Rh2AG221500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000535
Physical Location & Seq
Reverse (-)
23205171 .. 23205774
604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG221500.1

Sequence Viewer

Length: 525 bp
ATGGCTATTGCCACAAATTTCCCCTTCTCCGGCGTGTCATGCTTTGCTTTAGGGTGCGTTGCAGGTCGTTTCTCTTTGCTTTTCCTTCTCCTTGGATTCGCTGTTAGCGGTTTTGATGGAGGACTTTTTTATCCGGGATTGGCTCTTTCTTTGATTCCTGATGGGTCGGGTGATGGAGGTTCTGGGGATAACGCAATTGATCCTTGGAGGTGGAGGGGTTTTCTACCCTCTGGTATTCGCGTCAATCGCAGCCTAACAACCTGCTTGAACCTAGTGCATCATCGAGGGCCAGTCGCGGCTGCTGCTGCTGGAGTTGATGGCGGCAATCTGGTGTTGGTGAAGGACGGCGCTCTGGCTTTGTCTGGGAGTGGTCTGTGTCAGCTTGGTCATGGTGGCTTGTTGGTGGAATGGTACCAGTGCCTTACGGCGGCGGCGCGGGACGGAGGTGGTAGTTATGCAGGTTTCTTTGGGTGTGGGCTGGCCCTTTGCTTTGGGGCTGCTGGATTAGGATGTCTTGGGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

17.42

Weight (kDa)

6.23

Isoelectric Point (pI)

26.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 53, 269, 449
Acc65I GGTACC 1 cut(s) 411
AccB1I GGYRCC 1 cut(s) 411
AccII CGCG 3 cut(s) 240, 296, 436
AciI CCGC 6 cut(s) 108, 296, 321, 428, 431, 436
AclWI GGATC 1 cut(s) 194
AcsI RAATTY 1 cut(s) 16
AfaI GTAC 1 cut(s) 413
AfiI CCNNNNNNNGG 2 cut(s) 29, 427
AgsI TTSAA 1 cut(s) 268
AluBI AGCT 1 cut(s) 382
AluI AGCT 1 cut(s) 382
AlwI GGATC 1 cut(s) 194
AoxI GGCC 3 cut(s) 287, 480, 518
ApaI GGGCCC 1 cut(s) 522
ApeKI GCWGC 5 cut(s) 249, 299, 302, 305, 497
ApoI RAATTY 1 cut(s) 16
Asp718I GGTACC 1 cut(s) 411
AspLEI GCGC 2 cut(s) 350, 436
AspS9I GGNCC 4 cut(s) 287, 481, 518, 519
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 2 cut(s) 182, 349
BaeGI GKGCMC 1 cut(s) 522
BanI GGYRCC 1 cut(s) 411
BanII GRGCYC 1 cut(s) 522
BbvI GCAGC 5 cut(s) 261, 286, 289, 292, 484
BccI CCATC 4 cut(s) 110, 155, 167, 311
BceAI ACGGC 2 cut(s) 361, 441
BcnI CCSGG 1 cut(s) 135
BfaI CTAG 1 cut(s) 272
BfoI RGCGCY 1 cut(s) 351
BfuAI ACCTGC 3 cut(s) 53, 269, 449
BisI GCNGC 9 cut(s) 250, 297, 300, 303, 306, 322, 429, 432, 498
BlsI GCNGC 9 cut(s) 251, 298, 301, 304, 307, 323, 430, 433, 499
Bme1390I CCNGG 1 cut(s) 135
BmgT120I GGNCC 4 cut(s) 287, 481, 518, 519
BmiI GGNNCC 2 cut(s) 413, 520
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 286
BpmI CTGGAG 1 cut(s) 330
BpuMI CCSGG 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 91, 203
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 427
Bse1I ACTGG 2 cut(s) 290, 415
BseDI CCNNGG 2 cut(s) 91, 203
BseGI GGATG 1 cut(s) 515
BseLI CCNNNNNNNGG 2 cut(s) 29, 427
BseNI ACTGG 2 cut(s) 290, 415
BseSI GKGCMC 1 cut(s) 522
BseXI GCAGC 5 cut(s) 261, 286, 289, 292, 484
Bsh1236I CGCG 3 cut(s) 240, 296, 436
BshFI GGCC 3 cut(s) 289, 482, 520
BshNI GGYRCC 1 cut(s) 411
BsiSI CCGG 2 cut(s) 30, 134
BslFI GGGAC 1 cut(s) 452
BslI CCNNNNNNNGG 2 cut(s) 29, 427
BsmFI GGGAC 1 cut(s) 452
BsnI GGCC 3 cut(s) 289, 482, 520
Bsp120I GGGCCC 1 cut(s) 518
Bsp1286I GDGCHC 1 cut(s) 522
Bsp143I GATC 1 cut(s) 199
BspACI CCGC 6 cut(s) 108, 296, 321, 428, 431, 436
BspANI GGCC 3 cut(s) 289, 482, 520
BspFNI CGCG 3 cut(s) 240, 296, 436
BspLI GGNNCC 2 cut(s) 413, 520
BspMI ACCTGC 3 cut(s) 53, 269, 449
BspPI GGATC 1 cut(s) 194
BspT107I GGYRCC 1 cut(s) 411
BsrI ACTGG 2 cut(s) 290, 415
BssECI CCNNGG 2 cut(s) 91, 203
BssMI GATC 1 cut(s) 199
BssT1I CCWWGG 2 cut(s) 91, 203
BstC8I GCNNGC 1 cut(s) 480
BstF5I GGATG 1 cut(s) 515
BstFNI CGCG 3 cut(s) 240, 296, 436
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 2 cut(s) 350, 436
BstKTI GATC 1 cut(s) 202
BstMBI GATC 1 cut(s) 199
BstMWI GCNNNNNNNGC 4 cut(s) 39, 246, 302, 305
BstSCI CCNGG 1 cut(s) 133
BstSLI GKGCMC 1 cut(s) 522
BstUI CGCG 3 cut(s) 240, 296, 436
BstV1I GCAGC 5 cut(s) 261, 286, 289, 292, 484
BsuRI GGCC 3 cut(s) 289, 482, 520
BtsCI GGATG 1 cut(s) 515
BtsIMutI CAGTG 1 cut(s) 422
BveI ACCTGC 3 cut(s) 53, 269, 449
Cac8I GCNNGC 1 cut(s) 480
CfoI GCGC 2 cut(s) 350, 436
Cfr13I GGNCC 4 cut(s) 287, 481, 518, 519
CseI GACGC 1 cut(s) 229
Csp6I GTAC 1 cut(s) 412
CviAII CATG 2 cut(s) 39, 389
CviQI GTAC 1 cut(s) 412
DpnI GATC 1 cut(s) 201
DpnII GATC 1 cut(s) 199
Eco130I CCWWGG 2 cut(s) 91, 203
Eco24I GRGCYC 1 cut(s) 522
EcoO109I RGGNCCY 1 cut(s) 519
EcoT14I CCWWGG 2 cut(s) 91, 203
EcoT38I GRGCYC 1 cut(s) 522
ErhI CCWWGG 2 cut(s) 91, 203
FaeI CATG 2 cut(s) 42, 392
FaiI YATR 3 cut(s) 40, 390, 456
FaqI GGGAC 1 cut(s) 452
FatI CATG 2 cut(s) 38, 388
FauI CCCGC 1 cut(s) 429
Fnu4HI GCNGC 9 cut(s) 250, 297, 300, 303, 306, 322, 429, 432, 498
FriOI GRGCYC 1 cut(s) 522
Fsp4HI GCNGC 9 cut(s) 250, 297, 300, 303, 306, 322, 429, 432, 498
FspBI CTAG 1 cut(s) 272
GlaI GCGC 2 cut(s) 349, 435
GluI GCNGC 9 cut(s) 250, 297, 300, 303, 306, 322, 429, 432, 498
GsuI CTGGAG 1 cut(s) 330
HaeII RGCGCY 1 cut(s) 351
HaeIII GGCC 3 cut(s) 289, 482, 520
HapII CCGG 2 cut(s) 30, 134
HgaI GACGC 1 cut(s) 229
HhaI GCGC 2 cut(s) 350, 436
Hin1II CATG 2 cut(s) 42, 392
Hin6I GCGC 2 cut(s) 348, 434
HinP1I GCGC 2 cut(s) 348, 434
HinfI GANTC 2 cut(s) 96, 154
HpaII CCGG 2 cut(s) 30, 134
HphI GGTGA 2 cut(s) 182, 349
Hpy188III TCNNGA 1 cut(s) 158
HpyAV CCTTC 3 cut(s) 34, 95, 334
HpyCH4V TGCA 3 cut(s) 62, 277, 458
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 246, 302, 305
Hsp92II CATG 2 cut(s) 42, 392
HspAI GCGC 2 cut(s) 348, 434
KpnI GGTACC 1 cut(s) 415
Kzo9I GATC 1 cut(s) 199
Lsp1109I GCAGC 5 cut(s) 261, 286, 289, 292, 484
LweI GCATC 1 cut(s) 286
MaeI CTAG 1 cut(s) 272
MalI GATC 1 cut(s) 201
MboI GATC 1 cut(s) 199
MfeI CAATTG 1 cut(s) 195
MhlI GDGCHC 1 cut(s) 522
MluCI AATT 2 cut(s) 16, 195
MnlI CCTC 7 cut(s) 113, 170, 201, 207, 238, 278, 437
MspI CCGG 2 cut(s) 30, 134
MspR9I CCNGG 1 cut(s) 135
MunI CAATTG 1 cut(s) 195
MvnI CGCG 3 cut(s) 240, 296, 436
MwoI GCNNNNNNNGC 4 cut(s) 39, 246, 302, 305
NciI CCSGG 1 cut(s) 135
NdeII GATC 1 cut(s) 199
NlaIII CATG 2 cut(s) 42, 392
NlaIV GGNNCC 2 cut(s) 413, 520
PfeI GAWTC 2 cut(s) 96, 154
PfoI TCCNGGA 1 cut(s) 133
PkrI GCNGC 9 cut(s) 251, 298, 301, 304, 307, 323, 430, 433, 499
PspN4I GGNNCC 2 cut(s) 413, 520
PspOMI GGGCCC 1 cut(s) 518
PspPI GGNCC 4 cut(s) 287, 481, 518, 519
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
SatI GCNGC 9 cut(s) 250, 297, 300, 303, 306, 322, 429, 432, 498
Sau3AI GATC 1 cut(s) 199
Sau96I GGNCC 4 cut(s) 287, 481, 518, 519
ScrFI CCNGG 1 cut(s) 135
SduI GDGCHC 1 cut(s) 522
SetI ASST 8 cut(s) 67, 181, 212, 263, 273, 384, 448, 463
SfaNI GCATC 1 cut(s) 286
Sse9I AATT 2 cut(s) 16, 195
SsiI CCGC 6 cut(s) 108, 296, 321, 428, 431, 436
SspMI CTAG 1 cut(s) 272
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 2 cut(s) 91, 203
TaqI TCGA 1 cut(s) 283
TasI AATT 2 cut(s) 16, 195
TauI GCSGC 4 cut(s) 299, 324, 431, 434
TfiI GAWTC 2 cut(s) 96, 154
TscAI CASTG 1 cut(s) 422
TseI GCWGC 5 cut(s) 249, 299, 302, 305, 497
TspGWI ACGGA 1 cut(s) 456
TspRI CASTG 1 cut(s) 422
XapI RAATTY 1 cut(s) 16
XspI CTAG 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.