Rw6G001690

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000535
Physical Location & Seq
Forward (+)
2654106 .. 2664971
10866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G001690.1

Sequence Viewer

Length: 447 bp
ATGGGTTCCAATCAGGAAGAGGTTTTTGGCCAGCTCGATGTGTACAAGTACGAGAAGGTAGTGCCGGAAGAGCAGTCCAAGAAGAACCGTCGATTTAACCAGGATGAAAATGTGTCATCGGATGATGATGATGACACCAAGGACGCTGGACTAAAAGGACTTTCCTTGATTCCTGATGGGTCGGGTGATGGAGGTTATGGGGATAACGCAATTGATTCGTGGAGGTGGAGGGGTTTTCTACCCTATGTTATTGGCATCAATGGCAGCCTAACAACCTACTTGAGCCCAGAGCATCATCGAGGGCCAGTCGCGGCTGCTGCTGCTGAAGTTGATGGAGGCAATCTAGTGTTGATAGACGCAGAAACGTTATGCACATGCATTGCCATAACCTTTCAGGTCCTCATCAAGTTTATGAACAGAGCATTTGCGTGGCCCCCTGGTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

16.18

Weight (kDa)

4.5

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 311
AciI CCGC 1 cut(s) 311
AclI AACGTT 1 cut(s) 365
AcoI YGGCCR 1 cut(s) 28
AcuI CTGAAG 1 cut(s) 345
AfaI GTAC 2 cut(s) 44, 50
AjnI CCWGG 2 cut(s) 99, 436
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AluBI AGCT 1 cut(s) 34
AluI AGCT 1 cut(s) 34
AoxI GGCC 3 cut(s) 28, 302, 431
ApeKI GCWGC 4 cut(s) 264, 314, 317, 320
AspS9I GGNCC 4 cut(s) 302, 397, 432, 440
AsuHPI GGTGA 1 cut(s) 197
AvaII GGWCC 2 cut(s) 397, 440
BalI TGGCCA 1 cut(s) 30
BanII GRGCYC 1 cut(s) 287
BbvI GCAGC 4 cut(s) 276, 301, 304, 307
BccI CCATC 3 cut(s) 170, 182, 326
BcgI CGANNNNNNTGC 2 cut(s) 198, 232
BciT130I CCWGG 2 cut(s) 101, 438
BfaI CTAG 1 cut(s) 344
BisI GCNGC 5 cut(s) 265, 312, 315, 318, 321
BlsI GCNGC 5 cut(s) 266, 313, 316, 319, 322
Bme1390I CCNGG 2 cut(s) 101, 438
Bme18I GGWCC 2 cut(s) 397, 440
BmgT120I GGNCC 4 cut(s) 302, 397, 432, 440
BmiI GGNNCC 2 cut(s) 7, 434
BmrFI CCNGG 2 cut(s) 101, 438
BmsI GCATC 2 cut(s) 264, 301
BpuEI CTTGAG 1 cut(s) 301
BsaJI CCNNGG 2 cut(s) 138, 436
Bse1I ACTGG 1 cut(s) 305
Bse3DI GCAATG 1 cut(s) 378
BseBI CCWGG 2 cut(s) 101, 438
BseDI CCNNGG 2 cut(s) 138, 436
BseGI GGATG 2 cut(s) 109, 127
BseMI GCAATG 1 cut(s) 378
BseNI ACTGG 1 cut(s) 305
BseXI GCAGC 4 cut(s) 276, 301, 304, 307
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 3 cut(s) 30, 304, 433
BsiSI CCGG 1 cut(s) 65
BsnI GGCC 3 cut(s) 30, 304, 433
Bsp1286I GDGCHC 1 cut(s) 287
Bsp1407I TGTACA 1 cut(s) 42
BspACI CCGC 1 cut(s) 311
BspANI GGCC 3 cut(s) 30, 304, 433
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 2 cut(s) 7, 434
BspQI GCTCTTC 1 cut(s) 63
BsrDI GCAATG 1 cut(s) 378
BsrGI TGTACA 1 cut(s) 42
BsrI ACTGG 1 cut(s) 305
BssECI CCNNGG 2 cut(s) 138, 436
BssT1I CCWWGG 1 cut(s) 138
Bst2UI CCWGG 2 cut(s) 101, 438
Bst4CI ACNGT 1 cut(s) 89
Bst6I CTCTTC 2 cut(s) 12, 63
BstAUI TGTACA 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 32
BstF5I GGATG 2 cut(s) 109, 127
BstFNI CGCG 1 cut(s) 311
BstMWI GCNNNNNNNGC 4 cut(s) 70, 261, 317, 320
BstNI CCWGG 2 cut(s) 101, 438
BstNSI RCATGY 1 cut(s) 378
BstSCI CCNGG 2 cut(s) 99, 436
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 4 cut(s) 276, 301, 304, 307
BsuRI GGCC 3 cut(s) 30, 304, 433
BtsCI GGATG 2 cut(s) 109, 127
Cac8I GCNNGC 1 cut(s) 32
Cfr13I GGNCC 4 cut(s) 302, 397, 432, 440
CseI GACGC 2 cut(s) 152, 365
Csp6I GTAC 2 cut(s) 43, 49
CviAII CATG 1 cut(s) 375
CviJI RGCY 7 cut(s) 30, 34, 267, 285, 304, 314, 433
CviKI_1 RGCY 7 cut(s) 30, 34, 267, 285, 304, 314, 433
CviQI GTAC 2 cut(s) 43, 49
EaeI YGGCCR 1 cut(s) 28
Eam1104I CTCTTC 2 cut(s) 12, 63
EarI CTCTTC 2 cut(s) 12, 63
Eco130I CCWWGG 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 287
Eco47I GGWCC 2 cut(s) 397, 440
Eco57I CTGAAG 1 cut(s) 345
EcoO109I RGGNCCY 1 cut(s) 397
EcoRII CCWGG 2 cut(s) 99, 436
EcoT14I CCWWGG 1 cut(s) 138
EcoT22I ATGCAT 1 cut(s) 380
EcoT38I GRGCYC 1 cut(s) 287
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 1 cut(s) 378
FaiI YATR 6 cut(s) 198, 246, 370, 376, 386, 413
FatI CATG 1 cut(s) 374
Fnu4HI GCNGC 5 cut(s) 265, 312, 315, 318, 321
FokI GGATG 2 cut(s) 116, 134
FriOI GRGCYC 1 cut(s) 287
Fsp4HI GCNGC 5 cut(s) 265, 312, 315, 318, 321
FspBI CTAG 1 cut(s) 344
GluI GCNGC 5 cut(s) 265, 312, 315, 318, 321
HaeIII GGCC 3 cut(s) 30, 304, 433
HapII CCGG 1 cut(s) 65
HgaI GACGC 2 cut(s) 152, 365
Hin1II CATG 1 cut(s) 378
HinfI GANTC 2 cut(s) 169, 215
HpaII CCGG 1 cut(s) 65
HphI GGTGA 1 cut(s) 197
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 2 cut(s) 14, 173
Hpy8I GTNNAC 1 cut(s) 43
Hpy99I CGWCG 1 cut(s) 93
HpyAV CCTTC 1 cut(s) 49
HpyCH4III ACNGT 1 cut(s) 89
HpyCH4IV ACGT 1 cut(s) 365
HpyCH4V TGCA 2 cut(s) 372, 378
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 261, 317, 320
HpySE526I ACGT 1 cut(s) 365
Hsp92II CATG 1 cut(s) 378
LguI GCTCTTC 1 cut(s) 63
Lsp1109I GCAGC 4 cut(s) 276, 301, 304, 307
LweI GCATC 2 cut(s) 264, 301
MaeI CTAG 1 cut(s) 344
MaeII ACGT 1 cut(s) 365
MboII GAAGA 3 cut(s) 29, 80, 94
MfeI CAATTG 1 cut(s) 210
MhlI GDGCHC 1 cut(s) 287
MlsI TGGCCA 1 cut(s) 30
MluCI AATT 1 cut(s) 210
MluNI TGGCCA 1 cut(s) 30
MnlI CCTC 7 cut(s) 13, 185, 216, 222, 293, 329, 410
Mox20I TGGCCA 1 cut(s) 30
Mph1103I ATGCAT 1 cut(s) 380
MscI TGGCCA 1 cut(s) 30
MseI TTAA 2 cut(s) 96, 445
MslI CAYNNNNRTG 1 cut(s) 427
Msp20I TGGCCA 1 cut(s) 30
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 2 cut(s) 101, 438
MunI CAATTG 1 cut(s) 210
MvaI CCWGG 2 cut(s) 101, 438
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 4 cut(s) 70, 261, 317, 320
NlaIII CATG 1 cut(s) 378
NlaIV GGNNCC 2 cut(s) 7, 434
NsiI ATGCAT 1 cut(s) 380
NspI RCATGY 1 cut(s) 378
PciSI GCTCTTC 1 cut(s) 63
PfeI GAWTC 2 cut(s) 169, 215
PkrI GCNGC 5 cut(s) 266, 313, 316, 319, 322
PpuMI RGGWCCY 1 cut(s) 397
Psp1406I AACGTT 1 cut(s) 365
Psp5II RGGWCCY 1 cut(s) 397
Psp6I CCWGG 2 cut(s) 99, 436
PspGI CCWGG 2 cut(s) 99, 436
PspN4I GGNNCC 2 cut(s) 7, 434
PspPI GGNCC 4 cut(s) 302, 397, 432, 440
PspPPI RGGWCCY 1 cut(s) 397
RsaI GTAC 2 cut(s) 44, 50
RsaNI GTAC 2 cut(s) 43, 49
RseI CAYNNNNRTG 1 cut(s) 427
SapI GCTCTTC 1 cut(s) 63
SaqAI TTAA 2 cut(s) 96, 445
SatI GCNGC 5 cut(s) 265, 312, 315, 318, 321
Sau96I GGNCC 4 cut(s) 302, 397, 432, 440
ScrFI CCNGG 2 cut(s) 101, 438
SduI GDGCHC 1 cut(s) 287
SetI ASST 9 cut(s) 24, 36, 60, 196, 227, 278, 368, 392, 399
SfaNI GCATC 2 cut(s) 264, 301
SinI GGWCC 2 cut(s) 397, 440
SmiMI CAYNNNNRTG 1 cut(s) 427
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
Sse9I AATT 1 cut(s) 210
SsiI CCGC 1 cut(s) 311
SspMI CTAG 1 cut(s) 344
StyD4I CCNGG 2 cut(s) 99, 436
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 1 cut(s) 89
TaiI ACGT 1 cut(s) 368
TaqI TCGA 3 cut(s) 36, 91, 298
TasI AATT 1 cut(s) 210
TatI WGTACW 1 cut(s) 42
TauI GCSGC 1 cut(s) 314
TfiI GAWTC 2 cut(s) 169, 215
Tru1I TTAA 2 cut(s) 96, 445
Tru9I TTAA 2 cut(s) 96, 445
TseI GCWGC 4 cut(s) 264, 314, 317, 320
TspDTI ATGAA 2 cut(s) 120, 428
VpaK11BI GGWCC 2 cut(s) 397, 440
XceI RCATGY 1 cut(s) 378
XspI CTAG 1 cut(s) 344
Zsp2I ATGCAT 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.