Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0000356

Physical Location & Seq

Genomic Coordinates Reverse (-)

22579326 .. 22587890

Gene Structure 8565 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1386 bp

ATGATGTACCCTGATGCACAACAGCAACACCACCAACAACAGCACCCACAACATCAGCAGGAACATTCTCAACATCAGCAGCAGCATTTGCAACAACATCAGCAGCAGCATCTGCAACAACACCAGCAACAGCATTTGCAACTACACCAGCAACATCAGCAGCAAGGTGGAGAATTTCATAGGGGCCCGCCGCCACCGGCCCTAATGATGCGTCAGCCGTCGGCTTCTTCAACTAATATGCCCCCAGAGTATCACAACCAACCTGGGCTCGCTCCTCCTTATGATGCTCATGCTGACAGTTTTCCTGGAAAAAGGATGAAAAAGCTTACACACAGGAGGGCAGTTGATTATACTAGCACTGTCGTGCGATATATGCAGATGCGAATGTGGCAGCGCGATGCAAGGGATAGAACGGTATTGCAACCCACACCTGCAGCAGCAATTGATATGTTGCCTTCAGTTGCCTACTCAGATAATCCATCCACTAGCTTTGCTGCAAAGTTTGTACACACTTCTTTAAACAAGAATCGTTGTCCGATTAATCGGGTTTTGTGGACTCCTACAGGAAGACGTCTTATTACTGGCTCTCAAAGTGGGGAATTCACTCTTTGGAATGGACAATCATTTAACTTTGAAATGATTCTTCAGGCTCATGATCAAGCAATCAGGTCTATGGTGTGGAGTCATAATGACAATTGGATGGTCTCTGGTGATGATGGCGGTGCAATAAAGTATTGGCAAAACAACATGAATAATGTAAAGGCAAATAAATCTGCTCATAAAGAATCTGTTCGCGACTTAAGCTTCTGCAAGACTGACTTGAAATTCTGTTCCTGCTCCGATGATAATACTGTTAAAGTTTGGGACTTTGCACGGTGCCAAGAAGAGCGCACACTGACTGGCCATGGTTGGGATGTGAAGAGTGTTGACTGGCACCCTACTAAATCTCTACTAGTTTCAGGTGGGAAAGACAGTCTTGTTAAACTTTGGGATGCTAAGTCAGGGCGAGAACTCTGTTCGTTTCATGGCCATAAAAATATGGTGCTCTCGGTTAAATGGAACCAAAATGGTAACTGGGTGCTAACTGCTTCGAAGGATCAAATCATCAAGGTTTATGACATAAGGGCCATGAAAGAACTTGCATCCTTCCGCGGGCATCGAAAGGATGTGACTGCTCTGGCTTGGCATCCGTTTCATGAAGAATATTTTGTTAGTGGGAGTTTTGATGGATCCATTTACCATTGGATTGTTGGGCATGAAACTCCCCAGGTTGAAATTCCCAATGCACATGATCACAGTGTGTGGGATCTTGCATGGCATCCTATCGGTTATCTTCTCTGCAGTGGTAGCAATCGATCACACTACGAAGTTTTGGTGCAGAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

WD40

Protein Analysis

462

Amino Acids

53.23

Weight (kDa)

8.7

Isoelectric Point (pI)

44.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_MABP1-WDR62_2nd	PF24782	127 - 415	8.8e-14	MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	167 - 335	8.1e-12	WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz	PF24807	171 - 340	1.8e-24	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	171 - 348	5.4e-26	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	172 - 331	1.4e-24	WDR3 first beta-propeller domain
Beta-prop_EIPR1	PF23609	172 - 330	1.3e-06	EIPR1 beta-propeller
Beta-prop_IFT140_1st	PF23383	174 - 261	2.5e-10	IFT140 first beta-propeller
Beta-prop_WDR5	PF25175	174 - 251	1.3e-07	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	178 - 290	5.7e-06	WDR36/Utp21 first beta-propeller
WD40_Gbeta	PF25391	180 - 383	3.3e-19	G protein beta WD-40 repeat protein
Beta-prop_EML	PF23409	186 - 258	6.2e-07	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	211 - 299	8.9e-06	CAF1B/HIR1 beta-propeller domain
EIF3I	PF24805	214 - 295	1.6e-08	EIF3I
WD40_Prp19	PF24814	229 - 382	1.5e-24	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	242 - 373	1.8e-29	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	243 - 332	1.2e-09	WDR3 second beta-propeller domain
WDR55	PF24796	251 - 418	1.1e-16	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	255 - 416	3.1e-17	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	258 - 289	2.7e-06	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	262 - 341	6.9e-10	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	271 - 418	1.8e-18	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	275 - 361	1.6e-06	WDR90/POC16, second beta-propeller
Beta-prop_SCAP	PF24017	278 - 374	8e-06	SCAP Beta-propeller
Beta-prop_WDR3_1st	PF25173	295 - 415	1.6e-25	WDR3 first beta-propeller domain
WD40	PF00400	295 - 331	2.8e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	297 - 378	3.4e-11	CAF1B/HIR1 beta-propeller domain
EIF3I	PF24805	298 - 447	5.8e-10	EIF3I
Beta-prop_WDR3_2nd	PF25172	298 - 418	7.3e-14	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	299 - 405	3.8e-09	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin	PF25460	305 - 414	9.4e-07	Aladin seven-bladed propeller
Beta-prop_TEP1_2nd	PF25047	306 - 391	1.1e-09	TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	321 - 449	3.9e-08	WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st	PF25173	321 - 455	4.4e-23	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	328 - 456	2.4e-20	CDC20/Fizzy WD40 domain
WD40	PF00400	336 - 373	1.6e-08	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	341 - 450	2.4e-17	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	344 - 453	3.8e-19	THOC3 beta-propeller domain
WD40_Prp19	PF24814	348 - 453	3.3e-15	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	348 - 442	1.1e-10	WDHD1 first WD40 domain
WD40	PF00400	378 - 415	1.1e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000356)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca	FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica	MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica	Prupe.2G034300_v2.0.a1
pyrus_communis	pycom02g24420 pycom07g02650
rosa_chinensis	RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata	RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora	Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii	Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa	Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha	Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana	Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	439
AatII	GACGTC	1 cut(s)	574
Acc36I	ACCTGC	1 cut(s)	439
AccB1I	GGYRCC	2 cut(s)	876, 933
AccII	CGCG	3 cut(s)	396, 795, 1152
AciI	CCGC	5 cut(s)	188, 191, 720, 1150, 1152
AclWI	GGATC	4 cut(s)	1104, 1224, 1237, 1314
AcoI	YGGCCR	2 cut(s)	901, 1027
AcsI	RAATTY	4 cut(s)	173, 599, 824, 1275
AcuI	CTGAAG	2 cut(s)	441, 629
AcyI	GRCGYC	1 cut(s)	571
AdeI	CACNNNGTG	1 cut(s)	1300
AfaI	GTAC	2 cut(s)	8, 507
AfiI	CCNNNNNNNGG	2 cut(s)	910, 1152
AflII	CTTAAG	1 cut(s)	799
AgsI	TTSAA	4 cut(s)	231, 635, 823, 1274
AhlI	ACTAGT	1 cut(s)	952
AjnI	CCWGG	3 cut(s)	262, 304, 1266
AleI	CACNNNNGTG	1 cut(s)	362
AluBI	AGCT	3 cut(s)	325, 489, 804
AluI	AGCT	3 cut(s)	325, 489, 804
Alw21I	GWGCWC	1 cut(s)	1047
Alw26I	GTCTC	1 cut(s)	709
AlwI	GGATC	4 cut(s)	1104, 1224, 1237, 1314
AlwNI	CAGNNNCTG	1 cut(s)	112
AoxI	GGCC	5 cut(s)	184, 198, 901, 1027, 1125
ApaI	GGGCCC	1 cut(s)	188
ApeKI	GCWGC	9 cut(s)	79, 82, 103, 106, 160, 391, 434, 437, 494
ApoI	RAATTY	4 cut(s)	173, 599, 824, 1275
AseI	ATTAAT	1 cut(s)	540
Asp700I	GAANNNNTTC	2 cut(s)	639, 789
AspLEI	GCGC	2 cut(s)	396, 891
AspS9I	GGNCC	4 cut(s)	184, 185, 199, 1125
AsuHPI	GGTGA	1 cut(s)	722
AsuII	TTCGAA	1 cut(s)	1091
BaeGI	GKGCMC	1 cut(s)	188
BalI	TGGCCA	2 cut(s)	903, 1029
BamHI	GGATCC	1 cut(s)	1229
BanI	GGYRCC	2 cut(s)	876, 933
BanII	GRGCYC	2 cut(s)	188, 270
BbsI	GAAGAC	1 cut(s)	574
Bbv12I	GWGCWC	1 cut(s)	1047
BbvI	GCAGC	9 cut(s)	91, 94, 115, 118, 172, 403, 446, 449, 481
BccI	CCATC	4 cut(s)	487, 694, 710, 1220
BceAI	ACGGC	1 cut(s)	202
BciT130I	CCWGG	3 cut(s)	264, 306, 1268
BclI	TGATCA	2 cut(s)	655, 1291
BcoDI	GTCTC	1 cut(s)	709
BcuI	ACTAGT	1 cut(s)	952
BfaI	CTAG	3 cut(s)	354, 486, 953
BfmI	CTRYAG	3 cut(s)	432, 561, 1339
BfrI	CTTAAG	1 cut(s)	799
BfuAI	ACCTGC	1 cut(s)	439
Bme1390I	CCNGG	3 cut(s)	264, 306, 1268
BmgT120I	GGNCC	4 cut(s)	184, 185, 199, 1125
BmiI	GGNNCC	6 cut(s)	185, 186, 878, 935, 1061, 1231
BmrFI	CCNGG	3 cut(s)	264, 306, 1268
BmrI	ACTGGG	1 cut(s)	1084
BmuI	ACTGGG	1 cut(s)	1084
BpiI	GAAGAC	1 cut(s)	574
Bpu14I	TTCGAA	1 cut(s)	1091
Bsa29I	ATCGAT	1 cut(s)	1354
BsaHI	GRCGYC	1 cut(s)	571
BsaI	GGTCTC	1 cut(s)	709
BsaJI	CCNNGG	4 cut(s)	263, 904, 1150, 1266
Bsc4I	CCNNNNNNNGG	2 cut(s)	910, 1152
Bse118I	RCCGGY	1 cut(s)	196
Bse1I	ACTGG	4 cut(s)	586, 904, 935, 1079
BseBI	CCWGG	3 cut(s)	264, 306, 1268
BseCI	ATCGAT	1 cut(s)	1354
BseDI	CCNNGG	4 cut(s)	263, 904, 1150, 1266
BseGI	GGATG	9 cut(s)	321, 479, 705, 919, 997, 1142, 1171, 1186, 1318
BseLI	CCNNNNNNNGG	2 cut(s)	910, 1152
BseMII	CTCAG	1 cut(s)	483
BseNI	ACTGG	4 cut(s)	586, 904, 935, 1079
BseRI	GAGGAG	1 cut(s)	264
BseSI	GKGCMC	1 cut(s)	188
BseXI	GCAGC	9 cut(s)	91, 94, 115, 118, 172, 403, 446, 449, 481
Bsh1236I	CGCG	3 cut(s)	396, 795, 1152
BshFI	GGCC	5 cut(s)	186, 200, 903, 1029, 1127
BshNI	GGYRCC	2 cut(s)	876, 933
BshVI	ATCGAT	1 cut(s)	1354
BsiHKAI	GWGCWC	1 cut(s)	1047
BsiSI	CCGG	1 cut(s)	197
BslFI	GGGAC	1 cut(s)	878
BslI	CCNNNNNNNGG	2 cut(s)	910, 1152
BsmAI	GTCTC	1 cut(s)	709
BsmFI	GGGAC	1 cut(s)	878
BsnI	GGCC	5 cut(s)	186, 200, 903, 1029, 1127
Bso31I	GGTCTC	1 cut(s)	709
Bsp119I	TTCGAA	1 cut(s)	1091
Bsp120I	GGGCCC	1 cut(s)	184
Bsp1286I	GDGCHC	3 cut(s)	188, 270, 1047
Bsp1407I	TGTACA	1 cut(s)	505
Bsp143I	GATC	6 cut(s)	655, 1096, 1229, 1291, 1306, 1355
Bsp19I	CCATGG	1 cut(s)	904
Bsp68I	TCGCGA	1 cut(s)	795
BspACI	CCGC	5 cut(s)	188, 191, 720, 1150, 1152
BspANI	GGCC	5 cut(s)	186, 200, 903, 1029, 1127
BspCNI	CTCAG	1 cut(s)	482
BspDI	ATCGAT	1 cut(s)	1354
BspFNI	CGCG	3 cut(s)	396, 795, 1152
BspHI	TCATGA	2 cut(s)	652, 1195
BspLI	GGNNCC	6 cut(s)	185, 186, 878, 935, 1061, 1231
BspMAI	CTGCAG	2 cut(s)	436, 1343
BspMI	ACCTGC	1 cut(s)	439
BspPI	GGATC	4 cut(s)	1104, 1224, 1237, 1314
BspQI	GCTCTTC	1 cut(s)	879
BspT104I	TTCGAA	1 cut(s)	1091
BspT107I	GGYRCC	2 cut(s)	876, 933
BspTI	CTTAAG	1 cut(s)	799
BspTNI	GGTCTC	1 cut(s)	709
BsrFI	RCCGGY	1 cut(s)	196
BsrGI	TGTACA	1 cut(s)	505
BsrI	ACTGG	4 cut(s)	586, 904, 935, 1079
BssAI	RCCGGY	1 cut(s)	196
BssECI	CCNNGG	4 cut(s)	263, 904, 1150, 1266
BssMI	GATC	6 cut(s)	655, 1096, 1229, 1291, 1306, 1355
BssNI	GRCGYC	1 cut(s)	571
BssT1I	CCWWGG	1 cut(s)	904
Bst2UI	CCWGG	3 cut(s)	264, 306, 1268
Bst4CI	ACNGT	7 cut(s)	299, 361, 415, 853, 876, 974, 1298
Bst6I	CTCTTC	2 cut(s)	879, 914
BstACI	GRCGYC	1 cut(s)	571
BstAFI	CTTAAG	1 cut(s)	799
BstAPI	GCANNNNNTGC	2 cut(s)	88, 112
BstAUI	TGTACA	1 cut(s)	505
BstBI	TTCGAA	1 cut(s)	1091
BstC8I	GCNNGC	3 cut(s)	188, 270, 1154
BstDEI	CTNAG	2 cut(s)	469, 996
BstDSI	CCRYGG	2 cut(s)	904, 1150
BstF5I	GGATG	9 cut(s)	321, 479, 705, 919, 997, 1142, 1171, 1186, 1318
BstFNI	CGCG	3 cut(s)	396, 795, 1152
BstHHI	GCGC	2 cut(s)	396, 891
BstKTI	GATC	6 cut(s)	658, 1099, 1232, 1294, 1309, 1358
BstMAI	GTCTC	1 cut(s)	709
BstMBI	GATC	6 cut(s)	655, 1096, 1229, 1291, 1306, 1355
BstMWI	GCNNNNNNNGC	7 cut(s)	88, 112, 157, 373, 388, 801, 1347
BstNI	CCWGG	3 cut(s)	264, 306, 1268
BstSCI	CCNGG	3 cut(s)	262, 304, 1266
BstSFI	CTRYAG	3 cut(s)	432, 561, 1339
BstSLI	GKGCMC	1 cut(s)	188
BstUI	CGCG	3 cut(s)	396, 795, 1152
BstV1I	GCAGC	9 cut(s)	91, 94, 115, 118, 172, 403, 446, 449, 481
BstV2I	GAAGAC	1 cut(s)	574
BstX2I	RGATCY	2 cut(s)	1229, 1306
BstYI	RGATCY	2 cut(s)	1229, 1306
Bsu15I	ATCGAT	1 cut(s)	1354
BsuRI	GGCC	5 cut(s)	186, 200, 903, 1029, 1127
BsuTUI	ATCGAT	1 cut(s)	1354
BtgI	CCRYGG	2 cut(s)	904, 1150
BtgZI	GCGATG	1 cut(s)	411
BtsCI	GGATG	9 cut(s)	321, 479, 705, 919, 997, 1142, 1171, 1186, 1318
BtsI	GCAGTG	1 cut(s)	1348
BtsIMutI	CAGTG	4 cut(s)	357, 893, 1303, 1348
BtuMI	TCGCGA	1 cut(s)	795
BveI	ACCTGC	1 cut(s)	439
Cac8I	GCNNGC	3 cut(s)	188, 270, 1154
CaiI	CAGNNNCTG	1 cut(s)	112
CciI	TCATGA	2 cut(s)	652, 1195
CfoI	GCGC	2 cut(s)	396, 891
Cfr10I	RCCGGY	1 cut(s)	196
Cfr13I	GGNCC	4 cut(s)	184, 185, 199, 1125
Cfr42I	CCGCGG	1 cut(s)	1153
ClaI	ATCGAT	1 cut(s)	1354
CseI	GACGC	1 cut(s)	200
Csp6I	GTAC	2 cut(s)	7, 506
CspCI	CAANNNNNGTGG	2 cut(s)	472, 507
CviQI	GTAC	2 cut(s)	7, 506
DdeI	CTNAG	2 cut(s)	469, 996
DpnI	GATC	6 cut(s)	657, 1098, 1231, 1293, 1308, 1357
DpnII	GATC	6 cut(s)	655, 1096, 1229, 1291, 1306, 1355
DraI	TTTAAA	1 cut(s)	519
DraIII	CACNNNGTG	1 cut(s)	1300
EaeI	YGGCCR	2 cut(s)	901, 1027
Eam1104I	CTCTTC	2 cut(s)	879, 914
EarI	CTCTTC	2 cut(s)	879, 914
Eco130I	CCWWGG	1 cut(s)	904
Eco24I	GRGCYC	2 cut(s)	188, 270
Eco31I	GGTCTC	1 cut(s)	709
Eco57I	CTGAAG	2 cut(s)	441, 629
EcoO109I	RGGNCCY	1 cut(s)	184
EcoRI	GAATTC	1 cut(s)	599
EcoRII	CCWGG	3 cut(s)	262, 304, 1266
EcoT14I	CCWWGG	1 cut(s)	904
EcoT38I	GRGCYC	2 cut(s)	188, 270
ErhI	CCWWGG	1 cut(s)	904
FalI	AAGNNNNNCTT	4 cut(s)	803, 835, 960, 992
FaqI	GGGAC	1 cut(s)	878
FauI	CCCGC	2 cut(s)	195, 1145
FbaI	TGATCA	2 cut(s)	655, 1291
FokI	GGATG	9 cut(s)	328, 466, 712, 926, 1004, 1129, 1173, 1178, 1305
FriOI	GRGCYC	2 cut(s)	188, 270
FspBI	CTAG	3 cut(s)	354, 486, 953
GlaI	GCGC	2 cut(s)	395, 890
HaeIII	GGCC	5 cut(s)	186, 200, 903, 1029, 1127
HapII	CCGG	1 cut(s)	197
HgaI	GACGC	1 cut(s)	200
HhaI	GCGC	2 cut(s)	396, 891
Hin1I	GRCGYC	1 cut(s)	571
Hin6I	GCGC	2 cut(s)	394, 889
HinP1I	GCGC	2 cut(s)	394, 889
HincII	GTYRAC	1 cut(s)	928
HindII	GTYRAC	1 cut(s)	928
HindIII	AAGCTT	2 cut(s)	323, 802
HinfI	GANTC	5 cut(s)	526, 556, 640, 682, 785
HpaII	CCGG	1 cut(s)	197
HphI	GGTGA	1 cut(s)	722
Hpy166II	GTNNAC	3 cut(s)	508, 555, 928
Hpy188I	TCNGA	3 cut(s)	472, 537, 841
Hpy188III	TCNNGA	3 cut(s)	653, 794, 1196
Hpy8I	GTNNAC	3 cut(s)	508, 555, 928
Hpy99I	CGWCG	1 cut(s)	223
HpyAV	CCTTC	3 cut(s)	465, 1087, 1156
HpyCH4III	ACNGT	7 cut(s)	299, 361, 415, 853, 876, 974, 1298
HpyCH4IV	ACGT	1 cut(s)	571
HpyF10VI	GCNNNNNNNGC	7 cut(s)	88, 112, 157, 373, 388, 801, 1347
HpyF3I	CTNAG	2 cut(s)	469, 996
HpySE526I	ACGT	1 cut(s)	571
Hsp92I	GRCGYC	1 cut(s)	571
HspAI	GCGC	2 cut(s)	394, 889
Ksp22I	TGATCA	2 cut(s)	655, 1291
KspI	CCGCGG	1 cut(s)	1153
Kzo9I	GATC	6 cut(s)	655, 1096, 1229, 1291, 1306, 1355
LguI	GCTCTTC	1 cut(s)	879
LmnI	GCTCC	2 cut(s)	277, 842
Lsp1109I	GCAGC	9 cut(s)	91, 94, 115, 118, 172, 403, 446, 449, 481
MaeI	CTAG	3 cut(s)	354, 486, 953
MaeII	ACGT	1 cut(s)	571
MaeIII	GTNAC	2 cut(s)	1070, 1168
MalI	GATC	6 cut(s)	657, 1098, 1231, 1293, 1308, 1357
MboI	GATC	6 cut(s)	655, 1096, 1229, 1291, 1306, 1355
MboII	GAAGA	7 cut(s)	219, 579, 635, 896, 931, 1211, 1325
MfeI	CAATTG	2 cut(s)	441, 694
MflI	RGATCY	2 cut(s)	1229, 1306
MhlI	GDGCHC	3 cut(s)	188, 270, 1047
MlsI	TGGCCA	2 cut(s)	903, 1029
MluCI	AATT	6 cut(s)	173, 441, 599, 694, 824, 1275
MluNI	TGGCCA	2 cut(s)	903, 1029
MlyI	GAGTC	2 cut(s)	550, 691
MnlI	CCTC	2 cut(s)	285, 330
Mox20I	TGGCCA	2 cut(s)	903, 1029
MroXI	GAANNNNTTC	2 cut(s)	639, 789
MscI	TGGCCA	2 cut(s)	903, 1029
MseI	TTAA	7 cut(s)	518, 540, 627, 800, 855, 981, 1053
MslI	CAYNNNNRTG	1 cut(s)	362
Msp20I	TGGCCA	2 cut(s)	903, 1029
MspA1I	CMGCKG	1 cut(s)	1152
MspCI	CTTAAG	1 cut(s)	799
MspI	CCGG	1 cut(s)	197
MspR9I	CCNGG	3 cut(s)	264, 306, 1268
MunI	CAATTG	2 cut(s)	441, 694
MvaI	CCWGG	3 cut(s)	264, 306, 1268
MvnI	CGCG	3 cut(s)	396, 795, 1152
MwoI	GCNNNNNNNGC	7 cut(s)	88, 112, 157, 373, 388, 801, 1347
NcoI	CCATGG	1 cut(s)	904
NdeII	GATC	6 cut(s)	655, 1096, 1229, 1291, 1306, 1355
NlaIV	GGNNCC	6 cut(s)	185, 186, 878, 935, 1061, 1231
NmuCI	GTSAC	1 cut(s)	1168
NruI	TCGCGA	1 cut(s)	795
NspV	TTCGAA	1 cut(s)	1091
OliI	CACNNNNGTG	1 cut(s)	362
PagI	TCATGA	2 cut(s)	652, 1195
PaqCI	CACCTGC	1 cut(s)	439
PciSI	GCTCTTC	1 cut(s)	879
PdmI	GAANNNNTTC	2 cut(s)	639, 789
PfeI	GAWTC	3 cut(s)	526, 640, 785
PfoI	TCCNGGA	1 cut(s)	304
PleI	GAGTC	2 cut(s)	550, 690
PpsI	GAGTC	2 cut(s)	550, 690
PshBI	ATTAAT	1 cut(s)	540
Psp6I	CCWGG	3 cut(s)	262, 304, 1266
PspGI	CCWGG	3 cut(s)	262, 304, 1266
PspN4I	GGNNCC	6 cut(s)	185, 186, 878, 935, 1061, 1231
PspOMI	GGGCCC	1 cut(s)	184
PspPI	GGNCC	4 cut(s)	184, 185, 199, 1125
PstI	CTGCAG	2 cut(s)	436, 1343
PstNI	CAGNNNCTG	1 cut(s)	112
PsuI	RGATCY	2 cut(s)	1229, 1306
RruI	TCGCGA	1 cut(s)	795
RsaI	GTAC	2 cut(s)	8, 507
RsaNI	GTAC	2 cut(s)	7, 506
RseI	CAYNNNNRTG	1 cut(s)	362
SacII	CCGCGG	1 cut(s)	1153
SapI	GCTCTTC	1 cut(s)	879
SaqAI	TTAA	7 cut(s)	518, 540, 627, 800, 855, 981, 1053
Sau3AI	GATC	6 cut(s)	655, 1096, 1229, 1291, 1306, 1355
Sau96I	GGNCC	4 cut(s)	184, 185, 199, 1125
SchI	GAGTC	2 cut(s)	550, 691
ScrFI	CCNGG	3 cut(s)	264, 306, 1268
SduI	GDGCHC	3 cut(s)	188, 270, 1047
SfcI	CTRYAG	3 cut(s)	432, 561, 1339
Sfr303I	CCGCGG	1 cut(s)	1153
SfuI	TTCGAA	1 cut(s)	1091
SgrBI	CCGCGG	1 cut(s)	1153
SmiMI	CAYNNNNRTG	1 cut(s)	362
SmlI	CTYRAG	1 cut(s)	799
SmoI	CTYRAG	1 cut(s)	799
SpeI	ACTAGT	1 cut(s)	952
Sse9I	AATT	6 cut(s)	173, 441, 599, 694, 824, 1275
SsiI	CCGC	5 cut(s)	188, 191, 720, 1150, 1152
SspI	AATATT	1 cut(s)	1205
SspMI	CTAG	3 cut(s)	354, 486, 953
StyD4I	CCNGG	3 cut(s)	262, 304, 1266
StyI	CCWWGG	1 cut(s)	904
TaaI	ACNGT	7 cut(s)	299, 361, 415, 853, 876, 974, 1298
TaiI	ACGT	1 cut(s)	574
TaqI	TCGA	3 cut(s)	1091, 1159, 1354
TasI	AATT	6 cut(s)	173, 441, 599, 694, 824, 1275
TatI	WGTACW	1 cut(s)	505
TauI	GCSGC	1 cut(s)	193
TfiI	GAWTC	3 cut(s)	526, 640, 785
Tru1I	TTAA	7 cut(s)	518, 540, 627, 800, 855, 981, 1053
Tru9I	TTAA	7 cut(s)	518, 540, 627, 800, 855, 981, 1053
TscAI	CASTG	4 cut(s)	364, 900, 1303, 1348
TseFI	GTSAC	1 cut(s)	1168
TseI	GCWGC	9 cut(s)	79, 82, 103, 106, 160, 391, 434, 437, 494
Tsp45I	GTSAC	1 cut(s)	1168
TspDTI	ATGAA	8 cut(s)	167, 332, 764, 1013, 1145, 1184, 1212, 1272
TspGWI	ACGGA	1 cut(s)	1179
TspRI	CASTG	4 cut(s)	364, 900, 1303, 1348
Vha464I	CTTAAG	1 cut(s)	799
VspI	ATTAAT	1 cut(s)	540
XapI	RAATTY	4 cut(s)	173, 599, 824, 1275
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1247
XmnI	GAANNNNTTC	2 cut(s)	639, 789
XspI	CTAG	3 cut(s)	354, 486, 953
ZraI	GACGTC	1 cut(s)	572

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.