Rh7DG257100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000535
Physical Location & Seq
Reverse (-)
26916584 .. 26916832
249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG257100.1

Sequence Viewer

Length: 249 bp
ATGGGCTTCCACCGGATCTTATCTGGTGTAGCTATGCGCTGGAGATTGTGGAAGCCAGGAGCAATTAACGACGCCCTCTGGCTTACAGAGGCAAAGTATGATGCCCGAGTACTGGATCGTATGATGCCCGAGTACTGGATCGAGCTTCCGGTGTTGGAGGCGGCACTGGGAGTGCGGCTGAAAGCCTGTTGCTGGTGGCGGCAGCAGTCTCAGTTGGATTTGGGCCTTTACCAACTTGGGGCCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

82

Amino Acids

9.64

Weight (kDa)

8.71

Isoelectric Point (pI)

62.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 161, 175, 199
AclWI GGATC 3 cut(s) 23, 123, 146
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 2 cut(s) 111, 134
AfiI CCNNNNNNNGG 4 cut(s) 112, 135, 192, 238
AjnI CCWGG 1 cut(s) 55
AluBI AGCT 2 cut(s) 32, 145
AluI AGCT 2 cut(s) 32, 145
Alw26I GTCTC 1 cut(s) 213
AlwI GGATC 3 cut(s) 23, 123, 146
Ama87I CYCGRG 2 cut(s) 105, 128
AoxI GGCC 2 cut(s) 223, 240
ApeKI GCWGC 1 cut(s) 202
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 2 cut(s) 223, 240
AvaI CYCGRG 2 cut(s) 105, 128
BbvI GCAGC 1 cut(s) 214
BciT130I CCWGG 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 213
BfaI CTAG 1 cut(s) 247
BisI GCNGC 4 cut(s) 162, 176, 200, 203
BlsI GCNGC 4 cut(s) 163, 177, 201, 204
BmcAI AGTACT 2 cut(s) 111, 134
Bme1390I CCNGG 1 cut(s) 57
BmeT110I CYCGRG 2 cut(s) 105, 128
BmgT120I GGNCC 2 cut(s) 223, 240
BmiI GGNNCC 1 cut(s) 241
BmrFI CCNGG 1 cut(s) 57
BmrI ACTGGG 1 cut(s) 176
BmsI GCATC 2 cut(s) 91, 114
BmuI ACTGGG 1 cut(s) 176
BpmI CTGGAG 1 cut(s) 61
BsaHI GRCGYC 1 cut(s) 72
BsaWI WCCGGW 2 cut(s) 12, 148
Bsc4I CCNNNNNNNGG 4 cut(s) 112, 135, 192, 238
Bse1I ACTGG 3 cut(s) 117, 140, 171
BseBI CCWGG 1 cut(s) 57
BseLI CCNNNNNNNGG 4 cut(s) 112, 135, 192, 238
BseMII CTCAG 1 cut(s) 224
BseNI ACTGG 3 cut(s) 117, 140, 171
BseXI GCAGC 1 cut(s) 214
BshFI GGCC 2 cut(s) 225, 242
BsiHKCI CYCGRG 2 cut(s) 105, 128
BsiSI CCGG 2 cut(s) 13, 149
BslI CCNNNNNNNGG 4 cut(s) 112, 135, 192, 238
BsmAI GTCTC 1 cut(s) 213
BsnI GGCC 2 cut(s) 225, 242
BsoBI CYCGRG 2 cut(s) 105, 128
Bsp143I GATC 3 cut(s) 15, 115, 138
BspACI CCGC 3 cut(s) 161, 175, 199
BspANI GGCC 2 cut(s) 225, 242
BspCNI CTCAG 1 cut(s) 223
BspLI GGNNCC 1 cut(s) 241
BspPI GGATC 3 cut(s) 23, 123, 146
BsrI ACTGG 3 cut(s) 117, 140, 171
BssMI GATC 3 cut(s) 15, 115, 138
BssNI GRCGYC 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 57
BstACI GRCGYC 1 cut(s) 72
BstDEI CTNAG 1 cut(s) 210
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 3 cut(s) 18, 118, 141
BstMAI GTCTC 1 cut(s) 213
BstMBI GATC 3 cut(s) 15, 115, 138
BstNI CCWGG 1 cut(s) 57
BstSCI CCNGG 1 cut(s) 55
BstV1I GCAGC 1 cut(s) 214
BstX2I RGATCY 1 cut(s) 15
BstYI RGATCY 1 cut(s) 15
BsuRI GGCC 2 cut(s) 225, 242
BtsIMutI CAGTG 1 cut(s) 164
CfoI GCGC 1 cut(s) 39
Cfr13I GGNCC 2 cut(s) 223, 240
CseI GACGC 1 cut(s) 80
Csp6I GTAC 2 cut(s) 110, 133
CviJI RGCY 9 cut(s) 6, 32, 55, 82, 145, 178, 185, 225, 242
CviKI_1 RGCY 9 cut(s) 6, 32, 55, 82, 145, 178, 185, 225, 242
CviQI GTAC 2 cut(s) 110, 133
DdeI CTNAG 1 cut(s) 210
DpnI GATC 3 cut(s) 17, 117, 140
DpnII GATC 3 cut(s) 15, 115, 138
Eco88I CYCGRG 2 cut(s) 105, 128
EcoO109I RGGNCCY 1 cut(s) 240
EcoRII CCWGG 1 cut(s) 55
FaiI YATR 3 cut(s) 35, 99, 122
Fnu4HI GCNGC 4 cut(s) 162, 176, 200, 203
Fsp4HI GCNGC 4 cut(s) 162, 176, 200, 203
FspBI CTAG 1 cut(s) 247
GlaI GCGC 1 cut(s) 38
GluI GCNGC 4 cut(s) 162, 176, 200, 203
GsuI CTGGAG 1 cut(s) 61
HaeIII GGCC 2 cut(s) 225, 242
HapII CCGG 2 cut(s) 13, 149
HgaI GACGC 1 cut(s) 80
HhaI GCGC 1 cut(s) 39
Hin1I GRCGYC 1 cut(s) 72
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HpaII CCGG 2 cut(s) 13, 149
Hpy99I CGWCG 1 cut(s) 74
HpyF3I CTNAG 1 cut(s) 210
Hsp92I GRCGYC 1 cut(s) 72
HspAI GCGC 1 cut(s) 37
Kzo9I GATC 3 cut(s) 15, 115, 138
LmnI GCTCC 1 cut(s) 59
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 2 cut(s) 91, 114
MaeI CTAG 1 cut(s) 247
MalI GATC 3 cut(s) 17, 117, 140
MboI GATC 3 cut(s) 15, 115, 138
MflI RGATCY 1 cut(s) 15
MluCI AATT 1 cut(s) 63
MmeI TCCRAC 2 cut(s) 135, 195
MnlI CCTC 3 cut(s) 82, 86, 151
MseI TTAA 1 cut(s) 66
MspI CCGG 2 cut(s) 13, 149
MspR9I CCNGG 1 cut(s) 57
MvaI CCWGG 1 cut(s) 57
NdeII GATC 3 cut(s) 15, 115, 138
NlaIV GGNNCC 1 cut(s) 241
PkrI GCNGC 4 cut(s) 163, 177, 201, 204
Psp6I CCWGG 1 cut(s) 55
PspGI CCWGG 1 cut(s) 55
PspN4I GGNNCC 1 cut(s) 241
PspPI GGNCC 2 cut(s) 223, 240
PsuI RGATCY 1 cut(s) 15
RsaI GTAC 2 cut(s) 111, 134
RsaNI GTAC 2 cut(s) 110, 133
SaqAI TTAA 1 cut(s) 66
SatI GCNGC 4 cut(s) 162, 176, 200, 203
Sau3AI GATC 3 cut(s) 15, 115, 138
Sau96I GGNCC 2 cut(s) 223, 240
ScaI AGTACT 2 cut(s) 111, 134
ScrFI CCNGG 1 cut(s) 57
SetI ASST 2 cut(s) 34, 147
SfaNI GCATC 2 cut(s) 91, 114
Sse9I AATT 1 cut(s) 63
SsiI CCGC 3 cut(s) 161, 175, 199
SspMI CTAG 1 cut(s) 247
StyD4I CCNGG 1 cut(s) 55
TaqI TCGA 1 cut(s) 141
TasI AATT 1 cut(s) 63
TatI WGTACW 2 cut(s) 109, 132
TauI GCSGC 3 cut(s) 164, 178, 202
Tru1I TTAA 1 cut(s) 66
Tru9I TTAA 1 cut(s) 66
TscAI CASTG 1 cut(s) 171
TseI GCWGC 1 cut(s) 202
TspRI CASTG 1 cut(s) 171
XspI CTAG 1 cut(s) 247
ZrmI AGTACT 2 cut(s) 111, 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.