Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0000356

Physical Location & Seq

Genomic Coordinates Forward (+)

2177150 .. 2186706

Gene Structure 9557 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2223 bp

ATGATGTACCCTGATCCACAACAGCAACACCACCAACAGCAGCTTCAGCAGCAGCACCCACAACATCAGCAGCAGCAGCATCCCCAACAACACCAGCAATATCAGCAGCAACATCCGCAGCAAGGTGGAGAATTTCATAGGGGCCCGCCGCCGCCGGCCCCAATGATGCGTCAGCCGTCGGCCTCTTCAACTAATATGCCTCCAGAGTATCACAACCAACCTGGGCTGGCTCCTCCTCTTCCTCCTTATGATGCTCATGGTGACAGTTTTCCTGGAAAAAGGATGAGAAAGCTTACACACAGGAGGGCAGTTGATTATACTAGCACTGTCGTGCGATATATGCAGATTCGAATGTGGCAACGTGATGCAAGGGATAGAACGGTATTGCAACCCACACCTGCAGCAGCAATTGATATGTTGCCACCAGTTGCCTACACAGATAATCCATCCACTAGCTTTGCTGCAAAGTTTGTGCACACATCTTTAAACAAGAATCGTTGTTCTATTAATCGTGTTTTGTGGACTCCTACAGGAAGGCGTCTTATTACTGGCTCTCAAAGTGGGGAATTCACTCTTTGGAATGGACAGTCATTTAATTTTGAAATGATTCTTCAGGATGATGATCAAGCAATCAGGTCTATGGTATGGAGTCATAATGACAATTGGATGGTATCTGGTGATGATGGCGGTGCAATAAAGTACTGGCAGAACAACATGAATAATGTAAAGGCCAATAAATCTGCTCATAAAGAATCTGTTCGTGACTTAAGCTTCTGCAAGACTGACTTGAAGTTCTGTTCCTGCTCTGATGATAATACTGTTAAAGTTTGGGACTTTGCACGGTGCCAAGAAGAGCGCACATTGACTGGCCATGGTTGGGATGTGAAGAGTGTTGACTGGCACCCTACTAAATCTCTGCTAGTTTCAGGTGGGAAAGACAATCTTGTTAAACTTTGGGATGCTAAGTCAGGACGAGAACTCTGTTCATTTCATGGCCATAAAAATATGGTGCTCTCCGTTAAATGGAACCAAAATGGTAACTGGGTTCTAACTGCTTCGAAGGATCAAATCATCAAGGTTTATGACATAAGGGCCATGAAGGAACTTGCATCCTTCCGCGGGCATCGAAAGGACGTGACTGCTCTGGCTTGGCATCCGTTTCATGAAGAATATTTTGTCAGTGGGAGTTCTGATGGATCCATTTACCATTGGCTTGTTGGGCATGAAACTCCCCAGGTTGAAATTCTTAATGCACATGATCATAGTGTGTGGGATCTCGCTTGGCATCCTATTGGTTATCTTCTCTGCAGTGGTAGCAATGATCACACTACGAAGTTTTGGTGCAGAAATAGGCCAGGAGACACTGGTCGGGATAAATTTAACCCCGGCCAAAATCAAGGTTATGGTGAGCAAAACCCTGCTTTTGGTGGTCGCATGACTGGTAATTTTCCAGTACCCGAAGGACCACCAACTCCTGGACCACTTGTTCCTGGGTTGACTCGAAATGAAGGAACTATTCCTGGTGTTGGAGTTGCTATGCCATTCTCTCTTGATGCATCAAATCAGGGGGAGCAGCAACAACCTCATGCACTTTCGATGCCTTTAGGTGCTCCTCCTCTCCCACCTGGTCCCCACCCCTCTCTTCTTGCTGTGAATCAGCAGCAACAATATCAGCAAAATCCTCAACAGATGCCACAACAGCAACACCAACACCAAGGTCACCCGCAACAAATGCCCCCTATGCCTATGGCACCTCCAAATATGCCACAGCTGCAGCCTCCATCCCATTTACCCTTGCTGCCACATCCTCATTTACATCGACCGCCACCCCAAATGCCCCCACTTGGTATGCCATCTTCAGGGCCGGGATCTTTGTCTATGCCTTCATCGGTTCCTACCTCACATTCAATGCCAATGGGAATTCAAGGTTCAATGAATCAGATGGGTCACCCATTGCCACAAGGTCATTTTATGGGCATGAACCCAATGCACTCGGGATCTCTACCTACAAGCAGTGGAGGTTTTCCAAATGGTATGCCAAACATGCAGGGTCCAACAAATGCCAGTGGACCTCAAATGTATCCTCAGGGTGGCACCTTCAATCGGGGTCAACCTGGGCAAATTCCCATGCATCCGGGGTATAATCCTTACCAGGCTAGGGGTCAATCTGGTATGCCCCAACCTCCACCAGGGCCACCTCCACACGGGCAAACACCTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

WD40

Protein Analysis

741

Amino Acids

82.42

Weight (kDa)

8.63

Isoelectric Point (pI)

53.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_MABP1-WDR62_2nd	PF24782	116 - 404	2.6e-12	MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	156 - 320	7.7e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	157 - 279	2.8e-06	WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz	PF24807	160 - 329	3.7e-23	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	160 - 337	3e-25	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	161 - 316	3.2e-20	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	162 - 240	5e-06	WDR5 beta-propeller domain
Beta-prop_IFT140_1st	PF23383	162 - 251	6.5e-09	IFT140 first beta-propeller
WD40_Gbeta	PF25391	167 - 372	3.9e-18	G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1	PF24105	204 - 302	7.1e-06	CAF1B/HIR1 beta-propeller domain
EIF3I	PF24805	204 - 283	4.9e-08	EIF3I
WD40_Prp19	PF24814	219 - 382	9.1e-27	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	225 - 330	1.1e-10	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	231 - 364	8.3e-30	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	232 - 321	9.6e-10	WDR3 second beta-propeller domain
WDR55	PF24796	240 - 423	2.6e-17	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	244 - 405	6e-17	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	247 - 278	4.8e-06	WD domain, G-beta repeat
Beta-prop_EML_2	PF23414	260 - 385	1.3e-17	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	264 - 350	3.9e-06	WDR90/POC16, second beta-propeller
Beta-prop_WDR3_1st	PF25173	271 - 404	1.6e-29	WDR3 first beta-propeller domain
WD40	PF00400	284 - 320	2.3e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	286 - 367	6e-12	CAF1B/HIR1 beta-propeller domain
EIF3I	PF24805	287 - 446	5.4e-12	EIF3I
Beta-prop_WDR3_2nd	PF25172	287 - 407	4.5e-14	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	288 - 394	1.2e-08	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin	PF25460	294 - 404	4.9e-07	Aladin seven-bladed propeller
Beta-prop_TEP1_2nd	PF25047	295 - 380	2e-09	TEP-1 second beta-propeller
Beta-prop_WDR19_1st	PF23389	298 - 444	1.3e-06	WDR19 first beta-propeller
Beta-prop_EML_2	PF23414	301 - 448	1.5e-15	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	312 - 447	1.5e-25	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	315 - 448	1.3e-09	WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz	PF24807	317 - 449	4.4e-23	CDC20/Fizzy WD40 domain
WD40	PF00400	325 - 362	2.8e-08	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	331 - 448	2.1e-21	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	333 - 448	6e-22	THOC3 beta-propeller domain
Beta-prop_EIPR1	PF23609	333 - 402	9.9e-06	EIPR1 beta-propeller
WD40_Prp19	PF24814	336 - 447	3.7e-18	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	337 - 431	7.8e-11	WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd	PF23393	349 - 440	5.3e-06	WDR90/POC16, second beta-propeller
WD40	PF00400	367 - 404	1.8e-06	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	371 - 446	6.7e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	379 - 450	9.1e-06	TEP-1 second beta-propeller
WD40	PF00400	413 - 447	2.6e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000356)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13480 AT5G13480 AT5G13480 AT5G13480
fragaria_vesca	FvH4_4g16141 FvH4_5g25490 FvH4_5g25550 FvH4_6g22190 FvH4_6g23330 FvH4_7g02880
malus_domestica	MD02G1288700.v1.1 MD07G1038200.v1.1
prunus_persica	Prupe.2G034300_v2.0.a1
pyrus_communis	pycom02g24420 pycom07g02650
rosa_chinensis	RchiOBHm_Chr1g0323831 RchiOBHm_Chr3g0482821 RchiOBHm_Chr3g0482981 RchiOBHm_Chr3g0486211 RchiOBHm_Chr4g0404661 RchiOBHm_Chr5g0010661
rosa_laevigata	RLG00000012304 RLG00000017204 RLG00000017697 RLG00000020158 RLG00000030265 RLG00000031770 RLG00000035414
rosa_multiflora	Rmu_co8066382.1_g000001 Rmu_co8299121.1_g000001 Rmu_co8338957.1_g000001 Rmu_sc0001371.1_g000014 Rmu_sc0002634.1_g000006 Rmu_sc0004124.1_g000001 Rmu_sc0004540.1_g000008 Rmu_sc0006103.1_g000005 Rmu_sc0006571.1_g000003 Rmu_sc0006601.1_g000005 Rmu_sc0006601.1_g000008 Rmu_sc0006601.1_g000010 Rmu_sc0009883.1_g000005 Rmu_sc0011602.1_g000010 Rmu_sc0011602.1_g000011 Rmu_sc0012513.1_g000008 Rmu_sc0025336.1_g000001 Rmu_sc0026709.1_g000001 Rmu_ssc0000175.1_g000002 Rmu_ssc0000175.1_g000031
rosa_roxburghii	Rroxscaffold_1G00027770 Rroxscaffold_1G00065710 Rroxscaffold_3G00243370 Rroxscaffold_3G00243410 Rroxscaffold_3G00243440 Rroxscaffold_3G00243490 Rroxscaffold_4G00326390 Rroxscaffold_6G00398770 Rroxscaffold_6G00398910 Rroxscaffold_6G00398940
rosa_rugosa	Rorug01G0039800 Rorug01G0039900 Rorug03G0198000 Rorug03G0198100 Rorug03G0199200 Rorug04G0451600
rosa_samantha	Rh1AG057200 Rh1BG048200 Rh1CG058500 Rh1DG062300 Rh1DG062400 Rh2AG304100 Rh2CG002400 Rh2CG642500 Rh2DG328100 Rh3AG250000 Rh3BG285200 Rh3BG286000 Rh3CG284000 Rh3CG285400 Rh3CG307500 Rh3DG278700 Rh3DG279600 Rh3DG281000 Rh3DG303900 Rh4AG124300 Rh4BG118400 Rh4CG132400 Rh4CG132500 Rh4DG015900 Rh5AG083200 Rh5AG417500 Rh5AG538800 Rh5BG078500 Rh5BG447700 Rh5BG541600 Rh5BG541700 Rh5CG091200 Rh5CG564200 Rh5CG564300 Rh5DG078600 Rh5DG545800
rosa_wichuraiana	Rw0G000660 Rw0G022360 Rw1G004890 Rw3G022520 Rw3G022590 Rw3G022710 Rw3G024280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	406
Acc36I	ACCTGC	1 cut(s)	406
AccB1I	GGYRCC	4 cut(s)	843, 900, 1750, 2093
AccB7I	CCANNNNNTGG	1 cut(s)	1475
AccII	CGCG	1 cut(s)	1119
AciI	CCGC	9 cut(s)	116, 146, 149, 152, 687, 1117, 1119, 1724, 1823
AclWI	GGATC	7 cut(s)	8, 1071, 1191, 1204, 1281, 1876, 2005
AcoI	YGGCCR	3 cut(s)	868, 994, 1387
AcsI	RAATTY	6 cut(s)	131, 566, 1242, 1376, 1920, 2121
AcuI	CTGAAG	3 cut(s)	29, 596, 1842
AcyI	GRCGYC	1 cut(s)	538
AfaI	GTAC	3 cut(s)	8, 701, 1455
AflII	CTTAAG	1 cut(s)	766
AgsI	TTSAA	8 cut(s)	189, 602, 790, 1241, 1908, 1925, 1932, 2101
AjiI	CACGTC	1 cut(s)	1135
AjuI	GAANNNNNNNTTGG	2 cut(s)	27, 59
AleI	CACNNNNGTG	1 cut(s)	329
AloI	GAACNNNNNNTCC	2 cut(s)	1470, 1502
AluBI	AGCT	5 cut(s)	43, 292, 456, 771, 1771
AluI	AGCT	5 cut(s)	43, 292, 456, 771, 1771
Alw21I	GWGCWC	3 cut(s)	477, 1014, 1612
Alw26I	GTCTC	1 cut(s)	1353
Alw44I	GTGCAC	1 cut(s)	473
AlwI	GGATC	7 cut(s)	8, 1071, 1191, 1204, 1281, 1876, 2005
Ama87I	CYCGRG	1 cut(s)	1993
ApaI	GGGCCC	1 cut(s)	146
ApaLI	GTGCAC	1 cut(s)	473
ApoI	RAATTY	6 cut(s)	131, 566, 1242, 1376, 1920, 2121
AseI	ATTAAT	1 cut(s)	507
Asp700I	GAANNNNTTC	2 cut(s)	606, 756
AspLEI	GCGC	1 cut(s)	858
AsuC2I	CCSGG	3 cut(s)	1386, 1866, 2136
AsuHPI	GGTGA	5 cut(s)	272, 689, 1418, 1712, 1940
AsuII	TTCGAA	2 cut(s)	349, 1058
AvaI	CYCGRG	1 cut(s)	1993
AvaII	GGWCC	5 cut(s)	1463, 1478, 1628, 2051, 2069
AxyI	CCTNAGG	1 cut(s)	2085
BaeGI	GKGCMC	2 cut(s)	146, 477
BalI	TGGCCA	2 cut(s)	870, 996
BamHI	GGATCC	1 cut(s)	1196
BanI	GGYRCC	4 cut(s)	843, 900, 1750, 2093
BanII	GRGCYC	1 cut(s)	146
BarI	GAAGNNNNNNTAC	2 cut(s)	1840, 1872
Bbv12I	GWGCWC	3 cut(s)	477, 1014, 1612
BccI	CCATC	7 cut(s)	454, 661, 677, 1187, 1789, 1861, 1936
BceAI	ACGGC	1 cut(s)	160
BciVI	GTATCC	1 cut(s)	2091
BclI	TGATCA	3 cut(s)	622, 1258, 1321
BcnI	CCSGG	3 cut(s)	1386, 1866, 2136
BcoDI	GTCTC	1 cut(s)	1353
BfaI	CTAG	4 cut(s)	321, 453, 920, 2157
BfmI	CTRYAG	4 cut(s)	399, 528, 1306, 1772
BfrI	CTTAAG	1 cut(s)	766
BfuAI	ACCTGC	1 cut(s)	406
BfuI	GTATCC	1 cut(s)	2091
BmcAI	AGTACT	1 cut(s)	701
Bme18I	GGWCC	5 cut(s)	1463, 1478, 1628, 2051, 2069
BmeT110I	CYCGRG	1 cut(s)	1993
BmgBI	CACGTC	1 cut(s)	1135
BmrI	ACTGGG	1 cut(s)	1051
BmuI	ACTGGG	1 cut(s)	1051
BoxI	GACNNNNGTC	1 cut(s)	1365
BpmI	CTGGAG	1 cut(s)	186
Bpu14I	TTCGAA	2 cut(s)	349, 1058
BpuMI	CCSGG	3 cut(s)	1386, 1866, 2136
BsaBI	GATNNNNATC	1 cut(s)	621
BsaHI	GRCGYC	1 cut(s)	538
Bse118I	RCCGGY	1 cut(s)	154
Bse21I	CCTNAGG	1 cut(s)	2085
Bse3DI	GCAATG	2 cut(s)	1324, 1952
Bse8I	GATNNNNATC	1 cut(s)	621
BseJI	GATNNNNATC	1 cut(s)	621
BseMI	GCAATG	2 cut(s)	1324, 1952
BseMII	CTCAG	1 cut(s)	2099
BseRI	GAGGAG	4 cut(s)	222, 225, 1602, 1605
BseSI	GKGCMC	2 cut(s)	146, 477
BsgI	GTGCAG	1 cut(s)	1363
Bsh1236I	CGCG	1 cut(s)	1119
Bsh1285I	CGRYCG	1 cut(s)	1823
BshNI	GGYRCC	4 cut(s)	843, 900, 1750, 2093
BsiEI	CGRYCG	1 cut(s)	1823
BsiHKAI	GWGCWC	3 cut(s)	477, 1014, 1612
BsiHKCI	CYCGRG	1 cut(s)	1993
BsiSI	CCGG	4 cut(s)	155, 1386, 1865, 2135
BslFI	GGGAC	2 cut(s)	845, 1614
BsmAI	GTCTC	1 cut(s)	1353
BsmFI	GGGAC	2 cut(s)	845, 1614
BsoBI	CYCGRG	1 cut(s)	1993
Bsp119I	TTCGAA	2 cut(s)	349, 1058
Bsp120I	GGGCCC	1 cut(s)	142
Bsp1286I	GDGCHC	4 cut(s)	146, 477, 1014, 1612
Bsp143I	GATC	9 cut(s)	13, 622, 1063, 1196, 1258, 1273, 1321, 1868, 1997
Bsp19I	CCATGG	1 cut(s)	871
BspACI	CCGC	9 cut(s)	116, 146, 149, 152, 687, 1117, 1119, 1724, 1823
BspCNI	CTCAG	1 cut(s)	2098
BspFNI	CGCG	1 cut(s)	1119
BspHI	TCATGA	1 cut(s)	1162
BspMAI	CTGCAG	3 cut(s)	403, 1310, 1776
BspMI	ACCTGC	1 cut(s)	406
BspPI	GGATC	7 cut(s)	8, 1071, 1191, 1204, 1281, 1876, 2005
BspQI	GCTCTTC	1 cut(s)	846
BspT104I	TTCGAA	2 cut(s)	349, 1058
BspT107I	GGYRCC	4 cut(s)	843, 900, 1750, 2093
BspTI	CTTAAG	1 cut(s)	766
BsrDI	GCAATG	2 cut(s)	1324, 1952
BsrFI	RCCGGY	1 cut(s)	154
BssAI	RCCGGY	1 cut(s)	154
BssMI	GATC	9 cut(s)	13, 622, 1063, 1196, 1258, 1273, 1321, 1868, 1997
BssNI	GRCGYC	1 cut(s)	538
BssT1I	CCWWGG	2 cut(s)	871, 1714
Bst4CI	ACNGT	6 cut(s)	266, 328, 382, 588, 820, 843
Bst6I	CTCTTC	5 cut(s)	190, 243, 846, 881, 1647
BstACI	GRCGYC	1 cut(s)	538
BstAFI	CTTAAG	1 cut(s)	766
BstAPI	GCANNNNNTGC	1 cut(s)	1732
BstBI	TTCGAA	2 cut(s)	349, 1058
BstC8I	GCNNGC	4 cut(s)	146, 156, 228, 1121
BstDEI	CTNAG	3 cut(s)	963, 2085, 2217
BstDSI	CCRYGG	2 cut(s)	871, 1117
BstEII	GGTNACC	2 cut(s)	1718, 1946
BstENI	CCTNNNNNAGG	1 cut(s)	2187
BstFNI	CGCG	1 cut(s)	1119
BstHHI	GCGC	1 cut(s)	858
BstKTI	GATC	9 cut(s)	16, 625, 1066, 1199, 1261, 1276, 1324, 1871, 2000
BstMAI	GTCTC	1 cut(s)	1353
BstMBI	GATC	9 cut(s)	13, 622, 1063, 1196, 1258, 1273, 1321, 1868, 1997
BstMCI	CGRYCG	1 cut(s)	1823
BstNSI	RCATGY	1 cut(s)	2047
BstPAI	GACNNNNGTC	1 cut(s)	1365
BstPI	GGTNACC	2 cut(s)	1718, 1946
BstSFI	CTRYAG	4 cut(s)	399, 528, 1306, 1772
BstSLI	GKGCMC	2 cut(s)	146, 477
BstUI	CGCG	1 cut(s)	1119
BstX2I	RGATCY	4 cut(s)	1196, 1273, 1868, 1997
BstYI	RGATCY	4 cut(s)	1196, 1273, 1868, 1997
Bsu36I	CCTNAGG	1 cut(s)	2085
BsuI	GTATCC	1 cut(s)	2091
BtgI	CCRYGG	2 cut(s)	871, 1117
BtrI	CACGTC	1 cut(s)	1135
BtsI	GCAGTG	2 cut(s)	1315, 2020
BtsIMutI	CAGTG	6 cut(s)	324, 1186, 1315, 1362, 2020, 2071
BveI	ACCTGC	1 cut(s)	406
Cac8I	GCNNGC	4 cut(s)	146, 156, 228, 1121
CciI	TCATGA	1 cut(s)	1162
CfoI	GCGC	1 cut(s)	858
Cfr10I	RCCGGY	1 cut(s)	154
Cfr42I	CCGCGG	1 cut(s)	1120
CseI	GACGC	2 cut(s)	158, 527
CsiI	ACCWGGT	1 cut(s)	1624
Csp6I	GTAC	3 cut(s)	7, 700, 1454
CspCI	CAANNNNNGTGG	2 cut(s)	439, 474
CviQI	GTAC	3 cut(s)	7, 700, 1454
DdeI	CTNAG	3 cut(s)	963, 2085, 2217
DpnI	GATC	9 cut(s)	15, 624, 1065, 1198, 1260, 1275, 1323, 1870, 1999
DpnII	GATC	9 cut(s)	13, 622, 1063, 1196, 1258, 1273, 1321, 1868, 1997
DraI	TTTAAA	1 cut(s)	486
EaeI	YGGCCR	3 cut(s)	868, 994, 1387
Eam1104I	CTCTTC	5 cut(s)	190, 243, 846, 881, 1647
EarI	CTCTTC	5 cut(s)	190, 243, 846, 881, 1647
Eco130I	CCWWGG	2 cut(s)	871, 1714
Eco24I	GRGCYC	1 cut(s)	146
Eco47I	GGWCC	5 cut(s)	1463, 1478, 1628, 2051, 2069
Eco57I	CTGAAG	3 cut(s)	29, 596, 1842
Eco81I	CCTNAGG	1 cut(s)	2085
Eco88I	CYCGRG	1 cut(s)	1993
Eco91I	GGTNACC	2 cut(s)	1718, 1946
EcoNI	CCTNNNNNAGG	1 cut(s)	2187
EcoO109I	RGGNCCY	1 cut(s)	142
EcoO65I	GGTNACC	2 cut(s)	1718, 1946
EcoRI	GAATTC	2 cut(s)	566, 1920
EcoT14I	CCWWGG	2 cut(s)	871, 1714
EcoT22I	ATGCAT	2 cut(s)	1558, 2133
EcoT38I	GRGCYC	1 cut(s)	146
ErhI	CCWWGG	2 cut(s)	871, 1714
FalI	AAGNNNNNCTT	4 cut(s)	770, 802, 927, 959
FaqI	GGGAC	2 cut(s)	845, 1614
FauI	CCCGC	3 cut(s)	153, 1112, 1731
FbaI	TGATCA	3 cut(s)	622, 1258, 1321
FriOI	GRGCYC	1 cut(s)	146
FspBI	CTAG	4 cut(s)	321, 453, 920, 2157
GlaI	GCGC	1 cut(s)	857
GsuI	CTGGAG	1 cut(s)	186
HapII	CCGG	4 cut(s)	155, 1386, 1865, 2135
HgaI	GACGC	2 cut(s)	158, 527
HhaI	GCGC	1 cut(s)	858
Hin1I	GRCGYC	1 cut(s)	538
Hin6I	GCGC	1 cut(s)	856
HinP1I	GCGC	1 cut(s)	856
HincII	GTYRAC	3 cut(s)	895, 1497, 2111
HindII	GTYRAC	3 cut(s)	895, 1497, 2111
HindIII	AAGCTT	2 cut(s)	290, 769
HinfI	GANTC	9 cut(s)	346, 493, 523, 607, 649, 752, 1498, 1654, 1936
HpaII	CCGG	4 cut(s)	155, 1386, 1865, 2135
HphI	GGTGA	5 cut(s)	272, 689, 1418, 1712, 1940
Hpy166II	GTNNAC	6 cut(s)	475, 522, 895, 1497, 2069, 2111
Hpy188I	TCNGA	3 cut(s)	808, 1192, 1941
Hpy188III	TCNNGA	8 cut(s)	203, 614, 761, 969, 1163, 1370, 1550, 1995
Hpy8I	GTNNAC	6 cut(s)	475, 522, 895, 1497, 2069, 2111
Hpy99I	CGWCG	1 cut(s)	181
HpyAV	CCTTC	8 cut(s)	528, 1054, 1093, 1123, 1454, 1502, 1893, 2107
HpyCH4III	ACNGT	6 cut(s)	266, 328, 382, 588, 820, 843
HpyCH4IV	ACGT	2 cut(s)	361, 1134
HpyF3I	CTNAG	3 cut(s)	963, 2085, 2217
HpySE526I	ACGT	2 cut(s)	361, 1134
Hsp92I	GRCGYC	1 cut(s)	538
HspAI	GCGC	1 cut(s)	856
KroI	GCCGGC	1 cut(s)	154
KroNI	GCCGGC	1 cut(s)	156
Ksp22I	TGATCA	3 cut(s)	622, 1258, 1321
KspI	CCGCGG	1 cut(s)	1120
Kzo9I	GATC	9 cut(s)	13, 622, 1063, 1196, 1258, 1273, 1321, 1868, 1997
LguI	GCTCTTC	1 cut(s)	846
LmnI	GCTCC	3 cut(s)	235, 1570, 1615
MabI	ACCWGGT	1 cut(s)	1624
MaeI	CTAG	4 cut(s)	321, 453, 920, 2157
MaeII	ACGT	2 cut(s)	361, 1134
MaeIII	GTNAC	6 cut(s)	260, 761, 1037, 1135, 1718, 1946
MalI	GATC	9 cut(s)	15, 624, 1065, 1198, 1260, 1275, 1323, 1870, 1999
MboI	GATC	9 cut(s)	13, 622, 1063, 1196, 1258, 1273, 1321, 1868, 1997
MboII	GAAGA	9 cut(s)	177, 230, 602, 863, 898, 1178, 1292, 1634, 1848
MfeI	CAATTG	2 cut(s)	408, 661
MflI	RGATCY	4 cut(s)	1196, 1273, 1868, 1997
MhlI	GDGCHC	4 cut(s)	146, 477, 1014, 1612
MlsI	TGGCCA	2 cut(s)	870, 996
MluNI	TGGCCA	2 cut(s)	870, 996
MlyI	GAGTC	3 cut(s)	517, 658, 1492
MmeI	TCCRAC	2 cut(s)	1507, 2078
Mox20I	TGGCCA	2 cut(s)	870, 996
Mph1103I	ATGCAT	2 cut(s)	1558, 2133
MroNI	GCCGGC	1 cut(s)	154
MroXI	GAANNNNTTC	2 cut(s)	606, 756
MscI	TGGCCA	2 cut(s)	870, 996
MseI	TTAA	9 cut(s)	485, 507, 594, 767, 822, 948, 1020, 1248, 1380
MslI	CAYNNNNRTG	1 cut(s)	329
Msp20I	TGGCCA	2 cut(s)	870, 996
MspA1I	CMGCKG	2 cut(s)	1119, 1771
MspCI	CTTAAG	1 cut(s)	766
MspI	CCGG	4 cut(s)	155, 1386, 1865, 2135
MunI	CAATTG	2 cut(s)	408, 661
MvnI	CGCG	1 cut(s)	1119
NaeI	GCCGGC	1 cut(s)	156
NciI	CCSGG	3 cut(s)	1386, 1866, 2136
NcoI	CCATGG	1 cut(s)	871
NdeII	GATC	9 cut(s)	13, 622, 1063, 1196, 1258, 1273, 1321, 1868, 1997
NgoMIV	GCCGGC	1 cut(s)	154
NmuCI	GTSAC	5 cut(s)	260, 761, 1135, 1718, 1946
NsiI	ATGCAT	2 cut(s)	1558, 2133
NspI	RCATGY	1 cut(s)	2047
NspV	TTCGAA	2 cut(s)	349, 1058
OliI	CACNNNNGTG	1 cut(s)	329
PagI	TCATGA	1 cut(s)	1162
PaqCI	CACCTGC	1 cut(s)	406
PciSI	GCTCTTC	1 cut(s)	846
PdiI	GCCGGC	1 cut(s)	156
PdmI	GAANNNNTTC	2 cut(s)	606, 756
PfeI	GAWTC	6 cut(s)	346, 493, 607, 752, 1654, 1936
PflMI	CCANNNNNTGG	1 cut(s)	1475
PfoI	TCCNGGA	2 cut(s)	271, 1474
PleI	GAGTC	3 cut(s)	517, 657, 1492
PpsI	GAGTC	3 cut(s)	517, 657, 1492
PshAI	GACNNNNGTC	1 cut(s)	1365
PshBI	ATTAAT	1 cut(s)	507
PspEI	GGTNACC	2 cut(s)	1718, 1946
PspOMI	GGGCCC	1 cut(s)	142
PstI	CTGCAG	3 cut(s)	403, 1310, 1776
PsuI	RGATCY	4 cut(s)	1196, 1273, 1868, 1997
PvuII	CAGCTG	1 cut(s)	1771
RsaI	GTAC	3 cut(s)	8, 701, 1455
RsaNI	GTAC	3 cut(s)	7, 700, 1454
RseI	CAYNNNNRTG	1 cut(s)	329
SacII	CCGCGG	1 cut(s)	1120
SapI	GCTCTTC	1 cut(s)	846
SaqAI	TTAA	9 cut(s)	485, 507, 594, 767, 822, 948, 1020, 1248, 1380
Sau3AI	GATC	9 cut(s)	13, 622, 1063, 1196, 1258, 1273, 1321, 1868, 1997
ScaI	AGTACT	1 cut(s)	701
SchI	GAGTC	3 cut(s)	517, 658, 1492
SduI	GDGCHC	4 cut(s)	146, 477, 1014, 1612
SexAI	ACCWGGT	1 cut(s)	1624
SfcI	CTRYAG	4 cut(s)	399, 528, 1306, 1772
Sfr303I	CCGCGG	1 cut(s)	1120
SfuI	TTCGAA	2 cut(s)	349, 1058
SgrBI	CCGCGG	1 cut(s)	1120
SinI	GGWCC	5 cut(s)	1463, 1478, 1628, 2051, 2069
SmiMI	CAYNNNNRTG	1 cut(s)	329
SmlI	CTYRAG	1 cut(s)	766
SmoI	CTYRAG	1 cut(s)	766
SsiI	CCGC	9 cut(s)	116, 146, 149, 152, 687, 1117, 1119, 1724, 1823
SspI	AATATT	1 cut(s)	1172
SspMI	CTAG	4 cut(s)	321, 453, 920, 2157
StyI	CCWWGG	2 cut(s)	871, 1714
TaaI	ACNGT	6 cut(s)	266, 328, 382, 588, 820, 843
TaiI	ACGT	2 cut(s)	364, 1137
TaqI	TCGA	6 cut(s)	349, 1058, 1126, 1501, 1595, 1819
TatI	WGTACW	1 cut(s)	699
TauI	GCSGC	2 cut(s)	151, 154
TfiI	GAWTC	6 cut(s)	346, 493, 607, 752, 1654, 1936
Tru1I	TTAA	9 cut(s)	485, 507, 594, 767, 822, 948, 1020, 1248, 1380
Tru9I	TTAA	9 cut(s)	485, 507, 594, 767, 822, 948, 1020, 1248, 1380
TscAI	CASTG	6 cut(s)	331, 1186, 1315, 1369, 2020, 2071
TseFI	GTSAC	5 cut(s)	260, 761, 1135, 1718, 1946
Tsp45I	GTSAC	5 cut(s)	260, 761, 1135, 1718, 1946
TspGWI	ACGGA	2 cut(s)	1006, 1146
TspRI	CASTG	6 cut(s)	331, 1186, 1315, 1369, 2020, 2071
Van91I	CCANNNNNTGG	1 cut(s)	1475
Vha464I	CTTAAG	1 cut(s)	766
VneI	GTGCAC	1 cut(s)	473
VpaK11BI	GGWCC	5 cut(s)	1463, 1478, 1628, 2051, 2069
VspI	ATTAAT	1 cut(s)	507
XagI	CCTNNNNNAGG	1 cut(s)	2187
XapI	RAATTY	6 cut(s)	131, 566, 1242, 1376, 1920, 2121
XceI	RCATGY	1 cut(s)	2047
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1214
XmnI	GAANNNNTTC	2 cut(s)	606, 756
XspI	CTAG	4 cut(s)	321, 453, 920, 2157
ZrmI	AGTACT	1 cut(s)	701
Zsp2I	ATGCAT	2 cut(s)	1558, 2133

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.