Rh7DG251400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000535
Physical Location & Seq
Forward (+)
26117967 .. 26119024
1058 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG251400.1

Sequence Viewer

Length: 744 bp
ATGGCCGGCAACGGTGAAATTCTGACTAAGGGGGTGAGGCTCTCTCCTTTCTCTCTGATCGACATAGCTGCTGGGCAGCGAAATTTCCCGACGTCGGTTGGGATTGAAGATGGTTTGGAGGTCGATTTTGCTACTGTGGTTGACGAAGACTCTCGAGCCTGGGTTGTTGCTGGGATCCGATTTCTGCTGGGATCAGGATTGGGTCGGCAATCTTTGGTTGTCTTTATGGATCGAGGTTCTAGAAAGGCTGCCGGTGATCTGGTGTTTTTGGTGGCGGCACCTTCCACGGTGACGGTGGTAGCGGCGCCTATTGTGGTGGGTCAATGGTGCATTTCGACGACGACCGGAAAGGGCATGGGTGATGGTCCGATCGGCCTTGCCTTGGCGAAGATGCAGAGGTGCTTTTCCGTTAGGCGCCGTTACAGATCTGGAAGGGTGGCCGGACGTTGGGATGCGGCTGCTGCTATTGGAGTTGATGGCAGGTGCTTTACGGCGGCGGCGCGGGACGGAAGTGGTGGTTATGCAGGTATCACCCACTGGGATGCGTTAGATCGAAGCTCTAACCCTAGGGTTTGGGCTCCTGGCTTTGGGCTTTTTGGGCTCAGGTTTCTTTGGGTGTGGGCTGACCCTATGCATTGGGGTTGCCGGTTTAGGGTGTCTTGGGCCCTAAATACATGTTGCTTTGTTTTTTCAGATGATGGAGTTGAGGGTGATTTTTCATCCTCTTCTCCTAGTACTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

247

Amino Acids

26.35

Weight (kDa)

8.62

Isoelectric Point (pI)

34.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 471
AatII GACGTC 1 cut(s) 95
Acc36I ACCTGC 2 cut(s) 471, 515
AccB1I GGYRCC 3 cut(s) 277, 304, 414
AccII CGCG 1 cut(s) 502
AciI CCGC 6 cut(s) 275, 302, 455, 494, 497, 502
AclWI GGATC 4 cut(s) 169, 182, 199, 237
AcoI YGGCCR 2 cut(s) 3, 438
AcsI RAATTY 2 cut(s) 18, 82
AcyI GRCGYC 3 cut(s) 92, 305, 415
AfaI GTAC 1 cut(s) 736
AfiI CCNNNNNNNGG 5 cut(s) 94, 382, 447, 587, 652
AflIII ACRYGT 1 cut(s) 674
AgsI TTSAA 1 cut(s) 107
AjnI CCWGG 2 cut(s) 158, 580
AluBI AGCT 2 cut(s) 68, 558
AluI AGCT 2 cut(s) 68, 558
AlwI GGATC 4 cut(s) 169, 182, 199, 237
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 4 cut(s) 3, 373, 438, 663
ApaI GGGCCC 1 cut(s) 667
ApeKI GCWGC 5 cut(s) 68, 76, 248, 458, 461
ApoI RAATTY 2 cut(s) 18, 82
AspA2I CCTAGG 1 cut(s) 566
AspLEI GCGC 3 cut(s) 307, 417, 502
AspS9I GGNCC 3 cut(s) 365, 663, 664
AsuHPI GGTGA 7 cut(s) 26, 46, 266, 301, 371, 523, 722
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 1 cut(s) 365
AvrII CCTAGG 1 cut(s) 566
BaeGI GKGCMC 1 cut(s) 667
BamHI GGATCC 1 cut(s) 174
BanI GGYRCC 3 cut(s) 277, 304, 414
BanII GRGCYC 3 cut(s) 580, 603, 667
BbsI GAAGAC 1 cut(s) 153
BbvI GCAGC 5 cut(s) 55, 88, 235, 445, 448
BccI CCATC 4 cut(s) 104, 356, 470, 692
BceAI ACGGC 2 cut(s) 402, 507
BcgI CGANNNNNNTGC 2 cut(s) 50, 84
BciT130I CCWGG 2 cut(s) 160, 582
BfaI CTAG 3 cut(s) 240, 567, 732
BfoI RGCGCY 2 cut(s) 308, 418
BfuAI ACCTGC 2 cut(s) 471, 515
BglII AGATCT 1 cut(s) 425
BlnI CCTAGG 1 cut(s) 566
BmcAI AGTACT 1 cut(s) 736
Bme1390I CCNGG 2 cut(s) 160, 582
Bme18I GGWCC 1 cut(s) 365
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 3 cut(s) 365, 663, 664
BmiI GGNNCC 6 cut(s) 176, 279, 306, 416, 579, 665
BmrFI CCNGG 2 cut(s) 160, 582
BmrI ACTGGG 1 cut(s) 547
BmsI GCATC 3 cut(s) 381, 442, 532
BmuI ACTGGG 1 cut(s) 547
BpiI GAAGAC 1 cut(s) 153
BplI GAGNNNNNCTC 2 cut(s) 28, 60
Bpu10I CCTNAGC 1 cut(s) 602
BsaHI GRCGYC 3 cut(s) 92, 305, 415
BsaJI CCNNGG 4 cut(s) 159, 285, 381, 566
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 5 cut(s) 94, 382, 447, 587, 652
Bse118I RCCGGY 3 cut(s) 5, 251, 645
Bse1I ACTGG 1 cut(s) 542
BseBI CCWGG 2 cut(s) 160, 582
BseDI CCNNGG 4 cut(s) 159, 285, 381, 566
BseGI GGATG 3 cut(s) 457, 547, 719
BseLI CCNNNNNNNGG 5 cut(s) 94, 382, 447, 587, 652
BseMII CTCAG 1 cut(s) 616
BseNI ACTGG 1 cut(s) 542
BseSI GKGCMC 1 cut(s) 667
BseXI GCAGC 5 cut(s) 55, 88, 235, 445, 448
BseYI CCCAGC 3 cut(s) 71, 170, 187
Bsh1236I CGCG 1 cut(s) 502
Bsh1285I CGRYCG 2 cut(s) 345, 372
BshFI GGCC 4 cut(s) 5, 375, 440, 665
BshNI GGYRCC 3 cut(s) 277, 304, 414
BsiEI CGRYCG 2 cut(s) 345, 372
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 5 cut(s) 6, 252, 345, 441, 646
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 5 cut(s) 94, 382, 447, 587, 652
BsmFI GGGAC 1 cut(s) 518
BsnI GGCC 4 cut(s) 5, 375, 440, 665
BsoBI CYCGRG 1 cut(s) 153
Bsp120I GGGCCC 1 cut(s) 663
Bsp1286I GDGCHC 3 cut(s) 580, 603, 667
Bsp143I GATC 8 cut(s) 57, 174, 191, 229, 256, 369, 425, 550
BspACI CCGC 6 cut(s) 275, 302, 455, 494, 497, 502
BspANI GGCC 4 cut(s) 5, 375, 440, 665
BspCNI CTCAG 1 cut(s) 615
BspFNI CGCG 1 cut(s) 502
BspLI GGNNCC 6 cut(s) 176, 279, 306, 416, 579, 665
BspMI ACCTGC 2 cut(s) 471, 515
BspPI GGATC 4 cut(s) 169, 182, 199, 237
BspT107I GGYRCC 3 cut(s) 277, 304, 414
BsrFI RCCGGY 3 cut(s) 5, 251, 645
BsrI ACTGG 1 cut(s) 542
BssAI RCCGGY 3 cut(s) 5, 251, 645
BssECI CCNNGG 4 cut(s) 159, 285, 381, 566
BssMI GATC 8 cut(s) 57, 174, 191, 229, 256, 369, 425, 550
BssNI GRCGYC 3 cut(s) 92, 305, 415
BssT1I CCWWGG 2 cut(s) 381, 566
Bst2UI CCWGG 2 cut(s) 160, 582
Bst4CI ACNGT 4 cut(s) 14, 136, 289, 295
Bst6I CTCTTC 1 cut(s) 730
BstACI GRCGYC 3 cut(s) 92, 305, 415
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 2 cut(s) 27, 602
BstDSI CCRYGG 1 cut(s) 285
BstF5I GGATG 3 cut(s) 457, 547, 719
BstFNI CGCG 1 cut(s) 502
BstH2I RGCGCY 2 cut(s) 308, 418
BstHHI GCGC 3 cut(s) 307, 417, 502
BstKTI GATC 8 cut(s) 60, 177, 194, 232, 259, 372, 428, 553
BstMBI GATC 8 cut(s) 57, 174, 191, 229, 256, 369, 425, 550
BstMCI CGRYCG 2 cut(s) 345, 372
BstMWI GCNNNNNNNGC 2 cut(s) 461, 598
BstNI CCWGG 2 cut(s) 160, 582
BstNSI RCATGY 1 cut(s) 678
BstSCI CCNGG 2 cut(s) 158, 580
BstSLI GKGCMC 1 cut(s) 667
BstUI CGCG 1 cut(s) 502
BstV1I GCAGC 5 cut(s) 55, 88, 235, 445, 448
BstV2I GAAGAC 1 cut(s) 153
BstX2I RGATCY 2 cut(s) 174, 425
BstYI RGATCY 2 cut(s) 174, 425
BsuRI GGCC 4 cut(s) 5, 375, 440, 665
BtgI CCRYGG 1 cut(s) 285
BtsCI GGATG 3 cut(s) 457, 547, 719
BtsIMutI CAGTG 1 cut(s) 535
BveI ACCTGC 2 cut(s) 471, 515
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 3 cut(s) 307, 417, 502
Cfr10I RCCGGY 3 cut(s) 5, 251, 645
Cfr13I GGNCC 3 cut(s) 365, 663, 664
Csp6I GTAC 1 cut(s) 735
CviAII CATG 2 cut(s) 355, 675
CviQI GTAC 1 cut(s) 735
DdeI CTNAG 2 cut(s) 27, 602
DinI GGCGCC 2 cut(s) 306, 416
DpnI GATC 8 cut(s) 59, 176, 193, 231, 258, 371, 427, 552
DpnII GATC 8 cut(s) 57, 174, 191, 229, 256, 369, 425, 550
EaeI YGGCCR 2 cut(s) 3, 438
Eam1104I CTCTTC 1 cut(s) 730
EarI CTCTTC 1 cut(s) 730
Eco130I CCWWGG 2 cut(s) 381, 566
Eco24I GRGCYC 3 cut(s) 580, 603, 667
Eco47I GGWCC 1 cut(s) 365
Eco88I CYCGRG 1 cut(s) 153
EcoO109I RGGNCCY 1 cut(s) 664
EcoRII CCWGG 2 cut(s) 158, 580
EcoT14I CCWWGG 2 cut(s) 381, 566
EcoT22I ATGCAT 1 cut(s) 636
EcoT38I GRGCYC 3 cut(s) 580, 603, 667
EgeI GGCGCC 2 cut(s) 306, 416
EheI GGCGCC 2 cut(s) 306, 416
ErhI CCWWGG 2 cut(s) 381, 566
FaeI CATG 2 cut(s) 358, 678
FaiI YATR 6 cut(s) 65, 227, 356, 522, 632, 676
FaqI GGGAC 1 cut(s) 518
FatI CATG 2 cut(s) 354, 674
FauI CCCGC 1 cut(s) 495
FokI GGATG 3 cut(s) 464, 554, 706
FriOI GRGCYC 3 cut(s) 580, 603, 667
FspBI CTAG 3 cut(s) 240, 567, 732
GlaI GCGC 3 cut(s) 306, 416, 501
GsaI CCCAGC 3 cut(s) 75, 174, 191
HaeII RGCGCY 2 cut(s) 308, 418
HaeIII GGCC 4 cut(s) 5, 375, 440, 665
HapII CCGG 5 cut(s) 6, 252, 345, 441, 646
HhaI GCGC 3 cut(s) 307, 417, 502
Hin1I GRCGYC 3 cut(s) 92, 305, 415
Hin1II CATG 2 cut(s) 358, 678
Hin6I GCGC 3 cut(s) 305, 415, 500
HinP1I GCGC 3 cut(s) 305, 415, 500
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 1 cut(s) 149
HpaII CCGG 5 cut(s) 6, 252, 345, 441, 646
HphI GGTGA 7 cut(s) 26, 46, 266, 301, 371, 523, 722
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 5 cut(s) 24, 57, 179, 369, 694
Hpy188III TCNNGA 5 cut(s) 88, 153, 195, 240, 429
Hpy8I GTNNAC 1 cut(s) 142
Hpy99I CGWCG 4 cut(s) 94, 97, 340, 343
HpyAV CCTTC 2 cut(s) 291, 426
HpyCH4III ACNGT 4 cut(s) 14, 136, 289, 295
HpyCH4IV ACGT 2 cut(s) 92, 445
HpyCH4V TGCA 4 cut(s) 330, 394, 524, 634
HpyF10VI GCNNNNNNNGC 2 cut(s) 461, 598
HpyF3I CTNAG 2 cut(s) 27, 602
HpySE526I ACGT 2 cut(s) 92, 445
Hsp92I GRCGYC 3 cut(s) 92, 305, 415
Hsp92II CATG 2 cut(s) 358, 678
HspAI GCGC 3 cut(s) 305, 415, 500
KasI GGCGCC 2 cut(s) 304, 414
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
Kzo9I GATC 8 cut(s) 57, 174, 191, 229, 256, 369, 425, 550
LmnI GCTCC 1 cut(s) 583
Lsp1109I GCAGC 5 cut(s) 55, 88, 235, 445, 448
LweI GCATC 3 cut(s) 381, 442, 532
MaeI CTAG 3 cut(s) 240, 567, 732
MaeII ACGT 2 cut(s) 92, 445
MaeIII GTNAC 2 cut(s) 289, 419
MalI GATC 8 cut(s) 59, 176, 193, 231, 258, 371, 427, 552
MboI GATC 8 cut(s) 57, 174, 191, 229, 256, 369, 425, 550
MboII GAAGA 4 cut(s) 119, 158, 400, 717
MflI RGATCY 2 cut(s) 174, 425
MhlI GDGCHC 3 cut(s) 580, 603, 667
MluCI AATT 2 cut(s) 18, 82
Mly113I GGCGCC 2 cut(s) 305, 415
MlyI GAGTC 1 cut(s) 143
MnlI CCTC 6 cut(s) 30, 112, 227, 390, 700, 733
Mph1103I ATGCAT 1 cut(s) 636
MroNI GCCGGC 1 cut(s) 5
MseI TTAA 1 cut(s) 742
MslI CAYNNNNRTG 1 cut(s) 540
MspI CCGG 5 cut(s) 6, 252, 345, 441, 646
MspR9I CCNGG 2 cut(s) 160, 582
MvaI CCWGG 2 cut(s) 160, 582
MvnI CGCG 1 cut(s) 502
MwoI GCNNNNNNNGC 2 cut(s) 461, 598
NaeI GCCGGC 1 cut(s) 7
NarI GGCGCC 2 cut(s) 305, 415
NdeII GATC 8 cut(s) 57, 174, 191, 229, 256, 369, 425, 550
NgoMIV GCCGGC 1 cut(s) 5
NlaIII CATG 2 cut(s) 358, 678
NlaIV GGNNCC 6 cut(s) 176, 279, 306, 416, 579, 665
NmuCI GTSAC 1 cut(s) 289
NsiI ATGCAT 1 cut(s) 636
NspI RCATGY 1 cut(s) 678
PaeR7I CTCGAG 1 cut(s) 153
PaqCI CACCTGC 1 cut(s) 471
PciI ACATGT 1 cut(s) 674
PdiI GCCGGC 1 cut(s) 7
Ple19I CGATCG 1 cut(s) 372
PleI GAGTC 1 cut(s) 143
PluTI GGCGCC 2 cut(s) 308, 418
PpsI GAGTC 1 cut(s) 143
PscI ACATGT 1 cut(s) 674
Psp6I CCWGG 2 cut(s) 158, 580
PspFI CCCAGC 3 cut(s) 71, 170, 187
PspGI CCWGG 2 cut(s) 158, 580
PspN4I GGNNCC 6 cut(s) 176, 279, 306, 416, 579, 665
PspOMI GGGCCC 1 cut(s) 663
PspPI GGNCC 3 cut(s) 365, 663, 664
PsuI RGATCY 2 cut(s) 174, 425
PvuI CGATCG 1 cut(s) 372
RsaI GTAC 1 cut(s) 736
RsaNI GTAC 1 cut(s) 735
RseI CAYNNNNRTG 1 cut(s) 540
SaqAI TTAA 1 cut(s) 742
Sau3AI GATC 8 cut(s) 57, 174, 191, 229, 256, 369, 425, 550
Sau96I GGNCC 3 cut(s) 365, 663, 664
ScaI AGTACT 1 cut(s) 736
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 2 cut(s) 160, 582
SduI GDGCHC 3 cut(s) 580, 603, 667
SfaNI GCATC 3 cut(s) 381, 442, 532
SfoI GGCGCC 2 cut(s) 306, 416
Sfr274I CTCGAG 1 cut(s) 153
SinI GGWCC 1 cut(s) 365
SlaI CTCGAG 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 540
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
Sse9I AATT 2 cut(s) 18, 82
SsiI CCGC 6 cut(s) 275, 302, 455, 494, 497, 502
SspDI GGCGCC 2 cut(s) 304, 414
SspMI CTAG 3 cut(s) 240, 567, 732
StyD4I CCNGG 2 cut(s) 158, 580
StyI CCWWGG 2 cut(s) 381, 566
TaaI ACNGT 4 cut(s) 14, 136, 289, 295
TaiI ACGT 2 cut(s) 95, 448
TaqI TCGA 6 cut(s) 60, 123, 154, 232, 335, 553
TasI AATT 2 cut(s) 18, 82
TatI WGTACW 1 cut(s) 734
TauI GCSGC 5 cut(s) 278, 305, 458, 497, 500
Tru1I TTAA 1 cut(s) 742
Tru9I TTAA 1 cut(s) 742
TscAI CASTG 1 cut(s) 542
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 5 cut(s) 68, 76, 248, 458, 461
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 1 cut(s) 708
TspGWI ACGGA 2 cut(s) 397, 522
TspRI CASTG 1 cut(s) 542
VpaK11BI GGWCC 1 cut(s) 365
XapI RAATTY 2 cut(s) 18, 82
XbaI TCTAGA 1 cut(s) 239
XceI RCATGY 1 cut(s) 678
XcmI CCANNNNNNNNNTGG 1 cut(s) 292
XhoI CTCGAG 1 cut(s) 153
XmaJI CCTAGG 1 cut(s) 566
XspI CTAG 3 cut(s) 240, 567, 732
ZraI GACGTC 1 cut(s) 93
ZrmI AGTACT 1 cut(s) 736
Zsp2I ATGCAT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.