Rw2G017250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000535
Physical Location & Seq
Reverse (-)
21264759 .. 21266250
1492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G017250.1

Sequence Viewer

Length: 462 bp
ATGGCTGCTGGGCAGCGAAATTTCCCGACGTCGGTTGGGATTGAAGATGGTTTGGAGGTCGATTTTGCTACTGTGGTTGACGAAGACTCTCGAGCCTGGGTTGTTGTTGGGATCCGATTTCTGCTGGGATCAGGATTGGGTCGGCAATCTTTGTTTGTCTTTATGGATCGAGGTTCTGGAAAGGCCGCCGGTGATCTAGTGTTTTTGGTGGTGGCGCCTTCCACGGTGACGGTGGTAGCGGTGTCTATTGTGGTGGGTCAATGGTGCATTTCGACGACGACCGGAAAGGGCATGGGTGATGGTCCGATCGGCCTTGCCTTGGCGAAGATGCAGAGGTACTTTTCCGTTGGGCGGCGTTACAGATCTGGAAGGGTGGCCGGACGTTGGGCTGCGGCTGCTGCTGTTGGAGTTGATGGCAGCACACTGCTCCCTACTGAAAGTAATACTCTTCATCTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

16.04

Weight (kDa)

8.97

Isoelectric Point (pI)

26.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 32
AccB1I GGYRCC 1 cut(s) 214
AciI CCGC 4 cut(s) 186, 239, 352, 392
AclWI GGATC 4 cut(s) 106, 119, 136, 174
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 1 cut(s) 19
AcyI GRCGYC 2 cut(s) 29, 215
AfaI GTAC 1 cut(s) 338
AfiI CCNNNNNNNGG 4 cut(s) 31, 319, 351, 384
AgsI TTSAA 1 cut(s) 44
AjnI CCWGG 1 cut(s) 95
AlwI GGATC 4 cut(s) 106, 119, 136, 174
Ama87I CYCGRG 1 cut(s) 90
AoxI GGCC 3 cut(s) 183, 310, 375
ApeKI GCWGC 6 cut(s) 5, 13, 389, 395, 398, 417
ApoI RAATTY 1 cut(s) 19
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 3 cut(s) 203, 238, 308
AvaI CYCGRG 1 cut(s) 90
AvaII GGWCC 1 cut(s) 302
BamHI GGATCC 1 cut(s) 111
BanI GGYRCC 1 cut(s) 214
BbsI GAAGAC 1 cut(s) 90
BbvI GCAGC 5 cut(s) 25, 376, 382, 385, 429
BccI CCATC 3 cut(s) 41, 293, 407
BciT130I CCWGG 1 cut(s) 97
BfaI CTAG 1 cut(s) 197
BfoI RGCGCY 1 cut(s) 218
BglII AGATCT 1 cut(s) 362
BisI GCNGC 9 cut(s) 6, 14, 186, 353, 390, 393, 396, 399, 418
BlsI GCNGC 9 cut(s) 7, 15, 187, 354, 391, 394, 397, 400, 419
Bme1390I CCNGG 1 cut(s) 97
Bme18I GGWCC 1 cut(s) 302
BmeT110I CYCGRG 1 cut(s) 90
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 2 cut(s) 113, 216
BmrFI CCNGG 1 cut(s) 97
BmsI GCATC 1 cut(s) 318
BpiI GAAGAC 1 cut(s) 90
BsaHI GRCGYC 2 cut(s) 29, 215
BsaJI CCNNGG 3 cut(s) 96, 222, 318
BsaWI WCCGGW 1 cut(s) 281
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 319, 351, 384
Bse118I RCCGGY 1 cut(s) 188
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 3 cut(s) 96, 222, 318
BseLI CCNNNNNNNGG 4 cut(s) 31, 319, 351, 384
BseXI GCAGC 5 cut(s) 25, 376, 382, 385, 429
BseYI CCCAGC 2 cut(s) 8, 124
Bsh1285I CGRYCG 2 cut(s) 282, 309
BshFI GGCC 3 cut(s) 185, 312, 377
BshNI GGYRCC 1 cut(s) 214
BsiEI CGRYCG 2 cut(s) 282, 309
BsiHKCI CYCGRG 1 cut(s) 90
BsiSI CCGG 3 cut(s) 189, 282, 378
BslI CCNNNNNNNGG 4 cut(s) 31, 319, 351, 384
BsnI GGCC 3 cut(s) 185, 312, 377
BsoBI CYCGRG 1 cut(s) 90
Bsp143I GATC 6 cut(s) 111, 128, 166, 193, 306, 362
BspACI CCGC 4 cut(s) 186, 239, 352, 392
BspANI GGCC 3 cut(s) 185, 312, 377
BspLI GGNNCC 2 cut(s) 113, 216
BspPI GGATC 4 cut(s) 106, 119, 136, 174
BspT107I GGYRCC 1 cut(s) 214
BsrFI RCCGGY 1 cut(s) 188
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 3 cut(s) 96, 222, 318
BssMI GATC 6 cut(s) 111, 128, 166, 193, 306, 362
BssNI GRCGYC 2 cut(s) 29, 215
BssT1I CCWWGG 1 cut(s) 318
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 3 cut(s) 73, 226, 232
Bst6I CTCTTC 1 cut(s) 453
BstACI GRCGYC 2 cut(s) 29, 215
BstDSI CCRYGG 1 cut(s) 222
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 6 cut(s) 114, 131, 169, 196, 309, 365
BstMBI GATC 6 cut(s) 111, 128, 166, 193, 306, 362
BstMCI CGRYCG 2 cut(s) 282, 309
BstMWI GCNNNNNNNGC 2 cut(s) 395, 398
BstNI CCWGG 1 cut(s) 97
BstSCI CCNGG 1 cut(s) 95
BstV1I GCAGC 5 cut(s) 25, 376, 382, 385, 429
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 2 cut(s) 111, 362
BstYI RGATCY 2 cut(s) 111, 362
BsuRI GGCC 3 cut(s) 185, 312, 377
BtgI CCRYGG 1 cut(s) 222
BtsI GCAGTG 1 cut(s) 422
BtsIMutI CAGTG 1 cut(s) 422
CfoI GCGC 1 cut(s) 217
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 1 cut(s) 302
Csp6I GTAC 1 cut(s) 337
CviAII CATG 1 cut(s) 292
CviJI RGCY 7 cut(s) 5, 95, 185, 312, 377, 389, 395
CviKI_1 RGCY 7 cut(s) 5, 95, 185, 312, 377, 389, 395
CviQI GTAC 1 cut(s) 337
DinI GGCGCC 1 cut(s) 216
DpnI GATC 6 cut(s) 113, 130, 168, 195, 308, 364
DpnII GATC 6 cut(s) 111, 128, 166, 193, 306, 362
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
Eco130I CCWWGG 1 cut(s) 318
Eco47I GGWCC 1 cut(s) 302
Eco88I CYCGRG 1 cut(s) 90
EcoRII CCWGG 1 cut(s) 95
EcoT14I CCWWGG 1 cut(s) 318
EgeI GGCGCC 1 cut(s) 216
EheI GGCGCC 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 318
FaeI CATG 1 cut(s) 295
FaiI YATR 2 cut(s) 164, 293
FatI CATG 1 cut(s) 291
Fnu4HI GCNGC 9 cut(s) 6, 14, 186, 353, 390, 393, 396, 399, 418
Fsp4HI GCNGC 9 cut(s) 6, 14, 186, 353, 390, 393, 396, 399, 418
FspBI CTAG 1 cut(s) 197
GlaI GCGC 1 cut(s) 216
GluI GCNGC 9 cut(s) 6, 14, 186, 353, 390, 393, 396, 399, 418
GsaI CCCAGC 2 cut(s) 12, 128
HaeII RGCGCY 1 cut(s) 218
HaeIII GGCC 3 cut(s) 185, 312, 377
HapII CCGG 3 cut(s) 189, 282, 378
HhaI GCGC 1 cut(s) 217
Hin1I GRCGYC 2 cut(s) 29, 215
Hin1II CATG 1 cut(s) 295
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 1 cut(s) 86
HpaII CCGG 3 cut(s) 189, 282, 378
HphI GGTGA 3 cut(s) 203, 238, 308
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 2 cut(s) 116, 306
Hpy188III TCNNGA 5 cut(s) 25, 90, 132, 177, 366
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 4 cut(s) 31, 34, 277, 280
HpyAV CCTTC 2 cut(s) 228, 363
HpyCH4III ACNGT 3 cut(s) 73, 226, 232
HpyCH4IV ACGT 2 cut(s) 29, 382
HpyCH4V TGCA 2 cut(s) 267, 331
HpyF10VI GCNNNNNNNGC 2 cut(s) 395, 398
HpySE526I ACGT 2 cut(s) 29, 382
Hsp92I GRCGYC 2 cut(s) 29, 215
Hsp92II CATG 1 cut(s) 295
HspAI GCGC 1 cut(s) 215
KasI GGCGCC 1 cut(s) 214
Kzo9I GATC 6 cut(s) 111, 128, 166, 193, 306, 362
LmnI GCTCC 1 cut(s) 432
LpnPI CCDG 9 cut(s) 82, 109, 110, 117, 162, 202, 295, 351, 391
Lsp1109I GCAGC 5 cut(s) 25, 376, 382, 385, 429
LweI GCATC 1 cut(s) 318
MaeI CTAG 1 cut(s) 197
MaeII ACGT 2 cut(s) 29, 382
MaeIII GTNAC 2 cut(s) 226, 356
MalI GATC 6 cut(s) 113, 130, 168, 195, 308, 364
MboI GATC 6 cut(s) 111, 128, 166, 193, 306, 362
MboII GAAGA 4 cut(s) 56, 95, 337, 440
MflI RGATCY 2 cut(s) 111, 362
MluCI AATT 1 cut(s) 19
Mly113I GGCGCC 1 cut(s) 215
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 1 cut(s) 385
MnlI CCTC 3 cut(s) 49, 164, 327
MspI CCGG 3 cut(s) 189, 282, 378
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
MwoI GCNNNNNNNGC 2 cut(s) 395, 398
NarI GGCGCC 1 cut(s) 215
NdeII GATC 6 cut(s) 111, 128, 166, 193, 306, 362
NlaIII CATG 1 cut(s) 295
NlaIV GGNNCC 2 cut(s) 113, 216
NmuCI GTSAC 1 cut(s) 226
PaeR7I CTCGAG 1 cut(s) 90
PkrI GCNGC 9 cut(s) 7, 15, 187, 354, 391, 394, 397, 400, 419
Ple19I CGATCG 1 cut(s) 309
PleI GAGTC 1 cut(s) 80
PluTI GGCGCC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 80
Psp6I CCWGG 1 cut(s) 95
PspFI CCCAGC 2 cut(s) 8, 124
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 2 cut(s) 113, 216
PspPI GGNCC 1 cut(s) 302
PsuI RGATCY 2 cut(s) 111, 362
PvuI CGATCG 1 cut(s) 309
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
SatI GCNGC 9 cut(s) 6, 14, 186, 353, 390, 393, 396, 399, 418
Sau3AI GATC 6 cut(s) 111, 128, 166, 193, 306, 362
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 1 cut(s) 97
SetI ASST 5 cut(s) 32, 60, 175, 338, 385
SfaNI GCATC 1 cut(s) 318
SfoI GGCGCC 1 cut(s) 216
Sfr274I CTCGAG 1 cut(s) 90
SgrAI CRCCGGYG 1 cut(s) 188
SinI GGWCC 1 cut(s) 302
SlaI CTCGAG 1 cut(s) 90
SmlI CTYRAG 1 cut(s) 90
SmoI CTYRAG 1 cut(s) 90
Sse9I AATT 1 cut(s) 19
SsiI CCGC 4 cut(s) 186, 239, 352, 392
SspDI GGCGCC 1 cut(s) 214
SspMI CTAG 1 cut(s) 197
StyD4I CCNGG 1 cut(s) 95
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 3 cut(s) 73, 226, 232
TaiI ACGT 2 cut(s) 32, 385
TaqI TCGA 4 cut(s) 60, 91, 169, 272
TasI AATT 1 cut(s) 19
TauI GCSGC 3 cut(s) 188, 355, 395
TscAI CASTG 1 cut(s) 429
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 6 cut(s) 5, 13, 389, 395, 398, 417
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 1 cut(s) 440
TspGWI ACGGA 1 cut(s) 334
TspRI CASTG 1 cut(s) 429
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 1 cut(s) 19
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
XhoI CTCGAG 1 cut(s) 90
XspI CTAG 1 cut(s) 197
ZraI GACGTC 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.