Rh5CG459300

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000381
Physical Location & Seq
Forward (+)
63932764 .. 63934695
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG459300.1

Sequence Viewer

Length: 816 bp
ATGAGCAAAGACTTGAATAGCATCAATTTAACTGGCCAGCACACCATGGACATCCTTGAGAGCCTACCTCATAATGTCAGAAAGCGGGTTGAGTTCATGAGAGAAATTGAGAGTCAACGTGATGAATTAGAGGCCAAGCTCTTCAAGGAGAAAGCTGAACTTGAAGCCAAGTATCAGAAATTATATCAGAATCTCTATAGCAAGCGGTACTTGATTGTGAATGGTGTTGTTGAAACTGAAGGAGTTGCCACTGAAACAATAACAGATCATGAGAACAAAACTGCAAAAGAGAGAGGAGTGCCTGATTTCTGGCTGAATGCATTGAAGAATAATGCAGTGCTAGCCAAGGAGATTACCGAGTGTGATGAAGGAGCTCTTAAATATCTTAGGGACGTCAAGTGTTTTAAGATACGTAATTCGAAGGGGTTCAAGCTTGAGTTCGACTTTGACACCAATCCATTTTTCAAAAACTCTGTTTTGACAAAGACATATCACATGATTGATGAAGATGAACCAAACAGCACAGCAGGGACGGAGATTGAATGGTATCCAGCAAAATGCCTGACACAGAAGATTGTTAAGAAGAAGCATAAGAAGGGATCAAAGAATTCTAAGCTTCTGACTAAAACTAAAAATTGTAAAAGTTTCTTCAATTTCTTTAGCCCACATCAAGTCTCCAAGGACCATGAAGTTATTGATGAAGCTGCTGCCGAGGAACTCAAAAACCAGAAGAAACAAGACTATGATGTTGGGTCAACCATTTGTTATAAGATCATCCCACATGCAGTTTCGTGGTATACTGGAGAAGCTTTTTAG

Protein Analysis

271

Amino Acids

31.41

Weight (kDa)

8.1

Isoelectric Point (pI)

28.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAP PF00956 28 - 269 4.9e-74 Nucleosome assembly protein (NAP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000381)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19480 AT2G19480 AT2G19480 AT4G26110 AT4G26110 AT5G56950
fragaria_vesca FvH4_1g07740 FvH4_1g07740 FvH4_4g08240 FvH4_5g31310 FvH4_6g00310 FvH4_6g00310 FvH4_6g24290 FvH4_6g33640 FvH4_6g33640 FvH4_6g33640 FvH4_6g33640 FvH4_6g33640 FvH4_6g33640 FvH4_6g33640 FvH4_6g33650 FvH4_6g33710 FvH4_6g47360
malus_domestica MD02G1081800.v1.1 MD09G1192000.v1.1 MD15G1209100.v1.1
prunus_persica Prupe.3G048200_v2.0.a1 Prupe.3G048200_v2.0.a1 Prupe.7G207300_v2.0.a1 Prupe.7G207300_v2.0.a1 Prupe.7G207300_v2.0.a1
pyrus_communis pycom02g06460 pycom09g10850 pycom15g18540
rosa_chinensis RchiOBHm_Chr2g0093631 RchiOBHm_Chr2g0143531 RchiOBHm_Chr2g0143541 RchiOBHm_Chr2g0143561 RchiOBHm_Chr3g0453681 RchiOBHm_Chr3g0453701 RchiOBHm_Chr5g0064061
rosa_laevigata RLG00000015834 RLG00000016422 RLG00000020045 RLG00000020046 RLG00000035700
rosa_multiflora Rmu_co8360287.1_g000001 Rmu_sc0002126.1_g000019 Rmu_sc0003547.1_g000009 Rmu_sc0003556.1_g000007 Rmu_sc0003556.1_g000008 Rmu_sc0026989.1_g000002 Rmu_ssc0000231.1_g000007 Rmu_ssc0000239.1_g000004
rosa_roxburghii Rroxscaffold_1G00016760 Rroxscaffold_1G00016790 Rroxscaffold_2G00101660 Rroxscaffold_2G00101720 Rroxscaffold_2G00101740 Rroxscaffold_6G00430600 Rroxscaffold_6G00430620
rosa_rugosa Rorug02G0038100 Rorug02G0383200 Rorug02G0383300 Rorug02G0383400 Rorug02G0383500 Rorug02G0383800 Rorug02G0651100 Rorug02G0651200 Rorug05G0362500
rosa_samantha Rh1BG414000 Rh2AG084600 Rh2AG435400 Rh2AG435500 Rh2AG435700 Rh2AG588700 Rh2BG085200 Rh2BG443000 Rh2BG443100 Rh2BG443200 Rh2BG443300 Rh2CG087500 Rh2CG421500 Rh2CG421600 Rh2CG421800 Rh2DG083200 Rh2DG453500 Rh2DG453600 Rh2DG453700 Rh2DG611000 Rh5AG420500 Rh5AG421100 Rh5BG436400 Rh5CG459100 Rh5CG459300 Rh5DG449900
rosa_wichuraiana Rw2G007100 Rw2G035480 Rw2G035490 Rw2G035500 Rw3G004120 Rw3G004130 Rw5G039620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 768
AatII GACGTC 1 cut(s) 396
AccI GTMKAC 1 cut(s) 797
AciI CCGC 2 cut(s) 85, 205
AclWI GGATC 1 cut(s) 607
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 1 cut(s) 607
AcuI CTGAAG 1 cut(s) 258
AcyI GRCGYC 1 cut(s) 393
AfaI GTAC 1 cut(s) 209
AgsI TTSAA 9 cut(s) 16, 145, 164, 233, 325, 430, 466, 542, 652
AjuI GAANNNNNNNTTGG 2 cut(s) 446, 478
AluBI AGCT 7 cut(s) 139, 155, 374, 433, 616, 704, 809
AluI AGCT 7 cut(s) 139, 155, 374, 433, 616, 704, 809
Alw21I GWGCWC 1 cut(s) 376
Alw26I GTCTC 1 cut(s) 679
AlwI GGATC 1 cut(s) 607
AoxI GGCC 2 cut(s) 34, 132
ApeKI GCWGC 2 cut(s) 704, 707
ApoI RAATTY 1 cut(s) 607
Asp700I GAANNNNTTC 1 cut(s) 425
AspS9I GGNCC 1 cut(s) 682
AsuII TTCGAA 1 cut(s) 419
AsuNHI GCTAGC 1 cut(s) 340
AvaII GGWCC 1 cut(s) 682
BalI TGGCCA 1 cut(s) 36
BanII GRGCYC 1 cut(s) 376
Bbv12I GWGCWC 1 cut(s) 376
BbvI GCAGC 2 cut(s) 691, 694
BciVI GTATCC 1 cut(s) 558
BcoDI GTCTC 1 cut(s) 679
BfaI CTAG 1 cut(s) 341
BfmI CTRYAG 1 cut(s) 196
BfuI GTATCC 1 cut(s) 558
BisI GCNGC 2 cut(s) 705, 708
BlsI GCNGC 2 cut(s) 706, 709
Bme18I GGWCC 1 cut(s) 682
BmgT120I GGNCC 1 cut(s) 682
BmsI GCATC 1 cut(s) 30
BmtI GCTAGC 1 cut(s) 344
BplI GAGNNNNNCTC 2 cut(s) 52, 84
Bpu14I TTCGAA 1 cut(s) 419
BpuEI CTTGAG 2 cut(s) 77, 455
BsaAI YACGTR 1 cut(s) 413
BsaHI GRCGYC 1 cut(s) 393
BsaJI CCNNGG 4 cut(s) 45, 345, 678, 711
Bse1I ACTGG 2 cut(s) 37, 805
BseDI CCNNGG 4 cut(s) 45, 345, 678, 711
BseGI GGATG 2 cut(s) 51, 774
BseNI ACTGG 2 cut(s) 37, 805
BseRI GAGGAG 1 cut(s) 309
BseXI GCAGC 2 cut(s) 691, 694
BshFI GGCC 2 cut(s) 36, 134
BsiHKAI GWGCWC 1 cut(s) 376
BslFI GGGAC 2 cut(s) 404, 544
BsmAI GTCTC 1 cut(s) 679
BsmFI GGGAC 2 cut(s) 404, 544
BsmI GAATGC 1 cut(s) 322
BsnI GGCC 2 cut(s) 36, 134
Bsp119I TTCGAA 1 cut(s) 419
Bsp1286I GDGCHC 1 cut(s) 376
Bsp143I GATC 3 cut(s) 265, 599, 771
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 2 cut(s) 85, 205
BspANI GGCC 2 cut(s) 36, 134
BspHI TCATGA 2 cut(s) 96, 268
BspOI GCTAGC 1 cut(s) 344
BspPI GGATC 1 cut(s) 607
BspQI GCTCTTC 1 cut(s) 146
BspT104I TTCGAA 1 cut(s) 419
BsrI ACTGG 2 cut(s) 37, 805
BssECI CCNNGG 4 cut(s) 45, 345, 678, 711
BssMI GATC 3 cut(s) 265, 599, 771
BssNAI GTATAC 1 cut(s) 798
BssNI GRCGYC 1 cut(s) 393
BssT1I CCWWGG 3 cut(s) 45, 345, 678
Bst1107I GTATAC 1 cut(s) 798
Bst6I CTCTTC 1 cut(s) 146
BstACI GRCGYC 1 cut(s) 393
BstBAI YACGTR 1 cut(s) 413
BstBI TTCGAA 1 cut(s) 419
BstC8I GCNNGC 3 cut(s) 38, 203, 342
BstDEI CTNAG 2 cut(s) 386, 612
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 2 cut(s) 51, 774
BstKTI GATC 3 cut(s) 268, 602, 774
BstMAI GTCTC 1 cut(s) 679
BstMBI GATC 3 cut(s) 265, 599, 771
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstNSI RCATGY 1 cut(s) 785
BstSFI CTRYAG 1 cut(s) 196
BstSNI TACGTA 1 cut(s) 413
BstV1I GCAGC 2 cut(s) 691, 694
BstZ17I GTATAC 1 cut(s) 798
BsuI GTATCC 1 cut(s) 558
BsuRI GGCC 2 cut(s) 36, 134
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 2 cut(s) 51, 774
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 2 cut(s) 249, 342
Cac8I GCNNGC 3 cut(s) 38, 203, 342
CciI TCATGA 2 cut(s) 96, 268
Cfr13I GGNCC 1 cut(s) 682
Csp6I GTAC 1 cut(s) 208
CviAII CATG 6 cut(s) 46, 97, 269, 496, 686, 782
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 2 cut(s) 386, 612
DpnI GATC 3 cut(s) 267, 601, 773
DpnII GATC 3 cut(s) 265, 599, 771
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 1 cut(s) 146
EarI CTCTTC 1 cut(s) 146
Ecl136II GAGCTC 1 cut(s) 374
Eco105I TACGTA 1 cut(s) 413
Eco130I CCWWGG 3 cut(s) 45, 345, 678
Eco24I GRGCYC 1 cut(s) 376
Eco47I GGWCC 1 cut(s) 682
Eco53kI GAGCTC 1 cut(s) 374
Eco57I CTGAAG 1 cut(s) 258
EcoICRI GAGCTC 1 cut(s) 374
EcoRI GAATTC 1 cut(s) 607
EcoT14I CCWWGG 3 cut(s) 45, 345, 678
EcoT22I ATGCAT 1 cut(s) 322
EcoT38I GRGCYC 1 cut(s) 376
ErhI CCWWGG 3 cut(s) 45, 345, 678
FaeI CATG 6 cut(s) 49, 100, 272, 499, 689, 785
FalI AAGNNNNNCTT 6 cut(s) 144, 176, 194, 226, 360, 392
FaqI GGGAC 2 cut(s) 404, 544
FatI CATG 6 cut(s) 45, 96, 268, 495, 685, 781
FauI CCCGC 1 cut(s) 78
FblI GTMKAC 1 cut(s) 797
Fnu4HI GCNGC 2 cut(s) 705, 708
FokI GGATG 2 cut(s) 38, 761
FriOI GRGCYC 1 cut(s) 376
Fsp4HI GCNGC 2 cut(s) 705, 708
FspBI CTAG 1 cut(s) 341
GluI GCNGC 2 cut(s) 705, 708
HaeIII GGCC 2 cut(s) 36, 134
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 6 cut(s) 49, 100, 272, 499, 689, 785
HincII GTYRAC 2 cut(s) 116, 756
HindII GTYRAC 2 cut(s) 116, 756
HindIII AAGCTT 3 cut(s) 431, 614, 807
HinfI GANTC 2 cut(s) 112, 190
Hpy166II GTNNAC 3 cut(s) 116, 756, 798
Hpy188I TCNGA 4 cut(s) 80, 177, 189, 621
Hpy188III TCNNGA 2 cut(s) 97, 269
Hpy8I GTNNAC 3 cut(s) 116, 756, 798
HpyAV CCTTC 4 cut(s) 233, 362, 415, 589
HpyCH4IV ACGT 3 cut(s) 118, 393, 412
HpyCH4V TGCA 4 cut(s) 284, 320, 335, 785
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpyF3I CTNAG 2 cut(s) 386, 612
HpySE526I ACGT 3 cut(s) 118, 393, 412
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 6 cut(s) 49, 100, 272, 499, 689, 785
Kzo9I GATC 3 cut(s) 265, 599, 771
LguI GCTCTTC 1 cut(s) 146
LmnI GCTCC 1 cut(s) 371
LpnPI CCDG 9 cut(s) 18, 50, 295, 315, 513, 564, 575, 740, 786
Lsp1109I GCAGC 2 cut(s) 691, 694
LweI GCATC 1 cut(s) 30
MaeI CTAG 1 cut(s) 341
MaeII ACGT 3 cut(s) 118, 393, 412
MalI GATC 3 cut(s) 267, 601, 773
MboI GATC 3 cut(s) 265, 599, 771
MboII GAAGA 7 cut(s) 133, 337, 518, 583, 595, 640, 742
MhlI GDGCHC 1 cut(s) 376
MlsI TGGCCA 1 cut(s) 36
MluCI AATT 8 cut(s) 25, 105, 125, 179, 415, 607, 634, 652
MluNI TGGCCA 1 cut(s) 36
MlyI GAGTC 1 cut(s) 121
MnlI CCTC 4 cut(s) 78, 124, 287, 706
Mox20I TGGCCA 1 cut(s) 36
Mph1103I ATGCAT 1 cut(s) 322
MroXI GAANNNNTTC 1 cut(s) 425
MscI TGGCCA 1 cut(s) 36
MseI TTAA 4 cut(s) 29, 378, 405, 579
Msp20I TGGCCA 1 cut(s) 36
Mva1269I GAATGC 1 cut(s) 322
MwoI GCNNNNNNNGC 1 cut(s) 341
NcoI CCATGG 1 cut(s) 45
NdeII GATC 3 cut(s) 265, 599, 771
NheI GCTAGC 1 cut(s) 340
NlaIII CATG 6 cut(s) 49, 100, 272, 499, 689, 785
NmeAIII GCCGAG 1 cut(s) 736
NsiI ATGCAT 1 cut(s) 322
NspI RCATGY 1 cut(s) 785
NspV TTCGAA 1 cut(s) 419
PagI TCATGA 2 cut(s) 96, 268
PciSI GCTCTTC 1 cut(s) 146
PctI GAATGC 1 cut(s) 322
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 1 cut(s) 190
PkrI GCNGC 2 cut(s) 706, 709
PleI GAGTC 1 cut(s) 120
PpsI GAGTC 1 cut(s) 120
Ppu21I YACGTR 1 cut(s) 413
PsiI TTATAA 1 cut(s) 768
Psp124BI GAGCTC 1 cut(s) 376
PspPI GGNCC 1 cut(s) 682
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
SacI GAGCTC 1 cut(s) 376
SapI GCTCTTC 1 cut(s) 146
SaqAI TTAA 4 cut(s) 29, 378, 405, 579
SatI GCNGC 2 cut(s) 705, 708
Sau3AI GATC 3 cut(s) 265, 599, 771
Sau96I GGNCC 1 cut(s) 682
SchI GAGTC 1 cut(s) 121
SduI GDGCHC 1 cut(s) 376
SfaNI GCATC 1 cut(s) 30
SfcI CTRYAG 1 cut(s) 196
SfuI TTCGAA 1 cut(s) 419
SinI GGWCC 1 cut(s) 682
SmlI CTYRAG 2 cut(s) 56, 434
SmoI CTYRAG 2 cut(s) 56, 434
SnaBI TACGTA 1 cut(s) 413
Sse9I AATT 8 cut(s) 25, 105, 125, 179, 415, 607, 634, 652
SsiI CCGC 2 cut(s) 85, 205
SspMI CTAG 1 cut(s) 341
SstI GAGCTC 1 cut(s) 376
StyI CCWWGG 3 cut(s) 45, 345, 678
TaiI ACGT 3 cut(s) 121, 396, 415
TaqI TCGA 2 cut(s) 419, 441
TasI AATT 8 cut(s) 25, 105, 125, 179, 415, 607, 634, 652
TfiI GAWTC 1 cut(s) 190
Tru1I TTAA 4 cut(s) 29, 378, 405, 579
Tru9I TTAA 4 cut(s) 29, 378, 405, 579
TscAI CASTG 2 cut(s) 256, 342
TseI GCWGC 2 cut(s) 704, 707
TspDTI ATGAA 7 cut(s) 85, 138, 381, 519, 525, 702, 714
TspGWI ACGGA 1 cut(s) 548
TspRI CASTG 2 cut(s) 256, 342
VpaK11BI GGWCC 1 cut(s) 682
XapI RAATTY 1 cut(s) 607
XceI RCATGY 1 cut(s) 785
XmiI GTMKAC 1 cut(s) 797
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 1 cut(s) 341
ZraI GACGTC 1 cut(s) 394
Zsp2I ATGCAT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.