Rh5AG140800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000535
Physical Location & Seq
Reverse (-)
14607434 .. 14608177
744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG140800.1

Sequence Viewer

Length: 576 bp
ATGGCCGGCAACGGTGAAATTCTAACTAAGGGGGTGAGGCTCTCTCCTTTCTCTCTGATCGACATGGCTGCTGGGCAGCAAAATTTCCCGACGTCGGTTGGGATTGAAGATGGTTTGGAGGTCGATTTTGCTACTGTGGTTGACGAAGACTCTCGAGCCTGGGTTGTTGTTGGGATCCGATTTCTGCTGGGATCAGGATTGGGTCGGCAATCTTTGTTTGTCTTTATGGATCGAGGTTCTGGAAAGGCCGCCGGTGATCTGGTATTTTTGGTGGCGGCGCCTTCCACGGTGACGGTGGTAGCGGCGTCTATTGTGGTGGGTCAATGGTGCATTTCGACGACGACCGGAAAGGGCATGGGTGATGGTCCGATCGGCCTTGCCTTGGCGAAGATGCAGAGGTACTTTTCCGTTGGGCGGCGTTACAGATCTGGAAGGGTGGCCGGACGTTGGGCTGCGGCTGCTGCTGTTGGAGTTGATGGCAGGTCCTTTACGGCGGCGGCGCGGGACGGAGGTGGTGGTTATGCAGATGATGGAGTTGAGGGTGATTTTTCATCCTCTTCTCCTAGTACTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

19.61

Weight (kDa)

5.42

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 95
Acc36I ACCTGC 1 cut(s) 471
AccB1I GGYRCC 1 cut(s) 277
AccII CGCG 1 cut(s) 502
AciI CCGC 8 cut(s) 249, 275, 302, 415, 455, 494, 497, 502
AclWI GGATC 4 cut(s) 169, 182, 199, 237
AcoI YGGCCR 2 cut(s) 3, 438
AcsI RAATTY 2 cut(s) 18, 82
AcyI GRCGYC 3 cut(s) 92, 278, 305
AfaI GTAC 2 cut(s) 401, 568
AfiI CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
AgsI TTSAA 1 cut(s) 107
AjnI CCWGG 1 cut(s) 158
AlwI GGATC 4 cut(s) 169, 182, 199, 237
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 4 cut(s) 3, 246, 373, 438
ApeKI GCWGC 5 cut(s) 68, 76, 452, 458, 461
ApoI RAATTY 2 cut(s) 18, 82
AspLEI GCGC 2 cut(s) 280, 502
AspS9I GGNCC 2 cut(s) 365, 483
AsuHPI GGTGA 6 cut(s) 26, 46, 266, 301, 371, 554
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 2 cut(s) 365, 483
BamHI GGATCC 1 cut(s) 174
BanI GGYRCC 1 cut(s) 277
BbsI GAAGAC 1 cut(s) 153
BbvI GCAGC 5 cut(s) 55, 88, 439, 445, 448
BccI CCATC 4 cut(s) 104, 356, 470, 524
BceAI ACGGC 1 cut(s) 507
BcgI CGANNNNNNTGC 2 cut(s) 50, 84
BciT130I CCWGG 1 cut(s) 160
BfaI CTAG 1 cut(s) 564
BfoI RGCGCY 1 cut(s) 281
BfuAI ACCTGC 1 cut(s) 471
BglII AGATCT 1 cut(s) 425
BmcAI AGTACT 1 cut(s) 568
Bme1390I CCNGG 1 cut(s) 160
Bme18I GGWCC 2 cut(s) 365, 483
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 2 cut(s) 365, 483
BmiI GGNNCC 2 cut(s) 176, 279
BmrFI CCNGG 1 cut(s) 160
BmsI GCATC 1 cut(s) 381
BpiI GAAGAC 1 cut(s) 153
BplI GAGNNNNNCTC 2 cut(s) 28, 60
BsaHI GRCGYC 3 cut(s) 92, 278, 305
BsaJI CCNNGG 3 cut(s) 159, 285, 381
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
Bse118I RCCGGY 2 cut(s) 5, 251
BseBI CCWGG 1 cut(s) 160
BseDI CCNNGG 3 cut(s) 159, 285, 381
BseGI GGATG 1 cut(s) 551
BseLI CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
BseXI GCAGC 5 cut(s) 55, 88, 439, 445, 448
BseYI CCCAGC 2 cut(s) 71, 187
Bsh1236I CGCG 1 cut(s) 502
Bsh1285I CGRYCG 2 cut(s) 345, 372
BshFI GGCC 4 cut(s) 5, 248, 375, 440
BshNI GGYRCC 1 cut(s) 277
BsiEI CGRYCG 2 cut(s) 345, 372
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 4 cut(s) 6, 252, 345, 441
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
BsmFI GGGAC 1 cut(s) 518
BsnI GGCC 4 cut(s) 5, 248, 375, 440
BsoBI CYCGRG 1 cut(s) 153
Bsp143I GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
BspACI CCGC 8 cut(s) 249, 275, 302, 415, 455, 494, 497, 502
BspANI GGCC 4 cut(s) 5, 248, 375, 440
BspFNI CGCG 1 cut(s) 502
BspLI GGNNCC 2 cut(s) 176, 279
BspMI ACCTGC 1 cut(s) 471
BspPI GGATC 4 cut(s) 169, 182, 199, 237
BspT107I GGYRCC 1 cut(s) 277
BsrFI RCCGGY 2 cut(s) 5, 251
BssAI RCCGGY 2 cut(s) 5, 251
BssECI CCNNGG 3 cut(s) 159, 285, 381
BssMI GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
BssNI GRCGYC 3 cut(s) 92, 278, 305
BssT1I CCWWGG 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 160
Bst4CI ACNGT 4 cut(s) 14, 136, 289, 295
Bst6I CTCTTC 1 cut(s) 562
BstACI GRCGYC 3 cut(s) 92, 278, 305
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 27
BstDSI CCRYGG 1 cut(s) 285
BstF5I GGATG 1 cut(s) 551
BstFNI CGCG 1 cut(s) 502
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 2 cut(s) 280, 502
BstKTI GATC 7 cut(s) 60, 177, 194, 232, 259, 372, 428
BstMBI GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
BstMCI CGRYCG 2 cut(s) 345, 372
BstMWI GCNNNNNNNGC 2 cut(s) 458, 461
BstNI CCWGG 1 cut(s) 160
BstSCI CCNGG 1 cut(s) 158
BstUI CGCG 1 cut(s) 502
BstV1I GCAGC 5 cut(s) 55, 88, 439, 445, 448
BstV2I GAAGAC 1 cut(s) 153
BstX2I RGATCY 2 cut(s) 174, 425
BstYI RGATCY 2 cut(s) 174, 425
BsuRI GGCC 4 cut(s) 5, 248, 375, 440
BtgI CCRYGG 1 cut(s) 285
BtsCI GGATG 1 cut(s) 551
BveI ACCTGC 1 cut(s) 471
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 2 cut(s) 280, 502
Cfr10I RCCGGY 2 cut(s) 5, 251
Cfr13I GGNCC 2 cut(s) 365, 483
CseI GACGC 1 cut(s) 294
Csp6I GTAC 2 cut(s) 400, 567
CviAII CATG 2 cut(s) 64, 355
CviJI RGCY 9 cut(s) 5, 40, 68, 158, 248, 375, 440, 452, 458
CviKI_1 RGCY 9 cut(s) 5, 40, 68, 158, 248, 375, 440, 452, 458
CviQI GTAC 2 cut(s) 400, 567
DdeI CTNAG 1 cut(s) 27
DinI GGCGCC 1 cut(s) 279
DpnI GATC 7 cut(s) 59, 176, 193, 231, 258, 371, 427
DpnII GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
EaeI YGGCCR 2 cut(s) 3, 438
Eam1104I CTCTTC 1 cut(s) 562
EarI CTCTTC 1 cut(s) 562
Eco130I CCWWGG 1 cut(s) 381
Eco47I GGWCC 2 cut(s) 365, 483
Eco88I CYCGRG 1 cut(s) 153
EcoO109I RGGNCCY 1 cut(s) 483
EcoRII CCWGG 1 cut(s) 158
EcoT14I CCWWGG 1 cut(s) 381
EgeI GGCGCC 1 cut(s) 279
EheI GGCGCC 1 cut(s) 279
ErhI CCWWGG 1 cut(s) 381
FaeI CATG 2 cut(s) 67, 358
FaiI YATR 4 cut(s) 65, 227, 356, 522
FaqI GGGAC 1 cut(s) 518
FatI CATG 2 cut(s) 63, 354
FauI CCCGC 1 cut(s) 495
FokI GGATG 1 cut(s) 538
FspBI CTAG 1 cut(s) 564
GlaI GCGC 2 cut(s) 279, 501
GsaI CCCAGC 2 cut(s) 75, 191
HaeII RGCGCY 1 cut(s) 281
HaeIII GGCC 4 cut(s) 5, 248, 375, 440
HapII CCGG 4 cut(s) 6, 252, 345, 441
HgaI GACGC 1 cut(s) 294
HhaI GCGC 2 cut(s) 280, 502
Hin1I GRCGYC 3 cut(s) 92, 278, 305
Hin1II CATG 2 cut(s) 67, 358
Hin6I GCGC 2 cut(s) 278, 500
HinP1I GCGC 2 cut(s) 278, 500
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 1 cut(s) 149
HpaII CCGG 4 cut(s) 6, 252, 345, 441
HphI GGTGA 6 cut(s) 26, 46, 266, 301, 371, 554
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 3 cut(s) 57, 179, 369
Hpy188III TCNNGA 5 cut(s) 88, 153, 195, 240, 429
Hpy8I GTNNAC 1 cut(s) 142
Hpy99I CGWCG 4 cut(s) 94, 97, 340, 343
HpyAV CCTTC 2 cut(s) 291, 426
HpyCH4III ACNGT 4 cut(s) 14, 136, 289, 295
HpyCH4IV ACGT 2 cut(s) 92, 445
HpyCH4V TGCA 3 cut(s) 330, 394, 524
HpyF10VI GCNNNNNNNGC 2 cut(s) 458, 461
HpyF3I CTNAG 1 cut(s) 27
HpySE526I ACGT 2 cut(s) 92, 445
Hsp92I GRCGYC 3 cut(s) 92, 278, 305
Hsp92II CATG 2 cut(s) 67, 358
HspAI GCGC 2 cut(s) 278, 500
KasI GGCGCC 1 cut(s) 277
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
Kzo9I GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
Lsp1109I GCAGC 5 cut(s) 55, 88, 439, 445, 448
LweI GCATC 1 cut(s) 381
MaeI CTAG 1 cut(s) 564
MaeII ACGT 2 cut(s) 92, 445
MaeIII GTNAC 2 cut(s) 289, 419
MalI GATC 7 cut(s) 59, 176, 193, 231, 258, 371, 427
MboI GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
MboII GAAGA 4 cut(s) 119, 158, 400, 549
MflI RGATCY 2 cut(s) 174, 425
MluCI AATT 2 cut(s) 18, 82
Mly113I GGCGCC 1 cut(s) 278
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 1 cut(s) 448
MnlI CCTC 7 cut(s) 30, 112, 227, 390, 503, 532, 565
MroNI GCCGGC 1 cut(s) 5
MseI TTAA 1 cut(s) 574
MspI CCGG 4 cut(s) 6, 252, 345, 441
MspR9I CCNGG 1 cut(s) 160
MvaI CCWGG 1 cut(s) 160
MvnI CGCG 1 cut(s) 502
MwoI GCNNNNNNNGC 2 cut(s) 458, 461
NaeI GCCGGC 1 cut(s) 7
NarI GGCGCC 1 cut(s) 278
NdeII GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
NgoMIV GCCGGC 1 cut(s) 5
NlaIII CATG 2 cut(s) 67, 358
NlaIV GGNNCC 2 cut(s) 176, 279
NmuCI GTSAC 1 cut(s) 289
PaeR7I CTCGAG 1 cut(s) 153
PdiI GCCGGC 1 cut(s) 7
Ple19I CGATCG 1 cut(s) 372
PleI GAGTC 1 cut(s) 143
PluTI GGCGCC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 143
PpuMI RGGWCCY 1 cut(s) 483
Psp5II RGGWCCY 1 cut(s) 483
Psp6I CCWGG 1 cut(s) 158
PspFI CCCAGC 2 cut(s) 71, 187
PspGI CCWGG 1 cut(s) 158
PspN4I GGNNCC 2 cut(s) 176, 279
PspPI GGNCC 2 cut(s) 365, 483
PspPPI RGGWCCY 1 cut(s) 483
PsuI RGATCY 2 cut(s) 174, 425
PvuI CGATCG 1 cut(s) 372
RsaI GTAC 2 cut(s) 401, 568
RsaNI GTAC 2 cut(s) 400, 567
SaqAI TTAA 1 cut(s) 574
Sau3AI GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
Sau96I GGNCC 2 cut(s) 365, 483
ScaI AGTACT 1 cut(s) 568
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 1 cut(s) 160
SetI ASST 7 cut(s) 95, 123, 238, 401, 448, 485, 514
SfaNI GCATC 1 cut(s) 381
SfoI GGCGCC 1 cut(s) 279
Sfr274I CTCGAG 1 cut(s) 153
SgrAI CRCCGGYG 1 cut(s) 251
SinI GGWCC 2 cut(s) 365, 483
SlaI CTCGAG 1 cut(s) 153
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
Sse9I AATT 2 cut(s) 18, 82
SsiI CCGC 8 cut(s) 249, 275, 302, 415, 455, 494, 497, 502
SspDI GGCGCC 1 cut(s) 277
SspMI CTAG 1 cut(s) 564
StyD4I CCNGG 1 cut(s) 158
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 4 cut(s) 14, 136, 289, 295
TaiI ACGT 2 cut(s) 95, 448
TaqI TCGA 5 cut(s) 60, 123, 154, 232, 335
TasI AATT 2 cut(s) 18, 82
TatI WGTACW 1 cut(s) 566
TauI GCSGC 7 cut(s) 251, 278, 305, 418, 458, 497, 500
Tru1I TTAA 1 cut(s) 574
Tru9I TTAA 1 cut(s) 574
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 5 cut(s) 68, 76, 452, 458, 461
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 1 cut(s) 540
TspGWI ACGGA 2 cut(s) 397, 522
VpaK11BI GGWCC 2 cut(s) 365, 483
XapI RAATTY 2 cut(s) 18, 82
XcmI CCANNNNNNNNNTGG 1 cut(s) 292
XhoI CTCGAG 1 cut(s) 153
XspI CTAG 1 cut(s) 564
ZraI GACGTC 1 cut(s) 93
ZrmI AGTACT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.