Rh1AG327000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000535
Physical Location & Seq
Forward (+)
55993495 .. 55994055
561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG327000.1

Sequence Viewer

Length: 561 bp
ATGGCTATTGCCACAAACTCCCCCTTCTCCGGCGTGTCATGCTTTGTTTTAAGGTGCGCTACAGGTCGTTTCTCTTTGCTTTTCCTTCTCCTTGGACTCGTTGTTAGTGGTTTTGATAGAGGACTTTCCTTGATTCCTGATGGGTCGGGTGATGGAGGTTATGGGGATAACGCAATTGATCCGTGGAGGTGGAGGGGTTTTCTACCCTCTGGTATTCACGTCAATGGCAGCCTAACAACCTGCTTGAGCCCAGAGCATCATCGAGGGCCAGTCGCGGCTGCTGCTGCTGAAGTTGATGGCAGCAATCTGGTGTTGGTGAAGGACGATGCTCTGATTCTGTTTGGGAGTGGTCTGTGCCAGCTTGGTCGTGGTGGCTTGTTGGTGGAATGGTACCAGTGCTTTGCGGCGGCGACGCGGGATGGAGGTGGTGGTTATGCAGATGTCACCCGTTGGGCCGTGTTCGATCGGAGCTCTAACCCTAGCGTTTGGTCTCCTGGCTTTGGGCTTTTTTGGCTCAGATTTTTTTGGGTGTGGGCTGGCCCTATGCTTTGGGGCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

19.88

Weight (kDa)

5.83

Isoelectric Point (pI)

33.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 248
Acc65I GGTACC 1 cut(s) 390
AccB1I GGYRCC 1 cut(s) 390
AccII CGCG 2 cut(s) 275, 415
AciI CCGC 4 cut(s) 275, 404, 407, 415
AclWI GGATC 1 cut(s) 173
AcuI CTGAAG 1 cut(s) 309
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 2 cut(s) 29, 500
AjiI CACGTC 1 cut(s) 220
AjnI CCWGG 1 cut(s) 493
AluBI AGCT 2 cut(s) 361, 471
AluI AGCT 2 cut(s) 361, 471
Alw21I GWGCWC 1 cut(s) 473
Alw26I GTCTC 1 cut(s) 495
AlwI GGATC 1 cut(s) 173
AoxI GGCC 3 cut(s) 266, 453, 538
ApeKI GCWGC 6 cut(s) 228, 278, 281, 284, 300, 555
Asp718I GGTACC 1 cut(s) 390
AspLEI GCGC 1 cut(s) 59
AspS9I GGNCC 3 cut(s) 266, 453, 539
AsuHPI GGTGA 3 cut(s) 161, 328, 436
BanI GGYRCC 1 cut(s) 390
BanII GRGCYC 2 cut(s) 251, 473
Bbv12I GWGCWC 1 cut(s) 473
BbvI GCAGC 6 cut(s) 240, 265, 268, 271, 312, 542
BccI CCATC 4 cut(s) 134, 146, 290, 413
BceAI ACGGC 1 cut(s) 440
BciT130I CCWGG 1 cut(s) 495
BcoDI GTCTC 1 cut(s) 495
BfaI CTAG 2 cut(s) 480, 559
BfmI CTRYAG 1 cut(s) 60
BfuAI ACCTGC 1 cut(s) 248
BisI GCNGC 9 cut(s) 229, 276, 279, 282, 285, 301, 405, 408, 556
BlsI GCNGC 9 cut(s) 230, 277, 280, 283, 286, 302, 406, 409, 557
Bme1390I CCNGG 1 cut(s) 495
BmgBI CACGTC 1 cut(s) 220
BmgT120I GGNCC 3 cut(s) 266, 453, 539
BmiI GGNNCC 1 cut(s) 392
BmrFI CCNGG 1 cut(s) 495
BmsI GCATC 2 cut(s) 265, 316
BpuEI CTTGAG 1 cut(s) 265
BsaI GGTCTC 1 cut(s) 495
BsaJI CCNNGG 2 cut(s) 91, 182
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 500
Bse1I ACTGG 2 cut(s) 269, 394
BseBI CCWGG 1 cut(s) 495
BseDI CCNNGG 2 cut(s) 91, 182
BseGI GGATG 1 cut(s) 424
BseLI CCNNNNNNNGG 2 cut(s) 29, 500
BseMII CTCAG 1 cut(s) 529
BseNI ACTGG 2 cut(s) 269, 394
BseXI GCAGC 6 cut(s) 240, 265, 268, 271, 312, 542
Bsh1236I CGCG 2 cut(s) 275, 415
Bsh1285I CGRYCG 1 cut(s) 466
BshFI GGCC 3 cut(s) 268, 455, 540
BshNI GGYRCC 1 cut(s) 390
BsiEI CGRYCG 1 cut(s) 466
BsiHKAI GWGCWC 1 cut(s) 473
BsiSI CCGG 1 cut(s) 30
BslI CCNNNNNNNGG 2 cut(s) 29, 500
BsmAI GTCTC 1 cut(s) 495
BsnI GGCC 3 cut(s) 268, 455, 540
Bso31I GGTCTC 1 cut(s) 495
Bsp1286I GDGCHC 2 cut(s) 251, 473
Bsp143I GATC 2 cut(s) 178, 463
BspACI CCGC 4 cut(s) 275, 404, 407, 415
BspANI GGCC 3 cut(s) 268, 455, 540
BspCNI CTCAG 1 cut(s) 528
BspFNI CGCG 2 cut(s) 275, 415
BspLI GGNNCC 1 cut(s) 392
BspMI ACCTGC 1 cut(s) 248
BspPI GGATC 1 cut(s) 173
BspT107I GGYRCC 1 cut(s) 390
BspTNI GGTCTC 1 cut(s) 495
BsrI ACTGG 2 cut(s) 269, 394
BssECI CCNNGG 2 cut(s) 91, 182
BssMI GATC 2 cut(s) 178, 463
BssT1I CCWWGG 1 cut(s) 91
Bst2UI CCWGG 1 cut(s) 495
BstC8I GCNNGC 2 cut(s) 359, 538
BstDEI CTNAG 1 cut(s) 515
BstDSI CCRYGG 1 cut(s) 182
BstF5I GGATG 1 cut(s) 424
BstFNI CGCG 2 cut(s) 275, 415
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 2 cut(s) 181, 466
BstMAI GTCTC 1 cut(s) 495
BstMBI GATC 2 cut(s) 178, 463
BstMCI CGRYCG 1 cut(s) 466
BstMWI GCNNNNNNNGC 4 cut(s) 39, 281, 284, 511
BstNI CCWGG 1 cut(s) 495
BstSCI CCNGG 1 cut(s) 493
BstSFI CTRYAG 1 cut(s) 60
BstUI CGCG 2 cut(s) 275, 415
BstV1I GCAGC 6 cut(s) 240, 265, 268, 271, 312, 542
BsuRI GGCC 3 cut(s) 268, 455, 540
BtgI CCRYGG 1 cut(s) 182
BtrI CACGTC 1 cut(s) 220
BtsCI GGATG 1 cut(s) 424
BtsIMutI CAGTG 1 cut(s) 401
BveI ACCTGC 1 cut(s) 248
Cac8I GCNNGC 2 cut(s) 359, 538
CfoI GCGC 1 cut(s) 59
Cfr13I GGNCC 3 cut(s) 266, 453, 539
CseI GACGC 1 cut(s) 421
Csp6I GTAC 1 cut(s) 391
CviAII CATG 1 cut(s) 39
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 1 cut(s) 515
DpnI GATC 2 cut(s) 180, 465
DpnII GATC 2 cut(s) 178, 463
Ecl136II GAGCTC 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 91
Eco24I GRGCYC 2 cut(s) 251, 473
Eco31I GGTCTC 1 cut(s) 495
Eco53kI GAGCTC 1 cut(s) 471
Eco57I CTGAAG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 471
EcoRII CCWGG 1 cut(s) 493
EcoT14I CCWWGG 1 cut(s) 91
EcoT38I GRGCYC 2 cut(s) 251, 473
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 1 cut(s) 42
FaiI YATR 4 cut(s) 40, 162, 435, 545
FatI CATG 1 cut(s) 38
FauI CCCGC 1 cut(s) 408
Fnu4HI GCNGC 9 cut(s) 229, 276, 279, 282, 285, 301, 405, 408, 556
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 2 cut(s) 251, 473
Fsp4HI GCNGC 9 cut(s) 229, 276, 279, 282, 285, 301, 405, 408, 556
FspBI CTAG 2 cut(s) 480, 559
GlaI GCGC 1 cut(s) 58
GluI GCNGC 9 cut(s) 229, 276, 279, 282, 285, 301, 405, 408, 556
HaeIII GGCC 3 cut(s) 268, 455, 540
HapII CCGG 1 cut(s) 30
HgaI GACGC 1 cut(s) 421
HhaI GCGC 1 cut(s) 59
Hin1II CATG 1 cut(s) 42
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HinfI GANTC 3 cut(s) 96, 133, 334
HpaII CCGG 1 cut(s) 30
HphI GGTGA 3 cut(s) 161, 328, 436
Hpy188I TCNGA 3 cut(s) 333, 468, 518
Hpy188III TCNNGA 1 cut(s) 137
Hpy99I CGWCG 1 cut(s) 415
HpyAV CCTTC 3 cut(s) 34, 95, 313
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 1 cut(s) 437
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 281, 284, 511
HpyF3I CTNAG 1 cut(s) 515
HpySE526I ACGT 1 cut(s) 219
Hsp92II CATG 1 cut(s) 42
HspAI GCGC 1 cut(s) 57
KpnI GGTACC 1 cut(s) 394
Kzo9I GATC 2 cut(s) 178, 463
LmnI GCTCC 1 cut(s) 468
Lsp1109I GCAGC 6 cut(s) 240, 265, 268, 271, 312, 542
LweI GCATC 2 cut(s) 265, 316
MaeI CTAG 2 cut(s) 480, 559
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 1 cut(s) 442
MalI GATC 2 cut(s) 180, 465
MboI GATC 2 cut(s) 178, 463
MfeI CAATTG 1 cut(s) 174
MhlI GDGCHC 2 cut(s) 251, 473
MluCI AATT 1 cut(s) 174
MlyI GAGTC 1 cut(s) 90
MnlI CCTC 7 cut(s) 113, 149, 180, 186, 217, 257, 416
MseI TTAA 1 cut(s) 50
MslI CAYNNNNRTG 1 cut(s) 222
MspI CCGG 1 cut(s) 30
MspR9I CCNGG 1 cut(s) 495
MunI CAATTG 1 cut(s) 174
MvaI CCWGG 1 cut(s) 495
MvnI CGCG 2 cut(s) 275, 415
MwoI GCNNNNNNNGC 4 cut(s) 39, 281, 284, 511
NdeII GATC 2 cut(s) 178, 463
NlaIII CATG 1 cut(s) 42
NlaIV GGNNCC 1 cut(s) 392
NmuCI GTSAC 1 cut(s) 442
PfeI GAWTC 2 cut(s) 133, 334
PkrI GCNGC 9 cut(s) 230, 277, 280, 283, 286, 302, 406, 409, 557
Ple19I CGATCG 1 cut(s) 466
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 473
Psp6I CCWGG 1 cut(s) 493
PspGI CCWGG 1 cut(s) 493
PspN4I GGNNCC 1 cut(s) 392
PspPI GGNCC 3 cut(s) 266, 453, 539
PvuI CGATCG 1 cut(s) 466
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
RseI CAYNNNNRTG 1 cut(s) 222
SacI GAGCTC 1 cut(s) 473
SaqAI TTAA 1 cut(s) 50
SatI GCNGC 9 cut(s) 229, 276, 279, 282, 285, 301, 405, 408, 556
Sau3AI GATC 2 cut(s) 178, 463
Sau96I GGNCC 3 cut(s) 266, 453, 539
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 1 cut(s) 495
SduI GDGCHC 2 cut(s) 251, 473
SetI ASST 9 cut(s) 56, 67, 160, 191, 222, 242, 363, 427, 473
SfaNI GCATC 2 cut(s) 265, 316
SfcI CTRYAG 1 cut(s) 60
SmiMI CAYNNNNRTG 1 cut(s) 222
SmlI CTYRAG 1 cut(s) 244
SmoI CTYRAG 1 cut(s) 244
Sse9I AATT 1 cut(s) 174
SsiI CCGC 4 cut(s) 275, 404, 407, 415
SspMI CTAG 2 cut(s) 480, 559
SstI GAGCTC 1 cut(s) 473
StyD4I CCNGG 1 cut(s) 493
StyI CCWWGG 1 cut(s) 91
TaiI ACGT 1 cut(s) 222
TaqI TCGA 2 cut(s) 262, 462
TasI AATT 1 cut(s) 174
TauI GCSGC 3 cut(s) 278, 407, 410
TfiI GAWTC 2 cut(s) 133, 334
Tru1I TTAA 1 cut(s) 50
Tru9I TTAA 1 cut(s) 50
TscAI CASTG 1 cut(s) 401
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 6 cut(s) 228, 278, 281, 284, 300, 555
Tsp45I GTSAC 1 cut(s) 442
TspGWI ACGGA 1 cut(s) 171
TspRI CASTG 1 cut(s) 401
XcmI CCANNNNNNNNNTGG 1 cut(s) 365
XspI CTAG 2 cut(s) 480, 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.