Rh5BG192200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000535
Physical Location & Seq
Forward (+)
21205664 .. 21217140
11477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG192200.1

Sequence Viewer

Length: 894 bp
ATGGCTCTTGCCACAATTTTTCCTTCCTCCAGCGTGTCCTATGTTGTTCTTGGGCGCGCTGCTGAATGCTATTCTTTATTTTTCCTCCTCCTTATGCTCGGTGTTTGCGGCTTTGATGGGGGACTATTAGATCCGGTTTTGGATCGGACTTTGGATCCTTATGTGTTGGGAGAAAGAGGTACTGAGAATAGCATAATCGATCCTTGGAGGTGGAGAGAGTTTCTGGGGGCGGAGGCTCTGATGCGTCTTGACGAATTCTATTCGGGGCGTTTTGGCCTTGTTGGTTTTGGAGATGAGGGCAGCAATCTGGTAATGGTGAAGGATGGTGATCTGGCTCGATTTGCTTTCTTTGGTGATGCTTGCGACTGGGGTGGGAATCAACCTAGTCGTGGTGGTCTAGCAGTGGCTCTGTGCAGAAGCTTTGCGGCGGCGTGGCAAGTCGGAGGTGGTGGTGGGATGGATGCTAGGGTGTCCTCATCAATTAGCACCATCTGGGCCGTGTTGGGCCAGAACTATAACCCCAACATTTGGCTTGATGTGGAGTTGGTTGCCCTTATGTTGTTGGCTTCCCTTGTGTTGCGGGGTTGCTGGAGTAGGGTATTTTGGGCCCACTGTGTTGAATCTTCTAGTGAACTTGTTGCTGCTGAAGCATCCAAAAAGATTGAGATTGCAGGCTTGGAGGACTCGGAGCATGAAGAAGGAATGGAAGCCCTCCACTGCATGCGCTTTGCTTACGAAACCAGCCTGGTCATTTTGCTGACGTCCTCATCCCTGACGTGTCTCCACTCTCCAACCCAGAGATTCTCGACGTCCTCATCCTCACTCTTTAGATGCTTCGTGTTTATCGAGGAATGGGATTGTGTTTGGATCAATATCTGCTTTGTGATTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

297

Amino Acids

32.62

Weight (kDa)

4.57

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 764, 812
AccB7I CCANNNNNTGG 1 cut(s) 528
AccII CGCG 1 cut(s) 57
AciI CCGC 5 cut(s) 108, 230, 425, 428, 580
AclWI GGATC 6 cut(s) 125, 149, 150, 162, 194, 875
AcsI RAATTY 1 cut(s) 254
AcuI CTGAAG 1 cut(s) 666
AcyI GRCGYC 2 cut(s) 761, 809
AfaI GTAC 1 cut(s) 181
AfiI CCNNNNNNNGG 2 cut(s) 389, 528
AflIII ACRYGT 1 cut(s) 776
AgsI TTSAA 1 cut(s) 620
AjiI CACGTC 1 cut(s) 777
AjnI CCWGG 1 cut(s) 744
AluBI AGCT 1 cut(s) 420
AluI AGCT 1 cut(s) 420
Alw26I GTCTC 1 cut(s) 785
AlwI GGATC 6 cut(s) 125, 149, 150, 162, 194, 875
AoxI GGCC 4 cut(s) 274, 495, 505, 606
ApaI GGGCCC 1 cut(s) 610
ApeKI GCWGC 3 cut(s) 59, 300, 641
ApoI RAATTY 1 cut(s) 254
AspLEI GCGC 3 cut(s) 57, 59, 726
AspS9I GGNCC 4 cut(s) 495, 505, 606, 607
AsuHPI GGTGA 3 cut(s) 328, 338, 365
BaeGI GKGCMC 1 cut(s) 610
BamHI GGATCC 1 cut(s) 154
BanII GRGCYC 1 cut(s) 610
BbvI GCAGC 3 cut(s) 46, 312, 628
BccI CCATC 4 cut(s) 110, 317, 451, 497
BceAI ACGGC 1 cut(s) 482
BciT130I CCWGG 1 cut(s) 746
BcoDI GTCTC 1 cut(s) 785
BfaI CTAG 4 cut(s) 384, 398, 465, 627
BisI GCNGC 6 cut(s) 60, 109, 301, 426, 429, 642
BlsI GCNGC 6 cut(s) 61, 110, 302, 427, 430, 643
Bme1390I CCNGG 1 cut(s) 746
BmgBI CACGTC 1 cut(s) 777
BmgT120I GGNCC 4 cut(s) 495, 505, 606, 607
BmiI GGNNCC 2 cut(s) 156, 608
BmrFI CCNGG 1 cut(s) 746
BmrI ACTGGG 1 cut(s) 376
BmsI GCATC 5 cut(s) 231, 346, 451, 659, 821
BmuI ACTGGG 1 cut(s) 376
BpmI CTGGAG 2 cut(s) 13, 610
Bsa29I ATCGAT 1 cut(s) 198
BsaBI GATNNNNATC 2 cut(s) 327, 872
BsaHI GRCGYC 2 cut(s) 761, 809
BsaJI CCNNGG 1 cut(s) 203
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 2 cut(s) 389, 528
Bse1I ACTGG 1 cut(s) 371
Bse8I GATNNNNATC 2 cut(s) 327, 872
BseBI CCWGG 1 cut(s) 746
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 203
BseGI GGATG 6 cut(s) 328, 462, 466, 650, 767, 815
BseJI GATNNNNATC 2 cut(s) 327, 872
BseLI CCNNNNNNNGG 2 cut(s) 389, 528
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 1 cut(s) 371
BsePI GCGCGC 1 cut(s) 55
BseRI GAGGAG 1 cut(s) 77
BseSI GKGCMC 1 cut(s) 610
BseXI GCAGC 3 cut(s) 46, 312, 628
BsgI GTGCAG 1 cut(s) 433
Bsh1236I CGCG 1 cut(s) 57
BshFI GGCC 4 cut(s) 276, 497, 507, 608
BshVI ATCGAT 1 cut(s) 198
BsiSI CCGG 1 cut(s) 134
BslFI GGGAC 1 cut(s) 135
BslI CCNNNNNNNGG 2 cut(s) 389, 528
BsmAI GTCTC 1 cut(s) 785
BsmFI GGGAC 1 cut(s) 135
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 4 cut(s) 276, 497, 507, 608
Bsp120I GGGCCC 1 cut(s) 606
Bsp1286I GDGCHC 1 cut(s) 610
Bsp143I GATC 6 cut(s) 130, 142, 154, 199, 328, 867
BspACI CCGC 5 cut(s) 108, 230, 425, 428, 580
BspANI GGCC 4 cut(s) 276, 497, 507, 608
BspCNI CTCAG 1 cut(s) 175
BspDI ATCGAT 1 cut(s) 198
BspFNI CGCG 1 cut(s) 57
BspLI GGNNCC 2 cut(s) 156, 608
BspPI GGATC 6 cut(s) 125, 149, 150, 162, 194, 875
BsrI ACTGG 1 cut(s) 371
BssECI CCNNGG 1 cut(s) 203
BssHII GCGCGC 1 cut(s) 55
BssMI GATC 6 cut(s) 130, 142, 154, 199, 328, 867
BssNI GRCGYC 2 cut(s) 761, 809
BssT1I CCWWGG 1 cut(s) 203
Bst2UI CCWGG 1 cut(s) 746
Bst4CI ACNGT 1 cut(s) 614
BstACI GRCGYC 2 cut(s) 761, 809
BstC8I GCNNGC 4 cut(s) 57, 361, 673, 722
BstDEI CTNAG 1 cut(s) 183
BstF5I GGATG 6 cut(s) 328, 462, 466, 650, 767, 815
BstFNI CGCG 1 cut(s) 57
BstHHI GCGC 3 cut(s) 57, 59, 726
BstKTI GATC 6 cut(s) 133, 145, 157, 202, 331, 870
BstMAI GTCTC 1 cut(s) 785
BstMBI GATC 6 cut(s) 130, 142, 154, 199, 328, 867
BstMWI GCNNNNNNNGC 2 cut(s) 341, 647
BstNI CCWGG 1 cut(s) 746
BstNSI RCATGY 1 cut(s) 724
BstSCI CCNGG 1 cut(s) 744
BstSLI GKGCMC 1 cut(s) 610
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 3 cut(s) 46, 312, 628
BstX2I RGATCY 2 cut(s) 130, 154
BstYI RGATCY 2 cut(s) 130, 154
Bsu15I ATCGAT 1 cut(s) 198
BsuRI GGCC 4 cut(s) 276, 497, 507, 608
BsuTUI ATCGAT 1 cut(s) 198
BtrI CACGTC 1 cut(s) 777
BtsCI GGATG 6 cut(s) 328, 462, 466, 650, 767, 815
BtsI GCAGTG 2 cut(s) 408, 715
BtsIMutI CAGTG 3 cut(s) 408, 610, 715
Cac8I GCNNGC 4 cut(s) 57, 361, 673, 722
CfoI GCGC 3 cut(s) 57, 59, 726
Cfr13I GGNCC 4 cut(s) 495, 505, 606, 607
ClaI ATCGAT 1 cut(s) 198
CseI GACGC 1 cut(s) 233
Csp6I GTAC 1 cut(s) 180
CviAII CATG 2 cut(s) 692, 721
CviQI GTAC 1 cut(s) 180
DdeI CTNAG 1 cut(s) 183
DpnI GATC 6 cut(s) 132, 144, 156, 201, 330, 869
DpnII GATC 6 cut(s) 130, 142, 154, 199, 328, 867
EciI GGCGGA 1 cut(s) 245
Eco130I CCWWGG 1 cut(s) 203
Eco24I GRGCYC 1 cut(s) 610
Eco57I CTGAAG 1 cut(s) 666
EcoRI GAATTC 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 744
EcoT14I CCWWGG 1 cut(s) 203
EcoT38I GRGCYC 1 cut(s) 610
ErhI CCWWGG 1 cut(s) 203
FaeI CATG 2 cut(s) 695, 724
FaiI YATR 8 cut(s) 42, 95, 162, 194, 516, 557, 693, 722
FaqI GGGAC 1 cut(s) 135
FatI CATG 2 cut(s) 691, 720
FauI CCCGC 1 cut(s) 573
Fnu4HI GCNGC 6 cut(s) 60, 109, 301, 426, 429, 642
FokI GGATG 6 cut(s) 335, 469, 473, 637, 754, 802
FriOI GRGCYC 1 cut(s) 610
Fsp4HI GCNGC 6 cut(s) 60, 109, 301, 426, 429, 642
FspBI CTAG 4 cut(s) 384, 398, 465, 627
GlaI GCGC 3 cut(s) 56, 58, 725
GluI GCNGC 6 cut(s) 60, 109, 301, 426, 429, 642
GsuI CTGGAG 2 cut(s) 13, 610
HaeIII GGCC 4 cut(s) 276, 497, 507, 608
HapII CCGG 1 cut(s) 134
HgaI GACGC 1 cut(s) 233
HhaI GCGC 3 cut(s) 57, 59, 726
Hin1I GRCGYC 2 cut(s) 761, 809
Hin1II CATG 2 cut(s) 695, 724
Hin6I GCGC 3 cut(s) 55, 57, 724
HinP1I GCGC 3 cut(s) 55, 57, 724
HindIII AAGCTT 1 cut(s) 418
HinfI GANTC 4 cut(s) 376, 620, 683, 801
HpaII CCGG 1 cut(s) 134
HphI GGTGA 3 cut(s) 328, 338, 365
Hpy166II GTNNAC 1 cut(s) 632
Hpy188I TCNGA 4 cut(s) 147, 240, 443, 688
Hpy188III TCNNGA 2 cut(s) 248, 805
Hpy8I GTNNAC 1 cut(s) 632
Hpy99I CGWCG 1 cut(s) 811
HpyAV CCTTC 3 cut(s) 33, 313, 692
HpyCH4III ACNGT 1 cut(s) 614
HpyCH4IV ACGT 3 cut(s) 761, 776, 809
HpyCH4V TGCA 3 cut(s) 414, 671, 720
HpyF10VI GCNNNNNNNGC 2 cut(s) 341, 647
HpyF3I CTNAG 1 cut(s) 183
HpySE526I ACGT 3 cut(s) 761, 776, 809
Hsp92I GRCGYC 2 cut(s) 761, 809
Hsp92II CATG 2 cut(s) 695, 724
HspAI GCGC 3 cut(s) 55, 57, 724
Kzo9I GATC 6 cut(s) 130, 142, 154, 199, 328, 867
LmnI GCTCC 1 cut(s) 688
Lsp1109I GCAGC 3 cut(s) 46, 312, 628
LweI GCATC 5 cut(s) 231, 346, 451, 659, 821
MaeI CTAG 4 cut(s) 384, 398, 465, 627
MaeII ACGT 3 cut(s) 761, 776, 809
MalI GATC 6 cut(s) 132, 144, 156, 201, 330, 869
MboI GATC 6 cut(s) 130, 142, 154, 199, 328, 867
MboII GAAGA 2 cut(s) 615, 707
MflI RGATCY 2 cut(s) 130, 154
MhlI GDGCHC 1 cut(s) 610
MluCI AATT 3 cut(s) 15, 254, 480
MlyI GAGTC 1 cut(s) 677
MmeI TCCRAC 2 cut(s) 421, 815
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 1 cut(s) 746
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 1 cut(s) 746
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 2 cut(s) 341, 647
NdeII GATC 6 cut(s) 130, 142, 154, 199, 328, 867
NlaIII CATG 2 cut(s) 695, 724
NlaIV GGNNCC 2 cut(s) 156, 608
NspI RCATGY 1 cut(s) 724
PaeI GCATGC 1 cut(s) 724
PauI GCGCGC 1 cut(s) 55
PcsI WCGNNNNNNNCGW 1 cut(s) 843
PctI GAATGC 1 cut(s) 71
PfeI GAWTC 3 cut(s) 376, 620, 801
PflMI CCANNNNNTGG 1 cut(s) 528
PkrI GCNGC 6 cut(s) 61, 110, 302, 427, 430, 643
PleI GAGTC 1 cut(s) 677
PpsI GAGTC 1 cut(s) 677
Psp6I CCWGG 1 cut(s) 744
PspGI CCWGG 1 cut(s) 744
PspN4I GGNNCC 2 cut(s) 156, 608
PspOMI GGGCCC 1 cut(s) 606
PspPI GGNCC 4 cut(s) 495, 505, 606, 607
PsuI RGATCY 2 cut(s) 130, 154
PteI GCGCGC 1 cut(s) 55
RsaI GTAC 1 cut(s) 181
RsaNI GTAC 1 cut(s) 180
SatI GCNGC 6 cut(s) 60, 109, 301, 426, 429, 642
Sau3AI GATC 6 cut(s) 130, 142, 154, 199, 328, 867
Sau96I GGNCC 4 cut(s) 495, 505, 606, 607
SchI GAGTC 1 cut(s) 677
ScrFI CCNGG 1 cut(s) 746
SduI GDGCHC 1 cut(s) 610
SetI ASST 8 cut(s) 181, 212, 385, 422, 448, 764, 779, 812
SfaNI GCATC 5 cut(s) 231, 346, 451, 659, 821
SphI GCATGC 1 cut(s) 724
Sse9I AATT 3 cut(s) 15, 254, 480
SsiI CCGC 5 cut(s) 108, 230, 425, 428, 580
SspMI CTAG 4 cut(s) 384, 398, 465, 627
StyD4I CCNGG 1 cut(s) 744
StyI CCWWGG 1 cut(s) 203
TaaI ACNGT 1 cut(s) 614
TaiI ACGT 3 cut(s) 764, 779, 812
TaqI TCGA 4 cut(s) 198, 337, 806, 846
TasI AATT 3 cut(s) 15, 254, 480
TauI GCSGC 3 cut(s) 111, 428, 431
TfiI GAWTC 3 cut(s) 376, 620, 801
TscAI CASTG 3 cut(s) 408, 617, 722
TseI GCWGC 3 cut(s) 59, 300, 641
TspDTI ATGAA 1 cut(s) 708
TspRI CASTG 3 cut(s) 408, 617, 722
Van91I CCANNNNNTGG 1 cut(s) 528
XapI RAATTY 1 cut(s) 254
XceI RCATGY 1 cut(s) 724
XspI CTAG 4 cut(s) 384, 398, 465, 627
ZraI GACGTC 2 cut(s) 762, 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.