCY | 1 cut(s) | 4 |
| PsyI | GACNNNGTC | 1 cut(s) | 275 |
| PvuII | CAGCTG | 3 cut(s) | 237, 799, 1082 |
| RsaI | GTAC | 5 cut(s) | 191, 452, 491, 582, 662 |
| RsaNI | GTAC | 5 cut(s) | 190, 451, 490, 581, 661 |
| RseI | CAYNNNNRTG | 3 cut(s) | 128, 251, 707 |
| SacI | GAGCTC | 1 cut(s) | 1000 |
| SatI | GCNGC | 9 cut(s) | 114, 210, 797, 1074, 1077, 1080, 1083, 1086, 1095 |
| Sau3AI | GATC | 5 cut(s) | 4, 36, 180, 298, 501 |
| Sau96I | GGNCC | 1 cut(s) | 470 |
| ScaI | AGTACT | 1 cut(s) | 452 |
| ScrFI | CCNGG | 1 cut(s) | 594 |
| SduI | GDGCHC | 3 cut(s) | 84, 785, 1000 |
| SfaNI | GCATC | 5 cut(s) | 81, 530, 728, 904, 1006 |
| SfcI | CTRYAG | 2 cut(s) | 219, 1077 |
| Sfr274I | CTCGAG | 1 cut(s) | 83 |
| SfuI | TTCGAA | 1 cut(s) | 173 |
| SgrAI | CRCCGGYG | 1 cut(s) | 1049 |
| SinI | GGWCC | 1 cut(s) | 470 |
| SlaI | CTCGAG | 1 cut(s) | 83 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 128, 251, 707 |
| SmlI | CTYRAG | 1 cut(s) | 83 |
| SmoI | CTYRAG | 1 cut(s) | 83 |
| Sse9I | AATT | 3 cut(s) | 336, 534, 693 |
| SsiI | CCGC | 3 cut(s) | 66, 114, 525 |
| SspMI | CTAG | 1 cut(s) | 42 |
| SstI | GAGCTC | 1 cut(s) | 1000 |
| StyD4I | CCNGG | 1 cut(s) | 592 |
| StyI | CCWWGG | 1 cut(s) | 394 |
| TaaI | ACNGT | 2 cut(s) | 404, 907 |
| TaqI | TCGA | 3 cut(s) | 84, 173, 183 |
| TasI | AATT | 3 cut(s) | 336, 534, 693 |
| TatI | WGTACW | 4 cut(s) | 189, 450, 489, 660 |
| TauI | GCSGC | 1 cut(s) | 116 |
| TfiI | GAWTC | 2 cut(s) | 228, 366 |
| TscAI | CASTG | 4 cut(s) | 223, 409, 573, 1007 |
| TseFI | GTSAC | 2 cut(s) | 259, 1057 |
| TseI | GCWGC | 8 cut(s) | 209, 796, 1073, 1076, 1079, 1082, 1085, 1094 |
| Tsp45I | GTSAC | 2 cut(s) | 259, 1057 |
| TspDTI | ATGAA | 1 cut(s) | 725 |
| TspGWI | ACGGA | 3 cut(s) | 144, 179, 956 |
| TspRI | CASTG | 4 cut(s) | 223, 409, 573, 1007 |
| Tth111I | GACNNNGTC | 1 cut(s) | 275 |
| VpaK11BI | GGWCC | 1 cut(s) | 470 |
| XapI | RAATTY | 2 cut(s) | 336, 693 |
| XhoI | CTCGAG | 1 cut(s) | 83 |
| XspI | CTAG | 1 cut(s) | 42 |
| ZrmI | AGTACT | 1 cut(s) | 452 |
| Zsp2I | ATGCAT | 1 cut(s) | 1021 |