Rw0G006280

Component of the coenzyme Q biosynthetic pathway. May play a role in organizing a multi-subunit COQ enzyme complex required for coenzyme Q biosynthesis. Required for steady-state levels of other COQ polypeptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00245
OG0000535
Physical Location & Seq
Forward (+)
34993 .. 39432
4440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G006280.1

Sequence Viewer

Length: 1236 bp
ATGGCCGGCAACGGTGAAATTCTGACTAAGGGGGTGAGGCTCTCTCCTTTCTCTCTGATCGACATGGCTGCTGGGCAGCGAAATTTCCCAACGTCGGTTGGGATTGAAGATGGTTTGGAGGTCGATTTTGCTACTGTGGTTGACGAAGACTCTCGAGCCTGGGTTGTTGCTGGGATCCGATTTCTGCTGGGATCAGGATTGGGTCGGCAATCTTTGTTTGTCGTTATGGATCGAGGTTCTGGAAAGGCCGCCGGTGATCTGGTGTTTTTGGTGGCGGCGCTTTCCACGGTGACGGTGGTAGCGGCGTCTATTGTGGTGGGTCAATGGTGCATTTCGACGACGACCGGAAAGGGCATGGGTGATGGTCCGATCGGCCTTGCCTTGGCGAAGATGCAGAGGTACTTTTCCGTTGGGCGGCGTTACAGATCTGGAAGGGTGGCCGGAAGTTGGGCTGCAGCTGCTGCTGTTGGAGTTGATGGCAGGGATTTGAAGGAAAAGAGGGTAGCGGCCACCATGATCGGAGGTGCTCGTGTTCGGCTGAACCGGTGGCAGCAGGCGGCGGTGGCAGTTGGGTCGGCAGTGGGGGCATTGCTGGACCCAAGAAGAGCTGACCTGATAGCAGCTCTGGGGGAGACAACTGGGAAGCCGGCATTCGAAAGAATTGTTCAGAGAATGAAGAGTAGCCCTGAAGGCAGAGCCATACTGTTGGAGCAACCCCGTGTAATATCTGCAAATGTGGGTCATGCATGGGATCTTCCGCCCAATACATTTGGTGCTGCCTATGCGAAGTTCATGGGATCTAGGAACTTCTCTCCAGATGATCGACCACCAGTGCGTTTCATGGAAACAGATGAACTTGCATATGTAGCCATGAGGGCTCGTGAGGTGCACGACTTCTGGCACACCCTGTTTGGCCTTCCAACAAACTTGATGGGTGAGTCAGCACTCAAAGTAATAGAGTTTCAGCAAATGTATCTTCCCATGTGCTTGATGTCCGTTATAGGAGGCTCTGTGAGGTTCAATGAGAAGCAAAGAAAATTGTTCTTCCAGCACTACTTTCCTTGGGCCATTCAAGCTGGTATGCAGTCAACAGATCTCATGTGTATTTATTATGAGAAACACTTTCATGAGAATTTGGAGGATGTTCGGAGAAAGTGGGGTATAACTCCTGCTCCTGCTGCACCTAAACAAACCCAAGCAGCAGCAGCTTCACCTGGTCTAGTGGAGAAGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Coq4

Protein Analysis

411

Amino Acids

44.64

Weight (kDa)

9.49

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coq4 PF05019 177 - 392 1.5e-83 Coenzyme Q (ubiquinone) biosynthesis protein Coq4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 249, 275, 302, 415, 506, 557, 560, 758
AclWI GGATC 6 cut(s) 169, 182, 199, 237, 759, 805
AcoI YGGCCR 3 cut(s) 3, 438, 507
AcsI RAATTY 3 cut(s) 18, 82, 1132
AcuI CTGAAG 1 cut(s) 708
AcyI GRCGYC 1 cut(s) 305
AfaI GTAC 1 cut(s) 401
AfiI CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
AgeI ACCGGT 1 cut(s) 543
AgsI TTSAA 4 cut(s) 107, 490, 1021, 1073
AjnI CCWGG 2 cut(s) 158, 1213
AluBI AGCT 5 cut(s) 458, 608, 623, 1076, 1208
AluI AGCT 5 cut(s) 458, 608, 623, 1076, 1208
Alw21I GWGCWC 2 cut(s) 529, 891
Alw26I GTCTC 1 cut(s) 626
Alw44I GTGCAC 1 cut(s) 887
AlwI GGATC 6 cut(s) 169, 182, 199, 237, 759, 805
AlwNI CAGNNNCTG 1 cut(s) 461
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 7 cut(s) 3, 246, 373, 438, 507, 913, 1065
ApaLI GTGCAC 1 cut(s) 887
ApoI RAATTY 3 cut(s) 18, 82, 1132
AsiGI ACCGGT 1 cut(s) 543
AspLEI GCGC 1 cut(s) 280
AspS9I GGNCC 3 cut(s) 365, 595, 1065
AsuHPI GGTGA 7 cut(s) 26, 46, 266, 301, 371, 947, 1203
AsuII TTCGAA 1 cut(s) 654
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 2 cut(s) 365, 595
BaeGI GKGCMC 1 cut(s) 891
BamHI GGATCC 1 cut(s) 174
BanII GRGCYC 1 cut(s) 880
BauI CACGAG 2 cut(s) 528, 879
BbsI GAAGAC 1 cut(s) 153
Bbv12I GWGCWC 2 cut(s) 529, 891
BccI CCATC 4 cut(s) 104, 356, 470, 925
BcgI CGANNNNNNTGC 4 cut(s) 50, 84, 555, 589
BciT130I CCWGG 2 cut(s) 160, 1215
BcoDI GTCTC 1 cut(s) 626
BfaI CTAG 2 cut(s) 801, 1220
BfmI CTRYAG 1 cut(s) 453
BfoI RGCGCY 1 cut(s) 281
BglI GCCNNNNNGGC 2 cut(s) 690, 875
BglII AGATCT 2 cut(s) 425, 1093
Bme1390I CCNGG 2 cut(s) 160, 1215
Bme18I GGWCC 2 cut(s) 365, 595
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 3 cut(s) 365, 595, 1065
BmiI GGNNCC 2 cut(s) 176, 597
BmrFI CCNGG 2 cut(s) 160, 1215
BmrI ACTGGG 1 cut(s) 648
BmsI GCATC 1 cut(s) 381
BmuI ACTGGG 1 cut(s) 648
BpiI GAAGAC 1 cut(s) 153
BplI GAGNNNNNCTC 2 cut(s) 28, 60
BpmI CTGGAG 1 cut(s) 798
Bpu14I TTCGAA 1 cut(s) 654
BsaHI GRCGYC 1 cut(s) 305
BsaJI CCNNGG 4 cut(s) 159, 285, 381, 1061
BsaWI WCCGGW 2 cut(s) 344, 543
BsaXI ACNNNNNCTCC 4 cut(s) 996, 1026, 1156, 1186
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
Bse118I RCCGGY 4 cut(s) 5, 251, 543, 646
Bse1I ACTGG 2 cut(s) 643, 830
Bse3DI GCAATG 1 cut(s) 587
BseBI CCWGG 2 cut(s) 160, 1215
BseDI CCNNGG 4 cut(s) 159, 285, 381, 1061
BseGI GGATG 1 cut(s) 1147
BseLI CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
BseMI GCAATG 1 cut(s) 587
BseNI ACTGG 2 cut(s) 643, 830
BseSI GKGCMC 1 cut(s) 891
BseYI CCCAGC 3 cut(s) 71, 170, 187
BsgI GTGCAG 1 cut(s) 1164
Bsh1285I CGRYCG 2 cut(s) 345, 372
BshFI GGCC 7 cut(s) 5, 248, 375, 440, 509, 915, 1067
BshTI ACCGGT 1 cut(s) 543
BsiEI CGRYCG 2 cut(s) 345, 372
BsiHKAI GWGCWC 2 cut(s) 529, 891
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 6 cut(s) 6, 252, 345, 441, 544, 647
BslI CCNNNNNNNGG 4 cut(s) 94, 382, 414, 447
BsmAI GTCTC 1 cut(s) 626
BsmI GAATGC 1 cut(s) 650
BsnI GGCC 7 cut(s) 5, 248, 375, 440, 509, 915, 1067
BsoBI CYCGRG 1 cut(s) 153
Bsp119I TTCGAA 1 cut(s) 654
Bsp1286I GDGCHC 3 cut(s) 529, 880, 891
BspACI CCGC 8 cut(s) 249, 275, 302, 415, 506, 557, 560, 758
BspANI GGCC 7 cut(s) 5, 248, 375, 440, 509, 915, 1067
BspHI TCATGA 1 cut(s) 1126
BspLI GGNNCC 2 cut(s) 176, 597
BspMAI CTGCAG 1 cut(s) 457
BspPI GGATC 6 cut(s) 169, 182, 199, 237, 759, 805
BspQI GCTCTTC 1 cut(s) 598
BspT104I TTCGAA 1 cut(s) 654
BsrDI GCAATG 1 cut(s) 587
BsrFI RCCGGY 4 cut(s) 5, 251, 543, 646
BsrI ACTGG 2 cut(s) 643, 830
BssAI RCCGGY 4 cut(s) 5, 251, 543, 646
BssECI CCNNGG 4 cut(s) 159, 285, 381, 1061
BssNI GRCGYC 1 cut(s) 305
BssSI CACGAG 2 cut(s) 528, 879
BssT1I CCWWGG 2 cut(s) 381, 1061
Bst2BI CACGAG 2 cut(s) 528, 879
Bst2UI CCWGG 2 cut(s) 160, 1215
Bst4CI ACNGT 5 cut(s) 14, 136, 289, 295, 705
Bst6I CTCTTC 2 cut(s) 598, 671
BstACI GRCGYC 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 461
BstBI TTCGAA 1 cut(s) 654
BstC8I GCNNGC 3 cut(s) 7, 555, 648
BstDEI CTNAG 1 cut(s) 27
BstDSI CCRYGG 1 cut(s) 285
BstF5I GGATG 1 cut(s) 1147
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 1 cut(s) 280
BstMAI GTCTC 1 cut(s) 626
BstMCI CGRYCG 2 cut(s) 345, 372
BstNI CCWGG 2 cut(s) 160, 1215
BstSCI CCNGG 2 cut(s) 158, 1213
BstSFI CTRYAG 1 cut(s) 453
BstSLI GKGCMC 1 cut(s) 891
BstV2I GAAGAC 1 cut(s) 153
BstX2I RGATCY 5 cut(s) 174, 425, 751, 797, 1093
BstXI CCANNNNNNTGG 1 cut(s) 706
BstYI RGATCY 5 cut(s) 174, 425, 751, 797, 1093
BsuRI GGCC 7 cut(s) 5, 248, 375, 440, 509, 915, 1067
BtgI CCRYGG 1 cut(s) 285
BtsCI GGATG 1 cut(s) 1147
BtsI GCAGTG 1 cut(s) 585
BtsIMutI CAGTG 2 cut(s) 585, 837
Cac8I GCNNGC 3 cut(s) 7, 555, 648
CaiI CAGNNNCTG 1 cut(s) 461
CciI TCATGA 1 cut(s) 1126
CfoI GCGC 1 cut(s) 280
Cfr10I RCCGGY 4 cut(s) 5, 251, 543, 646
Cfr13I GGNCC 3 cut(s) 365, 595, 1065
CseI GACGC 1 cut(s) 294
CsiI ACCWGGT 1 cut(s) 1213
Csp6I GTAC 1 cut(s) 400
CspAI ACCGGT 1 cut(s) 543
CviQI GTAC 1 cut(s) 400
DdeI CTNAG 1 cut(s) 27
EaeI YGGCCR 3 cut(s) 3, 438, 507
Eam1104I CTCTTC 2 cut(s) 598, 671
EarI CTCTTC 2 cut(s) 598, 671
EciI GGCGGA 1 cut(s) 747
Eco130I CCWWGG 2 cut(s) 381, 1061
Eco24I GRGCYC 1 cut(s) 880
Eco47I GGWCC 2 cut(s) 365, 595
Eco57I CTGAAG 1 cut(s) 708
Eco88I CYCGRG 1 cut(s) 153
EcoRII CCWGG 2 cut(s) 158, 1213
EcoT14I CCWWGG 2 cut(s) 381, 1061
EcoT22I ATGCAT 1 cut(s) 748
EcoT38I GRGCYC 1 cut(s) 880
ErhI CCWWGG 2 cut(s) 381, 1061
FauNDI CATATG 1 cut(s) 862
FokI GGATG 1 cut(s) 1154
FriOI GRGCYC 1 cut(s) 880
FspBI CTAG 2 cut(s) 801, 1220
GlaI GCGC 1 cut(s) 279
GsaI CCCAGC 3 cut(s) 75, 174, 191
GsuI CTGGAG 1 cut(s) 798
HaeII RGCGCY 1 cut(s) 281
HaeIII GGCC 7 cut(s) 5, 248, 375, 440, 509, 915, 1067
HapII CCGG 6 cut(s) 6, 252, 345, 441, 544, 647
HgaI GACGC 1 cut(s) 294
HhaI GCGC 1 cut(s) 280
Hin1I GRCGYC 1 cut(s) 305
Hin6I GCGC 1 cut(s) 278
HinP1I GCGC 1 cut(s) 278
HincII GTYRAC 2 cut(s) 142, 1089
HindII GTYRAC 2 cut(s) 142, 1089
HinfI GANTC 2 cut(s) 149, 938
HpaII CCGG 6 cut(s) 6, 252, 345, 441, 544, 647
HphI GGTGA 7 cut(s) 26, 46, 266, 301, 371, 947, 1203
Hpy166II GTNNAC 3 cut(s) 142, 889, 1089
Hpy188I TCNGA 7 cut(s) 24, 57, 179, 369, 521, 669, 1149
Hpy188III TCNNGA 7 cut(s) 153, 195, 240, 429, 815, 881, 1127
Hpy8I GTNNAC 3 cut(s) 142, 889, 1089
Hpy99I CGWCG 3 cut(s) 97, 340, 343
HpyAV CCTTC 4 cut(s) 426, 484, 683, 926
HpyCH4III ACNGT 5 cut(s) 14, 136, 289, 295, 705
HpyCH4IV ACGT 1 cut(s) 92
HpyCH4V TGCA 9 cut(s) 330, 394, 455, 731, 746, 860, 889, 1084, 1181
HpyF3I CTNAG 1 cut(s) 27
HpySE526I ACGT 1 cut(s) 92
Hsp92I GRCGYC 1 cut(s) 305
HspAI GCGC 1 cut(s) 278
KroI GCCGGC 2 cut(s) 5, 646
KroNI GCCGGC 2 cut(s) 7, 648
LguI GCTCTTC 1 cut(s) 598
LmnI GCTCC 2 cut(s) 709, 1177
LweI GCATC 1 cut(s) 381
MabI ACCWGGT 1 cut(s) 1213
MaeI CTAG 2 cut(s) 801, 1220
MaeII ACGT 1 cut(s) 92
MaeIII GTNAC 2 cut(s) 289, 419
MboII GAAGA 8 cut(s) 119, 158, 400, 615, 688, 746, 968, 1036
MflI RGATCY 5 cut(s) 174, 425, 751, 797, 1093
MhlI GDGCHC 3 cut(s) 529, 880, 891
MluCI AATT 5 cut(s) 18, 82, 660, 1037, 1132
MlyI GAGTC 2 cut(s) 143, 947
MmeI TCCRAC 3 cut(s) 448, 687, 944
Mph1103I ATGCAT 1 cut(s) 748
MroNI GCCGGC 2 cut(s) 5, 646
MslI CAYNNNNRTG 1 cut(s) 1125
MspA1I CMGCKG 1 cut(s) 458
MspI CCGG 6 cut(s) 6, 252, 345, 441, 544, 647
MspR9I CCNGG 2 cut(s) 160, 1215
Mva1269I GAATGC 1 cut(s) 650
MvaI CCWGG 2 cut(s) 160, 1215
NaeI GCCGGC 2 cut(s) 7, 648
NdeI CATATG 1 cut(s) 862
NgoMIV GCCGGC 2 cut(s) 5, 646
NlaIV GGNNCC 2 cut(s) 176, 597
NmuCI GTSAC 1 cut(s) 289
NsiI ATGCAT 1 cut(s) 748
NspV TTCGAA 1 cut(s) 654
PaeR7I CTCGAG 1 cut(s) 153
PagI TCATGA 1 cut(s) 1126
PciSI GCTCTTC 1 cut(s) 598
PctI GAATGC 1 cut(s) 650
PdiI GCCGGC 2 cut(s) 7, 648
PinAI ACCGGT 1 cut(s) 543
Ple19I CGATCG 1 cut(s) 372
PleI GAGTC 2 cut(s) 143, 946
PpsI GAGTC 2 cut(s) 143, 946
Psp6I CCWGG 2 cut(s) 158, 1213
PspFI CCCAGC 3 cut(s) 71, 170, 187
PspGI CCWGG 2 cut(s) 158, 1213
PspN4I GGNNCC 2 cut(s) 176, 597
PspPI GGNCC 3 cut(s) 365, 595, 1065
PstI CTGCAG 1 cut(s) 457
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 5 cut(s) 174, 425, 751, 797, 1093
PvuI CGATCG 1 cut(s) 372
PvuII CAGCTG 1 cut(s) 458
RsaI GTAC 1 cut(s) 401
RsaNI GTAC 1 cut(s) 400
RseI CAYNNNNRTG 1 cut(s) 1125
SapI GCTCTTC 1 cut(s) 598
Sau96I GGNCC 3 cut(s) 365, 595, 1065
SchI GAGTC 2 cut(s) 143, 947
ScrFI CCNGG 2 cut(s) 160, 1215
SduI GDGCHC 3 cut(s) 529, 880, 891
SexAI ACCWGGT 1 cut(s) 1213
SfaNI GCATC 1 cut(s) 381
SfcI CTRYAG 1 cut(s) 453
Sfr274I CTCGAG 1 cut(s) 153
SfuI TTCGAA 1 cut(s) 654
SgrAI CRCCGGYG 1 cut(s) 251
SinI GGWCC 2 cut(s) 365, 595
SlaI CTCGAG 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 1125
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
Sse9I AATT 5 cut(s) 18, 82, 660, 1037, 1132
SsiI CCGC 8 cut(s) 249, 275, 302, 415, 506, 557, 560, 758
SspMI CTAG 2 cut(s) 801, 1220
StyD4I CCNGG 2 cut(s) 158, 1213
StyI CCWWGG 2 cut(s) 381, 1061
TaaI ACNGT 5 cut(s) 14, 136, 289, 295, 705
TaiI ACGT 1 cut(s) 95
TaqI TCGA 7 cut(s) 60, 123, 154, 232, 335, 654, 823
TasI AATT 5 cut(s) 18, 82, 660, 1037, 1132
TauI GCSGC 6 cut(s) 251, 278, 305, 418, 509, 560
TscAI CASTG 2 cut(s) 585, 837
TseFI GTSAC 1 cut(s) 289
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 5 cut(s) 689, 781, 829, 867, 1115
TspGWI ACGGA 2 cut(s) 397, 985
TspRI CASTG 2 cut(s) 585, 837
VneI GTGCAC 1 cut(s) 887
VpaK11BI GGWCC 2 cut(s) 365, 595
XapI RAATTY 3 cut(s) 18, 82, 1132
XcmI CCANNNNNNNNNTGG 1 cut(s) 292
XhoI CTCGAG 1 cut(s) 153
XspI CTAG 2 cut(s) 801, 1220
Zsp2I ATGCAT 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.