Rw0G016220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00751
OG0000535
Physical Location & Seq
Forward (+)
165547 .. 167416
1870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G016220.1

Sequence Viewer

Length: 498 bp
ATGGCCGGCAACGGTGAAATTCTGACTAAGGGGGTGAGGCTCTCTCCTTTCTCTCTGATCGACATGGCTGCTGGGCAGCGAAATTTCCCAACGTCGGTTGGGATTGAAGATGGTTTGGAGGTCGATTTTGCTACTGTGGTTGACGAAGACTCTCGAGCCTGGGTTGTTGCTGGGATCCGATTTCTGCTGGGATCAGGATTGGGTCGGCAATCTTTGTTTGTCGTTATGGATCGAGGTTCTGGAAAGGCCGCCGGTGATCTGGTGTTTTTGGTGGCGGCGCTTTCCACGGTGACGGTGGTAGCGGCGTCTATTGTGGTGGGTCAATGGTGCATTTCGACGACGACCGGAAAGGGCATGGGTGATGGTCCGATCGGCCTTGCCTTGGCGAAGATGCAGAGGTACTTTTCCGTTGGGCGGCGTTACAGATCTGGAAGGGTGGCCGGAAGTTGGGCTGCAGCTGCTGCTGTTGGAGTTGATGGCAGAGAAGGAACACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

17.07

Weight (kDa)

8.97

Isoelectric Point (pI)

20.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 249, 275, 302, 415
AclWI GGATC 4 cut(s) 169, 182, 199, 237
AcoI YGGCCR 2 cut(s) 3, 438
AcsI RAATTY 2 cut(s) 18, 82
AcyI GRCGYC 1 cut(s) 305
AfaI GTAC 1 cut(s) 401
AfiI CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
AgsI TTSAA 1 cut(s) 107
AjnI CCWGG 1 cut(s) 158
AluBI AGCT 1 cut(s) 458
AluI AGCT 1 cut(s) 458
AlwI GGATC 4 cut(s) 169, 182, 199, 237
AlwNI CAGNNNCTG 1 cut(s) 461
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 4 cut(s) 3, 246, 373, 438
ApeKI GCWGC 6 cut(s) 68, 76, 452, 455, 458, 461
ApoI RAATTY 2 cut(s) 18, 82
AspLEI GCGC 1 cut(s) 280
AspS9I GGNCC 1 cut(s) 365
AsuHPI GGTGA 5 cut(s) 26, 46, 266, 301, 371
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 1 cut(s) 365
BamHI GGATCC 1 cut(s) 174
BbsI GAAGAC 1 cut(s) 153
BbvI GCAGC 6 cut(s) 55, 88, 439, 445, 448, 467
BccI CCATC 3 cut(s) 104, 356, 470
BcgI CGANNNNNNTGC 2 cut(s) 50, 84
BciT130I CCWGG 1 cut(s) 160
BfaI CTAG 1 cut(s) 496
BfmI CTRYAG 1 cut(s) 453
BfoI RGCGCY 1 cut(s) 281
BglII AGATCT 1 cut(s) 425
Bme1390I CCNGG 1 cut(s) 160
Bme18I GGWCC 1 cut(s) 365
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 1 cut(s) 365
BmiI GGNNCC 1 cut(s) 176
BmrFI CCNGG 1 cut(s) 160
BmsI GCATC 1 cut(s) 381
BpiI GAAGAC 1 cut(s) 153
BplI GAGNNNNNCTC 2 cut(s) 28, 60
BsaHI GRCGYC 1 cut(s) 305
BsaJI CCNNGG 3 cut(s) 159, 285, 381
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
Bse118I RCCGGY 2 cut(s) 5, 251
BseBI CCWGG 1 cut(s) 160
BseDI CCNNGG 3 cut(s) 159, 285, 381
BseLI CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
BseXI GCAGC 6 cut(s) 55, 88, 439, 445, 448, 467
BseYI CCCAGC 3 cut(s) 71, 170, 187
Bsh1285I CGRYCG 2 cut(s) 345, 372
BshFI GGCC 4 cut(s) 5, 248, 375, 440
BsiEI CGRYCG 2 cut(s) 345, 372
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 4 cut(s) 6, 252, 345, 441
BslI CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
BsnI GGCC 4 cut(s) 5, 248, 375, 440
BsoBI CYCGRG 1 cut(s) 153
Bsp143I GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
BspACI CCGC 4 cut(s) 249, 275, 302, 415
BspANI GGCC 4 cut(s) 5, 248, 375, 440
BspLI GGNNCC 1 cut(s) 176
BspMAI CTGCAG 1 cut(s) 457
BspPI GGATC 4 cut(s) 169, 182, 199, 237
BsrFI RCCGGY 2 cut(s) 5, 251
BssAI RCCGGY 2 cut(s) 5, 251
BssECI CCNNGG 3 cut(s) 159, 285, 381
BssMI GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
BssNI GRCGYC 1 cut(s) 305
BssT1I CCWWGG 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 160
Bst4CI ACNGT 4 cut(s) 14, 136, 289, 295
BstACI GRCGYC 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 461
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 27
BstDSI CCRYGG 1 cut(s) 285
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 1 cut(s) 280
BstKTI GATC 7 cut(s) 60, 177, 194, 232, 259, 372, 428
BstMBI GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
BstMCI CGRYCG 2 cut(s) 345, 372
BstMWI GCNNNNNNNGC 2 cut(s) 458, 461
BstNI CCWGG 1 cut(s) 160
BstSCI CCNGG 1 cut(s) 158
BstSFI CTRYAG 1 cut(s) 453
BstV1I GCAGC 6 cut(s) 55, 88, 439, 445, 448, 467
BstV2I GAAGAC 1 cut(s) 153
BstX2I RGATCY 2 cut(s) 174, 425
BstYI RGATCY 2 cut(s) 174, 425
BsuRI GGCC 4 cut(s) 5, 248, 375, 440
BtgI CCRYGG 1 cut(s) 285
Cac8I GCNNGC 1 cut(s) 7
CaiI CAGNNNCTG 1 cut(s) 461
CfoI GCGC 1 cut(s) 280
Cfr10I RCCGGY 2 cut(s) 5, 251
Cfr13I GGNCC 1 cut(s) 365
CseI GACGC 1 cut(s) 294
Csp6I GTAC 1 cut(s) 400
CviAII CATG 2 cut(s) 64, 355
CviJI RGCY 9 cut(s) 5, 40, 68, 158, 248, 375, 440, 452, 458
CviKI_1 RGCY 9 cut(s) 5, 40, 68, 158, 248, 375, 440, 452, 458
CviQI GTAC 1 cut(s) 400
DdeI CTNAG 1 cut(s) 27
DpnI GATC 7 cut(s) 59, 176, 193, 231, 258, 371, 427
DpnII GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
EaeI YGGCCR 2 cut(s) 3, 438
Eco130I CCWWGG 1 cut(s) 381
Eco47I GGWCC 1 cut(s) 365
Eco88I CYCGRG 1 cut(s) 153
EcoRII CCWGG 1 cut(s) 158
EcoT14I CCWWGG 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 381
FaeI CATG 2 cut(s) 67, 358
FaiI YATR 3 cut(s) 65, 227, 356
FatI CATG 2 cut(s) 63, 354
FspBI CTAG 1 cut(s) 496
GlaI GCGC 1 cut(s) 279
GsaI CCCAGC 3 cut(s) 75, 174, 191
HaeII RGCGCY 1 cut(s) 281
HaeIII GGCC 4 cut(s) 5, 248, 375, 440
HapII CCGG 4 cut(s) 6, 252, 345, 441
HgaI GACGC 1 cut(s) 294
HhaI GCGC 1 cut(s) 280
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 2 cut(s) 67, 358
Hin6I GCGC 1 cut(s) 278
HinP1I GCGC 1 cut(s) 278
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 1 cut(s) 149
HpaII CCGG 4 cut(s) 6, 252, 345, 441
HphI GGTGA 5 cut(s) 26, 46, 266, 301, 371
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 4 cut(s) 24, 57, 179, 369
Hpy188III TCNNGA 4 cut(s) 153, 195, 240, 429
Hpy8I GTNNAC 1 cut(s) 142
Hpy99I CGWCG 3 cut(s) 97, 340, 343
HpyAV CCTTC 2 cut(s) 426, 479
HpyCH4III ACNGT 4 cut(s) 14, 136, 289, 295
HpyCH4IV ACGT 1 cut(s) 92
HpyCH4V TGCA 3 cut(s) 330, 394, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 458, 461
HpyF3I CTNAG 1 cut(s) 27
HpySE526I ACGT 1 cut(s) 92
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 2 cut(s) 67, 358
HspAI GCGC 1 cut(s) 278
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
Kzo9I GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
Lsp1109I GCAGC 6 cut(s) 55, 88, 439, 445, 448, 467
LweI GCATC 1 cut(s) 381
MaeI CTAG 1 cut(s) 496
MaeII ACGT 1 cut(s) 92
MaeIII GTNAC 2 cut(s) 289, 419
MalI GATC 7 cut(s) 59, 176, 193, 231, 258, 371, 427
MboI GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
MboII GAAGA 3 cut(s) 119, 158, 400
MflI RGATCY 2 cut(s) 174, 425
MluCI AATT 2 cut(s) 18, 82
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 1 cut(s) 448
MnlI CCTC 4 cut(s) 30, 112, 227, 390
MroNI GCCGGC 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 458
MspI CCGG 4 cut(s) 6, 252, 345, 441
MspR9I CCNGG 1 cut(s) 160
MvaI CCWGG 1 cut(s) 160
MwoI GCNNNNNNNGC 2 cut(s) 458, 461
NaeI GCCGGC 1 cut(s) 7
NdeII GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
NgoMIV GCCGGC 1 cut(s) 5
NlaIII CATG 2 cut(s) 67, 358
NlaIV GGNNCC 1 cut(s) 176
NmuCI GTSAC 1 cut(s) 289
PaeR7I CTCGAG 1 cut(s) 153
PdiI GCCGGC 1 cut(s) 7
Ple19I CGATCG 1 cut(s) 372
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 158
PspFI CCCAGC 3 cut(s) 71, 170, 187
PspGI CCWGG 1 cut(s) 158
PspN4I GGNNCC 1 cut(s) 176
PspPI GGNCC 1 cut(s) 365
PstI CTGCAG 1 cut(s) 457
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 2 cut(s) 174, 425
PvuI CGATCG 1 cut(s) 372
PvuII CAGCTG 1 cut(s) 458
RsaI GTAC 1 cut(s) 401
RsaNI GTAC 1 cut(s) 400
Sau3AI GATC 7 cut(s) 57, 174, 191, 229, 256, 369, 425
Sau96I GGNCC 1 cut(s) 365
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 1 cut(s) 160
SetI ASST 5 cut(s) 95, 123, 238, 401, 460
SfaNI GCATC 1 cut(s) 381
SfcI CTRYAG 1 cut(s) 453
Sfr274I CTCGAG 1 cut(s) 153
SgrAI CRCCGGYG 1 cut(s) 251
SinI GGWCC 1 cut(s) 365
SlaI CTCGAG 1 cut(s) 153
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
Sse9I AATT 2 cut(s) 18, 82
SsiI CCGC 4 cut(s) 249, 275, 302, 415
SspMI CTAG 1 cut(s) 496
StyD4I CCNGG 1 cut(s) 158
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 4 cut(s) 14, 136, 289, 295
TaiI ACGT 1 cut(s) 95
TaqI TCGA 5 cut(s) 60, 123, 154, 232, 335
TasI AATT 2 cut(s) 18, 82
TauI GCSGC 4 cut(s) 251, 278, 305, 418
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 6 cut(s) 68, 76, 452, 455, 458, 461
Tsp45I GTSAC 1 cut(s) 289
TspGWI ACGGA 1 cut(s) 397
VpaK11BI GGWCC 1 cut(s) 365
XapI RAATTY 2 cut(s) 18, 82
XcmI CCANNNNNNNNNTGG 1 cut(s) 292
XhoI CTCGAG 1 cut(s) 153
XspI CTAG 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.