Rmu_sc0001294.1_g000021

AKAP7 2'5' RNA ligase-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001294.1
OG0000535
Physical Location & Seq
Forward (+)
77486 .. 79464
1979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001294.1_g000021.1.cds

Sequence Viewer

Length: 549 bp
atgattagttaccacccaggtttctttctgattgttacatgtggattttcagaatgctcagtagtggagattacagataagctggaagaagaagtgcactcttgctcatctaccaagatttcaagtgctcaagatgacatagagatggcttcagcagttactgaagcaactaagttcacgacttgttcaagtgcatcgcaagataatgaagaagatgaagttttaggaggagaagcaaactccaacctggaaattactgattcttcccggggtgagaatctggacagtggttcgaatgaagatgattccgataatgaagatgaatatcaaaagttaaagaaaggatctgatgttgctgttgaacttgaagctgaatcatcaaagtcttctaccctatctggtatggaaataatccttatggatgaaaaaataatcattatgcgtaaatttattcagtatcagttttccttccatgaagctagtgaatgggtgactgaagttagttgttttccactttaccctataaagtgttatctcatcgattgctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AKAP7_NLS Auxin_canalis KH_1 PH_2

Protein Analysis

182

Amino Acids

20.19

Weight (kDa)

4.12

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 352
AcsI RAATTY 1 cut(s) 446
AcuI CTGAAG 3 cut(s) 135, 183, 516
AflIII ACRYGT 1 cut(s) 38
AgsI TTSAA 4 cut(s) 123, 189, 362, 368
AjnI CCWGG 2 cut(s) 16, 246
AluBI AGCT 3 cut(s) 82, 371, 479
AluI AGCT 3 cut(s) 82, 371, 479
Alw21I GWGCWC 2 cut(s) 99, 130
Alw44I GTGCAC 1 cut(s) 95
AlwI GGATC 1 cut(s) 352
AlwNI CAGNNNCTG 1 cut(s) 161
Ama87I CYCGRG 1 cut(s) 267
ApaLI GTGCAC 1 cut(s) 95
ApoI RAATTY 1 cut(s) 446
AsuC2I CCSGG 2 cut(s) 268, 269
AsuHPI GGTGA 2 cut(s) 284, 502
AsuII TTCGAA 1 cut(s) 293
AvaI CYCGRG 1 cut(s) 267
BaeGI GKGCMC 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 378
Bbv12I GWGCWC 2 cut(s) 99, 130
BccI CCATC 1 cut(s) 139
BciT130I CCWGG 2 cut(s) 18, 248
BcnI CCSGG 2 cut(s) 268, 269
BfaI CTAG 1 cut(s) 480
Bme1390I CCNGG 4 cut(s) 18, 248, 268, 269
BmeT110I CYCGRG 1 cut(s) 267
BmrFI CCNGG 4 cut(s) 18, 248, 268, 269
BmsI GCATC 1 cut(s) 203
BpiI GAAGAC 1 cut(s) 378
Bpu14I TTCGAA 1 cut(s) 293
BpuEI CTTGAG 1 cut(s) 114
BpuMI CCSGG 2 cut(s) 268, 269
Bsa29I ATCGAT 1 cut(s) 540
BsaBI GATNNNNATC 1 cut(s) 324
BsaJI CCNNGG 3 cut(s) 16, 267, 268
Bse8I GATNNNNATC 1 cut(s) 324
BseBI CCWGG 2 cut(s) 18, 248
BseCI ATCGAT 1 cut(s) 540
BseDI CCNNGG 3 cut(s) 16, 267, 268
BseGI GGATG 1 cut(s) 427
BseJI GATNNNNATC 1 cut(s) 324
BseMII CTCAG 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 243
BseSI GKGCMC 1 cut(s) 99
BshVI ATCGAT 1 cut(s) 540
BsiHKAI GWGCWC 2 cut(s) 99, 130
BsiHKCI CYCGRG 1 cut(s) 267
BsiSI CCGG 1 cut(s) 268
BsmI GAATGC 1 cut(s) 59
BsoBI CYCGRG 1 cut(s) 267
Bsp119I TTCGAA 1 cut(s) 293
Bsp1286I GDGCHC 2 cut(s) 99, 130
Bsp143I GATC 1 cut(s) 344
BspCNI CTCAG 1 cut(s) 71
BspDI ATCGAT 1 cut(s) 540
BspPI GGATC 1 cut(s) 352
BspT104I TTCGAA 1 cut(s) 293
BssECI CCNNGG 3 cut(s) 16, 267, 268
BssMI GATC 1 cut(s) 344
Bst2UI CCWGG 2 cut(s) 18, 248
Bst4CI ACNGT 1 cut(s) 287
BstBI TTCGAA 1 cut(s) 293
BstDEI CTNAG 2 cut(s) 58, 171
BstF5I GGATG 1 cut(s) 427
BstKTI GATC 1 cut(s) 347
BstMBI GATC 1 cut(s) 344
BstNI CCWGG 2 cut(s) 18, 248
BstNSI RCATGY 1 cut(s) 42
BstSCI CCNGG 4 cut(s) 16, 246, 266, 267
BstSLI GKGCMC 1 cut(s) 99
BstV2I GAAGAC 1 cut(s) 378
BstX2I RGATCY 1 cut(s) 344
BstYI RGATCY 1 cut(s) 344
Bsu15I ATCGAT 1 cut(s) 540
BsuTUI ATCGAT 1 cut(s) 540
BtgZI GCGATG 1 cut(s) 180
BtsCI GGATG 1 cut(s) 427
BtsIMutI CAGTG 1 cut(s) 292
CaiI CAGNNNCTG 1 cut(s) 161
Cfr9I CCCGGG 1 cut(s) 267
ClaI ATCGAT 1 cut(s) 540
CviAII CATG 2 cut(s) 39, 473
CviJI RGCY 4 cut(s) 82, 149, 371, 479
CviKI_1 RGCY 4 cut(s) 82, 149, 371, 479
DdeI CTNAG 2 cut(s) 58, 171
DpnI GATC 1 cut(s) 346
DpnII GATC 1 cut(s) 344
Eco57I CTGAAG 3 cut(s) 135, 183, 516
Eco88I CYCGRG 1 cut(s) 267
EcoRII CCWGG 2 cut(s) 16, 246
FaeI CATG 2 cut(s) 42, 476
FaiI YATR 7 cut(s) 40, 140, 404, 419, 440, 474, 524
FatI CATG 2 cut(s) 38, 472
FokI GGATG 1 cut(s) 434
FspBI CTAG 1 cut(s) 480
HapII CCGG 1 cut(s) 268
Hin1II CATG 2 cut(s) 42, 476
HinfI GANTC 4 cut(s) 260, 277, 305, 374
HpaII CCGG 1 cut(s) 268
HphI GGTGA 2 cut(s) 284, 502
Hpy166II GTNNAC 2 cut(s) 97, 177
Hpy188I TCNGA 4 cut(s) 30, 52, 310, 349
Hpy188III TCNNGA 3 cut(s) 131, 178, 281
Hpy8I GTNNAC 2 cut(s) 97, 177
HpyAV CCTTC 1 cut(s) 478
HpyCH4III ACNGT 1 cut(s) 287
HpyCH4V TGCA 2 cut(s) 97, 194
HpyF3I CTNAG 2 cut(s) 58, 171
Hsp92II CATG 2 cut(s) 42, 476
Kzo9I GATC 1 cut(s) 344
LpnPI CCDG 8 cut(s) 3, 30, 68, 233, 260, 266, 281, 384
LweI GCATC 1 cut(s) 203
MaeI CTAG 1 cut(s) 480
MaeIII GTNAC 4 cut(s) 8, 34, 157, 490
MalI GATC 1 cut(s) 346
MboI GATC 1 cut(s) 344
MboII GAAGA 8 cut(s) 98, 101, 221, 224, 255, 311, 329, 378
MflI RGATCY 1 cut(s) 344
MhlI GDGCHC 2 cut(s) 99, 130
MluCI AATT 2 cut(s) 252, 446
MmeI TCCRAC 1 cut(s) 267
MnlI CCTC 1 cut(s) 221
MseI TTAA 1 cut(s) 335
MslI CAYNNNNRTG 1 cut(s) 143
MspI CCGG 1 cut(s) 268
MspR9I CCNGG 4 cut(s) 18, 248, 268, 269
Mva1269I GAATGC 1 cut(s) 59
MvaI CCWGG 2 cut(s) 18, 248
NciI CCSGG 2 cut(s) 268, 269
NdeII GATC 1 cut(s) 344
NlaIII CATG 2 cut(s) 42, 476
NmuCI GTSAC 1 cut(s) 490
NspI RCATGY 1 cut(s) 42
NspV TTCGAA 1 cut(s) 293
PciI ACATGT 1 cut(s) 38
PctI GAATGC 1 cut(s) 59
PfeI GAWTC 4 cut(s) 260, 277, 305, 374
PscI ACATGT 1 cut(s) 38
Psp6I CCWGG 2 cut(s) 16, 246
PspGI CCWGG 2 cut(s) 16, 246
PstNI CAGNNNCTG 1 cut(s) 161
PsuI RGATCY 1 cut(s) 344
RseI CAYNNNNRTG 1 cut(s) 143
SaqAI TTAA 1 cut(s) 335
Sau3AI GATC 1 cut(s) 344
ScrFI CCNGG 4 cut(s) 18, 248, 268, 269
SduI GDGCHC 2 cut(s) 99, 130
SetI ASST 5 cut(s) 22, 84, 249, 373, 481
SfaNI GCATC 1 cut(s) 203
SfuI TTCGAA 1 cut(s) 293
SmaI CCCGGG 1 cut(s) 269
SmiMI CAYNNNNRTG 1 cut(s) 143
SmlI CTYRAG 1 cut(s) 129
SmoI CTYRAG 1 cut(s) 129
Sse9I AATT 2 cut(s) 252, 446
SspMI CTAG 1 cut(s) 480
StyD4I CCNGG 4 cut(s) 16, 246, 266, 267
TaaI ACNGT 1 cut(s) 287
TaqI TCGA 2 cut(s) 293, 540
TasI AATT 2 cut(s) 252, 446
TfiI GAWTC 4 cut(s) 260, 277, 305, 374
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TscAI CASTG 1 cut(s) 292
TseFI GTSAC 1 cut(s) 490
Tsp45I GTSAC 1 cut(s) 490
TspDTI ATGAA 7 cut(s) 222, 231, 312, 330, 336, 438, 489
TspMI CCCGGG 1 cut(s) 267
TspRI CASTG 1 cut(s) 292
VneI GTGCAC 1 cut(s) 95
XapI RAATTY 1 cut(s) 446
XceI RCATGY 1 cut(s) 42
XmaI CCCGGG 1 cut(s) 267
XspI CTAG 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.