Rh7DG359100

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000535
Physical Location & Seq
Forward (+)
47813734 .. 47819485
5752 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG359100.1

Sequence Viewer

Length: 777 bp
ATGGCCGGCAACGGTGAAATTCTGACTAAGGGGGTGAGGCTCTCTCCTTTCTCTCTGATTGACATGGCTGCTGGGAAGCGAAATTTCCCGACGTCGGTTGGGATTGAAGATGGTTTGGAGGTCGATTTTGCTACTGTGGTTGACGAAGACTCTCGAGCCTGGGTTGTTGCTGGGATCCGATTTCTGCTGGGATCAGGATTGGGTCGGCAATCTTTGTTTGTCTTTATGGATCGAGGTTCTGGAAAGGCCGCCGGTGATCTGGTGTTTTTGGTGGCGGCGCCTTCTACGGTGACGGTGGTAGCGGCGTCTATTGTGGTGGGTCAATGGTGCATTTCGACGACGACCGGAAAGGGCATGGGTGATGATCCGATCGGCCTTGCCTTGGCGAAGATGCAGAGGTACTTTTCCGTTGGGCGGCGATACAGATCTGGAAGGGTGGCCGGAAGTTGGGCTGCGGCTGCTGCTGTTGGAGTTGATGGCAGGTCCTTTACGGCGGCGGCGCGGGACGGAGGTGGTGGTTATGCAGAATCAACCTATCAGTATGCTTCTACCAGAGGATTCAGTATTAGAAGAGATTCAGATGATGTTAGTTATTCTGGAATAAAGGATTGGAACTGTCCATGGGGTTCCACTGTGGATGATGATGTAGCTCCAGAGTTTAGATTGATTTGGAGGGGCACTTCGGCATCTGTGAAACATCCTGTACAAGATACAGATGAGAAAAAGCTTCACTGGTGGGTGCAGAGGAAGAATTTTGATCGCCATGCACCTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

258

Amino Acids

27.81

Weight (kDa)

8.53

Isoelectric Point (pI)

28.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 95
Acc36I ACCTGC 1 cut(s) 471
AccB1I GGYRCC 1 cut(s) 277
AccII CGCG 1 cut(s) 502
AciI CCGC 8 cut(s) 249, 275, 302, 415, 455, 494, 497, 502
AclWI GGATC 5 cut(s) 169, 182, 199, 237, 359
AcoI YGGCCR 2 cut(s) 3, 438
AcsI RAATTY 3 cut(s) 18, 82, 751
AcyI GRCGYC 3 cut(s) 92, 278, 305
AfaI GTAC 2 cut(s) 401, 705
AfiI CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
AgsI TTSAA 1 cut(s) 107
AjnI CCWGG 1 cut(s) 158
AjuI GAANNNNNNNTTGG 2 cut(s) 592, 624
AluBI AGCT 2 cut(s) 650, 727
AluI AGCT 2 cut(s) 650, 727
AlwI GGATC 5 cut(s) 169, 182, 199, 237, 359
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 4 cut(s) 3, 246, 373, 438
ApeKI GCWGC 4 cut(s) 68, 452, 458, 461
ApoI RAATTY 3 cut(s) 18, 82, 751
Asp700I GAANNNNTTC 1 cut(s) 574
AspLEI GCGC 2 cut(s) 280, 502
AspS9I GGNCC 1 cut(s) 483
AsuHPI GGTGA 5 cut(s) 26, 46, 266, 301, 371
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 1 cut(s) 483
BaeGI GKGCMC 1 cut(s) 680
BamHI GGATCC 1 cut(s) 174
BanI GGYRCC 1 cut(s) 277
BbsI GAAGAC 1 cut(s) 153
BbvI GCAGC 4 cut(s) 55, 439, 445, 448
BccI CCATC 2 cut(s) 104, 470
BceAI ACGGC 1 cut(s) 507
BciT130I CCWGG 1 cut(s) 160
BfoI RGCGCY 1 cut(s) 281
BfuAI ACCTGC 1 cut(s) 471
BglII AGATCT 1 cut(s) 425
Bme1390I CCNGG 1 cut(s) 160
Bme18I GGWCC 1 cut(s) 483
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 1 cut(s) 483
BmiI GGNNCC 3 cut(s) 176, 279, 628
BmrFI CCNGG 1 cut(s) 160
BmsI GCATC 2 cut(s) 381, 695
BpiI GAAGAC 1 cut(s) 153
BplI GAGNNNNNCTC 2 cut(s) 28, 60
BpmI CTGGAG 1 cut(s) 636
BsaBI GATNNNNATC 1 cut(s) 424
BsaHI GRCGYC 3 cut(s) 92, 278, 305
BsaJI CCNNGG 4 cut(s) 159, 381, 620, 770
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
Bse118I RCCGGY 2 cut(s) 5, 251
Bse1I ACTGG 1 cut(s) 737
Bse8I GATNNNNATC 1 cut(s) 424
BseBI CCWGG 1 cut(s) 160
BseDI CCNNGG 4 cut(s) 159, 381, 620, 770
BseGI GGATG 2 cut(s) 643, 697
BseJI GATNNNNATC 1 cut(s) 424
BseLI CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
BseNI ACTGG 1 cut(s) 737
BseSI GKGCMC 1 cut(s) 680
BseXI GCAGC 4 cut(s) 55, 439, 445, 448
BseYI CCCAGC 3 cut(s) 71, 170, 187
BsgI GTGCAG 1 cut(s) 761
Bsh1236I CGCG 1 cut(s) 502
Bsh1285I CGRYCG 2 cut(s) 345, 372
BshFI GGCC 4 cut(s) 5, 248, 375, 440
BshNI GGYRCC 1 cut(s) 277
BsiEI CGRYCG 2 cut(s) 345, 372
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 4 cut(s) 6, 252, 345, 441
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
BsmFI GGGAC 1 cut(s) 518
BsnI GGCC 4 cut(s) 5, 248, 375, 440
BsoBI CYCGRG 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 680
Bsp1407I TGTACA 1 cut(s) 703
Bsp143I GATC 8 cut(s) 174, 191, 229, 256, 364, 369, 425, 757
Bsp19I CCATGG 1 cut(s) 620
BspACI CCGC 8 cut(s) 249, 275, 302, 415, 455, 494, 497, 502
BspANI GGCC 4 cut(s) 5, 248, 375, 440
BspFNI CGCG 1 cut(s) 502
BspLI GGNNCC 3 cut(s) 176, 279, 628
BspMI ACCTGC 1 cut(s) 471
BspPI GGATC 5 cut(s) 169, 182, 199, 237, 359
BspT107I GGYRCC 1 cut(s) 277
BsrFI RCCGGY 2 cut(s) 5, 251
BsrGI TGTACA 1 cut(s) 703
BsrI ACTGG 1 cut(s) 737
BssAI RCCGGY 2 cut(s) 5, 251
BssECI CCNNGG 4 cut(s) 159, 381, 620, 770
BssMI GATC 8 cut(s) 174, 191, 229, 256, 364, 369, 425, 757
BssNI GRCGYC 3 cut(s) 92, 278, 305
BssT1I CCWWGG 3 cut(s) 381, 620, 770
Bst2UI CCWGG 1 cut(s) 160
Bst4CI ACNGT 6 cut(s) 14, 136, 289, 295, 617, 634
Bst6I CTCTTC 1 cut(s) 565
BstACI GRCGYC 3 cut(s) 92, 278, 305
BstAUI TGTACA 1 cut(s) 703
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 27
BstDSI CCRYGG 1 cut(s) 620
BstF5I GGATG 2 cut(s) 643, 697
BstFNI CGCG 1 cut(s) 502
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 2 cut(s) 280, 502
BstKTI GATC 8 cut(s) 177, 194, 232, 259, 367, 372, 428, 760
BstMBI GATC 8 cut(s) 174, 191, 229, 256, 364, 369, 425, 757
BstMCI CGRYCG 2 cut(s) 345, 372
BstMWI GCNNNNNNNGC 2 cut(s) 458, 461
BstNI CCWGG 1 cut(s) 160
BstSCI CCNGG 1 cut(s) 158
BstSLI GKGCMC 1 cut(s) 680
BstUI CGCG 1 cut(s) 502
BstV1I GCAGC 4 cut(s) 55, 439, 445, 448
BstV2I GAAGAC 1 cut(s) 153
BstX2I RGATCY 2 cut(s) 174, 425
BstYI RGATCY 2 cut(s) 174, 425
BsuRI GGCC 4 cut(s) 5, 248, 375, 440
BtgI CCRYGG 1 cut(s) 620
BtsCI GGATG 2 cut(s) 643, 697
BtsIMutI CAGTG 2 cut(s) 630, 730
BveI ACCTGC 1 cut(s) 471
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 2 cut(s) 280, 502
Cfr10I RCCGGY 2 cut(s) 5, 251
Cfr13I GGNCC 1 cut(s) 483
CseI GACGC 1 cut(s) 294
Csp6I GTAC 2 cut(s) 400, 704
CviAII CATG 4 cut(s) 64, 355, 621, 764
CviQI GTAC 2 cut(s) 400, 704
DdeI CTNAG 1 cut(s) 27
DinI GGCGCC 1 cut(s) 279
DpnI GATC 8 cut(s) 176, 193, 231, 258, 366, 371, 427, 759
DpnII GATC 8 cut(s) 174, 191, 229, 256, 364, 369, 425, 757
EaeI YGGCCR 2 cut(s) 3, 438
Eam1104I CTCTTC 1 cut(s) 565
EarI CTCTTC 1 cut(s) 565
Eco130I CCWWGG 3 cut(s) 381, 620, 770
Eco47I GGWCC 1 cut(s) 483
Eco88I CYCGRG 1 cut(s) 153
EcoO109I RGGNCCY 1 cut(s) 483
EcoRII CCWGG 1 cut(s) 158
EcoT14I CCWWGG 3 cut(s) 381, 620, 770
EgeI GGCGCC 1 cut(s) 279
EheI GGCGCC 1 cut(s) 279
ErhI CCWWGG 3 cut(s) 381, 620, 770
FaeI CATG 4 cut(s) 67, 358, 624, 767
FaiI YATR 7 cut(s) 65, 227, 356, 522, 543, 622, 765
FaqI GGGAC 1 cut(s) 518
FatI CATG 4 cut(s) 63, 354, 620, 763
FauI CCCGC 1 cut(s) 495
FokI GGATG 2 cut(s) 650, 684
GlaI GCGC 2 cut(s) 279, 501
GsaI CCCAGC 3 cut(s) 75, 174, 191
GsuI CTGGAG 1 cut(s) 636
HaeII RGCGCY 1 cut(s) 281
HaeIII GGCC 4 cut(s) 5, 248, 375, 440
HapII CCGG 4 cut(s) 6, 252, 345, 441
HgaI GACGC 1 cut(s) 294
HhaI GCGC 2 cut(s) 280, 502
Hin1I GRCGYC 3 cut(s) 92, 278, 305
Hin1II CATG 4 cut(s) 67, 358, 624, 767
Hin6I GCGC 2 cut(s) 278, 500
HinP1I GCGC 2 cut(s) 278, 500
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 725
HinfI GANTC 4 cut(s) 149, 527, 558, 575
HpaII CCGG 4 cut(s) 6, 252, 345, 441
HphI GGTGA 5 cut(s) 26, 46, 266, 301, 371
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 5 cut(s) 24, 57, 179, 369, 580
Hpy188III TCNNGA 7 cut(s) 88, 153, 195, 240, 429, 597, 653
Hpy8I GTNNAC 1 cut(s) 142
Hpy99I CGWCG 4 cut(s) 94, 97, 340, 343
HpyAV CCTTC 2 cut(s) 291, 426
HpyCH4III ACNGT 6 cut(s) 14, 136, 289, 295, 617, 634
HpyCH4IV ACGT 1 cut(s) 92
HpyCH4V TGCA 5 cut(s) 330, 394, 524, 742, 767
HpyF10VI GCNNNNNNNGC 2 cut(s) 458, 461
HpyF3I CTNAG 1 cut(s) 27
HpySE526I ACGT 1 cut(s) 92
Hsp92I GRCGYC 3 cut(s) 92, 278, 305
Hsp92II CATG 4 cut(s) 67, 358, 624, 767
HspAI GCGC 2 cut(s) 278, 500
KasI GGCGCC 1 cut(s) 277
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
Kzo9I GATC 8 cut(s) 174, 191, 229, 256, 364, 369, 425, 757
LmnI GCTCC 1 cut(s) 655
Lsp1109I GCAGC 4 cut(s) 55, 439, 445, 448
LweI GCATC 2 cut(s) 381, 695
MaeII ACGT 1 cut(s) 92
MaeIII GTNAC 1 cut(s) 289
MalI GATC 8 cut(s) 176, 193, 231, 258, 366, 371, 427, 759
MboI GATC 8 cut(s) 174, 191, 229, 256, 364, 369, 425, 757
MboII GAAGA 5 cut(s) 119, 158, 400, 582, 760
MflI RGATCY 2 cut(s) 174, 425
MhlI GDGCHC 1 cut(s) 680
MluCI AATT 3 cut(s) 18, 82, 751
Mly113I GGCGCC 1 cut(s) 278
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 1 cut(s) 448
MnlI CCTC 8 cut(s) 30, 112, 227, 390, 503, 548, 666, 738
MroNI GCCGGC 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 574
MspI CCGG 4 cut(s) 6, 252, 345, 441
MspR9I CCNGG 1 cut(s) 160
MvaI CCWGG 1 cut(s) 160
MvnI CGCG 1 cut(s) 502
MwoI GCNNNNNNNGC 2 cut(s) 458, 461
NaeI GCCGGC 1 cut(s) 7
NarI GGCGCC 1 cut(s) 278
NcoI CCATGG 1 cut(s) 620
NdeII GATC 8 cut(s) 174, 191, 229, 256, 364, 369, 425, 757
NgoMIV GCCGGC 1 cut(s) 5
NlaIII CATG 4 cut(s) 67, 358, 624, 767
NlaIV GGNNCC 3 cut(s) 176, 279, 628
NmuCI GTSAC 1 cut(s) 289
PaeR7I CTCGAG 1 cut(s) 153
PdiI GCCGGC 1 cut(s) 7
PdmI GAANNNNTTC 1 cut(s) 574
PfeI GAWTC 3 cut(s) 527, 558, 575
Ple19I CGATCG 1 cut(s) 372
PleI GAGTC 1 cut(s) 143
PluTI GGCGCC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 143
PpuMI RGGWCCY 1 cut(s) 483
Psp5II RGGWCCY 1 cut(s) 483
Psp6I CCWGG 1 cut(s) 158
PspFI CCCAGC 3 cut(s) 71, 170, 187
PspGI CCWGG 1 cut(s) 158
PspN4I GGNNCC 3 cut(s) 176, 279, 628
PspPI GGNCC 1 cut(s) 483
PspPPI RGGWCCY 1 cut(s) 483
PsuI RGATCY 2 cut(s) 174, 425
PvuI CGATCG 1 cut(s) 372
RsaI GTAC 2 cut(s) 401, 705
RsaNI GTAC 2 cut(s) 400, 704
Sau3AI GATC 8 cut(s) 174, 191, 229, 256, 364, 369, 425, 757
Sau96I GGNCC 1 cut(s) 483
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 1 cut(s) 160
SduI GDGCHC 1 cut(s) 680
SfaNI GCATC 2 cut(s) 381, 695
SfoI GGCGCC 1 cut(s) 279
Sfr274I CTCGAG 1 cut(s) 153
SgrAI CRCCGGYG 1 cut(s) 251
SinI GGWCC 1 cut(s) 483
SlaI CTCGAG 1 cut(s) 153
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
Sse9I AATT 3 cut(s) 18, 82, 751
SsiI CCGC 8 cut(s) 249, 275, 302, 415, 455, 494, 497, 502
SspDI GGCGCC 1 cut(s) 277
StyD4I CCNGG 1 cut(s) 158
StyI CCWWGG 3 cut(s) 381, 620, 770
TaaI ACNGT 6 cut(s) 14, 136, 289, 295, 617, 634
TaiI ACGT 1 cut(s) 95
TaqI TCGA 4 cut(s) 123, 154, 232, 335
TasI AATT 3 cut(s) 18, 82, 751
TatI WGTACW 1 cut(s) 703
TauI GCSGC 7 cut(s) 251, 278, 305, 418, 458, 497, 500
TfiI GAWTC 3 cut(s) 527, 558, 575
TscAI CASTG 2 cut(s) 637, 737
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 4 cut(s) 68, 452, 458, 461
Tsp45I GTSAC 1 cut(s) 289
TspGWI ACGGA 2 cut(s) 397, 522
TspRI CASTG 2 cut(s) 637, 737
VpaK11BI GGWCC 1 cut(s) 483
XapI RAATTY 3 cut(s) 18, 82, 751
XhoI CTCGAG 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 574
ZraI GACGTC 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.